Graduate School of Science and Technology Life and Food Sciences Professor
Faculty of Science Department of Science Professor
Updated on 2024/12/13
博士(医学) ( 1992.3 大阪大学 )
理学修士 ( 1988.3 大阪大学 )
理学士 ( 1986.3 大阪大学 )
biochemistry
glycobiology
carbohydrate chemistry
developmental biology
zebrafish
glycomics
evolution
glycan
glycosyltransferase
phylogeny
database
Glycan Sequencer
Life Science / Functional biochemistry
Life Science / Structural biochemistry
Niigata University Department of Biology, Faculty of Science Professor
2009.9
Osaka University Department of Chemistry, Graduate School of Science Associate Professor
2004.3 - 2009.8
Kyoto Institute of Technology Department of Applied Biology Associate Professor
2003.10 - 2004.2
Kyoto Institute of Technology Department of Applied Biology Assistant Professor
1999.3 - 2003.9
University of Michigan Howard Hughes Medical Institute Research Associate
1992.10 - 1994.9
Osaka University Department of Chemistry, Faculty of Science Research Associate
1992.4 - 1999.2
Niigata University Faculty of Science Department of Science Professor
2017.4
Niigata University Graduate School of Science and Technology Life and Food Sciences Professor
2009.9
Niigata University Graduate School of Science and Technology Life and Food Sciences Professor
2009.9
Niigata University Abolition organization Molecular and Cellular Science Professor
2009.9 - 2017.3
Osaka University Graduate School of Medicine Department of Physiology
1988.4 - 1992.3
Country: Japan
Osaka University Graduate School of Science Department of Organic Chemistry
1986.4 - 1988.3
Country: Japan
Osaka University Faculty of Science Department of Chemistry
1982.4 - 1986.3
Country: Japan
THE JAPANESE BIOCHEMICAL SOCIETY
THE JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH
THE JAPANESE SOCIETY OF APPLIED GLYCOSCIENCE
Increased levels of acidic free-N-glycans, including multi-antennary and fucosylated structures, in the urine of cancer patients. International journal
Ken Hanzawa, Miki Tanaka-Okamoto, Hiroko Murakami, Noriko Suzuki, Mikio Mukai, Hidenori Takahashi, Takeshi Omori, Kenji Ikezawa, Kazuyoshi Ohkawa, Masayuki Ohue, Shunji Natsuka, Yasuhide Miyamoto
PloS one 17 ( 4 ) e0266927 2022
Toward robust N-glycomics of various tissue samples that may contain glycans with unknown or unexpected structures Reviewed
Noriko Suzuki, Tatsuya Abe, Ken Hanzawa, Shunji Natsuka
Scientific Reports 11 6334 2021.3
Sequential modification of glycans by linkage-specific alkylamidation of sialic acids and permethylation Reviewed International journal
Tatsuya Abe, Akihiko Kameyama, Shunji Natsuka, Noriko Suzuki
Analytical Biochemistry 606 113861 - 113861 2020.10
Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea. Reviewed International journal
Yoriko Nonomura, Seishiro Sawamura, Ken Hanzawa, Takashi Nishikaze, Sadanori Sekiya, Taiga Higuchi, Fumiaki Nin, Satoru Uetsuka, Hidenori Inohara, Shujiro Okuda, Eiji Miyoshi, Arata Horii, Sugata Takahashi, Shunji Natsuka, Hiroshi Hibino
Scientific reports 9 ( 1 ) 1551 - 1551 2019.2
Quantitative LC-MS and MS/MS analysis of sialylated glycans modified by linkage-specific alkylamidation Reviewed
Noriko Suzuki, Tatsuya Abe, Shunji Natsuka
Analytical Biochemistry 567 117 - 127 2019
Preparation of a molecular library of branched β-glucan oligosaccharides derived from laminarin Reviewed
Shunji Natsuka, Aki Tachibana, Wataru Sumiyoshi, Shin-ichi Nakakita, Noriko Suzuki
Journal of Applied Glycoscience 65 ( 4 ) 45 - 49 2018
Structures and developmental alterations of N-glycans of zebrafish embryos Reviewed
Ken Hanzawa, Noriko Suzuki, Shunji Natsuka
GLYCOBIOLOGY 27 ( 3 ) 228 - 245 2017.3
Improved Method for Drawing of a Glycan Map, and the First Page of Glycan Atlas, Which Is a Compilation of Glycan Maps for a Whole Organism Reviewed
Shunji Natsuka, Mayumi Masuda, Wataru Sumiyoshi, Shin-ichi Nakakita
PLOS ONE 9 ( 7 ) e102219 2014.7
Determination of galactosamine impurities in heparin sodium using fluorescent labeling and conventional high-performance liquid chromatography Reviewed
Satsuki Itoh, Yoko Hiruta, Noritaka Hashii, Naho Fujita, Toru Natsuga, Toshiaki Hattori, Aya Bando, Yuko Sekimoto, Kazuyoshi Miyata, Hiroshi Namekawa, Kazunori Mabuchi, Toru Sakai, Hirotoshi Shimahashi, Kenzo Kawai, Hikaru Yoden, Sadatoshi Koyama, Susanne Odgaard Herr, Shunji Natsuka, Teruhide Yamaguchi, Nana Kawasaki
BIOLOGICALS 41 ( 6 ) 355 - 363 2013.11
Hypothetical Roles of Glycan Diversity in Evolution Reviewed
Shunji Natsuka
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY 25 ( 143 ) 125 - 131 2013.5
One-Step Purification Method for Pyridylamino Glycans Reviewed
Shunji Natsuka
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 76 ( 10 ) 1982 - 1983 2012.10
A Convenient Purification Method for Pyridylamino Monosaccharides Reviewed
Shunji Natsuka
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 75 ( 7 ) 1405 - 1407 2011.7
