Updated on 2022/12/01

写真a

 
SHIMAMOTO Saki
 
Organization
Academic Assembly Institute of Science and Technology NOUGAKU KEIRETSU Assistant Professor
Faculty of Agriculture Department of Agriculture Assistant Professor
Title
Assistant Professor
Contact information
メールアドレス
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Degree

  • 農学 ( 2019.9   鹿児島大学 )

Research Interests

  • 畜産物利用

  • 骨格筋タンパク質分解

  • 飼料化学

  • 栄養生化学

Research Areas

  • Life Science / Applied biochemistry

  • Life Science / Animal production science

  • Life Science / Animal production science

Research History (researchmap)

  • 日本学術振興会 特別研究員PD

    2019.10 - 2020.2

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  • 日本学術振興会 特別研究員DC1

    2017.4 - 2019.9

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Research History

  • Niigata University   Faculty of Agriculture Department of Agriculture   Assistant Professor

    2020.3

Education

  • Kagoshima University   United Graduate School of Agricultural Sciences   応用生命科学専攻

    - 2019.9

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  • Kagoshima University   Graduate School of Agriculture   生物資源化学専攻

    - 2017.3

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  • Kagoshima University   Faculty of Agriculture   Biochemical Science and Technology

    - 2015.3

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Professional Memberships

Committee Memberships

  • 日本畜産学会   若手企画委員  

    2021.4   

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  • 全日本大学対抗ミートジャッジング競技会   実行委員会委員  

    2018.10   

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    Committee type:Other

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Papers

  • Effects of a Dried Neem Leaf Extract on the Growth Performance, Meat Yield and Meat Quality in Skeletal Muscle of Broiler Chickens Under High-Temperature Conditions

    Kiriko Nakamura, Ayumi Katafuchi, Saki Shimamoto, Goichiro Ogawa, Nikhil Khandelwal, Kenji Tatsugawa, Yoshikazu Fujita, Akira Ohtsuka, Daichi Ijiri

    Frontiers in Animal Science   3   2022.6

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    Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media SA  

    We aimed to examine the effects of cyclical high ambient temperature (HT) and dried Neem (Azadirachta indica) leaf extract (DNE) supplementation on the growth performance, muscle lipid peroxidation level, and muscle drip loss of broiler chickens. Twenty-four 15-day old broiler chickens (Chunky strain ROSS 308) were divided into four treatment groups that were fed diets with or without 2.0% DNE under thermoneutral (25 ± 1°C) or cyclical HT (35 ± 1°C for 8 h/day) conditions. Supplementation of DNE did not affect the growth performance of the chicks, but HT reduced their feed intake, the weights of breast muscle and heart. In addition, supplementation with DNE ameliorated the negative effects of cyclical HT on feed intake and breast muscle mass. Furthermore, cyclical HT increased the malondialdehyde (MDA) concentration and drip loss over 48 h of storage of the breast muscle, and these effects were ameliorated by DNE. Collectively, we conclude that dietary supplementation with DNE reduces the muscle MDA concentration and drip loss of broiler chickens kept under HT conditions.

    DOI: 10.3389/fanim.2022.914772

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  • Suppression of FoxO1 mRNA by β2 -adrenoceptor-cAMP signaling through miR-374b-5p and miR-7a-1-3p in C2C12 myotubes. Reviewed International journal

    Saki Shimamoto, Kazuki Nakashima, Nao Nishikoba, Rukana Kohrogi, Akira Ohtsuka, Shinobu Fujimura, Daichi Ijiri

    FEBS open bio   12 ( 3 )   627 - 637   2022.3

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    β2 -Adrenoceptor (β2 -AR) signaling decreases the transcriptional activity of forkhead box O (FoxO), but the underlying mechanisms remain incompletely understood. Here, we investigated how β2 -AR signaling regulates the protein abundance of FoxO and its transcriptional activity in skeletal muscle. We observed that stimulation of β2 -AR with its selective agonist, clenbuterol, rapidly decreased FoxO1 mRNA expression, and this was accompanied by a decrease in either FoxO1 protein level or FoxO transcriptional activity. We subsequently observed that miR-374b-5p and miR-7a-1-3p were rapidly upregulated in response to β2 -AR stimulation. Transfection with mimics of either miRNA successfully decreased FoxO1 mRNA. Moreover, because β2 -AR stimulation increased cAMP concentration, pretreatment with an adenylyl cyclase inhibitor canceled out these effects of β2 -AR stimulation. These results suggest that β2 -AR stimulation results in rapid upregulation of miR-374b-5p and miR-7a-1-3p in myotubes, which in turn results in a decrease in FoxO1 mRNA expression via the β2 -AR-cAMP signaling pathway.

