2023/02/03 更新

写真a

ミヤモト タクジ
宮本 託志
MIYAMOTO Takuji
所属
日本酒学センター 特任助教
職名
特任助教
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学位

  • 博士(農学) ( 2015年10月   京都大学 )

  • 修士(農学) ( 2012年3月   京都大学 )

  • 学士(農学) ( 2010年3月   京都大学 )

経歴(researchmap)

  • 新潟大学   日本酒学センター   特任助教

    2021年2月 - 現在

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  • 京都大学   生存圏研究所 中核研究部 生存圏診断統御研究系 森林代謝機能化学分野   研究員

    2015年7月 - 2021年1月

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  • 京都大学   大学院農学研究科 応用生命科学専攻 植物栄養学分野   研究員

    2015年4月 - 2015年7月

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経歴

  • 新潟大学   日本酒学センター   特任助教

    2021年2月 - 現在

学歴

  • 京都大学   大学院農学研究科   応用生命科学専攻 植物栄養学分野 博士後期課程

    2012年4月 - 2015年3月

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  • 京都大学   大学院農学研究科   応用生命科学専攻 植物栄養学分野 修士課程

    2010年4月 - 2012年3月

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  • 京都大学   農学部   応用生命科学科

    2006年4月 - 2010年3月

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論文

  • Nitrogen fertilization of rice plants before flowering affects sake fermentation and quality 査読

    Takuji MIYAMOTO, Ikuhisa NISHIDA, Norikuni OHTAKE, Dai HIRATA

    Cereal Chemistry   2022年9月

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    担当区分:筆頭著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1002/cche.10603

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  • MYB-mediated regulation of lignin biosynthesis in grasses 招待 査読

    Takuji Miyamoto, Yuki Tobimatsu, Toshiaki Umezawa

    Current Plant Biology   24   100174 - 100174   2020年12月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.cpb.2020.100174

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  • Double knockout of OsWRKY36 and OsWRKY102 boosts lignification with altering culm morphology of rice 査読 国際誌

    Takuji Miyamoto, Rie Takada, Yuki Tobimatsu, Shiro Suzuki, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto, Toshiaki Umezawa

    Plant Science   296   110466 - 110466   2020年7月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    Breeding to enrich lignin, a major component of lignocelluloses, in plants contributes to enhanced applications of lignocellulosic biomass into solid biofuels and valuable aromatic chemicals. To collect information on enhancing lignin deposition in grass species, important lignocellulose feedstocks, we generated rice (Oryza sativa) transgenic lines deficient in OsWRKY36 and OsWRKY102, which encode putative transcriptional repressors for secondary cell wall formation. We used CRISPR/Cas9-mediated targeted mutagenesis and closely characterized their altered cell walls using chemical and nuclear magnetic resonance (NMR) methods. Both OsWRKY36 and OsWRKY102 mutations significantly increased lignin content by up to 28 % and 32 %, respectively. Additionally, OsWRKY36/OsWRKY102-double-mutant lines displayed lignin enrichment of cell walls (by up to 41 %) with substantially altered culm morphology over the single-mutant lines as well as the wild-type controls. Our chemical and NMR analyses showed that relative abundances of guaiacyl and p-coumarate units were slightly higher and lower, respectively, in the WRKY mutant lignins compared with those in the wild-type lignins. Our results provide evidence that both OsWRKY36 and OsWRKY102 are associated with repression of rice lignification.

