2023/02/05 更新

写真a

スギヤマ サヤカ
杉山 清佳
SUGIYAMA Sayaka
所属
教育研究院 医歯学系 医学系列 教授
医歯学総合研究科 生体機能調節医学専攻 機能再建医学 教授
職名
教授
外部リンク

学位

  • 博士(医学) ( 2001年3月   東北大学 )

研究キーワード

  • 臨界期

  • Parvalbumin

  • 包括脳ネットワーク

  • 可塑性

  • 眼優位性

  • homeoprotein

  • GABA

  • 視覚野

  • Otx2

研究分野

  • ライフサイエンス / 神経科学一般

  • ライフサイエンス / 神経科学一般

経歴(researchmap)

  • 新潟大学   大学院医歯学総合研究科   教授

    2022年4月 - 現在

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  • 新潟大学   医歯学系 神経発達学分野   准教授(PI)

    2014年4月 - 2022年3月

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  • 新潟大学 医歯学系 神経発達研究室   テニュアトラック准教授(PI)

    2009年10月 - 2014年3月

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  • ハーバード大学小児病院   JSPS特別研究員・海外特別研究員

    2006年 - 2009年

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  • 独立行政法人理化学研究所 神経回路発達研究チーム   研究員

    2005年

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  • 独立行政法人理化学研究所 神経回路発達研究チーム   基礎科学特別研究員

    2002年 - 2005年

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  • 東北大学加齢研 分子神経研究分野   JSPS特別研究員

    1999年 - 2002年

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▶ 全件表示

経歴

  • 新潟大学   医歯学総合研究科 生体機能調節医学専攻 機能再建医学   教授

    2022年4月 - 現在

  • 新潟大学   教育研究院 医歯学系 医学系列   教授

    2022年4月 - 現在

  • 新潟大学   医歯学総合研究科 生体機能調節医学専攻 感覚統合医学   准教授

    2014年4月 - 2022年3月

  • 新潟大学   経営戦略本部 若手研究者育成推進室   准教授

    2009年10月 - 2014年3月

所属学協会

委員歴

  • 日本神経科学学会   電子ジャーナル検討委員  

    2015年 - 2016年   

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    団体区分:学協会

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  • 日本神経科学学会   ダイバーシティ対応委員  

    2013年6月 - 現在   

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    団体区分:学協会

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論文

  • Coactosin Promotes F-Actin Protrusion in Growth Cones Under Cofilin-Related Signaling Pathway

    Xubin Hou, Motohiro Nozumi, Harukazu Nakamura, Michihiro Igarashi, Sayaka Sugiyama

    Frontiers in Cell and Developmental Biology   9   2021年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Media SA  

    During brain development, axon outgrowth and its subsequent pathfinding are reliant on a highly motile growth cone located at the tip of the axon. Actin polymerization that is regulated by actin-depolymerizing factors homology (ADF-H) domain-containing family drives the formation of lamellipodia and filopodia at the leading edge of growth cones for axon guidance. However, the precise localization and function of ADF-H domain-containing proteins involved in axon extension and retraction remain unclear. We have previously shown that transcripts and proteins of coactosin-like protein 1 (COTL1), an ADF-H domain-containing protein, are observed in neurites and axons in chick embryos. Coactosin overexpression analysis revealed that this protein was localized to axonal growth cones and involved in axon extension in the midbrain. We further examined the specific distribution of coactosin and cofilin within the growth cone using superresolution microscopy, structured illumination microscopy, which overcomes the optical diffraction limitation and is suitable to the analysis of cellular dynamic movements. We found that coactosin was tightly associated with F-actin bundles at the growth cones and that coactosin overexpression promoted the expansion of lamellipodia and extension of growth cones. Coactosin knockdown in oculomotor neurons resulted in an increase in the levels of the inactive, phosphorylated form of cofilin and dysregulation of actin polymerization and axonal elongation, which suggests that coactosin promoted axonal growth in a cofilin-dependent manner. Indeed, the application of a dominant-negative form of LIMK1, a downstream effector of GTPases, reversed the effect of coactosin knockdown on axonal growth by enhancing cofilin activity. Combined, our results indicate that coactosin functions promote the assembly of protrusive actin filament arrays at the leading edge for growth cone motility.