    DOI: 10.1002/2211-5463.13368

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  • Impact on genetic differences among various chicken breeds on free amino acid contents of egg yolk and albumen. Reviewed International journal

    Tatsuhiko Goto, Saki Shimamoto, Masahiro Takaya, Shun Sato, Kanna Takahashi, Kenji Nishimura, Yasuko Morii, Kyoko Kunishige, Akira Ohtsuka, Daichi Ijiri

    Scientific reports   11 ( 1 )   2270 - 2270   2021.1

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    Eggs play important roles as food resources and nutraceuticals, to alleviate malnutrition and to improve health status in the world. Since free amino acids contribute to the nutritional values and food tastes, we investigated a total of 81 eggs from five chicken breeds, which are Australorp, Nagoya (NGY), Rhode Island Red (RIR), Shamo (SHA), Ukokkei, and two F1 hybrids (NGYxRIR and SHAxRIR) to test impact on genetic differences in 10 egg traits, 20 yolk amino acid traits, and 18 albumen amino acid traits. One-way ANOVA revealed significant breed effects on 10 egg traits, 20 yolk amino acid traits, and 15 albumen amino acid traits. Moreover, a significant heterosis effect on yolk aspartic acid was identified. In addition, positive correlations were found broadly among traits within each trait category (egg traits, yolk amino acid traits, and albumen amino acid traits), whereas there were basically no or weak correlations among the trait categories. These results suggest that almost all traits can be dramatically modified by genetic factor, and there will be partially independent production systems of amino acids into yolk and albumen. Since there will be typical quantitative genetic architecture of egg contents, further genetic analyses will be needed.

    DOI: 10.1038/s41598-021-81660-3

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  • Analyses of free amino acid and taste sensor traits in egg albumen and yolk revealed potential of value-added eggs in chickens. Reviewed International journal

    Tatsuhiko Goto, Saki Shimamoto, Akira Ohtsuka, Daichi Ijiri

    Animal science journal = Nihon chikusan Gakkaiho   92 ( 1 )   e13510   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    To create high-quality eggs by using different breed and feed materials, we investigated free amino acid contents and taste sensor traits using two chicken breeds (Rhode Island Red; RIR and Australorp; AUS) fed two feeds (mixed and fermented feeds). Two-way ANOVA revealed significant breed and feed main and interaction effects on albumen bitterness and a significant interaction effect on yolk bitterness. Albumen from RIR fed mixed feed and AUS fed fermented feed was higher bitterness, whereas yolk from those groups was lower bitterness. Significant breed effects were detected in four albumen amino acid traits (His, Met, Ile, and Lys) and a yolk His, whereas significant feed effects were found in 15 albumen amino acid traits (Asp, Glu, Ser, His, Gly, Thr, Ala, Tyr, Val, Met, Trp, Ile, Leu, Lys, and Pro) and a yolk cystine trait. Compared to albumen amino acids, yolk amino acids had limited effects by breed and feed. The present results suggest that genetic and nutritional factors can alter not only amino acid contents but also sensor values of bitterness, indicating that selecting the combination of breed and feed enable us to make amino acids enriched and taste added designer eggs in future.

    DOI: 10.1111/asj.13510

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  • Genetic effect on free amino acid contents of egg yolk and albumen using five different chicken genotypes under floor rearing system. Reviewed International journal

    Kenji Nishimura, Daichi Ijiri, Saki Shimamoto, Masahiro Takaya, Akira Ohtsuka, Tatsuhiko Goto

    PloS one   16 ( 10 )   e0258506   2021

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    Language:English   Publishing type:Research paper (scientific journal)  

    Chicken eggs play an important role as food resources in the world. Although genetic effects on yolk and albumen contents have been reported, the number of chicken genotypes analyzed so far is still limited. To investigate the effect of genetic background on 10 egg traits, 19 yolk amino acid traits, and 19 albumen amino acid traits, we evaluated a total of 58 eggs from five genotypes: two Japanese indigenous breeds (Ukokkei and Nagoya) and three hybrids (Araucana cross, Kurohisui, and Boris Brown) under a floor rearing system. One-way ANOVA revealed significant effects of genotype on 10 egg traits, 8 yolk amino acids (Asp, Glu, Ser, Gly, Thr, Tyr, Cys, and Leu), and 11 albumen amino acids (Asp, Glu, Asn, Ser, Gln, His, Ala, Tyr, Trp, Phe, and Ile) contents. Moderate to strong positive phenotypic correlations among traits within each trait category (size and weight traits, yolk amino acid traits, and albumen amino acid traits), whereas there were basically no or weak correlations among the trait categories. However, a unique feature was found in the Araucana cross indicating moderate positive correlations of amino acids between yolk and albumen. These results suggest that genetic factors can modify not only the size and weight of the egg and eggshell color but also yolk and albumen free amino acids contents.

