Faculty Profiles - SAKAI Tatsuya

写真a

SAKAI Tatsuya

Organization

Academic Assembly Institute of Science and Technology CHIKYU SEIBUTSU KAGAKU KEIRETU Professor
Faculty of Science Department of Science Professor

External link

Degree

Ph.D ( 1997.3 The University of Tokyo )

Research Areas

Life Science / Plant molecular biology and physiology

Research History (researchmap)

Niigata University Faculty of Science Department of Biology Bioregulatory Science Professor

2014.4

　 More details

researchmap
Niigata University Headquarters for Strategy and Planning Office for Promoting the Cultivation of Young Researchers Associate Professor

2010.2 - 2014.3

　 More details

researchmap
Japan Science and Technology Agency

2007.10 - 2011.3

　 More details

researchmap
RIKEN

2000.10 - 2010.1

　 More details

researchmap
日本学術振興会特別研究員（PD）

1998.4 - 2000.9

　 More details

researchmap

Research History

Niigata University Faculty of Science Department of Science Professor

2017.4
Niigata University Abolition organization Bioregulatory Science Professor

2014.4 - 2017.3
Niigata University Headquarters for Strategy and Planning Office for Promoting the Cultivation of Young Researchers Associate Professor

2010.2 - 2014.3

Education

The University of Tokyo Graduate School, Division of Science Plant Biology

- 1997.3

　 More details

Country： Japan

researchmap

Papers

Phototropin 2 mediates daily cold priming to promote light responses in Arabidopsis

Minoru Noguchi, Issei Keino, Hitomi Takahashi, Shota Yamauchi, Mami Fujisawa, Ken Haga, Tatsuya Sakai, Atsushi Takemiya, Yutaka Kodama

Journal of Experimental Botany 2025.1

　More details

Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

Abstract

Organisms adapt to predictable environmental changes via a biological mechanism called priming. Phototropin (phot) is a plant-specific blue light photoreceptor that mediates daily light-induced responses, such as chloroplast relocation, stomatal opening, and phototropism, to optimize photosynthesis. Phot also functions as a thermosensor for chloroplast relocation that may sense daily temperature decreases at night, thereby modulating light-induced responses at dawn; however, this hypothesis has not yet been fully explored. Here, we reveal that phot mediates daily cold priming to promote stomatal opening and phototropism in Arabidopsis (Arabidopsis thaliana) under dawn-mimicking conditions. A cold pretreatment in the dark enhanced subsequent blue light–induced stomatal opening and phototropism at normal temperatures, suggesting that daily cold priming is involved in these physiological responses. Arabidopsis has two phot proteins (phot1 and phot2), and we show that phot2 clearly mediates the cold priming of stomatal opening and phototropism. Cold priming appears to be based on phot-mediated thermosensing just before dawn, which plants use to optimize their light-induced responses in anticipation of dawn.

DOI： 10.1093/jxb/eraf040

researchmap
Protein phosphatase PP2C19 controls hypocotyl phototropism through the phosphorylation modification of NONPHOTOTROPIC HYPOCOTYL3 in Arabidopsis Reviewed

Tatsuya Sakai, Ken Haga, Taro Kimura, Keita Kawaura

Plant And Cell Physiology 66 ( 1 ) 23 - 35 2024.11

　More details

Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

Abstract

Plants exhibit shoot growth in the direction of the light source to facilitate photosynthesis, known as positive phototropism. In Arabidopsis hypocotyl phototropism, it is thought that a gradient of the signal intensity of the blue light (BL) photoreceptor phototropin1 (phot1) between the light-irradiated and shaded sides leads to the differential growth of hypocotyls. The intensity of phot1 signal is regulated not only by the protein kinase activity of phot1 but also by the phosphorylation status of the NONPHOTOTROPIC HYPOCOTYL3 (NPH3) protein, which has a dark form and a BL form of the phosphorylation modification. Previous studies have shown that phot1 drives the forward reaction from the dark form to the BL form of NPH3. However, the molecular mechanism underlying the reverse reaction remains unknown. Here, we show that protein phosphatase PP2C19 controls the reverse reaction that converts the BL form of NPH3 to the dark form of NPH3. The PP2C19 protein possesses the protein phosphatase type 2C (PP2C) domain, two cyclic nucleoside monophosphate (cNMP)-binding domains, and the protein kinase domain. Similar to phot1 and NPH3, PP2C19 localizes to the plasma membrane, and its PP2C domain is necessary and sufficient for PP2C19 function in hypocotyl phototropism. The pp2c19 mutants show abnormalities in second positive hypocotyl phototropism with a delay in the reverse reaction of NPH3 phosphorylation modification. The present study suggests that continuous BL irradiation induces an equilibrium state of the reversible reaction of NPH3 phosphorylation, which acts as a phot1 signaling gradient with phot1 kinase activity to induce the second positive phototropism.

DOI： 10.1093/pcp/pcae141

researchmap

Other Link： https://academic.oup.com/pcp/article-pdf/66/1/23/60876541/pcae141.pdf
Photosensory adaptation mechanisms in hypocotyl phototropism: how plants recognize the direction of a light source Reviewed

Ken Haga, Tatsuya Sakai

Journal of Experimental Botany 2023.1

　More details

Authorship：Corresponding author Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

Abstract

Plants recognize the direction of a light source and exhibit phototropic responses. Physiological studies have predicted that differences in the light intensity received by the cells on the irradiated and shaded sides of a coleoptile or hypocotyl cause differences in the amounts of photoproduct. This hypothetical photoproduct appears to regulate a signaling pathway that controls cell elongation in which cells under lower light intensity elongate more than those under higher light intensity. This results in a bending growth toward a light source and has been proposed as the photoproduct-gradient model of phototropism. In this review, we summarize recent findings on the photosensory adaptation mechanisms involving a blue-light photoreceptor, phototropin1 (phot1), ROOT PHOTOTROPISM2, NONPHOTOTROPIC HYPOCOTYL3 (NPH3), and another photoreceptor family, the phytochromes. The current evidence demonstrates that, in addition to the transition of the phot1–NPH3 photoreceptor complexes to their active state, the presence of a certain population of the phot1–NPH3 complexes showing a steady state, even in a light environment, is essential for recognition of the light source direction in phototropism. This is consistent with the photoproduct-gradient model, and a dissociation state of the phot1–NPH3 complex would be considered an entity of the hypothetical photoproduct in this model.

DOI： 10.1093/jxb/erad015

researchmap
The phosphorylation status of NONPHOTOTROPIC HYPOCOTYL3 affects phot2-dependent phototropism in Arabidopsis Reviewed

Taro Kimura, Ken Haga, Tatsuya Sakai

Plant Signaling & Behavior 17 ( 1 ) 2022.1

　More details

Authorship：Corresponding author Publishing type：Research paper (scientific journal) Publisher：Informa UK Limited

DOI： 10.1080/15592324.2022.2027138

researchmap
Intragenic complementation at the Lotus japonicus CELLULOSE SYNTHASE-LIKE D1 locus rescues root hair defects. Reviewed International journal

Bogumil Karas, Loretta Ross, Mara Novero, Lisa Amyot, Sayaka Inada, Michiharu Nakano, Tatsuya Sakai, Shusei Sato, Jeremy D Murray, Paola Bonfante, Krzysztof Szczyglowski

Plant Physiology 186 2037 - 2050 2021.5

　More details

Language：English Publishing type：Research paper (scientific journal)

Root hair cells form the primary interface of plants with the soil environment, playing key roles in nutrient uptake and plant defense. In legumes, they are typically the first cells to become infected by nitrogen-fixing soil bacteria during root nodule symbiosis. Here, we report a role for the CELLULOSE SYNTHASE-LIKE D1 (CSLD1) gene in root hair development in the legume species Lotus japonicus. CSLD1 belongs to the cellulose synthase protein family that includes cellulose synthases and cellulose synthase-like proteins, the latter thought to be involved in the biosynthesis of hemicellulose. We describe 11 Ljcsld1 mutant alleles that impose either short (Ljcsld1-1) or variable (Ljcsld1-2 to 11) root hair length phenotypes. Examination of Ljcsld1-1 and one variable-length root hair mutant, Ljcsld1-6, revealed increased root hair cell wall thickness, which in Ljcsld1-1 was significantly more pronounced and also associated with a strong defect in root nodule symbiosis. Lotus japonicus plants heterozygous for Ljcsld1-1 exhibited intermediate root hair lengths, suggesting incomplete dominance. Intragenic complementation was observed between alleles with mutations in different CSLD1 domains, suggesting CSLD1 function is modular and that the protein may operate as a homodimer or multimer during root hair development.

