Updated on 2022/12/01

写真a

 
HARADA Naoki
 
Organization
Academic Assembly Institute of Science and Technology NOUGAKU KEIRETSU Professor
Faculty of Agriculture Professor
Title
Professor
Other name(s)
Niigata University
External link

Degree

  • 博士(農学) ( 2001.3   東京大学 )

Research Areas

  • Environmental Science/Agriculture Science / Landscape science

  • Environmental Science/Agriculture Science / Environmental load reduction and remediation

  • Environmental Science/Agriculture Science / Environmental agriculture

  • Life Science / Plant nutrition and soil science

Research History (researchmap)

  • Niigata University   Graduate School of Science and Technology Specialized Course of Life and Food Sciences

    2018.4

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  • Niigata University   Faculty of Agriculture Department of Agriculture   Professor

    2018.4

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  • Niigata University   Faculty of Agriculture Department of Agriculture   Associate Professor

    2017.4 - 2018.3

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  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2007.4 - 2018.3

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  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry   Associate Professor

    2007.4 - 2017.3

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Research History

  • Niigata University   Faculty of Agriculture   Professor

    2018.4

  • Niigata University   Faculty of Agriculture Department of Agriculture   Associate Professor

    2017.4 - 2018.3

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2007.4 - 2018.3

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2007.4 - 2018.3

  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry   Associate Professor

    2007.4 - 2017.3

Professional Memberships

 

Papers

  • Improvement in potassium budget with water outflow reduction through reducing excessive irrigation in a paddy field Reviewed

    Tatsuhiro Nishikiori, Tomijiro Kubota, Susumu Miyazu, Naoki Harada, Natsuki Yoshikawa

    SOIL SCIENCE AND PLANT NUTRITION   2022.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    Countermeasures to reduce radiocesium uptake by crops have been implemented in farmlands affected by the accident at Tokyo Electric Power Company's Fukushima Daiichi Nuclear Power Plant in 2011. A widely practiced countermeasure is the application of K. Long-term maintenance of soil K is crucial due to the long physical half-life of Cs-137 (30 years). Plant-available K budgets can be used as an indication of sustainable maintenance. Our previous study found that large water outflow, especially percolation, associated with excessive irrigation caused major K outflow and a negative K budget in a paddy field in Fukushima in 2018. The present study evaluated whether decreasing the water outflow by reducing the irrigation volume from 6300 to 1900 mm reduced K outflow and improved the K budget in the field during the cropping period in 2019. K concentrations in soil solution were comparable in both years, and the percolation water volume decreased to 37% that of 2018, resulting in a 53% reduction in the K output from 392 to 184 kg ha(-1). The input via irrigation also decreased (-35 kg ha(-1)), but the output via surface runoff showed a greater reduction (-58 kg ha(-1)) owing to a 70% decrease in the water volume compared with 2018. Consequently, the K budget was substantially improved (from -289 to -57 kg ha(-1)), highlighting the importance of controlling water outflow, particularly percolation, for soil K maintenance in paddy fields with high permeability.

    DOI: 10.1080/00380768.2022.2108685

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  • Investigation on the radiocesium transfer to rice plants near the water inlet of paddy fields via an in situ experiment using non-contaminated soil Reviewed

    Anastasiia Klevtsova, Rinji Inaba, Moeka Takahashi, Yoshimasa Suzuki, Susumu Miyazu, Kazuki Suzuki, Naoki Harada, Norio Nogawa, Tatsuhiro Nishikiori, Tomijiro Kubota, Natsuki Yoshikawa

    JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY   2022.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    An in situ model paddy field experiment was conducted using non-contaminated soil to identify factors that locally increase the radiocesium activity concentrations in rice near the water inlet of paddy fields. The results demonstrated that irrigation water intake reduced exchangeable potassium concentration and increased radiocesium activity concentration in the soil near the water inlet; thus, the radiocesium activity concentration in rice was negatively correlated with the former and positively with the latter. Exchangeable potassium flushing and deposition of suspended solids in the soil owing to irrigation water intake facilitated the transfer of radiocesium to rice plants near the water inlet.

    DOI: 10.1007/s10967-022-08448-1

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  • Elevated rice 137Cs concentrations near the water inlet in paddy fields after the Fukushima nuclear accident Reviewed

    Yoshimasa Suzuki, Ryosuke Shoji, Takahiro Tsurumaki, Syohei Tamaki, Kousei Nakashima, Susumu Miyazu, Natsuki Yoshikawa, Hideki Ishii, Norio Nogawa, Masanori Nonaka, Kazuki Suzuki, Naoki Harada

    Journal of Radioanalytical and Nuclear Chemistry   331 ( 7 )   3167 - 3173   2022.7

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10967-022-08350-w

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    Other Link: https://link.springer.com/article/10.1007/s10967-022-08350-w/fulltext.html

  • Impact of the chemical composition of applied organic materials on bacterial and archaeal community compositions in paddy soil Reviewed

    Sharmin Akter Chowdhury, Aya Kaneko, Md Zakaria Ibne Baki, Chikako Takasugi, Natsumi Wada, Rasit Asiloglu, Naoki Harada, Kazuki Suzuki

    Biology and Fertility of Soils   58 ( 2 )   135 - 148   2022.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00374-022-01619-y

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    Other Link: https://link.springer.com/article/10.1007/s00374-022-01619-y/fulltext.html

  • Suppressive effect of the deep placement of lime nitrogen on N2O emissions in a soybean field Reviewed

    Hiromu Ikezawa, Yoshifumi Nagumo, Makoto Hattori, Masanori Nonaka, Takuji Ohyama, Naoki Harada

    Science of The Total Environment   804   150246 - 150246   2022.1

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.scitotenv.2021.150246

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  • Soil-root interface influences the assembly of the endophytic bacterial community in rice plants Reviewed

    Solomon Oloruntoba Samuel, Kazuki Suzuki, Rasit Asiloglu, Naoki Harada

    Biology and Fertility of Soils   2021.11

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00374-021-01611-y

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    Other Link: https://link.springer.com/article/10.1007/s00374-021-01611-y/fulltext.html

  • Soil properties have more significant effects on the community composition of protists than the rhizosphere effect of rice plants in alkaline paddy field soils Reviewed

    Rasit Asiloglu, Keiko Shiroishi, Kazuki Suzuki, Oguz Can Turgay, Naoki Harada

    Soil Biology and Biochemistry   161   108397 - 108397   2021.10

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.soilbio.2021.108397

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  • Molecular Genetic Characterization of Arbuscular Mycorrhizal Fungi Associated with Upland Rice in Bangladesh Reviewed

    Md Zakaria Ibne Baki, Kazuki Suzuki, Kohei Takahashi, Sharmin Akter Chowdhury, Rasit Asiloglu, Naoki Harada

    Rhizosphere   18   100357 - 100357   2021.6

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    DOI: 10.1016/j.rhisph.2021.100357

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  • Top-down effects of protists are greater than bottom-up effects of fertilisers on the formation of bacterial communities in a paddy field soil Reviewed

    Rasit Asiloglu, Kobayashi Kenya, Solomon Oloruntoba Samuel, Bahar Sevilir, Jun Murase, Kazuki Suzuki, Naoki Harada

    Soil Biology and Biochemistry   156   108186 - 108186   2021.5

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.soilbio.2021.108186

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  • Effect of protists on rhizobacterial community composition and rice plant growth in a biochar amended soil Reviewed

    Rasit Asiloglu, Bahar Sevilir, Solomon Oloruntoba Samuel, Murat Aycan, Muhittin Onur Akca, Kazuki Suzuki, Jun Murase, Oguz Can Turgay, Naoki Harada

    Biology and Fertility of Soils   57 ( 2 )   293 - 304   2021.2

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00374-020-01525-1

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    Other Link: http://link.springer.com/article/10.1007/s00374-020-01525-1/fulltext.html

  • Impacts of application of calcium cyanamide and the consequent increase in soil pH on N2O emissions and soil bacterial community compositions Reviewed

    Kazuki Suzuki, Naoya Kashiwa, Kota Nomura, Rasit Asiloglu, Naoki Harada

    Biology and Fertility of Soils   57 ( 2 )   269 - 279   2021.2

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00374-020-01523-3

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    Other Link: http://link.springer.com/article/10.1007/s00374-020-01523-3/fulltext.html

  • Biochar affects taxonomic and functional community composition of protists Reviewed

    Rasit Asiloglu, Solomon Oloruntoba Samuel, Bahar Sevilir, Muhittin Onur Akca, Pinar Acar Bozkurt, Kazuki Suzuki, Jun Murase, Oguz Can Turgay, Naoki Harada

    Biology and Fertility of Soils   57 ( 1 )   15 - 29   2021.1

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    DOI: 10.1007/s00374-020-01502-8

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    Other Link: http://link.springer.com/article/10.1007/s00374-020-01502-8/fulltext.html

  • Influence of irrigation water intake on local increase of radiocesium activity concentration in rice plants near a water inlet Reviewed International journal

    Natsuki Yoshikawa, Kosei Nakashima, Yoshimasa Suzuki, Susumu Miyazu, Kazuki Suzuki, Norio Nogawa, Naoki Harada

    Journal of Environmental Radioactivity   225   106441 - 106441   2020.12

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    To identify the cause of the phenomenon that rice plants close to the water inlet contain relatively higher radiocesium within a paddy field plot, we conducted a field experiment by establishing experimental channel using polypropylene corrugated sheets, and sampling surface water, paddy soil and rice plants according to the distance from the water inlet in 2014 and 2015. It was found that the 137Cs activity concentrations in both dissolved and particulate forms in paddy surface water presented a declining trend from the water inlet towards the outlet. The 137Cs activity concentration in paddy soil in the harvesting season and those of brown rice and rice straws were highest at 1-2 m from the water inlet. Balance calculation suggests that destination of the lost 137Cs from the surface water was likely to be adsorption of the dissolved form and sedimentation of particulate form onto the soil. The concentration of exchangeable potassium ion in paddy soil was below the recommended standard of 250 mg kg-1 (as K2O in dry soil) near the water inlet at the harvesting period both years.These findings suggested that the possible crucial factors to induce rice plant uptake of radiocesium near the water inlet were either (1) direct absorption of dissolved 137Cs in surface water by rice plants, (2) absorption of 137Cs, which was originally retained in particulate matter and released by ion exchange and/or by organic matter decomposition in combination with (3) loss of soil exchangeable potassium caused due partly to transportation of soil particles with exchangeable potassium by the rapid water flow near the water inlet and/or leaching by ion exchange onto the soil of other cations such as calcium ion flowing into the paddy field. These findings will contribute to providing possible measures for producing safe rice in highly contaminated areas in which agricultural production will resume in the near future. We propose providing a non-planting zone for the area closer than about 5 m from the water intake to avoid the occurrence of high 137Cs concentrations in rice crops.

    DOI: 10.1016/j.jenvrad.2020.106441

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  • Protist-enhanced survival of a plant growth promoting rhizobacteria, Azospirillum sp. B510, and the growth of rice (Oryza sativa L.) plants Reviewed

    Asiloglu R, Shiroishi K, Suzuki K, Turgay OC, Murase J, Harada N

    Applied Soil Ecology   154   103599 - 103599   2020.10

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    DOI: 10.1016/j.apsoil.2020.103599

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  • The composition characteristics of arbuscular mycorrhizal fungal communities associated with barley in saline-alkaline soils in Central Anatolia Reviewed

    Kaidzu T, Suzuki K, Sugiyama H, Akca MO, Ergül A, Turgay OC, Nonaka M, Harada N

    Soil Science and Plant Nutrition   66 ( 2 )   268 - 274   2020.4

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  • Input and output pathways determining potassium budgets of two paddy fields implementing countermeasures against radiocesium in Fukushima, Japan Reviewed

    Nishikiori T, Kubota T, Miyazu S, Harada N, Yoshikawa N, Fujiwara H, Saito T

    Plos one   15 ( 4 )   e0232139   2020.4

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  • Molecular characterization, mycelial compatibility grouping, and aggressiveness of a newly emerging phytopathogen, Sclerotinia sclerotiorum, causing white mold disease in new host crops in Bangladesh Reviewed

    Rahman MME, Suzuki K, Islam MM, Dey TK, Harada N, Hossain DM

    Journal of Plant Pathology   102 ( 3 )   775 - 785   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s42161-020-00503-8

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    Other Link: http://link.springer.com/article/10.1007/s42161-020-00503-8/fulltext.html

  • Evaluation of primer pairs for studying arbuscular mycorrhizal fungal community compositions using an MiSeq platform Reviewed

    Suzuki K, Takahashi K, Harada N

    Biology Fertility of Soils   2020.4

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  • Salt tolerance improvement in rice through efficient SNP marker-assisted selection coupled with speed-breeding Reviewed International journal

    Md Masud Rana, Takeshi Takamatsu, Marouane Baslam, Kentaro Kaneko, Kimiko Itoh, Naoki Harada, Toshie Sugiyama, Takayuki Ohnishi, Tetsu Kinoshita, Hiroki Takagi, Toshiaki Mitsui

    International journal of molecular sciences   20 ( 10 )   2585   2019.5

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    Salinity critically limits rice metabolism, growth, and productivity worldwide. Improvement of the salt resistance of locally grown high-yielding cultivars is a slow process. The objective of this study was to develop a new salt-tolerant rice germplasm using speed-breeding. Here, we precisely introgressed the hst1 gene, transferring salinity tolerance from "Kaijin" into high-yielding "Yukinko-mai" (WT) rice through single nucleotide polymorphism (SNP) marker-assisted selection. Using a biotron speed-breeding technique, we developed a BC3F3 population, named "YNU31-2-4", in six generations and 17 months. High-resolution genotyping by whole-genome sequencing revealed that the BC3F2 genome had 93.5% similarity to the WT and fixed only 2.7% of donor parent alleles. Functional annotation of BC3F2 variants along with field assessment data indicated that "YNU31-2-4" plants carrying the hst1 gene had similar agronomic traits to the WT under normal growth condition. "YNU31-2-4" seedlings subjected to salt stress (125 mM NaCl) had a significantly higher survival rate and increased shoot and root biomasses than the WT. At the tissue level, quantitative and electron probe microanalyzer studies indicated that "YNU31-2-4" seedlings avoided Na+ accumulation in shoots under salt stress. The "YNU31-2-4" plants showed an improved phenotype with significantly higher net CO2 assimilation and lower yield decline than WT under salt stress at the reproductive stage. "YNU31-2-4" is a potential candidate for a new rice cultivar that is highly tolerant to salt stress at the seedling and reproductive stages, and which might maintain yields under a changing global climate.