    DOI: 10.1016/j.plantsci.2020.110466

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  • OsMYB108 loss‐of‐function enriches p‐coumaroylated and tricin lignin units in rice cell walls 査読 国際誌

    Takuji Miyamoto, Rie Takada, Yuki Tobimatsu, Yuri Takeda, Shiro Suzuki, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto, Toshiaki Umezawa

    The Plant Journal   98 ( 6 )   975 - 987   2019年6月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Breeding approaches to enrich lignins in biomass could be beneficial to improving the biorefinery process because lignins increase biomass heating value and represent a potent source of valuable aromatic chemicals. However, despite the fact that grasses are promising lignocellulose feedstocks, limited information is yet available for molecular-breeding approaches to upregulate lignin biosynthesis in grass species. In this study, we generated lignin-enriched transgenic rice (Oryza sativa), a model grass species, via targeted mutagenesis of the transcriptional repressor OsMYB108 using CRISPR/Cas9-mediated genome editing. The OsMYB108-knockout rice mutants displayed increased expressions of lignin biosynthetic genes and enhanced lignin deposition in culm cell walls. Chemical and two-dimensional nuclear magnetic resonance (NMR) analyses revealed that the mutant cell walls were preferentially enriched in γ-p-coumaroylated and tricin lignin units, both of which are typical and unique components in grass lignins. NMR analysis also showed that the relative abundances of major lignin linkage types were altered in the OsMYB108 mutants.

    DOI: 10.1111/tpj.14290

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.14290

  • Expression level of the sodium transporter gene OsHKT2;1 determines sodium accumulation of rice cultivars under potassium-deficient conditions 査読

    Takuji Miyamoto, Kumiko Ochiai, Yasunori Nonoue, Kazuki Matsubara, Masahiro Yano, Toru Matoh

    Soil Science and Plant Nutrition   61 ( 3 )   481 - 492   2015年5月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Informa UK Limited  

    DOI: 10.1080/00380768.2015.1005539

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  • Identification of quantitative trait loci associated with shoot sodium accumulation under low potassium conditions in rice plants 査読

    MIYAMOTO Takuji, OCHIAI Kumiko, TAKESHITA Saya, MATOH Toru

    Soil science and plant nutrition   58 ( 6 )   728 - 736   2012年12月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:一般社団法人日本土壌肥料学会  

    Sodium (Na) application has marked beneficial effects on plant growth when the potassium (K) supply is low. Under low K supply, three japonica rice (Oryza sativa L.) cultivars, Koshihikari, Nipponbare, and Sasanishiki, accumulated more Na than three indica cultivars, IR36, IR64, and Kasalath, and the effect of Na application on growth was greater in japonica Koshihikari plants than in indica IR64 plants. A quantitative trait locus (QTL) analysis using a population of backcross inbred lines derived from japonica Koshihikari and indica Kasalath identified two significant loci associated with shoot Na concentration on chromosomes 3 and 6. The quantitative trait locus for shoot Na accumulation on chromosome 6 was confirmed in a population of chromosome segment substitution lines. The major QTL detected in this study could be useful for increasing crop productivity under low K input.

    DOI: 10.1080/00380768.2012.745797

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  • Lignocellulose molecular assembly and deconstruction properties of lignin-altered rice mutants 査読

    Andri Fadillah Martin, Yuki Tobimatsu, Pui Ying Lam, Naoyuki Matsumoto, Takuto Tanaka, Shiro Suzuki, Ryosuke Kusumi, Takuji Miyamoto, Yuri Takeda-Kimura, Masaomi Yamamura, Taichi Koshiba, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto, Toshiaki Umezawa

    Plant Physiology   191 ( 1 )   70 - 86   2023年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    Abstract

