Updated on 2023/02/05

写真a

 
IDA Takako
 
Organization
Academic Assembly Institute of Medicine and Dentistry SHIGAKU KEIRETU Assistant Professor
Faculty of Dentistry School of Dentistry Assistant Professor
Graduate School of Medical and Dental Sciences Oral Life Science Oral Health Science Assistant Professor
Title
Assistant Professor
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Degree

  • 歯学(博士) ( 2016.3   新潟大学 )

Research Interests

  • バイオフィルム

  • 歯根膜

  • コラーゲン

Research Areas

  • Life Science / Bacteriology

  • Life Science / Prosthodontics

Research History

  • Niigata University   School of Dentistry, Faculty of Dentistry   Assistant Professor

    2021.5

  • Niigata University   Oral Health Science, Oral Life Science, Graduate School of Medical and Dental Sciences   Assistant Professor

    2021.5

  • Niigata University   Institute of Medicine and Dentistry, Academic Assembly   Assistant Professor

    2021.5

  • Niigata University   Graduate School of Medical and Dental Sciences   Specially Appointed Assistant Professor

    2020.4 - 2021.4

  • Niigata University   Institute of Medicine and Dentistry, Academic Assembly   Specially Appointed Assistant Professor

    2020.4 - 2021.4

  • Niigata University   Graduate School of Medical and Dental Sciences   Specially Appointed Assistant Professor

    2017.10 - 2018.3

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Professional Memberships

  • 日本バイオフィルム学会

    2021.7

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  • The Japanese Society of Conservative Dentistry

    2020.11

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  • The International Association for Dental Research

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  • JAPAN PROSTHODONTIC SOCIETY

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Papers

  • Efficacy of Combining an Extraoral High-Volume Evacuator with Preprocedural Mouth Rinsing in Reducing Aerosol Contamination Produced by Ultrasonic Scaling. International journal

    Shoji Takenaka, Maki Sotozono, Asaka Yashiro, Rui Saito, Niraya Kornsombut, Traithawit Naksagoon, Ryoko Nagata, Takako Ida, Naoki Edanami, Yuichiro Noiri

    International journal of environmental research and public health   19 ( 10 )   2022.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    The coronavirus disease pandemic has afforded dental professionals an opportunity to reconsider infection control during treatment. We investigated the efficacy of combining extraoral high-volume evacuators (eHVEs) with preprocedural mouth rinsing in reducing aerosol contamination by ultrasonic scalers. A double-masked, two-group, crossover randomized clinical trial was conducted over eight weeks. A total of 10 healthy subjects were divided into two groups; they received 0.5% povidone-iodine (PI), essential oil (EO), or water as preprocedural rinse. Aerosols produced during ultrasonic scaling were collected from the chest area (PC), dentist's mask, dentist's chest area (DC), bracket table, and assistant's area. Bacterial contamination was assessed using colony counting and adenosine triphosphate assays. With the eHVE 10 cm away from the mouth, bacterial contamination by aerosols was negligible. With the eHVE 20 cm away, more dental aerosols containing bacteria were detected at the DC and PC. Mouth rinsing decreased viable bacterial count by 31-38% (PI) and 22-33% (EO), compared with no rinsing. The eHVE prevents bacterial contamination when close to the patient's mouth. Preprocedural mouth rinsing can reduce bacterial contamination where the eHVE is positioned away from the mouth, depending on the procedure. Combining an eHVE with preprocedural mouth rinsing can reduce bacterial contamination in dental offices.

    DOI: 10.3390/ijerph19106048

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  • Periodontal pathogens inhabit root caries lesions extending beyond the gingival margin: A next-generation sequencing analysis

    Takenaka S, Edanami n, Komatsu Y, Nagata R, Naksagoon T, Sotozono M, Ida T, Noiri Y

    Microorganisms   2021.11

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  • A Repeated State of Acidification Enhances the Anticariogenic Biofilm Activity of Glass Ionomer Cement Containing Fluoro-Zinc-Silicate Fillers Reviewed

    Naksagoon T, Takenaka S, Nagata R, Sotozono M, Ohsumi T, Ida T, Edanami N, Maeda T, Noiri Y

    Antibiotics   WEB   2021.8

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  • Effect of a novel glass ionomer cement containing fluoro-zinc-silicate fillers on biofilm formation and dentin ion incorporation. Reviewed International journal