    DOI: 10.3390/ijms20102585

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  • Differences in soil bacterial community compositions in paddy fields under organic and conventional farming conditions Reviewed

    Kazuki Suzuki, Manami Takemura, Takaaki Miki, Masanori Nonaka, Naoki Harada

    Microbes and Environments   34 ( 1 )   108 - 111   2019.3

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    Soil bacterial community compositions and temporal changes in organic paddy fields were elucidated using a 16S rRNA gene amplicon sequencing analysis with a high-throughput next generation sequencer. At transplanting, bacterial community compositions in organic and conventional paddy fields were mostly similar despite differences in field management. The bacterial community composition in organic fields differed from that under conventional management during the rice growth period, possibly as a result of the decomposition process of organic fertilizers. However, differences in the frequency of tillage and photosynthetic bacterial inoculations in the organic plots had less of an impact on bacterial communities.

    DOI: 10.1264/jsme2.ME18101

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  • Yearly changes in rice and soil radiocaesium concentrations in Minamisoma City, Fukushima Prefecture, Japan —Monitoring results from 2013 to 2016— Reviewed

    Suzuki Y, Shoji R, Tsurumaki T, Matsubara T, Tamaki S, Nakajima K, Tsuruta R, Yoshikawa N, Ishii H, Nogawa N, Nonaka M, Harada N

    Radioisotopes   68 ( 1 )   1 - 12   2019.1

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  • Biological control of tomato collar rot induced by Sclerotium rolfsii using Trichoderma species isolated in Bangladesh Reviewed

    Md. Monirul Islam, Delwar M. Hossain, Masanori Nonaka, Naoki Harada

    Archives of Phytopathology and Plant Protection   50 ( 3-4 )   109 - 116   2017.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Taylor and Francis Ltd.  

    This study evaluated three Trichoderma strains (T. harzianum TR05, T. virens TR06 and T. asperellum TR08) originating from Bangladesh as potential biological control agents against collar rot of tomato under greenhouse conditions. After seed treatment with TR05, a disease incidence of collar rot (5.36%) was lower than for TR06 (34.2%) and TR08 (20.8%). Germination percentage of tomato was highest for TR05 (90.3%). In soil treatment, inoculation with TR08 resulted in the lowest disease incidence (9.78%), and the disease incidence was statistically no different from that for TR05 (16.4%). Thus, TR05 and TR08 have potential as biological control agents of collar rot in tomato.

    DOI: 10.1080/03235408.2016.1265243

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  • Measurement and estimation of radiocesium discharge rate from paddy field during land preparation and mid-summer drainage Reviewed

    Susumu Miyazu, Tetsuo Yasutaka, Natsuki Yoshikawa, Shouhei Tamaki, Kousei Nakajima, Iku Sato, Masanori Nonaka, Naoki Harada

    JOURNAL OF ENVIRONMENTAL RADIOACTIVITY   155   23 - 30   2016.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCI LTD  

    In this research, we evaluated the range of Cs-137 discharge rates from paddy fields during land preparation and mid-summer drainage. First, we investigated Cs-137 discharge loads during land preparation and mid-summer drainage and their ratio to the Cs-137 inventory of paddy field soil. We found that total discharge rates were 0.003-0.028% during land preparation and 0.001-0.011% during mid-summer drainage. Next, we validated the range of obtained total discharge of Cs-137 from the paddy fields using a simplified equation and literature review. As a result, we conclude that the range of total outflow loads of suspended solids for the investigated paddy field was generally representative of paddy fields in Japan. Moreover, the Cs-137 discharge ratio had a wide range, but was extremely small relative to Cs-137 present in paddy field soil before irrigation. (C) 2016 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jenvrad.2016.01.013

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  • 4,4'-DDE and Endosulfan Levels in Agricultural Soils of the Cukurova Region, Mediterranean Turkey Reviewed

    Muhittin Onur Akca, Shihoko Hisatomi, Manami Takemura, Naoki Harada, Masanori Nonaka, Futa Sakakibara, Kazuhiro Takagi, Oguz Can Turgay

    BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY   96 ( 3 )   376 - 382   2016.3

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    Mediterranean Turkey has long been at the forefront of Turkish agriculture and the use of organochlorinated pesticides (OCPs) in this area rose considerably between the 1940s and 1980s. This study aimed to determine OCP residue levels in agricultural soils collected from the Mersin and Adana Districts, Cukurova Basin in Mediterranean Turkey. Most soil samples were contaminated with one, or both, of two OCP metabolites; 4,4'-dichlorodiphenyldichloroethylene (4,4'-DDE) and endosulfan sulfate. 4,4'-DDE occurred in 27 of the 29 samples and ranged from 6 to 1090 A mu g kg(-1)-dry soil (ds)(-1), while six samples contained endosulfan sulfate ranging between 82 and 1226 A mu g kg(-1)-ds(-1). Generally, horticultural and corn-planted soils contained only 4,4'-DDE, whereas greenhouse cultivation appeared to accumulate both residues. This study indicated that 4,4'-DDE occurred above acceptable levels of risk in agricultural soils of Mersin District and further studies on the qualitative and quantitative assessment of OCPs in other agricultural regions with intensive pesticide use are necessary to fully understand the impact of OCPs on agricultural soil in Turkey.

    DOI: 10.1007/s00128-015-1714-2

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  • Native Trichoderma strains isolated from Bangladesh with broad spectrum antifungal action against fungal phytopathogens Reviewed

    Md. Monirul Islam, Delwar M. Hossain, Md. Muzahid E Rahman, Kazuki Suzuki, Taishi Narisawa, Ismail Hossain, Md. Bahadur Meah, Masanori Nonaka, Naoki Harada

    Archives of Phytopathology and Plant Protection   49 ( 1-4 )   75 - 93   2016.2

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    Nineteen Trichoderma isolates, collected from different locations in Bangladesh, were characterised through phenotypic, biochemical and molecular means. Besides, they were assessed for their antifungal action in vitro. The isolates were divided into three groups: T. asperellum, T. virens and T. harzianum. A dual culture assay and a culture filtrate assay against 6 phytopathogens revealed that 9 of the 19 isolates showed significant antifungal activities. The isolate T. harzianum TR05 showed the highest inhibition against Fusarium oxysporum, Rhizoctonia solani, Fusarium circinatum and Phomopsis vexans, followed by T. asperellum TR08 and T. virens TR06. TR08 had the highest inhibition against Sclerotium rolfsii and Pythium aphanidermatum, followed by TR05 and TR06. These findings were in agreement with their activities of extracellular hydrolytic enzymes, including chitinase, β-1,3-glucanase, and proteinase. Our results suggest that isolates TR05, TR06 and TR08 have the potential to be effective biocontrol agents against the phytopathogenic fungi.

    DOI: 10.1080/03235408.2016.1147121

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  • Suppressive effects of Bacillus spp. on mycelia, apothecia and sclerotia formation of Sclerotinia sclerotiorum and potential as biological control of white mold on mustard Reviewed

    Md Muzahid E. Rahman, Delwar M. Hossain, Kazuki Suzuki, Ayaka Shiiya, Kazushi Suzuki, Tapan Kumar Dey, Masanori Nonaka, Naoki Harada

    AUSTRALASIAN PLANT PATHOLOGY   45 ( 1 )   103 - 117   2016.2

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    Biological control, especially with Bacillus-based biocontrol agents, offers an attractive alternative to synthetic pesticides for sustainable management of white mold disease caused by Sclerotinia sclerotiorum. In this study, eight effective Bacillus isolates were isolated from rhizospheric soil samples as potential bacterial biocontrol agents. Cultural, biochemical, and molecular analyses of 16S rDNA and gyrase subunit A (gyrA) confirmed that all isolates were identified as Bacillus amyloliquefaciens subsp. plantarum. The production of hydrolytic enzymes and the plant growth-promotional attributes of these isolates confirmed their multifaceted potential. Molecular analysis of the eight biosynthetic genes, which are related to antibiotic properties of bacilli, revealed that all of the isolates possess five genes: bacA for bacilysin, dfnM for difficidin, fenA for fengycin, ituA for iturin, and sfp for surfactin. The Bacillus isolates inhibited mycelial growth and suppressed formation of sclerotia during an in vitro test against S. sclerotiorum. Deformities and cell-wall lysis of mycelia, abnormalities of apothecia, and germination failure of ascospores through interaction with the Bacillus isolates were observed with light and scanning electron microscopes, suggesting that they have high antagonistic effects against S. sclerotiorum. Seed bacterization with the Bacillus isolates protected mustard seedlings in vitro up to 98 % against S. sclerotiorum. In a pot experiment, damages of mustard plants against the pathogen decreased up to 90 % after foliar spray of the Bacillus isolates. In addition, the isolates increased seed germination and accelerated seedling vigor of mustard, suggesting that they have plant growth-promoting functions.

    DOI: 10.1007/s13313-016-0397-4

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  • Arbuscular mycorrhizal fungal community of wheat under long-term mineral and organic amendments in semi-arid Mediterranean Turkey Reviewed

    Takuya Buto, Kazuki Suzuki, Tomoyuki Kaidzu, Taishi Narisawa, Oguz Can Turgay, Ibrahim Ortas, Naoki Harada, Masanori Nonaka

    ARID LAND RESEARCH AND MANAGEMENT   30 ( 4 )   479 - 489   2016

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS INC  

    A minimal amount of information is currently available concerning arbuscular mycorrhizal (AM) fungal associations with crops in semi-arid zones on Leptosols in Turkey. Therefore, using molecular ecological techniques, we studied the effects of different management practices (without fertilization, chemical fertilization, farmyard manure, and plant compost amendments) on AM fungal communities associated with wheat roots. Experiments were conducted in a field established in 1996 in southern Mediterranean Turkey where soil productivity is low owing to unfavorable climatic effects and soil characteristics. We determined 201 partial sequences of AM fungal nuclear ribosomal large subunit genes. The higher AM fungal richness was found in the control treatment without fertilization and plant compost treatments compared with the chemical fertilization and farmyard manure treatments. Clones related to Rhizophagus were found in all treatments and accounted for 37% of the total AM fungal clones, whereas those of Funneliformis were dominant under chemical fertilization. Redundancy analysis based on the frequency of operational taxonomic units revealed that AM fungal communities were divided into three groups, namely, the control treatment, the chemical fertilization treatment, and the organic treatments (farmyard manure and plant compost treatments). Although different organic amendments supported relatively similar AM fungal communities, plant compost induced higher AM fungal richness than farmyard manure fertilization.

    DOI: 10.1080/15324982.2016.1177749

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  • 8-1-28 改植による桑の葉の<sup>137</sup>Cs 濃度の低減(8-1 環境保全,2016年度佐賀大会)

    木幡 裕介, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   62   152 - 152   2016

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    Language:Japanese   Publisher:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.62.0_152_1

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  • 8-1-13 水田の水口付近における土壌及びイネの放射性セシウム濃度の分布(8-1 環境保全,2016年度佐賀大会)

    鈴木 啓真, 中島 浩世, 鶴田 綾介, 吉川 夏樹, 石井 秀樹, 野川 憲夫, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   62   147 - 147   2016

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    DOI: 10.20710/dohikouen.62.0_147_1

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  • First Report of white mold on Jackfruit caused by Sclerotinia sclerotiorum in Bangladesh Reviewed

    Md Muzahid-E- Rahman, Tapan Kumar Dey, Delwar M Hossain, Masanori Nonaka, Naoki Harada

    Australasian Plant Disease Notes   10 ( 10 )   2015.12

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    DOI: 10.1007/s13314-014-0155-9

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  • Radiocesium contamination of soybean (Glycine max) produced with no fertilization in 2014: A case study in Minamisoma City, Fukushima Prefecture, Japan Reviewed

    Naoki Harada, Saki Ito, Naoto Nihei, Masanori Nonaka

    Japanese Journal of Organic Agriculture Science   7 ( 2 )   35 - 41   2015.12

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  • <sup>137</sup>Cs Distribution in Mulberry(Morus alba) after the Fukushima Dai-ichi Nuclear Power Plant Accident and Effect of Spray Application of a Liquid Potassium Fertilizer onto Trunk Surface Reviewed

    HARADA Naoki, MOTOJIMA Sayaka, IGARASHI Kazuki, NONAKA Masanori

    RADIOISOTOPES   64 ( 10 )   613 - 619   2015.10

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    <i>After the Fukushima Dai‐ichi Nuclear Power Plant accident in March 2011, contamination of mulberry-leaf tea with over 100</i>Bq kg<sup>-1</sup> <sup>137</sup>Cs<i> was found. We therefore investigated </i><sup>137</sup>Cs<i> distribution in mulberry trees(Morus alba) and soil in Nihonmatsu City from 2012. As a result, mulberry leaves near the end of branches(0 - 30</i>cm<i>), which are usually used as edible leaves, were shown to contain higher </i><sup>137</sup>Cs<i> concentration than the others. Trunk showed highest </i><sup>137</sup>Cs<i> concentration among the plant parts of mulberry investigated, while root </i><sup>137</sup>Cs<i> concentration was relatively low. Autoradiographic studies for leaf, bark and cross-sectional trunk suggest that radioactive substances were deposited onto bark and leaf radioactive contamination could be due to translocation of </i><sup>137</sup>Cs<i> from trunk. Spray application of 0.5</i>% KH<sub>2</sub>PO<sub>4</sub><i> solution onto trunk surface significantly reduced </i><sup>137</sup>Cs<i> concentration in edible leaves emerged about one month after the application. However, the effect was limited and insufficient to solve the problem of radioactive contamination in edible mulberry leaves.</i>

