2021/10/25 更新

写真a

イズミ ケンジ
泉 健次
IZUMI Kenji
所属
教育研究院 医歯学系 歯学系列 教授
医歯学総合研究科 口腔生命科学専攻 摂食環境制御学 教授
職名
教授
連絡先
メールアドレス
外部リンク

学位

  • 博士(歯学) ( 1992年3月   新潟大学 )

研究キーワード

  • 組織工学

  • Oral and Maxillotacial Surgery

  • Tissue Engineering

研究分野

  • ライフサイエンス / 口腔再生医学、歯科医用工学

  • ライフサイエンス / 補綴系歯学

経歴(researchmap)

  • 新潟大学大学院   医歯学総合研究科 生体組織再生工学   教授

    2013年6月 - 現在

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  • 新潟大学大学院   医歯学総合研究科 口腔解剖学   准教授

    2009年1月 - 2013年5月

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  • ミシガン大学   歯学部   Research Scientist/Clinical Assistant Professor

    2005年3月 - 2008年12月

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  • ミシガン大学   歯学部口腔外科学   Visiting Professor

    2002年7月 - 2005年2月

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  • 新潟大学大学院   医歯学総合研究科 組織再建口腔外科学   助教

    2001年4月 - 2005年2月

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  • ミシガン大学   歯学部口腔外科学   Visiting Professor

    1997年5月 - 1999年2月

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  • University of Michigan, visiting professor

    1997年 - 1999年

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  • 新潟大学   歯学部 第一口腔外科学   助手

    1992年7月 - 2001年3月

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  • 新潟大学歯学部付属病院   第一口腔外科学   医員

    1992年4月 - 1992年6月

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▶ 全件表示

経歴

  • 新潟大学   医歯学総合研究科 口腔生命科学専攻 摂食環境制御学   教授

    2013年6月 - 現在

  • 新潟大学   医歯学総合研究科 口腔生命科学専攻 摂食環境制御学   准教授

    2009年1月 - 2013年5月

  • 新潟大学   歯学部 歯学科   准教授

    2009年1月 - 2013年5月

学歴

  • 新潟大学   歯学研究科   口腔外科学

    - 1992年

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    国名: 日本国

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  • 新潟大学

    - 1992年

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  • 新潟大学   歯学部   歯学科

    - 1988年

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    国名: 日本国

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  • 新潟大学

    - 1988年

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所属学協会

 

論文

  • Manufacturing micropatterned collagen scaffolds with chemical-crosslinking for development of biomimetic tissue-engineered oral mucosa 査読

    Ayako Suzuki, Yoshihiro Kodama, Keito Miwa, Kazuma Kishimoto, Emi Hoshikawa, Kenta Haga, Taisuke Sato, Jun Mizuno, Kenji Izumi

    Scientific Reports   10 ( 1 )   2020年12月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    <title>Abstract</title>The junction between the epithelium and the underlying connective tissue undulates, constituting of rete ridges, which lack currently available soft tissue constructs. In this study, using a micro electro mechanical systems process and soft lithography, fifteen negative molds, with different dimensions and aspect ratios in grid- and pillar-type configurations, were designed and fabricated to create three-dimensional micropatterns and replicated onto fish-scale type I collagen scaffolds treated with chemical crosslinking. Image analyses showed the micropatterns were well-transferred onto the scaffold surfaces, showing the versatility of our manufacturing system. With the help of rheological test, the collagen scaffold manufactured in this study was confirmed to be an ideal gel and have visco-elastic features. As compared with our previous study, its mechanical and handling properties were improved by chemical cross-linking, which is beneficial for grafting and suturing into the complex structures of oral cavity. Histologic evaluation of a tissue-engineered oral mucosa showed the topographical microstructures of grid-type were well-preserved, rather than pillar-type, a well-stratified epithelial layer was regenerated on all scaffolds and the epithelial rete ridge-like structure was developed. As this three-dimensional microstructure is valuable for maintaining epithelial integrity, our micropatterned collagen scaffolds can be used not only intraorally but extraorally as a graft material for human use.

    DOI: 10.1038/s41598-020-79114-3

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    その他リンク: http://www.nature.com/articles/s41598-020-79114-3

  • Higher Accumulation of Docosahexaenoic Acid in the Vermilion of the Human Lip than in the Skin 査読 国際誌

    Md. Al Mamun, Shumpei Sato, Eiji Naru, Osamu Sakata, Emi Hoshikawa, Ayako Suzuki, Ariful Islam, Tomoaki Kahyo, Tomohito Sato, Takashi K. Ito, Makoto Horikawa, Reimu Fukui, Kenji Izumi, Mitsutoshi Setou

    International Journal of Molecular Sciences   21 ( 8 )   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The vermilion of the human lip is a unique facial area because of certain distinguishing features from the adjacent tissues such as the white lip (skin) and oral mucosa. However, the distinction in terms of molecular distribution between the vermilion and skin has remained unexplored. Therefore, we aimed to map the human lip by mass spectrometry imaging to gain understanding of the free fatty acid distribution in the vermilion. The lip specimens trimmed off during cheiloplasty were analyzed using desorption electrospray ionization-mass spectrometry imaging. Distributions of two monounsaturated fatty acids and three polyunsaturated fatty acids were observed in the human lip tissue: palmitoleic acid (POA) and oleic acid (OA) and linoleic acid (LA), arachidonic acid (AA), and docosahexaenoic acid (DHA), respectively. Although POA, OA, LA, and AA were differentially distributed across the vermilion and skin, DHA showed a higher accumulation in the epithelium of the vermilion compared to that in the skin. Our results clearly demonstrated the difference in fatty acid distributions between the vermilion and skin. The highly abundant DHA in the epithelium of the vermilion may have an antioxidant role and may thus protect the lip from aging. Our findings can provide a novel strategy for treating lip disorders.

    DOI: 10.3390/ijms21082807

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  • Development of microstructured fish scale collagen scaffolds to manufacture a tissue-engineered oral mucosa equivalent 査読 国際誌

    Kenji Izumi

    Journal of Biomaterials Science, Polymer Edition   31 ( 5 )   578 - 600   2020年1月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The present study aimed to develop a more biomimetic tissue-engineered oral mucosa equivalent comprising 1% type I tilapia scale collagen scaffold having microstructures mimicking the dermal-epidermal junction of oral mucosa and oral keratinocytes as graft materials for human use. We designed four micropattern prototypes mimicking the dermal-epidermal junction. Using a semiconductor process and soft lithography, negative molds were fabricated to develop microstructures using both polydimethylsiloxane and silicon substrates. Micropattern configurations of dermal-epidermal junctions manufactured from fish collagen consisting of a fibril network using our micropatterning system were well preserved, although the internal fibril network of the pillar pattern was sparse. Mixing 1% chondroitin sulfate with the collagen matrix minimized tissue-engineered oral mucosa equivalent contraction. Histologic examinations showed a flattening of the vertical dimensions of all microstructures and expansion of their pitches, indicating changes in the originally designed configurations. Nonetheless, histologic examinations revealed that a fully differentiated and stratified epithelial layer was developed on all scaffolds, suggesting that the microstructured fish scale collagen scaffolds have potential in the manufacturing of tissue-engineered oral mucosa equivalents for clinical use; however, enhancement of the mechanical properties of micropatterns is required. Our micropatterning technology can also apply to the development of oral mucosa in vitro models.

    DOI: 10.1080/09205063.2019.1706147

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  • Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine. 査読

    Kenji Izumi

    Journal of tissue engineering   10   2019年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening the proliferative capacity of cells in the early phases of cell culture were examined. We applied our software to movies converted from 96 consecutive time-lapse phase-contrast images of an oral keratinocyte culture. After segmenting the growing colonies, two indices were calculated based on each algorithm. The correlation between each index of the colonies and their proliferative capacity was evaluated. The software was able to assess cell/colony motion noninvasively, and each index reflected the observed cell kinetics. A positive linear correlation was found between cell/colony motion and proliferative capacity, indicating that both algorithms are potential tools for QC.

    DOI: 10.1177/2041731419881528

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  • Tissue Engineered Oral Mucosa 査読

    Izumi, K., Kato, H., Feinberg, S.E.

    Stem Cell Biology and Tissue Engineering in Dental Sciences   53   721 - 731   2015年

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Inc.  

    Oral mucosa primarily acts as a barrier against the external harmful environments. Loss of its barrier function due to diseases or injury will cause significant dysfunction within the oral cavity. Surgeons are frequently confronted with finding an acceptable source of autologous grafts for reconstruction of oral mucosa defects. Thus, there is a need to overcome these shortcomings of limited supply and donor site morbidity in the current surgical management/reconstruction of oral mucosa defects. Tissue engineering/regenerative medicine is an interdisciplinary field of developmental biology, life sciences, and engineering efforts that attempts to address challenges in the clinical arena. The understanding of the growth and functions of cells, the principles and methods of engineering, and the signals regulating cellular responses drives the fabrication of matrices and the design of tissue assembly to generate tissue-engineered products for in vivo and in vitro applications. Progress has been made over the years in the development of tissue-engineered substitutes that mimic human oral mucosa, either to be used as grafts for the replacement of mucosa defects, or for the in vitro oral mucosa models while tissue engineering of oral mucosa is still in its infancy. An increased understanding of stem cells, scaffolding, and signaling with extracellular matrix interactions will make its future possible. This chapter gives a comprehensive overview of the developments and future prospects of tissue-engineered constructs as oral mucosa substitutes for tissue repair and regeneration.

    DOI: 10.1016/B978-0-12-397157-9.00077-1

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  • Hypoxia induces an undifferentiated phenotype of oral keratinocytes in vitro 査読

    Kato, H., Izumi, K., Uenoyama, A., Shiomi, A., Kuo, S., Feinberg, S.E.

    Cells Tissues Organs   199 ( 5-6 )   393 - 404   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    The aim of this study was to determine the effects of hypoxia on the proliferating potential and phenotype of primary human oral keratinocytes cultured at ambient oxygen tension (20%) or at different levels of hypoxia (2 and 0.5% O-2). The effects of oxygen tensions on cellular metabolic activity, cell proliferation, clonogenicity and proliferation heterogeneity were measured. Cell cycle profiles were analyzed by a fluorescent-activated cell sorter, and p21(WAF1/CIP1) expression in the G(0)/G(1) phase was also concomitantly quantitated. The expression levels of cell cycle regulatory proteins were examined by immunoblotting, and the cellular senescence was assessed by senescence-associated beta-galactosidase staining. Basal and suprabasal keratinocyte phenotypes were determined by the expression levels of 14-3-3 sigma, p75(NTR) and alpha 6 integrin. Despite having a lower metabolism, the proliferation rate and clonogenic potential were remarkably enhanced in hypoxic cells. The significantly higher percentage of cells in the G(0)/G(1) phase under hypoxia and the expression patterns of cell cycle regulatory proteins in hypoxic cells were indicative of a state of cell cycle arrest in hypoxia. Furthermore, a decrease in the expression of p21(WAF1/CIP1) and p16(INK4A) and fewer beta-galactosidase-positive cells suggested a quiescent phenotype rather than a senescent one in hypoxic cells. Compared with normoxic cells, the differential expression patterns of keratinocyte phenotypic markers suggest that hypoxic cells that generate minimal reactive oxygen species, suppress the mammalian target of rapamycin activity and express hypoxia-inducible factor-1 alpha favor a basal cell phenotype. Thus, regardless of the predisposition to the state of cell cycle arrest, hypoxic conditions can maintain oral keratinocytes in vitro in an undifferentiated and quiescent state. (C) 2015 S. Karger AG, Basel

    DOI: 10.1159/000371342

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  • Intraoral Grafting of Tissue-Engineered Human Oral Mucosa 査読

    Kenji Izumi, Rodrigo F. Neiva, Stephen E. Feinberg

    INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS   28 ( 5 )   E295 - E303   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:QUINTESSENCE PUBLISHING CO INC  

    Purpose: The primary objective of this study was to evaluate the safety of a tissue-engineered human ex vivo-produced oral mucosa equivalent (EVPOME) in intraoral grafting procedures. The secondary objective was to assess the efficacy of the grafted EVPOME in producing a keratinized mucosal surface epithelium. Materials and Methods: Five patients who met the inclusion criteria of having one mucogingival defect or a lack of keratinized gingiva on a nonmolar tooth, along with radiographic evidence of sufficient interdental bone height, were recruited as subjects to increase the width of keratinized gingiva at the defect site. A punch biopsy specimen of the hard palate was taken to acquire oral keratinocytes, which were expanded, seeded, and cultured on an acellular dermal matrix for fabrication of an EVPOME. EVPOME grafts were applied directly over an intact periosteal bed and secured in place. At baseline (biopsy specimen retrieval) and at 7, 14, 30, 90, and 180 days postsurgery, Plaque Index and Gingival Index were recorded for each subject. In addition, probing depths, keratinized gingival width, and keratinized gingival thickness were recorded at baseline, 30, 90, and 180 days. Results: No complications or adverse reactions to EVPOME were observed in any subjects during the study. The mean gain in keratinized gingival width was 3 mm (range, 3 to 4 mm). The mean gain in keratinized gingival thickness was 1 mm (range, 1 to 2 mm). No significant changes in probing depths were observed. Conclusion: Based on these findings, it can be concluded that EVPOME is safe for intraoral use and has the ability to augment keratinized tissue around teeth. Future clinical trials are needed to further explore this potential.

    DOI: 10.11607/jomi.te11

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  • Cells/colony motion of oral keratinocytes determined by non-invasive and quantitative measurement using optical flow predicts epithelial regenerative capacity

    Emi Hoshikawa, Taisuke Sato, Kenta Haga, Ayako Suzuki, Ryota Kobayashi, Koichi Tabeta, Kenji Izumi

    Scientific Reports   11 ( 1 )   2021年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media {LLC}  

    DOI: 10.1038/s41598-021-89073-y

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  • The human vermilion surface contains a rich amount of cholesterol sulfate than the skin. 国際誌

    Md Al Mamun, Ariful Islam, Md Mahmudul Hasan, A S M Waliullah, Zinat Tamannaa, Do Huu Chi, Tomohito Sato, Tomoaki Kahyo, Kenji Kikushima, Yutaka Takahashi, Eiji Naru, Osamu Sakata, Mutsumi Yamanoi, Eri Kobayashi, Kenji Izumi, Tetsuya Honda, Yoshiki Tokura, Mitsutoshi Setou

    Journal of dermatological science   2021年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: The vermilion of the human lip presents characteristic features and undergoes aging faster than the skin. Therefore, knowledge of the vermilion surface-specific functional molecules is important to understand lip aging and formulate lip care products. Previously, we analyzed the free fatty acids distributions and showed that docosahexaenoic acid highly accumulated in the vermilion's epithelium than in the skin. OBJECTIVE: We aimed to explore the functional molecules other than the free fatty acids on the vermilion's surface. METHODS: Human lip tissues from children and tape-stripped samples from smooth and rough lips of adults were measured by desorption electrospray ionization-mass spectrometry imaging (DESI-MSI). RESULTS: DESI-MSI of children's lip sections revealed a major distribution of five phospholipid species in the viable layer, but not in the superficial area, of both the vermilion and the skin than that in the underlying tissue. Interestingly, a remarkably higher distribution of cholesterol sulfate was observed in the vermilion's superficial area compared to that in the skin in all subjects under this study. Furthermore, theDESI-MSI of tape-stripped lip sample showed an overall higher accumulation of cholesterol sulfate in the stratum corneum of the rough lip than that in the smooth lips showed an overall higher accumulation of cholesterol sulfate in the stratum corneum of the rough lips than that in the smooth lips. CONCLUSION: Our study concluded that cholesterol sulfate has a characteristic distribution to the vermilion's surface and showed an association with the roughness of the lip.