    Bioengineering approaches to modify lignin content and structure in plant cell walls have shown promise for facilitating biochemical conversions of lignocellulosic biomass into valuable chemicals. Despite numerous research efforts, however, the effect of altered lignin chemistry on the supramolecular assembly of lignocellulose and consequently its deconstruction in lignin-modified transgenic and mutant plants is not fully understood. In this study, we aimed to close this gap by analyzing lignin-modified rice (Oryza sativa L.) mutants deficient in 5-HYDROXYCONIFERALDEHYDE O-METHYLTRANSFERASE (CAldOMT) and CINNAMYL ALCOHOL DEHYDROGENASE (CAD). A set of rice mutants harboring knockout mutations in either or both OsCAldOMT1 and OsCAD2 was generated in part by genome editing and subjected to comparative cell wall chemical and supramolecular structure analyses. In line with the proposed functions of CAldOMT and CAD in grass lignin biosynthesis, OsCAldOMT1-deficient mutant lines produced altered lignins depleted of syringyl and tricin units and incorporating noncanonical 5-hydroxyguaiacyl units, whereas OsCAD2-deficient mutant lines produced lignins incorporating noncanonical hydroxycinnamaldehyde-derived units. All tested OsCAldOMT1- and OsCAD2-deficient mutants, especially OsCAldOMT1-deficient lines, displayed enhanced cell wall saccharification efficiency. Solid-state nuclear magnetic resonance (NMR) and X-ray diffraction analyses of rice cell walls revealed that both OsCAldOMT1- and OsCAD2 deficiencies contributed to the disruptions of the cellulose crystalline network. Further, OsCAldOMT1 deficiency contributed to the increase of the cellulose molecular mobility more prominently than OsCAD2 deficiency, resulting in apparently more loosened lignocellulose molecular assembly. Such alterations in cell wall chemical and supramolecular structures may in part account for the variations of saccharification performance of the OsCAldOMT1- and OsCAD2-deficient rice mutants.

    DOI: 10.1093/plphys/kiac432

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    その他リンク: https://academic.oup.com/plphys/article-pdf/191/1/70/48449666/kiac432.pdf

  • Total lignin content and agromorphological character diversities of 30 Indonesian rice (Oryza sativa L.) accessions. 査読

    Widyajayantie D, Ardie SW, Purwoko BS, Miyamoto T, Umezawa T, Nugroho S

    Indonesian Journal of Biotechnology and Biosciences   9 ( 2 )   182 - 194   2022年12月

  • Physiology of microalgae and their application to sustainable agriculture: A mini-review 招待 査読

    Iffet Çakirsoy, Takuji Miyamoto, Norikuni Ohtake

    Frontiers in Plant Science   13   2022年11月

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    担当区分:責任著者  

    DOI: 10.3389/fpls.2022.1005991

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  • Genome-edited rice deficient in two <i>4-COUMARATE:COENZYME A LIGASE</i> genes displays diverse lignin alterations 査読

    Osama Ahmed Afifi, Yuki Tobimatsu, Pui Ying Lam, Andri Fadillah Martin, Takuji Miyamoto, Yuriko Osakabe, Keishi Osakabe, Toshiaki Umezawa

    Plant Physiology   190 ( 4 )   2155 - 2172   2022年9月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    Abstract

    The 4-coumarate:coenzyme A ligase (4CL) is a key enzyme that contributes to channeling metabolic flux in the cinnamate/monolignol pathway, leading to the production of monolignols, p-hydroxycinnamates, and a flavonoid tricin, the major building blocks of lignin polymer in grass cell walls. Vascular plants often contain multiple 4CL genes; however, the contribution of each 4CL isoform to lignin biosynthesis remains unclear, especially in grasses. In this study, we characterized the functions of two rice (Oryza sativa L.) 4CL isoforms (Os4CL3 and Os4CL4) primarily by analyzing the cell wall chemical structures of rice mutants generated by CRISPR/Cas9-mediated targeted mutagenesis. A series of chemical and nuclear magnetic resonance analyses revealed that loss-of-function of Os4CL3 and Os4CL4 differently altered the composition of lignin polymer units. Loss of function of Os4CL3 induced marked reductions in the major guaiacyl and syringyl lignin units derived from both the conserved non-γ-p-coumaroylated and the grass-specific γ-p-coumaroylated monolignols, with more prominent reductions in guaiacyl units than in syringyl units. By contrast, the loss-of-function mutation to Os4CL4 primarily decreased the abundance of the non-γ-p-coumaroylated guaiacyl units. Loss-of-function of Os4CL4, but not of Os4CL3, reduced the grass-specific lignin-bound tricin units, indicating that Os4CL4 plays a key role not only in monolignol biosynthesis but also in the biosynthesis of tricin used for lignification. Further, the loss-of-function of Os4CL3 and Os4CL4 notably reduced cell-wall-bound ferulates, indicating their roles in cell wall feruloylation. Overall, this study demonstrates the overlapping but divergent roles of 4CL isoforms during the coordinated production of various lignin monomers.