    DOI: 10.3389/fcell.2021.660349

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  • Single-Cell Visualization Deep in Brain Structures by Gene Transfer 査読

    Sayaka Sugiyama, Junko Sugi, Tomoya Iijima, Xubin Hou

    Frontiers in Neural Circuits   14   2020年11月

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    担当区分:筆頭著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Media SA  

    DOI: 10.3389/fncir.2020.586043

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  • Reciprocal connectivity between secondary auditory cortical field and amygdala in mice. 査読 国際誌

    Tsukano H, Hou X, Horie M, Kitaura H, Nishio N, Hishida R, Takahashi K, Kakita A, Takebayashi H, Sugiyama S, Shibuki K

    Scientific reports   9 ( 1 )   19610 - 19610   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-019-56092-9

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  • Experience-dependent transcriptional regulation in juvenile brain development. 査読

    Sakai A, Sugiyama S

    Development, growth & differentiation   60 ( 8 )   473 - 482   2018年10月

  • Roles of CSGalNAcT1, a key enzyme in regulation of CS synthesis, in neuronal regeneration and plasticity. 査読 国際誌

    Michihiro Igarashi, Kosei Takeuchi, Sayaka Sugiyama

    Neurochemistry international   119   77 - 83   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Chondroitin sulfate (CS) is a sulfated glycosaminoglycan composed of a long chain of repeating disaccharide units that are attached to core proteins, resulting in CS proteoglycans (CSPGs). In the mature brain, CS is concentrated in perineuronal nets (PNNs), which are extracellular structures that surround synapses and regulate synaptic plasticity. In addition, CS is rapidly synthesized after CNS injury to create a physical and chemical barrier that inhibits axon growth. Most previous studies used a bacterial CS-degrading enzyme to investigate the physiological roles of CS. Recent studies have shown that CS is synthesized by more than 15 enzymes, all of which have been characterized in vitro. Here we focus on one of those enzymes, CSGalNAcT1 (T1). We produced T1 knockout mice (KO), which show extensive axon regeneration following spinal cord injury, as well as the loss of onset of ocular dominance plasticity. These results from T1KO mice suggest important roles for extracellular CS in the brain regarding neuronal plasticity and axon regeneration.

    DOI: 10.1016/j.neuint.2017.10.001

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  • Chondroitin Sulfate Is Required for Onset and Offset of Critical Period Plasticity in Visual Cortex 査読

    Xubin Hou, Nozomu Yoshioka, Hiroaki Tsukano, Akiko Sakai, Shinji Miyata, Yumi Watanabe, Yuchio Yanagawa, Kenji Sakimura, Kosei Takeuchi, Hiroshi Kitagawa, Takao K. Hensch, Katsuei Shibuki, Michihiro Igarashi, Sayaka Sugiyama

    SCIENTIFIC REPORTS   7 ( 1 )   12646   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Ocular dominance plasticity is easily observed during the critical period in early postnatal life. Chondroitin sulfate (CS) is the most abundant component in extracellular structures called perineuronal nets (PNNs), which surround parvalbumin-expressing interneurons (PV-cells). CS accumulates in PNNs at the critical period, but its function in earlier life is unclear. Here, we show that initiation of ocular dominance plasticity was impaired with reduced CS, using mice lacking a key CS-synthesizing enzyme, CSGalNAcT1. Two-photon in vivo imaging showed a weaker visual response of PV-cells with reduced CS compared to wild-type mice. Plasticity onset was restored by a homeoprotein Otx2, which binds the major CS-proteoglycan aggrecan and promotes its further expression. Continuous CS accumulation together with Otx2 contributed bidirectionally to both onset and offset of plasticity, and was substituted by diazepam, which enhances GABA function. Therefore, CS and Otx2 may act as common inducers of both onset and offset of the critical period by promoting PV-cell function throughout the lifetime.

    DOI: 10.1038/s41598-017-04007-x

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  • Genome-Wide Target Analyses of Otx2 Homeoprotein in Postnatal Cortex 査読

    Akiko Sakai, Ryuichiro Nakato, Yiwei Ling, Xubin Hou, Norikazu Hara, Tomoya Iijima, Yuchio Yanagawa, Ryozo Kuwano, Shujiro Okuda, Katsuhiko Shirahige, Sayaka Sugiyama

    FRONTIERS IN NEUROSCIENCE   11   2017年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS MEDIA SA  