    DOI: 10.1016/j.jdermsci.2021.07.008

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  • HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma

    Akihiko Nakamura, Yoshito Kakihara, Akinori Funayama, Kenta Haga, Toshihiko Mikami, Daiki Kobayashi, Yutaka Yoshida, Kenji Izumi, Tadaharu Kobayashi, Makio Saeki

    Biochemical and Biophysical Research Communications   557   294 - 301   2021年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    DOI: 10.1016/j.bbrc.2021.04.021

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  • Perivascular Hedgehog responsive cells play a critical role in peripheral nerve regeneration via controlling angiogenesis

    Yurie Yamada, Jun Nihara, Supaluk Trakanant, Takehisa Kudo, Kenji Seo, Izumi Iida, Kenji Izumi, Masayuki Kurose, Yutaka Shimomura, Miho Terunuma, Takeyasu Maeda, Atsushi Ohazama

    Neuroscience Research   2021年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    DOI: 10.1016/j.neures.2021.06.003

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  • Identification and characterization of R2TP in the development of oral squamous cell carcinoma

    Tetsuo Kiguchi, Yoshito Kakihara, Manabu Yamazaki, Kouji Katsura, Kenji Izumi, Jun-ichi Tanuma, Takashi Saku, Ritsuo Takagi, Makio Saeki

    Biochemical and Biophysical Research Communications   548   161 - 166   2021年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    DOI: 10.1016/j.bbrc.2021.02.074

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  • Metabolomic Alteration of Oral Keratinocytes and Fibroblasts in Hypoxia

    Hiroko Kato, Masahiro Sugimoto, Ayame Enomoto, Miku Kaneko, Yuko Hara, Naoaki Saito, Aki Shiomi, Hisashi Ohnuki, Kenji Izumi

    Journal of Clinical Medicine   10 ( 6 )   1156 - 1156   2021年3月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:{MDPI} {AG}  

    DOI: 10.3390/jcm10061156

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  • 画像解析による口腔ケラチノサイトの細胞増殖能の非侵襲的評価

    干川 絵美, 佐藤 大祐, 鈴木 絢子, 羽賀 健太, 多部田 康一, 泉 健次

    新潟歯学会雑誌   50 ( 2 )   108 - 108   2020年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • ヒト3次元口唇インビトロモデル開発のための赤唇上皮特異的マーカーの検討

    泉 健次, 干川 絵美, 鈴木 絢子, 上野山 敦士, 瀬藤 光利, 福井 玲矛, Al Mamun Md., 華表 友暁, 高橋 豊, 成 英次, 坂田 修, 小林 エリ

    日本香粧品学会誌   44 ( 3 )   217 - 217   2020年9月

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    記述言語:日本語   出版者・発行元:日本香粧品学会  

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  • Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix 査読

    Kenji Izumi

    In Vitro Cellular & Developmental Biology - Animal   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s11626-020-00458-1

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  • Periodontal Ligament Cell Sheets and RGD-Modified Chitosan Improved Regeneration in the Horizontal Periodontal Defect Model 査読

    Kenji Izumi

    European Journal of Dentistry   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <jats:title>Abstract</jats:title><jats:p>
    Objective The aim of this study was to examine the potential of periodontal ligament (PDL) cells sheet and arginine-glycyl-aspartic acid (RGD)-modified chitosan scaffold for periodontal tissue regeneration in horizontal periodontal defect model.</jats:p><jats:p>
    Materials and Methods PDL cell cytotoxicity was tested with 3–[4,5- dimethylthiazol-2yl]–2,5-diphenyl-2H-tetrazolium bromide assay. Cell migration toward the chitosan-based materials was analyzed with trans-well migration assay. Horizontal periodontal defect model was created in four maxillary and mandibular lateral incisors of Macaque nemestrina. Following periodontal therapy, the sites were transplanted with various regenerative materials: (1) chitosan, (2) RGD-modified chitosan, (3) PDL cell sheet with chitosan, (4) PDL cell sheet with RGD-modified chitosan. The periodontal tissue regeneration was evaluated clinically and radiographically. Gingival crevicular fluids were collected each week to evaluate cementum protein-1 (CEMP-1) expression with enzyme-linked immunosorbent assay, while the biopsies were retrieved after 4 weeks for histological and microcomputed tomography evaluation.</jats:p><jats:p>
    Statistical Analysis Data was statistically analyzed using GraphPad Prism 6 for MacOS X. Normality was tested using the Shapiro–Wilk normality test. The Kruskal–Wallis test was used to compare the groups. Significance was accepted when p &lt; 0.05.</jats:p><jats:p>
    Results Clinical examination revealed more epithelial attachment was formed in the group with PDL cell sheet with RGD-modified chitosan. Similarly, digital subtraction radiography analysis showed higher gray scale, an indication of higher alveolar bone density surrounded the transplanted area, as well as higher CEMP-1 protein expression in this group. The incorporation of RGD peptide to chitosan scaffold in the group with or without PDL cells sheet reduced the distance of cement–enamel junction to the alveolar bone crest; hence, more periodontal tissue formed.</jats:p><jats:p>
    Conclusions Horizontal periodontal defect model could be successfully created in M. nemestrina model. Combination of PDL cell sheet and RGD-modified chitosan resulted in the higher potential for periodontal tissue regeneration. The results of this study highlight the PDL cell sheet and RGD-modified chitosan as a promising approach for future clinical use in periodontal regeneration.</jats:p>

    DOI: 10.1055/s-0040-1709955

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  • Rac1-dependent phagocytosis of apoptotic cells by oral squamous cell carcinoma cells: A possible driving force for tumor progression 査読 国際誌

    Kenji Izumi

    Experimental Cell Research   392 ( 1 )   112013 - 112013   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Apoptotic cell death frequently occurs in human cancer tissues including oral squamous cell carcinoma (SCC), wherein apoptotic tumor cells are phagocytosed not only by macrophages but also by neighboring tumor cells. We previously reported that the engulfment of apoptotic SCC cells by neighboring SCC cells frequently occurs at the invading front. Therefore, we hypothesized that the phagocytosis of these apoptotic cells by tumor cells contributes to disease progression. Herein, using cultured oral SCC cells, we aimed to confirm whether tumor cells actually phagocytose apoptotic cells and to examine whether cellular activities are regulated by the phagocytosis of apoptotic cells. Co-culture experiments showed that living cells could ingest apoptotic cells into phagolysosomes. NSC23766, an inhibitor of Rac1, which is a key regulator of phagocytic cup formation in professional phagocytes, dramatically suppressed the phagocytosis of apoptotic cells by living cells. Additionally, cell migration and the secretion of DKK1, a tumor-promoting protein, were enhanced by co-culture with apoptotic cells, whereas NSC23766 inhibited these effects. These results show that tumor cells can actively phagocytose apoptotic neighbors in a Rac1-dependent manner and that such activity increases their migration. The regulation of apoptotic cell phagocytosis thus represents new directions for therapeutic intervention for oral cancer.

    DOI: 10.1016/j.yexcr.2020.112013

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  • ROCK inhibitors enhance bone healing by promoting osteoclastic and osteoblastic differentiation 査読 国際誌

    Kenji Izumi

    Biochemical and Biophysical Research Communications   526 ( 3 )   547 - 552   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Osteoclast and osteoblast are essential for proper bone development and remodeling as well as recovery of bone fracture. In this study, we seek chemical compounds that enhance turnover of bone metabolism for promoting bone healing. First, we screen a chemical library which includes 378 compounds by using murine pre-osteoclastic RAW264.7 cells to identify compounds that promote osteoclastic differentiation. We find that two ROCK (Rho-associated coiled-coil kinase) inhibitors, HA-1077 (Fasudil) and Y-27632, enhance osteoclastogenesis. Subsequently, we identify that these two compounds also increase osteoblastic differentiation of MC3T3-E1 cells. Finally, our in vivo experiment shows that the local administration of ROCK inhibitors accelerate the bone healing of the rat calvarial defect.

    DOI: 10.1016/j.bbrc.2020.03.033

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  • M2 Phenotype Macrophages Colocalize with Schwann Cells in Human Dental Pulp 査読 国際誌

    Kenji Izumi

    Journal of Dental Research   論文受理 ( 印刷中 )   329 - 338   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <jats:p> Macrophages are immune cells with high plasticity that perform many functions related to tissue injury and repair. They are generally categorized as 2 functional phenotypes: M1 (proinflammatory) and M2 (anti-inflammatory and prohealing). To investigate the role of macrophages in human dental pulp, we examined the localization and distributional alterations of macrophages in healthy dental pulp as well as during the reparative process of pulp capping with mineral trioxide aggregate (MTA) and in cariously inflamed pulp of adult human teeth. We also quantified the populations of M1/M2 macrophages in healthy dental pulp by flow cytometric analysis. CD68<jats:sup>+</jats:sup>CD86<jats:sup>+</jats:sup> cells (M1 phenotype) and CD68<jats:sup>+</jats:sup>CD163<jats:sup>+</jats:sup> cells (M2 phenotype) were 2.11% ± 0.50% and 44.99% ± 2.22%, respectively, of 2.96% ± 0.41% CD68<jats:sup>+</jats:sup> cells (pan-macrophages) in whole healthy dental pulp. Interestingly, M2 phenotype macrophages were associated with Schwann cells in healthy pulp, during mineralized bridge formation, and in pulp with carious infections in vivo. Furthermore, the M2 macrophages associated with Schwann cells expressed brain-derived neurotrophic factor (BDNF) under all in vivo conditions. Moreover, we found that plasma cells expressed BDNF. Coculture of Schwann cells isolated from human dental pulp and human monocytic cell line THP-1 showed that Schwann cells induced M2 phenotypic polarization of THP-1 cell-derived macrophages. The THP-1 macrophages that maintained contact with Schwann cells were stimulated, leading to elongation of their cell shape and expression of M2 phenotype marker CD163 in cocultures. In summary, we revealed the spatiotemporal localization of macrophages and potent induction of the M2 phenotype by Schwann cells in human dental pulp. M2 macrophages protect neural elements, whereas M1 cells promote neuronal destruction. Therefore, suppressing the neurodestructive M1 phenotype and maintaining the neuroprotective M2 phenotype of macrophages by Schwann cells may be critical for development of effective treatment strategies to maintain the viability of highly innervated dental pulp. </jats:p>

    DOI: 10.1177/0022034519894957

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  • Biological reaction control using topography regulation of nanostructured titanium. 査読 国際誌

    Kenji Izumi

    Scientific reports   10 ( 1 )   2438 - 2438   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The micro- and nanosize surface topography of dental implants has been shown to affect the growth of surrounding cells. In this study, standardized and controlled periodic nanopatterns were fabricated with nanosized surface roughness on titanium substrates, and their influence on bone marrow stromal cells investigated. Cell proliferation assays revealed that the bare substrate with a 1.7 nm surface roughness has lower hydrophilicity but higher proliferation ability than that with a 0.6 nm surface roughness. Further, with the latter substrate, directional cell growth was observed for line and groove patterns with a width of 100 nm and a height of 50 or 100 nm, but not for those with a height of 10 or 25 nm. With the smooth substrate, time-lapse microscopic analyses showed that more than 80% of the bone marrow cells on the line and groove pattern with a height of 100 nm grew and divided along the lines. As the nanosized grain structure controls the cell proliferation rate and the nanosized line and groove structure (50-100 nm) controls cell migration, division, and growth orientation, these standardized nanosized titanium structures can be used to elucidate the mechanisms by which surface topography regulates tissue responses to biomaterials.

    DOI: 10.1038/s41598-020-59395-4

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  • がん関連線維芽細胞は口腔扁平上皮癌においてSOX9発現を増強させ浸潤を促進する

    羽賀 健太, 山崎 学, 丸山 智, 鈴木 絢子, 干川 絵美, 船山 昭典, 三上 俊彦, 小林 正治, 泉 健次, 田沼 順一

    新潟歯学会雑誌   49 ( 2 )   86 - 86   2019年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • ヒト歯髄においてシュワン細胞はマクロファージをM2型へ転換する

    吉羽 永子, 大倉 直人, 前川 知樹, 泉 健次, 細矢 明宏, 中村 浩彰, 前田 健康, 野杁 由一郎, 吉羽 邦彦, 枝並 直樹, 遠間 愛子

    Journal of Oral Biosciences Supplement   2019   302 - 302   2019年10月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 紫外線光重合開始剤を含むMMAにより前処理したPEEKの接着強さ

    大川 成剛, 木村 龍弥, 高 昇将, 青柳 裕仁, 泉 健次

    日本歯科理工学会誌   38 ( Special Issue74 )   69 - 69   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科理工学会  

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  • 化学修飾によるPEEK表面の改質とその接着特性

    大川 成剛, 高 昇将, 木村 龍弥, 青柳 裕仁, 泉 健次

    日本歯科理工学会誌   38 ( Special Issue73 )   75 - 75   2019年4月

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  • Evaluation of a Newly Designed Microperforated Pure Titanium Membrane for Guided Bone Regeneration. 査読

    Kenji Izumi

    The International journal of oral & maxillofacial implants   34 ( 2 )   411 - 422   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Quintessence Publishing  

    PURPOSE:This study aimed to evaluate the safety and efficacy of newly designed, laser-perforated pure titanium membranes for guided bone regeneration and to compare them with an existing product, the FRIOS BoneShield (FBS). MATERIALS AND METHODS:Acute-type lateral ridge defects were bilaterally created in the mandibles of 13 dogs (two defects per animal). The defects were randomly divided into three groups and were reconstructed with particulate autologous bone (PAB) in combination with three different titanium membranes: (1) F001M0 (prototype without a frame), (2) F001M1 (prototype with a frame), and (3) FBS as a standard membrane. All animals were observed periodically and sacrificed 26 or 27 weeks postoperatively. At 26 weeks, approximately half of the dogs in each group underwent membrane removal to examine the postoperative condition of the titanium membranes. The samples were dissected and processed for radiographic, histologic, and histomorphometric analyses. RESULTS:Membrane exposure was not found in the F001M0 or F001M1 groups, and their membranes were removed easily without adhesion to the surrounding tissue. Regenerated bone tissue volume was largest in the F001M1 group, followed by the F001M0 and FBS groups. A significant difference was observed only between the F001M1 and FBS groups (P = .047). In contrast, bone mineral density was similar among the three groups. Histologically, a layer of fibrous tissue was present underneath the titanium membrane, overlying the regenerated cortical bone in all the groups. Notably, the tissue was highly vascular in the F001M1 and F001M0 groups compared with the FBS group. In addition, there was little difference in the semiquantitative soft tissue evaluation and histologic findings of bone regeneration among the three groups. The histomorphometric analysis revealed that the regenerated bone area was larger in the F001M1 and F001M0 groups than in the FBS group, and a significant difference was observed only between the F001M1 and FBS groups (P = .045). Calcific osseous area was similar among the three groups. CONCLUSION:The safety and efficacy of both F001M0 and F001M1 were equivalent to or greater than those of FBS, thereby indicating their potential for future clinical applications.