    Taisuke Hasegawa, Shoji Takenaka, Tatsuya Ohsumi, Takako Ida, Hayato Ohshima, Yutaka Terao, Traithawit Naksagoon, Takeyasu Maeda, Yuichiro Noiri

    Clinical oral investigations   24 ( 2 )   963 - 970   2020.2

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    OBJECTIVES: This study is aimed at evaluating the effect of a new glass ionomer cement (GIC) containing fluoro-zinc-silicate fillers on biofilm formation and ion incorporation. MATERIALS AND METHODS: Streptococcus mutans biofilms were developed on two GIC materials: Caredyne Restore (CD) and Fuji VII (FJ); and hydroxyapatite (HA) for 24 h at 37 °C using a flow cell system. The morphological structure and bacterial viability were analyzed using a confocal laser scanning microscopy. Bacterial adhesion during the initial 2 h was also assessed by viable cell counting. To study the ion incorporation, restored cavities prepared on the root surfaces of human incisors were subjected to the elemental mapping of the zinc and fluoride ions in the GIC-dentin interface using a wavelength-dispersive X-ray spectroscopy electron probe microanalyzer. RESULTS: Morphological observations revealed that biofilm formation in the CD group was remarkably inhibited compared with the HA and FJ groups, exhibiting sparse, thinner biofilm clusters. The microorganisms adhering to the CD group were significantly inhibited, revealing 2.9 ± 0.4 for CD, 4.9 ± 0.2 for FJ, and 5.4 ± 0.4 log colony-forming units (CFU) for HA. The CD zinc ion incorporation depth was 72.2 ± 8.0 μm. The fluoride penetration of CD was three times deeper than that of FJ; this difference was statistically significant (p < 0.05). CONCLUSIONS: Enhanced by the incorporation of zinc and fluoride ions, the new GIC inhibited biofilm formation by interfering with bacterial adhesion. CLINICAL RELEVANCE: A novel GIC comprised of fluoro-zinc-silicate fillers may improve clinical outcomes, such as root caries and minimally invasive dentistry.

    DOI: 10.1007/s00784-019-02991-0

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  • Response to Letter to the Editor: Concerns on modeling postmenopausal osteoporosis in young female rats. Reviewed International journal

    Juan Marcelo Rosales Rocabado, Masaru Kaku, Kosuke Nozaki, Takako Ida, Megumi Kitami, Yujin Aoyagi, Katsumi Uoshima

    Journal of orthopaedic surgery and research   14 ( 1 )   451 - 451   2019.12

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  • 矯正的歯の移動時における歯根膜増殖期細胞の特性

    水越 優, 加来 賢, 北見 公平, 新井 萌生, 井田 貴子, 魚島 勝美, 齋藤 功

    新潟歯学会雑誌   49 ( 2 )   84 - 84   2019.12

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  • 矯正的歯の移動時のマウス歯根膜における増殖期細胞の局在と特性

    水越 優, 加来 賢, 北見 公平, 井田 貴子, 新井 萌生, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   78回   172 - 172   2019.11

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  • 【海外の基礎研究はインプラント治療をどう変えたか?:From Bench to Clinic】オッセオインテグレーションの獲得に関わる骨代謝とコラーゲン架橋 Reviewed

    加来 賢, 井田 貴子, 長澤 麻沙子, 魚島 勝美

    日本口腔インプラント学会誌   32 ( 2 )   108 - 115   2019.6

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    Language:Japanese   Publisher:(公社)日本口腔インプラント学会  

    デンタルインプラントにおける術前検査と埋入プロトコルが確立されつつある現在では,その長期予後をいかにして予測し,これを維持するかが新たな課題となっている.長らく歯科界でインプラント適用時の判断基準として使われてきた概念,すなわちインプラント埋入部位の骨量と骨密度を基にした分類は,インプラントの初期固定と骨の力学的支持能力を評価する際に効果的であることは明白である.しかしながら,整形外科領域で診断根拠として用いられる"骨質"は,骨の機械的強度に影響を及ぼす因子の中でも骨密度以外の要素を指し,時に歯科で使われる"骨質"とは全く異なる概念である.整形外科領域で使われる"骨質"の代表的な要素の一つであるコラーゲン架橋は,コラーゲン生合成の過程において細胞内外で生じる一連の翻訳後修飾の結果として形成される分子間架橋であり,その量や構成比が組織の機械的特性に寄与していることが知られている.また,近年の研究から,組織中のコラーゲン架橋は細胞活性を制御することが報告されており,骨組織中のコラーゲン架橋は組織の機械的強度に影響を及ぼすだけでなく,局所的な骨代謝回転を制御する因子としても機能していると考えられる.本総説では骨質の一要素でもあるコラーゲン架橋の新たな機能と,そのインプラント治療における臨床的意義および将来的な展望について紹介する.(著者抄録)