    Juvenile brain has a unique time window, or critical period, in which neuronal circuits are remodeled by experience. Mounting evidence indicates the importance of neuronal circuit rewiring in various neurodevelopmental disorders of human cognition. We previously showed that Otx2 homeoprotein, essential for brain formation, is recaptured during postnatal maturation of parvalbumin positive interneurons (PV cells) to activate the critical period in mouse visual cortex. Cortical Otx2 is the only interneuron-enriched transcription factor known to regulate the critical period, but its downstream targets remain unknown. Here, we used ChIP-seq (chromatin immunoprecipitation sequencing) to identify genome-wide binding sites of Otx2 in juvenile mouse cortex, and interneuron-specific RNA-seq to explore the Otx2-dependent transcriptome. Otx2-bound genes were associated with human diseases such as schizophrenia as well as critical periods. Of these genes, expression of neuronal factors involved in transcription, signal transduction and mitochondria' function was moderately and broadly affected in Otx2-deficient interneurons. In contrast to reported binding sites in the embryo, genes encoding potassium ion transporters such as K(v)3.1 had juvenile cortex-specific binding sites, suggesting that Otx2 is involved in regulating fast-spiking properties during PV cell maturation. Moreover, transcripts of oxidative resistance-1 (Oxr1), whose promoter has Otx2 binding sites, were markedly downregulated in Otx2 deficient interneurons. Therefore, an important role of Otx2 may be to protect the cells from the increased oxidative stress in fast-spiking PV cells. Our results suggest that coordinated expression of Otx2 targets promotes PV cell maturation and maintains its function in neuronal plasticity and disease.

    DOI: 10.3389/fnins.2017.00307

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  • Associative-memory representations emerge as shared spatial patterns of theta activity spanning the primate temporal cortex 査読

    Kiyoshi Nakahara, Ken Adachi, Keisuke Kawasaki, Takeshi Matsuo, Hirohito Sawahata, Kei Majima, Masaki Takeda, Sayaka Sugiyama, Ryota Nakata, Atsuhiko Iijima, Hisashi Tanigawa, Takafumi Suzuki, Yukiyasu Kamitani, Isao Hasegawa

    NATURE COMMUNICATIONS   7   11827   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Highly localized neuronal spikes in primate temporal cortex can encode associative memory; however, whether memory formation involves area-wide reorganization of ensemble activity, which often accompanies rhythmicity, or just local microcircuit-level plasticity, remains elusive. Using high-density electrocorticography, we capture local-field potentials spanning the monkey temporal lobes, and show that the visual pair-association (PA) memory is encoded in spatial patterns of theta activity in areas TE, 36, and, partially, in the parahippocampal cortex, but not in the entorhinal cortex. The theta patterns elicited by learned paired associates are distinct between pairs, but similar within pairs. This pattern similarity, emerging through novel PA learning, allows a machine-learning decoder trained on theta patterns elicited by a particular visual item to correctly predict the identity of those elicited by its paired associate. Our results suggest that the formation and sharing of widespread cortical theta patterns via learning-induced reorganization are involved in the mechanisms of associative memory representation.

    DOI: 10.1038/ncomms11827

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  • Coactosin accelerates cell dynamism by promoting actin polymerization 査読

    Xubin Hou, Tatsuya Katahira, Kazumasa Ohashi, Kensaku Mizuno, Sayaka Sugiyama, Harukazu Nakamura

    DEVELOPMENTAL BIOLOGY   379 ( 1 )   53 - 63   2013年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    During development, cells dynamically move or extend their processes, which are achieved by actin dynamics. In the present study, we paid attention to Coactosin, an actin binding protein, and studied its role in actin dynamics. Coactosin was associated with actin and Capping protein in neural crest cells and N1E-115 neuroblastoma cells. Accumulation of Coactosin to cellular processes and its association with actin filaments prompted us to reveal the effect of Coactosin on cell migration. Coactosin overexpression induced cellular processes in cultured neural crest cells. In contrast, knock-down of Coactosin resulted in disruption of actin polymerization and of neural crest cell migration. Importantly, Coactosin was recruited to lamellipodia and filopodia in response to Rac signaling, and mutated Coactosin that cannot bind to F-actin did not react to Rac signaling, nor support neural crest cell migration. It was also shown that deprivation of Rac signaling from neural crest cells by dominant negative Rac1 (DN-Rac1) interfered with neural crest cell migration, and that co-transfection of DN-Rac1 and Coactosin restored neural crest cell migration. From these results we have concluded that Coactosin functions downstream of Rac signaling and that it is involved in neurite extension and neural crest cell migration by actively participating in actin polymerization. (C) 2013 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ydbio.2013.04.006

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  • Otx2 Binding to Perineuronal Nets Persistently Regulates Plasticity in the Mature Visual Cortex 査読

    Marine Beurdeley, Julien Spatazza, Henry H. C. Lee, Sayaka Sugiyama, Clemence Bernard, Ariel A. Di Nardo, Takao K. Hensch, Alain Prochiantz

    JOURNAL OF NEUROSCIENCE   32 ( 27 )   9429 - 9437   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC NEUROSCIENCE  