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  • Evaluation of a newly designed microperforated titanium membrane with beta-tricalcium phosphate for guided bone regeneration in dog mandibles 査読

    Hasegawa, H., Kaneko, T., Kanno, C., Endo, M., Akimoto, T., Yamazaki, M., Kitabatake, T., Masui, S., Ishihata, H., Izumi, K.

    International Journal of Oral and Maxillofacial Implants   34 ( 5 )   1132 - 1142   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Quintessence Publishing  

    PURPOSE:This study evaluated the efficacy of newly designed, laser-perforated pure titanium membranes for guided bone regeneration using beta-tricalcium phosphate (β-TCP), and compared them with the existing membrane. MATERIALS AND METHODS:Bilateral acute lateral ridge defects were created in the mandibles of 12 dogs (four defects per animal), which were then randomly divided into two groups (six dogs each). The twenty-four bone defects in each group were then further divided into five groups. The groups were as follows: (1) F001M0, a prototype membrane without a frame plus β-TCP (n = 5); (2) F001M1, a prototype membrane with a frame plus β-TCP (n = 5); (3) FBS, an existing control membrane plus β-TCP (n = 5); (4) control 1, β-TCP without membrane and with covering flap only (n = 5); and (5) control 2, no treatment (no β-TCP and no membrane) (n = 4). In all groups where β-TCP was used, it was mixed with peripheral blood. The animals were necropsied at 6 or 12 weeks postoperatively (six dogs each), and samples were collected and processed for radiographic, histologic, and histomorphometric analyses. RESULTS:Among the three membrane groups, regenerated tissue and bone volume was greatest in the F001M1 group at both 6 and 12 weeks postoperatively, although differences among groups were not statistically significant. Bone mineral density was similar among the membrane groups. Histologic analysis revealed that immature fibroblasts were present on the laser-perforated portion at 6 weeks, which induced vascularization. In addition, more calcified bone was replaced beneath the prototypes than beneath the FBS membrane at 12 weeks. Histomorphometric analyses revealed that the calcific osseous areas at 12 weeks after surgery were significantly greater in the F001M1 and F001M0 groups than in the FBS group (P = .021, P = .032). Furthermore, the fibrous tissue areas beneath the membrane at 12 weeks postoperatively were significantly smaller in the prototype groups than in the FBS group (P = .02, P = .02). CONCLUSION:The efficacies of both prototype membranes were not inferior to that of the FBS membrane, indicating that they may facilitate bone regeneration and maturation when β-TCP mixed with autologous blood is employed.

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  • 表面改質した繊維強化PEEKの接着特性

    大川 成剛, 高 昇将, 木村 龍弥, 青柳 裕仁, 泉 健次

    日本歯科理工学会誌   37 ( Special Issue72 )   85 - 85   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科理工学会  

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  • 大気プラズマ処理したPEEKの接着特性

    大川 成剛, 高 昇将, 青柳 裕仁, 泉 健次

    日本歯科理工学会誌   37 ( Special Issue71 )   32 - 32   2018年3月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科理工学会  

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  • Deformation characteristics of cultured single viable cells by squeezing tests 査読

    Ishii, H., Ishii, T., Narumi, T., Izumi, K., Ushida, A., Sato, T.

    Nihon Reoroji Gakkaishi   46 ( 4 )   179 - 184   2018年

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1678/rheology.46.179

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  • 紫外線によって表面改質したPEEKの接着特性について

    大川 成剛, 高 昇将, 青柳 裕仁, 泉 健次

    日本歯科理工学会誌   36 ( 5 )   326 - 326   2017年9月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科理工学会  

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  • Study on deformation characteristics of single cells by squeezing test

    Haruhiko Ishii, Tatsuji Ishii, Takatsune Narumi, Kenji Izumi, Akiomi Ushida

    Proceedings of The XVIIth International Congress on Rheology, ICR2016   ( 5706 )   2016年8月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

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  • Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model 査読

    Uenoyama, A., Kakizaki, I., Shiomi, A., Saito, N., Hara, Y., Saito, T., Ohnuki, H., Kato, H., Takagi, R., Maeda, T., Izumi, K.I.

    Bioscience, Biotechnology and Biochemistry   80 ( 7 )   1344 - 1355   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting. This study investigated the effects of one of the C-xylopyranoside derivatives, -D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration. Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling. Compared with the control, 2mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness. It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin 6, CD44, and Akt/mTOR signaling substrates. These findings suggest that XPP supplementation contributes to consistent epithelial regeneration. Moreover, upregulation of those markers may play a role in increasing the quality of the oral mucosal epithelium.

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  • Cyclic mechanical pressure-loading alters epithelial homeostasis in a three-dimensional in vitro oral mucosa model: Clinical implications for denture-wearers 査読

    Shiomi, A., Izumi, K., Uenoyama, A., Saito, T., Saito, N., Ohnuki, H., Kato, H., Kanatani, M., Nomura, S., Egusa, H., Maeda, T.

    Journal of Oral Rehabilitation   42 ( 3 )   192 - 201   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Denture-wearing affects the quality and quantity of epithelial cells in the underlying healthy oral mucosa. The physiologic mechanisms, however, are poorly understood. This study aimed to compare histologic changes and cellular responses of an epithelial cell layer to cyclic mechanical pressure-loading mimicking denture-wearing using an organotypic culture system to develop a three-dimensional in vitro oral mucosa model (3DOMM). Primary human oral keratinocytes and fibroblasts were serially grown in a monolayer culture, and cell viability was measured under continuous cyclic mechanical pressure (50kPa) for 7days (cycles of 60min on, 20s off to degas and inject air). Upon initiation of an air-liquid interface culture for epithelial stratification, the cyclic pressure, set to the mode above mentioned, was applied to the 3DOMMs for 7days. Paraffin-embedded 3DOMMs were examined histologically and immunohistochemically. In the monolayer culture, the pressure did not affect the viability of oral keratinocytes or fibroblasts. Few histologic changes were observed in the epithelial layer of the control and pressure-loaded 3DOMMs. Immunohistochemical examination, however, revealed a significant decrease in Ki-67 labelling and an increase in filaggrin and involucrin expression in the suprabasal layer of the pressure-loaded 3DOMMs. Pressure-loading attenuated integrin 1 expression and increased matrix metalloproteinase-9 activity. Incomplete deposition of laminin and type IV collagen beneath the basal cells was observed only in the pressure-loaded 3DOMM. Cyclic pressure-loading appeared to disrupt multiple functions of the basal cells in the 3DOMM, resulting in a predisposition towards terminal differentiation. Thus, denture-wearing could compromise oral epithelial homeostasis.

    DOI: 10.1111/joor.12254

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  • Zoledronic acid impairs re-epithelialization through down-regulation of integrin αvβ6 and transforming growth factor beta signalling in a three-dimensional in vitro wound healing model. 査読

    Saito T, Izumi K, Shiomi A, Uenoyama A, Ohnuki H, Kato H, Terada M, Nozawa-Inoue K, Kawano Y, Takagi R, Maeda T

    Int J Oral Maxillofac Surg   43 ( 3 )   373 - 380   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.ijom.2013.06.016

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  • Distinct expression patterns and roles of aldehyde dehydrogenases in normal oral mucosa keratinocytes: differential inhibitory effects of a pharmacological inhibitor and RNAi-mediated knockdown on cellular phenotype and epithelial morphology 査読

    Hiroko Kato, Kenji Izumi, Taro Saito, Hisashi Ohnuki, Michiko Terada, Yoshiro Kawano, Kayoko Nozawa-Inoue, Chikara Saito, Takeyasu Maeda

    HISTOCHEMISTRY AND CELL BIOLOGY   139 ( 6 )   847 - 862   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Aldehyde dehydrogenases (ALDHs), enzymes responsible for detoxification and retinoic acid biosynthesis, are considered a potent functional stem cell marker of normal and malignant cells in many tissues. To date, however, there are no available data on ALDH distributions and functions in oral mucosa. This study aims to clarify the levels and types of ALDH expression using immunohistochemistry with accompanying mRNA expression as well as an ALDEFLUOR assay, and to assess phenotypic and histological changes after manipulation of the ALDH activity of oral keratinocytes to increase the potency of a tissue-engineered oral mucosa by a specific ALDH inhibitor, diethylaminobenzaldehyde (DEAB), together with small interfering RNA of ALDH1A3 and ALDH3A1. Results showed the mRNA and cytoplasmic protein expression of ALDH1A3 and ALDH3A1 to be mostly localized in the upper suprabasal layer although no ALDH1A1 immunoreaction was detected throughout the epithelium. Oral keratinocytes with high ALDH activity exhibited a profile of differentiating cells. By pharmacological inhibition, the phenotypic analysis revealed the proliferating cell-population shifting to a more quiescent state compared with untreated cells. Furthermore, a well-structured epithelial layer showing a normal differentiation pattern and a decrease in Ki-67 immunopositive basal cells was developed by DEAB incubation, suggesting a slower turnover rate efficient to maintain undifferentiated cells. Histological findings of a regenerated oral epithelium by ALDH1A3 siRNA were similar to those when treated with DEAB while ALDH3A1 siRNA eradicated the epithelial regenerative capacity. These observations suggest the effects of phenotypic and morphological alterations by DEAB on oral keratinocytes are mainly consequent to the inhibition of ALDH1A3 activity.

    DOI: 10.1007/s00418-012-1064-7

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  • Tissue-engineered constructs of human oral mucosa examined by raman spectroscopy 査読

    Alexander Khmaladze, Arindam Ganguly, Shiuhyang Kuo, Mekhala Raghavan, Raghu Kainkaryam, Jacqueline H. Cole, Kenji Izumi, Cynthia L. Marcelo, Stephen E. Feinberg, Michael D. Morris

    Tissue Engineering - Part C: Methods   19 ( 4 )   299 - 306   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Mary Ann Liebert Inc.  

    A noninvasive quality monitoring of tissue-engineered constructs is a required component of any successful tissue-engineering technique. During a 2-week production period, ex vivo produced oral mucosa-equivalent constructs (EVPOMEs) may encounter adverse culturing conditions that might compromise their quality and render them ineffective. We demonstrate the application of near-infrared Raman spectroscopy to in vitro monitoring of EVPOMEs during their manufacturing process, with the ultimate goal of applying this technology in situ to monitor the grafted EVPOMEs. We identify Raman spectroscopic failure indicators for less-than optimal EVPOMEs that are stressed by higher temperature and exposure to higher than normal concentration of calcium ions. Raman spectra of EVPOMEs exposed to thermal and calcium stress showed correlation of the band height ratio of CH2 deformation to phenylalanine ring breathing modes, providing a Raman metric to distinguish between viable and nonviable constructs. We compared these results to histology and glucose consumption measurements, demonstrating that Raman spectroscopy is more sensitive and specific to changes in proteins' secondary structure not visible by H&amp
    E histology. We also exposed the EVPOMEs to rapamycin, a cell growth inhibitor and cell proliferation capacity preserver, and distinguished between EVPOMEs pretreated with 2 nM rapamycin and controls, using the ratio of the Amide III envelope to the phenylalanine band as an indicator. © 2013, Mary Ann Liebert, Inc.

    DOI: 10.1089/ten.tec.2012.0287

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  • Enrichment of oral mucosa and skin keratinocyte progenitor/stem cells 査読

    Kenji Izumi, Cynthia L. Marcelo, Stephen E. Feinberg

    Methods in Molecular Biology   989   293 - 303   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The isolation of human oral mucosa/skin keratinocytes progenitor/stem cells is clinically important to regenerate epithelial tissues for the treatment of oral mucosa/skin defects. Researchers have attempted to isolate a keratinocyte progenitor/stem cell population using cell markers, rapid adherence to collagen type IV, and other methods. In this regard, one of the specific characteristics of keratinocyte progenitor/stem cells is that these cells have a smaller diameter than differentiated cells. This chapter describes methods used in our laboratory to set up primary human oral mucosa and skin keratinocytes in a chemically defined culture system devoid of animal derived products. We utilized the cells in a FDA-approved human clinical trial that involved the intraoral grafting of an ex vivo produced oral mucosa equivalent to increase keratinized tissue around teeth. We also provide two protocols on how to sort keratinocytes using physical criterion, cell size, using a cell sorter and a serial filtration system. © 2013 Springer Science+Business Media New York.

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  • Construction and characterization of a tissue-engineered oral mucosa equivalent based on a chitosan-fish scale collagen composite 査読

    Michiko Terada, Kenji Izumi, Hisashi Ohnuki, Taro Saito, Hiroko Kato, Marie Yamamoto, Yoshiro Kawano, Kayoko Nozawa-Inoue, Haruhiko Kashiwazaki, Toshiyuki Ikoma, Junzo Tanaka, Takeyasu Maeda

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS   100B ( 7 )   1792 - 1802   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    This study was designed to (1) assess the in vitro biocompatibility of a chitosancollagen composite scaffold (C3) constructed by blending commercial chitosan and tilapia scale collagen with oral mucosa keratinocytes, (2) histologically and immunohistochemically characterize an ex vivo-produced oral mucosa equivalent constructed using the C3 (EVPOME-C), and (3) compare EVPOME-C with oral mucosa constructs utilizing AlloDerm (R) (EVPOME-A), BioMend (R) Extend (TM) (EVPOME-B), and native oral mucosa. C3 scaffold had a well-developed fibril network and a sufficiently small porosity to prevent keratinocytes from growing inside the scaffold after cell-seeding. The EVPOME oral mucosa constructs were fabricated in a chemically defined culture system. After culture at an air-liquid interface, EVPOME-C and EVPOME-B had multilayered epithelium with keratinization, while EVPOME-A had a more organized stratified epithelium. Ki-67 and p63 immunolabeled cells in the basal layer of all EVPOMEs suggested a regenerative ability. Compared with native oral mucosa, the keratin 15 and 10/13 expression patterns in all EVPOMEs showed a less-organized differentiation pattern. In contrast to the beta 1-integrin and laminin distribution in EVPOME-A and native oral mucosa, the subcellular deposition in EVPOME-C and EVPOME-B indicated that complete basement membrane formation failed. These findings demonstrated that C3 has a potential application for epithelial tissue engineering and provides a new potential therapeutic device for oral mucosa regenerative medicine. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.