    DOI: 10.1371/journal.pone.0258506

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  • Effects of Supplementation with Dried Neem Leaf Extract on Lipid Peroxidation and Antioxidant Enzyme mRNA Expression in the Pectoralis Major Muscle of Broiler Chickens Reviewed

    Kiriko Nakamura, Mitsuki Shishido, Saki Shimamoto, Goichiro Ogawa, Nikhil Khandelwal, Kenji Tatsugawa, Yoshikazu Fujita, Akira Ohtsuka, Daichi Ijiri

    The Journal of Poultry Science   59 ( 1 )   75 - 80   2021

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Japan Poultry Science Association  

    This study was conducted to evaluate the effects of dietary supplementation of dried neem (Azadirachta indica) leaf extract (DNE) on lipid peroxidation and the expression of genes encoding mRNAs in antioxidant enzymes in the pectoralis major muscle of chickens. A total of 24 male broiler chickens (ROSS308) were divided into three groups (n=8) at 21 days of age. The control group of chickens was fed a basal diet, and the remaining two groups of chickens were fed a basal diet supplemented with DNE at a concentration of 0.5% or 2.0% until 35 days of age. Growth performance (body weight, weight gain, feed intake, and feed conversion ratio) and tissue weights did not differ among the three groups. The 2.0% DNE-supplemented diet decreased the muscle malondialdehyde content, a marker of lipid peroxidation, and drip loss compared to the control chickens. In addition, the expression of genes encoding mRNAs of antioxidant enzymes (i.e., Cu/Zn-superoxide dismutase, Mn-superoxide dismutase, glutathione peroxidase 7, and catalase) were higher in the pectoralis major muscle of chickens fed the 2.0% DNE-supplemented diet than in the control chickens. Therefore, DNE supplementation increased the expression of genes encoding mRNAs in antioxidant enzymes and reduced lipid peroxidation and drip loss in the pectoralis major muscle of broiler chickens.

    DOI: 10.2141/jpsa.0200120

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  • Insulin Stimulation of Protein Synthesis and mTOR Signaling in Chick Myotube Cultures. Reviewed

    Kazuki Nakashima, Aiko Ishida, Saki Shimamoto, Daichi Ijiri, Akira Ohtsuka

    The journal of poultry science   57 ( 3 )   205 - 209   2020.7

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    Insulin stimulates protein synthesis in skeletal muscles. Protein synthesis is controlled by the mechanistic target of rapamycin (mTOR) signaling in skeletal muscles. This study was conducted to investigate the effect of insulin on protein synthesis and mTOR signaling in chick myotube cultures. Chick myotubes were incubated with insulin (1 µg/ml) for 1 h. Protein synthesis, measured using the surface sensing of translation method, was significantly increased by insulin. The phosphorylation of AKT (Thr308 and Ser473), p70 ribosomal S6 kinase 1 (S6K1, Thr389), S6 ribosomal protein (Ser235/236), and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1, Thr37/46) was also significantly increased by insulin. These results suggest that insulin stimulates protein synthesis via mTOR signaling (phosphorylation of AKT, S6K1, S6 ribosomal protein, and 4E-BP1) in chick myotube cultures.

    DOI: 10.2141/jpsa.0190082

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  • Effects of Cyclic High Ambient Temperature and Dietary Supplementation of Orotic Acid, a Pyrimidine Precursor, on Plasma and Muscle Metabolites in Broiler Chickens. Reviewed International journal

    Saki Shimamoto, Kiriko Nakamura, Shozo Tomonaga, Satoru Furukawa, Akira Ohtsuka, Daichi Ijiri

    Metabolites   10 ( 5 )   2020.5

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    The aim of this study was to evaluate the effects of high ambient temperature (HT) and orotic acid supplementation on the plasma and muscle metabolomic profiles in broiler chickens. Thirty-two 14-day-old broiler chickens were divided into four treatment groups that were fed diets with or without 0.7% orotic acid under thermoneutral (25 ± 1 °C) or cyclic HT (35 ± 1 °C for 8 h/day) conditions for 2 weeks. The chickens exposed to HT had higher plasma malondialdehyde concentrations, suggesting an increase in lipid peroxidation, which is alleviated by orotic acid supplementation. The HT environment also affected the serine, glutamine, and tyrosine plasma concentrations, while orotic acid supplementation affected the aspartic acid, glutamic acid, and tyrosine plasma concentrations. Untargeted gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS)-based metabolomics analysis identified that the HT affected the plasma levels of metabolites involved in purine metabolism, ammonia recycling, pyrimidine metabolism, homocysteine degradation, glutamate metabolism, urea cycle, β-alanine metabolism, glycine and serine metabolism, and aspartate metabolism, while orotic acid supplementation affected metabolites involved in pyrimidine metabolism, β-alanine metabolism, the malate-aspartate shuttle, and aspartate metabolism. Our results suggest that cyclic HT affects various metabolic processes in broiler chickens, and that orotic acid supplementation ameliorates HT-induced increases in lipid peroxidation.