A comparative study of monosaccharide composition analysis as a carbohydrate test for biopharmaceuticals Reviewed
Akira Harazono, Tetsu Kobayashi, Nana Kawasaki, Satsuki Itoh, Minoru Tada, Noritaka Hashii, Akiko Ishii, Teruyo Arato, Shigehiro Yanagihara, Yuki Yagi, Akiko Koga, Yuriko Tsuda, Mikiko Kimura, Masashi Sakita, Satoshi Kitamura, Hideto Yamaguchi, Hisashi Mimura, Yoshimi Murata, Yasuki Hamazume, Takayuki Sato, Shunji Natsuka, Kazuaki Kakehi, Mitsuhiro Kinoshita, Sakie Watanabe, Teruhide Yamaguchi
BIOLOGICALS 39 ( 3 ) 171 - 180 2011.5
beta-Galactosidases from Jack Bean and Streptococcus Have Different Cleaving Abilities towards Fucose-Containing Sugars Reviewed
Tomoharu Takeuchi, Ken-ichi Sugiura, Kazusa Nishiyama, Hideyo Takahashi, Hideaki Natsugari, Yoichiro Akata, Shunji Natsuka, Ken-ichi Kasai
BIOLOGICAL & PHARMACEUTICAL BULLETIN 34 ( 4 ) 567 - 569 2011.4
Structural analysis of N-glycans of the planarian Dugesia japonica Reviewed
Shunji Natsuka, Yukiko Hirohata, Shin-ichi Nakakita, Wataru Sumiyoshi, Sumihiro Hase
FEBS JOURNAL 278 ( 3 ) 452 - 460 2011.2
N-Glycosylation patterns of hemolymph glycoproteins from Biomphalaria glabrata strains expressing different susceptibility to Schistosoma mansoni infection Reviewed
Tobias Lehr, Sandra Frank, Shunji Natsuka, Hildegard Geyer, Knut Beuerlein, Michael J. Doenhoff, Sumihiro Hase, Rudolf Geyer
EXPERIMENTAL PARASITOLOGY 126 ( 4 ) 592 - 602 2010.12
Involvement of murine beta-1,4-galactosyltransferase V in lactosylceramide biosynthesis Reviewed
Tadahiro Kumagai, Takeshi Sato, Shunji Natsuka, Yukito Kobayashi, Dapeng Zhou, Tadashi Shinkai, Satoru Hayakawa, Kiyoshi Furukawa
GLYCOCONJUGATE JOURNAL 27 ( 7-9 ) 685 - 695 2010.10
The och1 Mutant of Schizosaccharomyces pombe Produces Galactosylated Core Structures of N-Linked Oligosaccharides Reviewed
Takao Ohashi, Yuka Ikeda, Naotaka Tanaka, Shin-ichi Nakakita, Shunji Natsuka, Yuko Giga-Hama, Kaoru Takegawa
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 73 ( 2 ) 407 - 414 2009.2
Biochemical characterization of plasma-derived tissue factor pathway inhibitor: post-translational modification of free, full-length form with particular reference to the sugar chain Reviewed
Y. Mori, T. Hamuro, T. Nakashima, T. Hamamoto, S. Natsuka, S. Hase, S. Iwanaga
JOURNAL OF THROMBOSIS AND HAEMOSTASIS 7 ( 1 ) 111 - 120 2009.1
Free oligosaccharides with Lewis x structure expressed in the segmentation period of zebrafish embryo Reviewed
Kazunobu Moriguchi, Tatsuya Takemoto, Takafumi Aoki, Shin-ichi Nakakita, Shunji Natsuka, Sumihiro Hase
JOURNAL OF BIOCHEMISTRY 142 ( 2 ) 213 - 227 2007.8
Structure determination of a sulfated N-glycans, candidate for a precursor of the selectin ligand in bovine lung Reviewed
Tomonori Murakami, Shunji Natsuka, Shin-ichi Nakakita, Sumihiro Hase
GLYCOCONJUGATE JOURNAL 24 ( 4-5 ) 195 - 206 2007.7
Gas-phase pyridylamination of saccharides: Development and applications Reviewed
Shin-Ichi Nakakita, Wataru Sumiyoshi, Nobumitsu Miyanishi, Shunji Natsuka, Sumihiro Hase, Jun Hirabayashi
ANALYTICAL CHEMISTRY 79 ( 7 ) 2674 - 2679 2007.4
Comparative biochemical study of N-linked glycans from skin of a squid, Todarodes pacificus Reviewed
Shunji Natsuka, Miwa Ishida, Akira Ichikawa, Koji Ikura, Sumihiro Hase
JOURNAL OF BIOCHEMISTRY 140 ( 1 ) 87 - 93 2006.7
EDEM3, a soluble EDEM homolog, enhances glycoprotein endoplasmic reticulum-associated degradation and mannose trimming Reviewed
K Hirao, Y Natsuka, T Tamura, Wada, I, D Morito, S Natsuka, P Romero, B Sleno, LO Tremblay, A Herscovics, K Nagata, N Hosokawa
JOURNAL OF BIOLOGICAL CHEMISTRY 281 ( 14 ) 9650 - 9658 2006.4
Structural diversity of cytosolic free oligosaccharides in the human hepatoma cell line, HepG2 Reviewed
K Yanagida, S Natsuka, S Hase
GLYCOBIOLOGY 16 ( 4 ) 294 - 304 2006.4
Alteration of brain type N-glycans in neurological mutant mouse brain Reviewed
S Nakakita, S Natsuka, J Okamoto, K Ikenaka, S Hase
JOURNAL OF BIOCHEMISTRY 138 ( 3 ) 277 - 283 2005.9
Comparative biochemical view of N-glycans Reviewed
S Natsuka
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY 17 ( 97 ) 229 - 236 2005.9
Characterization of wheat germ agglutinin ligand on soluble glycoproteins in Caenorhabditis elegans Reviewed
S Natsuka, M Kawaguchi, Y Wada, A Ichikawa, K Ikura, S Hase
JOURNAL OF BIOCHEMISTRY 138 ( 2 ) 209 - 213 2005.8
Expression of complex-type N-glycans in developmental periods of zebrafish embryo Reviewed
T Takemoto, S Natsuka, S Nakakita, S Hase
GLYCOCONJUGATE JOURNAL 22 ( 1-2 ) 21 - 26 2005.2
In vitro modification of betaine-homocysteine S-methyltransferase by tissue-type transglutaminase Reviewed
A Ichikawa, Y Ohashi, S Terada, S Natsuka, K Ikura
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 36 ( 10 ) 1981 - 1992 2004.10
Precise recognition of “brain-type” 1-4galactosyltransferase Reviewed
Nakakita S, Natsuka S, Ikenaka K, Hase S
Japan Journal of Electrophoresis 48 ( 1 ) 1 - 4 2004