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  • Zoledronic acid induces S-phase arrest via a DNA damage response in normal human oral keratinocytes 査読

    Hisashi Ohnuki, Kenji Izumi, Michiko Terada, Taro Saito, Hiroko Kato, Akiko Suzuki, Yoshiro Kawano, Kayoko Nozawa-Inoue, Ritsuo Takagi, Takeyasu Maeda

    ARCHIVES OF ORAL BIOLOGY   57 ( 7 )   906 - 917   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: This study aimed to clarify the effects of zoledronic acid (ZOL) on human primary oral mucosal keratinocytes growing in a monolayer culture and on a tissue-engineered oral mucosal construct.
    Design: Changes in the viability and proliferation of oral keratinocytes incubated with ZOL were measured. Following treatment with 10 mu M ZOL, histological examinations and immunohistochemical analyses for Ki-67, Geminin, and phospho-histone (gamma)-H2A.X were performed on tissue-engineered oral mucosa. Cell cycle distribution and the degree of apoptosis were also measured by flow cytometry. Additionally, we measured the expression of cell cycle regulatory proteins as well as phospho-Chk1 and -Chk2.
    Results: ZOL treatment suppressed cell viability and proliferation in a dose-dependent manner. Compared with untreated tissue-engineered oral mucosa, ZOL treatment resulted in a thinner epithelium in which the basal cells appeared less-organised. This is consistent with the observed significant reduction in the Ki-67 labelling index. In contrast, the Geminin labelling index was significantly higher than that in the untreated sample. In spite of the presence of a few apoptotic cells, ZOL induced an arrest in S-phase, which was confirmed by our observed alterations in the expression levels of cyclin A, B1, p27(KIP1), Rb and phospho-Rb. When the proteasome inhibitor MG132 was added to the ZOL-treated cells, we observed a partial restoration of the expression of cyclin A, cyclin B1, and p27(KIP1). Expression of phospho-Chk1 was detected, and a significant increase in the labelling index of gamma-H2A.X was also seen.
    Conclusions: These results indicate that a 10-mu M ZOL treatment induces a DNA damage response in oral keratinocytes that activates the ubiguitin-mediated proteolysis of cell cycle regulators, resulting in cell cycle arrest and repressive effects on cell viability, proliferation, and epithelial turnover. (C) 2011 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.archoralbio.2011.11.015

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  • Raman spectroscopic analysis of human tissue engineered oral mucosa constructs (EVPOME) perturbed by physical and biochemical methods 査読

    Alexander Khmaladze, Arindam Ganguly, Mekhala Raghavan, Shiuhyang Kuo, Jacqueline H. Cole, Cynthia L. Marcelo, Stephen E. Feinberg, Kenji Izumi, Michael D. Morris

    Progress in Biomedical Optics and Imaging - Proceedings of SPIE   8219   2012年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    We show the application of near-infrared Raman Spectroscopy to in-vitro monitoring of the viability of tissue constructs (EVPOMEs). During their two week production period EVPOME may encounter thermal, chemical or biochemical stresses that could cause development to cease, rendering the affected constructs useless. We discuss the development of a Raman spectroscopic technique to study EVPOMEs noninvasively, with the ultimate goal of applying it in-vivo. We identify Raman spectroscopic failure indicators for EVPOMEs, which are stressed by temperature, and discuss the implications of varying calcium concentration and pre-treatment of the human keratinocytes with Rapamycin. In particular, Raman spectra show correlation of the peak height ratios of CH 2 deformation to phenylalanine ring breathing, providing a Raman metric to distinguish between viable and nonviable constructs. We also show the results of singular value decomposition analysis, demonstrating the applicability of Raman spectroscopic technique to both distinguish between stressed and non-stressed EVPOME constructs, as well as between EVPOMEs and bare AlloDerm substrates, on which the oral keratinocytes have been cultured. We also discuss complications arising from non-uniform thickness of the AlloDerm® substrate and the cultured constructs, as well as sampling protocols used to detect local stress and other problems that may be encountered in the constructs. © 2012 SPIE.

    DOI: 10.1117/12.908281

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  • Raman Spectroscopic Analysis of Human Tissue Engineered Oral Mucosa Constructs (EVPOME) Perturbed by Physical and Biochemical Methods 査読

    Alexander Khmaladze, Arindam Ganguly, Mekhala Raghavan, Shiuhyang Kuo, Jacqueline H. Cole, Cynthia L. Marcelo, Stephen E. Feinberg, Kenji Izumi, Michael D. Morris

    BIOMEDICAL VIBRATIONAL SPECTROSCOPY V: ADVANCES IN RESEARCH AND INDUSTRY   8219   2012年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:SPIE-INT SOC OPTICAL ENGINEERING  

    We show the application of near-infrared Raman Spectroscopy to in-vitro monitoring of the viability of tissue constructs (EVPOMEs). During their two week production period EVPOME may encounter thermal, chemical or biochemical stresses that could cause development to cease, rendering the affected constructs useless. We discuss the development of a Raman spectroscopic technique to study EVPOMEs noninvasively, with the ultimate goal of applying it in-vivo. We identify Raman spectroscopic failure indicators for EVPOMEs, which are stressed by temperature, and discuss the implications of varying calcium concentration and pre-treatment of the human keratinocytes with Rapamycin. In particular, Raman spectra show correlation of the peak height ratios of CH2 deformation to phenylalanine ring breathing, providing a Raman metric to distinguish between viable and nonviable constructs. We also show the results of singular value decomposition analysis, demonstrating the applicability of Raman spectroscopic technique to both distinguish between stressed and non-stressed EVPOME constructs, as well as between EVPOMEs and bare AlloDerm (R) substrates, on which the oral keratinocytes have been cultured. We also discuss complications arising from non-uniform thickness of the AlloDerm (R) substrate and the cultured constructs, as well as sampling protocols used to detect local stress and other problems that may be encountered in the constructs.

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  • Tissue Engineering 査読

    Miller H. Smith, Kenji Izumi, Stephen E. Feinberg

    Current Therapy in Oral and Maxillofacial Surgery   79 - 91   2012年

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Elsevier Inc.  

    DOI: 10.1016/B978-1-4160-2527-6.00009-8

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  • COMPARISON OF SCANNING ACOUSTIC MICROSCOPY AND HISTOLOGY IMAGES IN CHARACTERIZING SURFACE IRREGULARITIES AMONG ENGINEERED HUMAN ORAL MUCOSAL TISSUES 査読

    Frank Winterroth, Kyle W. Hollman, Shiuhyang Kuo, Kenji Izumi, Stephen E. Feinberg, Scott J. Hollister, J. Brian Fowlkes

    ULTRASOUND IN MEDICINE AND BIOLOGY   37 ( 10 )   1734 - 1742   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Acoustic microscopy was used to monitor an ex vivo produced oral mucosal equivalent (EVPOME) developed on acellular cadaveric dermis (AlloDerm (R)). As seeded cells adhered and grew, they filled in and smoothed out the surface irregularities, followed by the production of a keratinized protective outermost layer. If noninvasive in vitro ultrasonic monitoring of these cellular changes could be developed, then tissue cultivation could be adjusted in-process to account for biologic variations in the development of these stratified cell layers. Cultured keratinocytes (from freshly obtained oral mucosa) were harvested and seeded onto AlloDerm (R) coated with human type IV collagen and cultured 11 days. EVPOMEs were imaged on the 11th day post-seeding using a scanning acoustic microscope (SAM) that consists of a single-element transducer: 61 MHz center frequency, 32 MHz bandwidth, 1.52 f-number. The specimen surface was determined by thresholding the magnitude of the signal at the first axial incidence of a value safely above noise: 20-40 dB above the signal for the water and 2-dimensional (2-D) ultrasonic images were created using confocal image reconstruction. A known area from each micrograph was divided into 12-40 even segments and examined for surface irregularities. These irregularities were quantified and one-way analysis of variance (ANOVA) and linear regression analysis were performed to correlate the surface profiles for both the AlloDerm (R) and EVPOME specimens imaged by SAM. Histology micrographs of the AlloDerm (R) and EVPOME specimens were also prepared and examined for surface irregularities. Unseeded AlloDerm (R) averaged seven to nine surface changes per 400 mu m. The number of changes in surface irregularities decreased to two to three per 400 mu m on the mature EVPOMEs. The numbers of surface irregularities between the unseeded AlloDerm (R) vs. developing EVPOME are similar for both histology and SAM 2-D B-scan images. For the EVPOME 2-D B-scan micrographs produced by SAM, the decrease in surface irregularities is indicative of the stratified epithelium formed by seeded oral keratinocytes; verified in the histology images between the AlloDerm (R) and EVPOME. A near 1: 1 linear correlation shows the similarities between the two imaging modalities. SAM demonstrates its ability to discern the cell development and differentiation occurring on the EVPOME devices. Unlike histology, SAM measurements are noninvasive and can be used to monitor tissue graft development without damaging any cells/tissues. (E-mail: fwinterr@umich.edu) (C) 2011 World Federation for Ultrasound in Medicine & Biology.

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  • Raman spectroscopy monitoring of the cellular activities of a tissue‐engineered ex vivo produced oral mucosal equivalent 査読

    Lo W-L, Lai J-Y, Feinberg SE, Izumi K, Kao S-Y, Chang C-S, Lin A, Chiang HK

    Journal of Raman Spectroscopy   42 ( 2 )   174 - 178   2011年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/jrs.2688

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  • Detection of acid-sensing ion channel 3 (ASIC3) in periodontal Ruffini endings of mouse incisors 査読

    Farhana Rahman, Fumiko Harada, Isao Saito, Akiko Suzuki, Yoshiro Kawano, Kenji Izumi, Kayoko Nozawa-Inoue, Takeyasu Maeda

    NEUROSCIENCE LETTERS   488 ( 2 )   173 - 177   2011年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    The acid-sensing ion channel 3 (ASIC3), a member of the epithelial sodium channel/degenerin (ENaC/DEG) superfamily, has been reported to participate in acid sensing, mechanosensation, and nociception. However, no information is available regarding the precise localization and function of this molecule in the periodontal ligament, which contains abundant sensory nerves originating from the trigeminal ganglion. The present study examined the expression of ASIC3 in the lingual periodontal ligament of mouse incisors by immunohistochemistry. Furthermore, the expression of ASIC3 in the trigeminal ganglion which innervates the periodontal ligament - was investigated at protein (immunohistochemistry and quantitative analysis) and mRNA levels (RT-PCR technique and in situ hybridization histochemistry). Immunohistochemistry for ASIC3 was able to demonstrate dendritic profiles of the periodontal Ruffini endings in the mouse incisors. No thin fibers terminating as nociceptive free nerve endings exhibited ASIC3 immunoreactivity. Double immunofluorescent staining revealed ASIC3 immunoreaction in the axoplasm but not in the ordinary Schwann cells - including the associated terminal Schwann cells. Observation of the trigeminal ganglia showed variously sized neurons expressing ASIC3 immunoreaction; the most intense immunopositivity was found in the small and medium-sized neurons, as confirmed by in situ hybridization histochemistry using a specific cRNA probe. Quantitative analysis on trigeminal ganglion neurons showed that 38.0% of ASIC3 neurons could be categorized as medium-sized neurons which mediate mechanotransduction. These findings suggest that ASIC3 functions as a molecule for mechanosensation in the periodontal Ruffini endings. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2010.11.023

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  • 培養複合口腔粘膜移植を応用した口唇口蓋裂の2例

    飯田 明彦, 芳澤 享子, 小山 貴寛, 齋藤 太郎, 高木 律男, 齊藤 力, 齋藤 功, 小野 和宏, 泉 健次

    日本口蓋裂学会雑誌   35 ( 3 )   235 - 240   2010年10月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本口蓋裂学会  

    口唇裂・口蓋裂手術時の口腔粘膜組織欠損に対し,培養複合口腔粘膜(&lt;I&gt;Ex Vivo&lt;/I&gt; Produced Oral Mucosa Equivalent: EVPOME)の移植を行った2例を報告した。&lt;br&gt;EVPOMEは培養した自家口腔粘膜上皮細胞を無細胞性ヒト新鮮屍体真皮であるAlloDerm&lt;sup&gt;&amp;reg;&lt;/sup&gt;上に播種し一体化させたもので,組織構造は全層粘膜移植材と同等である。すなわち,従来の培養粘膜上皮シートでは得られなかった物理的強度が得られ,手術の操作性が向上した。また,細胞培養システムも,マウスfeeder layerや,ウシ血清等の動物由来細胞/因子を含まないので安全性が確保されている利点もある。&lt;br&gt;症例1:両側性唇顎口蓋裂。硬口蓋正中部の瘻孔閉鎖を,口蓋動脈を含む有茎弁で行い,その結果生じた新鮮創面に対し,EVPOMEを移植した。&lt;br&gt;症例2:両側性唇顎口蓋裂。顎間骨部の口腔前庭拡張術を行い,生じた新鮮創面に対し,EVPOMEを移植した。&lt;br&gt;いずれの症例も,移植床周囲の瘢痕組織に縫合したが,縫合は容易であった。移植材は術後1週までに完全生着し,有害事象も生じなかった。&lt;br&gt;口唇裂・口蓋裂のような組織欠損や瘢痕組織を有する患者へのEVPOME移植は有用であると思われた。今後は適応拡大のため,口唇裂・口蓋裂児への適応を念頭に置いた凍結保存細胞によるEVPOMEの臨床応用や,AlloDerm&lt;sup&gt;&amp;reg;&lt;/sup&gt;に代わる安定供給可能なスキャホールドの開発などを行っていくべきであると考えられた。

    DOI: 10.11224/cleftpalate.35.235

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  • Isolation of small-sized human epidermal progenitor/stem cells by Gravity Assisted Cell Sorting (GACS) 査読

    Yasushi Fujimori, Kenji Izumi, Stephen E. Feinberg, Cynthia L. Marcelo

    JOURNAL OF DERMATOLOGICAL SCIENCE   56 ( 3 )   181 - 187   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Background: Small diameter characterizes epidermal progenitor/stem cells. We have developed Gravity Assisted Cell Sorting (GACS) to simply enrich small-sized epidermal progenitor/stem cells.
    Objective: The cells sorted by GACS were characterized by fluorescence-activated cell sorting analysis, and cultured for up to 7 weeks. The cultured cells were then used for reconstruction of skin equivalent.
    Methods: GACS was performed on primary cultures (primary cell) and passage 6-7 cultures (cultured cell) of keratinocytes. A keratinocyte suspension was sized into two groups: cells trapped by a 20 mu m filter (trapped cells), and cells flowing through both a 20 and 11 mu m filter (non-trapped cells).
    Results: In the primary cell groups, viability of the trapped cells was 62.5 +/- 7.2% compared to 77.0 +/- 3.7% for the non-trapped cells. In the cultured cell groups, viability of the trapped cells was 64.3 +/- 14.9%, compared to the non-trapped cells (93.1 +/- 2.0%). Flow cytometric analysis showed better discrimination by cell size between trapped and non-trapped cells in culture than in the primary cell suspension. Non-trapped cells contained a larger number of cells with high levels of alpha 6 integrin and low levels of CD71 (alpha 6 integrin(bri)CD71(dim),), indicating an enriched progenitor/stem cell population. The difference in these markers between the non-trapped and trapped cells was seen in both the primary and cultured cell groups although this difference was more distinct in cultured cells. Culture of both groups showed that cultures originating from the trapped cells senesced after approximately 15 days while the non-trapped keratinocytes grew for up to 40 days. Manufacture of an epidermis/dermal device (artificial skin) showed that non-trapped cells formed a significantly thicker epithelial layer than the trapped cells, demonstrating the enhanced regenerative capability of the smaller diameter, alpha 6 integrin(bri)CD71(dim) cells separated by GACS.
    Conclusion: These results indicate that GACS is simple and useful technique to enrich for epidermal progenitor/stem cell populations, and is more efficient when used on cells in culture. (C) 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.