    Specific transfer of (orthodenticle homeobox 2) Otx2 homeoprotein into GABAergic interneurons expressing parvalbumin (PV) is necessary and sufficient to open, then close, a critical period (CP) of plasticity in the developing mouse visual cortex. The accumulation of endogenous Otx2 in PV cells suggests the presence of specific Otx2 binding sites. Here, we find that perineuronal nets (PNNs) on the surfaces of PV cells permit the specific, constitutive capture of Otx2. We identify a 15 aa domain containing an arginine-lysine doublet(RK peptide) within Otx2, bearing prototypic traits of a glycosaminoglycan (GAG) binding sequence that mediates Otx2 binding to PNNs, and specifically to chondroitin sulfate D and E, with high affinity. Accordingly, PNN hydrolysis by chondroitinase ABC reduces the amount of endogenous Otx2 in PV cells. Direct infusion of RK peptide similarly disrupts endogenous Otx2 localization to PV cells, reduces PV and PNN expression, and reopens plasticity in adult mice. The closure of one eye during this transient window reduces cortical acuity and is specific to the RK motif, as an Alanine-Alanine variant or a scrambled peptide fails to reactivate plasticity. Conversely, this transient reopening of plasticity in the adult restores binocular vision in amblyopic mice. Thus, one function of PNNs is to facilitate the persistent internalization of Otx2 by PV cells to maintain CP closure. The pharmacological use of the Otx2 GAG binding domain offers a novel, potent therapeutic tool with which to restore cortical plasticity in the mature brain.

    DOI: 10.1523/JNEUROSCI.0394-12.2012

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  • From brain formation to plasticity: Insights on Otx2 homeoprotein 査読

    Sayaka Sugiyama, Alain Prochiantz, Takao K. Hensch

    DEVELOPMENT GROWTH & DIFFERENTIATION   51 ( 3 )   369 - 377   2009年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    The shaping of neuronal circuits is essential during postnatal brain development. A window of neuronal remodeling by sensory experience typically occurs during a unique time in early life. The many types of behavior and perception, like human language, birdsong, hearing and vision are refined by experience during these distinct 'critical periods'. The onset of critical periods for vision is delayed in animals that remain in complete darkness from birth. It is then predicted that a 'messenger' within the visual pathway signals the amount of sensory experience that has occurred. Our recent results indicate that Otx2 homeoprotein, an essential morphogen for embryonic head formation, is reused later in life as this 'messenger' for critical period plasticity. The homeoprotein is stimulated by visual experience to propagate into the visual cortex, where it is internalized by GABAergic interneurons, especially Parvalbumin-positive cells (PV-cells). Otx2 promotes the maturation of PV-cells, consequently activating critical period onset in the visual cortex. Here, we discuss recent data that are beginning to illuminate the physiological function of non-cell autonomous homeoproteins, as well as the restriction of their transfer to PV-cells in vivo.

    DOI: 10.1111/j.1440-169X.2009.01093.x

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  • Clonal and widespread gene transfer by proviral electroporation for analysis of brain laminar formation 査読

    Sayaka Sugiyama, Harukazu Nakamura

    Electroporation and Sonoporation in Developmental Biology   117 - 127   2009年

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer Japan  

    An essential approach to understanding the mechanisms of development is to alter a gene function/expression. In vivo electroporation has been adapted as one such technique (Muramatsu et al., 1997). It is a very useful tool to achieve a gain- and loss-of-function (by using RNAi or morpholinos) of a gene of interest (Funahashi et al., 1999
    Fukuchi-Shimogori and Grove, 2001
    Kos et al., 2001
    Katahira and Nakamura, 2003
    Sugiyama and Nakamura, 2003). The technique has allowed the altering of gene expression temporally and spatially. Pulse-labeling technique is an approach to manipulate a specific cell population temporally, depending on its birthday, as this chapter describes. This technique is more advantageous over the BrdU application, as it can reveal cell lineage
    it also has the ability to manipulate a gain- and loss-of-function into specific precursor cells (Tabata and Nakajima, 2001
    Sugiyama and Nakamura, 2003
    Huber et al., 2008). In spatial terms, widespread gene transfer by electroporation has provided an efficient way to unveil a new gene function on a given tissue (Nakamura and Funahashi, 2001). On the other hand, the spatial precision of gene transfer also has been addressed as each individual cell responds differently to gene expression. Thus, there has been increasing efforts to improve electrodes and create new techniques such as single-cell electroporation (Haas et al., 2001
    Kitamura et al., 2008). © 2009 Springer Japan.