DOI： 10.1093/plphys/kiab204

PubMed

researchmap
The Phosphorylation Status of NPH3 Affects Photosensory Adaptation During the Phototropic Response Reviewed

Taro Kimura, Ken Haga, Yuko Nomura, Takumi Higaki, Hirofumi Nakagami, Tatsuya Sakai

Plant Physiology 187 ( 2 ) 981 - 995 2020.12

　More details

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press ({OUP})

<jats:title>Abstract</jats:title>
<jats:p>Photosensory adaptation, which can be classified as sensor or effector adaptation, optimizes the light sensing of living organisms by tuning their sensitivity to changing light conditions. During the phototropic response in Arabidopsis (Arabidopsis thaliana), the light-dependent expression controls of blue-light (BL) photoreceptor phototropin 1 (phot1) and its modulator ROOT PHOTOTROPISM2 (RPT2) are known as the molecular mechanisms underlying sensor adaptation. However, little is known about effector adaption in plant phototropism. Here, we show that control of the phosphorylation status of NONPHOTOTROPIC HYPOCOTYL3 (NPH3) leads to effector adaptation in hypocotyl phototropism. We generated unphosphorable and phosphomimetic NPH3 proteins on seven phosphorylation sites in the etiolated seedlings of Arabidopsis. Unphosphorable NPH3 showed a shortening of its retention time in the cytosol and caused an inability to adapt to very low fluence rates of BL (∼10−5 µmol m−2 s−1) during the phototropic response. In contrast, the phosphomimetic NPH3 proteins had a lengthened retention time in the cytosol and could not enable the adaptation to BL at fluence rates of 10−3 µmol m−2 s−1 or more. Our results indicate that the activation level of phot1 and the corresponding phosphorylation level of NPH3 determine the dissociation rate and the reassociation rate of NPH3 on the plasma membrane, respectively. These mechanisms may moderately maintain the active state of phot1 signaling across a broad range of BL intensities and contribute to the photosensory adaptation of phot1 signaling during the phototropic response in hypocotyls.</jats:p>

DOI： 10.1101/2020.12.01.407205

researchmap
Arabidopsis ROOT PHOTOTROPISM2 Is a Light-Dependent Dynamic Modulator of Phototropin1 Reviewed

Tatsuya Sakai

The Plant Cell 32 2004 - 2019 2020

　More details

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1105/TPC.19.00926

Web of Science

researchmap
Low-fluence blue light-induced phosphorylation of Zmphot1 mediates the first positive phototropism Reviewed International journal

Hiromi Suzuki, Tomokazu Koshiba, Chiharu Fujita, Yoshio Yamauchi, Taro Kimura, Toshiaki Isobe, Tatsuya Sakai, Masato Taoka, Takashi Okamoto

Journal of Experimental Botany 70 ( 20 ) 5929 - 5941 2019

　More details

Language：English Publishing type：Research paper (scientific journal)

Phototropin1 (phot1) perceives low- to high-fluence blue light stimuli and mediates both the first and second positive phototropisms. High-fluence blue light is known to induce autophosphorylation of phot1, leading to the second positive phototropism. However, the phosphorylation status of phot1 by low-fluence blue light that induces the first positive phototropism had not been observed. Here, we conducted a phosphoproteomic analysis of maize coleoptiles to investigate the fluence-dependent phosphorylation status of Zmphot1. High-fluence blue light induced phosphorylation of Zmphot1 at several sites. Notably, low-fluence blue light significantly increased the phosphorylation level of Ser291 in Zmphot1. Furthermore, Ser291-phosphorylated and Ser369Ser376-diphosphorylated peptides were found to be more abundant in the low-fluence blue light-irradiated sides than in the shaded sides of coleoptiles. The roles of these phosphorylation events in phototropism were explored by heterologous expression of ZmPHOT1 in the Arabidopsis thaliana phot1phot2 mutant. The first positive phototropism was restored in wild-type ZmPHOT1-expressing plants; however, plants expressing S291A-ZmPHOT1 or S369AS376A-ZmPHOT1 showed significantly reduced complementation rates. All transgenic plants tested in this study exhibited a normal second positive phototropism. These findings provide the first indication that low-fluence blue light induces phosphorylation of Zmphot1 and that this induced phosphorylation is crucial for the first positive phototropism.

DOI： 10.1093/JXB/ERZ344

PubMed

researchmap
Deetiolation Enhances Phototropism by Modulating NON-PHOTOTROPIC HYPOCOTYL3 Phosphorylation Status Reviewed

Tatsuya Sakai

Plant Physiology 180 ( 2 ) 1119 - 1131 2019

　More details

Publishing type：Research paper (scientific journal)

DOI： 10.1104/PP.19.00206

Web of Science

researchmap
Determination of Auxin Flow During Phototropic Responses Using Fluorescent Auxin Analogs. Reviewed

Sakai T

Methods in molecular biology (Clifton, N.J.) 1924 157 - 163 2019

　More details

Authorship：Lead author,　Corresponding author

DOI： 10.1007/978-1-4939-9015-3_12

PubMed

researchmap
Roles of AGCVIII Kinases in the Hypocotyl Phototropism of Arabidopsis Seedlings Reviewed

Ken Haga, Lena Frank, Taro Kimura, Claus Schwechheimer, Tatsuya Sakai

Plant and Cell Physiology 59 ( 5 ) 1060 - 1071 2018.5

　More details

Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press

DOI： 10.1093/pcp/pcy048

Scopus

PubMed

researchmap
Asymmetric Auxin Distribution is Not Required to Establish Root Phototropism in Arabidopsis Reviewed

Kimura, T., Haga, K., Shimizu-Mitao, Y., Takebayashi, Y., Kasahara, H., Hayashi, K., Kakimoto, T., Sakai, T.

Plant and Cell Physiology 59 ( 4 ) 823 - 835 2018.4

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pcy018

Web of Science

researchmap
Involvement of PP6-type protein phosphatase in hypocotyl phototropism in Arabidopsis seedlings Reviewed

Tatsuya Sakai

Plant Signaling and Behavior 13 ( 11 ) e1536631 2018

　More details

Publishing type：Research paper (scientific journal)

DOI： 10.1080/15592324.2018.1536631

Web of Science

PubMed

researchmap
Yucasin DF, a potent and persistent inhibitor of auxin biosynthesis in plants Reviewed

Tsugafune, Shinichi, Mashiguchi, Kiyoshi, Fukui, Kosuke, Takebayashi, Yumiko, Nishimura, Takeshi, Sakai, Tatsuya, Shimada, Yukihisa, Kasahara, Hiroyuki, Koshiba, Tomokazu, Hayashi, Ken-ichiro

SCIENTIFIC REPORTS 7 2017.10

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1038/s41598-017-14332-w

Web of Science

researchmap
The ARM Domain of ARMADILLO-REPEAT KINESIN 1 is Not Required for Microtubule Catastrophe But Can Negatively Regulate NIMA-RELATED KINASE 6 in Arabidopsis thaliana Reviewed

Ryan C. Eng, Laryssa S. Halat, Samuel J. Livingston, Tatsuya Sakai, Hiroyasu Motose, Geoffrey O. Wasteneys

PLANT AND CELL PHYSIOLOGY 58 ( 8 ) 1350 - 1363 2017.8

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pcx070

Web of Science

researchmap
Red/Far Red Light Controls Arbuscular Mycorrhizal Colonization via Jasmonic Acid and Strigolactone Signaling Reviewed

Maki Nagata, Naoya Yamamoto, Tamaki Shigeyama, Yohei Terasawa, Toyoaki Anai, Tatsuya Sakai, Sayaka Inada, Susumu Arima, Masatsugu Hashiguchi, Ryo Akashi, Hideyuki Nakayama, Daisuke Ueno, Ann M. Hirsch, Akihiro Suzuki