    DOI: 10.1093/plphys/kiac450

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  • Limiting silicon supply alters lignin content and structures of sorghum seedling cell walls 査読

    Reza Ramdan Rivai, Takuji Miyamoto, Tatsuya Awano, Arata Yoshinaga, Shuoye Chen, Junji Sugiyama, Yuki Tobimatsu, Toshiaki Umezawa, Masaru Kobayashi

    Plant Science   321   111325 - 111325   2022年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    Sorghum has been recognized as a promising energy crop. The composition and structure of lignin in the cell wall are important factors that affect the quality of plant biomass as a bioenergy feedstock. Silicon (Si) supply may affect the lignin content and structure, as both Si and lignin are possibly involved in plant mechanical strength. However, our understanding regarding the interaction between Si and lignin in sorghum is limited. Therefore, in this study, we analyzed the lignin in the cell walls of sorghum seedlings cultured hydroponically with or without Si supplementation. Limiting the Si supply significantly increased the thioglycolic acid lignin content and thioacidolysis-derived syringyl/guaiacyl monomer ratio. At least part of the modification may be attributable to the change in gene expression, as suggested by the upregulation of phenylpropanoid biosynthesis-related genes under-Si conditions. The cell walls of the-Si plants had a higher mechanical strength and calorific value than those of the +Si plants. These results provide some insights into the enhancement of the value of sorghum biomass as a feedstock for energy production by limiting Si uptake.

    DOI: 10.1016/j.plantsci.2022.111325

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  • Effects of improved sodium uptake ability on grain yields of rice plants under low potassium supply 査読

    Kumiko Ochiai, Kousuke Oba, Kanoko Oda, Takuji Miyamoto, Toru Matoh

    Plant Direct   6 ( 4 )   2022年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1002/pld3.387

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/pld3.387

  • Structural basis of lignocellulose deconstruction by the wood-feeding anobiid beetle Nicobium hirtum 査読

    Ni Putu Ratna Ayu Krishanti, Yuki Tobimatsu, Takuji Miyamoto, Izumi Fujimoto, Titik Kartika, Toshiaki Umezawa, Toshimitsu Hata, Tsuyoshi Yoshimura

    Journal of Wood Science   68 ( 1 )   2022年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    Abstract

    The details of the lignocellulose deconstruction processes in the digestive systems of wood-feeding insects remain elusive. This study aimed to examine the biochemical conversion of lignocellulose in the digestive system of a wood-feeding anobiid beetle, Nicobium hirtum, one of the most important pests of wooden products in Japan. To this end, N. hirtum larvae were fed with Japanese red pine (softwood) and Japanese beech (hardwood) sapwood diets, as well as an artificial diet containing Shorea wood (hardwood) sapwood sawdust. The structural differences between the original and digested (feces) lignocellulose samples were examined using wet-chemical and two-dimensional (2D) nuclear magnetic resonance (NMR) methods. Cellulose and hemicelluloses, especially mannan in the softwood diet, were preferentially degraded over lignin in the larval digestive system. As a result, lignin was enriched in the digested lignocellulose residues. Lignin compositional analyses based on thioacidolysis and 2D NMR determined that the proportions of oxidized lignin aromatic units were notably increased after digestion. Further, the 2D NMR analyses revealed the accumulation of aldehyde and hydroxypropiovanillone/syringone end-unit structures in lignin, indicating that oxidative and/or reductive modifications of lignin polymers occur in the larval digestive system. Such structural alterations of lignin may facilitate the dissociation of the lignin barrier, thereby liberating polysaccharides for subsequent enzymatic conversion for assimilation and energy.