    DOI: 10.1007/978-4-431-09427-2_12

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  • Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity 査読

    Sayaka Sugiyama, Ariel A. Di Nardo, Shinichi Aizawa, Isao Matsuo, Michel Volovitch, Alain Prochiantz, Takao K. Hensch

    CELL   134 ( 3 )   508 - 520   2008年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

    Neural circuits are shaped by experience in early postnatal life. Distinct GABAergic connections within visual cortex determine the timing of the critical period for rewiring ocular dominance to establish visual acuity. We find that maturation of the parvalbumin (PV)-cell network that controls plasticity onset is regulated by a selective re-expression of the embryonic Otx2 homeoprotein. Visual experience promoted the accumulation of non-cell-autonomous Otx2 in PV-cells, and cortical infusion of exogenous Otx2 accelerated both PV-cell development and critical period timing. Conversely, conditional removal of Otx2 from non-PV cells or from the visual pathway abolished plasticity. Thus, the experience-dependent transfer of a homeoprotein may establish the physiological milieu for postnatal plasticity of a neural circuit.

    DOI: 10.1016/j.cell.2008.05.054

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  • Polarity and laminar formation of the optic tectum in relation to retinal projection 査読

    H Nakamura, S Sugiyama

    JOURNAL OF NEUROBIOLOGY   59 ( 1 )   48 - 56   2004年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JOHN WILEY & SONS INC  

    The mes-metencephalic boundary (isthmus) works as an organizer for the tectum, and the organizing molecule may be Fgf8. The region where Otx2, En1, and Pax2 are expressed overlappingly may differentiate into the mesencephalon. The di-mesencephalic and mes-metencephalic boundaries are determined by repressive interaction of Pax6 and En1/Pax2 and of Otx2 and Gbx2, respectively. The optic tectum is a visual center in lower vertebrates. The tectum and the retina should be regionalized and be positionally specialized for the proper retinotopic projection. Gradient of En2 plays a crucial role in rostrocaudal polarity formation of the tectum. En2 confers caudal characteristics of the retina by inducing ephrinA2 and A5, which are the repellant molecules for the growth cones of temporal retinal ganglion cells. Grg4 antagonizes the isthmus-related genes, and is involved in the formation of di-mesencephalic boundary and tectal polarity formation at an early phase of development. Then, Grg4 plays a role in tectal laminar formation by controlling the migration pathway. Migration pathway of tectal postmitotic cells changes after E5. The late migratory cells split the early migratory neurons to form laminae h-j of SGFS. Grg4 is expressed in the ventricular layer after E5, and forces postmitotic cells to follow the late migratory pathway, though retinal fibers terminate at laminae a-f of SGFS. Misexpression of Grg4 disrupts the lamina g, and in such tecta retinal arbors invade deep into the tectal layer, indicating that lamina g is a nonpermissive lamina for the retinal arbors. (C) 2004 Wiley Periodicals, Inc.

    DOI: 10.1002/neu.10339

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  • The role of Grg4 in tectal laminar formation 査読

    S Sugiyama, H Nakamura

    DEVELOPMENT   130 ( 3 )   451 - 462   2003年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    Mature chick optic tecta consist of 16 laminae and receive retinal fiber projections in a precise retinotopic manner. Retinal axons arborize in laminae a-f of the SGFS, but do not cross the border between lamina f and g. In order to elucidate molecular mechanisms of tectal laminar formation, we first looked at the migration of tectal postmitotic cells. We found that the migration pattern of postmitotic cells changes around E5 and that late migratory cells intervened laminae that were formed by early migratory cells. The coincident appearance of Grg4 expression in the tectal ventricular layer and the change in migration pattern suggested an important role for Grg4. 451 Clonal misexpression of Grg4 resulted in cells migrating to laminae h-j of the SGFS. Massive misexpression of Grg4 resulted in disruption of laminae that were formed by early migratory cells, in particular lamina g of the SGFS. Application of Grg4 morpholino antisense oligonucleotide or the misexpression of a dominant-negative form of Grg4 exerted the opposite effect. We concluded that Grg4 may direct tectal postmitotic cells to follow a late migratory pathway.

    DOI: 10.1242/dev.00232

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  • Antagonizing activity of chick Grg4 against tectum-organizing activity 査読

    S Sugiyama, J Funahashi, H Nakamura

    DEVELOPMENTAL BIOLOGY   221 ( 1 )   168 - 180   2000年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    Alar plate of chick mesencephalon differentiates into the optic tectum. It has been shown that factors expressed in the mes-metencephalic boundary induce the tectum and give positional specificity. Chick Grg4 is expressed at first in the anterior neural fold. The expression localizes from the posterior diencephalon to the mesencephalon by stage 10. To investigate the function of Grg4 in mesencephalic development, Grg4 overexpression was carried out by in ovo electroporation. After Grg4 overexpression, expression of En-2, Pax5, Fgf8, and EphrinA2 was repressed, and Pax6 was upregulated in the mesencephalic region. Grg4 overexpression caused the morphological change; mesencephalic swelling became smaller and the di-mesencephalic boundary shifted posteriorly, that is, the anterior limit of tectum shifted posteriorly. Importantly, cotransfection of Grg4 with Pax5 canceled the tectum-inducing activity of Pax5. These results suggest that Grg4 works as an antagonist against tectum-organizing activity. It was also shown that transfected N-terminal domains of Grg4 induced En-2 expression. Since N-terminal domains were transported to the nucleus in the neuroepithelium, they could act as dominant negative for endogenous Grg4. These results indicate that Grg4 has repressing activity against the organizing molecules and suggest that Grg4 plays important roles in formation of anterior tectal boundary and polarity. (C) 2000 Academic Press.

