Updated on 2024/04/19

写真a

 
TAKATSUKA Hisakazu
 
Organization
Academic Assembly Institute of Medicine and Dentistry IGAKU KEIRETU Professor
Faculty of Medicine School of Medicine Professor
Graduate School of Medical and Dental Sciences Community Disease Control Community Preventive Medicine Professor
Title
Professor
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Degree

  • 博士(医学) ( 1998.3   新潟大学 )

Research Interests

  • マクロファージ

  • 法医病理学

  • Macrophage

  • Forensic pathology

Research Areas

  • Life Science / Applied biochemistry

Research History (researchmap)

  • Former Institution / Organization University of Fukui Faculty of Medical Sciences School of Medicine Faculty of Medicine   Professor

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  • Niigata University Graduate School of Medical and Dental Sciences, Course of Community Disease Control

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Research History

  • Niigata University   Graduate School of Medical and Dental Sciences Community Disease Control Community Preventive Medicine   Professor

    2015.5

  • Niigata University   Faculty of Medicine School of Medicine   Professor

    2015.5

  • Niigata University   Faculty of Medicine School of Medicine   Associate Professor

    2009.11 - 2015.5

  • Niigata University   Graduate School of Medical and Dental Sciences Community Disease Control Community Preventive Medicine   Associate Professor

    2009.11 - 2015.5

Education

  • Toyama Medical and Pharmaceutical University   Faculty of Medicine

    - 1992.3

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    Country: Japan

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Professional Memberships

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Books

  • マウス腎年齢依存性発現分子M-LPの細胞内局在と生理的機能(共著)

    DNA多型  2003 

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  • DXS10011型変異型3例の構造(共著)

    DNA多型  2003 

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  • Structures of three mutations in DXS10011 locus.(jointly worked)

    DNA Polymorphism  2003 

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  • Subcellular localization and physiological function of M-LP, the age dependently expressed molecule in mouse kidney.(jointly worked)

    DNA Polymorphism  2003 

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  • Y染色体上の新規多型性STR5種の同定とハプロタイプ解析(共著)

    DNA多型  2002 

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  • Identification and haplotype analysis of 5 novel polymorphic Y-STRs.(jointly worked)

    DNA Polymorphism  2002 

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  • 肝再生におけるKupffer細胞の役割(共著)

    肝類洞壁細胞研究の進歩  1999 

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  • 大理石病マウス(op/op)におけるLPS肝障害(共著)

    肝類洞壁細胞研究の進歩  1999 

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  • The role of Kupffer cells in liveer regeneration.(jointly worked)

    Progress in Hepatic Sinusoidal Cell Studies  1999 

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  • LPS-induced liver injury in osteopetrotic (op/op) mice.(jointly worked)

    Progress in Hepatic sinusoidal Cell Studies  1999 

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  • BCG誘発肝肉芽腫形成モデルにおけるスカベンジャー受容体とマクロファージコロニー刺激因子の発現(共著)

    肝類洞壁細胞研究の進歩  1997 

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  • Scavenger receptor and macrophage colony-stimulating favtor expressions in the BCG-induced hepatic granuloma. (jointly worked)

    Progress in Hepatic Sinusoidal Cell Studies  1997 

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MISC

  • M-LP, Mpv17-like protein, has a peroxisomal membrane targeting signal comprising a transmembrane domain and a positively charged loop and up-regulates expression of the manganese superoxide dismutase gene