PLANT AND CELL PHYSIOLOGY 56 ( 11 ) 2100 - 2109 2015.11

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pcv135

Web of Science

researchmap
Distinct Characteristics of Indole-3-Acetic Acid and Phenylacetic Acid, Two Common Auxins in Plants Reviewed

Satoko Sugawara, Kiyoshi Mashiguchi, Keita Tanaka, Shojiro Hishiyama, Tatsuya Sakai, Kousuke Hanada, Kaori Kinoshita-Tsujimura, Hong Yu, Xinhua Dai, Yumiko Takebayashi, Noriko Takeda-Kamiya, Tatsuo Kakimoto, Hiroshi Kawaide, Masahiro Natsume, Mark Estelle, Yunde Zhao, Ken-ichiro Hayashi, Yuji Kamiya, Hiroyuki Kasahara

PLANT AND CELL PHYSIOLOGY 56 ( 8 ) 1641 - 1654 2015.8

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pcv088

Web of Science

researchmap
PINOID functions in root phototropism as a negative regulator Reviewed

Ken Haga, Tatsuya Sakai

PLANT SIGNALING & BEHAVIOR 10 ( 5 ) 2015.5

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1080/15592324.2014.998545

Web of Science

researchmap
Arabidopsis ROOT PHOTOTROPISM2 Contributes to the Adaptation to High-Intensity Light in Phototropic Responses Reviewed

Ken Haga, Tomoko Tsuchida-Mayama, Mizuki Yamada, Tatsuya Sakai

PLANT CELL 27 ( 4 ) 1098 - 1112 2015.4

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1105/tpc.15.00178

Web of Science

researchmap
Yucasin is a potent inhibitor of YUCCA, a key enzyme in auxin biosynthesis (vol 77, pg 352, 2014) Reviewed

Nishimura T, Hayashi K- I, Suzuki H, Gyohda A, Takaoka C, Sakaguchi Y, Matsumoto S, Kasahara H, Sakai T, Kato J- I, Kamiya Y, Koshiba T

PLANT JOURNAL 81 ( 4 ) 649 2015.2

　More details

DOI： 10.1111/tpj.12552

Web of Science

researchmap
PINOID AGC Kinases Are Necessary for Phytochrome-Mediated Enhancement of Hypocotyl Phototropism in Arabidopsis Reviewed

Ken Haga, Ken-ichiro Hayashi, Tatsuya Sakai

PLANT PHYSIOLOGY 166 ( 3 ) 1535 - + 2014.11

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1104/pp.114.244434

Web of Science

researchmap
The Phototropic Response is Locally Regulated Within the Topmost Light-Responsive Region of the Arabidopsis thaliana Seedling Reviewed

Kazuhiko Yamamoto, Tomomi Suzuki, Yusuke Aihara, Ken Haga, Tatsuya Sakai, Akira Nagatani

PLANT AND CELL PHYSIOLOGY 55 ( 3 ) 497 - 506 2014.3

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pct184

Web of Science

researchmap
Yucasin is a potent inhibitor of YUCCA, a key enzyme in auxin biosynthesis Reviewed

Takeshi Nishimura, Ken-ichiro Hayashi, Hiromi Suzuki, Atsuko Gyohda, Chihiro Takaoka, Yusuke Sakaguchi, Sachiko Matsumoto, Hiroyuki Kasahara, Tatsuya Sakai, Jun-ichi Kato, Yuji Kamiya, Tomokazu Koshiba

PLANT JOURNAL 77 ( 3 ) 352 - 366 2014.2

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1111/tpj.12399

Web of Science

researchmap
Function of ABCBs in Light Signaling

Tatsuya Sakai

Transporters and Pumps in Plant Signaling 2014

　More details

Publishing type：Research paper (scientific journal)

DOI： 10.1007/978-3-319-06511-3_16

Web of Science

researchmap
Mycorrhizal infection is controlled by the light quality and quantity in higher plants

SUZUKI Akihiro, NAGATA Maki, YAMAMOTO Naoya, SHIGEYAMA Tamaki, ANAI Toyoaki, ARIMA Susumu, SAKAI Tatsuya, HIRSCH Ann M

82 236 - 237 2013.3

　More details

Language：Japanese

CiNii Article

CiNii Books

researchmap
Differential roles of auxin efflux carrier PIN proteins in hypocotyl phototropism of etiolated Arabidopsis seedlings depend on the direction of light stimulus Reviewed

Ken Haga, Tatsuya Sakai

Plant Signaling and Behavior 8 ( 1 ) 50 - 52 2013.1

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.4161/psb.22556

Scopus

PubMed

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Role of RPT2 in Leaf Positioning and Flattening and a Possible Inhibition of phot2 Signaling by phot1 Reviewed

Akiko Harada, Atsushi Takemiya, Shin-ichiro Inoue, Tatsuya Sakai, Ken-ichiro Shimazaki

PLANT AND CELL PHYSIOLOGY 54 ( 1 ) 36 - 47 2013.1

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pcs094

Web of Science

researchmap
PIN Auxin Efflux Carriers Are Necessary for Pulse-Induced But Not Continuous Light-Induced Phototropism in Arabidopsis Reviewed

Ken Haga, Tatsuya Sakai

PLANT PHYSIOLOGY 160 ( 2 ) 763 - 776 2012.10

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1104/pp.112.202432

Web of Science

researchmap
Molecular Genetic Analysis of Phototropism in Arabidopsis Reviewed

Tatsuya Sakai, Ken Haga

PLANT AND CELL PHYSIOLOGY 53 ( 9 ) 1517 - 1534 2012.9

　More details

Language：English

DOI： 10.1093/pcp/pcs111

Web of Science

researchmap
Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant Reviewed

Tamaki Shigeyama, Akiyoshi Tominaga, Susumu Arima, Tatsuya Sakai, Sayaka Inada, Yusuke Jikumaru, Yuji Kamiya, Toshiki Uchiumi, Mikiko Abe, Masatsugu Hashiguchi, Ryo Akashi, Ann M. Hirsch, Akihiro Suzuki

Plant Signaling and Behavior 7 ( 7 ) 1 - 3 2012.7

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.4161/psb.20407

Scopus

PubMed

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
The WAVY GROWTH 3 E3 ligase family controls the gravitropic response in Arabidopsis roots Reviewed

Tatsuya Sakai, Susumu Mochizuki, Ken Haga, Yukiko Uehara, Akane Suzuki, Akiko Harada, Takuji Wada, Sumie Ishiguro, Kiyotaka Okada

PLANT JOURNAL 70 ( 2 ) 303 - 314 2012.4

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1111/j.1365-313X.2011.04870.x

Web of Science

researchmap
Control of auxin transport in the phototropic response(<Feature Article>New development in auxin studies)

Sakai Tatsuya, Haga Ken

Regulation of Plant Growth & Development 47 ( 2 ) 85 - 92 2012

　More details

Language：Japanese Publisher：The Japanese Society for Chemical Regulation of Plants

Phototropism allows plants to change their growth direction in response to the location of the light source. Asymmetric distribution of the phytohormone auxin occurs in response to a phototropic stimulus, and causes differential growth on the two sides of the plant organ and consequent organ bending. Recently, molecular genetic analyses of Arabidopsis have begun to shed light on the molecular mechanisms underlying this response system, including phototropin blue light photoreceptors, phototropin signaling components, auxin transporters and others. This review highlights some of the recent progress on the control of auxin transport in the phototropic response.