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  • Pharmacological retention of oral mucosa progenitor/stem cells 査読

    K. Izumi, K. Inoki, Y. Fujimori, C. L. Marcelo, S. E. Feinberg

    Journal of Dental Research   88 ( 12 )   1113 - 1118   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Oral mucosa progenitor/stem cells reside as a small-sized cell population that eventually differentiates concurrently with an increase in cell size. Activation of the mammalian target of rapamycin (mTOR) leads to an increase in cell size. We hypothesized that rapamycin, a specific inhibitor of mTOR, will maintain primary human oral keratinocytes as a small-sized, undifferentiated cell population capable of retaining their proliferative capacity. Primary, rapamycin-treated (2 nM, 20 nM) oral keratinocytes showed a diminished cell size that correlated with a higher clonogenicity, a longer-term proliferative potential, and a slower cycling cell population concurrent with decreased expression of a differentiation marker when compared with untreated cells. Only the 2-nM rapamycin-treated oral keratinocytes maintained their ability to regenerate oral mucosa in vitro after 15 weeks of culture. Rapamycin, a Food and Drug Administration-approved drug, may have applicability for use in creating a highly proliferative cell population for use in regenerative medicine.

    DOI: 10.1177/0022034509350559

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  • Constitutive Release of Cytokines by Human Oral Keratinocytes in an Organotypic Culture 査読

    Qin Xu, Kenji Izumi, Takayoshi Tobita, Yoshitaka Nakanishi, Stephen E. Feinberg

    JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY   67 ( 6 )   1256 - 1264   2009年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:W B SAUNDERS CO-ELSEVIER INC  

    Purpose: The Food and Drug Administration requires an accurate determination of the dose and potency of tissue-engineered or combination products as is required for drugs. This needs to be done as a rapid, quantitative, and noninvasive measurement of biologic function/activity in a way so as not to perturb the tissue-engineered product being developed. The aim of this study was to correlate constitutive release of cytokine(s) from unstimulated cells, at different stages of development, within a 3-dimensional (3D) organotypic ex vivo produced oral mucosa equivalent (EVPOME) to be used for intraoral grafting, with oral keratinocyte cell viability of the EVPOME.
    Materials and Methods: Tissue culture medium was assayed with an enzyme-linked immunosorbent assay from monolayer culture of oral keratinocytes and a 3D EVPOME to determine the constitutive release of interleukin (IL) 1 alpha, IL-6, IL-8, and vascular endothelial growth factor (VEGF). VEGF messenger ribonucleic acid expression by oral keratinocytes within the 3D EVPOME was detected by in situ hybridization at days 4, 7, and 11. The number of viable oral keratinocytes within the EVPOME was extrapolated from VEGF release by use of a modified MTT assay.
    Results: Both VEGF release level and the number of viable cells in the monolayer cultures and 3D EVPOME as measured by MTT assay significantly increased in a time-dependent manner (P &lt; .001, r = 0.743).
    Conclusion: These results suggest that the increasing detectable levels of VEGF associated with the increasing number of Viable cells in the EVPOME may provide a useful noninvasive/nondestructive means of assessing both cellular viability (dose) and biologic function/activity (potency) of a combination cell-based device such as the EVPOME. (C) 2009 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 67:1256-1264, 2009

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  • The expression and production of vascular endothelial growth factor in oral mucosa equivalents 査読

    Y. Nakanishi, K. Izumi, M. Yoshizawa, C. Saito, Y. Kawano, T. Maeda

    INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY   36 ( 10 )   928 - 933   2007年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CHURCHILL LIVINGSTONE  

    Angiogenesis is anticipated during wound healing. Vascular endothelial growth factor (VEGF) is a potent direct angiogenic factor that stimulates endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Ex vivo produced oral mucosa equivalent (EVPOME) grafts have been reported to promote revascularization in the underlying tissue after grafting. The aim of this study was to evaluating the following: VEGF mRNA and its protein expression in EVPOME grafts, the protein levels in conditioned media produced by EVPOMEs, and the ability of VEGF to stimulate the growth of microvascular endothelial cells. VEGF mRNA expression and immunoreaction were found in basal and suprabasal layers. VEGF secreted by EVPOME was detected throughout the period of manufacture of the grafts, and became elevated for the first week at an air-liquid interface. Both EVPOME-conditioned media and a medium containing 10 ng/mL recombinant human VEGF induced endothelial cell proliferation, while neutralization of VEGF by an antibody blocked cell growth. These results suggest that VEGF secreted by EVPOME grafts might assist initial vascularization after grafting, which is critical to subsequent graft survival.

    DOI: 10.1016/j.ijom.2007.06.013

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  • 癌治療への再生医療の応用 培養複合口腔粘膜(EVPOME)の口腔癌治療への応用

    芳澤 享子, 泉 健次, 飯田 明彦, 鈴木 一郎, 齊藤 力, 高木 律男, 寺師 浩人, 横尾 聡, 古森 孝英, 津野 宏彰, 古田 勲

    再生医療   6 ( 2 )   203 - 208   2007年5月

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    記述言語:日本語   出版者・発行元:(株)メディカルレビュー社  

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  • Sensing metabolic activity in tissue engineered constructs 査読

    Malavika Chandra, Robert H. Wilson, Wen-Liang Lo, Karthik Vishwanath, Kenji Izumi, Stephen E. Feinberg, Mary-Ann Mycek

    DIAGNOSTIC OPTICAL SPECTROSCOPY IN BIOMEDICINE IV   6628   2007年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:SPIE-INT SOC OPTICAL ENGINEERING  

    Tissue engineered constructs can be employed to graft wounds or replace diseased tissue. Non-invasive methods are required to assess cellular viability in these constructs both pre- and post- implantation into patients. In this study, Monte Carlo simulations and fluorescence experiments were executed on ex vivo produced oral mucosa equivalent (EVPOME) constructs to investigate the fluorescence emitted at 355 nm excitation from these constructs. Both simulations and experiments indicated the need to investigate alternative excitation wavelengths in order to increase the cellular fluorescence from these constructs, while decreasing contributions from extra-cellular fluorophores.

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  • 口腔癌の新しい治療戦略 培養複合口腔粘膜の臨床応用

    寺師 浩人, 泉 健次, 田原 真也, 横尾 聡, 古森 孝英, 芳澤 享子, 鈴木 一郎, 齋藤 力, 飯田 明彦, 高木 律男, 津野 宏彰, 古田 勲

    頭頸部癌   32 ( 3 )   281 - 285   2006年10月

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    記述言語:日本語   出版者・発行元:(一社)日本頭頸部癌学会  

    われわれの開発した培養複合上皮(皮膚、口腔粘膜)は、動物由来蛋白や3T3マウスfeeder layerを使用せずに完全無血清培地でkeratinocyteを培養した移植材料である。培養細胞を無細胞真皮上に播種して作製される培養複合上皮は、文部科学省高度先進医療開発経費Bの支援のもと、口腔粘膜上皮に関して新潟大学、富山大学、神戸大学の三施設合同で臨床応用され、平成14年〜16年の三年間で106症例を数えた。その内52症例が口腔癌もしくはin situであり、結果として90症例で成功し、何らかの理由により16症例で不成功となった。この方法の利点は、異種移植と定義されないこと、理論上将来のプリオン病への危惧がほぼ皆無であること、把持縫合可能な操作性を有すことである。欠点として、無細胞であるが他人の真皮をscaffoldとして使用すること、作製に約一ヵ月を要することである。(著者抄録)

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  • High glucose inhibits human epidermal keratinocyte proliferation for cellular studies on diabetes mellitus 査読

    Hiroto Terashi, Kenji Izumi, Mustafa Deveci, Lenore M. Rhodes, Cynthia L. Marcelo

    International Wound Journal   2 ( 4 )   298 - 326   2005年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In order to more clarify the delayed wound healing in diabetes mellitus, we cultured the human epidermal keratinocytes in both 6 mM (control group) and 12 mM glucose (high-glucose group) of 'complete' MCDB 153 medium. Hyperglycaemia slowed the rate of their proliferation and inhibited their DNA synthesis and the production of total proteins. By 1 month after primary seeding in high-glucose group, the cells ceased their proliferation, whereas the cells in control group grew for more than 40 days. Mean population doublings in high-glucose group was 5.27 (vs. 7.25 in control, P = 0.001), and mean population doubling time during 1 month in high glucose group was 5.43 days (vs. 3.65 days in control, P = 0.02). They indicate that prolonged exposure to high glucose decreases the replicative life span of human epidermal keratinocytes in vitro. Furthermore, analysis of fatty acid contents in membrane phospholipids with thin-layer and gas chromatography showed no difference between the cultured keratinocytes in both conditions. Immunocytochemical staining of glucose transporter 1 shows that 28.1% of cells in high-glucose group were almost twice positive of those in control group (13.2%, P = 0.008). The mechanism of the ill effects of high glucose on epidermal keratinocytes is not so far clear, but it indicates the possibility of any direct effect of hyperglycaemia on glucose metabolism without changing lipid metabolism on cell membrane. The high-glucose group presented in this report can be available as an in vitro valuable study model of skin epidermal condition on diabetes mellitus. © Blackwell Publishing Ltd and Medicalhelplines.com Inc 2005.

    DOI: 10.1111/j.1742-4801.2005.00148.x

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  • Development of a tissue-engineered human oral mucosa: From the bench to the bed side 査読

    K Izumi, JH Song, SE Feinberg

    CELLS TISSUES ORGANS   176 ( 1-3 )   134 - 152   2004年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    The main objective of this publication is to make the reader aware of the complexity and steps that are necessary to make a Food and Drug Administration (FDA)-approved laboratory produced cell-based device, for use in clinical trials for reconstructive surgery. Most tissue-engineered cell-based devices are considered as 'human somatic cell therapy' and fall under the auspices of the Center of Biologic Evaluation and Research (CBER) and are considered a combination product by the FDA. We have illustrated the algorithm that is necessary to follow an Independent New Drug (IND) application by using our ex vivo produced oral mucosa equivalents (EVPOME), a tissue-engineered oral mucosa, as an example of a cell-based device that needs FDA approval prior to clinical application. By illustrating the experimental approach and presenting resulting data we attempt to explain each step that we address along the way. Copyright (C) 2004 S. Karger AG, Basel.

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  • Malignant fibrous histiocytoma arising in abutting soft tissue after resection of a benign fibrous histiocytoma of the mandible 査読

    Kenji Izumi, Jun Cheng, Takeyasu Maeda, Takashi Saku

    Oral Oncology Extra   40 ( 2 )   24 - 28   2004年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    We present a unique case of malignant fibrous histiocytoma (MFH) developing around a masseter muscle close to the surgical margin from which a benign fibrous histiocytoma (BFH) of the mandible was excised 3 years and 10 months earlier. In the interval between the surgical removal of the original BFH and the MFH, several surgical procedures were performed in the related region. Clinical and histopathological evidence showed that this case represented two separate lesions rather than a single disease process. The clinical history suggests that the chronic repair process might have contributed to the tumorigenic factor of a MFH. © 2004 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.ooe.2003.12.001

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  • Intraoral grafting of an ex vivo produced oral mucosa equivalent: a preliminary report 査読

    K Izumi, SE Feinberg, A Iida, M Yoshizawa

    INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY   32 ( 2 )   188 - 197   2003年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CHURCHILL LIVINGSTONE  

    The objective of this study was to assess the efficacy of the use of an ex vivo produced oral mucosa equivalent (EVPOME) for intraoral grafting procedures. Autogenous keratinocytes were harvested from a punch biopsy 4 weeks phor to surgery. placed in a serum-free culture system and seeded onto a human cadaveric dermal equivalent, AlloDerm (R). Thirty patients with either a premalignant or cancerous lesion were triaged into two groups, depending on the stage of disease: Group 1: EVPOME or Group 2: AlloDerm (R), control without an epithelial layer. Clinically, EVPOME grafts were easy to handle and showed excellent compliance on grafting. Both, EVPOME and AlloDerm (R) grafts, showed a 100% take rate, At 6 days post-grafting, the EVPOME clinically showed changes indicating vascular ingrowth and had cytologic evidence of the persistence of grafted cultured keratinocytes on the surface. The EVPOME grafts had enhanced maturation of the underlying submucosal layer associated with rapid epithelial coverage when compared to the AlloDerm (R) grafts at biopsies taken at 28 days post-grafting. In summary, EVPOME appears to be an acceptable oral mucosal substitute for human intraoral grafting procedures and results in a more favorable wound healing response than AlloDerm (R) alone.