    DOI: 10.3390/metabo10050189

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  • Feeding the outer bran fraction of rice alters hepatic carbohydrate metabolism in rats. Reviewed International journal

    Kawaguchi M, Nishikoba N, Shimamoto S, Tomonaga S, Kohrogi R, Yamauchi Y, Fujita Y, Ohtsuka A, Ijiri D

    Nutrients   12 ( 2 )   430   2020.2

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    Dietary intake of fiber-rich food has been reported to contribute to multiple health benefits. The aim of the current study is to investigate the effects of a diet containing the outer bran fraction of rice (OBFR), which is rich in insoluble fiber, on the intestinal environment and metabolite profiles of rats. Fourteen 8-week-old male Sprague-Dawley rats were divided into a control group and an OBFR group. For a period of 21 days, the control group was fed a control diet, while the OBFR group was fed a diet containing 5% OBFR. Metabolomics analysis revealed drastic changes in the cecal metabolites of the rats fed the OBFR diet. Furthermore, in the plasma and liver tissue, the concentrations of metabolites involved in pyruvate metabolism, the pentose phosphate pathway, gluconeogenesis, or valine, leucine, isoleucine degradation were changed. Concordantly, the OBFR diet increased the expression of genes encoding enzymes involved in these metabolic pathways in the livers of the rats. Collectively, these results suggest that the OBFR diet altered the concentrations of metabolites in the cecal contents, plasma, and liver, and the hepatic gene expressions of rats, and that this may have mainly contributed to carbohydrate metabolism in the liver.

    DOI: 10.3390/nu12020430

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  • Effects of delaying post-hatch feeding on lipid peroxidation and antioxidant enzyme mRNA expression in the pectoralis major muscle of newly hatched chicks. Reviewed

    Rukana Kohrogi, Saki Shimamoto, Kazuki Nakashima, Daichi Sonoda, Akira Ohtsuka, Daichi Ijiri

    Animal Science Journal   91   e13327   2020.1

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  • Insulin acutely increases glucose transporter 1 on plasma membranes and glucose uptake in an AKT-dependent manner in chicken adipocytes. Reviewed International journal

    Shimamoto S, Nakashima K, Kamimura R, Kohrogi R, Inoue H, Nishikoba N, Ohtsuka A, Ijiri D

    General and Comparative Endocrinology   283   113232 - 113232   2019.11

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    Avian glucose transporters (GLUT) responsible for insulin-responsive glucose uptake into adipocytes remain poorly characterized. We aimed to identify the insulin-responsive GLUT using primary culture of chicken adipocytes. Acute stimulation with 1 μM insulin for 20 min increased 2-deoxyglucose uptake, AKT protein phosphorylation, and GLUT1 protein levels on the plasma membrane of the chicken adipocytes, whereas pretreatment with 10 μM triciribine, an AKT inhibitor, canceled these effects. Furthermore, the insulin stimulation did not affect GLUT12 protein levels on the plasma membrane of the chicken adipocytes. Our results suggest that GLUT1 is an insulin-responsive GLUT in chicken adipocytes.

    DOI: 10.1016/j.ygcen.2019.113232

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  • Effects of Delayed Feeding on the Performance of Laying Hens Rraised under Thermoneutral and High Ambient Temperature Conditions Reviewed

    Rukana Kohrogi, Nao Nishikoba, Saki Shimamoto, Ryo Kamimura, Kazuki Nakashima, Osamu Tada, Akira Ohtsuka, Daichi Ijiri

    Japanese Poultry Science   56 ( J1 )   J1‐J6   2019.4

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  • Effects of astaxanthin-rich dried cell powder from Paracoccus carotinifaciens on carotenoid composition and lipid peroxidation in skeletal muscle of broiler chickens under thermo-neutral or realistic high temperature conditions. Reviewed International journal

    Inoue H, Shimamoto S, Takahashi H, Kawashima Y, Wataru S, Ijiri D, Ohtsuka A

    Animal science journal = Nihon chikusan Gakkaiho   90 ( 2 )   229 - 236   2019.2

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    Thirty-two 15-day old broiler chicks (Chunky strain ROSS 308) were randomly divided into four treatments in a 2 × 2 factorial design. The main factors were diet (basal diet or basal diet supplemented with 0.15% astaxanthin-rich dried cell powder (Panaferd-P [astaxanthin 30 ppm]) and ambient temperature (thermo-neutral [25 ± 1°C] or high [35 ± 1°C for 6 hr]). Dietary supplementation with Panaferd-P did not affect growth performance, though high ambient temperature decreased feed intake and the weight of breast tender muscle, liver, and heart. High ambient temperature also decreased redness in both breast and leg muscles of chickens, while Panaferd-P increased redness and yellowness of breast and leg muscles of chickens. Panaferd-P increased Paracoccus carotinifaciens-derived pigments (i.e., adonixanthin, astaxanthin, adonirubin, and cantaxanthin) as well as corn-derived pigments such as zeaxanthin and lutein in breast and leg muscles. High ambient temperature increased the malondialdehyde (MDA) concentration in breast muscle, while Panaferd-P decreased the MDA concentration in breast muscle under both temperature conditions. Our results suggest that dietary supplementation with Panaferd-P increases muscle carotenoid content, the redness and yellowness of meat and decreases the muscle MDA concentration in broiler chickens kept under thermo-neutral or high ambient temperature conditions.