DOI： 10.18978/jscrp.47.2_85

CiNii Article

CiNii Books

researchmap
The main auxin biosynthesis pathway in Arabidopsis Reviewed

Kiyoshi Mashiguchi, Keita Tanaka, Tatsuya Sakai, Satoko Sugawara, Hiroshi Kawaide, Masahiro Natsume, Atsushi Hanada, Takashi Yaeno, Ken Shirasu, Hong Yao, Paula McSteen, Yunde Zhao, Ken-ichiro Hayashi, Yuji Kamiya, Hiroyuki Kasahara

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108 ( 45 ) 18512 - 18517 2011.11

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1073/pnas.1108434108

Web of Science

researchmap
Lotus japonicus nodulation is photomorphogenetically controlled by sensing the red/far red (R/FR) ratio through jasmonic acid (JA) signaling Reviewed

Akihiro Suzuki, Lalith Suriyagoda, Tamaki Shigeyama, Akiyoshi Tominaga, Masayo Sasaki, Yoshimi Hiratsuka, Aya Yoshinaga, Susumu Arima, Sakae Agarie, Tatsuya Sakai, Sayaka Inada, Yusuke Jikumaru, Yuji Kamiya, Toshiki Uchiumi, Mikiko Abe, Masatsugu Hashiguchi, Ryo Akashi, Shusei Sato, Takakazu Kaneko, Satoshi Tabata, Ann M. Hirsch

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108 ( 40 ) 16837 - 16842 2011.10

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1073/pnas.1105892108

Web of Science

researchmap
NIMA-related kinases 6, 4, and 5 interact with each other to regulate microtubule organization during epidermal cell expansion in Arabidopsis thaliana Reviewed

Hiroyasu Motose, Takahiro Hamada, Kaori Yoshimoto, Takashi Murata, Mitsuyasu Hasebe, Yuichiro Watanabe, Takashi Hashimoto, Tatsuya Sakai, Taku Takahashi

PLANT JOURNAL 67 ( 6 ) 993 - 1005 2011.9

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1111/j.1365-313X.2011.04652.x

Web of Science

researchmap
Alkoxy-auxins Are Selective Inhibitors of Auxin Transport Mediated by PIN, ABCB, and AUX1 Transporters Reviewed

Etsuko Tsuda, Haibing Yang, Takeshi Nishimura, Yukiko Uehara, Tatsuya Sakai, Masahiko Furutani, Tomokazu Koshiba, Masakazu Hirose, Hiroshi Nozaki, Angus S. Murphy, Ken-ichiro Hayashi

JOURNAL OF BIOLOGICAL CHEMISTRY 286 ( 3 ) 2354 - 2364 2011.1

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1074/jbc.M110.171165

Web of Science

researchmap
Role of the phytochrome and cryptochrome signaling pathways in hypocotyl phototropism Reviewed

Tomoko Tsuchida-Mayama, Tatsuya Sakai, Atsushi Hanada, Yukiko Uehara, Tadao Asami, Shinjiro Yamaguchi

PLANT JOURNAL 62 ( 4 ) 653 - 662 2010.5

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1111/j.1365-313X.2010.04180.x

Web of Science

researchmap
ABCB19/PGP19 stabilises PIN1 in membrane microdomains in Arabidopsis Reviewed

Boosaree Titapiwatanakun, Joshua J. Blakeslee, Anindita Bandyopadhyay, Haibing Yang, Jozef Mravec, Michael Sauer, Yan Cheng, Jiri Adamec, Akitomo Nagashima, Markus Geisler, Tatsuya Sakai, Jiri Friml, Wendy Ann Peer, Angus S. Murphy

PLANT JOURNAL 57 ( 1 ) 27 - 44 2009.1

　More details

Language：English

DOI： 10.1111/j.1365-313X.2008.03668.x

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
The ABC subfamily B auxin transporter AtABCB19 is involved in the inhibitory effects of N-1-Naphthyphthalamic acid on the phototropic and gravitropic responses of Arabidopsis hypocotyls Reviewed

Akitomo Nagashima, Yukiko Uehara, Tatsuya Sakai

PLANT AND CELL PHYSIOLOGY 49 ( 8 ) 1250 - 1255 2008.8

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pcn092

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Mapping of the phosphorylation sites on the phototropic signal transducer, NPH3 Reviewed

Tomoko Tsuchida-Mayama, Michiharu Nakano, Yukiko Uehara, Miho Sano, Noriko Fujisawa, Kiyotaka Okada, Tatsuya Sakai

PLANT SCIENCE 174 ( 6 ) 626 - 633 2008.6

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.plantsci.2008.03.018

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Phytochromes and cryptochromes regulate the differential growth of Arabidopsis hypocotyls in both a PGP19-dependent and a PGP19-independent manner Reviewed

Akitomo Nagashima, Genki Suzuki, Yukiko Uehara, Kensuke Saji, Toshiko Furukawa, Tomokazu Koshiba, Masayo Sekimoto, Shozo Fujioka, Takeshi Kuroha, Mikiko Kojima, Hitoshi Sakakibara, Noriko Fujisawa, Kiyotaka Okada, Tatsuya Sakai

PLANT JOURNAL 53 ( 3 ) 516 - 529 2008.2

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1111/j.1365-313X.2007.03358.x

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Armadillo repeat-containing kinesins and a NIMA-related kinase are required for epidermal-cell morphogenesis in Arabidopsis Reviewed

Tatsuya Sakai, Hannie van der Honing, Miki Nishioka, Yukiko Uehara, Mihoko Takahashi, Noriko Fujisawa, Kensuke Saji, Motoaki Seki, Kazuo Shinozaki, Mark A. Jones, Nicholas Smirnoff, Kiyotaka Okada, Geoffrey O. Wasteneys

PLANT JOURNAL 53 ( 1 ) 157 - 171 2008.1

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1111/j.1365-313X.2007.03327.x

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Distinct leaf developmental and gene expression responses to light quantity depend on blue-photoreceptor or plastid-derived signals, and can occur in the absence of phototropins Reviewed

Enrique Lopez-Juez, John R. Bowyer, Tatsuya Sakai

PLANTA 227 ( 1 ) 113 - 123 2007.12

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1007/s00425-007-0599-7

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Blue light-dependent nuclear positioning in Arabidopsis thaliana leaf cells. Reviewed

Kosei Iwabuchi, Tatsuya Sakai, Shingo Takagi

Plant & cell physiology 48 ( 9 ) 1291 - 8 2007.9

　More details

Language：English

DOI： 10.1093/pcp/pcm095

Web of Science

PubMed

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Interactions among PIN-FORMED and P-glycoprotein auxin transporters in Arabidopsis Reviewed

Joshua J. Blakeslee, Anindita Bandyopadhyay, Ok Ran Lee, Jozef Mravec, Boosaree Titapiwatanakun, Michael Sauer, Srinivas N. Makam, Yan Cheng, Rodolphe Bouchard, Jiri Adamec, Markus Geisler, Akitomo Nagashima, Tatsuya Sakai, Enrico Martinoia, Jiri Friml, Wendy Ann Peer, Angus S. Murphy

PLANT CELL 19 ( 1 ) 131 - 147 2007.1

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1105/tpc.106.040782

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Ca2+ transient induced by extracellular changes in osmotic pressure in Arabidopsis leaves: differential involvement of cell wall-plasma membrane adhesion Reviewed

T Hayashi, A Harada, T Sakai, S Takagi

PLANT CELL AND ENVIRONMENT 29 ( 4 ) 661 - 672 2006.4

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1111/j.1365-3040.2005.01447.x

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
The Arabidopsis WAVY GROWTH 2 protein modulates root bending in response to environmental stimuli Reviewed

S Mochizuki, A Harada, S Inada, K Sugimoto-Shirasu, N Stacey, T Wada, S Ishiguro, K Okada, T Sakai

PLANT CELL 17 ( 2 ) 537 - 547 2005.2

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1105/tpc.104.028530

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
The different growth responses of the Arabidopsis thaliana leaf blade and the petiole during shade avoidance are regulated by Photoreceptors and sugar Reviewed

T Kozuka, G Horiguchi, GT Kim, M Ohgishi, T Sakai, H Tsukaya

PLANT AND CELL PHYSIOLOGY 46 ( 1 ) 213 - 223 2005.1

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/pcp/pci016

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
RPT2 is a signal transducer involved in phototropic response and stomatal opening by association with phototropin 1 in Arabidopsis thaliana Reviewed

S Inada, M Ohgishi, T Mayama, K Okada, T Sakai

PLANT CELL 16 ( 4 ) 887 - 896 2004.4

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1105/tpc.019901

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Functional analysis of each blue light receptor, cry1, cry2, phot1, and phot2, by using combinatorial multiple mutants in Arabidopsis Reviewed

M Ohgishi, K Saji, K Okada, T Sakai

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101 ( 8 ) 2223 - 2228 2004.2