Caenorhabditis elegans as a model animal of glycobiology: accumulation of structure information Reviewed
S Natsuka
SEIKAGAKU 75 ( 2 ) 131 - 133 2003.2
Identification of an endo-beta-N-acetylglucosaminidase gene in Caenorhabditis elegans and its expression in Escherichia coli Reviewed
T Kato, K Fujita, M Takeuchi, K Kobayashi, S Natsuka, K Ikura, H Kumagai, Y Yamamoto
GLYCOBIOLOGY 12 ( 10 ) 581 - 587 2002.10
Co(II)-regulated substrate specificity of cytosolic alpha-mannosidase Reviewed
M Yamagishi, T Ishimizu, S Natsuka, S Hase
JOURNAL OF BIOCHEMISTRY 132 ( 2 ) 253 - 256 2002.8
Inhibition of transglutaminase by synthetic tyrosine melanin Reviewed
K Ikura, C Otomo, S Natsuka, A Ichikawa, K Wakamatsu, S Ito, S Taoguchi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 66 ( 6 ) 1412 - 1414 2002.6
Structural analysis of N-linked Glycans in Caenorhabditis elegans Reviewed
S Natsuka, J Adachi, M Kawaguchi, S Nakakita, S Hase, A Ichikawa, K Ikura
JOURNAL OF BIOCHEMISTRY 131 ( 6 ) 807 - 813 2002.6
Method for purification of fluorescence-labeled oligosaccharides by pyridylamination Reviewed
S Natsuka, J Adachi, M Kawaguchi, A Ichikawa, K Ikura
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 66 ( 5 ) 1174 - 1175 2002.5
Analysis of oligosaccharide structures of glycoproteins in polyacrylamide gel Reviewed
S Nakakita, D Ama, S Natsuka, S Hase
ANALYTICAL BIOCHEMISTRY 303 ( 2 ) 206 - 209 2002.4
A monoclonal antibody to cyclomaltoheptaose (beta-cyclodextrin): Characterization and use for immunoassay of beta-cyclodextrin and its derivatives Reviewed
K Ikura, J Fujimoto, K Kubonishi, S Natsuka, H Hashimoto, T Ito, K Fujita
CYTOTECHNOLOGY 40 ( 1-3 ) 23 - 29 2002
Co-overexpression of folding modulators improves the solubility of the recombinant guinea pig liver transglutaminase expressed in Escherichia coli Reviewed
K Ikura, T Kokubu, S Natsuka, A Ichikawa, M Adachi, K Nishihara, H Yanagi, S Utsumi
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 32 ( 2 ) 189 - 205 2002
Analysis of glycan expression by fluorescence labeling method Reviewed
Natsuka S
Japan Journal of Electrophoresis 46 ( 2 ) 35 - 38 2002
Molecular cloning and expression of Caenorhabditis elegans ERp57-homologue with transglutaminase activity Reviewed
S Natsuka, R Takubo, R Seki, K Ikura
JOURNAL OF BIOCHEMISTRY 130 ( 6 ) 731 - 735 2001.12
Structural analysis of the sugar chains of human urinary thrombomodulin Reviewed
H Wakabayashi, S Natsuka, M Honda, M Naotsuka, Y Ito, J Kajihara, S Hase
JOURNAL OF BIOCHEMISTRY 130 ( 4 ) 543 - 552 2001.10
Chemical structures of C. elegans O-glycans Reviewed
S Natsuka
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY 13 ( 73 ) 551 - 553 2001.9
High molecular weight transglutaminase inhibitor produced by a microorganism (Streptomyces lavendulae Y-200) Reviewed
K Ikura, K Minami, C Otomo, H Hashimoto, S Natsuka, K Oda, K Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 64 ( 1 ) 116 - 124 2000.1
beta 1-4Galactosyltransferase activity of mouse brain as revealed by analysis of brain-specific complex-type N-linked sugar chains Reviewed
S Nakakita, KK Menon, S Natsuka, K Ikenaka, S Hase
JOURNAL OF BIOCHEMISTRY 126 ( 6 ) 1161 - 1169 1999.12
A pyridylamination method aimed at automatic oligosaccharide analysis of N-linked sugar chains Reviewed
K Yanagida, S Natsuka, S Hase
ANALYTICAL BIOCHEMISTRY 274 ( 2 ) 229 - 234 1999.10
Presence of asparagine-linked N-acetylglucosamine and chitobiose in Pyrus pyrifolia S-RNases associated with gametophytic self-incompatibility Reviewed
T Ishimizu, Y Mitsukami, T Shinkawa, S Natsuka, S Hase, M Miyagi, F Sakiyama, S Norioka
EUROPEAN JOURNAL OF BIOCHEMISTRY 263 ( 3 ) 624 - 634 1999.8
Molecular cloning and characterization of two zebrafish alpha(1,3)fucosyltransferase genes developmentally regulated in embryogenesis Reviewed
N Kageyama, S Natsuka, S Hase
JOURNAL OF BIOCHEMISTRY 125 ( 4 ) 838 - 845 1999.4
Novel proteoglycan linkage tetrasaccharides of human urinary soluble thrombomodulin, SO4-3GlcA beta 1-3Gal beta 1-3(+/- Sia alpha 2-6)Gal beta 1-4Xyl Reviewed
H Wakabayashi, S Natsuka, T Mega, N Otsuki, M Isaji, M Naotsuka, S Koyama, T Kanamori, K Sakai, S Hase
JOURNAL OF BIOLOGICAL CHEMISTRY 274 ( 9 ) 5436 - 5442 1999.2
Partial purification and characterization of a novel endo-beta-mannosidase acting on N-linked sugar chains from Lilium longflorum thumb Reviewed
A Sasaki, M Yamagishi, T Mega, S Norioka, S Natsuka, S Hase
JOURNAL OF BIOCHEMISTRY 125 ( 2 ) 363 - 367 1999.2
Systematic analysis of N-linked sugar chains from whole tissue employing partial automation Reviewed
Fujimoto, I, KK Menon, Y Otake, F Tanaka, H Wada, H Takahashi, S Tsuji, S Natsuka, S Nakakita, S Hase, K Ikenaka
ANALYTICAL BIOCHEMISTRY 267 ( 2 ) 336 - 343 1999.2
Development-dependent expression of complex-type sugar chains specific to mouse brain Reviewed
S Nakakita, S Natsuka, K Ikenaka, S Hase
JOURNAL OF BIOCHEMISTRY 123 ( 6 ) 1164 - 1168 1998.6
Analysis of N- and O-glycans by pyridylamination.