    DOI: 10.1111/asj.13141

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  • Effects of delayed feeding on lipid peroxidation, drip losses, color, and taste of chicken breast meat Reviewed

    Daichi Ijiri, Naoki Hozo, Saki Shimamoto, Mana Kawaguchi, Airi Furukawa, Osamu Tada, Shozo Tomonaga, Kazuki Nakashima, Akira Ohtsuka

    Nihon chikusan Gakkaiho   89 ( 2 )   191‐198(J‐STAGE)   2018

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  • The β2-adrenergic receptor is involved in differences in the protein degradation level of the pectoral muscle between fast- and slow-growing chicks during the neonatal period Reviewed

    Saki Shimamoto, Daichi Ijiri, Kazuki Nakashima, Mana Kawaguchi, Akira Ohtsuka

    General and Comparative Endocrinology   267   45 - 50   2018

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Academic Press Inc.  

    The aim of this study was to investigate whether β2-AR mRNA expression is involved in either atrogin-1/MAFbx mRNA expression or protein degradation in chicken skeletal muscle by comparing fast- and slow-growing chicks during the neonatal period. Based on their body weight gain from 1 to 5 days of age, 5-day-old chicks (Gallus gallus domestics) were divided into a slow-growing and a fast-growing group, the mean weight gains of which were 6.3 ± 1.3 g/day and 11.3 ± 0.9 g/day, respectively. The ratio of pectoral muscle weight to total body weight was higher in the fast-growing group of chicks than in the slow-growing group. In addition, the plasma 3-methylhistidine concentration, an index of protein degradation in skeletal muscle, was significantly lower in the fast-growing than in the slow-growing chicks. The mRNA expression of β2-AR, which we previously found is involved in decreasing muscle protein degradation by suppression atrogin-1/MAFbx mRNA expression, was significantly higher in the pectoral muscle of the fast-growing group compared with that of the slow-growing group. Concordantly, lower mRNA expression of atrogin-1/MAFbx was observed in the pectoral muscle of the fast-growing chicks. However, in the sartorius muscle, which is a muscle in the thigh, the ratio of the muscle weight to total body weight was not significantly different between the two groups of chicks at 5 days of age. In addition, there was no significant difference in the mRNA expressions of β2-AR and atrogin-1/MAFbx in the sartorius muscle between these two groups. These results suggest that β2-AR expression levels might be physiologically significant in the control of protein degradation in the pectoral muscle of neonatal chicks.

    DOI: 10.1016/j.ygcen.2018.05.028

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  • beta(1)- and beta(2)-adrenergic receptor stimulation differ in their effects on PGC-1 alpha and atrogin-1/MAFbx gene expression in chick skeletal muscle Reviewed

    Saki Shimamoto, Daichi Ijiri, Mana Kawaguchi, Kazuki Nakashima, Osamu Tada, Hiroki Inoue, Akira Ohtsuka

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY   211   1 - 6   2017.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE INC  

    Adrenaline changes expression of the genes encoding peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha), which is known as a regulator of muscle size, and atrogin-1/muscle atrophy F-box (MAFbx), which is a muscle-specific ubiquitin ligase. However, the subtype of beta-adrenergic receptor (beta-AR) involved in regulating these genes in skeletal muscle is not yet well defined. In this study, the effects of intraperitoneal injection of adrenaline and three beta(1-3)-AR selective agonists on chick skeletal muscle metabolism were examined, to evaluate the functions of beta-AR subtypes. Adrenaline decreased atrogin-1/MAFbx mRNA levels accompanied by an increase in PGC-1 alpha mRNA and protein levels. However, among the three selective agonists, only the beta(1)-AR agonist, dobutamine, increased PGC-1 alpha mRNA and protein levels, while the beta(2)-AR agonist, clenbuterol, suppressed atrogin-1/MAFbx mRNA levels. In addition, preinjection of the beta(1)-AR antagonist, acebutolol, and the beta(2)-AR antagonist, butoxamine, inhibited the adrenaline-induced increase in PGC-1 alpha mRNA levels and the decrease in atrogin-1/MAFbx mRNA levels, respectively. Compared with adrenaline administration, the beta(3)-AR agonist, BRL37344, decreased PGC-1 alpha mRNA levels and increased atrogin-1/MAFbx mRNA levels. These results suggest that, in chick skeletal muscle, PGC-1 alpha is induced via the 1.35-AR, while atrogin-1/MAFbx is suppressed via the beta(2)-AR.

    DOI: 10.1016/j.cbpa.2017.05.013

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  • Effects of Insulin-Like Growth Factor-I on the Expression of Atrogin-1/MAFbx in Chick Myotube Cultures Reviewed

    Kazuki Nakashima, Aiko Ishida, Saki Shimamoto, Daichi Ijiri, Akira Ohtsuka

    JOURNAL OF POULTRY SCIENCE   54 ( 3 )   247 - 252   2017.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPAN POULTRY SCIENCE ASSOC  

