2024/05/26 更新

写真a

サトウ メグミ
サトウ 恵
SATO Megumi
所属
教育研究院 医歯学系 保健学系列 准教授
医学部 保健学科 検査技術科学専攻 准教授
職名
准教授
外部リンク

学位

  • 熱帯医学博士(PhD in Tropical Medicine) ( 2009年5月   マヒドン大学(タイ王国) )

  • 熱帯医学修士(MSc in Tropical Medicine) ( 2007年4月   マヒドン大学(タイ王国) )

  • 保健衛生学士 ( 2004年2月   学位授与機構 )

研究キーワード

  • 寄生虫学

  • 蠕虫学

  • 検査・診断学

  • 熱帯医学

研究分野

  • ライフサイエンス / 寄生虫学

経歴(researchmap)

  • 新潟大学   大学院保健学研究科   研究教授

    2024年4月 - 現在

      詳細を見る

  • 新潟大学   大学院保健学研究科   准教授

    2019年4月 - 現在

      詳細を見る

  • 新潟大学   大学院保健学研究科   助教

    2011年4月 - 現在

      詳細を見る

経歴

  • 新潟大学   医学部 保健学科 検査技術科学専攻   准教授

    2019年4月 - 現在

  • 新潟大学   医学部 保健学科   助教

    2011年4月 - 2019年3月

学歴

  • Mahidol University   Faculty of Tropical Medicine

    - 2009年6月

      詳細を見る

    国名: タイ王国

    researchmap

  • 産業医科大学   医療技術短期大学   衛生技術学科

    - 1995年3月

      詳細を見る

所属学協会

委員歴

  • 日本熱帯医学会   評議員  

    2022年10月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

取得資格

  • 臨床検査技師

 

論文

  • Ultrasensitive malaria detection system for Anopheles mosquito field surveillance using droplet digital PCR. 国際誌

    Tamasa Araki, Akihide Koyama, Hiro Yoshimura, Ayako Arai, Satoru Kawai, Shuto Sekizawa, Yuko Umeki, Yumiko Saito-Nakano, Takashi Imai, Munehiro Okamoto, Megumi Sato, Wipaporn Thabthimthong, Taratorn Kemthong, Hajime Hisaeda, Suchinda Malaivijitnond, Takeshi Annoura

    Parasitology international   101   102891 - 102891   2024年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Malaria remains a significant global public health concern, with a recent increase in the number of zoonotic malaria cases in Southeast Asian countries. However, limited reports on the vector for zoonotic malaria exist owing to difficulties in detecting parasite DNA in Anopheles mosquito vectors. Herein, we demonstrate for the first time that several Anopheles mosquitoes contain simian malaria parasite DNA using droplet digital PCR (ddPCR), a highly sensitive PCR method. An entomological survey was conducted to identify simian malaria vector species at Phra Phothisat Temple (PPT), central Thailand, recognized for a high prevalence of simian malaria in wild cynomolgus macaques. A total of 152 mosquitoes from six anopheline species were collected and first analyzed by a standard 18S rRNA nested-PCR analysis for malaria parasite which yielded negative results in all collected mosquitoes. Later, ddPCR was used and could detect simian malaria parasite DNA, i.e. Plasmodium cynomolgi, in 25 collected mosquitoes. And this is the first report of simian malaria parasite DNA detection in Anopheles sawadwongporni. This finding proves that ddPCR is a powerful tool for detecting simian malarial parasite DNA in Anopheles mosquitoes and can expand our understanding of the zoonotic potential of malaria transmission between monkeys and humans.