S. Natsuka, S. Hase
Methods in molecular biology (Clifton, N.J.) 76 101 - 113 1998
Purification and characterization of neutral alpha-mannosidase from hen oviduct: Studies on the activation mechanism of Co2+ Reviewed
K Yamashiro, H Itoh, M Yamagishi, S Natsuka, T Mega, S Hase
JOURNAL OF BIOCHEMISTRY 122 ( 6 ) 1174 - 1181 1997.12
Structures of the sugar chains of recombinant macrophage colony-stimulating factor produced in Chinese hamster ovary cells Reviewed
Y Ushida, S Natsuka, Y Shimokawa, Z Takatsu, S Shimamura, S Hase
JOURNAL OF BIOCHEMISTRY 122 ( 1 ) 148 - 156 1997.7
Detection of UDP-D-Xylose: alpha-D-xyloside alpha 1->3Xylosyltransferase activity in human hepatoma cell line HepG2 Reviewed
S Minamida, K Aoki, S Natsuka, K Omichi, K Fukase, S Kusumoto, S Hase
JOURNAL OF BIOCHEMISTRY 120 ( 5 ) 1002 - 1006 1996.11
The fucosyltransferase FucT-VII regulates E-selectin ligand synthesis in human T cells Reviewed
RN Knibbs, RA Craig, S Natsuka, A Chang, M Cameron, JB Lowe, LM Stoolman
JOURNAL OF CELL BIOLOGY 133 ( 4 ) 911 - 920 1996.5
Expression of the alpha(1,3)fucosyltransferase Fuc-TVII in lymphoid aggregate high endothelial venules correlates with expression of L-selectin ligands Reviewed
PL Smith, KM Gersten, B Petryniak, RJ Kelly, C Rogers, Y Natsuka, JA Alford, EP Scheidegger, S Natsuka, JB Lowe
JOURNAL OF BIOLOGICAL CHEMISTRY 271 ( 14 ) 8250 - 8259 1996.4
MOLECULAR-CLONING, EXPRESSION, CHROMOSOMAL ASSIGNMENT, AND TISSUE-SPECIFIC EXPRESSION OF A MURINE ALPHA-(1,3)-FUCOSYL-TRANSFERASE LOCUS CORRESPONDING TO THE HUMAN ELAM-1 LIGAND FUCOSYL-TRANSFERASE Reviewed
KM GERSTEN, S NATSUKA, M TRINCHERA, B PETRYNIAK, RJ KELLY, N HIRAIWA, NA JENKINS, DJ GILBERT, NG COPELAND, JB LOWE
JOURNAL OF BIOLOGICAL CHEMISTRY 270 ( 42 ) 25047 - 25056 1995.10
FUCOSYL-TRANSFERASES SERVING IN BIOSYNTHESIS OF SELECTIN LIGANDS Reviewed
S NATSUKA
SEIKAGAKU 67 ( 5 ) 368 - 372 1995.5
ENZYMES INVOLVED IN MAMMALIAN OLIGOSACCHARIDE BIOSYNTHESIS Reviewed
S NATSUKA, JB LOWE
CURRENT OPINION IN STRUCTURAL BIOLOGY 4 ( 5 ) 683 - 691 1994.10
MOLECULAR-CLONING OF A CDNA-ENCODING A NOVEL HUMAN-LEUKOCYTE ALPHA-1,3-FUCOSYL-TRANSFERASE CAPABLE OF SYNTHESIZING THE SIALYL-LEWIS-X DETERMINANT Reviewed
S NATSUKA, KM GERSTEN, K ZENITA, R KANNAGI, JB LOWE
JOURNAL OF BIOLOGICAL CHEMISTRY 269 ( 24 ) 16789 - 16794 1994.6
STRUCTURE-FUNCTION ANALYSIS OF HUMAN IL-6 RECEPTOR - DISSOCIATION OF AMINO-ACID-RESIDUES REQUIRED FOR IL-6-BINDING AND FOR IL-6 SIGNAL TRANSDUCTION THROUGH GP130 Reviewed
H YAWATA, K YASUKAWA, S NATSUKA, M MURAKAMI, K YAMASAKI, M HIBI, T TAGA, T KISHIMOTO
EMBO JOURNAL 12 ( 4 ) 1705 - 1712 1993.4
MACROPHAGE DIFFERENTIATION-SPECIFIC EXPRESSION OF NF-IL6, A TRANSCRIPTION FACTOR FOR INTERLEUKIN-6 Reviewed
S NATSUKA, S AKIRA, Y NISHIO, S HASHIMOTO, T SUGITA, H ISSHIKI, T KISHIMOTO
BLOOD 79 ( 2 ) 460 - 466 1992.1
REGULATION OF EXPRESSION OF THE INTERLEUKIN-6 GENE - STRUCTURE AND FUNCTION OF THE TRANSCRIPTION FACTOR NF-IL6 Reviewed
S AKIRA, H ISSHIKI, T NAKAJIMA, S KINOSHITA, Y NISHIO, S NATSUKA, T KISHIMOTO
CIBA FOUNDATION SYMPOSIA 167 47 - 67 1992
A NUCLEAR FACTOR FOR THE IL-6 GENE (NF-IL6) Reviewed
S AKIRA, H ISSHIKI, T NAKAJIMA, S KINOSHITA, Y NISHIO, S HASHIMOTO, S NATSUKA, T KISHIMOTO
CHEMICAL IMMUNOLOGY 51 299 - 322 1992
AUGMENTATION OF HAPTOGLOBIN PRODUCTION IN HEP3B CELL-LINE BY A NUCLEAR FACTOR NF-IL6 Reviewed
S NATSUKA, H ISSHIKI, S AKIRA, T KISHIMOTO
FEBS LETTERS 291 ( 1 ) 58 - 62 1991.10
INTERLEUKIN-6 RECEPTOR AND A UNIQUE MECHANISM OF ITS SIGNAL TRANSDUCTION Reviewed