    DOI: 10.1054/ijom.2002.0365

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  • Evaluation of transplanted tissue-engineered oral mucosa equivalents in severe combined immunodeficient mice 査読

    K Izumi, SE Feinberg, H Terashi, CL Marcelo

    TISSUE ENGINEERING   9 ( 1 )   163 - 174   2003年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MARY ANN LIEBERT INC PUBL  

    The aim of this study was to determine the optimal stage of development at which transplant human ex vivo-produced oral mucosa equivalents (EVPOMEs) in vivo. EVPOMEs were generated in a serum-free culture system, without the use of an irradiated xenogeneic feeder layer, by seeding human oral keratinocytes onto a human cadaveric dermal equivalent, AlloDerm. EVPOMEs were cultured for 4 days submerged and then for 7 or 14 days at an air-liquid interface to initiate stratification before transplantation into SCID mice. AlloDerm, without epithelium, was used as a control. Mice were killed on days 3, 10, and 21 posttransplantation. Epithelium of the transplanted EVPOMEs was evaluated with the differentiation marker keratin 10/13. Dermal microvessel ingrowth was determined by immunohistochemistry with a mouse vascular marker, lectin binding from Triticum vulgaris. The presence and stratification of the epithelium were correlated with revascularization of the underlying dermis. The microvessel density of AlloDerm without epithelium was less than that of EVPOMEs with an epithelial layer. Microvessel density of the dermis varied directly with the degree of epithelial stratification of the EVPOMEs. The EVPOMEs cultured at an air-liquid interface for 7 days had the optimal balance of neoangiogenesis and epithelial differentiation necessary for in vivo grafting.

    DOI: 10.1089/107632703762687645

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  • 下顎骨再建にトランスポート骨延長法を応用した2例

    小林 正治, 泉 健次, 本間 克彦, 他

    新潟歯学会雑誌   32 ( 2 )   87 - 91   2002年12月

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    記述言語:日本語   出版者・発行元:新潟大学  

    Distraction osteogenesis is a method of increasing bone length, and is now being used in the craniofacial region. The authors report two cases in which segmental mandibular defects were reconstructed by transport distraction osteogenesis and bone transplantation. The patients were a 67-year-old man and a 54-year-old man with squamous cell carcinoma of the lower gingiva and their mandible had been resected from the first molar on the right side to the mandibular angle region on the left side. In the first patient, transport distraction osteogenesis in the mandible was performed at the both sides and low-intensity pulsed ultrasound was used to accelerate bone healing. In the second patient, transport distraction osteogenesis was performed at the right side. Although bone transplants were needed for complete continuity in both cases, segmental mandibular defects were decreased by the regenerated bone. Successful new bone formations in the lengthened areas were recognized by radiographic observation, especially in the old patient used low-intensity pulsed ultrasound. Low-intensity pulsed ultrasound may be effective measures to accelerate bone healing after distraction osteogenesis.骨延長法は、骨そのものを牽引延長することにより骨の増生をはかる方法で、近年顎顔面領域においても広く応用されるようになってきた。今回われわれは、下顎骨欠損に対してトランスポート骨延長と骨移植を組み合わせた下顎骨再建を2症例に施行したので報告する。患者は、67歳と54歳の男性で、ともに下顎歯肉癌の診断にて右側第一大臼歯から左側下顎角部の下顎骨区域切除を施行した。症例1では、両側において骨トランスポート法による下顎骨延長を行い、低出力超音波パルス照射を応用して骨形成の促進を囲った。症例2では、右側において骨トランスポート法による下顎骨延長を行った。両症例とも骨の延長に伴い欠損範囲を縮小し、骨移植を容易にすることができた。特に、低出力超音波パルス照射を応用した症例1では、高齢にも関わらず良好な骨形成が認められ、骨延長における骨形成促進に低出力超音波パルス照射は有用ではないかと思われた。

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  • 顎裂部骨移植後の咬合形成に関する臨床的検討

    泉 健次, 小林正治, 本間克彦, 新垣 晋, 齊藤 力, 寺田員人, 石井一裕, 森田修一, 野村章子

    日口蓋誌   2002年4月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.11224/cleftpalate1976.27.1_58

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  • Skin and oral mucosal substitutes 査読

    Kenji Izumi, Stephen E. Feinberg

    Oral and Maxillofacial Surgery Clinics of North America   14 ( 1 )   61 - 71   2002年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To date, successfully developed EVPOMEs in a serum-free culture system without a feeder layer are the most acceptable and promising oral mucosal substitutes for human intraoral grafting because of minimal risk of foreign contaminants, easy handling and stitching, subsequent rapid revascularization into dermal component after transplantation, and contribution to favorable open wound closure without functional compromise, although several types of oral mucosal substitutes described in this article have been used in patients.

    DOI: 10.1016/S1042-3699(02)00010-9

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  • Human stratified squamous epithelia differ in cellular fatty acid composition 査読

    H Terashi, K Izumi, LM Rhodes, CL Marcelo

    JOURNAL OF DERMATOLOGICAL SCIENCE   24 ( 1 )   14 - 24   2000年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    The phospholipid component of the cellular membrane is crucial to the structure and function of cells. Basal cells from three epithelial tissues, adult human skin epidermis, oral mucosa, and hair follicles, grow rapidly in serum- and lipid-free medium. Analysis of phospholipid extracts from the above three types of stratified squamous epithelium in both in vivo and in vitro was done to relate fatty acid cell composition to cell function. The fatty acid composition of hair follicles in vivo was analyzed in plucked scalp hairs, and those of skin epidermis and oral mucosa in vivo were analyzed after separating the tissue into suprabasal and basal layers. The fatty acid composition of the in vivo cells from hair follicles shows a partial essential fatty acid (EFA)-deficient state. There was no significant difference between the skill epidermis and the oral mucosa in the fatty acid composition of the in vivo cells from each basal layer. However, in the suprabasal layers, the percent of linoleic acid (18:2) from the skin epidermis was higher than that from the oral mucosa. This study shows that total fatty acid composition in cell membranes of stratified squamous epithelium varies with their keratinization pattern. When cultured, the three types of rapidly growing keratinocytes showed the same essential fatty acid deficient pattern in the membrane phospholipids. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

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  • 自家移植歯の歯根膜治癒と受容側因子の関係

    濱本 宜興, 濱本 尚希, 泉 健次, 小林 正治, 中島 民雄, 毛利 環, 花田 晃治

    日本口腔外科学会雑誌   43 ( 10 )   733 - 738   1997年10月

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    記述言語:日本語   出版者・発行元:社団法人 日本口腔外科学会  

    The relationship between recipient condition and the period required for periodontal healing of autotransplanted teeth was studied. The period for periodontal healing was evaluated on the basis of regeneration of the periodontal space and lamina dura on dental radiographs. Age, sex, site of transplantation, period after tooth extraction at the recipient site, and the timing of occlusal function were the recipient factors examined. The mean periods required for regeneration of the periodontal space and lamina dura were 3.7 months and 7.6 months, respectively. Regeneration of the periodontal space was not influenced by any recipient factor, whereas regeneration of lamina dura was associated with the period after tooth extraction, site of transplantation, and timing of occlusal function. It was suggested that regeneration of the lamina dura may not totally depend on that of the periodontal space.

    DOI: 10.5794/jjoms.43.733

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  • 顎変形症手術における術中合併症について

    鈴木君弘, 泉健次, 本間克彦, 小林正治, 中島民雄

    日顎変形誌   7 ( 2 )   141 - 146   1997年10月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:特定非営利活動法人 日本顎変形症学会  

    Unfavorable fractures and blood vessel injuries encountered during 196 operations on 185 patients who underwent surgical correction of jaw deformities were studied to analyze the causes. There were 12 unfavorable fractures (6.1%) and 9 blood vessel injuries (4.6%). All complications occurred in two-jaw surgery and sagittal splitting ramus osteotomy (SSRO). Blood loss and operation time were significantly higher in cases with complications than in those without complication in SSRO.<BR>SSRO. With the exception of one case, all fractures were encountered during SSRO. Most of them were minor fractures occurring along the osteotomy line, but relatively large segments of the proximal segment were fractured in 3 cases, 2 of which were not repositioned. Blood vessel injury of the descending palatine artery was encountered in 2 cases of Le Fort I osteotomy. In SSRO, injuries of the inferior alveolar artery, facial artery, and unidentified artery occurred in 3, 1, and 3 cases, respectively. Ligation of the arteries was required to stop bleeding in 2 cases, whereas in the other cases, bleeding was controlled by packing of gauze or oxidized regenerated cellulose.<BR>Insufficient bony cut, forcible splitting, and careless handling of surgical instruments were the main causes of the complications. It is thought that the complications can be avoided by precise analysis of structures at the operation site by computed tomography, sufficient bony cut, and careful handling of surgical instruments.

    DOI: 10.5927/jjjd1991.7.141

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  • 片側顎関節強直症の1治験例

    小林 正治, 本間 克彦, 泉 健次, 鈴木 君弘, 中島 民雄, 花田 晃治

    日本顎変形症学会雑誌   7 ( 1 )   57 - 62   1997年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本顎変形症学会  

    A case of unilateral ankylosis of the temporomandibular joint, which caused micrognathia with facial asymmetry, was reported. A 3-year-old female visited our department because of facial asymmetry She was placed on conservative therapy using a functional appliance to improve facial asymmetry and occlusal relationship. Because of mandibular trismus, condylectomy was performed at the age of 13 years 10 months. Interincisal distance increased to 38mm, but micrognathia with facial asymmetry remained. Therefore, Le Fort I osteotomy combined with extraoral vertical ramus osteotomy on the affected side and sagittal split ramus osteotomy on the opposite side was performed at the age of 16 years 10 months. In addition, augmentation genioplasty with a porous hydroxyapatite block was performed at the age of 17 years 6 months. Four years later, the patient was satisfied with recovery of jaw opening and improvement in facial appearance.

    DOI: 10.5927/jjjd1991.7.57

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  • 顎関節症患者の臨床統計的観察

    岡田朋子, 濱本宣興, 小林正治, 本間克彦, 泉健次, 高田佳之, 中島民雄

    日顎誌   1996年9月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • 自家移植歯の根管充填状態と歯根膜治癒との関係

    濱本 宜興, 小林 豊, 濱本 尚希, 泉 健次, 小林 正治, 中島 民雄, 毛利 環, 花田 晃治

    日本口腔外科学会雑誌   42 ( 7 )   684 - 689   1996年7月

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    記述言語:日本語   出版者・発行元:社団法人 日本口腔外科学会  

    The relationship between root canal filling and the periodontal healing of autotransplantated teeth was studied clinically and radiographically in 21 autotransplanted teeth with complete root formation that were followed up for more than 6 months. The teeth were classified into three types according to the status of root canal filling immediately after transplantation: type A) teeth with root canal (s) wide enough to be detected on radiographs and root canal filling (s) to the apex (es); type B) teeth having incomplete root canal filling (s) because of narrow root canal (s); and type C) teeth having root canal (s) detectable on radiographs but with incomplete filling (s). The periodontal healing was examined more than 6 months postoperatively. Ten teeth classified as type A had no clinical symptoms and showed regeneration of the periodontium on radiographs. Eight teeth classified as type B had no clinical symptoms but the periodontal spaces were indistinct in 5 teeth. No clinical symptoms were associated with 2 of 3 teeth classified as type C, but the periodontal spaces were not clearly seen in the apical regions. The presence of apical lesions and inflammatory resorption of the root was observed on radiographs of the other tooth. These findings suggest that periodontal healing is associated with the width of the root canal and the status of root canal filling.

    DOI: 10.5794/jjoms.42.684

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  • Tooth autotransplantation and periodontal damage 査読

    Y Hamamoto, K Izumi, T Kobayashi, T Nakajima

    3RD ASIAN CONGRESS ON ORAL AND MAXILLOFACIAL SURGERY   561 - 565   1996年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:MONDUZZI EDITORE  

    The effect of damage to the periodontium on the outcome of tooth transplantation was studied by examining the relationship of macroscopic condition of the root surface to the clinical course in 27 human autotransplanted teeth. The macroscopic conditions were classified into the areas with A) remnants of periodontal tissue, B) cementum exposure, and C) cementum loss. The macroscopic evaluation was confirmed to be well correlated with the histological findings or 10 healthy teeth. Most of the root surfaces of autotransplanted teeth consisted of the areas with cementum exposure and attachment of periodontal tissue in varying proportions. These teeth showed normal periodontal healing both clinically and radiographically. Replacement resorption in the area of cementum loss was observed in one tooth. The results were consistent with those of previous reports which showed that cementum is important for normal periodontal healing.

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  • Nerve terminals immunoreactive to neurofilament protein (NFP) in the periodontal ligament of rat molars. 査読

    N. Kamasaki, T. Maeda, K. Izumi, Y. Kozawa

    Jap. J Oral Biol.   34 ( 2 )   239 - 243   1992年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2330/joralbiosci1965.34.239

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  • 実験的ハムスター舌癌組織における神経線維の動態:抗NFP、S-100蛋白抗体を用いた免疫組織学的研究 査読

    泉 健次, 前田健康

    口外誌   38 ( 6 )   896 - 904   1992年

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書籍等出版物

  • 口腔粘膜組織再生医療:歯科再生医学

    泉 健次( 担当: 共著 ,  範囲: 第4章、第5項)

    医歯薬出版  2019年4月 

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  • スタンダード歯科理工学第7版

    泉 健次( 担当: 共著 ,  範囲: 第4章 生体材料の安全性と適合性)

    学建書院  2019年3月 

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  • Tissue engineering:Operative oral and maxillofacial surgery

    Smith MH, Izumi K, Feinberg SE( 担当: 共著 ,  範囲: Chapter 2-11)

    CRC Press, Abingdon, UK  2016年 

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  • 口腔の軟組織 臨床的考察:口腔組織・発生学

    泉 健次( 担当: 共著 ,  範囲: 第9章)

    医歯薬出版  2015年 

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  • 培養口腔粘膜作成技術と製品評価, 『再生医療事業の課題解決のための手引書』

    加藤寛子, 泉 健次( 担当: 共著 ,  範囲: 第6部2章2節5項)

    技術情報協会  2013年 

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  • Skin Stem Cells

    Izumi K, Marcelo CM, Feinberg SE( 担当: 共著 ,  範囲: Chapter 23)

    Humana Press  2013年 

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  • 口腔粘膜の再生医療:再生医療叢書

    泉 健次( 担当: 共著 ,  範囲: 第8巻 歯学系)

    朝倉書店  2012年 

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  • Tissue engineering. Current therapy in oral and maxillofacial surgery

    Smith MH, Izumi K, Feinberg SE( 担当: 共著 ,  範囲: Chapter 9)

    Elsevier Saunders  2012年 

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  • Skin and oral mucosal substitutes

    W.B. SAUNDERS.  2001年 

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  • Electron microscopic atlas of oral disease

    Nagasue Shoten  1998年 

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  • Athas of electron microscope of oral disease

    1996年 

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MISC

  • 画像解析による口腔ケラチノサイトの細胞増殖能の非侵襲的評価

    干川絵美, 干川絵美, 佐藤大祐, 鈴木絢子, 羽賀健太, 羽賀健太, 多部田康一, 泉健次

    新潟歯学会雑誌   50 ( 2 )   2020年

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  • マイクロパターン化した魚うろこコラーゲン足場材を用いた培養口腔粘膜の開発

    鈴木絢子, 鈴木絢子, 加藤寛子, 干川絵美, 羽賀健太, 塩見晶, 上野山敦士, 兒玉泰洋, 河上貴宏, 三輪慶人, 桑江博之, 塩澤茉由子, 水野潤, 齋藤一誠, 早崎治明, 泉健次