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1073/pnas.0305984101

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
phot1 and phot2 mediate blue light-induced transient increases in cytosolic Ca2+ differently in Arabidopsis leaves Reviewed

A Harada, T Sakai, K Okada

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 100 ( 14 ) 8583 - 8588 2003.7

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1073/pnas.1336802100

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Arabidopsis nph1 and npl1: Blue light receptors that mediate both phototropism and chloroplast relocation Reviewed

T Sakai, T Kagawa, M Kasahara, TE Swartz, JM Christie, WR Briggs, M Wada, K Okada

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 98 ( 12 ) 6969 - 6974 2001.6

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1073/pnas.101137598

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
The phototropin family of photoreceptors Reviewed

WR Briggs, CF Beck, AR Cashmore, JM Christie, J Hughes, JA Jarillo, T Kagawa, H Kanegae, E Liscum, A Nagatani, K Okada, M Salomon, W Rudiger, T Sakai, M Takano, M Wada, JC Watson

PLANT CELL 13 ( 5 ) 993 - 997 2001.5

　More details

Language：English

DOI： 10.1105/tpc.13.5.993

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Arabidopsis NPL1: A phototropin homolog controlling the chloroplast high-light avoidance response Reviewed

T Kagawa, T Sakai, N Suetsugu, K Oikawa, S Ishiguro, T Kato, S Tabata, K Okada, M Wada

SCIENCE 291 ( 5511 ) 2138 - 2141 2001.3

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1126/science.291.5511.2138

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
REPRESSION OFSHOOT GROWTH, a bZIP transcriptional activator, regulates cell elongation by controlling the level of gibberellins Reviewed

J Fukazawa, T Sakai, S Ishida, Yamaguchi, I, Y Kamiya, Y Takahashi

PLANT CELL 12 ( 6 ) 901 - 915 2000.6

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1105/tpc.12.6.901

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
RPT2: A signal transducer of the phototropic response in arabidopsis Reviewed

T Sakai, T Wada, S Ishiguro, K Okada

PLANT CELL 12 ( 2 ) 225 - 236 2000.2

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1105/tpc.12.2.225

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
The identification of DNA binding factors specific for as-1-like sequences in auxin-responsive regions of parA, parB and parC Reviewed

T Sakai, Y Takahashi, T Nagata

PLANT AND CELL PHYSIOLOGY 39 ( 7 ) 731 - 739 1998.7

　More details

Language：English Publishing type：Research paper (scientific journal)

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
Analysis of the promoter of the auxin-inducible gene, parC, of tobacco Reviewed

T Sakai, Y Takahashi, T Nagata

PLANT AND CELL PHYSIOLOGY 37 ( 7 ) 906 - 913 1996.10

　More details

Language：English Publishing type：Research paper (scientific journal)

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247
IDENTIFICATION OF AUXIN-RESPONSIVE ELEMENTS OF PARB AND THEIR EXPRESSION IN APICES OF SHOOT AND ROOT Reviewed

Y TAKAHASHI, T SAKAI, S ISHIDA, T NAGATA

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 92 ( 14 ) 6359 - 6363 1995.7

　More details

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1073/pnas.92.14.6359

Web of Science

researchmap

Other Link： http://orcid.org/0000-0002-1589-6247

▶ display all

MISC

The Role of Dephosphorylation of NPH3 in The Hypocotyl Phototropism of Arabidopsis

木村太郎, 芳賀健, 野村有子, 檜垣匠, 中神弘史, 酒井達也

日本植物生理学会年会(Web) 62nd 2021

　More details

J-GLOBAL

researchmap
S01 Action mechanism of phenylacetic acid as a natural auxin in Arabidopsis

Sugawara Satoko, Mashiguchi Kiyoshi, Tanaka Keita, Hishiyama Shojiro, Sakai Tatsuya, Hanada Kousuke, Kinoshita-Tsujimura Kaori, Yu Hong, Dai Xinhua, Takebayashi Yumiko, Takeda Noriko, Kakimoto Tatsuo, Kawaide Hiroshi, Natsume Masahiro, Estelle Mark, Zhao Yunde, Hayashi Ken-ichiro, Kamiya Yuji, Kasahara Hiroyuki

50 23 - 23 2015.10

　More details

Language：Japanese Publisher：The Japanese Society for Chemical Regulation of Plants

CiNii Article

CiNii Books

researchmap
7. Analysis of auxin function and its action mechanism in Arabidopsis root phototropism

Kimura Taro, Haga Ken, Hayashi Ken-ichiro, Yunde Zhao, Takebayashi Yumiko, Kasahara Hiroyuki, Sakai Tatsuya

48 22 - 22 2013.10

　More details

Language：Japanese Publisher：The Japanese Society for Chemical Regulation of Plants

CiNii Article

CiNii Books

researchmap
4. Structure-activity relationships of IAA biosynthesis inhibitor targeting to YUCCA enzyme

Tsugafune Shinichi, Nishimura Takeshi, Kasahara Hiroyuki, Sakai Tatsuya, Kamiya Yuji, Nozaki Hiroshi, Koshiba Tomokazu, Hayashi Ken-ichiro

47 21 - 21 2012.10

　More details

Language：Japanese Publisher：The Japanese Society for Chemical Regulation of Plants

CiNii Article

CiNii Books

researchmap
2. Analysis of a novel non-polar transport-type auxin in plants

Sugawara Satoko, Mashiguchi Kiyoshi, Tanaka Keita, Hishiyama Shojiro, Sakai Tatsuya, Hanada Kousuke, Kinoshita(Tsujimura) Kaori, Kakimoto Tatsuo, Kawaide Hiroshi, Natsume Masahiro, Zhao Yunde, Hayashi Ken-ichiro, Kamiya Yuji, Kasahara Hiroyuki

47 19 - 19 2012.10

　More details

Language：Japanese Publisher：The Japanese Society for Chemical Regulation of Plants

CiNii Article

CiNii Books

researchmap
TAAとYUCによるインドール‐3‐酢酸の生合成

増口潔, 田中慧太, 酒井達也, 菅原聡子, 夏目雅裕, 川出洋, 花田篤志, 八丈野孝, 白須賢, YAO Hong, MCSTEEN Paula, ZHAO Yunde, 林謙一郎, 神谷勇治, 笠原博幸

日本植物生理学会年会要旨集 53rd 157 2012.3

　More details

Language：Japanese

J-GLOBAL

researchmap
34. Dissection of the main indole-3-acetic acid biosynthesis pathway in Arabidopsis

Mashiguchi Kiyoshi, Tanaka Keita, Sakai Tatsuya, Sugawara Satoko, Natsume Masahiro, Kawaide Hiroshi, Hanada Atsushi, Hayashi Ken-ichiro, Kamiya Yuji, Kasahara Hiroyuki

( 46 ) 50 - 50 2011.10

　More details

Language：Japanese Publisher：The Japanese Society for Chemical Regulation of Plants

The phytohormone auxin plays a fundamental role in various aspects of plant growth and development. Indole-3-acetic acid (IAA) has been known as a major naturally occurring auxin in plants, but its biosynthesis has not been fully understood. Previous studies demonstrated that the YUCCA (YUC) family encodes a plant-specific flavin monooxygenase that catalyzes a rate-limiting step in IAA biosynthesis. More recent studies demonstrated that the Tryptophan Aminotransferase of Arabidopsis 1 (TAA1) converts Trp to indole-3-pyruvic acid (IPA). Both the TAA and YUC families play critical roles throughout plant development, but they are proposed to function in two independent pathways. In this study, we provide evidences that the YUC family acts in the IPA-dependent IAA biosynthesis in Arabidopsis. We found that co-overexpression of TAA and YUC genes dramatically increases IAA production and results in hyper-auxin phenotypes, suggesting a synergistic interaction between TAA and YUC genes in IAA biosynthesis. The LC-ESI-MS/MS analysis of IPA levels in TAA and YUC-related mutants indicates that TAA produces IPA from Trp and YUC converts IPA to IAA. We further showed that recombinant YUC proteins catalyze the direct conversion of IPA to IAA in an NADPH-dependent manner. From these results, we conclude that the IPA pathway is the main IAA biosynthesis pathway in Arabidopsis.