T TAGA, M HIBI, Y HIRATA, H YAWATA, S NATSUKA, K YASUKAWA, T TOTSUKA, K YAMASAKI, T HIRANO, T KISHIMOTO
IMMUNOLOGICAL RECOGNITION, PTS 1 AND 2 54 713 - 722 1989
STRUCTURES OF SUGAR CHAINS OF A PARA-NITROPHENYL ACETATE-HYDROLYZING ESTERASE FROM THE MICROSOMES OF RAT-LIVER Reviewed
S NATSUKA, M HIMENO, S HASE, H ITO, T UEDA, K KATO, T IKENAKA
JOURNAL OF BIOCHEMISTRY 103 ( 6 ) 986 - 991 1988.6
IDENTIFICATION METHOD FOR 12 OLIGOMANNOSE-TYPE SUGAR CHAINS THOUGHT TO BE PROCESSING INTERMEDIATES OF GLYCOPROTEINS Reviewed
S HASE, S NATSUKA, H OKU, T IKENAKA
ANALYTICAL BIOCHEMISTRY 167 ( 2 ) 321 - 326 1987.12
FLUORESCENCE METHOD FOR THE STRUCTURAL-ANALYSIS OF OLIGOMANNOSE-TYPE SUGAR CHAINS BY PARTIAL ACETOLYSIS Reviewed
S NATSUKA, S HASE, T IKENAKA
ANALYTICAL BIOCHEMISTRY 167 ( 1 ) 154 - 159 1987.11
Glycoscience : basic science to applications : insights from the Japan consortium for glycobiology and glycotechnology (JCGG)
Shunji Natsuka( Role: Contributor , Genetic modification: small fish (zebrafish and medaka))
Springer 2019 ( ISBN:9789811358555 )
未来を創るグライコサイエンス ―我が国のロードマップ―
長束俊治( Role: Contributor , 糖鎖機能解析のキーテクノロジー:遺伝子改変:小型魚類(4))
日本糖鎖科学コンソーシアム 2018.5
生物薬科学実験講座,糖質Ⅱ糖タンパク質実験法
長束 俊治, 長谷 純宏( Role: Contributor , 二次元HPLCマッピングと溶出の規則性,プロトン核磁気共鳴(1H-NMR))
廣川書店 2011
ピリジルアミノ化による糖鎖解析-糖鎖多様性の解析に向けて
長谷 純宏, 長束 俊治, 中北 愼一, 石水 毅( Role: Joint author)
大阪大学出版会 2009.3 ( ISBN:4872592697 )
生物薬科学実験講座,糖質Ⅰ基本実験操作法
長束 俊治, 長谷 純宏( Role: Contributor , 蛍光標識による糖組成分析,蛍光標識,PA-糖鎖の逆相カラムによる分離と精製,陰イオン交換HPLCによるPA-糖鎖の分離)
廣川書店 2009
Experimental Glycoscience: Glycochemistry
NATSUKA Shunji( Role: Joint author , “Release of O-glycans by chemical methods”, “Separation of oligosaccharides by 2D HPLC”, “Equilibrium dialysis”)
Springer 2008
タンパク質修飾解析プロトコール
長束 俊治, 長谷 純宏( Role: Joint author , O-結合型糖鎖の構造解析)
羊土社 2005
未来を拓く糖鎖科学
長束 俊治( Role: Joint author , 糖タンパク質よりO-結合型糖鎖の化学的切り出し,オリゴ糖の2次元HPLCによる分離,平衡透析を用いた糖鎖-タンパク質相互作用の解析)
金芳堂 2005
糖鎖科学の新展開
長束 俊治( Role: Joint author , 糖鎖分析の統合オペレーティングシステムとしてのピリジルアミノ化法)
NTS 2005
ポストゲノムの糖鎖生物学がわかる
長束 俊治( Role: Joint author , アスパラギン結合型糖鎖の多様性形成機構)
羊土社 2002
Glycoanalysis Protocols
NATSUKA Shunji, HASE Sumihiro( Role: Joint author , Analysis of N- and O-glycans by pyridylamination)
Humana Press 1998
グライコバイオロジー実験プロトコール
長束 俊治, 長谷 純宏( Role: Joint author , ピリジルアミノ(PA)化法による糖鎖の蛍光標識と分離)
秀潤社 1996
糖―その多様性を探る
長束 俊治, 長谷 純宏( Role: Joint author , ピリジルアミノ化法による糖鎖の高感度分析)
化学同人 1992
希少糖を含む分泌型糖ペプチド Invited Reviewed
長束 俊治
化学と生物 57 ( 1 ) 5 - 6 2019.1
N-Glycosylation Patterns of Hemolymph Glycoproteins from Biomphalaria Glabrata Strains Expressing Different Susceptibility to Schistosoma Mansoni Infection
Tobias Lehr, Sandra Frank, Shunji Natsuka, Hildegard Geyer, Knut Beuerlein, Michael J. Doenhoff, Sumihiro Hase, Rudolf Geyer
GLYCOBIOLOGY 20 ( 11 ) 1524 - 1524 2010.11
Preface for a Special Issue Entitled "Chemical Biology of Glycans"
NATSUKA Shunji
17 ( 97 ) 191 - 192 2005.9
Functional analysis of EDEM homologue protein
Yuko Natsuka, Kazuyoshi Hirao, Junji Nakamura, Taku Tamura, Ikuo Wada, Shunji Natsuka, Nobuko Hosokawa, Kazuhiro Nagata
CELL STRUCTURE AND FUNCTION 30 47 - 47 2005.6
EDEM 可溶性ホモログ EDEM3はマンノシダーゼ活性を介してERADを促進する Reviewed
長束優子, 平尾和義, 中村純治, 田村拓, 和田郁夫, 長束俊治, 細川暢子, 永田和宏
第25回日本糖質学会年会, 大津市, 2005. 7. 20-22 2005
Cloning, expression, and characterization of EDEM homolog proteins
Y Natsuka, K Hirao, J Nakamura, S Natsuka, N Hosokawa, K Nagata
GLYCOBIOLOGY 14 ( 11 ) 1110 - 1111 2004.11
Structure analysis of invertebrate N-glycans by comparative biochemical view