    DOI: 10.1016/j.parint.2024.102891

    PubMed

    researchmap

  • Genetic structure and Rickettsia infection rates in Ixodes ovatus and Haemaphysalis flava ticks across different altitudes

    Maria Angenica F. Regilme, Megumi Sato, Tsutomu Tamura, Reiko Arai, Marcello Otake Sato, Sumire Ikeda, Kozo Watanabe

    PLOS ONE   19 ( 3 )   e0298656 - e0298656   2024年3月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    Ixodid ticks, such as Ixodes ovatus and Haemaphysalis flava, are important vectors of tick-borne diseases in Japan, such as Japanese spotted fever caused by Rickettsia japonica. This study describes the Rickettsia infection rates influenced by the population genetic structure of I.ovatus and H. flava along an altitudinal gradient. A total of 346 adult I. ovatus and 243 H. flava were analyzed for the presence of Rickettsia by nested PCR targeting the 17kDA, gltA, rOmpA, and rOmpB genes. The population genetic structure was analyzed utilizing the mitochondrial cytochrome oxidase 1 (cox1) marker. The Rickettsia infection rates were 13.26% in I. ovatus and 6.17% in H. flava. For I. ovatus, the global F<sub>ST</sub> value revealed significant genetic differentiation among the different populations, whereas H. flava showed non-significant genetic differentiation. The cox1 I. ovatus cluster dendrogram showed two cluster groups, while the haplotype network and phylogenetic tree showed three genetic groups. A significant difference was observed in Rickettsia infection rates and mean altitude per group between the two cluster groups and the three genetic groups identified within I. ovatus. No significant differences were found in the mean altitude or Rickettsia infection rates of H. flava. Our results suggest a potential correlation between the low gene flow in I. ovatus populations and the spatially heterogeneous Rickettsia infection rates observed along the altitudinal gradient. This information can be used in understanding the relationship between the tick vector, its pathogen, and environmental factors, such as altitude, and for the control of tick-borne diseases in Japan.

    DOI: 10.1371/journal.pone.0298656

    researchmap

  • Ov-RPA-CRISPR/Cas12a assay for the detection of Opisthorchis viverrini infection in field-collected human feces. 国際誌

    Orawan Phuphisut, Akkarin Poodeepiyasawat, Tippayarat Yoonuan, Dorn Watthanakulpanich, Charin Thawornkuno, Onrapak Reamtong, Megumi Sato, Poom Adisakwattana

    Parasites & vectors   17 ( 1 )   80 - 80   2024年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Opisthorchis viverrini infection is traditionally diagnosed using the Kato-Katz method and formalin ethyl-acetate concentration technique. However, the limited sensitivity and specificity of these techniques have prompted the exploration of various molecular approaches, such as conventional polymerase chain reaction (PCR) and real-time PCR, to detect O. viverrini infection. Recently, a novel technique known as recombinase polymerase amplification (RPA)-clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) (RPA-CRISPR/Cas) assay was developed as a point-of-care tool for the detection of various pathogens, including viruses and bacteria such as severe acute respiratory syndrome coronavirus 2 and Mycobacterium tuberculosis. This technology has demonstrated high sensitivity and specificity. Therefore, we developed and used the RPA-CRISPR/Cas assay to detect O. viverrini infection in field-collected human feces. METHODS: To detect O. viverrini infection in fecal samples, we developed a CRISPR/Cas12a (RNA-guided endonuclease) system combined with RPA (Ov-RPA-CRISPR/Cas12a). Several fecal samples, both helminth-positive and helminth-negative, were used for the development and optimization of amplification conditions, CRISPR/Cas detection conditions, detection limits, and specificity of the RPA-CRISPR/Cas12a assay for detecting O. viverrini infection. The detection results were determined using a real-time PCR system based on fluorescence values. Additionally, as the reporter was labeled with fluorescein, the detection results were visually inspected using an ultraviolet (UV) transilluminator. A receiver operating characteristic curve (ROC) was used to determine the optimal cutoff value for fluorescence detection. The diagnostic performance, including sensitivity and specificity, of the Ov-RPA-CRISPR/Cas12a assay was evaluated on the basis of comparison with standard methods. RESULTS: The Ov-RPA-CRISPR/Cas12a assay exhibited high specificity for detecting O. viverrini DNA. On the basis of the detection limit, the assay could detect O. viverrini DNA at concentrations as low as 10-1 ng using the real-time PCR system. However, in this method, visual inspection under UV light required a minimum concentration of 1 ng. To validate the Ov-RPA-CRISPR/Cas12a assay, 121 field-collected fecal samples were analyzed. Microscopic examination revealed that 29 samples were positive for O. viverrini-like eggs. Of these, 18 were confirmed as true positives on the basis of the Ov-RPA-CRISPR/Cas12a assay and microscopic examination, whereas 11 samples were determined as positive solely via microscopic examination, indicating the possibility of other minute intestinal fluke infections. CONCLUSIONS: The Ov-RPA-CRISPR/Cas12a assay developed in this study can successfully detect O. viverrini infection in field-collected feces. Due to the high specificity of the assay reported in this study, it can be used as an alternative approach to confirm O. viverrini infection, marking an initial step in the development of point-of-care diagnosis.