    DOI: 10.1006/dbio.2000.9643

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  • Interaction between Otx2 and Gbx2 defines the organizing center for the optic tectum 査読

    T Katahira, T Sato, S Sugiyama, T Okafuji, Araki, I, J Funahashi, H Nakamura

    MECHANISMS OF DEVELOPMENT   91 ( 1-2 )   43 - 52   2000年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Otx2 is expressed in the mesencephalon and prosencephalon, and Gbx2 is expressed in the rhombencephalon around stage 10. Loss-of-function studies of these genes in mice have revealed that Otx2 is indispensable for the development of the anterior brain segment, and that Gbx2 is required for the development of the isthmus. We carried out gain-of-function experiments of these genes in chick embryos with a newly developed gene transfer system, in ovo electroporation. When Otx2 was ectopically expressed caudally beyond the midbrain-hindbrain boundary (MHB), the alar plate of the metencephalon differentiated into the optic tectum instead of differentiating into the cerebellum. On the other hand, when Gbx2 was ectopically expressed at the mesencephalon, the caudal limit of the tectum shifted rostrally. We looked at the effects of misexpression on the isthmus- and tectum-related molecules. Otx2 and Gbx2 interacted to repress each other's expression. Ectopic Otx2 and Gbx2 repressed endogenous expression of Fgf8 in the isthmus, but induced Fgf8 expression at the interface between Otx2 and Gbx2 expression. Thus, it is suggested that interaction between Otx2 and Gbx2 determines the site of Fgf8 expression and the posterior limit of the tectum. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

    DOI: 10.1016/S0925-4773(99)00262-2

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  • Apical ectodermal ridge induction by the transplantation of En-1-overexpressing ectoderm in chick limb bud 査読

    M Tanaka, Y Shigetani, S Sugiyama, K Tamura, H Nakamura, H Ide

    DEVELOPMENT GROWTH & DIFFERENTIATION   40 ( 4 )   423 - 429   1998年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL SCIENCE  

    In the early chick embryo, the dorsal-ventral (DV) boundary organizes the apical ectodermal ridge (AER) structure in the limb bud field. Here it is reported that Engrailed-1 (En-l), a homolog of the Drosophila segment polarity gene engrailed expressed in the ventral limb ectoderm, participates in AER formation at the DV boundary of the limb bud. Restricted ectopic expression of En-1 in the dorsal side of the limb bud by transplantation of En-1-overexpressing ectoderm induces ectopic AER at the boundary of En-1-positive and -negative cells. The results suggest that En-1 is involved in AER formation at the DV boundary of the limb bud.

    DOI: 10.1046/j.1440-169X.1998.t01-2-00007.x

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  • Crossregulation between En-2 and Wnt-1 in chick tectal development 査読

    S Sugiyama, J Funahashi, J Kitajewski, H Nakamura

    DEVELOPMENT GROWTH & DIFFERENTIATION   40 ( 2 )   157 - 166   1998年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL SCIENCE  

    En-1, En-2 and Wnt-1 are proposed to be essential signals for the development of the optic tectum in chick embryos. Drosophila engrailed and wingless, homologs of En (En-1 and En-2) and Wnt-1, respectively, have been shown to crossregulate each other. In the present paper, it is reported that crossregulation between En-2 and Wnt-1 is preserved in the development of the chick optic tectum. When En-2 is overexpressed by the replication competent retroviral vector, Wnt-1 is expressed ectopically at the dorsal midline of the diencephalon. When Wnt-1 is introduced extrinsically either by ectopic transplantation of mesencephalon, or by implantation of Wnt-1 producing cells, En-2 is induced ectopically at the dorsal midline of the tel-diencephalic border. Thus, ectopic expression of En-2 and Wnt-1 leads to crossregulation of each other in the chick brain. As diencephalon transdifferentiates into the optic tectum by an appropriate signal, the crossregulation of En-2 and Wnt-1 in the diencephalon may mimic the relationship required for early development in the tectum.