    The expression of atrogin-1/MAFbx, a muscle-specific E3 ubiquitin ligase, is increased in catabolic conditions that result in muscle atrophy. The expression of atrogin-1/MAFbx mRNA is also decreased by the insulin-like growth factor-I (IGF-I) in mammalian skeletal muscle cell cultures. This study investigated the effect of IGF-I on the expression of atrogin-1/MAFbx in chicken skeletal muscle cell cultures. Chick myotubes were incubated with IGF-I for 1, 6, or 24 h. Protein content was increased by IGF-I (100 ng/ml) and incubated for 24 h in chick myotubes. The expression of atrogin-1/MAFbx mRNA decreased in the presence of IGF-I (1, 10, and 100 ng/ml) for 6h in chick myotubes. The expression of the m-calpain large subunit and cathepsin B mRNA was not decreased by IGF-I. Phosphorylation of Akt and FOXO1 increased in the presence of IGF-I (100 ng/ml) for 1 h in chick myotubes. These results indicate that IGF-I suppresses atrogin-1/MAFbx mRNA expression by phosphorylation of Akt and FOXO1, resulting in an increase in muscle growth in chick myotube cultures.

    DOI: 10.2141/jpsa.0160141

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  • Effects of first exogenous nutrients on the mRNA levels of atrogin-1/MAFbx and GLUT1 in the skeletal muscles of newly hatched chicks Reviewed

    Daichi Ijiri, Said Shimamoto, Mana Kawaguchi, Airi Furukawa, Kazuki Nakashima, Osamu Tada, Akira Ohtsuka

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY   205   8 - 14   2017.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE INC  

    The aim of this study was to examine the effects of first exogenous nutrients on the mRNA levels of muscle atrophy F-box (atrogin-1/MAFbx) and glucose transporters (GLUTs) in the skeletal muscles of newly hatched chicks with no feed experience. In experiment 1, newly hatched chicks had free access to feed or were fasted for the first 24 h. The chicks having free access to feed for the first 24 h increased their body weight and had decreased atrogin-1/MAFbx mRNA levels in their sartorius and pectoralis major muscles compared with the fasted chicks. In experiment 2, newly hatched chicks received a single feed via intubation into the crop. Three hours after intubation, levels of atrogin-1/MAFbx mRNA in the sartorius muscle were decreased whereas the plasma insulin concentration and phosphorylated Ala levels in the sartorius muscle were increased. In addition, the mRNA levels of GLUT1 and GLUT8 were increased in the-sartorius muscle after the intubation. However, in the pectoralis major muscle, ART phosphorylation and levels of atrogin-1/MAFbx, GLUT1 and GLUT8 mRNA were not affected 3 h after intubation. The first exogenous nutrients increased the level of phosphorylated ART in the sartorius muscle of newly hatched chicks, possibly because of the decrease in atrogin-1/MAFbx mRNA levels. Furthermore, the sartorius muscle in newly hatched chicks appeared to be more susceptible to the first feed compared with the pectoralis major muscle.(C) 2016 Published by Elsevier Inc.

    DOI: 10.1016/j.cbpa.2016.12.014

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  • Single injection of clenbuterol into newly hatched chicks decreases abdominal fat pad weight in growing broiler chickens Reviewed

    Daichi Ijiri, Kanae Ishitani, Mahmoud Mohamed Hamza El-Deep, Mana Kawaguchi, Saki Shimamoto, Yoshitaka Ishimaru, Akira Ohtsuka

    ANIMAL SCIENCE JOURNAL   87 ( 10 )   1298 - 1303   2016.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    The aim of the current study was to examine the effects of clenbuterol injection into newly hatched chicks on both the abdominal fat pad tissue weight and the skeletal muscle weight during subsequent growth. Twenty-seven 1-day-old chicks were divided into two groups, receiving either a single intraperitoneal (i.p.) injection of clenbuterol (0.1mg/kg body weight) or phosphate-buffered saline (PBS). Body weight gain, feed intake and feed conversion ratio were not affected by clenbuterol injection during the 5-week experimental period, while the abdominal fat pad tissue weight of the clenbuterol-injected chicks was lower than that of the control chicks at 5 weeks post-injection. Plasma non-esterified fatty acid concentrations were significantly increased in the clenbuterol-injected chicks, while plasma triacylglycerol concentrations did not differ. Additionally, the enzymatic activity of fatty acid synthase was lower in the liver of the clenbuterol-injected chicks. Conversely, the skeletal muscle weights were not affected by clenbuterol injection. These results suggest that a single clenbuterol injection into 1-day-old chicks decreases the abdominal fat pad tissue weight, but may not affect skeletal muscle weights during growth.