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  • オッセオインテグレーションの獲得に関わる骨代謝とコラーゲン架橋 Reviewed

    加来 賢, 井田貴子, 長澤麻沙子, 魚島勝美

    日本口腔インプラント学会誌   32 ( 2 )   26 - 32   2019

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  • A multi-factorial analysis of bone morphology and fracture strength of rat femur in response to ovariectomy. Reviewed International journal

    Juan Marcelo Rosales Rocabado, Masaru Kaku, Kosuke Nozaki, Takako Ida, Megumi Kitami, Yujin Aoyagi, Katsumi Uoshima

    Journal of orthopaedic surgery and research   13 ( 1 )   318 - 318   2018.12

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    BACKGROUND: Postmenopausal osteoporosis develops due to a deficiency of estrogen that causes a decrease in bone mass and changes in the macro- and micro-architectural structure of the bone, leading to the loss of mechanical strength and an increased risk of fracture. Although the assessment of bone mineral density (BMD) has been widely used as a gold standard for diagnostic screening of bone fracture risks, it accounts for only a part of the variation in bone fragility; thus, it is necessary to consider other determinants of bone strength. Therefore, we aimed to comprehensively evaluate the architectural changes of the bone that influence bone fracture strength, together with the different sensitivities of cortical and trabecular bone in response to ovariectomy (OVX). METHODS: Bone morphology parameters were separately analyzed both in cortical and in trabecular bones, at distal-metaphysis, and mid-diaphysis of OVX rat femurs. Three-point bending test was performed at mid-diaphysis of the femurs. Correlation of OVX-induced changes of morphological parameters with breaking force was analyzed using Pearson's correlation coefficient. RESULTS: OVX resulted in a decline in the bone volume of distal-metaphysis trabecular bone, but an increase in distal-metaphysis and mid-diaphysis cortical bone volume. Tissue mineral density (TMD) remained unchanged in both the trabecular and cortical bone of the distal metaphysis but decreased in cortical bone of the mid-diaphysis. The OVX significantly increased the breaking force at mid-diaphysis of the femurs. CONCLUSIONS: OVX decreased the trabecular bone volume of the distal-metaphysis and increased the cortical bone volume of the distal-metaphysis and mid-diaphysis. Despite the reduction in TMD and increased cortical porosity, bone fracture strength increased in the mid-diaphysis after OVX. These results indicate that analyzing a single factor, i.e., BMD, is not sufficient to predict the absolute fracture risk of the bone, as OVX-induced bone response vary, depending on the bone type and location. Our results strongly support the necessity of analyzing bone micro-architecture and site specificity to clarify the true etiology of osteoporosis in a clinical setting.

    DOI: 10.1186/s13018-018-1018-4

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  • Extracellular matrix with defective collagen cross-linking affects the differentiation of bone cells. Reviewed International journal

    Takako Ida, Masaru Kaku, Megumi Kitami, Masahiko Terajima, Juan Marcelo Rosales Rocabado, Yosuke Akiba, Masako Nagasawa, Mitsuo Yamauchi, Katsumi Uoshima