    DOI: 10.1186/s13071-024-06134-7

    PubMed

    researchmap

  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for differential identification of adult Schistosoma worms. 国際誌

    Jurena Christiane Ebersbach, Marcello Otake Sato, Matheus Pereira de Araújo, Megumi Sato, Sören L Becker, Issa Sy

    Parasites & vectors   16 ( 1 )   20 - 20   2023年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Schistosomiasis is a major neglected tropical disease that affects up to 250 million individuals worldwide. The diagnosis of human schistosomiasis is mainly based on the microscopic detection of the parasite's eggs in the feces (i.e., for Schistosoma mansoni or Schistosoma japonicum) or urine (i.e., for Schistosoma haematobium) samples. However, these techniques have limited sensitivity, and microscopic expertise is waning outside endemic areas. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has become the gold standard diagnostic method for the identification of bacteria and fungi in many microbiological laboratories. Preliminary studies have recently shown promising results for parasite identification using this method. The aims of this study were to develop and validate a species-specific database for adult Schistosoma identification, and to evaluate the effects of different storage solutions (ethanol and RNAlater) on spectra profiles. METHODS: Adult worms (males and females) of S. mansoni and S. japonicum were obtained from experimentally infected mice. Species identification was carried out morphologically and by cytochrome oxidase 1 gene sequencing. Reference protein spectra for the creation of an in-house MALDI-TOF MS database were generated, and the database evaluated using new samples. We employed unsupervised (principal component analysis) and supervised (support vector machine, k-nearest neighbor, Random Forest, and partial least squares discriminant analysis) machine learning algorithms for the identification and differentiation of the Schistosoma species. RESULTS: All the spectra were correctly identified by internal validation. For external validation, 58 new Schistosoma samples were analyzed, of which 100% (58/58) were correctly identified to genus level (log score values ≥ 1.7) and 81% (47/58) were reliably identified to species level (log score values ≥ 2). The spectra profiles showed some differences depending on the storage solution used. All the machine learning algorithms classified the samples correctly. CONCLUSIONS: MALDI-TOF MS can reliably distinguish adult S. mansoni from S. japonicum.

    DOI: 10.1186/s13071-022-05604-0

    PubMed

    researchmap

  • Detection and persistence of environmental DNA (eDNA) of the different developmental stages of a vector mosquito, Culex pipiens pallens 査読

    Masayuki K. Sakata, Megumi Sato, Marcello Otake Sato, Tomoe Watanabe, Honami Mitsuishi, Tomoyuki Hikitsuchi, Jun Kobayashi, Toshifumi Minamoto