    DOI: 10.1111/asj.12541

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  • Clenbuterol changes phosphorylated FOXO1 localization and decreases protein degradation in the sartorius muscle of neonatal chicks Reviewed

    Saki Shimamoto, Daichi Ijiri, Kazuki Nakashima, Mana Kawaguchi, Yoshitaka Ishimaru, Airi Furukawa, Akira Ohtsuka

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   80 ( 8 )   1499 - 1504   2016.8

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    To investigate the intracellular signaling mechanisms by which clenbuterol reduces muscle protein degradation, we examined the phosphorylation level and intracellular localization of FOXO1 in the sartorius muscle of neonatal chicks. One-day-old chicks were given a single intraperitoneal injection of clenbuterol (0.1mg/kg body weight). Three hours after injection, AKT protein was phosphorylated in the sartorius muscle by clenbuterol injection. Coincidentally, clenbuterol increased cytosolic level of phosphorylated FOXO1 protein, while it decreased nuclear level of FOXO1 protein in the sartorius muscle. Furthermore, clenbuterol decreased the expression of mRNAs for muscle-specific ubiquitin ligases (atrogin-1/MAFbx and MuRF1) in the sartorius muscle accompanied by decreased plasma 3-methylhistidine concentration, an index of muscle protein degradation, at 3h after injection. These results suggested that, in the sartorius muscle of the chicks, clenbuterol changed the intracellular localization of phosphorylated FOXO1, and consequently decreased protein degradation via suppressing the expression of genes encoding muscle-specific ubiquitin ligases.

    DOI: 10.1080/09168451.2016.1158629

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  • Gene expression pattern of glucose transporters in the skeletal muscles of newly hatched chicks Reviewed

    Saki Shimamoto, Daichi Ijiri, Mana Kawaguchi, Kazuki Nakashima, Akira Ohtsuka

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   80 ( 7 )   1382 - 1385   2016.7

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    The gene expression pattern of the glucose transporters (GLUT1, GLUT3, GLUT8, and GLUT12) among pectoralis major and minor, biceps femoris, and sartorius muscles from newly hatched chicks was examined. GLUT1 mRNA level was higher in pectoralis major muscle than in the other muscles. Phosphorylated AKT level was also high in the same muscle, suggesting a relationship between AKT and GLUT1 expression.

    DOI: 10.1080/09168451.2016.1162088

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  • The effects of acute cold exposure on morphology and gene expression in the heart of neonatal chicks Reviewed

    Tomoko Matsubara, Saki Shimamoto, Daichi Ijiri, Akira Ohtsuka, Yukio Kanai, Miho Hirabayashi

    JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY   186 ( 3 )   363 - 372   2016.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER HEIDELBERG  

    Cold exposure induces an increase in blood flow and blood pressure, and long-term exposure to cold causes cardiac hypertrophy. Neonatal chicks (Gallus gallus domesticus) are highly sensitive to cold exposure, because their capacity for thermogenesis is immature until 1 week after hatching. Hence, we hypothesized that the heart of chicks at around 1 week of age acutely responds to cold environment. To investigate the effect of acute (24 h) and long-term (2 weeks) cold on the heart of chicks, 7-day-old chicks were exposed to cold temperature (4 A degrees C) or kept warm (30 A degrees C). Chicks exposed to the cold showed cardiac hypertrophy with marked left ventricular (LV) chamber dilation and wall thickening. On the other hand, long-term cold exposure (2 weeks from 7-day-old) induced an increase in total ventricular mass, but not in LV morphological parameters. Then, we investigated the details of acute cardiac hypertrophy in chicks. Electron microscopy revealed that cardiomyocytes in the hypertrophied LV had enlarged mitochondria with less dense cristae. Although the mRNA expression of lipoprotein lipase in the LV of the cold-exposed chicks significantly increased, the mRNA expression of genes involved in fatty acid beta-oxidation did not change in response to cold exposure. In addition, the mRNA expression of peroxisome proliferator-activated receptor gamma coactivator-1 alpha, which enhances mitochondrial biogenesis and function under physiological cardiac hypertrophy, increased in LV of cold-exposed chicks. The study found that acute cold exposure to neonatal chicks induces LV hypertrophy. However, these results suggest that acute cold exposure to chicks might induce both adaptive and maladaptive responses of the LV.

    DOI: 10.1007/s00360-015-0957-x

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  • 鶏の骨格筋と脂肪の組織形成に対するβ<sub>2</sub>アドレナリン受容体シグナルの作用機序の解明

    Daichi Ijiri, Yoshitaka Ishimaru, Saki Shimamoto, Kanae Ishitani, Akira Ohtsuka

    Proceedings of Japanese Society for Animal Nutrition and Metabolism   59 ( 2 )   89 - 96   2015.10

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  • Single injection of the beta(2)-adrenergic receptor agonist, clenbuterol, into newly hatched chicks alters abdominal fat pad mass in growing birds Reviewed

    Yoshitaka Ishimaru, Daichi Ijiri, Said Shimamoto, Kanae Ishitani, Tsutomu Nojima, Akira Ohtsuka