    日本再生医療学会総会(Web)   18th   2019年

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  • 細胞品質管理に向けた画像解析による口腔ケラチノサイトの非侵襲的,定量的運動能評価の試み

    干川絵美, 干川絵美, 木森義隆, 佐藤大祐, 加藤寛子, 鈴木絢子, 羽賀健太, 難波大輔, 多部田康一, 泉健次

    日本再生医療学会総会(Web)   18th   2019年

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  • 魚のうろこコラーゲンを足場に利用した培養口腔粘膜の開発

    鈴木絢子, 鈴木絢子, 加藤寛子, 加藤寛子, 干川絵美, 塩見晶, 上野山敦士, 河上貴宏, 兒玉泰洋, 齋藤一誠, 早崎治明, 泉健次

    日本再生医療学会総会(Web)   17th   2018年

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  • ROCK阻害剤の骨代謝および矯正学的歯の移動への影響

    中田 樹里, 柿原 嘉人, 秋葉 陽介, 江口 香里, 丹原 惇, 大倉 麻里子, 加藤 寛子, 泉 健次, 佐伯 万騎男, 齋藤 功

    新潟歯学会雑誌   47 ( 2 )   120 - 120   2017年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • うろこコラーゲンを使用した培養口腔粘膜の開発

    鈴木絢子, 鈴木絢子, 加藤寛子, 加藤寛子, 干川絵美, 塩見晶, 河上貴宏, 兒玉泰洋, 齋藤一誠, 早崎治明, 泉健次

    日本バイオマテリアル学会大会予稿集(Web)   39th   97 (WEB ONLY)   2017年11月

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    記述言語:日本語  

    J-GLOBAL

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  • ホーム、スイートホーム -口腔粘膜ティッシュエンジニアリングの展望ー 招待 査読

    泉 健次

    新潟歯学会雑誌   47 ( 1 )   1 - 10   2017年6月

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  • コラーゲンゲル内で3次元培養したヒト口腔粘膜線維芽細胞の低酸素応答

    原 夕子, 加藤 寛子, 塩見 晶, 高木 律男, 泉 健次

    新潟歯学会雑誌   46 ( 2 )   114 - 114   2016年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • ラット間葉系細胞の多面的骨誘導能に対する低酸素処理の効果に関する検討

    齋藤 直朗, 泉 健次, 秋葉 陽介, 加藤 寛子, 原 夕子, 小島 拓, 芳澤 享子, 小林 正治, 大峡 淳, 前田 健康

    新潟歯学会雑誌   45 ( 2 )   106 - 106   2015年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

    J-GLOBAL

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  • 低酸素環境が培養ヒト正常口腔粘膜上皮細胞に及ぼす影響

    加藤寛子, 泉健次, 原夕子, 塩見晶, 上野山敦士, 前田健康

    組織培養研究   34 ( 1 )   34   2015年3月

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    記述言語:日本語  

    J-GLOBAL

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  • C配糖体が口腔粘膜上皮角化細胞と線維芽細胞から成る3次元口腔粘膜モデルに及ぼす影響の検討

    上野山敦士, 泉健次, 塩見晶, 齋藤直朗, 原夕子, 齋藤太郎, 大貫尚志, 加藤寛子, 安島久雄, 高木律男, 前田健康

    新潟歯学会雑誌   44 ( 2 )   127   2014年12月

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  • Hypoxia Induces Undifferentiated Phenotype of Oral Keratinocytes In Vitro

    H. Kato, K. Izumi, A. Shiomi, A. Uenoyama, S. Kuo, S. Feinberg, T. Maeda

    TISSUE ENGINEERING PART A   20   S17 - S17   2014年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:MARY ANN LIEBERT, INC  

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  • C配糖体が培養口腔粘膜上皮角化細胞に及ぼす影響の検討

    上野山敦士, 泉健次, 塩見晶, 齋藤直朗, 齋藤太郎, 大貫尚志, 加藤寛子, 寺田(中石)典子, 河野芳朗, 野澤(井上, 佳世子, 高木律男, 前田健康

    再生医療   13   234   2014年1月

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  • 培養口腔粘膜上皮細胞における細胞分化とTASCCに関する検討

    泉健次, 加藤寛子, 大貫尚志, 齋藤太郎, 塩見晶, 上野山敦士, 寺田典子, 河野芳朗, 野澤(井上, 佳世子, 前田健康

    日本解剖学会総会・全国学術集会講演プログラム・抄録集   118th   144   2013年

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  • 3D口腔粘膜モデルを用いたビスフォスフォネート製剤が創閉鎖に及ぼす影響の組織学的・免疫組織化学的検討

    齋藤太郎, 泉健次, 上野山敦士, 塩見晶, 大貫尚志, 加藤寛子, 寺田典子, 河野芳朗, 野澤(井上, 佳世子, 高木律男, 前田健康

    新潟歯学会雑誌   42 ( 2 )   142 - 143   2012年12月

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  • Histological and immunohistological analyses of epithelial regeneration and wound healing affected by Bisphosphonates (BPs) using a living oral mucosa equivalent model

    T. Saito, K. Izumi, A. Shiomi, H. Ohnuki, H. Kato, M. Terada, Y. Kawano, K. Nozawa-Inoue, R. Takagi, T. Maeda

    JOURNAL OF INVESTIGATIVE DERMATOLOGY   132   S135 - S135   2012年5月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:NATURE PUBLISHING GROUP  

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  • ゾレドロン酸がヒト口腔粘膜上皮細胞に及ぼす影響に関する研究

    大貫尚志, 泉健次, 加藤寛子, 齋藤太郎, 寺田典子, 河野芳郎, 野澤(井上, 佳世子, 高木律男, 前田健康

    新潟歯学会雑誌   41 ( 2 )   119 - 120   2011年12月

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  • ALDH活性の薬理学的操作が口腔粘膜角化細胞形質と上皮組織に及ぼす影響の検討

    加藤寛子, 泉健次, 大貫尚志, 齋藤太郎, 寺田典子, 河野芳朗, 野澤(井上, 佳世子, 齊藤力, 前田健康

    新潟歯学会雑誌   41 ( 2 )   128   2011年12月

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  • ラット顎関節滑膜におけるデスミン陽性B型表層細胞

    野澤(井上, 佳世子, 鈴木晶子, 真柄仁, 河野芳朗, 寺田典子, 泉健次, 前田健康

    J Oral Biosci   53 ( Supplement )   162   2011年9月

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  • キトサンおよびウロココラーゲンを足場とした培養複合口腔粘膜の組織学的検討

    寺田典子, 泉健次, 加藤寛子, 大貫尚志, 齋藤太郎, 前田健康, 山本麻里絵, 生駒俊之, 田中順三

    再生医療   10   170   2011年2月

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  • 顎関節関節円板の発達におけるネスチンとGFAPの局在変化

    鈴木晶子, 野澤(井上, 佳世子, 真柄仁, 河野芳朗, 寺田典子, 泉健次, 前田健康

    解剖学雑誌   85 ( Supplement )   133   2010年3月

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    記述言語:日本語  

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  • 歯胚中間層における67kD laminin receptor(67LR)の発現:星状網における血管網形成との相関

    河野芳朗, 木下(河野)承子, 鈴木晶子, 野澤(井上, 佳世子, 泉健次, 前田健康

    J Oral Biosci   51 ( Supplement )   73   2009年8月

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  • 口腔粘膜の再建における培養複合口腔粘膜(EVPOME)の応用

    芳澤 享子, 泉 健次, 飯田 明彦, 小山 貴寛, 中西 義崇, 鈴木 一郎, 齊藤 力, 高木 律男

    新潟県医師会報   ( 702 )   8 - 10   2008年9月

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    記述言語:日本語   出版者・発行元:新潟県医師会  

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  • 培養複合口腔粘膜による口腔粘膜再建の臨床的評価

    芳澤 享子, 泉 健次, 飯田 明彦, 鈴木 一郎, 齊藤 力, 高木 律男, 寺師 浩人, 横尾 聡, 古森 孝英, 津野 宏彰, 古田 勲

    日本口腔外科学会雑誌   50 ( 13 )   953 - 954   2004年12月

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    記述言語:日本語   出版者・発行元:(公社)日本口腔外科学会  

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  • 口腔前庭拡張術(付着歯肉形成術)に培養複合口腔粘膜を使用した4例の臨床的検討

    飯田 明彦, 泉 健次, 高木 律男, 芳澤 享子, 鈴木 一郎, 齊藤 力

    日本口腔粘膜学会雑誌   8 ( 2 )   99 - 100   2002年12月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔内科学会  

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  • 智歯の移植に関する臨床的検討

    川上 美貴, 芳澤 享子, 小林 正治, 泉 健次, 齋藤 力, 安島 久雄, 小野 和宏, 高木 律男

    日本口腔科学会雑誌   51 ( 6 )   514 - 515   2002年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本口腔科学会  

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  • A progressive mandibular radio lucency

    ORAL SURG ORAL MED ORAL PATHOL ORAL RADIOL ENDOD   2001年

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  • Expression of glucose transporter 1 in epithelial layer of human oral mucosa equivalent

    Japanese Journal of Oral and Maxillofacial Surgery   47 ( 5 )   289 - 292   2001年

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  • ヒト培養複合口腔粘膜上皮層におけるGlucose transporter 1(GLUT1)発現

    泉 健次, 寺師 浩人, 芳澤 享子

    日本口腔外科学会雑誌   47 ( 5 )   289 - 292   2001年

  • Development and characterization of a tissue-engineered human oral mucosa equivalent produced in a serum-free culture system

    K Izumi, H Terashi, CL Marcelo, SE Feinberg

    JOURNAL OF DENTAL RESEARCH   79 ( 3 )   798 - 805   2000年3月

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    記述言語:英語   出版者・発行元:AMER ASSOC DENTAL RESEARCH  

    A problem maxillofacial surgeons face is a lack of sufficient autogenous oral mucosa for reconstruction of the oral cavity. Split-thickness or oral mucosa grafts require more than one surgical procedure and can result in donor site morbidity. Skin has disadvantages of adnexal structures and a different keratinization pattern than oral mucosa. In this study, we successfully assembled, ex vivo, a human oral mucosa equivalent, consisting of epidermal and dermal components, in a defined, essential-fatty-acid-deficient, serum-free culture medium without a feeder layer, that could be used for intra-oral grafting in humans. Autogenous oral keratinocytes were seeded onto a cadaveric dermis, AlloDerm(TM). The oral mucosa equivalent was cultured at an air-liquid interface for 2 wks. The resulting equivalent had a well-stratified parakeratinized epithelial layer similar to native oral keratinized mucosa. Expression of differentiation markers, filaggrin and cytokeratin 10/13, suggested a premature keratinized state. The presence of proliferation markers, proliferating cell nuclear antigen (PCNA) and Ki-67, suggested a state of hyperproliferation. Fatty acid composition of the equivalent was similar to that of in vitro cultured oral keratinocytes but differed from the that of in vivo native tissue, showing a lower content of 18:2 and 20:4, and a higher content of 16:1 and 18:1 fatty acids, respectively. The keratinocytes of the equivalent appeared to be in a more active and proliferative state than native keratinized mucosa. The dynamic nature of the cell population on the oral mucosa equivalent may be beneficial for intra-oral grafting procedures and for transfection of the keratinocytes.

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  • Clinical Application of oral mucosa equivalent-Intraoral grafting of human cultured oral mucosa composite-

    THE. QUINTESSENCE   20 ( 6 )   1057 - 1064   2000年

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  • -培養複合口腔粘膜の口腔内移植-培養粘膜の臨床応用

    クインテッセンス社   20 ( 6 )   1057 - 1064   2000年

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  • Polypoid mass of the gingiva

    K Izumi, T Nakajima, T Maeda, T Irie, M Murata, T Saku

    ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS   88 ( 2 )   117 - 121   1999年8月

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    記述言語:英語   出版者・発行元:MOSBY-YEAR BOOK INC  

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  • Ex vivo development of a composite human oral mucosal equivalent

    K Izumi, G Takacs, H Terashi, SE Feinberg

    JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY   57 ( 5 )   571 - 577   1999年5月

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    記述言語:英語   出版者・発行元:W B SAUNDERS CO  

    Purpose: The aim of this study was the ex vivo development of a composite oral mucosal equivalent composed of a continuous stratified layer of human oral keratinocytes grown on a cadaveric human dermal matrix in a defined medium without a feeder layer.
    Materials and Methods: Enzymatically dissociated human oral keratinocytes from keratinized oral mucosa were cultured, submerged in a serum-free, low-calcium (0.15 mmol/L) supplemented medium, and expanded through several passages. Once a sufficient population of keratinocytes was reached, they were seeded on 1-cm(2) pieces of AlloDerm (LifeCell Co, Woodlands, TX), an acellular nonimmunogenic cadaveric human dermis, at cell densities of 2.5 x 10(4), 5.0 x 10(4), 1.25 x 10(5), or 2.5 x 10(5). The oral keratinocyte-AlloDerm composites were cultured while submerged in a high-calcium (1.8 mmol/L) medium for 4 days. After 4 days, the composites were raised to an air-liquid interface. Samples of the composites were taken for histologic examination at 4, 11, and 18 days postseeding of the keratinocytes on the AlloDerm.
    Results: At day 4, only the seeded cell density of 2.5 x 10(5) cells/cm(2) formed a continuous monolayer on the AlloDerm. At day 11, a continuous stratified epithelium was seen, and at day 18 a well-differentiated, confluent parakeratotic epithelial layer was developed at cell densities of 5.0 x 10(4), 1.25 x 10(5), and 2.5 x 10(5) cells/cm(2).
    Conclusion: With the method used, it was possible to successfully develop an ex vivo composite oral mucosal equivalent that consisted of a stratified epidermis on a dermal matrix.

    DOI: 10.1016/S0278-2391(99)90077-0

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  • Development of oral mucosa equivalent ina serum-free culture system without afeedor layer

    Niigata Dental Journal   29 ( 2 )   187 - 188   1999年

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  • よりクリーンな培養複合口腔粘膜の開発

    新潟歯学会雑誌   29 ( 2 )   187 - 188   1999年

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  • Temporomandibular joint symptoms and disc displacement in patients with mandibular prognathism

    T. Kobayashi, K. Honma, K. Izumi, T. Hayashi, S. Shingaki, T. Nakajima

    British Journal of Oral and Maxillofacial Surgery   37 ( 6 )   455 - 458   1999年

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    記述言語:英語   出版者・発行元:Churchill Livingstone  

    Our objective was to find out the incidence of signs and symptoms of temporomandibular joint (TMJ) and disc displacement in patients with mandibular prognathism. Fifty-one patients were examined clinically and by axial computed tomography (CT). The incidence of TMJ signs and symptoms was 6/25 (24) in patients with simple mandibular prognathism and 12/26 (46) in patients with mandibular prognathism and asymmetry. No discs were displaced in patients with simple mandibular prognathism, but 15 (58) of the patients with mandibular prognathism and asymmetry had displaced discs. There was no association between signs and symptoms of TMJ and disc displacement. Patients with mild protrusion and severe asymmetry of the mandible had a high incidence of disc displacement, which interestingly was on the deviated side in 14 of the 15 patients affected. We conclude that skeletal morphology may have a role in the development of TMJ disorders but the mechanism is obscure.