CiNii Article

CiNii Books

researchmap
PHYBシグナリングを介した光の質と量による根粒形成制御機構

重山珠紀, 佐々木雅代, SURIYAGODA Lalith, 富永晃好, 平塚芳美, 内海俊樹, 阿部美紀子, 橋口正嗣, 明石良, 酒井達也, 稲田さやか, 佐藤修正, 金子貴一, 田畑哲之, 東江栄, 有馬進, 鈴木章弘

根の研究 = Root research 20 ( 2 ) 79 - 79 2011.6

　More details

Language：Japanese

CiNii Article

CiNii Books

researchmap
4. Metabolic regulation of IAA in auxin biosynthesis mutants

Tanaka Keita, Sugawara Satoko, Mashiguchi Kiyoshi, Jikumaru Yusuke, Natsume Masahiro, Kawaide Hiroshi, Sakai Tatsuya, Hayashi Ken-ichiro, Kamiya Yuji, Kasahara Hiroyuki

( 45 ) 21 - 21 2010.10

　More details

Language：Japanese Publisher：The Japanese Society for Chemical Regulation of Plants

Auxins have critical roles in plant growth and development. Arabidopsis has multiple pathways for biosynthesis of a naturally occurring auxin, indole-3-acetic acid (IAA): the YUCCA (YUC) pathway, the CYP79B pathway, the indole-3-pyruvic acid (IPA) pathway and the indole-3-acetamide pathway. The YUC and IPA pathways widely exist in plant kingdom. Recently, we found that the CYP79B pathway operates in the crucifer plants producing indole glucosinolates like Arabidopsis, but may not in non-crucifers. It has been demonstrated that cyp79b2 cyp79b3 mutants show IAA-deficient phenotype, but IAA levels are not greatly reduced in these mutants under the normal growth condition. yuc1D mutants show IAA-overproduction phenotype, but IAA levels are slightly increased in yuc1D. To address this question, we analyzed IAA metabolites in Arabidopsis IAA biosynthesis mutants. We found that IAA-amino acid conjugates were significantly decreased in the CYP79B-deficient mutants. In contrast, the levels of IAA-amino acid conjugates were dramatically increased in the YUC-overexpression lines. These results indicate that the IAA-amino acid conjugates may have important roles to maintain IAA levels in IAA biosynthesis-deficient mutants.

CiNii Article

CiNii Books

researchmap
Phot1 controls the de-phosphorylation of NPH3 in response to blue light.

Tomoko Mayama-Tsuchida, Michiharu Nakano, Noriko Fujisawa, Kiyotaka Okada, Tatsuya Sakai

PLANT AND CELL PHYSIOLOGY 48 S232 - S232 2007

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Differential growth of hypocotyls regulated by phytochromes and cryptochromes in Arabidopsis

Akitomo Nmashim, Genki Suzuki, Kensuke Saji, Takeshi Kuroha, Noriko Fujisawa, Yukiko Uchara, Kiyotaka Okada, Tatsuya Sakai

PLANT AND CELL PHYSIOLOGY 48 S199 - S199 2007

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Kinesin-related proteins containing armadillo repeats, antlers1 and antler2, and a NIMA-related kinase, AtNek6, regulate the epidermal cell morphogenesis in Arabidopsis.

Tatsuya Sakai, Miki Nishioka, Yakiko Uehara, Mihoko Takahashi, Noriko Fujisawa, Kensuke Saji, Motoaki Seki, Kazuo Shinozaki, Kiyotaka Okada

PLANT AND CELL PHYSIOLOGY 48 S83 - S83 2007

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Phytochromes and cryptochromes regulate hypocotyl-bending through the action of PGP19 in Arabidopsis

A Nagashima, G Suzuki, K Saji, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 47 S124 - S124 2006

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
phot1 controls the post-transcriptional regulations of RPT2 and NPH3

T Mayama, M Nakano, N Fujisawa, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 47 S160 - S160 2006

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Analysis of the laziness mutant showing a defect in blue-light induced leaf movement

S Inada, M Nakano, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 46 S165 - S165 2005

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Phototropic signal transducer NPH3 is de-modificated by blue light in manner dependent on light intensity

T Mayama, M Nakano, N Fujisawa, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 46 S168 - S168 2005

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Analysis of regulations of root bending in respose to environmental stimuli using wav2 and wav3 mutants

S Mochizuki, A Harada, A Suzuki, S Inada, K Sugimoto-Shirasu, N Stacey, S Ishiguro, T Wada, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 46 S77 - S77 2005

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Functional analysis of each blue light receptor, cry1, cry2, phot1, and phot2, by using combinatorial multiple mutants in Arabidopsis (vol 101, pg 2223, 2004)

M Ohgishi, K Saji, K Okada, T Sakai

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101 ( 15 ) 5696 - 5696 2004.4

　More details

Language：English

DOI： 10.1073/pnas.0401218101

Web of Science

researchmap
Analysis of the stipule-less mutants in Lotus japonicus

N Ishikawa, T Sakai, S Inada, H Tsukaya

PLANT AND CELL PHYSIOLOGY 45 S163 - S163 2004

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Comprehensive preparation of mutants in the model plant of legume, Lotus japonicus.

M Nakano, R Yamamoto, N Fujisawa, S Inada, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 45 S206 - S206 2004

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
RPT2 is a signal transducer involved in stomatal opening mediated by phot1

S Inada, M Ohgishi, T Mayama, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 45 S85 - S85 2004

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Functional analysis of each blue light receptor, cry1, cry2, phot1, phot2 using combinatorial multiple mutant in Arabidopsis

M Ohgishi, K Saji, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 45 S86 - S86 2004

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Function of AtMDR1 for red-light dependent response against pathogen hypocotyl bending

G Suzuki, K Saji, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 45 S1 - S1 2004

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Genetic analysis of green light induced phototropism

T Mayama, T Sakai, K Okada

PLANT AND CELL PHYSIOLOGY 45 S227 - S227 2004

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Analysis of the wav2 mutant exhibiting the strong wavy growth pattern of roots

S Mochizuki, A Harada, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 45 S150 - S150 2004

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Molecular genetic analysis of leaf movement induced by blue light in Lotus japonicus

S Inada, M Takahashi, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 44 S195 - S195 2003

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
phot1 and phot2 mediate blue light-induced transient increase in cytosolic Ca2+ in Arabidopsis leaves

A Harada, T Sakai, K Okada

PLANT AND CELL PHYSIOLOGY 44 S195 - S195 2003

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Analysis of wav2 mutant of Arabidopsis thaliana exhibiting the strong wavy growth pattern of roots

S Mochizuki, A Harada, K Okada, T Sakai

PLANT AND CELL PHYSIOLOGY 44 S37 - S37 2003

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
Analysis of Arabidopsis mutant exhibiting red-light dependent seedling curvature

G Suzuki, T Sakai, K Okada

PLANT AND CELL PHYSIOLOGY 44 S160 - S160 2003

　More details

Language：English Publishing type：Research paper, summary (international conference)

Web of Science

researchmap
ANALYSIS OF ROOT PHOTOTROPISM MUTANT, rpt2, OF ARABIDOPSIS.