    PloS one   13 ( 9 )   e0204306   2018

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    Fibrillar type I collagen, the predominant organic component in bone, is stabilized by lysyl oxidase (LOX)-initiated covalent intermolecular cross-linking, an important determinant of bone quality. However, the impact of collagen cross-linking on the activity of bone cells and subsequent tissue remodeling is not well understood. In this study, we investigated the effect of collagen cross-linking on bone cellular activities employing a loss-of-function approach, using a potent LOX inhibitor, β-aminopropionitrile (BAPN). Osteoblastic cells (MC3T3-E1) were cultured for 2 weeks in the presence of 0-2 mM BAPN to obtain low cross-linked collagen matrices. The addition of BAPN to the cultures diminished collagen cross-links in a dose-dependent manner and, at 1 mM level, none of the major cross-links were detected without affecting collagen production. After the removal of cellular components from these cultures, MC3T3-E1, osteoclasts (RAW264.7), or mouse primary bone marrow-derived stromal cells (BMSCs) were seeded. MC3T3-E1 cells grown on low cross-link matrices showed increased alkaline phosphatase (ALP) activity. The number of multinucleate tartrate-resistant acid phosphatase (TRAP)-positive cells increased in RAW264.7 cells. Initial adhesion, proliferation, and ALP activity of BMSCs also increased. In the animal experiments, 4-week-old C57BL/6 mice were fed with BAPN-containing diet for 8 weeks. At this point, biochemical analysis of bone demonstrated that collagen cross-links decreased without affecting collagen content. Then, the diet was changed to a control diet to minimize the direct effect of BAPN. At 2 and 4 weeks after the change, histological samples were prepared. Histological examination of femur samples at 4 weeks showed a significant increase in the number of bone surface osteoblasts, while the bone volume and surface osteoclast numbers were not significantly affected. These results clearly demonstrated that the extent of collagen cross-linking of bone matrix affected the differentiation of bone cells, underscoring the importance of collagen cross-linking in the regulation of cell behaviors and tissue remodeling in bone. Characterization of collagen cross-linking in bone may be beneficial to obtain insight into not only bone mechanical property, but also bone cellular activities.

    DOI: 10.1371/journal.pone.0204306

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  • An ENU-induced splice site mutation of mouse Col1a1 causing recessive osteogenesis imperfecta and revealing a novel splicing rescue. Reviewed International journal

    Koichi Tabeta, Xin Du, Kei Arimatsu, Mai Yokoji, Naoki Takahashi, Norio Amizuka, Tomoka Hasegawa, Karine Crozat, Tomoki Maekawa, Sayuri Miyauchi, Yumi Matsuda, Takako Ida, Masaru Kaku, Kasper Hoebe, Kinji Ohno, Hiromasa Yoshie, Kazuhisa Yamazaki, Eva Marie Y Moresco, Bruce Beutler

    Scientific reports   7 ( 1 )   11717 - 11717   2017.9

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    GU-AG consensus sequences are used for intron recognition in the majority of cases of pre-mRNA splicing in eukaryotes. Mutations at splice junctions often cause exon skipping, short deletions, or insertions in the mature mRNA, underlying one common molecular mechanism of genetic diseases. Using N-ethyl-N-nitrosourea, a novel recessive mutation named seal was produced, associated with fragile bones and susceptibility to fractures (spine and limbs). A single nucleotide transversion (T → A) at the second position of intron 36 of the Col1a1 gene, encoding the type I collagen, α1 chain, was responsible for the phenotype. Col1a1 seal mRNA expression occurred at greatly reduced levels compared to the wild-type transcript, resulting in reduced and aberrant collagen fibers in tibiae of seal homozygous mice. Unexpectedly, splicing of Col1a1 seal mRNA followed the normal pattern despite the presence of the donor splice site mutation, likely due to the action of a putative intronic splicing enhancer present in intron 25, which appeared to function redundantly with the splice donor site of intron 36. Seal mice represent a model of human osteogenesis imperfecta, and reveal a previously unknown mechanism for splicing "rescue."

    DOI: 10.1038/s41598-017-10343-9

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  • Recruitment of bone marrow-derived cells to the periodontal ligament via the stromal cell-derived factor-1/C-X-C chemokine receptor type 4 axis Reviewed

    Kaku, M., Kitami, M., Rosales Rocabado, J.M., Ida, T., Akiba, Y., Uoshima, K.

    Journal of Periodontal Research   52 ( 4 )   686 - 694   2017

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    DOI: 10.1111/jre.12433

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  • Prolonged Survival of Transplanted Osteoblastic Cells Does Not Directly Accelerate the Healing of Calvarial Bone Defects. Reviewed International journal

    Megumi Kitami, Masaru Kaku, Juan Marcelo Rosales Rocabado, Takako Ida, Nami Akiba, Katsumi Uoshima