    DOI: 10.3769/radioisotopes.64.613

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  • FIRST REPORT OF WHITE MOULD CAUSED BY SCLEROTINIA SCLEROTIORUM ON MARIGOLD (TAGETES ERECTA) IN BANGLADESH Reviewed

    M. M. E. Rahman, D. M. Hossain, T. K. Dey, S. R. Sarker, M. Nonaka, N. Harada

    JOURNAL OF PLANT PATHOLOGY   97 ( 2 )   398 - 398   2015.7

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    DOI: 10.4454/JPP.V97I2.046

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  • Sulfate-reducing bacteria mediate thionation of diphenylarsinic acid under anaerobic conditions Reviewed

    Ling Guan, Ayaka Shiiya, Shihoko Hisatomi, Kunihiko Fujii, Masanori Nonaka, Naoki Harada

    BIODEGRADATION   26 ( 1 )   29 - 38   2015.2

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    Diphenylarsinic acid (DPAA) is often found as a toxic intermediate metabolite of diphenylchloroarsine or diphenylcyanoarsine that were produced as chemical warfare agents and were buried in soil after the World Wars. In our previous study Guan et al. (J Hazard Mater 241-242:355-362, 2012), after application of sulfate and carbon sources, anaerobic transformation of DPAA in soil was enhanced with the production of diphenylthioarsinic acid (DPTAA) as a main metabolite. This study aimed to isolate and characterize anaerobic soil microorganisms responsible for the metabolism of DPAA. First, we obtained four microbial consortia capable of transforming DPAA to DPTAA at a high transformation rate of more than 80 % after 4 weeks of incubation. Sequencing for the bacterial 16S rRNA gene clone libraries constructed from the consortia revealed that all the positive consortia contained Desulfotomaculum acetoxidans species. In contrast, the absence of dissimilatory sulfite reductase gene (dsrAB) which is unique to sulfate-reducing bacteria was confirmed in the negative consortia showing no DPAA reduction. Finally, strain DEA14 showing transformation of DPAA to DPTAA was isolated from one of the positive consortia. The isolate was assigned to D. acetoxidans based on the partial 16S rDNA sequence analysis. Thionation of DPAA was also carried out in a pure culture of a known sulfate-reducing bacterial strain, Desulfovibrio aerotolerans JCM 12613(T). These facts indicate that sulfate-reducing bacteria are microorganisms responsible for the transformation of DPAA to DPTAA under anaerobic conditions.

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  • First report of white mould caused by Sclerotinia sclerotiorum on jackfruit Reviewed

    Md Muzahid E Rahman, Tapan Kumar Dey, Delwar M. Hossain, Masanori Nonaka, Naoki Harada

    Australasian Plant Disease Notes   10 ( 1 )   1 - 3   2015

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    Symptoms of white mould were first observed on jackfruit (Artocarpus heterophyllus) in Rangpur, Bangladesh, during February 2012. Fluffy, white mycelia developed on the fruit surface, along with large irregular black sclerotia. Morphological characteristics and the internal transcribed spacer sequences of ribosomal DNA identified the fungus as Sclerotinia sclerotiorum.

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  • Molecular diversity of indigenous arbuscular mycorrhizal fungi in three different agricultural regions of Turkey Reviewed

    Kazuki Suzuki, Oguz Can Turgay, Muhittin Onur Akca, Naoki Harada, Masanori Nonaka

    SOIL SCIENCE AND PLANT NUTRITION   60 ( 3 )   367 - 376   2014.6

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    Little is known about the distribution of arbuscular mycorrhizal fungi (AMF, phylum Glomeromycota) in the Turkish arable soils. In this study, we investigated AM fungal phylotype composition in the roots of 13 different plant samples from one site each of the East Black Sea, Mediterranean, and Central Anatolian regions of Turkey. Fifty-seven distinguished operational taxonomic units at 97% nucleotide sequence identity were recorded among 424 partial sequences of the nuclear ribosomal large subunit (LSU) RNA genes determined. Most of the new sequences were clustered within 10 well-resolved phyloclades of the order Glomerales. About half of the newly determined sequences lacked similar sequences in the public databases. In particular, all sequences from Camellia sinensis collected in the East Black Sea region had only 83-97% sequence similarity to known AMF species. The findings suggest that novel and endemic AMF species may exist in Turkish agricultural soils. The AM fungal community composition in the East Black Sea region was relatively simple and completely differed from those in the other two regions, presumably due to the low soil pH and host specificity. The AM fungal community compositions of the Mediterranean and Central Anatolian samples were broadly similar; however, some sequences related to Rhizophagus were found only in the Mediterranean samples. This reflects the trend that more diverse AM fungal communities are established in the Mediterranean region than the Central Anatolian region.

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  • Cs-137 in irrigation water and its effect on paddy fields in Japan after the Fukushima nuclear accident Reviewed

    Natsuki Yoshikawa, Hitomi Obara, Marie Ogasa, Susumu Miyazu, Naoki Harada, Masanori Nonaka

    SCIENCE OF THE TOTAL ENVIRONMENT   481   252 - 259   2014.5

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    There is concern that radiocesium deposited in the environment after the accident at the Fukushima Daiichi Nuclear Power Plant (FDNPP) in March 2011 will migrate to paddy fields through hydrological pathways and cause serious and long-lasting damage to the agricultural activities. This study was conducted in the Towa region of Nihonmatsu in the northern part of Fukushima Prefecture, Japan, (1) to quantify Cs-137 in stream water used to irrigate paddy fields by separating the dissolved and particulate components in water samples and then fractionating the particulate components bonded in different ways using a sequential extraction procedure, and (2) to determine the amounts of radiocesium newly added to paddy fields in irrigation water relative to the amounts of radiocesium already present in the fields from the deposition of atmospheric fallout immediately after the FDNPP accident. Three catchments were studied, and the Cs-137 activity concentrations in stream water samples were 79-198 mBq L-1 under stable runoff conditions and 702-13,400 Bq L-1 under storm runoff conditions. The residual fraction (F4, considered to be non-bioavailable) was dominant, accounting for 59.5-82.6% of the total 137Cs activity under stable runoff conditions and 69.4-95.1% under storm runoff conditions. The 137Cs newly added to paddy fields in irrigation water only contributed 0.03-0.05% of the amount already present in the soil (201-348 kBq m(-2)). This indicates that the Cs-137 inflow load in irrigation water is negligible compared with that already in the soil. However, the contribution from the potentially bioavailable fractions (F1 + F2 + F3) was one order of magnitude larger, accounting for 0.20-0.59%. The increase in the dissolved and soluble radiocesium fraction (F1) was especially large (3.0% to infinity), suggesting that radiocesium migration in irrigation water is increasing the accumulation of radiocesium in rice. (C) 2014 Elsevier B.V. All rights reserved.

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  • Formation of diphenylthioarsinic acid from diphenylarsinic acid under anaerobic sulfate-reducing soil conditions Reviewed

    Shihoko Hisatomi, Ling Guan, Mami Nakajima, Kunihiko Fujii, Masanori Nonaka, Naoki Harada

    Journal of Hazardous Materials   262   25 - 30   2013.11

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    Diphenylarsinic acid (DPAA) is a toxic phenylarsenical compound often found around sites contaminated with phenylarsenic chemical warfare agents, diphenylcyanoarsine or diphenylchloroarsine, which were buried in soil after the World Wars. This research concerns the elucidation of the chemical structure of an arsenic metabolite transformed from DPAA under anaerobic sulfate-reducing soil conditions. In LC/ICP-MS analysis, the retention time of the metabolite was identical to that of a major phenylarsenical compound synthesized by chemical reaction of DPAA and hydrogen sulfide. Moreover the mass spectra for the two compounds measured using LC/TOF-MS were similar. Subsequent high resolution mass spectral analysis indicated that two major ions at m/z 261 and 279, observed on both mass spectra, were attributable to C12H10AsS and C12H12AsSO, respectively. These findings strongly suggest that the latter ion is the molecular-related ion ([M+H]+) of diphenylthioarsinic acid (DPTA
    (C6H5)2AsS(OH)) and the former ion is its dehydrated fragment. Thus, our results reveal that DPAA can be transformed to DPTA, as a major metabolite, under sulfate-reducing soil conditions. Moreover, formation of diphenyldithioarsinic acid and subsequent dimerization were predicted by the chemical reaction analysis of DPAA with hydrogen sulfide. This is the first report to elucidate the occurrence of DPAA-thionation in an anaerobic soil. © 2013 Elsevier B.V.

    DOI: 10.1016/j.jhazmat.2013.08.020

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  • Effects of diphenylarsinic acid on bacterial and archaeal community structures in an anaerobic paddy soil Reviewed

    Ling Guan, Naoki Harada, Yasushi Ono, Takuya Takahashi, Kunihiko Fujii, Xiaolan Liu, Masanori Nonaka

    GEODERMA   195   243 - 250   2013.3

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    DPAA is often found as one of the major degradation products in soil and groundwater contaminated with diphenylcyanoarsine or diphenylchloroarsine. These compounds were produced mainly during the World Wars as chemical warfare agents. This study investigated the effect of DPAA contamination on soil bacterial and archaeal community structures under anaerobic conditions in a model experiment using 16S rRNA targeted PCR-DGGE fingerprinting of DNA extracted from the contaminated soil. DPAA decreased in anaerobically incubated soil cultures due to microbiological activity. Addition of rice straw partially enhanced the extent of DPAA degradation. Inorganic arsenic acid, PM, and an unknown arsenical species were detected as degradation products using LC-ICPMS. PCR-DGGE fingerprinting for bacterial community analysis revealed that anaerobic bacterial and archaeal species, including Clostridium and Methanosarcina, became abundant in the incubated soil within a week. In the soil cultures incubated with DPAA, while a few bacterial bands found on the DGGE gels disappeared or became weaker, most of the bands showed no significant changes. Addition of DPAA had no significant effect on archaeal communities. These findings suggest that, even though DPAA may have a direct effect on some abundant bacterial species, overall bacterial and archaeal community structures under the anaerobic soil conditions tended to be stable regardless of DPAA contamination. (c) 2012 Elsevier B.V. All rights reserved.

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  • Radioactive Cesium Decontamination and Strategic Farmland Resource Conservation Reviewed

    ARITA Hiroyuki, HASHIMOTO Shizuka, YOSHIKAWA Natsuki, HARADA Naoki, YASUTAKA Tetsuo, NONAKA Masanori, IWASAKI Yumi, MIYAZU Susumu

    Transactions of The Japanese Society of Irrigation, Drainage and Rural Engineering   80 ( 6 )   555 - 561   2012.12

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    Radiocesium (Cs) decontamination of land has been required as one of the restoration measures after the Tohoku-Pacific Ocean Earthquake disaster. The Ministry of Agriculture, Forestry and Fisheries (MAFF) of Japan plans to carry out decontamination measures of farmland in response to the situation of land contamination. However, in order to achieve effective restoration, strategic and comprehensive measures including land readjustment are necessary. In this paper, we examined characteristics of Cs contamination from the view of the spatial extent contamination, land use, land improvement and working environment, and made the following proposals: (1) Implementation of Cs decontamination works ought to follow the principle of &ldquo;containment&rdquo;, &ldquo;isolation&rdquo;, and &ldquo;securing long-term safety&rdquo; of Cs, and improve both production environment and working environment of farmlands. (2) Strategic approaches should be carried out in a comprehensive and unified manner for the effective implementation of Cs decontamination.

    DOI: 10.11408/jsidre.80.555

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  • Enhanced transformation of diphenylarsinic acid in soil under sulfate-reducing conditions Reviewed

    Ling Guan, Shihoko Hisatomi, Kunihiko Fujii, Masanori Nonaka, Naoki Harada

    JOURNAL OF HAZARDOUS MATERIALS   241   355 - 362   2012.11

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    Diphenylarsinic acid (DPAA) is known to be the major contaminant in soils where diphenylchloroarsine and diphenylcyanoarsine were abandoned after World Wars I and II. In this study, experimental model studies were performed to elucidate key factors regulating the transformation of DPAA under anaerobic soil conditions. The elimination of DPAA in Gleysol soils (Qiqihar and Shindori soils) was more rapid than in Mollisol and Regosol soils (Heihe and lkarashi soils, respectively) during a 5-week incubation. No clear relationship between decreasing rates of DPAA concentrations and soil Eh values was found. The Ikarashi soil showed the slowest decrease in DPAA concentrations among the four soils, but the transformation of DPAA was notably enhanced by addition of exogenous sulfate together with acetate, cellulose or rice straw. Addition of molybdate, a specific inhibitor of sulfate reduction, resulted in the stagnation of DPAA transformation, suggesting that indigenous sulfate reducers play a role in DPAA transformation under anaerobic conditions. Arsenate, phenylarsonic acid, phenylmethylarsinic acid, diphenylmethylarsine oxide and three unknown compounds were detected as metabolites of DPAA. This is the first study to reveal enhancement of DPAA transformation under sulfate-reducing conditions. (C) 2012 Elsevier B.V. All rights reserved.