    DOI: 10.1046/j.1440-169X.1998.00005.x

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書籍等出版物

  • 分子脳科学: 分子から脳機能と心に迫る (DOJIN BIOSCIENCE)

    杉山清佳, 三品 昌美( 担当: 分担執筆 ,  範囲: 第23章 脳発達の臨界期)

    化学同人  2015年4月  ( ISBN:4759815198

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    総ページ数:291  

    ASIN

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  • Electroporation and Sonoporation in Developmental Bilogy

    Sugiyama S, Nakamura H, Nakamura Harukazu( 担当: 分担執筆 ,  範囲: Clonal and widespread gene transfer by proviral electroporation for analysis of brain laminar formation)

    Springer  2009年4月  ( ISBN:4431094261

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    総ページ数:364  

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MISC

  • 弱視の臨界期に関する最近の知見

    杉山清佳, 侯旭濱

    眼科   61 ( 1 )   2019年1月

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  • ドーパミン作動性細胞に発現するOtx2の恐怖記憶における役割

    飯島 友也, 杉山 清佳

    新潟医学会雑誌   132 ( 2 )   49 - 59   2018年2月

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    記述言語:日本語   出版者・発行元:新潟医学会  

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  • 臨界期の機構 : 子どもの脳の柔軟性を紐解く (特集 大脳皮質 : 成り立ちから機能へ)

    酒井 晶子, 杉山 清佳

    生体の科学   68 ( 1 )   54 - 58   2017年1月

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    記述言語:日本語   出版者・発行元:金原一郎記念医学医療振興財団 ; 1949-  

    CiNii Article

    CiNii Books

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  • 臨界期

    杉山清佳

    脳科学辞典 DOI:10.14931/bsd.6426   2015年

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  • 【弱視研究の最先端】 弱視の臨界期メカニズム ホメオ蛋白質と糖鎖の新しい役割

    杉山 清佳

    神経眼科   29 ( 4 )   396 - 403   2012年12月

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    記述言語:日本語   出版者・発行元:日本神経眼科学会  

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  • 「脳の柔軟性」を生む分子メカニズム‐Otx2ホメオ蛋白質の新しい役割‐

    杉山清佳

    新潟医学会雑誌   125 ( 4 )   175 - 179   2011年4月

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    記述言語:日本語   出版者・発行元:新潟医学会  

    CiNii Article

    CiNii Books

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  • 神経細胞の移動・投射・層構造 峡部オーガナイザーと小脳・視蓋形成 (神経の分化,回路形成,機能発現) -- (神経の発生と分化)

    仲村春和, 杉山清佳, 佐藤達也

    蛋白質核酸酵素   53 ( 4 )   373 - 378   2008年3月

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    記述言語:日本語   出版者・発行元:共立出版  

    CiNii Article

    CiNii Books

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  • 中脳領域の特異化 (特集 脳の発達に関与する分子機構)

    杉山清佳, 仲村春和

    生体の科学   52 ( 3 )   177 - 181   2001年5月

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    記述言語:日本語   出版者・発行元:金原一郎記念医学医療振興財団  

    DOI: 10.11477/mf.2425902265

    CiNii Article

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  • 【小児神経学の最近の話題】 神経系の分化と発達 脳の形態形成

    杉山 清佳, 仲村 春和

    Clinical Neuroscience   17 ( 3 )   254 - 257   1999年3月

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    記述言語:日本語   出版者・発行元:(株)中外医学社  

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  • ニワトリ胚中脳後側に強く発現する遺伝子Wnt-1とEnの相互作用

    杉山 清佳

    加齢医学研究所雑誌   48 ( 2 )   115 - 115   1997年3月

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    記述言語:日本語   出版者・発行元:東北大学加齢医学研究所  

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  • ニワトリ胚中脳の吻尾極性に関わる遺伝子カスケードの解析

    舟橋 淳一, 杉山 清佳, 原 知永, 仲村 春和

    日本分子生物学会年会プログラム・講演要旨集   19   673 - 673   1996年8月

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講演・口頭発表等

  • 弱視の臨界期の分子メカニズム

    杉山清佳

    第122回日本眼科学会総会  2018年 

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  • Dendritic organization of cortical interneurons in anticipation of postnatal plasticity

    Sayaka Sugiyama

    The 41th Annual Meeting of the Japan Neuroscience Society  2018年 

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    会議種別:シンポジウム・ワークショップ パネル(公募)  

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  • 視覚経験による個性的回路の創出機構

    杉山清佳

    次世代脳プロジェクト 冬のシンポジウム  2017年 

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  • Transcriptional regulation of homeoprotein in postnatal cortical plasticity.

    Sayaka Sugiyama

    The 40th Annual Meeting of the Japan Neuroscience Society  2017年 

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  • Experience-dependent maturation of inhibitory circuits in ocular dominance plasticity.

    Sayaka Sugiyama

    The 39th Annual Meeting of the Japan Neuroscience Society.  2016年 

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  • Ocular dominance plasticity regulated by Otx2-inducible molecules.