    Journal of cellular physiology   231 ( 9 )   1974 - 82   2016.9

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    Considering the increased interest in cell-based bone regeneration, it is necessary to reveal the fate of transplanted cells and their substantive roles in bone regeneration. The aim of this study was to analyze the fate of transplanted cells and the effect of osteogenic cell transplantation on calvarial bone defect healing. An anti-apoptotic protein, heat shock protein (HSP) 27, was overexpressed in osteoblasts. Then, the treated osteoblasts were transplanted to calvarial bone defect and their fate was analyzed to evaluate the significance of transplanted cell survival. Transient overexpression of Hsp27 rescued MC3T3-E1 osteoblastic cells from H2 O2 -induced apoptosis without affecting osteoblastic differentiation in culture. Transplantation of Hsp27-overexpressing cells, encapsulated in collagen gel, showed higher proliferative activity, and fewer apoptotic cells in comparison with control cells. After 4-week of transplantation, both control cell- and Hsp27 overexpressed cell-transplanted groups showed significantly higher new bone formation in comparison with cell-free gel-transplantation group. Interestingly, the prolonged survival of transplanted osteoblastic cells by Hsp27 did not provide additional effect on bone healing. The transplanted cells in collagen gel survived for up to 4-week but did not differentiate into bone-forming osteoblasts. In conclusion, cell-containing collagen gel accelerated calvarial bone defect healing in comparison with cell-free collagen gel. However, prolonged survival of transplanted cells by Hsp27 overexpression did not provide additional effect. These results strongly indicate that cell transplantation-based bone regeneration cannot be explained only by the increment of osteogenic cells. Further studies are needed to elucidate the practical roles of transplanted cells that will potentiate successful bone regeneration. J. Cell. Physiol. 231: 1974-1982, 2016. © 2016 Wiley Periodicals, Inc.

    DOI: 10.1002/jcp.25302

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  • Mechanical Loading Stimulates Expression of Collagen Cross-Linking Associated Enzymes in Periodontal Ligament. Reviewed International journal

    Masaru Kaku, Juan Marcelo Rosales Rocabado, Megumi Kitami, Takako Ida, Yosuke Akiba, Mitsuo Yamauchi, Katsumi Uoshima

    Journal of cellular physiology   231 ( 4 )   926 - 33   2016.4

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    Type I collagen, a major extracellular component of the periodontal ligament (PDL), is post-translationally modified by a series of specific enzymes. Among the collagen-modifying enzymes, lysyl oxidase (LOX) is essential to initiate collagen cross-linking and lysyl hydroxylases (LHs) to regulate the cross-linking pathways that are important for tissue specific mechanical properties. The purpose of this study was to investigate the effects of mechanical loading on the expression of collagen-modifying enzymes and subsequent tissue changes in PDL. Primary human PDL cells were subjected to mechanical loading in a 3D collagen gel, and gene expression and collagen component were analyzed. Wistar rats were subjected to excessive occlusal loading with or without intra-peritoneal injection of a LOX inhibitor, β-aminopropionitrile (BAPN). Upon mechanical loading, gene expression of LH2 and LOX was significantly elevated, while that of COL1A2 was not affected on hPDL-derived cells. The mechanical loading also elevated formation of collagen α-chain dimers in 3D culture. The numbers of LH2 and LOX positive cells in PDL were significantly increased in an excessive occlusal loading model. Notably, an increase of LH2-positive cells was observed only at the bone-side of PDL. Intensity of picrosirius red staining was increased by excessive occlusal loading, but significantly diminished by BAPN treatment. These results demonstrated that mechanical loading induced collagen maturation in PDL by up-regulating collagen-modifying enzymes and subsequent collagen cross-linking which are important for PDL tissue maintenance. J. Cell. Physiol. 231: 926-933, 2016. © 2015 Wiley Periodicals, Inc.

    DOI: 10.1002/jcp.25184

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  • Royal jelly affects collagen crosslinking in bone of ovariectomized rats Reviewed

    Kaku, M., Rocabado, J.M.R., Kitami, M., Ida, T., Uoshima, K.

    Journal of Functional Foods   7 ( 1 )   398 - 406   2014

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    DOI: 10.1016/j.jff.2014.01.019

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  • Detection and Partial Characterization of Bone Marrow Derived Cells in Periodontal Ligament Reviewed

    Kaku Masaru, Edris Asadullah Mohammad, Kitami Megumi, Ida Takako, Rocabado J, M. Rosales, Uoshima Katsumi

    JOURNAL OF BONE AND MINERAL RESEARCH   28   2013.2

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MISC

  • 歯根膜発生過程における細胞周期動態のin vivo解析

    井田貴子, 加来賢, 水越優, 北見公平, 魚島勝美

    日本補綴歯科学会誌(Web)   11   250 (WEB ONLY)   2019

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  • 矯正的歯の移動時のマウス歯根膜における増殖/静止期細胞の局在