    R Iida, T Yasuda, E Tsubota, H Takatsuka, M Masuyama, T Matsuki, K Kishi

    JOURNAL OF BIOLOGICAL CHEMISTRY   278 ( 8 )   6301 - 6306   2003.2

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    Language:English   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    M-LP (Mpv17-like protein) has been identified as a new protein that has high sequence homology with Mpv17 protein, a peroxisomal membrane protein involved in the development of early onset glomerulosclerosis. In this study, we verified the peroxisomal localization of M-LP by performing dual-color confocal analysis of COS-7 cells cotransfected with green fluorescent protein-tagged M-LP and DsRED2-PTS1, a red fluorescent peroxisomal marker. To characterize the peroxisomal membrane targeting signal, we examined the intracellular localizations of several green fluorescent protein-tagged deletion mutants and demonstrated that, of the three transmembrane segments predicted, the first near the NH2 terminus and NH2-terminal half of the following loop region, which is abundant in positively charged amino acids, were necessary and sufficient for peroxisomal targeting. To elucidate the function of M-LP, we examined the activities of several enzymes involved in reactive oxygen species metabolism in COS-7 cells and found that transfection with M-LP increased the superoxide dismutase activity significantly. Quantitative real-time PCR analysis revealed that the manganese SOD (SOD2) mRNA level of COS-7 cells transfected with M-LP was elevated. These results indicate that M-LP participates in reactive oxygen species metabolism.

    DOI: 10.1074/jbc.M210886200

    Web of Science

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  • M-LP, Mpv17-like protein, has a peroxisomal membrane targeting signal comprising a transmembrane domain and a positively charged loop and up-regulates expression of the manganese superoxide dismutase gene

    R Iida, T Yasuda, E Tsubota, H Takatsuka, M Masuyama, T Matsuki, K Kishi

    JOURNAL OF BIOLOGICAL CHEMISTRY   278 ( 8 )   6301 - 6306   2003.2

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    Language:English   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    M-LP (Mpv17-like protein) has been identified as a new protein that has high sequence homology with Mpv17 protein, a peroxisomal membrane protein involved in the development of early onset glomerulosclerosis. In this study, we verified the peroxisomal localization of M-LP by performing dual-color confocal analysis of COS-7 cells cotransfected with green fluorescent protein-tagged M-LP and DsRED2-PTS1, a red fluorescent peroxisomal marker. To characterize the peroxisomal membrane targeting signal, we examined the intracellular localizations of several green fluorescent protein-tagged deletion mutants and demonstrated that, of the three transmembrane segments predicted, the first near the NH2 terminus and NH2-terminal half of the following loop region, which is abundant in positively charged amino acids, were necessary and sufficient for peroxisomal targeting. To elucidate the function of M-LP, we examined the activities of several enzymes involved in reactive oxygen species metabolism in COS-7 cells and found that transfection with M-LP increased the superoxide dismutase activity significantly. Quantitative real-time PCR analysis revealed that the manganese SOD (SOD2) mRNA level of COS-7 cells transfected with M-LP was elevated. These results indicate that M-LP participates in reactive oxygen species metabolism.

    DOI: 10.1074/jbc.M210886200

    Web of Science

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  • A Novel Multiplex PCR System Consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445.(jointly worked)

    Journal of Forensic Science   Vol. 48(5), 1088-1090   2003

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  • A Novel Multiplex PCR System Consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445.(共著)

    Journal of Forensic Science   Vol. 48(5), 1088-1090   2003

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  • 屋内で死後1年以上経過して発見された陳旧死体10例の検討(共著)

    法医学の実際と研究   45巻 167-174頁   2002

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  • Ten cases of cadavers found indoors more than one year.(jointly worked)

    The Research and Practice in Forensic Medicine   Vol. 45, 167-174   2002

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  • CpG DNA-induced IFN-α production involves p38 MAPK-dependent STST1 phosphorylation in human plasmacytoid dendric cell precursors.(jointly worked)

    Journal of Leukocyte Biology   Vol.72 (5), 1011-1019   2002

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  • Five age-dependently expressed genes in mouse brain revealed by the fluorescence differential display-PCR technique.(jointly worked)

    Experimental Gerontology   Vol.37(8-9), 1121-1126   2002

  • CpG DNA-induced IFN-α production involves p38 MAPK-dependent STST1 phosphorylation in human plasmacytoid dendric cell precursors.(共著)

    Journal of Leukocyte Biology   Vol.72 (5), 1011-1019   2002

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  • Five age-dependently expressed genes in mouse brain revealed by the fluorescence differential display-PCR technique.(共著)

    Experimental Gerontology   Vol.37(8-9), 1121-1126   2002

  • Maturation of marginal zone and follicular B cells requires BAFF and is independent of BCMA.(共著)