    DOI: 10.1186/s10086-022-02017-6

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    その他リンク: https://link.springer.com/article/10.1186/s10086-022-02017-6/fulltext.html

  • Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings 査読

    Reza Ramdan Rivai, Takuji Miyamoto, Tatsuya Awano, Rie Takada, Yuki Tobimatsu, Toshiaki Umezawa, Masaru Kobayashi

    Scientific Reports   11 ( 1 )   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    <title>Abstract</title>Sorghum [<italic>Sorghum bicolor</italic> (L.) Moench] has been gaining attention as a feedstock for biomass energy production. While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components. Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls. The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods. We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings. Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-<sc>d</sc>-glucan, and arabinoxylan. At least some of these cell wall alterations could be associated with changes in gene expression. Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.

    DOI: 10.1038/s41598-021-02570-y

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    その他リンク: https://www.nature.com/articles/s41598-021-02570-y

  • A microscale protocol for alkaline nitrobenzene oxidation of lignins using an easily available reactor. 査読

    Masaomi Yamamura, Takuji Miyamoto, Rie Takada, Dwi Widyajayantie, Vincentia Esti Windiastri, Satya Nugroho, Toshiaki Umezawa

    Lignin   2   19 - 24   2021年7月

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    掲載種別:研究論文(学術雑誌)  

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  • Effects of adding ammonium dihydrogen phosphate to a water-soluble extract of the inner part of oil palm trunk on binderless particleboard. 査読

    Rahma Nur Komariah, Takuji Miyamoto, Sukma Surya Kusumah, Soichi Tanaka, Toshiaki Umezawa, Kozo Kanayama, Kenji Umemura

    Bioresources   16 ( 3 )   6015 - 6030   2021年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:BioResources  

    This study investigated the effects of adding ammonium dihydrogen phosphate (ADP) on the physical and chemical changes of a water-soluble extract of the inner part of oil palm trunk (OPT) to clarify the bonding mechanism of the binderless particleboard. The extract’s effect on ADP-added binderless particleboard was also investigated. OPT particles were treated by hot water at 60 °C for 6 h. Water-soluble extract and treated OPT particles were obtained. ADP was added to the water-soluble extract at 0, 10, and 40 wt%, and the mixtures were heated at 180 °C for 10 min. Furthermore, binderless particleboards using the treated particles were manufactured with similar condition. The 10 wt% ADP mixture changed the water-soluble extract to an insoluble substance, which was twice that of with 0 wt.% ADP addition. Infrared spectroscopy revealed peaks of furan and carbonyl in the insoluble substance. This indicated that the free sugar content in the water-soluble extract would change to furan compounds. Thermal analysis revealed that the resulting insoluble substance had good thermal stability, suggesting a high-molecular-weight substance. The insoluble substance would contribute to bonding of the binderless particleboards. In particular, a significant contribution to the water resistance was observed.

    DOI: 10.15376/biores.16.3.6015-6030

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  • Examination of the usability of leaf chlorophyll content and gene expression analyses as nitrogen status biomarkers in Sorghum bicolor 査読

    Reza Ramdan Rivai, Rie Takada, Takuji Miyamoto, Shigeru Hanano, Daisuke Shibata, Katsuaki Ohdoi, Masaru Kobayashi

    Journal of Plant Nutrition   44 ( 6 )   773 - 790   2021年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Informa UK Limited  

    Sorghum [Sorghum bicolor (L.) Moench] has been receiving attention as a feedstock for biomass energy. Since nitrogen (N) significantly affects the plant biomass production, diagnosing the N status of sorghum during its growth would be significant. Therefore, in this study, we analyzed the changes in several physiological parameters of sorghum in response to N limitation, with the aim of identifying candidates of N status biomarkers in this species. Parameters responding early and sensitively to N deficiency were searched in hydroponically cultured seedlings, and their applicability to field conditions was examined. We found that the leaf chlorophyll content measured as a soil-plant analysis development (SPAD) value was promising as a candidate N status biomarker, as it showed responses consistent with N regimes in field conditions in multiple genotypes. A gene expression analysis was capable of detecting N deficiency sensitively at an early stage, although its application to practical situations requires further investigation to identify a suitable sets of marker genes.