    水越 優, 加来 賢, 北見 公平, 井田 貴子, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   77回   231 - 231   2018.10

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  • SDF-1/CXCR4による歯根膜への骨髄由来細胞の誘導

    加来 賢, 北見 恩美, Rosales Rocavado J.M., 井田 貴子, 秋葉 陽介, 魚島 勝美

    日本補綴歯科学会誌   8 ( 特別号 )   237 - 237   2016.7

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  • 歯根膜には大腿骨骨髄に由来する幹細胞が存在する

    加来 賢, 北見 恩美, Rosales Rocabado Juan Marcelo, 井田 貴子, 秋葉 陽介, 魚島 勝美

    日本補綴歯科学会誌   6 ( 特別号 )   104 - 104   2014.5

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  • コラーゲン・クロスリンクが骨芽細胞分化、破骨細胞分化に及ぼす影響

    井田 貴子, 加来 賢, 北見 恩美, Rosales Rocabado Juan, Marcelo Rosales, 加来 咲子, 魚島 勝美

    日本補綴歯科学会誌   6 ( 特別号 )   259 - 259   2014.5

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  • 歯根膜における骨髄由来細胞の局在と幹細胞マーカーの発現

    加来 賢, 北見 恩美, 井田 貴子, 秋葉 陽介, 魚島 勝美

    Journal of Oral Biosciences Supplement   2013   134 - 134   2013.9

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    Language:Japanese   Publisher:(一社)歯科基礎医学会  

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  • 移植細胞の初期動態とストレスタンパク質HSP27導入による影響

    北見 恩美, 加来 賢, 井田 貴子, 秋葉 陽介, 魚島 勝美

    Journal of Oral Biosciences Supplement   2013   155 - 155   2013.9

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  • 移植細胞の初期動態とHSP27過剰発現骨芽細胞に関する分析

    北見 恩美, 加来 賢, 井田 貴子, 秋葉 陽介, 魚島 勝美

    新潟歯学会雑誌   43 ( 1 )   73 - 74   2013.6

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  • 歯根膜における骨髄由来細胞の局在と幹細胞マーカーの発現

    加来 賢, 野澤 恩美, Rosales Juan Marcelo, 井田 貴子, 秋葉 陽介, 魚島 勝美

    日本補綴歯科学会誌   5 ( 特別号 )   222 - 222   2013.5

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  • 移植細胞の初期動態とHSP27の導入による細胞移植法の検討

    野澤 恩美, 加来 賢, Rosales Juan Marcelo, 井田 貴子, 秋葉 陽介, 魚島 勝美

    日本補綴歯科学会誌   5 ( 特別号 )   248 - 248   2013.5

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  • 歯根膜の部位によるプライマリー・シリア出現率の違いと過剰咬合による変化

    井田 貴子, 加来 賢, 野澤 恩美, Rosales Juan Marcelo, 加来 咲子, 魚島 勝美

    日本補綴歯科学会誌   5 ( 特別号 )   209 - 209   2013.5

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  • Early Behaviors of Transplanted Cells and the Effect of HSP27 on the Cell Survival

    Megumi Kitami, Masaru Kaku, Takako Ida, J. M. Rosales, Kastumi Uoshima

    JOURNAL OF BONE AND MINERAL RESEARCH   28   2013.2

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:WILEY-BLACKWELL  

    Web of Science

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  • 移植細胞の初期動態とストレスタンパク質HSP27導入による影響

    北見恩美, 北見恩美, 加来賢, 井田貴子, 秋葉陽介, 秋葉陽介, 魚島勝美, 魚島勝美

    Journal of Oral Biosciences Supplement (Web)   2013   2013

  • 歯根膜における骨髄由来細胞の局在と幹細胞マーカーの発現

    加来賢, 北見恩美, 井田貴子, 秋葉陽介, 秋葉陽介, 魚島勝美, 魚島勝美

    Journal of Oral Biosciences Supplement (Web)   2013   2013

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Awards

  • 奨励論文賞

    2019   公益社団法人日本補綴歯科学会  

    Ida T, Kaku M, Kitami M, Terajima M, Rosales Rocabado JM, Akiba Y, Nagasawa M, Yamauchi M, Uoshima K

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  • Best Oral Presentation Award