    PLOS ONE   17 ( 8 )   e0272653 - e0272653   2022年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    Preventing mosquito-borne infectious diseases requires that vector mosquitoes are monitored and controlled. Targeting immature mosquitoes (eggs, larvae, and pupae), which have less mobility than adults, is an effective management approach. However, conducting these surveys is often difficult due to the limitations of morphological classification and survey costs. The application of environmental DNA (eDNA) analysis can solve these issues because it allows easy estimation of species distribution and morphology-independent species identification. Although a few previous studies have reported mosquito eDNA detection, there is a gap in knowledge regarding the dynamics related to the persistence of immature mosquito eDNA. We used Culex pipiens pallens, a vector of West Nile fever, as a model species. First, we developed a species-specific detection assay and confirmed its specificity using in silico and in vitro tests. Next, we conducted laboratory experiments using breeding tanks. Water samples were collected at each developmental stage. In addition, water samples were collected daily until the seventh day after emergence from the pupae. We quantified eDNA using real-time PCR with the developed assay to investigate the dynamics of mosquito eDNA. The specificity of the developed assay was confirmed by in silico and in vitro tests. Mosquito eDNA was detected at all developmental stages and detected up to seven days after emergence of pupae. In particular, high concentrations of eDNA were detected immediately after hatching from eggs and after emergence from pupae. Highly frequent positive eDNA signals were continuously detected between egg hatching and pupa hatching. Mosquito eDNA was detected immediately after the eggs were introduced, and eDNA-positive detections continued until pupae emergence, suggesting that eDNA analysis is useful for monitoring mosquito larvae. In the future, monitoring immature mosquitoes using eDNA analysis will contribute to prevent mosquito-borne infectious diseases.

    DOI: 10.1371/journal.pone.0272653

    researchmap

  • Circulating microRNAs as Biomarkers of Hepatic Fibrosis in Schistosomiasis Japonica Patients in the Philippines. 国際誌

    Ian Kim B Tabios, Marcello Otake Sato, Ourlad Alzeus Gaddi Tantengco, Raffy Jay C Fornillos, Masashi Kirinoki, Megumi Sato, Raniv D Rojo, Ian Kendrich C Fontanilla, Yuichi Chigusa, Paul Mark B Medina, Mihoko Kikuchi, Lydia R Leonardo

    Diagnostics (Basel, Switzerland)   12 ( 8 )   2022年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Host-derived microRNAs (miRNAs) play important regulatory roles in schistosomiasis-induced hepatic fibrosis. This study analyzed selected serum miRNAs among Filipino schistosomiasis japonica patients with ultrasound (US)-detectable hepatic fibrosis. A prospective cohort study design with convenience sampling was employed from 2017 to 2019. The study sites were eight endemic barangays in Leyte, Philippines. Eligible chronic schistosomiasis patients with varying severities of hepatic fibrosis were enrolled in the cohort and serially examined at 6, 12, and 24 months from baseline. Baseline serum miR-146a-5p, let-7a-5p, miR-150-5p, miR-122-5p, miR-93-5p, and miR200b-3p were measured using RT-qPCR. A total of 136 chronic schistosomiasis patients were included in this prospective cohort study. Approximately, 42.6% had no fibrosis, 22.8% had mild fibrosis, and 34.6% had severe fibrosis at baseline The serum levels of the antifibrotic miR-146a (p &lt; 0.0001), miR-150 (p = 0.0058), and let-7a (p &lt; 0.0001) were significantly lower in patients with hepatic fibrosis while the profibrotic miR-93 (p = 0.0024) was elevated. miR-146a-5p (AUC = 0.90, 95% CI [0.84, 0.96], p &lt; 0.0001) has the most promising potential to differentiate patients with (n = 78) versus without (n = 58) hepatic fibrosis. The baseline level of serum miR-146-5p was significantly different in patients with progressive fibrosis (n = 17) compared to those who never developed fibrosis (n = 30, p &lt; 0.01) or those who had fibrosis reversal (n = 20, p &lt; 0.01) after 24 months. These findings demonstrate the potential utility of serum miRNAs, particularly of miR-146a, as a supplementary tool for assessing hepatic fibrosis in chronic schistosomiasis japonica patients.