    DOI: 10.1080/01904167.2020.1867581

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  • Lignin metabolic engineering in grasses for primary lignin valorization. 査読

    Toshiaki Umezawa, Yuki Tobimatsu, Masaomi Yamamura, Takuji Miyamoto, Yuri Takeda, Taichi Koshiba, Rie Takada, Pui Ying Lam, Shiro Suzuki, Masahiro Sakamoto

    Lignin   1   30 - 41   2020年9月

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    掲載種別:研究論文(学術雑誌)  

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  • Variation in lignocellulose characteristics of 30 Indonesian sorghum (Sorghum bicolor) accessions 査読

    Y. Wahyuni, T. Miyamoto, H. Hartati, D. Widjayantie, V.E. Windiastri, Y. Sulistyowati, A. Rachmat, N.S. Hartati, S.K. Ragamustari, Y. Tobimatsu, S. Nugroho, T. Umezawa

    Industrial Crops and Products   142   111840 - 111840   2019年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.indcrop.2019.111840

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  • High-performance binderless particleboard from the inner part of oil palm trunk by addition of ammonium dihydrogen phosphate 査読

    Rahma Nur Komariah, Takuji Miyamoto, Soichi Tanaka, Kurnia Wiji Prasetiyo, Firda Aulya Syamani, Subyakto, Toshiaki Umezawa, Kozo Kanayama, Kenji Umemura

    Industrial Crops and Products   141   111761 - 111761   2019年12月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.indcrop.2019.111761

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  • Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice 査読 国際誌

    Andri Fadillah Martin, Yuki Tobimatsu, Ryosuke Kusumi, Naoyuki Matsumoto, Takuji Miyamoto, Pui Ying Lam, Masaomi Yamamura, Taichi Koshiba, Masahiro Sakamoto, Toshiaki Umezawa

    Scientific Reports   9 ( 1 )   17153 - 17153   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    <title>Abstract</title>Lignin is a complex phenylpropanoid polymer deposited in plant cell walls. Lignin has long been recognized as an important limiting factor for the polysaccharide-oriented biomass utilizations. To mitigate lignin-associated biomass recalcitrance, numerous mutants and transgenic plants that produce lignocellulose with reduced lignin contents and/or lignins with altered chemical structures have been produced and characterised. However, it is not fully understood how altered lignin chemistry affects the supramolecular structure of lignocellulose, and consequently, its utilization properties. Herein, we conducted comprehensive chemical and supramolecular structural analyses of lignocellulose produced by a rice <italic>cad2</italic> mutant deficient in <italic>CINNAMYL ALCOHOL DEHYDROGENASE</italic> (<italic>CAD</italic>), which encodes a key enzyme in lignin biosynthesis. By using a solution-state two-dimensional NMR approach and complementary chemical methods, we elucidated the structural details of the altered lignins enriched with unusual hydroxycinnamaldehyde-derived substructures produced by the <italic>cad2</italic> mutant. In parallel, polysaccharide assembly and the molecular mobility of lignocellulose were investigated by solid-state <sup>13</sup>C MAS NMR, nuclear magnetic relaxation, X-ray diffraction, and Simon’s staining analyses. Possible links between <italic>CAD</italic>-associated lignin modifications (in terms of total content and chemical structures) and changes to the lignocellulose supramolecular structure are discussed in the context of the improved biomass saccharification efficiency of the <italic>cad2</italic> rice mutant.