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  • Soil radiocesium distribution in rice fields disturbed by farming process after the Fukushima Dai-ichi Nuclear Power Plant accident Reviewed

    Naoki Harada, Masanori Nonaka

    SCIENCE OF THE TOTAL ENVIRONMENT   438   242 - 247   2012.11

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    A magnitude 9.0 earthquake and subsequent large tsunami hit the northeastern coast of Japan on March 11, 2011. This resulted in serious damage to the reactors of the Fukushima Dai-ichi Nuclear Power Plant (FDNPP), operated by the Tokyo Electric Power Company. Large amounts of radionuclides were released from the FDNPP, a proportion of which were deposited onto the ground. In this study, we investigated soil radiocesium contamination of rice fields in Aga and Minamiuonuma, Niigata, similar to 130 and 200 km away from the FDNPP, respectively, as Niigata is one of the largest rice growing regions in Japan. Soil samples were collected from the plow layer of five rice fields in August and September, 5-6 months after the FDNPP accident. Results showed that radiocesium concentrations (the sum of Cs-134 and Cs-137) in the rice soil samples were similar to 300 Bq (kg dry soil)(-1). All samples contained a Cs-134/Cs-137 activity ratio of 0.68-0.96 after correction to March 11, 2011, showing that the radiocesium released from the FDNPP were deposited on these areas. Although the rice fields had been disturbed by farming processes after the FDNPP accident, the depth distribution of radiocesium concentrations in the plow layers showed higher concentrations in the upper soil layers. This suggests that spring tillage, flooding and puddling performed before rice transplantation may not disperse radiocesium deposited on the surface through the whole plow layer. In addition, the planar distribution of radiocesium concentrations was examined near the water inlet in one of the rice fields. Highest activities were found aligned with the direction of irrigation water discharge, indicating that radioactivity levels in rice fields may be elevated by an influx of additional radionuclides, probably in irrigation water, during farming. (c) 2012 Elsevier B.V. All rights reserved.

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  • Biotransformation of diphenylarsinic acid under anaerobic soil conditions

    Naoki HARADA, Ling GUAN, Shihoko HISATOMI, Mami NAKAJIMA, Kunihiko FUJII, Masanori NONAKA

    Proceedings of SoilRem 2012 SiteRem2012   19 - 21   2012.9

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  • Determination of a sulfur containing arsenic metabolite in the anaerobic soil culture contaminated with diphenylarsinic acid

    Shihoko HISATOMI, Ling GUAN, Kunihiko FUJII, Mami NAKAJIMA, Masanori NONAKA, Naoki HARADA

    Proceedings of SoilRem 2012 SiteRem2012   36 - 38   2012.9

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  • Availability of sulfate and electron donating substrates influences transformation of diphenylarsinic acid in anaerobic soil

    Ling GUAN, Shihoko HISATOMI, Kunihiko FUJII, Masanori NONAKA, Naoki HARADA

    Proceedings of SoilRem 2012 SiteRem2012   29 - 31   2012.9

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  • Biodegradation of melamine and its hydroxy derivatives by a bacterial consortium containing a novel Nocardioides species Reviewed

    Kazuhiro Takagi, Kunihiko Fujii, Ken-ichi Yamazaki, Naoki Harada, Akio Iwasaki

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   94 ( 6 )   1647 - 1656   2012.6

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    Melamine has recently been recognized as a food contaminant with adverse human health effects. Melamine contamination in some crops arises from soil and water pollution from various causes. To remove melamine from the polluted environment, a novel bacterium, Nocardioides sp. strain ATD6, capable of degrading melamine was enriched and isolated from a paddy soil sample. The enrichment culture was performed by the soil-charcoal perfusion method in the presence of triazine-degrading bacteria previously obtained. Strain ATD6 degraded melamine and accumulated cyanuric acid and ammonium, via the intermediates ammeline and ammelide. No gene known to encode for triazine-degrading enzymes was detected in strain ATD6. A mixed culture of strain ATD6 and a simazine-degrading Methyloversatilis sp. strain CDB21 completely degraded melamine, but the degradation rate of cyanuric acid was slow. The degradation of melamine and its catabolites by the mixed culture was greatly enhanced by including Bradyrhizobium japonicum strain CSB1 in the inoculum and adding ethanol to the culture medium. The melamine-degrading consortium consisting of strains ATD6, CDB21, and CSB1 appears to be potentially safer than other known melamine-degrading bacteria for the bioremediation of farmland and other contaminated sites, as no known pathogens were included in the consortium.

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  • アーバスキュラー菌根菌の群集構造に及ぼす高pHの影響(2012年度土壌微生物学会講演要旨)

    成沢 大志, 小林 由季, 原田 直樹, 野中 昌法

    土と微生物   66 ( 2 )   85 - 85   2012

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    DOI: 10.18946/jssm.66.2_85_3

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  • Biodegradation of diphenylarsinic acid to arsenic acid by novel soil bacteria isolated from contaminated soil Reviewed

    Naoki Harada, Kazuhiro Takagi, Koji Baba, Kunihiko Fujii, Akio Iwasaki

    BIODEGRADATION   21 ( 3 )   491 - 499   2010.6

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    Microorganisms capable of degrading diphenylarsinic acid (DPAA) were enriched from contaminated soil using the soil-charcoal perfusion method. Two novel bacterial strains, L2406 and L2413, that can degrade DPAA in a mineral salt medium supplemented with DPAA as the sole carbon source were isolated. Based on comparative morphology, physiology, and comparison of the 16S rRNA gene sequences, both were presumed to be species closely related to Ensifer adhaerens. As the metabolites, phenylarsonic acid (PAA) was determined by liquid chromatography-mass spectrometry analysis as well as three unknown peaks all of whose molecular weights were estimated to be 278. The increase of m/z = 16 from DPAA in the unknowns suggests monohydroxylation of DPAA at the 2-, 3- and 4-positions. The ability of strains L2406 and L2413 to degrade DPAA was suppressed in iron insufficient conditions, e.g. less than 7.2 mu M iron in the culture medium. These facts strongly suggest the following hypothesis: Monooxygenase works at the initial degradation step of DPAA degradation by the isolates; and direct hydrolysis from DPAA to PAA is not likely to occur. In addition, release of arsenic acid from PAA by strain L2406 was confirmed by liquid chromatography-inductively coupled plasma mass spectrometry. From these results, strain L2406 was considered to be capable of degrading DPAA to arsenic acid via PAA when DPAA was supplied as the sole carbon source.

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  • 新潟県の海岸砂丘地植物と共生するアーバスキュラー菌根菌(2010年度大会一般講演要旨)

    海津 朋之, 武藤 拓也, 原田 直樹, 野中 昌法

    土と微生物   64 ( 2 )   151 - 151   2010

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  • 食品残渣の土壌処理に伴う微生物群集構造変化(2010年度大会一般講演要旨)

    飯尾 真也, 原田 直樹, 野中 昌法

    土と微生物   64 ( 2 )   146 - 146   2010

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  • Does inoculation of anoxygenic photosynthetic bacteria contribute to rice production?

    Naoki Harada

    Development of Integrated Pest Management in Asia and Africa, Science and Technics Publishing House   187 - 194   2009.12

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  • Bacterial degradation of persistent agrochmicals and application of charcoal as a tool for in situ bioremediation

    Naoki Harada

    Development of Integrated Pest Management in Asia and Africa, Science and Technics Publishing House   149 - 156   2009.12

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  • Characteristics of nitrogen dynamics in organic glasshouse tomato in Niigata Prefecture Reviewed

    Masanori NONAKA, Yui FUJINO, Naoki HARADA

    Japanese Journal of Organi Agriculture Science   1 ( 1 )   42 - 51   2009.12

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  • Aerobic Mineralization of Hexachlorobenzene by Newly Isolated Pentachloronitrobenzene-Degrading Nocardioides sp Strain PD653 Reviewed

    Kazuhiro Takagi, Akio Iwasaki, Ichiro Kamei, Koji Satsuma, Yuichi Yoshioka, Naoki Harada

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   75 ( 13 )   4452 - 4458   2009.7

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    A novel aerobic pentachloronitrobenzene-degrading bacterium, Nocardioides sp. strain PD653, was isolated from an enrichment culture in a soil-charcoal perfusion system. The bacterium also degraded hexachlorobenzene, a highly recalcitrant environmental pollutant, accompanying the generation of chloride ions. Liberation of (14)CO(2) from [U-ring-(14)C] hexachlorobenzene was detected in a culture of the bacterium and indicates that strain PD653 is able to mineralize hexachlorobenzene under aerobic conditions. The metabolic pathway of hexachlorobenzene is initiated by oxidative dechlorination to produce pentachlorophenol. As further intermediate metabolites, tetrachlorohydroquinone and 2,6-dichlorohydroquinone have been detected. Strain PD653 is the first naturally occurring aerobic bacteria capable of mineralizing hexachlorobenzene.

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  • Different substrate specificities of two triazine hydrolases (TrzNs) from Nocardioides species Reviewed

    Kenichi Yamazaki, Kunihiko Fujii, Akio Iwasaki, Kazuhiro Takagi, Koji Satsuma, Naoki Harada, Tai Uchimura

    FEMS MICROBIOLOGY LETTERS   286 ( 2 )   171 - 177   2008.9

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    Nocardioides sp. strain MTD22 degraded atrazine, ametryn and atraton, as did Arthrobacter aurescens strain TC1 and Nocardioides sp. strain C190. These strains contain trzN, a gene coding for TrzN, triazine hydrolase showing a broad substrate range. However, Nocardioides sp. strain AN3 degraded only atrazine despite containing trzN. These differences in s-triazine degradation are presumed to be due to differences in the amino acid sequences of TrzNs. Consequently, 1371 nucleotides of the trzN coding sequences of strains AN3 and MTD22 were determined. Comparisons of the amino acid sequences of TrzNs indicated that three residues of strain AN3 (Thr(214), His(215) and Gln(241)) were distinct from those of the other three strains (Pro(214), Tyr(215) and Glu(241)). To confirm the relationships between these amino acid sequences and the substrate specificities of TrzNs, wild and chimera trzN genes were constructed and expressed in Escherichia coli cells. Cells expressing wild MTD22 trzN (Pro(214)Tyr(215)Glu(241)) and chimera AN3-MTD22 trzN (Thr(214)His(215)Glu(241)) degraded all s-triazines, but the degradation rate was markedly decreased in AN3-MTD22 trzN. Wild AN3 trzN (Thr(214)His(215)Gln(241)) and chimera MTD22-AN3 trzN (Pro(214)Tyr(215)Gln(241)) degraded only atrazine. These results suggest that the substitution of Glu(241) for Gln(241) significantly decreases enzyme affinity for ametryn and atraton.

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  • Simultaneous biodegradation of chloro and methylthio-s-triazines using charcoal enriched with a newly developed bacterial consortium Reviewed

    Ken-ichi Yamazaki, Kazuhiro Takagi, Kunihiko Fujii, Akio Iwasaki, Naoki Harada, Tai Uchimura

    JOURNAL OF PESTICIDE SCIENCE   33 ( 3 )   266 - 270   2008

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    A special type of charcoal, Charcoal A 100, was enriched with a newly developed bacterial consortium using a perfusion method. The bacterial consortium consisted of a methylthio-s-triazine-degrading bacterium (Rhodococcus sp. FJIII7YT) and the chloro-s-triazine-degrading bacterial consortium CD7 (containing Bradyrhizobium japonicam CSBI, Arthrobacter sp. CD7w and beta-Proteobacteria CDB21). Enriched charcoal was capable of degrading chloro-s-triazines (simazine and atrazine) and methylthio-s-triaiznes (simazine and atrazine) and methylthio-s-triazines (simetryn and dimethametryn) simultaneously in sulfur-free medium. Almost complete degradation was observed after 4-day cultivation of chloro-s-triazines and 9-day cultivation of methylthio-s-triazines. These triazines were mineralized via their 2-hydroxy analogues.

    DOI: 10.1584/jpestics.G08-08

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  • Enhanced methanogenesis in the vicinity of rice straw residues in surface layers of a paddy soil Reviewed

    Harada N, Nishiyama M, Otsuka S, Matsumoto S

    土と微生物   61 ( 1 )   17 - 21   2007.4

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    Methanogenesis in irrigated rice fields is carried out by methanogenic archaea under strictly anaerobic conditions. In the present study, the effect of rice straw on methanogenesis in surface layers of paddy soil was investigated in a pot experiment with surface application of rice straw. Rice straw application led to a significant development of reduced conditions in the surface soil, and markedly increased methane (CH_4) emissions. Potential methanogenic activity in the straw residues and the soil layer immediately under the residues (0〜1 cm depth) was considerably promoted, while that in the deeper soil layers (4〜6 cm depth) was not affected by the residues of rice straw. These results indicate that the increased CH_4 emission in the treatment with surface application of rice straw was due to the activation of methanogenesis in the vicinity of the residues. Consequently, in the present study, it was demonstrated that rice straw application enhanced methanogenesis even in the surface layers of paddy soil, which are generally considered to be oxic layers.

    DOI: 10.18946/jssm.61.1_17

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    Other Link: http://hdl.handle.net/10191/24984

  • Isolation and characterization of a novel simazine-degrading beta-proteobacterium and detection of genes encoding s-triazine-degrading enzymes Reviewed

    Akio Iwasaki, Kazuhiro Takagi, Yuichi Yoshioka, Kunihiko Fujii, Yuki Kojima, Naoki Harada

    PEST MANAGEMENT SCIENCE   63 ( 3 )   261 - 268   2007.3

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    A moderately persistent herbicide, simazine, has been used globally and detected as a contaminant in soil and water. The authors have isolated a simazine-degrading bacterium from a simazine-degrading bacterial consortium that was enriched using charcoal as a microhabitat. The isolate, strain CDB21, was gram-negative, rod-shaped (0.5-0.6 mu m x 1.0-1.2 mu m) and motile by means of a single polar flagellum. Based on 16S rRNA sequence analysis, strain CDB21 was identified as a novel beta-proteobacterium exhibiting 100% sequence identity with the uncultured bacterium HOClCi25 (GenBank accession number AY328574). PCR using primers that were specific for the genes of the atrazine-degrading enzymes (atzABCDEF) of Pseudomonas sp. strain ADP showed that strain CDB21 also possessed the entire set of genes of these enzymes. Nucleotide sequences of the atzCDEF genes of strain CDB21 were 100% identical to those of Pseudomonas sp. strain ADP. Sequence identity of the atzA genes between these bacteria was 99.7%. The 398-nucleotide upstream fragment of the atzB gene of strain CDB21 was 100% identical to ORF30 of Pseudomonas sp. strain ADP, and the 1526-nucleotide downstream fragment showed 99.8% sequence similarity to the atzB gene of the pseudomonad. (c) 2007 Society of Chemical Industry.