    GENERAL AND COMPARATIVE ENDOCRINOLOGY   211   9 - 13   2015.1

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    Excessive energy is stored in white adipose tissue as triacylglycerols in birds as well as in mammals. Although beta(2)-adrenergic receptor agonists reduce adipose tissue mass in birds, the underlying mechanism remains unclear. The aim of the current study was to examine the effects of a single intraperitoneal injection of the beta(2)-adrenergic receptor agonist, clenbuterol, on the abdominal fat pad tissue development. Thirty-three chicks at 1-day-old were given a single intraperitoneal injection of clenbuterol (0.1 mg/kg body weight) or phosphate-buffered saline. At 2 weeks post-dose, the weight of the abdominal fat tissue was decreased in the clenbuterol-injected chicks, and small adipocyte-like cells were observed in the abdominal fat pad tissue of the clenbuterol-injected chicks. Then, the expression of mRNAs encoding genes related to avian adipogenesis was examined in the abdominal fat pat tissue. The expression of mRNAs encoding Kruppel-like zinc finger transcription factor 5 (KLF-5), KLF-15, and zinc finger protein 423 in the abdominal fat pad tissue of the clenbuterol-injected chicks was significantly lower (P &lt; 0.05) than that of the control chicks, while the expression of mRNA encoding peroxisome proliferator-activated receptor-gamma was not affected. In addition, both mRNA expression (P &lt; 0.05) and enzymatic activity (P &lt; 0.05) of fatty acid synthase (FAS) were decreased in the abdominal fat pad tissue of the clenbuterol-injected chicks, while clenbuterol injection did not affect FAS activity in liver. These results suggested that a single injection with clenbuterol into newly hatched chicks reduces their abdominal fat pad mass possibly via disrupting adipocyte development during later growth stages. (C) 2014 Elsevier Inc. All rights reserved.

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  • The effects of intraperitoneal clenbuterol injection on protein degradation and myostatin expression differ between the sartorius and pectoral muscles of neonatal chicks Reviewed

    Daichi Ijiri, Kanae Ishitani, Saki Shimamoto, Yoshitaka Ishimaru, Akira Ohtsuka

    GENERAL AND COMPARATIVE ENDOCRINOLOGY   206   111 - 117   2014.9

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    The purpose of this study was to investigate the effects of injection of the beta 2-adrenergic receptor agonist clenbuterol on the skeletal muscles of neonatal chicks (Gallus gallus domesticus). One-day-old chicks were randomly divided into four groups and given a single intraperitoneal injection of clenbuterol (0.01, 0.1, or 1 mg/kg) or phosphate-buffered saline. Twenty-four hours after the injection, the sartorius muscles (which consist of both slow- and fast-twitch fibers) of chicks that received 0.01 or 0.1 mg/kg clenbuterol were significantly heavier than those of controls, while there were no between-group differences in the weight of the pectoral muscles, which consist of only fast-twitch fibers. Muscle free N-t-methylhistidine, regarded as an index of myofibrillar proteolysis, was decreased in the sartorius muscle of the clenbuterol-injected chicks, while it was not affected in the pectoral muscles. In the sartorius muscle of the clenbuterol-injected chicks, myostatin and atrogin-1/MAFbx mRNA expressions were decreased, while in growth factor-I was unaffected. These observations suggested, in 1-day-old chicks, clenbuterol might increase mass of the sartorius muscle by decreasing myostatin gene expression and protein degradation. (C) 2014 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ygcen.2014.07.023

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MISC

  • ブロイラー初生雛への飼料給与の開始日齢が鶏肉に及ぼす影響 2)

    井尻大地, 宝蔵直樹, 島元紗希, 川口真奈, 古川愛理, 多田司, 友永省三, 中島一喜, 大塚彰

    鶏の研究   94 ( 2 )   16‐20   2019.2

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  • ブロイラー初生雛への飼料給与の開始日齢が鶏肉に及ぼす影響 1)

    井尻大地, 宝蔵直樹, 島元紗希, 川口真奈, 古川愛理, 多田司, 友永省三, 中島一喜, 大塚彰

    鶏の研究   94 ( 1 )   22‐26   2019.1

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Presentations

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Research Projects

  • 鶏肉品質を支配する要因の解明と品質の斉一性向上への取り組み

    Grant number:21K14958  2021.4 - 2024.3

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    島元 紗希

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    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

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  • 筋原線維タンパク質由来3-メチルヒスチジンのミオシン分解機構への影響

    Grant number:20K22609  2020.9 - 2022.3

    日本学術振興会  科学研究費助成事業 研究活動スタート支援  研究活動スタート支援

    島元 紗希

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    Grant amount:\2860000 ( Direct Cost: \2200000 、 Indirect Cost:\660000 )

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  • Studies on molecular mechanisms that regulate the skeletal muscle mass in meat type chickens.

    Grant number:17J02494  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows  Grant-in-Aid for JSPS Fellows

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    Grant amount:\2800000 ( Direct Cost: \2800000 )

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Teaching Experience

  • 統合化学入門

    2022
    Institution name:新潟大学

  • 動物栄養学

    2022
    Institution name:新潟大学

  • 食と健康の科学

    2021
    Institution name:新潟大学

  • 栄養生化学

    2021
    Institution name:新潟大学

  • 農学入門II

    2021
    Institution name:新潟大学

  • スタディ・スキルズAIa

    2021
    Institution name:新潟大学

  • スタディ・スキルズAIIa

    2021
    Institution name:新潟大学

  • 農学入門I

    2021
    Institution name:新潟大学

  • 生物学実験

    2020
    Institution name:新潟大学

  • 食品科学概論

    2020
    Institution name:新潟大学

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