    Journal of Experimental Medicine   Vol.194(11), 1691-1697   2001

  • Maturation of marginal zone and follicular B cells requires BAFF and is independent of BCMA.(jointly worked)

    Journal of Experimental Medicine   Vol.194(11), 1691-1697   2001

  • Granuloma formation in scavenger receptor-deficient mice in response to BCG.(共著)

    Cells of the Hepatic Sinusoid   Vol.7, 243-244   1999

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  • Gene transduction into the liver - Present and perspective - Immunomodulation gene therapy for hepatic micrometastasis.(jointly worked)

    Cells of the Hepatic Sinusoid   Vol.7, 272-277   1999

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  • Granuloma formation in scavenger receptor-deficient mice in response to BCG.(jointly worked)

    Cells of the Hepatic Sinusoid   Vol.7, 243-244   1999

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  • Increased Susceptibility of Thymocytes to Apoptosis in Mice Lacking AIM, a Novel Murine Macrophage-derived Soluble Factor Belonging to the Scavenger Receptor Cysteine-rich Domain Superfamily.(共著)

    Journal of Experimental Medicine   Vol.189(2), 413-422   1999

  • Increased Susceptibility of Thymocytes to Apoptosis in Mice Lacking AIM, a Novel Murine Macrophage-derived Soluble Factor Belonging to the Scavenger Receptor Cysteine-rich Domain Superfamily.(jointly worked)

    Journal of Experimental Medicine   Vol.189(2), 413-422   1999

  • The role of Kupffer cells and regulation of neutrophil migration into the liver by macrophage inflammatory protein-2 in primary listeriosis in mice.(共著)

    Pathology International   Vol.49(6), 519-532   1999

  • The role of Kupffer cells and regulation of neutrophil migration into the liver by macrophage inflammatory protein-2 in primary listeriosis in mice.(jointly worked)

    Pathology International   Vol.49(6), 519-532   1999

  • Roles of Macrophage Receptor with Collagenous Structure (MARCO) in Host Defense and Heterogeneity of Splenic Marginal Zone Macrophages.(共著)

    Archives of Histology and Cytology   Vol.62(1), 83-95   1999

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  • Roles of Macrophage Receptor with Collagenous Structure (MARCO) in Host Defense and Heterogeneity of Splenic Marginal Zone Macrophages.(jointly worked)

    Archives of Histology and Cytology   Vol.62(1), 83-95   1999

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  • Gene transduction into the liver - Present and perspective - Immunomodulation gene therapy for hepatic micrometastasis.(共著)

    Cells of the Hepatic Sinusoid   Vol.7, 272-277   1999

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  • Expression of Macrophage Colony-stimulating Factor, Scavenger Receptors, and Macrophage Proliferation in Pregnant Uterus of Mice.(jointly worked)

    Archives of Histology and Cytology   Vol.61(5), 383-393   1998

  • Bone remodeling and macrophage differentiation in aged osteopetrosis (op) mutant mice defective in the production of macrophage colony-stimulating factor.(jointly worked)

    Journal of Submicroscopic Cytology and Pathology   Vol.30(2), 239-247   1998

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  • Bone remodeling and macrophage differentiation in aged osteopetrosis (op) mutant mice defective in the production of macrophage colony-stimulating factor.(共著)

    Journal of Submicroscopic Cytology and Pathology   Vol.30(2), 239-247   1998

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  • Expression of Macrophage Colony-stimulating Factor, Scavenger Receptors, and Macrophage Proliferation in Pregnant Uterus of Mice.(共著)

    Archives of Histology and Cytology   Vol.61(5), 383-393   1998

  • Expression of macrophage colony-stimulating factor and its receptor in hepatic granulomas of Kupffer-cell-depleted mice.(共著)

    American Journal of Pathology   Vol.150(6), 2047-2060   1997

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  • Macrophage differentiation and expression of macrophage colony stimulating factor in murine mikly spots and omentum after macrophage elimination.(jointly worked)