    2014.7   The Asian Academy of Osseointegration  

    Takako Ida, Masaru Kaku, Megumi Kitami, JM Rosales Rocabado, Katsumi Uoshima

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Research Projects

  • 歯根膜線維の組織化が細胞外微小環境として幹細胞分化に及ぼす影響の解明

    Grant number:20K18595  2020.4 - 2022.3

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    井田 貴子

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    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

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  • Development of a novel cellulose scaffold to potentiate the transplanted cells survival for bone regeneration

    Grant number:18K09680  2018.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

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    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

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  • Elucidation of fate path and regulatory factors of periodontal ligament tissue stem cells

    Grant number:18H02989  2018.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

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    Grant amount:\17420000 ( Direct Cost: \13400000 、 Indirect Cost:\4020000 )

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  • Functional elucidation of SPARC-DDR2 pathway on collagen fiber maturation of periodontal ligament which is induced by mechanical stress

    Grant number:18K17142  2018.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists  Grant-in-Aid for Early-Career Scientists

    Ida Takako

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    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    The effect of DDR2 inhibitor on collagen fiber formation has been studied using mouse periodontal ligament-derived cells. The periodontal ligament-derived cells were cultured in the presence of DDR2 inhibitor (AZ628), LOX gene expression and LOX protein enzyme activity were measured, and the maturity of the matrix was evaluated by Picrosirius red staining.
    Since SPARC has a calcium binding site in its domain, the low calcium rat models were used. As a result of bone structure analysis using 3D images obtained by μCT, bone density was decreased in the cortical bone and cancellous bone of the maxilla in the low calcium rat group compared with the normal diet group.

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  • 力学的刺激に誘導される歯根膜線維の成熟におけるSPARC-DDR2経路の機能解明

    2018.4 - 2020.3

    独立行政法人日本学術振興会 

    井田 貴子

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    Authorship:Principal investigator  Grant type:Competitive

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  • コラーゲンの2次構造が骨代謝に及ぼす影響とそのメカニズム探索

    Grant number:15J03831  2015.4 - 2017.3

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    井田 貴子

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    Grant amount:\1700000 ( Direct Cost: \1700000 )

    昨年度申請者は細胞培養系においてコラーゲン・クロスリンクの変化が骨芽細胞、破骨細胞、骨髄由来間質細胞に及ぼす影響について解析し、クロスリンクの低下したマトリックス上に播種した各細胞の増殖能・分化能は上昇することを明らかにした。動物実験に関しては、4週齢のC57BL/6JマウスをBAPN含有飼料またはコントロール飼料にて8週間飼育して得たクロスリンク形成不全マウスに関して、通常飼料に戻して4週後の大腿骨において、BAPN摂取群における骨芽細胞の活性は有意に上昇したが、破骨細胞の活性に変化は認められなかったことを報告した。本年度はクロスリンク形成不全マウスより全血を採取して血清骨代謝マーカーの解析を行った。BAPN摂取群において、骨形成マーカーであるP1NPは8週に、Osteocalcinは4週において増加が認められた。そこで、マイクロCTを用いた骨形態計測解析において増加が見られた骨組織体積および血清中骨形成マーカーに関して、Pearsonの相関係数について解析を行ったところ、4週のBAPN高摂取群において、両変数間に高い正の相関がみられ、クロスリンクの変化により骨芽細胞の挙動が影響を受ける可能性が示唆された。
    本研究結果よりコラーゲン・クロスリンクは骨の機械的特性を維持する構造としてのみならず、骨芽細胞、破骨細胞、骨髄由来間質細胞の分化に影響を及ぼすことにより、局所的な骨代謝に影響を及ぼす可能性が示唆された。これらを制御するメカニズムとしては、細胞外マトリックスの弾性率が細胞の分化制御に関わるとの報告もあることから、クロスリンクの低下による基質の弾性率変化が細胞挙動に影響を及ぼしている可能性も考えられるが、引き続き受容体などの分子メカニズムに関する解析が必要である。

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  • コラーゲンの2次構造が骨代謝に及ぼす影響とそのメカニズム探索

    2015.4 - 2017.3

    独立行政法人日本学術振興会 

    井田 貴子

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    Authorship:Principal investigator  Grant type:Competitive

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Teaching Experience

  • 保存修復学実習

    2022
    Institution name:新潟大学

  • 早期臨床実習II

    2022
    Institution name:新潟大学

  • 生体材料学

    2021
    Institution name:新潟大学