    DOI: 10.1054/bjom.1999.0016

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  • Clinical study of surgically treated temporomandibular joint ankylosis

    Japanese Journal of Oral and Maxillofacial Surgery   45 ( 4 )   296 - 298   1999年

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  • 顎関節揺動術を施行した顎関節強直症の臨床的検討

    泉健次, 小林正治, 浜本宜興, 野村務, 横林敏夫, 中島民雄

    日本口腔外科学会雑誌   45 ( 4 )   296 - 298   1999年

  • Evaluation of ex vivo produced oral mucosa equivalent gratis transplanted to SCID mice

    Journal of Oral and Maxillofacial Surgery   57 ( 81Supple4 )   30   1999年

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  • Adenosquamous carcinoma of the tongue - Report of a case with histochemical, immunohistochemical, and ultrastructural study and review of the literature

    K Izumi, T Nakajima, T Maeda, J Cheng, T Saku

    ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS   85 ( 2 )   178 - 184   1998年2月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:MOSBY-YEAR BOOK INC  

    A rare case of oral adenosquamous carcinoma in a 78-year-old woman is reported. The tumor occurred in her tongue and metastasized to the submandibular and cervical lymph node;. Histologically, the tumor showed invasive growth involving the submucosal and muscle lavers. Its solid carcinomatous nests exhibited ductal differentiation in the deeper aspects and squamous differentiation toward the surface. Histochemical examination revealed an accumulation of acid mucopolysaccharide in the ductal luminal and the ductal cells were immunohistochemically positive for carcinoembryonic antigen, epithelial membrane antigen, cancer antigen 15-3 and Ulex europaeus agglutinin I. Ultrastructurally, tonotibrils, desmosomes and numerous cytoplasmic processes were common features of the tumor cells. In addition, true glandular structures and pseudocysts were seen in areas. Clinical features of 13 adenosquamous carcinomas in the literature were analyzed.

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  • Immunohistochemical characterization of composite human oral mucosal equivalent

    Journal of Oral and Maxillofacial Surgery   56 ( 4(補冊) )   99   1998年

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  • Development of human composite oral mucosal grafts.

    G Takacs, K Izumi, LM Rhodes, SE Feinberg

    JOURNAL OF DENTAL RESEARCH   77   194 - 194   1998年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER ASSOC DENTAL RESEARCH  

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  • Computed tomographic study on postoperative changes of reconstructed mandibular condyle following costochondral graft Report of case

    Asian Journal of oral and maxillofacial Surgery   8 ( 2 )   171   1996年

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  • 肋骨付き軟骨による下顎頭再建術後のCT上の変化について,症例報告

    アジア口腔外科学会雑誌   8 ( 2 )   171   1996年

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  • PRIMARY LEIOMYOSARCOMA OF THE MAXILLA WITH REGIONAL LYMPH-NODE METASTASIS - REPORT OF A CASE AND REVIEW OF THE LITERATURE

    K IZUMI, T MAEDA, J CHENG, T SAKU

    ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS   80 ( 3 )   310 - 319   1995年9月

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    記述言語:英語   出版者・発行元:MOSBY-YEAR BOOK INC  

    A rare case of oral leiomyosarcoma diagnosed with the aid of immunohistochemical and electron microscopic examinations together with a review of the literature are reported. The patient was a 70-year-old Japanese man. The primary tumor involved the maxillary gingiva and bone and metastasized to the cervical lymph nodes. On histologic examination the tumor showed invasive growth into the maxillary bone. It was composed of interlacing fascicles of spindle-shaped cells with eosinophilic cytoplasm and elongated, blunt-ended nuclei. The tumor formed extensive metastatic foci in the cervical lymph nodes. On immunohistochemical examination most of the tumor cells were positive for desmin, smooth muscle-specific actin, and myosin. The ultrastructural characteristics of the tumor cells were abundant microfilaments, pinocytotic vesicles, and basement membrane formation. The findings were indicative of a tumor demonstrating myogenic differentiation. A review of the literature during the past 50 years disclosed a total of 60 oral leiomyosarcomas, including our case.

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  • Ultrastructural and immunocytochemical characterization of a gingival neurofibroma.

    Asian Journal of oral and maxillofacial surgery   1995年

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  • Responses of neural elements in the experimentally induced carcinoma of the hamster tongue

    Japanese Journal of Oral Maxillofacial Surgery   38 ( 6 )   1992年

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  • 実験的ハムスター舌癌組織における神経線維の動態

    日本口腔外科学会雑誌   38 ( 6 )   1992年

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  • CALCITONIN GENE-RELATED PEPTIDE-IMMUNOREACTIVE NERVES IN CARCINOMA EXPERIMENTALLY INDUCED IN THE HAMSTER TONGUE

    K IZUMI, T MAEDA, T NAKAJIMA

    BIOMEDICAL RESEARCH-TOKYO   12 ( 6 )   377 - 390   1991年12月

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    記述言語:英語   出版者・発行元:BIOMED RES FOUND  

    Innervation to carcinomas induced by 9,10-dimethyl-1,2-benzanthracene (DMBA) in the hamster tongue was investigated by means of immunohistochemistry for calcitonin gene-related peptide (CGRP). The application of DMBA induced papillomatous tumors with epithelial dysplasia and then well-differentiated squamous cell carcinomas showing cord-like invasion of the muscle layer developed under the papillomatous portion of the epithelium. The characteristic features of the innervation were the scarcity of CGRP-positive nerve fibers in the carcinomatous stroma and the loss of their relationships with blood vessels. In contrast, numerous fine CGRP-positive nerves which were associated with blood vessels and carcinoma nests were present in the carcinoma invading zone where infiltration of lymphocytes, neutrophils, mast cells and macrophages was recognized. In addition, a dense distribution of intensely immunopositive nerves was seen around arterioles in the invasion zone and in the surrounding muscle layer. The results indicate possible involvement of the CGRP-positive nerves in the dull pain associated with malignant tumors and cellular responses at the site of tumor invasion, and in providing sufficient blood supply to the tumor tissue which is devoid of autoregulatory mechanism of the blood flow.

    DOI: 10.2220/biomedres.12.377

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講演・口頭発表等

  • Current and Future Research Topics on Tissue Engineering of Oral Mucosa-

    泉 健次

    The International Collaborative Symposium on Development of Human Resources in Practical Oral Health and Treatment  2019年2月 

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    会議種別:口頭発表(基調)  

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  • Oral & Maxillofacial Tissue Enigineering and Reconstruction - Oral Mucosa -

    泉 健次

    The Internartional Conference on Dental Science & Training 2018 “Future Trends in Dentistry”  2018年12月 

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    会議種別:口頭発表(招待・特別)  

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  • Clinical Applications of Oral Keratinocytes: Bench to Bedside, then back to the Bench 招待

    泉 健次

    35th Annual Scientific Meeting@The University of Hong Kong  2017年12月 

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  • Tissue Engineering of Oral Mucosa 招待

    泉 健次

    Gadyah Mada University Faculty Meeting  2017年8月 

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  • うろこコラーゲンを足場とする培養口腔粘膜作成 招待

    泉 健次

    日本バイオマテリアル学会シンポジウム2016  2016年11月 

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  • A novel approach to identify oral keratinocyte stem cells 招待

    泉 健次

    39th annual scientific meeting of association for dental sciences of the republic of china39th annual scientific meeting of association for dental sciences of the republic of china  2016年9月 

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  • 口腔粘膜上皮幹細胞の単離・同定に向けたアプローチ 招待

    泉 健次

    第121回日本解剖学会総会  2016年3月 

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  • 3D In Vitro Models As Alternatives to Animal Testng 招待

    泉 健次

    International Symposium on Development of Human Resources in Practical Oral Health and Treatment.  2016年1月 

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  • 培養口腔粘膜の臨床応用 招待

    泉 健次

    日本セラミック協会第28回秋季シンポジウム  2015年9月 

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産業財産権

  • 口腔粘膜上皮細胞培養用の架橋線維化コラーゲンゲル

    泉 健次, 鈴木絢子, 三輪慶人, 桑江博之, 水野潤, 兒玉泰洋, 山口 勇

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    出願番号:特願2018-242505  出願日:2018年12月

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  • 細胞の品質評価方法、品質評価システム及び品質評価プログラム

    泉 健次, 干川絵美, 佐藤大祐, 木森義隆

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    出願番号:特願2018-149488  出願日:2018年8月

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  • 線維化コラーゲンゲル作製用鋳型材料

    泉 健次, 三輪慶人, 桑江博之, 水野潤, 児玉泰洋, 山口勇

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    出願番号:特願2018-145182  出願日:2018年8月

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  • 細胞培養方法及び培養組織

    泉 健次, 加藤 寛子, 前田 竜, 河上 貴宏, 山口 勇

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    出願人:国立大学法人 新潟大学, 多木化学株式会社

    出願番号:特願2016-031777  出願日:2016年2月

    公開番号:特開2017-147951  公開日:2017年8月

    特許番号/登録番号:特許6758616  登録日:2020年9月 

    権利者:国立大学法人 新潟大学, 多木化学株式会社

    J-GLOBAL

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Works(作品等)

  • Clinical application of oral mucosa equivalent

    1999年

     詳細を見る

  • ヒト培養口腔粘膜の臨床応用

    1999年

     詳細を見る

共同研究・競争的資金等の研究

  • 足場材の硬さの違いを利用した上皮角化・非角化様式解明と培養口腔粘膜作成法への応用

    研究課題/領域番号:20H03870  2020年4月 - 2023年3月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    泉 健次

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    担当区分:研究代表者 

    配分額:17680000円 ( 直接経費:13600000円 、 間接経費:4080000円 )

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  • 培養口腔粘膜作成用マイクロパターン化魚コラーゲン足場材の開発

    2020年4月 - 2021年3月

    北海道大学病院 臨床研究開発センター  橋渡し研究戦略的推進プログラム  革新的医療技術創出に関するシーズA

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    担当区分:研究代表者 

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  • 細胞運動能を指標とした再生医療向け非侵襲的口腔粘膜上皮細胞評価システムの開発

    2017年 - 2019年

    日本学術振興会  基盤研究(B) 

    泉 健次, 医歯学系

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    担当区分:研究代表者  資金種別:競争的資金

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  • 低酸素発光プローブを利用した培養口腔粘膜作成過程に最適な低酸素ニッチ環境の確立

    2014年 - 2017年

    日本学術振興会  基盤研究(B) 

    泉 健次, 医歯学系

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

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  • 口腔粘膜上皮の免疫特権性を利用した口腔扁平苔癬インビトロモデルと治療法の開発

    2014年 - 2016年

    日本学術振興会  挑戦的萌芽研究 

    泉 健次, 医歯学系

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

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  • エイジレスな培養口腔粘膜上皮の開発・作成

    2011年 - 2013年

    日本学術振興会  挑戦的萌芽研究 

    泉 健次, 医歯学系

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

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  • オートファジーの薬学的操作による口腔粘膜前駆/幹細胞の抗老化・維持システムの確立

    2010年 - 2012年

    日本学術振興会  基盤研究(B) 

    泉 健次, 医歯学系

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

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  • Development of human oral mucosa equivalent

      詳細を見る

    資金種別:競争的資金

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  • The relationship between apoptosis and oral mucosal lesion

      詳細を見る

    資金種別:競争的資金

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  • Oral mucosal keratinocyte culture and application of oral mucosal reconstruction

      詳細を見る

    資金種別:競争的資金

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  • The histological Study on the replacement of a collagen material in rabbit TMJ.

      詳細を見る

    資金種別:競争的資金

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▶ 全件表示

 

担当経験のある授業科目

  • 「食べる」

    2021年
    -
    現在
    機関名:新潟大学

  • 歯科理工学Ⅱ

    2020年
    -
    現在
    機関名:新潟大学

  • 基礎歯学コースワーク(生体材料関係コースワークⅡ)

    2020年
    -
    現在
    機関名:新潟大学

  • 生体材料学

    2018年
    -
    現在
    機関名:新潟大学

  • 生体理工学

    2018年
    -
    現在
    機関名:新潟大学

  • 生体組織再生工学特論ⅠA

    2018年
    機関名:新潟大学

  • 生体組織再生工学演習ⅡA

    2018年
    機関名:新潟大学

  • 生体組織再生工学特論ⅡA

    2018年
    機関名:新潟大学

  • 基礎歯学コースワーク(生体組織再生工学コースワークⅠ)

    2017年
    -
    現在
    機関名:新潟大学

  • 基礎歯学コースワーク(生体組織再生工学コースワークⅡ)

    2017年
    -
    2018年
    機関名:新潟大学

  • 生体組織再生工学演習ⅠA

    2017年
    -
    2018年
    機関名:新潟大学

  • 生体組織再生工学演習ⅠB

    2017年
    機関名:新潟大学

  • 生体組織再生工学特論ⅡB

    2017年
    機関名:新潟大学

  • 生体組織再生工学演習ⅡB

    2017年
    機関名:新潟大学

  • 「食べる」

    2016年
    -
    現在
    機関名:新潟大学

  • 歯学研究入門

    2016年
    -
    2017年
    機関名:新潟大学

  • 早期臨床実習Ⅱ

    2015年
    -
    現在
    機関名:新潟大学

  • 歯科理工学Ⅰ

    2014年
    -
    現在
    機関名:新潟大学

  • 人体のしくみ

    2010年
    -
    2014年
    機関名:新潟大学

  • 口腔組織発生学

    2010年
    -
    2013年
    機関名:新潟大学

  • 人体発生学

    2010年
    -
    2011年
    機関名:新潟大学

  • 基礎科学演習

    2009年
    -
    2016年
    機関名:新潟大学

  • 組織学総論

    2009年
    -
    2013年
    機関名:新潟大学

  • 組織学各論

    2009年
    -
    2013年
    機関名:新潟大学

  • 神経解剖学

    2009年
    機関名:新潟大学

  • 形態解析学演習

    2009年
    機関名:新潟大学

  • 基礎歯学コースワーク(ベーシック培養細胞実験コース)

    2009年
    機関名:新潟大学

  • 骨筋学

    2009年
    機関名:新潟大学

  • 脈管内臓学

    2009年
    機関名:新潟大学

  • 感覚器学

    2009年
    機関名:新潟大学

▶ 全件表示