    Sayaka Sugiyama

    The 58th Annual Meeting of the Japan Society for Neurochemistry  2015年 

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  • Inhibitory maturation regulates the critical period plasticity for binocular vision.

    Sayaka Sugiyama

    The 120th Annual Meeting of the Japanese Association of Anatomists  2015年 

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  • コンドロイチン硫酸による量依存的な臨界期制御

    杉山清佳

    第38回日本分子生物学会年会  2015年 

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  • コンドロイチン硫酸とホメオ蛋白質による臨界期制御

    杉山清佳

    第87回日本生化学会大会  2014年 

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  • 臨界期のメカニズム-経験依存の回路形成-

    杉山清佳

    第38回峠の会(形態学セミナー)  2014年 

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  • コンドロイチン硫酸による抑制性ニューロンの機能発達と臨界期制御

    杉山清佳

    第57回日本神経化学会大会  2014年 

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  • 臨界期の時期を制御する分子たち

    杉山清佳

    第18回視覚科学フォーラム 特別企画シンポジウム  2014年 

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  • 臨界期の分子メカニズム-モデル動物からの知見-

    杉山清佳

    第69回日本弱視斜視学会総会  2013年 

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  • 臨界期におけるホメオ蛋白質の新しい役割

    杉山清佳

    東北大学脳センターシンポジューム  2013年 

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  • Otx2 binding to sugar chains regulates plasticity in mouse visual cortex.

    Sayaka Sugiyama

    NIG workshop: Circuit construction in the mammalian cerebral cortex  2012年 

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  • Interaction of Otx2 and glycosaminoglycan regulates cortical plasticity for binocular vision.

    Sayaka Sugiyama

    The 35th Annual Meeting of the Japan Neuroscience Society  2012年 

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受賞

  • 女性研究者サイエンスグラント賞

    2018年7月   資生堂  

    杉山清佳

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  • 第一回女性研究者賞

    2013年11月   ソロプチミスト日本財団  

    杉山清佳

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共同研究・競争的資金等の研究

  • 経験による大脳抑制性ニューロンの形態形成と機能分化メカニズムの解析

    2019年4月 - 2023年3月

    科学研究費補助金  基盤研究B 

    杉山清佳

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    担当区分:研究代表者  資金種別:競争的資金

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  • ホメオ蛋白質の移動原理と新たな効能の探究

    2018年4月 - 2020年3月

    科学研究費補助金  挑戦的研究(萌芽) 

    杉山清佳

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    担当区分:研究代表者  資金種別:競争的資金

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  • 視覚経験による個性的回路の創出機構

    2017年4月 - 2019年3月

    科学研究費補助金  新学術領域研究 

    杉山清佳

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    担当区分:研究代表者  資金種別:競争的資金

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  • ホメオ蛋白質による臨界期の分子メカニズムの解析

    2014年4月 - 2017年3月

    科学研究費補助金  基盤研究C 

    杉山清佳

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    担当区分:研究代表者  資金種別:競争的資金

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  • アクチン再構築による臨界期制御メカニズムの解析

    2014年4月 - 2016年3月

    科学研究費補助金  外国人特別研究員奨励費 

    杉山清佳

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    担当区分:研究代表者  資金種別:競争的資金

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  • 経験が脳の発達を促すメカニズム

    2011年3月 - 2014年3月

    日本学術振興会  最先端次世代研究開発支援プログラム 

    杉山清佳

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    担当区分:研究代表者  資金種別:競争的資金

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  • 臨界期を制御するホメオ蛋白質の新しい役割

    2010年10月 - 2011年3月

    科学技術振興機構  さきがけ 

    杉山清佳

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    担当区分:研究代表者  資金種別:競争的資金

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担当経験のある授業科目(researchmap)

  • 人体の構造と機能I(解剖総論・肉眼解剖学)

    機関名:新潟大学

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  • 人体の構造と機能I(生理学)

    機関名:新潟大学

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  • 先端医科学研究概説

    機関名:新潟大学

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  • 人体の構造と機能II(神経解剖学・実習)

    機関名:新潟大学

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  • 医学研究実習

    機関名:新潟大学

     詳細を見る

  • 細胞生物学

    機関名:新潟大学

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  • 脳と心の医科学

    機関名:新潟大学

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  • 人体の構造と機能II(生理学・実習)(新潟大学)

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担当経験のある授業科目

  • 先端医科学研究概説

    2022年
    -
    現在
    機関名:新潟大学

  • 生理学実習

    2021年
    -
    現在
    機関名:新潟大学

  • 人体の構造と機能II(生理学)

    2021年
    -
    現在
    機関名:新潟大学