    DOI: 10.1002/ps.1334

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  • Biodegradation of methylthio-s-triazinessd where science by Rhodococcus sp strain FJ1117YT, and production of the corresponding methylsulfinyl, methylsulfonyl and hydroxy analogues Reviewed

    Kunihiko Fujii, Kazuhiro Takagi, Syuntaro Hiradate, Akio Iwasaki, Naoki Harada

    PEST MANAGEMENT SCIENCE   63 ( 3 )   254 - 260   2007.3

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    A novel bacterial strain FJ1117YT was isolated from an enrichment culture with the herbicide simetryn. The isolate was capable of degrading the herbicide supplied as the sole sulfur source in an aquatic batch culture. The strain FJ1117YT was identified as that belonging to Rhodococcus sp. on the basis of comparative morphology, physiological characteristics and comparison of the 16S rRNA gene sequence. The biodegradation pathway of simetryn was established by isolating the methylsulfinyl analogue as the first metabolite and by identification of the methylsulfonyl intermediate and the hydroxy analogue by liquid chromatography-mass spectrometry (LC-MS) and/or nuclear magnetic resonance (NMR) analysis. The results indicate that the methylthio group was progressively oxidised and hydrolysed by the strain FJ1117YT. The same strain is also able to metabolise other methylthio-s-triazines such as ametryn, desmetryn, dimethametryn and prometryn through similar pathways. (c) 2007 Society of Chemical Industry.

    DOI: 10.1002/ps.1331

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  • Adsorptive characteristics of phosphorylated cellulose material against cadmium Reviewed

    Harada N, Suda M, Saito Y, Yabusaki K

    Japanese Journal of Soil Science and Plant Nutrition   78 ( 1 )   89 - 91   2007.2

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    DOI: 10.20710/dojo.78.1_89

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    Other Link: http://agriknowledge.affrc.go.jp/RN/2010734296

  • Transformation of methylthio-s-triazines via sulfur oxidation by strain JUN7, a Bacillus cereus species Reviewed

    Naoki Harada, Kazuhiro Takagi, Kunihiko Fujii, Akio Iwasaki

    SOIL BIOLOGY & BIOCHEMISTRY   38 ( 9 )   2952 - 2957   2006.9

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    It is known that methylthio-s-triazines can be transformed to the corresponding 2-hydroxy derivatives through sulfoxides and sulfones in aerobic and flooded soil; however, production of sulfoxides and/or sulfones from methylthio-s-triazines by isolated s-triazine-degrading bacteria has not been reported yet. In the present study, a new bacterial strain, JUN7, was obtained from Japanese soil; the bacterium is capable of transforming simetryn to 2-methylsulfinyl 4,6-bis(ethylamino)-s-triazine (sulfoxide simetryn) and 2-hydroxy-4,6-bis(ethylamino)-s-triazine (2-hydroxy simetryn) in a Luria-Bertani (LB) medium. This is the first isolation of the specific microorganism that mediates sulfur oxidation of methylthio-s-triazines, as far as we know. Strain JUN7 could decrease other methylthio-s-triazines such as dimethametryn and prometryn, but not chlorinated s-triazines (atrazine, simazine, and terbuthylazine) and methoxy-s-triazine (atraton) in 1/10 LB medium. Strain JUN7 did not possessed gene atzA or trzN encoding triazine-degrading enzymes, suggesting that the strain may have another metabolic system. Characterizations of strain JUN7 based on comparative morphology, physiological classification, and comparison of the partial 16S rRNA sequence indicated that it is assigned as a Bacillus cereus species. (c) 2006 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.soilbio.2006.03.018

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  • Isolation and characterization of microorganisms capable of hydrolysing the herbicide mefenacet Reviewed

    N Harada, K Takagi, A Harazono, K Fujii, A Iwasaki

    SOIL BIOLOGY & BIOCHEMISTRY   38 ( 1 )   173 - 179   2006.1

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    Three strains-Nocardioides sp. MFC-A, Rhodococcus rhodochrous MFC-B, and Stenotrophomonas sp. MFC-C-were newly isolated from rice soil or bark compost as microorganisms capable of cometabolically degrading mefenacet (2-benzothiazol-2-yloxy-N-methylacetanilide). Several other species belonging to the genera Nocardioides, Rhodococcus, and Stenotrophomonas were also capable of degrading mefenacet, suggesting that the ability is not specific to these isolates, but common at least in these genera. N-methylaniline and 2-benzothiazoloxy acetic acid were identified as the metabolites by liquid chromatography-mass spectrometry. Besides mefenacet, the isolates could degrade one or more other amide pesticides, such as flutolanil, mepronil, metolachlor, and pretilachlor. These results indicate that the strains Nocardioides sp. MFC-A, Rhodococcus rhodochrous MFC-B, and Stenotrophomonas sp. MFC-C degrade mefenacet via hydrolysis of the amide bond, but their substrate specificities differ. (c) 2005 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.soilbio.2005.04.015

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  • Effects of inoculation of phototrophic purple bacteria on grain yield of rice and nitrogenase activity of paddy soil in a pot experiment Reviewed

    N Harada, M Nishiyama, S Otsuka, S Matsumoto

    SOIL SCIENCE AND PLANT NUTRITION   51 ( 3 )   361 - 367   2005.6

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    Phototrophic purple bacteria (PPB) are one group of dinitrogen (N-2)-fixing bacteria often found in the anoxic and photic zone of paddy soil. In the present study, the effects of inoculation of PPB on grain yield of rice and changes in their populations and nitrogenase activity were investigated with and without surface application of rice straw in a pot experiment. As an inoculant, Rhodopseudomonas palustris strain KN122 isolated from a paddy soil was selected, and the cell suspension was inoculated into the floodwater once or three times during the cultivation. As a result, the inoculation of PPB increased the grain yield of rice. Compared to the control, the grain yield was 9% higher in the inoculated plot without rice straw application. The treatment was more effective in combination with rice straw application. In the plots where PPB were inoculated with rice straw, grain yields were 21% (single inoculation) and 29% (triple inoculation) higher. Populations of PPB in the plots without rice straw application significantly increased by the inoculation, unlike those in the plots with rice straw application, except for the bulk soil. Phototrophic and heterotrophic nitrogenase activities (acetylene-reducing activity) associated with the soils or residues of the rice straw were not affected by PPB inoculation throughout the experiment. This indicates that the inoculation of PPB into floodwater may not be effective for enhancing N2 fixation in paddy soils and that the beneficial effect of PPB inoculation on the grain yield of rice may be due to unidentified functions of PPB other than biological N-2 fixation.

    DOI: 10.1111/j.1747-0765.2005.tb00041.x

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  • Influences of indigenous phototrophs on methane emissions from a straw-amended paddy soil Reviewed

    N Harada, S Otsuka, M Nishiyama, S Matsumoto

    BIOLOGY AND FERTILITY OF SOILS   41 ( 1 )   46 - 51   2005.1

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    A pot experiment was conducted to investigate the influences of indigenous phototrophs on methane (CH4) emissions from a paddy soil where rice straw was incorporated or was surface-applied. During the cultivation, half of the pots were covered with aluminum foil, except for the minimum space for rice plants, to prevent ambient light reaching the floodwater or the soil surface. Growth of oxygen-producing phototrophs was hardly observed in the unilluminated plots, whereas intensive growth of algae, duckweed and hydrophytes was found in the illuminated ones. Plant growth was not affected by the different treatments. Seasonal changes in CH4 emission determined by a closed chamber method indicated that illumination had no or only minor effects on CH4 emissions when rice straw was incorporated or was not applied, but significantly reduced CH4 emissions when rice straw was surface-applied. Methanogenesis occurring in the soil-floodwater interface was further investigated in two lab-scale model experiments measuring methanogenic activity. As a result, more activated methanogenesis was found in the surface-applied rice straw and the soil around the straw compared with the soil incubated without rice straw. The magnitude of the methanogenic activity in the rice straw incubated under illuminated conditions was significantly lower than that incubated in the dark. Consequently, this study demonstrates that methanogenesis in paddy soil occurs even in the soil-floodwater interface if plant residues like rice straw exist, and such methanogenesis is likely to be suppressed by growth of indigenous phototrophs under illumination.

    DOI: 10.1007/s00374-004-0793-8

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  • Monitoring of a simazine-degrading bacterium during in situ bioremediation study by the use of specific DNA probes

    Iwasaki A, Takagi K, Yoshioka Y, Kojima Y, Harada N

    Proceedings of the 2nd International Conference on Soil Pollution and Remediation   Vol.2   235 - 236   2004.11

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  • Isolation and characterization of novel simazine-degrading bacteria

    Iwasaki A, Takagi K, Yoshioka Y, Harada N

    Proceedings of the Third International Conference on Contaminants in the Soil Environment in the Australasia-Pacific Region   Vol.3   43 - 43   2003.11

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  • Characteristics of phototrophic purple bacteria isolated from a Japanese paddy soil Reviewed

    N Harada, S Otsuka, M Nishiyama, S Matsumoto

    SOIL SCIENCE AND PLANT NUTRITION   49 ( 4 )   521 - 526   2003.8

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    Phototrophic purple bacteria (PPB) were isolated from a Japanese paddy soil and their microbiological characteristics were then analyzed based on comparative morphology, chemotaxonomy, and comparison of 16S rDNA sequences. As a result, 18 strains of PPB were isolated, of which 17 strains were identified as Rhodopseudomonas palustris strains. The other was as a Rubrivivax gelatinosus strain. All the isolates showed a positive reaction in the acetylene reduction assay in the N-reduced medium. Effects of inoculation of the isolates into the soil slurries on soil acetylene-reducing activity (ARA) and methane (CH4) emissions from the slurries were examined using washed cells. The inoculated slurries showed a positive ARA reaction after 10-d incubation under illumination, unlike the control. CH4 emissions from the soil slurries 10-d after the inoculation of the isolates identified as Rps. palustris strains were significantly lower (44-62%) than those from the slurries without inoculants. These results suggest that PPB contribute to the enhancement of soil nitrogen fixation and mitigation of CH4, emissions from paddy soils.

    DOI: 10.1080/00380768.2003.10410041

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  • Phototrophic N-2 fixation suppressed by activated sulfate reduction in anoxic rice soil slurries Reviewed

    N Harada, M Nishiyama, S Matsumoto

    CURRENT MICROBIOLOGY   42 ( 6 )   393 - 397   2001.6

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    Interactions between sulfate reduction (SR) and phototrophic nitrogenase activities were investigated in rice soil slurries mixed with rice straw. Activation of SR by adding exogenous sulfate suppressed acetylene-reducing activity (ARA) of the slurries, which was associated with phototrophic purple bacteria (PB) enumerated to 10(8)-10(9) MPN g(-1) dry weight (dw) soil. Adding 5 mM sodium molybdate, an inhibitor of SR, markedly increased ARA. However, in the slurries receiving both molybdate and exogenous sulfate, the effects declined simultaneously with partial recovery of SR. These results indicate outcompetition of sulfate-reducing bacteria (SRB) with PB in rice soil, when sulfate concentrations are high enough to support SR. The increasing effects of molybdate on ARA continued during the incubation in the sulfate-depleted condition, probably because of absence of SR and toxicity of molybdate to methanogenesis. Accordingly, stopping activities of the competitive microorganisms may be efficient to increase N-2 fixation in rice soil.

    DOI: 10.1007/s002840010236

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  • Inhibition of methanogens increases photo-dependent nitrogenase activities in anoxic paddy soil amended with rice straw Reviewed

    N Harada, M Nishiyama, S Matsumoto

    FEMS MICROBIOLOGY ECOLOGY   35 ( 3 )   231 - 238   2001.5

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    The interaction between phototrophic dinitrogen fixers and methanogens was examined in soil slurries amended with rice straw using 2-bromoethanesulfonic acid (BES), a specific methanogenic inhibitor. Slurries incubated in light increased phototrophic nitrogenase activity (acetylene reducing activity), and showed growth of phototrophic purple bacteria and reduction of CH4 emission, indicating outcompetition of purple bacteria with methanogens in photic zones. Adding BES effectively inhibited methane production and markedly increased phototrophic acetylene reducing activity accompanied with acetate accumulation, but did not affect populations of purple bacteria in the slurries. More acetate accumulated in the inhibited slurries incubated in dark. We suggest that increased availability of organic substrates for purple bacteria after slopping methanogenic consumption by BES caused the increased phototrophic acetylene reducing activity. These results indicate that, after purple bacteria glow enough, performance of their N-2 fixation may be limited by substrate availability, which methanogenesis may profoundly influence. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0168-6496(01)00095-2

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  • 土壌微生物学

    原田 直樹( Role: Joint author ,  第13章 微生物による環境汚染物質などの分解)

    2018.8 

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  • 農と土のある暮らしを次世代へ-原発事故からの農村の再生

    原田 直樹( Role: Joint author ,  第1部第2章 農地の放射性セシウム汚染と作物への影響)

    コモンズ  2018.7 

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  • BISHAMONの軌跡-II -福島支援5年間の記録

    原田 直樹( Role: Joint author ,  第4章3. 大豆の放射性セシウム吸収抑制に向けた取組み 及び 第4章4. 地域特産物に目を向けて~桑葉の放射性セシウム対策~)

    2016.11 

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  • 放射線災害後の福島の農業-数値が語るこの10年- Invited

    原田直樹

    耕   150   35 - 40   2021.3

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  • P3-1-3 有機及び慣行水田における土壌微生物群集構造の比較-長野および宮城におけるケーススタディ-(ポスター,3-1 土壌生物の生態と機能,2018年度神奈川大会)