S Natsuka, S Hase
GLYCOBIOLOGY 14 ( 11 ) 1205 - 1205 2004.11
EDEM ホモログ蛋白質 の機能解析 Reviewed
長束優子, 平尾和義, 中村純治, 田村拓, 和田郁夫, 長束俊治, 細川暢子, 永田和宏
第77 回日本生化学会大会(2004.10.15. 横浜市) 2004
Automated analysis of sugar chains of glycoproteins in mouse brains
Fujimoto, I, F Tanaka, S Natsuka, S Hase, K Ikenaka
JOURNAL OF NEUROCHEMISTRY 69 S165 - S165 1997
微量糖蛋白質からのピリジルアミノ化糖鎖の簡便な調製法
柳田 寛太, 長束 俊治, 長谷 純宏
日本分子生物学会年会プログラム・講演要旨集 19 218 - 218 1996.8
FUCOSYL-TRANSFERASES IN THE SYNTHESIS OF SELECTIN LIGANDS ON T-CELL
RN KNIBBS, RA CRAIG, S NATSUKA, A THALL, M CAMERON, JB LOWE, LM STOOLMAN
FASEB JOURNAL 9 ( 3 ) A34 - A34 1995.3
白血球ホーミングにおけるフコース転移酵素の役割
長束 俊治
細胞工学 14 ( 4 ) 437 - 442 1995
MOLECULAR-CLONING OF A CDNA-ENCODING A NOVEL HUMAN-LEUKOCYTE ALPHA-1,3-FUCOSYL-TRANSFERASE CAPABLE OF SYNTHESIZING THE SIALYL-LEWIS-X DETERMINANT (VOL 269, PG 16789, 1994)
S NATSUKA, KM GERSTEN, K ZENITA, R KANNAGI, JB LOWE
JOURNAL OF BIOLOGICAL CHEMISTRY 269 ( 32 ) 20806 - 20806 1994.8
MOLECULAR-GENETICS OF MAMMALIAN FUCOSYL-TRANSFERASES
JB LOWE, DJ LEGAULT, RJ KELLY, PL SMITH, S NATSUKA, Y NATSUKA, P MALY, B PETRYNIAK, D GIORGI, S ROUQUIER, G LENNON, N HIRAIWA
JOURNAL OF CELLULAR BIOCHEMISTRY 259 - 259 1994
STRUCTURE AND FUNCTION OF MAMMALIAN GLYCOSYLTRANSFERASE GENES
JB LOWE, BW WESTON, RJ KELLY, RD LARSEN, Y NATSUKA, S NATSUKA, N HIRAIWA, KM GERSTEN, PL SMITH, DJ LEGAULT
GLYCOCONJUGATE JOURNAL 10 ( 4 ) 232 - 232 1993.8
ピリジルアミノ化法による糖鎖の高感度分析 (糖--その多様性を探る) -- (構造解析・分析)
長束 俊治, 長谷 純宏
化学 増刊 ( 122 ) p125 - 132 1992.12
REGULATION OF EXPRESSION OF THE INTERLEUKIN-6 GENE - STRUCTURE AND FUNCTION OF THE TRANSCRIPTION FACTOR NF-IL6 Reviewed
S AKIRA, H ISSHIKI, T NAKAJIMA, S KINOSHITA, Y NISHIO, S NATSUKA, T KISHIMOTO
POLYFUNCTIONAL CYTOKINES : IL-6 AND LIF 167 47 - 67 1992
INTERLEUKIN-6 RECEPTOR AND A UNIQUE MECHANISM OF ITS SIGNAL TRANSDUCTION Reviewed
T TAGA, M HIBI, Y HIRATA, H YAWATA, S NATSUKA, K YASUKAWA, T TOTSUKA, K YAMASAKI, T HIRANO, T KISHIMOTO
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 54 713 - 722 1989
Interleukin 6 and its receptor
Toshio Hirano, Tetsuya Taga, Katsuhiko Yamasaki, Tadashi Matsuda, Sachiko Suematsu, Bo Tang, Yukihiko Hirata, Hideo Yawata, Masahiko Hibi, Shunji Natsuka, Shizuo Akira, Kazuyuki Yoshizaki, M. Kawano, Kenichi Yamamura, Tadamitsu Kishimoto
Recent Progress in Cytokine Research 265 - 285 1989
日本糖質学会奨励賞
2004.8
日本生化学会JB論文賞
2000.10
長束 俊治
Studies on glycopeptides containing a rare sugar 6-deoxyaltrose secreted from vertebrate embryos
Grant number:20K06539
2020.4 - 2023.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )
Development of methods for making glycan atlas, and data collection for database publishing
Grant number:15K06991
2015.4 - 2018.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Natsuka Shunji, SUZUKI Noriko
Grant amount:\5200000 ( Direct Cost: \4000000 、 Indirect Cost:\1200000 )
A preparing method for glycan atlas, which is a comprehensive database of glycan structure and expression level, was adapted to glycan molecules of various forms. Using this method, we succeeded in comprehensively analyzing the changes in glycan structure during the embryonic development of zebrafish, and some of these results have been published. On the other hand, we also succeeded to accumulate data on structure and expression level by analyzing glycan structure of glycoproteins and animal tissues and published on internet as 2 dimensional glycan maps. This study overcame the technical problems for producing of mice and human glycan atlases.