SAKAI Tatsuya, WADA Takuji, ISHIGURO Sumie, OKADA Kiyotaka

40 s87 - s87 1999.3

　More details

Language：English

CiNii Article

CiNii Books

researchmap

▶ display all

Research Projects

植物の光源方向認識メカニズムの解明

Grant number：24K02040

2024.4 - 2028.3

System name：科学研究費助成事業

Research category：基盤研究(B)

Awarding organization：日本学術振興会

酒井達也, 芳賀健

　 More details

Grant amount：\18590000 （ Direct Cost: \14300000 、 Indirect Cost：\4290000 ）

researchmap
光屈性におけるシグナル伝達因子レベルの光順応機構の解明

Grant number：21K06211

2021.4 - 2024.3

System name：科学研究費助成事業

Research category：基盤研究(C)

Awarding organization：日本学術振興会

酒井達也

　 More details

Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

植物の芽生えは地上部を光源方向に向かって成長させる光屈性を示す。シロイヌナズナ黄化芽生え胚軸の場合、ヒトの視覚と同程度の光感受性を示し、９桁に及ぶ光強度の異る環境下であっても同じように光屈性応答が誘導される。このような光環境応答においては、光が弱いときには光感受性を高め、逆に光が強いときには光感受性を下げる、高度な光順応機構が必要と考えられる。我々は、光屈性に必須の働きを示すシグナル伝達因子 NPH3 のリン酸化修飾調節が、シグナル伝達因子レベルの光順応機構を生み出すことを発見した。2021年度、本研究成果をまとめ、Plant Physiology誌及びPlant Signaling Behavior 誌で発表することができた。
実験計画に沿い、NPH3 脂質修飾変異体発現体、NPH3天然変性ドメイン欠失変異体を作出し、その表現型を観察中である。またYFP融合変異体も同時に作成し、表現型を観察中である。少なくとも NPH3 天然変性ドメインのほとんどが NPH3 機能に必須ではないことが示唆される結果を得た。NPH3リン酸化調節に働く新奇因子同定のための phot1 エンハンサー変異体選抜は、候補株は複数単離したものの、リン酸化修飾異常の変異体は単離できなかった。phot1自己リン酸化がNPH3細胞内局在調節に与える影響を調べるため、PHOT1自己リン酸化修飾ができない変異体を作成した。現在その表現型を観察中である。

researchmap
Molecular genetic analysis on the asymmetric auxin distribution-independent phototropism

Grant number：18K19329

2018.6 - 2021.3

System name：Grants-in-Aid for Scientific Research

Research category：Grant-in-Aid for Challenging Research (Exploratory)

Awarding organization：Japan Society for the Promotion of Science

Sakai Tatsuya

　 More details

Grant amount：\6370000 （ Direct Cost: \4900000 、 Indirect Cost：\1470000 ）

Etiolated hypocotyls of Arabidopsis seedlings show the phototropic responses in the asymmetric auxin distribution-dependent and -independent manners. We have identified the PP2C gene subfamily, which is probably involved in the asymmetric auxin distribution-independent phototropism. One of them is regulated by light irradiation transcriptionally, and another is phosphorylated in a phototropin-dependent manner. Our phosphoproteome analysis suggests that they function in the phosphorylation modification of many proteins in the etiolated seedlings.

researchmap
Analysis of regulatory mechanisms of phototropism through protein phosphorylations

Grant number：17H03694

2017.4 - 2020.3

System name：Grants-in-Aid for Scientific Research

Research category：Grant-in-Aid for Scientific Research (B)

Awarding organization：Japan Society for the Promotion of Science

Sakai Tatsuya

　 More details

Grant amount：\17940000 （ Direct Cost: \13800000 、 Indirect Cost：\4140000 ）

Plant life is strongly dependent on light and Arabidopsis thaliana phototropin1 (phot1) is a blue-light photoreceptor, i.e. a blue-light-activated Ser/Thr-protein kinase that mediates various light responses including phototropism. Our research goal is an understanding of the phototropin signaling system. In this research, we were focusing on the protein phosphorylation of signaling factors in the phot1 signaling. Our study has revealed that RPT2 suppresses the phot1 autophosphorylation activity and that the phosphorylation status of NPH3 is involved in the photoadaptation mechanism of phototropism as an effector adaptation mechanism.

researchmap
オーキシン不均等分布に依存しない偏差成長誘導機構の解析

Grant number：16H01231

2016.4 - 2018.3

System name：科学研究費助成事業

Research category：新学術領域研究(研究領域提案型)

Awarding organization：日本学術振興会

酒井達也

　 More details

Grant amount：\10400000 （ Direct Cost: \8000000 、 Indirect Cost：\2400000 ）

我々はシロイヌナズナの根の光屈性がオーキシン不均等勾配非依存的に誘導されることを明らかにしたが、一方でオーキシン応答転写因子 ARF7 が根の光屈性に働くことが既に報告されていた。そこでオーキシンシグナル伝達因子の根の光屈性への関与についてさらに遺伝学的解析を行った。その結果、ARF7・ARF19転写因子に加え、AXR3/IAA17 転写調節因子及び TIR1 オーキシン受容体が根の光屈性に正に働くことを明らかにした。本研究成果をまとめ、論文発表した（Kimura et al. [2018] PCP, in press）。
pin3 pin4 pin7 多重変異体及び d6pk 多重変異体は連続光照射誘導の胚軸二次正光屈性をほとんど示さないという報告がある。我々は独自に開発したチューブ法を用いて、pin3 pin4 pin7 及び d6pk0123多重変異体の表現型観察を行った。その結果、我々の観察方法においては、どちらも屈曲の抑制が観察されるものの、未だ連続光照射の胚軸二次正光屈性の反応を示すことを明らかにした。すなわち、胚軸においてもオーキシン不均等勾配非依存的な光屈性誘導機構の存在が示唆された。本研究成果をまとめ、論文発表した（Haga et al. [2018] PCP, in press）。
オーキシン不均等勾配非依存的な光屈性誘導機構を明らかにすべく、pin/d6pk の表現型を促進するエンハンサー突然変異体 6 株を選抜し、その原因遺伝子探索を進めた。その結果、3株が同じPP2C 遺伝子の変異を示した。PP2C遺伝子にT-DNAが挿入された突然変異体を収集し、シングル突然変異体における表現型解析を行った。その結果、pp2cシングル突然変異体においては、赤色光前照射の胚軸光屈性促進効果に異常が観察された。

researchmap
Functional analysis of auxin transcriptional regulators in the phototropic responses

Grant number：26440134

2014.4 - 2017.3

System name：Grants-in-Aid for Scientific Research

Research category：Grant-in-Aid for Scientific Research (C)

Awarding organization：Japan Society for the Promotion of Science

Sakai Tatsuya, KIMURA Taro

　 More details

Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）

Function of Auxin transcriptional regulators were analyzed in the root phototropic response, which is induced independently of asymmetric auxin distribution. Auxin trascriptional facotr ARF7, transcriptional regulator IAA19 and auxin receptor TIR1 were involved in the phototropic response of roots. However, our results suggested that their function are not controlled by the phototropin signaling pathways.

researchmap
光屈性を誘導するフォトトロピンシグナル伝達経路の解析

Grant number：25120710

2013.4 - 2015.3

System name：科学研究費助成事業

Research category：新学術領域研究(研究領域提案型)

Awarding organization：日本学術振興会

酒井達也

　 More details

Grant amount：\16380000 （ Direct Cost: \12600000 、 Indirect Cost：\3780000 ）

rpt2 の詳細な表現型解析と NPH3 リン酸化調節、及びNPH3細胞内局在の解析がつながり、一次正光屈性と二次正光屈性を分ける分子機構が明らかになった。RPT2 は phot1 の光感受性を下げる働きをしており、光によって発現が誘導されると、phot1の光感受性を下げて光寛容を与えている。NPH3は暗所でリン酸化されていると phot1 と結合し、細胞膜に局在してシグナルを流すことを可能にしているが、phot1が活性化してNPH3の脱リン酸化が誘導されるとNPH3がphot1及び細胞膜から解離して不活化することが明らかになった。RPT2によるphot1活性化抑制がNPH3脱リン酸化を抑制し、NPH3のリン酸化状態のゆるやかな回復、NPH3-phot1複合体の回復を誘導し、二次光屈性が誘導されることを明らかにした。この発見は、古典植物生理学で謎とされていた一次正光屈性の仕組みや赤色光照射による光屈性促進機構の解明として高く評価される。またphot1の光感受性変換機構の存在を明らかにしたことにより、phot1分子スイッチの調節技術の開発につながる可能性をひめた研究成果となった。

researchmap
Study of molecular mechanisms underlying phototropic responses independent of auxin lateral distribution

Grant number：24657027

2012.4 - 2014.3

System name：Grants-in-Aid for Scientific Research

Research category：Grant-in-Aid for Challenging Exploratory Research

Awarding organization：Japan Society for the Promotion of Science

HAGA Ken, SAKAI Tatsuya

　 More details

Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

Plants are growing to or away from light direction to adapt to their light environments. The responses, so-called phototropism, have been well-studied for a long time. Auxin, one of plant hormones, is known to stimulate plant growth and thought to be involved in phototropism by changing its endogenous distribution in responses to light irradiation. The present study analyzed phototropic responses of Arabidopsis seedling roots, and demonstrated existence of molecular mechanisms underlying phototropic responses independent of auxin asymmetrical lateral distribution. Our investigations strongly suggested that light stimulation directly controls regulatory components involved in auxin-mediated responses, such as auxin receptors and/or auxin responsive transcription factors. The present results contribute to establishment of a new model responsible for phototropic responses.