    鈴木 一輝, 今野 凛, 米山 菜穂子, 三木 孝昭, 徐 啓聡, 大久保 慎二, 岩石 真嗣, 原田 直樹

    日本土壌肥料学会講演要旨集   64 ( 0 )   34 - 34   2018

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    Language:Japanese   Publisher:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.64.0_34_3

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  • 住民と大学・研究機関との連携による放射線計測と試験栽培-福島県飯舘村大久保外内地区-

    石井秀樹, 服部正幸, 棚橋知春, 小松知未, 後藤淳, 内藤航, 上坂元紀, 原田直樹, 野中昌法, 守友裕一

    福島大学地域創造   29   46 - 56   2017.9

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  • ロシア沿海地方における大豆栽培

    大山卓爾, 原田直樹, 小野祐樹, 長谷川英雄

    新潟アグロノミー   51 ( 28 )   33   2015.12

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  • 33 太田川流域水田における放射性セシウムの挙動と水稲への吸収(関東支部講演会,2014年度各支部会)

    荘司 亮介, 片桐 優亮, 小笠 真理恵, 田巻 翔平, 奥村 健郎, 石井 秀樹, 野川 憲夫, 吉川 夏樹, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 61 )   264 - 264   2015.9

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  • P8-1-16 桑園における放射性Csの吸収特性と吸収抑制法の検討(ポスター,8-1 環境保全,2015年度京都大会)

    木幡 裕介, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 61 )   170 - 170   2015.9

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  • P8-1-9 化学肥料(硫酸カリ)と有機質肥料によるダイズの放射性セシウム吸収抑制効果の比較(ポスター,8-1 環境保全,2015年度京都大会)

    本島 彩香, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 61 )   168 - 168   2015.9

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  • P8-1-5 水稲栽培期間における水田土壌表層への放射性セシウムの蓄積(ポスター,8-1 環境保全,2015年度京都大会)

    荘司 亮介, 鈴木 啓真, 弦巻 貴大, 田巻 翔平, 吉川 夏樹, 石井 秀樹, 野川 憲夫, 原田 直樹, 野中 昌大

    日本土壌肥料学会講演要旨集   ( 61 )   167 - 167   2015.9

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  • P8-1-4 農業用水がイネの放射性セシウム濃度に与える影響 : 流下試験による検討(ポスター,8-1 環境保全,2015年度京都大会)

    鈴木 啓真, 荘司 亮介, 弦巻 貴大, 吉澤 涼太, 田巻 翔平, 吉川 夏樹, 石井 秀樹, 野川 憲夫, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 61 )   166 - 166   2015.9

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  • 地中海沿岸地域における長期有機連用がトウモロコシ根の糸状菌叢に与える影響(2015年度大会講演要旨)

    鈴木 一輝, 戸井田 千佳, Turgay Oguz Can, Ortas Ibrahim, 原田 直樹, 野中 昌法

    土と微生物   69 ( 2 )   128 - 128   2015

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  • 次世代シーケンサーを用いた土壌細菌群集構造解析による有機水田土壌のプロファイリングの試み(2015年度大会講演要旨)

    竹村 真奈美, 鈴木 一輝, 野中 昌法, 原田 直樹

    土と微生物   69 ( 2 )   125 - 125   2015

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  • 8-1-19 中山間地水田における放射性セシウムの挙動 : 福島第一原発事故後3年間の経年変化(8-1 環境保全,2014年度東京大会)

    原田 直樹, 宮本 昇平, 吉澤 涼太, 小原 ひとみ, 小笠 真理恵, 宮津 進, 吉川 夏樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 60 )   146 - 146   2014.9

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  • 8-1-10 カリ施肥によるダイズの放射性Cs吸収抑制(8-1 環境保全,2014年度東京大会)

    本島 彩香, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 60 )   143 - 143   2014.9

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  • P3-1-16 深層施肥された窒素のアーバスキュラー菌根菌を介した植物への吸収(ポスター,3-1 土壌生物の生態と機能,2014年度東京大会)

    柏 直也, 岩佐 悦道, 佐藤 孝, 大山 卓爾, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 60 )   41 - 41   2014.9

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  • 中国黒竜江省に分布するアルカリ塩類土壌におけるアーバスキュラー菌根菌の多様性(2013年度大会一般講演要旨)

    成沢 大志, 原田 直樹, 野中 昌法, 司 振江, 黄 彦

    土と微生物   68 ( 1 )   44 - 44   2014.4

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  • P8-1-20 土壌特性がマメ科作物の放射性セシウム吸収に与える影響(ポスター,8-1 環境保全,2013年度名古屋大会)

    本島 彩香, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 59 )   173 - 173   2013.9

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  • 50 有機稲作が水田土壌の細菌群集構造に与える影響の解析(関東支部講演会,2012年度各支部会)

    工藤 航, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 59 )   277 - 277   2013.9

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  • P8-1-24 農業用水からの谷地田への放射性セシウム流入と水稲への吸収(ポスター,8-1 環境保全,2013年度名古屋大会)

    吉澤 涼太, 宮本 昇平, 小原 ひとみ, 小笠 真理恵, 宮津 進, 吉川 夏樹, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 59 )   174 - 174   2013.9

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  • 37 石灰窒素深層施肥によるダイズ畑からの亜酸化窒素発生抑制効果の検証(関東支部講演会,2012年度各支部会)

    池澤 弘, 原田 直樹, 南雲 芳文, 服部 誠, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 59 )   273 - 273   2013.9

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  • 7-1-11 窒素深層施肥による水田フル活用 : (7)転換畑における亜酸化窒素の発生抑制効果(7-1 肥料および施肥法,2013年度名古屋大会)

    池澤 弘, 坂入 恵美子, 吉本 悠太郎, 南雲 芳文, 服部 誠, 藤田 与一, 野中 昌法, 原田 直樹

    日本土壌肥料学会講演要旨集   ( 59 )   127 - 127   2013.9

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  • 里山-谷津田から河川に放出される放射性 セシウムの実態解明

    原田直樹, 野中昌法, 吉川夏樹

    河川整備基金助成事業成果報告書   24–1211–013   1 - 23   2013.5

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  • P3-1-15 トルコ黒海沿岸酸性土壌における施肥管理とチャノキ共生アーバスキュラー菌根菌の多様性(ポスター,3-1 土壌生物の生態と機能,2013年度名古屋大会)

    鈴木 一輝, Turgay Oguz Can, Akca Muhittin Onur, Ali Ergul, Ozer Pmar, 原田 直樹, 野中 昌法

    日土肥講要   59 ( 0 )   44 - 44   2013

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  • P22-10 還元的土壌中におけるジフェニルアルシン酸の変換生成物の同定(S22.放射性セシウムの永年性作物への移行,22.環境保全,2012年度鳥取大会)

    久富 志穂子, Guan Ling, 藤井 邦彦, 中島 真美, 野中 昌法, 原田 直樹

    日本土壌肥料学会講演要旨集   ( 58 )   171 - 171   2012.9

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  • トルコ地中海地域アダナの長期有機連用圃場におけるアーバスキュラー菌根菌の多様性(2012年度土壌微生物学会講演要旨)

    武藤 拓也, 鈴木 一輝, 成沢 大志, 海津 朋之, 原田 直樹, 野中 昌法, Turgay Oguz Can, Ortas Ibrahim

    土と微生物   66 ( 2 )   86 - 86   2012

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    DOI: 10.18946/jssm.66.2_86_1

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  • P8-2 母材および管理の異なるトルコ耕地土壌におけるアーバスキュラー菌根菌の多様性(8.共生,2012年度鳥取大会)

    鈴木 一輝, Turgay Oguz Can, 原田 直樹, 野中 昌法

    日土肥講要   58 ( 0 )   49 - 49   2012

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  • 6-19 有機稲作における窒素固定菌群の解析(第2報) : イネ根から抽出したRNAを用いたnifHの多様性解析(6.土壌生物)

    原田 直樹, 菊地 靖子, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 57 )   41 - 41   2011.8

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  • Fundamental studies on behavior of arsenicals in rice soil

    385 - 390   2010.8

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  • P6-4 有機稲作における窒素固定菌群の解析(第1報) : イネ根におけるnifHの多様性(ポスター紹介,6.土壌生物,2009年度京都大会)

    菊地 靖子, 山家 悠平, 飯尾 真也, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 55 )   51 - 51   2009.9

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  • 6-32 冬期湛水水田における土壌及び稲わらの細菌群集構造の解析(6.土壌生物,2009年度京都大会)

    原田 直樹, 高山 大輝, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 55 )   48 - 48   2009.9

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  • P21-2 アーバスキュラー菌根菌と森林土壌埋土種子由来の植物を用いた自然環境の修復(ポスター紹介,21.緑化技術,2009年度京都大会)

    武藤 拓也, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 55 )   164 - 164   2009.9

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  • 16 食品残渣投入後の土壌化学性及び微生物性の変化(関東支部講演会,2008年度各支部会)

    飯尾 真也, 渡辺 聡, 宮内 直美, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 55 )   271 - 271   2009.9

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  • 17 新潟県の海岸砂丘植物のアーバスキュラー菌根菌の解析(関東支部講演会,2008年度各支部会)

    海津 朋之, 坂田 益朗, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集   ( 55 )   271 - 271   2009.9

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  • 水田土壌における光合成細菌の存在と意義

    原田直樹

    新潟アグロノミー   ( 44 )   47 - 56   2008.12

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  • Substrate specificities of two strains of Nocardioides for degradation of s-triazine herbicides : 1. Relationship between nucleotide sequences of triazine hydrolase gene (trzN) and substrate specificities

    YAMAZAKI Kenichi, TAKAGI Kazuhiro, FUJII Kunihiko, IWASAKI Akio, SATSUMA Koji, HARADA Naoki, UCHIMURA Tai

    33   66 - 66   2008.3

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  • 6-21 ジフェニルアルシン酸分解菌Sinorhizobium sp. L2406株による有機ヒ素化合物の無機化(6.土壌生物,2007年度東京大会)

    原田 直樹, 高木 和広, 馬場 浩司

    日本土壌肥料学会講演要旨集   ( 53 )   42 - 42   2007.8

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  • Biodegradation of s-triazine herbicides by soil microorganisms and their application for in situ bioremediation

    Harada N

    新潟大学農学部研究報告   60 ( 1 )   1 - 7   2007.8

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    Pseudomonas sp. strain ADP has been studied most extensively. Dechlorination of the s-triazine ring by the strain is mediated by atrazine chlorohydrolase (AtzA) and the subsequent degradation is associated with AtzBCDEF. A novel simazine-degrading β-proteobacterium strain CDB21 isolated by the author's group also possessed entire-set of genes of these enzymes (atzABCDEF). However, while atzA is located between ORF30 and atzB in strain ADP, ORF30 fused with atzB in strain CDB21. This indicates that localization of atzA in CDB21 differs from that in the pseudomonad, meaning that genetic variations regarding the degradation genes exist among microorganisms capable of degrading chloro-s-triazines. It is known that methylthio-s-triazines can be transformed to the corresponding hydroxy analogues via sulfur oxidation in aerobic and flooded soil. Recently, two types of bacteria that engage the process were isolated. One is Bacillus cereus strain JUN7 that can degrade methylthio-s-triazines to give the methylsulfinyl and hydroxy analogues under nutrient-rich conditions such as those with the Luria-Bertani medium. The other is Rhodococcus sp. strain FJ1117YT that is able to degrade methylthios-triazines supplied as the sole sulfur source. These strains were unable to degrade chloro-s-triazines. No gene encoding AtzA or TrzN (triazine hydrolase) was found in these strains, suggesting that they may have another metabolic system. Using a special charcoal material enriched with a simazine-degrading bacterial consortium involving strain CDB21, in situ bioremediation study was conducted in a golf course, where simazine is routinely applied for preservation of turf. As a result, the material was effective for preventing penetration of simazine into subsoils and aquatic environments nearby for approximately 2 years. This result suggests that use of adequate habitat materials is preferable to maintain population and activity of inoculant bacteria applied to soil environment.

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    Other Link: http://agriknowledge.affrc.go.jp/RN/2010742733

  • Simultaneous biodegradation of chloro and methylthio s-triazines by a newly developed bacterial consortium (2)

    YAMAZAKI Kenichi, TAKAGI Kazuhiro, HARADA Naoki, FUJII Kunihiko, UCHIMURA Tai

    32   55 - 55   2007.3

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  • Simultaneous biodegradation of chloro and methylthio s-triazines by a newly developed bacterial consortium

    YAMAZAKI Kenichi, TAKAGI Kazuhiro, HARADA Naoki, FUJII Kunihiko, UCHIMURA Tai

    31   42 - 42   2006.3

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  • 6-23 シメトリン分解菌の単離とその性質(6.土壌生物,日本土壌肥料学会 2005年度大会講演要旨集)

    原田 直樹, 高木 和広, 藤井 邦彦, 岩崎 昭夫

    日本土壌肥料学会講演要旨集   ( 51 )   43 - 43   2005.9

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  • In situ bioremediation of pesticide-contaminated soil by using charcoal enriched with consortia of degrading bacteria

    Takagi K, Yoshioka Y, Iwasaki A, Harada N

    Journal of Resources and Environment   41 ( 6 )   79 - 85   2005.5

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  • Characteristics of mefenacet degrading bacteria isolated from Japanese paddy soil and bark compost

    HARADA Naoki, TAKAGI Kazuhiro, HARAZONO Aiko, IWASAKI Akio

    29   110 - 110   2004.3

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  • 6-4 光合成細菌の接種が水田土壌からのメタン発生に与える影響(6.土壌生物)

    原田 直樹, 西山 雅也, 大塚 重人, 松本 聰

    日本土壌肥料学会講演要旨集   ( 48 )   26 - 26   2002.3

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  • 6-19 稲わら施用湛水土壌における光合成細菌に関する研究(第2報) : 光合成細菌の単離とその性質(6.土壌生物)

    原田 直樹, 西山 雅也, 松本 聰

    日本土壌肥料学会講演要旨集   ( 46 )   42 - 42   2000.3

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  • 6-19 稲わら施用湛水土壌における光合成細菌に関する研究(第1報) : メタン生成阻害下でのアセチレン還元活性(ARA)の増大(6.土壌生物)

    原田 直樹, 西山 雅也, 松本 聰

    日本土壌肥料学会講演要旨集   ( 45 )   56 - 56   1999.7

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Presentations

  • Arbuscular Mycorrhizal Fungal diversity in saline-alkaline soils of Turkey International conference

    Tomoyuki KAIDZU, Oguz Can TURGAY, Naoki HARADA, Masanori NONAKA

    The 7th International Conference on Mycorrhiza  2013.1 

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  • Weed control in organic rice farming: effect of surface application of organic materials International conference

    Yamaga Y, Harada N, Nonaka M

    The 5th international meeting for the development of integrated pest management (IPM) in Asia and Africa  2012.12 

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  • 原発事故から1年10ヵ月、ゆうきの里東和ふるさとづくり協議会との協働の復興・研究活動を振り返る・問われる科学者の姿勢!