Study on glycans related to embryogenesis of vertebrates
Grant number:14580625
2002 - 2004
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
NATSUKA Shunji
Grant amount:\3000000 ( Direct Cost: \3000000 )
We have analyzed expression period and substrate specificity of zebrafish a(1,3)fucosyltransferase (zFT1). As a results, the zFT1 expressed specifically during segmentation period of embryogenesis, and could synthesize Lewis x structure, but not sialyl Lewis x or Lewis a structures. The zFT1 preferred to transfer fucose residue to inner N-acetyllactosamine unit rather than the terminal unit when tandem tri-N-acetyllactosamine oligosaccharide was used as a substrate. The results of in situ hybridization showed that the zFT1 expressed dispersedly at neural tube and around. We have investigated the structure of N-linked glycans expressed in zebrafish embryo. The expression of biantenna-complex-type glycans was remarkably increased from segmentation period. We searched also Lewis x-containing glycans, and found that the biantenna-complex-type glycans had one or two Lewis x structures at their non-reducing end. Those glycans expressed in segmentation period simultaneously with zFI1. We discovered unusual glycan structure that had additional Galb1-4 residue attached to N-acetyllactosamine structure, and also free oligosaccharides with similar structure. As a result of searching for core proteins of Lewis x containing biantenna-complex-type glycans, a glycoprotein with a molecular weight of 62,000 was found as a carrier protein from zebrafish embryos in segmentation period. We designated the glycoprotein as zGP62. Furthermore, we measured the zFT1 activity against Galb1-4Galb1-4GlcNAcb1-R structure, and zFT1 could not utilized that structure as a substrate. Thus it was speculated that the Galb1-4Galb1-4GlcNAcb1-R structure was synthesized by an unknown b1-4galactosyltransferase which could act on Lewis x structure.
神経細胞変性疾患に関わる蛋白質架橋酵素の構造と機能の研究
Grant number:12780453
2000 - 2001
System name:科学研究費助成事業
Research category:奨励研究(A)
Awarding organization:日本学術振興会
長束 俊治
Grant amount:\1900000 ( Direct Cost: \1900000 )
前年度までの研究によって、2種の組織型トランスグルタミナーゼのオープンリーディングフレームを含む遺伝子断片をヒト脳由来cDNAから単離することに成功している。この2種類の遺伝子を用いて、大腸菌の系でリコンビナントタンパク質の発現を試みた。前年度の研究によって、不溶型のリコンビナントタンパク質を大量に調製することに成功しているので、これを抗原に用いて、ヒト脳型トランスグルタミナーゼ特異的抗体の作製を行った。マウスを免疫した結果、ELISA分析により特異的な抗体を検出することができたので、現在、抗ヒト脳型トランスグルタミナーゼモノクローナル抗体産生ハイブリドーマ株の樹立を行っている。また一方、リコンビナントタンパク質の酵素化学的およびタンパク質化学的性質の詳細な解析を行うために、可溶化条件の検討を行った。その結果、インビトロにおいて、ほぼ定量的に可溶性リコンビナントタンパク質を得ることができたが、酵素比活性は自然型の酵素よりもかなり低かった。これは、可溶化はされたものの、自然型酵素のホールディングを再現できなかったためと考えられたので、各種シャペロンタンパク質を宿主大腸菌内で強制発現させることにより、リコンビナントタンパク質の可溶化と正しいホールディングの効率を上げることを試みた。その結果、従来の発現系よりも可溶性の比率を増加させ、また比活性も高いリコンビナントトランスグルタミナーゼを得ることに成功した。
胚に特異的な糖鎖を生合成するフコース転移酵素の遺伝子クローニングと発現部位の検索
Grant number:10780368
1998 - 1999
System name:科学研究費助成事業
Research category:奨励研究(A)