researchmap
光屈性におけるオーキシン調節機構の解析

Grant number：23120510

2011.4 - 2013.3

System name：科学研究費助成事業

Research category：新学術領域研究(研究領域提案型)

Awarding organization：日本学術振興会

酒井達也

　 More details

Grant amount：\15080000 （ Direct Cost: \11600000 、 Indirect Cost：\3480000 ）

光屈性は、光環境に応答して植物ホルモン・オーキシン不等分布を誘導し、細胞の伸長の差から偏差成長を生じさせる現象と考えられており、オーキシン調節による植物の成長制御機構のモデルの一つとして研究されている。本研究は光屈性におけるオーキシン調節の分子機構を、シロイヌナズナの分子遺伝学的手法を用いて明らかにすることを目的として行った。昨年度、PIN オーキシン輸送体の網羅的な遺伝学的解析を行い、PIN1, PIN3, PIN7 が光屈性に関与すること、またこれらのPINに依存しない経路が存在することを示唆する結果を得た。
本年度、PINの細胞内局在を制御すると考えられている PINOIDタンパク質キナーゼの光屈性における機能を解析し、PINOID ファミリーは phy 赤色光受容体の活性化によって遺伝子発現が抑制され、これが光屈性の促進に働くこと、光屈性を直接誘導するのに働く phot光受容体シグナリングの下流では働いていないことを明らかにした。さらに、AGC1 キナーゼファミリーが光屈性に関与することが明らかになった。また、新たに光屈性に関与する遺伝子を探索するため、完全長cDNA過剰発現株からの光屈性異常突然変異体選抜を進めた。その結果、小胞輸送調節に働く因子、微小管機能制御因子、タンパク質分解制御因子をコードする３つの遺伝子については、その過剰発現が光屈性異常をひきおこすことを明らかにした。
今後、光屈性誘導機構における AGC タンパク質キナーゼの機能解析、及びPINに依存しない光屈性誘導機構の新たな突然変異体選抜を含む詳細な解析を行うことにより、植物の光環境に適応した成長パターンの調節の分子機構の理解に新しい研究領域を創造することが期待された。

researchmap
Analysis of the phototropin signaling pathways controlling the phototropic responses.

Grant number：22570058

2010 - 2012

System name：Grants-in-Aid for Scientific Research

Research category：Grant-in-Aid for Scientific Research (C)

Awarding organization：Japan Society for the Promotion of Science

SAKAI Tatsuya

　 More details

Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

We have studied about the molecular mechanisms controlling the phototropic responses in plants. Our analyses have indicated that the blue-light photoreceptor phototropin stabilizes the signaling transducer RPT2 proteins, which are required for phototropism and degraded under the dark condition. Furthermore, we have revealed that PIN auxin efflux carriers are necessary for pulse-induced but not continuous light-induced phototropism in Arabidopsis.

researchmap
光環境に適応した植物の成長制御機構の解析

Grant number：18770043

2006 - 2007

System name：科学研究費助成事業

Research category：若手研究(B)

Awarding organization：日本学術振興会

酒井達也

　 More details

Grant amount：\3700000 （ Direct Cost: \3700000 ）

本研究は植物の光環境応答に適応した成長制御機構を解明することを目的に、主にシロイヌナズナ芽生えの光屈性に着目し、研究を行った。光屈性を誘導する光受容体フォトトロピンのシグナル伝達機構を解明するために、そのシグナル伝達因子NPH3のphot1による脱リン酸化メカニズムを解析し、NPH3のリン酸化部位を3カ所同定し、それらのリン酸化部位が光屈性に必須でないことを明らかにした。もう一つのシグナル伝達因子RPT2は光屈性反応を修飾する他の光受容体フィトクロム、クリプトクロムによって転写誘導されること、この転写誘導機構が、フィトクロム、クリプトクロムによる光屈性反応修飾機構の一部であることを明らかにした。これらの結果は、フォトトロピンシグナル伝達因子が、光受容体の活性化によって発現やタンパク質修飾が調節され、光屈性反応を誘導することを示した。
昨年度の研究によって、オーキシン輸送阻害剤NPAは光屈性を負に制御するオーキシン輸送体PGP19に依存して光屈性を阻害し、光屈性を誘導するオーキシン輸送体そのものはその活性が阻害されないこと、PGPl9とNPAが共存したときにのみその活性が阻害されることが明らかになっている。光屈性を誘導するオーキシン輸送体はPIN3が知られているが、pgp19との多重変異体解析を行った結果、NPA非感受性の光屈性誘導に関与するオーキシン輸送体はPIN3以外に存在することを明らかにした。この分子を同定すべく、現在pgp19のサプレッサー変異体選抜および、他のオーキシン輸送体との多重変異体解析を行っている。これらの研究成果をさらに発展させることによって、光シグナリングが具体的にどのオーキシン輸送体の活性を調節し、光屈性を誘導して、植物の生長方向を決定しているのか、近い将来明らかにできるものと考えられる。

researchmap

▶ display all

Teaching Experience

生物学実習III

2023
Institution name：新潟大学
生物学実習I

2022
Institution name：新潟大学
理学基礎演習

2022

-

2024
Institution name：新潟大学
生物学特論 V

2022
Institution name：新潟大学
植物生理学実習

2021
Institution name：新潟大学
専門力アクティブ・ラーニング

2021
Institution name：新潟大学
植物分子遺伝学特論II

2021
Institution name：新潟大学
理学スタディ・スキルズ

2021
Institution name：新潟大学
日本事情自然系A

2020
Institution name：新潟大学
課題研究II(生物学)

2018
Institution name：新潟大学
自然科学基礎実験

2018
Institution name：新潟大学
自然科学総論Ⅳ

2018
Institution name：新潟大学
生物学基礎実習a

2017
Institution name：新潟大学
課題研究I(生物学)

2017
Institution name：新潟大学
生物学総合演習

2017
Institution name：新潟大学
理学スタディ・スキルズ

2017

-

2021
Institution name：新潟大学
生物学基礎演習

2017
Institution name：新潟大学
生物学実習

2017
Institution name：新潟大学
植物生理学実習

2016

-

2019
Institution name：新潟大学
生物学-植物A-

2015
Institution name：新潟大学
生命・食料科学博士セミナーⅠ

2015
Institution name：新潟大学
生物学特論Ⅰ

2015
Institution name：新潟大学
生命・食料科学博士特定研究Ⅰ

2015
Institution name：新潟大学
外国語論文解説・討論Ⅰ

2015
Institution name：新潟大学
植物生理学演習

2014
Institution name：新潟大学
発生生物学I

2014
Institution name：新潟大学
生物英語II

2014
Institution name：新潟大学
生物学－植物A－

2014
Institution name：新潟大学
基礎植物学

2014
Institution name：新潟大学
生物学実験 I

2014

-

2018
Institution name：新潟大学
基礎生物科学実習I

2014

-

2017
Institution name：新潟大学
課題研究I(生物学科)

2014

-

2016
Institution name：新潟大学
課題研究II

2014

-

2016
Institution name：新潟大学
生命・食料科学セミナーBⅡ

2014

-

2015
Institution name：新潟大学
植物形態発生学実習

2014

-

2015
Institution name：新潟大学
生命・食料科学特定研究BⅡ

2014

-

2015
Institution name：新潟大学
文献詳読Ⅱ

2014

-

2015
Institution name：新潟大学
研究発表演習(中間発表)

2014
Institution name：新潟大学
研究発表

2014
Institution name：新潟大学
植物分子遺伝学特論Ⅱ

2013

-

2017
Institution name：新潟大学
生命・食料科学セミナーBⅠ

2013

-

2014
Institution name：新潟大学
文献詳読Ⅰ

2013

-

2014
Institution name：新潟大学
生命・食料科学特定研究BⅠ

2013

-

2014
Institution name：新潟大学
植物分子遺伝学特論Ⅰ

2012

-

2022
Institution name：新潟大学

▶ display all