    野中昌法, 原田直樹, 小松崎将一, 金子信博, 木村園子ドロテア

    第13回日本有機農業学会大会  2012.12 

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  • 有機稲作が水田土壌の細菌群集構造に与える影響の解析

    工藤航, 野中昌法, 原田直樹

    2012年度日本土壌肥料学会関東支部大会  2012.12 

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  • 石灰窒素深層施肥によるダイズ畑からの亜酸化窒素発生抑制効果の検証

    池澤弘, 野中昌法, 原田直樹

    2012年度日本土壌肥料学会関東支部大会  2012.12 

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  • Availability of sulfate and electron donating substrates influences transformation of diphenylarsinic acid in anaerobic soil International conference

    Ling GUAN, Shihoko HISATOMI, Kunihiko FUJII, Masanori NONAKA, Naoki HARADA

    SoilRem2012 & SiteRem2012  2012.9 

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  • Biotransformation of diphenylarsinic acid under anaerobic soil conditions International conference

    Naoki HARADA, Ling GUAN, Shihoko HISATOMI, Mami NAKAJIMA, Kunihiko FUJII, Masanori NONAKA

    SoilRem2012 & SiteRem2012  2012.9 

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  • Determination of a sulfur containing arsenic metabolite in the anaerobic soil culture contaminated with diphenylarsinic acid International conference

    Shihoko HISATOMI, Ling GUAN, Kunihiko FUJII, Mami NAKAJIMA, Masanori NONAKA, Naoki HARADA

    SoilRem2012 & SiteRem2012  2012.9 

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  • 母材および管理の異なるトルコ耕地土壌中の土着アーバスキュラー菌根菌の多様性

    鈴木一輝, 原田直樹, 野中昌法, Oguz Can Turgay

    2012年度日本土壌肥料学会島根大会  2012.9 

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  • 還元的土壌中におけるジフェニルアルシン酸の変換生成物の同定

    久富志穂子, Guan Ling, 藤井邦彦, 中島真美, 野中昌法, 原田直樹

    2012年度日本土壌肥料学会島根大会  2012.9 

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  • Screening of dieldrin-degrading fungi from dieldrin-residual and healthy upland soils International conference

    Emiko SAKAIRI, Masanori NONAKA, Naoki HARADA

    The 4th International Symposium on “Modern Agriculture and Rural Economic Development in Asia”  2012.7 

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  • Diversity of indigenous arbuscular mycorrizal fungi in saline-alkaline soil on Heilongjiang, China International conference

    Taishi NARISAWA, Naoki HARADA, Masanori NONAKA, Zhenjiang SI, Yan HUANG

    The 4th International Symposium on “Modern Agriculture and Rural Economic Development in Asia”  2012.7 

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  • 福島第一原発事故後の新潟県内水田における土壌中放射性セシウムの垂直及び水平分布

    原田直樹, 野中昌法

    第49回 アイソトープ・放射線 研究発表会  2012.7 

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  • 福島第一原発事故による里山-農地生態系の放射性セシウム汚染 ~福島県二本松市における水田土壌及び水稲の放射能濃度~

    宮本昇平, 原田直樹, 野中昌法

    第49回 アイソトープ・放射線 研究発表会  2012.7 

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  • トルコ地中海地域アダナの長期有機連用圃場におけるアーバスキュラー菌根菌の多様性

    武藤拓也, 鈴木一輝, 成沢大志, 海津朋之, 原田直樹, 野中昌法, Oguz Can Turgay, Ibrahim Ortas

    日本土壌微生物学会2012年度大会  2012.6 

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  • アーバスキュラー菌根菌に及ぼす高pHの影響

    成沢大志, 小林由季, 原田直樹, 野中昌法

    日本土壌微生物学会2012年度大会  2012.6 

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  • Arbuscular Mycorrhizal fungal diversity under long-term inorganic and organic fertilization practices in Mediterranean Turkey International conference

    Tomoyuki KAIDZU, Oguz Can TURGAY, Kazuki SUZUKI, Taishi NARISAWA, Takuya BUTO, Naoki HARADA, Masanori NONAKA, Ibrahim ORTAS

    8th International Soil Science Congress, “Land Degradation and Challenges in Soil Management”  2012.5 

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  • 福島および茨城における耕起・不耕起による放射能汚染の土層分布の変化

    小松崎将一, 野中昌法, 原田直樹, 東達哉

    日本農作業学会第47回講演会  2012.3 

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  • 二本松東和地域の里山・水田の放射能汚染の実態と取り組み

    野中昌法, 原田直樹, 小松崎将一

    第12回日本有機農業学会大会  2011.12 

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  • Degradation of diphenylarsinic acid in anaerobic paddy soil and its effects on soil microbial communities International conference

    Ling Guan, Yasushi Ono, Takuya Takahashi, Naoki Harada, Kunihiko Fujii, Xiaolan Liu, Masanori Nonaka

    International Symposium on Environmental Biogeochemistry XX  2011.9 

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  • Expression of nifH genes in roots of rice, Oryza sativa L., under conventional and organic managements International conference

    Naoki Harada, Yasuko Kikuchi, Masanori Nonaka

    International Symposium on Environmental Biogeochemistry XX  2011.9 

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  • Arbuscular mycorrhizal fungi colonizing with coastal plants in Niigata, Japan International conference

    Tomoyuki KAIDZU, Masuro SAKATA, Takuya BUTO, Naoki HARADA, Masanori NONAKA

    International Symposium on Environmental Biogeochemistry XX  2011.9 

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  • 有機稲作における窒素固定菌群の解析(第2報) イネ根から抽出したRNAを用いたnifHの多様性解析

    原田直樹, 菊地靖子, 野中昌法

    日本土壌肥料学会大会  2011.8 

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  • Analysis of soil microbes engaging in anaerobic transformation of diphenylarsinic acid

    Ling GUAN, Naoki HARADA, Masanori NONAKA

    第16回ヒ素シンポジウム  2011.2 

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  • Effects of intentional winter fallow flooding on bacterial community structures of rice soil in Niigata Plain, Japan International conference

    Naoki HARADA, Daiki TAKAYAMA, Masanori NONAKA

    The 4th international meeting for the development of integrated pest management (IPM) in Asia and Africa  2011.1 

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  • Impacts of diphenylarsinic acid on microbial diversities in paddy soil amended with rice straw International conference

    Ling GUAN, Naoki HARADA, Masanori NONAKA

    The 4th international meeting for the development of integrated pest management (IPM) in Asia and Africa  2011.1 

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  • Inoculation effects of arbuscular mycorrhizal fungi on regeneration of shallow landslide scar with seed bank soil in Niigata-Japan forest area International conference

    Takuya BUTO, Naoki HARADA, Masanori NONAKA

    1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation  2010.9 

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  • 食品残渣の土壌処理に伴う微生物群集構造変化

    飯尾真也, 原田直樹, 野中昌法

    日本土壌微生物学会  2010.6 

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  • 新潟県の海岸砂丘地植物と共生するアーバスキュラー菌根菌

    海津朋之, 武藤拓也, 原田直樹, 野中昌法

    日本土壌微生物学会  2010.6 

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  • Diversities of diazotrophic communities in rice soil revealed by molecular-based ecological approaches International conference

    Naoki HARADA, Yasuko KIKUCHI, Masanori NONAKA

    The 3th international meeting for the development of integrated pest management (IPM) in Asia and Africa  2009.12 

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  • Regeneration of shallow landslide slope by seed bank soil and indigenous arbuscular mycorrhizal(AM) fungi in Niigata forest area International conference

    Takuya BUTO, Naoki HARADA, Masanori NONAKA

    The 3rd International Symposium on Strategies for Sustainability in Food Production, Agriculture and the Environment in Asia  2009.9 

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  • Changes in chemical properties and bacterial communities in soil amended with food waste International conference

    Shinya IIO, Naoki HARADA, Masanori NONAKA

    The 3rd International Symposium on Strategies for Sustainability in Food Production, Agriculture and the Environment in Asia  2009.9 

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  • Comparative nitrogen dynamics in organic and conventional tomato cultivation in Niigata prefecture International conference

    Yui FUJINO, Naoki HARADA, Masanori NONAKA

    The 3rd International Symposium on Strategies for Sustainability in Food Production, Agriculture and the Environment in Asia  2009.9 

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  • Arbuscular mycorrhizal fungi (AMF) colonizing with coastal pants in Niigata International conference

    Tomoyuki KAIDZU, Naoki HARADA, Masanori NONAKA

    The 3rd International Symposium on Strategies for Sustainability in Food Production, Agriculture and the Environment in Asia  2009.9 

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  • アーバスキュラー菌根菌と森林土壌埋土種子由来の植物を用いた自然環境の修復

    武藤拓也, 原田直樹, 野中昌法

    日本土壌肥料学会大会  2009.9 

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  • 有機稲作における窒素固定菌群の解析(第1報) イネ根におけるnifHの多様性

    菊地靖子, 山家悠平, 飯尾真也, 原田直樹, 野中昌法

    日本土壌肥料学会大会  2009.9 

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  • 冬期湛水水田における土壌及び稲わらの細菌群集構造の解析

    原田直樹, 高山大輝, 野中昌法

    日本土壌肥料学会大会  2009.9 

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  • Bacterial degradation of persistent agrochemicals and application of charcoal as a tool for in situ bioremediation International conference

    Naoki HARADA

    The 2nd international meeting for the development of integrated pest management (IPM) in Asia and Africa  2008.12 

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  • Does inoculation of anoxygenic photosynthetic bacteria contribute to rice production? International conference

    Naoki HARADA

    The 2nd international meeting for the development of integrated pest management (IPM) in Asia and Africa  2008.12 

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  • 食品残渣投入後の土壌化学性及び微生物性の変化

    飯尾真也, 渡辺 聡, 宮内直美, 原田直樹, 野中昌法

    日本土壌肥料学会関東支部大会  2008.11 

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  • 新潟県の海岸砂丘植物のアーバスキュラー菌根菌の解析

    海津朋之, 坂田益朗, 原田直樹, 野中昌法

    日本土壌肥料学会関東支部大会  2008.11 

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  • Relationship between nucleotide sequences of triazine hydrolase genes and substrate specificities of s-triazines from two Nocardioides species International conference

    Kenichi Yamazaki, Kazuhiro Takagi, Akio Iwasaki, Kunihiko Fujii, Koji Satsuma, Naoki Harada, Tai Uchimura

    1st International Symposium on Water in Agriculture and Forestry: Challenges, Technological Solutions and Innovations  2008.10 

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  • Simultaneous biodegradation of chloro- and methylthio-s-triazines using charcoal enriched with a newly developed bacterial consortium International conference

    Kazuhiro Takagi, Kenichi Yamazaki, Kunihiko Fujii, Akio Iwasaki, Naoki Harada

    The 3rd International Conference on Soil Pollution and Remediation  2008.10 

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  • Different substrate specificities between two strains of Nocardioides in degradation of s-triazines International conference

    Kenichi Yamazaki, Kazuhiro Takagi, Akio Iwasaki, Kunihiko Fujii, Koji Satsuma, Naoki Harada, Tai Uchimura

    The 3rd International Conference on Soil Pollution and Remediation  2008.10 

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  • Nocardioides属細菌2菌株のトリアジン系除草剤に対する基質分解特異性 第1報 Triazine hydrolase 遺伝子(trzN)の塩基配列と基質特異性の関連性

    山崎健一, 高木和広, 藤井邦彦, 岩崎昭夫, 薩摩孝次, 原田直樹, 内村泰

    日本農薬学会第33回大会  2008.3 

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  • Nocardioides属細菌2菌株のトリアジン系除草剤に対する基質分解特異性 第2報 大腸菌で発現された野生型及びキメラtrzNの基質特異性

    藤井邦彦, 高木和広, 薩摩孝次, 山崎健一, 原田直樹, 岩崎昭夫

    日本農薬学会第33回大会  2008.3 

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  • Metabolic pathways of hexachlorobenzene (HCB), quintozene (PCNB) and pentachlorophenol (PCP) by a newly isolated strain Nocardiodes sp. PD653 under aerobic conditions International conference

    Kazuhiro TAKAGI, Yuichi YOSHIOKA, Akio IWASAKI, Ichiro KAMEI, Naoki HARADA

    Dioxin 2007  2007.9 

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  • ジフェニルアルシン酸分解菌Shinorhizobium sp. L2406株による有機ヒ素化合物の無機化

    原田直樹, 高木和広, 馬場浩司

    日本土壌肥料学会大会  2007.8 

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  • 水田土壌からのジフェニルアルシン酸分解菌単離の試み

    原田直樹, 高木和広

    日本土壌肥料学会大会  2006.9 

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  • Aerobic mineralization of hexachlorobenzene by Nocardioides sp. PD653 International conference