Awarding organization:日本学術振興会
長束 俊治
Grant amount:\2200000 ( Direct Cost: \2200000 )
前年度に遺伝子の単離を行った2種類のゼブラフィッシュフコース転移酵素の基質特異性を解析した。発現ベクターに組み込んだ遺伝子を培養細胞に導入し、酵素を発現させた後、紬胞を溶解して酵素元とした。基質として1型構造糖鎖、2型構造糖鎖、シアル酸の付いた2型構造糖鎖を用いたところ、2型構造糖鎖のみにフコースを転移した。この基質特異性は、脊椎動物の胚における存在が酵素学的な研究から指摘されているフコース転移酵素と一致している。前年度に示した本酵素遺伝子の胚における特異的な発現と合わせて、我々がクローニングした遺伝子は、以前より多くの糖鎖研究者によって探し求められてきた、胚特異的ルイスx糖鎖を生合成するフコース転移酵素であることが強く示唆された。胚特異的ルイスx糖鎖は、特に神経外胚葉に強く発現しており、神経系の発生に重要な役割を果たしていることが示唆されている。現在、本酵素遺伝子の詳細な発現部位の特定を目指して、in situハイブリダイゼーション法による分析を行っている。
次に、本酵素によって生合成されて胚細胞で発現している糖鎖の構造解析を目的として、ゼブラフィッシュ胚で特異的に発現しているフコシルオリゴ糖鎖の検索を行った。N-配糖体とO-配糖体をともにピリジルアミノ化による2次元マッピング法で展開し、フコシダーゼ消化と組み合わせてスクリーニングしたところ、O-配糖体の9グルコース単位の画分にフコース転移酵素の発現時期と一致して発現しているフコシルオリゴ糖鎖を確認できた。
ゼブラフィッシュの胚発生において特異的に発現する糖鎖の検索とその構造解析
Grant number:08780559
1996
System name:科学研究費助成事業
Research category:奨励研究(A)
Awarding organization:日本学術振興会
長束 俊治
Grant amount:\1100000 ( Direct Cost: \1100000 )
1.ゼブラフィッシュの受精卵を種々の時間培養して胚発生を進行させた後に、各発生段階の卵を調製した。この受精卵からヒドラジン分解とピリジルアミノ化法の組み合わせによって蛍光標識した糖蛋白質糖鎖を調製する方法を確立した。
2.受精後すぐから48時間後に孵化するまでの受精卵に存在する糖蛋白質糖鎖の構造解析を行ったところ、マンノース9個を持つオリゴマンノース型糖鎖が最も多く存在しており、その他にもマンノース7から8個やマンノース9個にグルコース1個を持つオリゴマンノース型糖鎖も相当量存在していることを発見した。しかしこれら受精卵中に比較的多量に含まれる糖鎖は、分析した胚発生時期には顕著な量的変化は示さなかった。一方、微量糖鎖は他種類観測された。それら微量糖鎖の中には、発生の進行とともに顕著な量的増減を示す物があった。
3.以上のように観測された糖鎖が受精卵中の、どの部位に由来するのかを解析するために、受精卵の微小解剖の方法を確立した。受精卵を被う卵膜を切り取って除いた後、卵黄と細胞を分離し、胚細胞を精製した。この精製した胚細胞を用いることにより、胚の分化発生に直接関わっている糖鎖の解析が可能となった。
ホスファチジルイノシトール・グリカンの糖鎖構造解析
Grant number:07858069
1995
System name:科学研究費助成事業
Research category:奨励研究(A)
Awarding organization:日本学術振興会
長束 俊治
Grant amount:\900000 ( Direct Cost: \900000 )
1.亜硝酸脱アミノ化とピリジルアミノ化の反応条件を設定するために、グルコサミンを用いて至適条件を検討した。50mM亜硝酸溶液中で25℃、16時間亜硝酸脱アミノ化し、次いで蒸発乾固の後、定法よりも穏和な条件でピリジルアミノ化を行い、40%の収率でグルコサミンから2,5‐アンヒドロマンノースを得る系を確立した。
2.ラット肝臓より、溶媒抽出によって脂質画分を調製し、前述の方法に従って亜硝酸による脱アミノ化を行い、目的のホスファチジルイノシトール・グリカン(PIG)から糖鎖部分を分離した。分離したオリゴ糖をピリジルアミノ化によって蛍光標識し、ゲル濾過によって過剰の試薬を除去した。こうしてクル-ドなPIG糖鎖画分を得た。還元末端糖分析の結果、ゲル濾過カラム体積の約100%に相当する部分に、2,5‐アンヒドロマンノースを還元末端にもつオリゴ糖が検出された。このオリゴ糖を精製するために、まずサイズが分画HPLCで展開したところ目的の糖鎖は3.1グルコース単位の位置に溶出されてきた。さらに精製を進めるために、逆相HPLCに供したところ、単一ピークにまで精製することができた。
3.得られたPIG由来ピリジルアミノ化オリゴ糖の構造解析を行った。酸加水分解して還元末端糖分析を行ったところ、2,5‐アンヒドロマンノースが検出されたので、脱アミノ化前の還元末端は、グルコサミンだと推定された。さらに糖水解酵素消化と糖組成分析を行い、糖鎖構造解析を行った。
基礎生物化学
生命科学のための基礎化学
生物分析化学
生体分子機能学
生化学
有機生物化学
分子生物学
生物化学
糖鎖生物学
生物学基礎B
インターンシップ特別実習b
インターンシップ特別実習a
生物学実習II
生物学実習I
糖鎖科学特論
理学スタディ・スキルズ
日本事情自然系A
生物学実験
生物学特論I
基礎生命科学セミナー
科学・技術と社会
総合力アクティブ・ラーニング
課題研究II(生物学)
自然科学基礎実験
生物学基礎実習b
課題研究I(生物学)
生物学総合演習
生物化学I(理)
理学スタディ・スキルズ
生物学基礎演習
生体分子機能学実習
生命科学への招待(生物学学習法)
生命・食料科学博士特定研究Ⅱ
生命・食料科学博士セミナーⅡ
基礎生命科学(博士)演習(中間発表)
基礎生物科学実習I
外国語論文解説・討論Ⅰ
生命・食料科学博士特定研究Ⅰ
生命・食料科学博士セミナーⅠ
生物学特論Ⅵ
生物化学I
文献詳読Ⅱ
研究発表演習(中間発表)
生命・食料科学セミナーBⅡ
生物学実習
生命科学のための基礎化学
課題研究I(生物学科)
生命・食料科学セミナーBⅠ
生命・食料科学特定研究BⅠ
生命・食料科学特定研究BⅡ
文献詳読Ⅰ
生物化学II
リサーチキャンプ
生物学実験 I
細胞生物学演習
自然科学総論Ⅳ
生物学特論II B
基礎生物化学
生物化学演習
生物学基礎B
糖鎖生物学
糖鎖科学特論
日本事情自然系A
課題研究II
生物化学実習
基礎生物科学実習II
生物化学
課題研究I