    DOI: 10.3390/diagnostics12081902

    PubMed

    researchmap

  • Diagnostic Performance of Parasitological, Immunological, Molecular, and Ultrasonographic Tests in Diagnosing Intestinal Schistosomiasis in Fieldworkers From Endemic Municipalities in the Philippines 査読

    Ian Kim B. Tabios, Marcello Otake Sato, Ourlad Alzeus G. Tantengco, Raffy Jay C. Fornillos, Masashi Kirinoki, Megumi Sato, Raniv D. Rojo, Ian Kendrich C. Fontanilla, Yuichi Chigusa, Paul Mark B. Medina, Mihoko Kikuchi, Lydia R. Leonardo

    Frontiers in Immunology   13   2022年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Media SA  

    Schistosomiasis remains to ha/ve a significant public health impact in the Philippines. The Kato-Katz (K-K) technique is the reference standard and most used technique for definitive diagnosis of intestinal schistosomiasis for control programs in endemic regions. However, this has a very low sensitivity when applied in areas of low endemicity and patients with light infection. Hence, this study determined the diagnostic performance of immunological, molecular, parasitological, and ultrasonographic tests in diagnosing intestinal schistosomiasis in endemic municipalities in the Philippines. We performed a community-based cross-sectional study to determine the positivity of schistosomiasis in Leyte, Philippines. The diagnostic performance of five different detection techniques: (1) three stool K-K with duplicate smears; (2) soluble egg antigen IgG ELISA; (3) urine point-of-care circulating cathodic antigen (POC-CCA) test; (4) detection of Schistosoma japonicum circulating DNA (SjcDNA) in serum and urine samples; (5) focused abdominal ultrasound (US), were also obtained in this study. Multiple stool examinations enhanced the sensitivity of K-K from 26.2% (95% CI [16.4, 38.8]) with single stool to 53.8% (95% CI [41.1, 66.1]) and 69.2% (95% CI [56.4, 80.0]) with two and three stools from consecutive days, respectively. Among the SjcDNA nucleic acid amplification test (NAAT)-based detection assays, loop-mediated isothermal amplification (LAMP) PCR using sera had the highest sensitivity at 92.3% (95% CI [82.2, 97.1]) with LAMP consistently identifying more positive cases in both serum and urine samples. This study showed that single stool K-K, which remains the only diagnostic test available in most endemic areas in the Philippines, had low sensitivity and failed to identify most patients with light infection. SjcDNA detection assay and POC-CCA urine test were more sensitive than stool microscopy in detecting schistosomiasis. On the other hand, US was less sensitive than the widely utilized K-K technique in diagnosing schistosomiasis. This study emphasizes the need to revisit the use of single stool K-K in the surveillance and case detection of schistosomiasis in endemic areas of the Philippines. The availability of advanced and more sensitive diagnostic tests will help better control, prevent, and eliminate schistosomiasis in the country.

    DOI: 10.3389/fimmu.2022.899311

    researchmap

  • Knowledge, attitudes, and practices regarding tick-borne diseases among an at-risk population living in Niigata prefecture, Japan. 査読 国際誌

    Taichi Narita, Hansani Madushika Abeywickrama, Marcello Otake Sato, Kozo Watanabe, Reiko Arai, Tsutomu Tamura, Megumi Sato

    Plos One   17 ( 6 )   e0270411   2022年6月

     詳細を見る

    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: This study examined the knowledge, attitudes, and practices (KAP) of an at-risk population living in Niigata prefecture regarding tick-borne diseases (TBDs) and preventive strategies. METHODS: A cross-sectional questionnaire-based study was conducted to assess the KAP of the community. RESULTS: In total, 186 responses were received. Among the respondents, 130 (69·9%) were men, and the mean age was 51.1 (14·3). Nine (4·8%) respondents reported having experienced tick bites. Of the respondents, 44 (23.7%) knew about both scrub typhus and severe fever with thrombocytopenia syndrome, while 156 (83·9%) and 71 (38·2%) recognized limiting skin exposure and use of insect repellents as preventive measures, respectively. The attitudes towards TBDs: being worried about tick bites (p = 0·018) and interested in preventing TBDs (p = 0·001), were significantly higher among women than men. About 75% of the respondents reported taking preventive measures against tick bites, and limiting skin exposure was the most frequently applied method (69·9%). Insect repellents were used by 58 (31·2%) respondents. Age (p = 0·049), being worried about tick bites (p = 0·046), and knowledge of ticks score (p = 0·024) were the significant independent predictors of practicing countermeasures. CONCLUSION: We identified gaps in knowledge and practices regarding TBDs. Public health interventions should be implemented to improve public awareness of TBDs.