    DOI: 10.1038/s41598-019-53156-8

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    その他リンク: http://www.nature.com/articles/s41598-019-53156-8

  • NMR studies on lignocellulose deconstructions in the digestive system of the lower termite Coptotermes formosanus Shiraki 査読

    Didi Tarmadi, Yuki Tobimatsu, Masaomi Yamamura, Takuji Miyamoto, Yasuyuki Miyagawa, Toshiaki Umezawa, Tsuyoshi Yoshimura

    Scientific Reports   8 ( 1 )   1290   2018年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1038/s41598-018-19562-0

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    その他リンク: http://www.nature.com/articles/s41598-018-19562-0.pdf

  • Comparative analysis of lignin chemical structures of sugarcane bagasse pretreated by alkaline, hydrothermal, and dilute sulfuric acid methods 査読

    Takuji Miyamoto, Asako Mihashi, Masaomi Yamamura, Yuki Tobimatsu, Shiro Suzuki, Rie Takada, Yoshinori Kobayashi, Toshiaki Umezawa

    Industrial Crops and Products   121   124 - 131   2018年10月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.indcrop.2018.04.077

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  • A comparative study of the biomass properties of Erianthus and sugarcane: lignocellulose structure, alkaline delignification rate, and enzymatic saccharification efficiency 査読

    Takuji Miyamoto, Masaomi Yamamura, Yuki Tobimatsu, Shiro Suzuki, Miho Kojima, Keiji Takabe, Yoshifumi Terajima, Asako Mihashi, Yoshinori Kobayashi, Toshiaki Umezawa

    Bioscience, Biotechnology, and Biochemistry   82 ( 7 )   1143 - 1152   2018年7月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    <title>Abstract</title>
    A comprehensive understanding of the structure and properties of gramineous lignocelluloses is needed to facilitate their uses in biorefinery. In this study, lignocelluloses from fractionated internode tissues of two taxonomically close species, Erianthus arundinaceus and sugarcane (Saccharum spp.), were characterized. Our analyses determined that syringyl (S) lignins were predominant over guaiacyl (G) or p-hydroxyphenyl (H) lignins in sugarcane tissues; on the other hand, S lignin levels were similar to those of G lignin in Erianthus tissues. In addition, tricin units were detected in sugarcane tissues, but not in Erianthus tissues. Distributions of lignin inter-monomeric linkage types were also different in Erianthus and sugarcane tissues. Alkaline treatment removed lignins from sugarcane tissues more efficiently than Erianthus tissues, resulting in a higher enzymatic digestibility of sugarcane tissues compared with Erianthus tissues. Our data indicate that Erianthus biomass displayed resistance to alkaline delignification and enzymatic digestion.

    DOI: 10.1080/09168451.2018.1447358

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  • The effects of various lignocelluloses and lignins on physiological responses of a lower termite, Coptotermes formosanus 査読

    Didi Tarmadi, Tsuyoshi Yoshimura, Yuki Tobimatsu, Masaomi Yamamura, Takuji Miyamoto, Yasuyuki Miyagawa, Toshiaki Umezawa

    Journal of Wood Science   63 ( 5 )   464 - 472   2017年10月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s10086-017-1638-z

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    その他リンク: http://link.springer.com/content/pdf/10.1007/s10086-017-1638-z.pdf

▶ 全件表示

書籍等出版物

  • 日本酒学講義

    新潟大学日本酒学センター( 担当: 分担執筆 ,  範囲: コラム3 米作りから見る日本酒学)

    ミネルヴァ書房  2022年4月  ( ISBN:9784623093182

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    総ページ数:x, 306, 5p   記述言語:日本語

    CiNii Books

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  • 米の機能性食品化と新規利用技術・高度加工技術の開発-食糧,食品素材,機能性食品,工業原料,医薬品原料としての米-

    ( 担当: 分担執筆 ,  範囲: 第3章 第8節 第3項「米作りと酒造りのつながり -米由来の日本酒成分に着目した考察-」)

    テクノシステム  2022年3月  ( ISBN:9784924728929

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担当経験のある授業科目

  • 日本酒学A

    2022年
    -
    現在
    機関名:新潟大学

  • 日本酒学C

    2021年
    -
    現在
    機関名:新潟大学

  • 日本酒学B

    2021年
    -
    現在
    機関名:新潟大学

  • 日本酒学A-2

    2021年
    機関名:新潟大学

  • 日本酒学A-1

    2021年
    機関名:新潟大学