    Takagi K, Iwasaki A, Satsuma K, Masuda M, Harada N

    The 11th IUPAC International Congress of Pesticide Chemistry  2006.8 

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  • Bacterial degradation of simetryn, a moderately persistent methylthio-substituted s-triazine herbicide International conference

    Naoki HARADA, Kazuhiro TAKAGI, Kunihiko FUJII, Akio IWASAKI

    International Chemical Congress of Pacific Basin Societies  2005.12 

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  • シメトリン分解菌の単離とその性質

    原田直樹, 高木和広, 藤井邦彦, 岩崎昭夫

    日本土壌肥料学会大会  2005.9 

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  • Monitoring of a simazine-degrading bacterium during in situ bioremediation study by the use of specific DNA probes International conference

    wasaki A, Takagi K, Yoshioka Y, Kojima Y, Harada N

    The 2nd International Conference on Soil Pollution and Remediation  2004.11 

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  • リン酸キレート繊維によるカドミウム汚染水田土壌の浄化1:-リン酸キレート繊維の特徴-

    原田直樹, 須田誠, 斎藤陽子, 薮崎克己

    日本土壌肥料学会大会  2004.9 

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  • リン酸キレート繊維によるカドミウム汚染水田土壌の浄化2:-カドミウム除去能の評価-

    原田直樹, 須田誠, 斎藤陽子, 薮崎克己

    日本土壌肥料学会大会  2004.9 

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  • リン酸キレート繊維による重金属汚染土壌の浄化に関する基礎検討

    原田直樹, 須田誠, 斎藤陽子, 薮崎克己

    第10回地下水・土壌汚染とその防止対策に関する研究集会  2004.7 

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  • 土壌等から単離したmefenacet分解菌の特徴

    原田直樹, 高木和広, 原園愛子, 岩崎昭夫

    日本農薬学会第29回大会  2004.3 

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  • Isolation and characterization of novel simazine-degrading bacteria International conference

    Akio IWASAKI, Kazuhiro TAKAGI, Yuichi YOSHIOKA, Naoki HARADA

    The Third International Conference on Contaminants in the Soil Environment in the Australasia-Pacific Region  2003.11 

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  • 光合成細菌の接種が水田土壌からのメタン発生に与える影響

    原田直樹, 西山雅也, 大塚重人, 松本聰

    日本土壌肥料学会大会  2002.4 

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Research Projects

  • Behavior of radiocesium derived from irrigation water in paddy fields and its effects on radioactivity in rice

    Grant number:19H03072  2019.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

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    Grant amount:\17030000 ( Direct Cost: \13100000 、 Indirect Cost:\3930000 )

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  • ネオニコチノイド系農薬により引き起こされる生態系影響および毒性の実態に迫る

    Grant number:18H04137  2018.4 - 2022.3

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    関島 恒夫, 鈴木 一輝, 原田 直樹, 大西 浩史, 関島 香代子, 吉川 夏樹

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    Grant amount:\44070000 ( Direct Cost: \33900000 、 Indirect Cost:\10170000 )

    以下に、サブテーマごとの研究実績の概要を紹介する。
    S1;クロチアニジンなどネオニコチノイド殺虫剤を水田メソコスムに曝露し、殺虫剤が食物網の形成プロセスに与える影響を解析した。
    S2;水田に施用されたネオニコチノイド系殺虫剤の環境水中の挙動を明らかにし、流域内における汚染リスクを評価するため、(1)調査流域を設定し、(2)殺虫剤の施用状況を流域内のすべての水田で把握し、(3)主要排水路において採水および濃度測定をするとともに、(4)排水路を模した実験水路において、殺虫剤成分の挙動と流出特性に関する実験を行った。
    S3;小ポットを用いて水稲栽培実験を行い、各種ネオニコチノイド系農薬の土壌施用後7週目以降に土壌原生生物数・細菌叢が影響を受けた可能性を見出した。また、土壌中のこれらの農薬の定量法を確立した。今後、各種ネオニコチノイド系農薬が土壌微生物叢に与える影響をより直接的に検討するとともに、確立した定量法を用いてこれら農薬の湛水土壌における光分解や微生物分解、水稲への移行プロセスを追跡する。
    S4;ネオニコチノイド系殺虫剤のヒトへの健康影響が重大と考えられる胎児期・乳児期の汚染状態を捉える上で、人を対象とした研究に向けて、所属施設の「人を対象とした倫理審査」の承認を得る必要がある。受審に向けて、研究チームとして適切な方法を立案すべく、最新のエビデンスを把握し、研究メンバーと検討を進めた。
    S5;予備実験として、妊娠マウスにネオニコチノイド系殺虫剤のクロチアニジンを投与し、産仔数、生育への検討を行った。妊娠2日目-出生後2週間まで投与したが、産仔数や仔の体重に関して薬剤暴露の顕著な影響は認められず、表面上は正常な生育がみられたが、高濃度暴露群で流産が一例認められた。流産の原因は明らかではないが、農薬暴露の影響について胎仔期と生後生育期授乳期を区別した解析の必要性が考えられた。

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  • Why is the concentration of radiocesium in rice near water inlet high?

    Grant number:26511007  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Yoshikawa Natsuki

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    Grant amount:\5070000 ( Direct Cost: \3900000 、 Indirect Cost:\1170000 )

    This study aimed at investigating the phenomenon of higher transfer factor of radiocesium from soil to rice plants near water inlets by quantifying spatial distribution of radiocesium in paddy field water, soil and rice plants at 13 experimental paddy fields in Hama-Dori district in Fukushima prefecture, and developing a numerical simulation model. In consequence, it was suggested that the cause of specifically high concentration of radiocesium in rice near the water inlet may be due to the suspended form of radiocesium accumulated near the water inlet but to the dissolved form. Based on this result,densely transplanting near the water inlet to control the diffusion of suspended solid was attempted as a countermeasure for producing low contamination rice. However,this proposal was found to be failure.
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  • Environmental fate of thionated phenylarsenical compounds

    Grant number:25340084  2013.4 - 2016.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    HARADA NAOKI

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    Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

    In this study, we isolated Desulfotomaculum acetoxidans strain DEA14, which could anaerobically transform diphenylarsinic acid (DPAA) to diphenylthioarsinic acid (DPTA). A known sulfate-reducing bacterial strain, Desulfovibrio aerotolerans JCM 12613T could also transform DPAA to DPTA. The findings suggest that H2S produced by sulfate-reducing bacteria has a role in thionation of DPAA under anaerobic soil conditions. In addition, we confirmed that other phenylarsenicals, such as diphenylmethylarsine oxide and phenylarsonic acid, could be thionated by reaction with H2S. Uptake of phenylarsenicals in rice was also investigated under flooded conditions. At the harvest, DPTA was found in the soil and root samples. In the leaf, stem and brown rice samples, As(V), DPMAO, PMAA and/or DPAA were detected. The results indicate that arsenical species found in rice vary at different parts and the aerial parts of rice contain no DPTA.

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  • バイオレメディエーションを目指した有機ヒ素化合物の嫌気的微生物変換機構の解明

    2010.4 - 2013.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Competitive

    Grant amount:\3000000 ( Direct Cost: \2100000 、 Indirect Cost:\900000 )

    有機ヒ素化合物による土壌汚染は世界各地で大きな問題となっている。その環境動態には微生物の関与が示唆されているが、研究例は非常に少なく未知の点が多い。これまでに我々は、土壌の有機ヒ素汚染物質として最も重要なdiphenylarsinic acid (DPAA)を対象に研究を行い、その好気的分解菌を単離するとともに、さらに還元状態においてはより迅速に微生物変換できることを見出した。そこで本研究では有機ヒ素化合物の還元的微生物変換機構の解明を目的に種々の検討を行う。還元的微生物変換によって毒性が低く土壌吸着しにくい形態のヒ素化合物に変化させることが可能であれば、有機ヒ素汚染土壌に対する新たな修復技術の開発に道を拓くものと期待される。

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  • 稲わら施用量減量による地力低下の程度の確認と水田の冬期湛水化による地力維持の可能性の検討

    2008.8 - 2009.3

    新潟市  受託研究(一般受託研究) 

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    Grant type:Competitive

    Grant amount:\500000

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  • 植物由来有機物を利用した環境修復の研究

    2008.5 - 2009.3

    興和株式会社 興和総合科学研究所  共同研究 

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    Grant type:Competitive

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  • アカミミズの飼育法に関する基礎研究

    2007.11 - 2009.3

    株式会社ミヤトウ野草研究所  共同研究 

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    Grant type:Competitive

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  • 微生物・植物由来バイオマテリアルを用いた環境浄化・改善の研究

    2007.6 - 2008.3

    興和株式会社 興和総合科学研究所  共同研究 

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    Grant type:Competitive

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Teaching Experience (researchmap)

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Teaching Experience

  • スタディ・スキルズAIIc

    2022
    Institution name:新潟大学

  • 統合化学入門

    2022
    Institution name:新潟大学

  • 環境土壌学

    2022
    Institution name:新潟大学

  • スタディ・スキルズAIIb

    2022
    Institution name:新潟大学

  • スタディ・スキルズAIIa

    2022
    Institution name:新潟大学

  • スタディ・スキルズAIa

    2021
    Institution name:新潟大学

  • グローバル農力

    2021
    Institution name:新潟大学

  • スタディ・スキルズAIc

    2021
    Institution name:新潟大学

  • スタディ・スキルズAIb

    2021
    Institution name:新潟大学

  • 農学入門II

    2021
    Institution name:新潟大学

  • 農学入門I

    2021
    Institution name:新潟大学

  • グローバル防災・復興学

    2021
    Institution name:新潟大学

  • 応用生命・食品科学概論

    2021
    Institution name:新潟大学

  • 基礎地学

    2021
    Institution name:新潟大学

  • Topics in Applied Bioresource Chemistry

    2021
    Institution name:新潟大学

  • スタディ・スキルズA b

    2020
    Institution name:新潟大学

  • 資源利用科学特論

    2020
    Institution name:新潟大学

  • 環境土壌学特論

    2019
    -
    2021
    Institution name:新潟大学

  • 農学入門Ⅱ

    2019
    Institution name:新潟大学

  • 農学入門Ⅰ

    2019
    Institution name:新潟大学

  • 土壌学概論

    2018
    Institution name:新潟大学

  • 分析化学(農)

    2018
    Institution name:新潟大学

  • 分析化学実験(農)

    2018
    Institution name:新潟大学

  • 生態系を知る

    2018
    Institution name:新潟大学

  • 環境土壌学

    2018
    -
    2020
    Institution name:新潟大学

  • 環境汚染物質化学

    2018
    Institution name:新潟大学

  • グローバル農力国際特別研究(中期)

    2018
    Institution name:新潟大学

  • グローバル農力国際インターンシップ(長期)

    2018
    Institution name:新潟大学

  • グローバル農力国際特別研究(長期)

    2018
    Institution name:新潟大学

  • 土と水

    2017
    Institution name:新潟大学

  • Topics in Applied Bioresource Chemistry

    2017
    -
    2021
    Institution name:新潟大学

  • 基礎地学

    2017
    -
    2021
    Institution name:新潟大学

  • グローバル農力(短期)

    2017
    -
    2018
    Institution name:新潟大学

  • 応用生物化学セミナーⅡ

    2017
    Institution name:新潟大学

  • 土壌環境学

    2017
    Institution name:新潟大学

  • 応用生命・食品科学セミナーⅡ

    2017
    Institution name:新潟大学

  • グローバル農力国際インターンシップ(中期)

    2016
    -
    2018
    Institution name:新潟大学

  • 応用生命・食品科学概論

    2015
    -
    2021
    Institution name:新潟大学

  • 応用生命・食品科学特論

    2015
    -
    2018
    Institution name:新潟大学

  • 土壌植物資源科学演習 Ⅰ

    2012
    -
    2016
    Institution name:新潟大学

  • 研究発表演習(中間発表)

    2012
    -
    2015
    Institution name:新潟大学

  • 文献詳読Ⅰ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学特定研究BⅡ

    2012
    -
    2015
    Institution name:新潟大学

  • 文献詳読Ⅱ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学特定研究BⅠ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学セミナーBⅡ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学セミナーBⅠ

    2012
    -
    2015
    Institution name:新潟大学

  • 応用生命・食品科学演習(学会発表)

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学セミナーAⅡ

    2012
    Institution name:新潟大学

  • 生命・食料科学特定研究AⅡ

    2012
    Institution name:新潟大学

  • 現代日本事情

    2012
    Institution name:新潟大学

  • リサーチキャンプ

    2012
    Institution name:新潟大学

  • 自然科学総論Ⅳ

    2012
    Institution name:新潟大学

  • 現代日本論

    2012
    Institution name:新潟大学

  • 土壌植物資源科学演習Ⅰ

    2011
    -
    2019
    Institution name:新潟大学

  • 土壌植物資源科学演習Ⅱ

    2011
    -
    2018
    Institution name:新潟大学

  • 土壌化学

    2011
    -
    2013
    Institution name:新潟大学

  • 土壌植物資源科学実験

    2010
    -
    2018
    Institution name:新潟大学

  • 土壌機能解析学特論

    2010
    -
    2017
    Institution name:新潟大学

  • 応用生物化学科インターンシップ

    2010
    -
    2016
    Institution name:新潟大学

  • 科学英語演習

    2010
    -
    2016
    Institution name:新潟大学

  • 応用生物化学概論

    2010
    -
    2016
    Institution name:新潟大学

  • 地学

    2009
    -
    2015
    Institution name:新潟大学

  • スタディ・スキルズA2

    2009
    Institution name:新潟大学

  • 土壌生化学

    2007
    Institution name:新潟大学

  • 分析化学

    2007
    -
    2017
    Institution name:新潟大学

  • 化学実験

    2007
    -
    2017
    Institution name:新潟大学

  • 土壌微生物学特論

    2007
    -
    2016
    Institution name:新潟大学

  • 土壌化学特論

    2007
    -
    2009
    Institution name:新潟大学

▶ display all