    DOI: 10.1371/journal.pone.0270411

    PubMed

    researchmap

  • Distribution and prevalence of Taenia hydatigena, Taenia multiceps, and Mesocestoides spp. in Mongolian sheepdogs 査読 国際誌

    Janjiv Temuujin, Gelegbalsan Ulziijargal, Chultemsuren Yeruult, Zagd Amarbayasgalan, Tangad Mungunzaya, Uudus Bayarsaikhan, Janjiv Khulan, Toni Wandra, Marcello Otake Sato, Choijilsuren Gantstetseg, Baldorj Ochirkhuyag, Megumi Sato, Hiroshi Yamasaki, Myadagsuren Narankhajid

    Veterinary Parasitology: Regional Studies and Reports   28   100680 - 100680   2022年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Gastrointestinal parasite infections in livestock and companion animals in Mongolia have not been investigated sufficiently. This study aimed to determine the prevalence of cyclophyllid cestodes (Taeniidae and Mesocestoididae) in Mongolian sheepdogs using copro-DNA analysis. Sheepdog fecal samples (n = 1242) were collected from five ecological zones (mountain taiga, forest-steppe, steppe, desert-steppe, and desert) and four geographical regions (Western, Khangai, Central, and Eastern) within 20 of the country's 21 provinces. Among the 1242 samples, 201 (16.2%) tested positive for mitochondrial cytochrome c oxidase subunit 1 (cox1) and ribosomal 12S rRNA encoding genes of cyclophyllid cestodes. Prevalence in the mountain taiga, forest-steppe, steppe, desert-steppe, and desert zones was 29.2%, 15.4%, 15.1%, 20.1%, and 9.1%, respectively. Prevalence in the Western, Khangai, Central, and Eastern regions was 19.9%, 18.6%, 12.1%, and 12.8%, respectively. Taenia hydatigena, Taenia multiceps, and two Mesocestoides species (Mesocestoides sp.1 and Mesocestoides sp.2) were identified. T. hydatigena was found in the samples from all 20 provinces (all five zones and four regions), while T. multiceps was detected in the samples from 19 provinces (all five zones and four regions). Mesocestoides sp.1 infection was detected in the samples from all zones (except desert) and regions; it was detected in 14 provinces. Mesocestoides sp.2 infection was detected in the samples from all zones (except mountain taiga) and regions and found in seven provinces. Cyclophyllidea infection in sheepdogs is highly prevalent across Mongolia, representing a zoonotic risk. Implementation of a surveillance program for sheepdogs and their owners, and the wild animals in all ecological zones should be considered. In addition, control measures, including public awareness campaigns, especially for sheepdog owners, and periodic deworming of sheepdogs are warranted.

    DOI: 10.1016/j.vprsr.2021.100680

    PubMed

    researchmap

  • Comparative population genetic structure of two ixodid tick species (Acari:Ixodidae) (Ixodes ovatus and Haemaphysalis flava) in Niigata prefecture, Japan 査読

    Maria Angenica F. Regilme, Megumi Sato, Tsutomu Tamura, Reiko Arai, Marcello Otake Sato, Sumire Ikeda, Maribet Gamboa, Michael T. Monaghan, Kozo Watanabe

    Infection, Genetics and Evolution   104999 - 104999   2021年7月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.meegid.2021.104999

    researchmap

  • Diversity and distribution of ticks in Niigata prefecture, Japan (2016-2018): Changes since 1950. 査読 国際誌