    Journal of Leukocyte Biology   Vol.61(4), 436-444   1997

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  • Bone reconstruction and crystal storage in bone marrow macrophages of aged osteopetrosis (op) mice.(jointly worked)

    Dendritic Cells   Vol.7, 43-46   1997

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  • Expression of macrophage colony-stimulating factor and its receptor in hepatic granulomas of Kupffer-cell-depleted mice.(jointly worked)

    American Journal of Pathology   Vol.150(6), 2047-2060   1997

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  • Bone reconstruction and crystal storage in bone marrow macrophages of aged osteopetrosis (op) mice.(共著)

    Dendritic Cells   Vol.7, 43-46   1997

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  • Macrophage differentiation and expression of macrophage colony stimulating factor in murine mikly spots and omentum after macrophage elimination.(共著)

    Journal of Leukocyte Biology   Vol.61(4), 436-444   1997

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  • Liposome-encapsulated dichloromethylene diphosphonate induces macrophage apoptosis in vivo and in vitro.(共著)

    Journal of Leukocyte Biology   Vol.60(3), 337-344   1996

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  • Expression of Scavenger Receptors in macrophages and Endothelial Cells.(jointly worked)

    Niigata Medical Journal   Vol. 110(6), 216-220   1996

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  • Macrophage Differentiation in Osteopetrotic mice (op/op) Defective in the Production of Macrophage Colony-Stimulating Factor (M-CSF/CSF-1).(jointly worked)

    Niigata Medical Journal   Vol. 110(1), 1-12   1996

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  • Liposome-encapsulated dichloromethylene diphosphonate induces macrophage apoptosis in vivo and in vitro.(jointly worked)

    Journal of Leukocyte Biology   Vol.60(3), 337-344   1996

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  • Osteopetrotic (op/op) Mice: An animal model for investigating the biology of colony-stimulating factor-1 (CSF-1/M-CSF).(jointly worked)

    Acta Medica et Biologica   Vol.44(1), 1-11   1996

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  • Macrophage Differentiation in Osteopetrotic Mice (op/op) Defective inthe Production of Macrophage Colony-Stimulating Factor (M-CSF/CSF-1)

    Makoto NAITO, Hiroyuki USUDA, Hajime UMEZU

    Niigata medical journal   110巻1号 1-12項 ( 1 )   1 - 12   1996

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    Language:Japanese   Publisher:Niigata University  

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    Other Link: http://search.jamas.or.jp/link/ui/1996229042

  • Osteopetrotic (op/op) Mice: An animal model for investigating the biology of colony-stimulating factor-1 (CSF-1/M-CSF).(共著)

    Acta Medica et Biologica   Vol.44(1), 1-11   1996

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  • マクロファージと内皮細胞におけるスカベンジャー受容体の発現(共著)

    新潟医学会雑誌   110巻6号 216-220項   1996

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Works

  • 感染とトールライクレセプター

    2001

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  • Infection and toll-like receptors

    2001

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Research Projects

  • 熱中症の病態に関する研究

    2003

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    Grant type:Competitive

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  • Experimental heatstroke model

    2003

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    Grant type:Competitive

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  • マクロファージの機能に関する研究

    2001

    System name:共同研究

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    Grant type:Competitive

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  • Macrophage and infection

    2001

    System name:Cooperative Research

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    Grant type:Competitive

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Teaching Experience

  • 医学序説 I

    2022
    Institution name:新潟大学

  • 歯科法医学

    2021
    Institution name:新潟大学

  • 医学論文を読む(ジャーナルクラブ)A

    2021
    Institution name:新潟大学

  • 医学序説 II

    2021
    Institution name:新潟大学

  • 医学序説 I

    2020
    Institution name:新潟大学

  • 特別授業 歯科法医学

    2018
    -
    2020
    Institution name:新潟大学

  • 特殊講義(法医学Ⅰ)

    2016
    Institution name:新潟大学

  • 特殊講義(法医学Ⅱ)

    2016
    Institution name:新潟大学

  • 法医学

    2015
    Institution name:新潟大学

  • 医療と法

    2014
    Institution name:新潟大学

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