    Megumi Sato, Sumire Ikeda, Reiko Arai, Miwako Kato, Junko Aoki, Akiko Nishida, Kaori Watanabe, Chika Hirokawa, Kozo Watanabe, Maria Angenica F Regilme, Mami Sato, Marcello Otake Sato, Tsutomu Tamura

    Ticks and tick-borne diseases   12 ( 3 )   101683 - 101683   2021年2月

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We performed tick surveys in all regions (Kaetsu, Chuetsu, Joetsu, and Sado) of the Niigata prefecture, Japan. A total of 105 field surveys were done from 2016 to 2018 in 41 sites, from north to south, in the prefecture. All 4806 ticks collected were identified and classified by species, sex, and developmental stage. Twelve species were recorded: Dermacentor taiwanensis, Haemaphysalis flava, Haemaphysalis hystricis, Haemaphysalis japonica, Haemaphysalis longicornis, Haemaphysalis megaspinosa, Ixodes ovatus, Ixodes nipponensis, Ixodes persulcatus, Ixodes monospinosus, Ixodes columnae, and Ixodes turdus. The major tick species in Niigata prefecture were H. flava, H. longicornis, and I. ovatus and they comprised 93.4% of all samples. These three species have one generation per year. Climatic and anthropogenic factors may be involved in the substantial change of the endemic species composition from a previous tick survey (1959) in the Niigata prefecture. These factors include increasing temperatures, introduction of new hosts such as the wild boar, highway construction, and a rural exodus facilitating animal migration and reproduction. Tick hosts suitable for the transmission of Japanese spotted fever, Lyme borreliosis, and SFTS occur in Niigata prefecture. Heightened awareness of these three tick-borne diseases is needed for preparation and disease prevention.

    DOI: 10.1016/j.ttbdis.2021.101683

    PubMed

    researchmap

  • Spotted fever group rickettsiae (SFGR) detection in ticks following reported human case of Japanese spotted fever in Niigata Prefecture, Japan. 査読 国際誌

    Reiko Arai, Megumi Sato, Miwako Kato, Junko Aoki, Akiko Nishida, Kaori Watanabe, Chika Hirokawa, Sumire Ikeda, Kozo Watanabe, Maria Angenica F Regilme, Marcello Otake Sato, Tsutomu Tamura

    Scientific reports   11 ( 1 )   2595 - 2595   2021年1月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Japanese spotted fever, a tick-borne disease caused by Rickettsia japonica, was firstly described in southwestern Japan. There was a suspicion of Rickettsia japonica infected ticks reaching the non-endemic Niigata Prefecture after a confirmed case of Japanese spotted fever in July 2014. Therefore, from 2015 to 2017, 38 sites were surveyed and rickettsial pathogens were investigated in ticks from north to south of Niigata Prefecture including Sado island. A total of 3336 ticks were collected and identified revealing ticks of three genera and ten species: Dermacentor taiwanensis, Haemaphysalis flava, Haemaphysalis hystricis, Haemaphysalis longicornis, Haemaphysalis megaspinosa, Ixodes columnae, Ixodes monospinosus, Ixodes nipponensis, Ixodes ovatus, and Ixodes persulcatus. Investigation of rickettsial DNA showed no ticks infected by R. japonica. However, three species of spotted fever group rickettsiae (SFGR) were found in ticks, R. asiatica, R. helvetica, and R. monacensis, confirming Niigata Prefecture as a new endemic area to SFGR. These results highlight the need for public awareness of the occurrence of this tick-borne disease, which necessitates the establishment of public health initiatives to mitigate its spread.

    DOI: 10.1038/s41598-021-81587-9

    PubMed

    researchmap

  • Human toxocariasis, a silent helminthic disease revealed in Savannakhet, Lao PDR. 査読 国際誌

    Megumi Sato, Marcello Otake Sato, Jitra Waikagul, Tiengkham Pongvongsa, Surapol Sanguankiat, Tipparayat Yoonuan, Sengchanh Kounnavong, Satoru Kawai, Hiroshi Yamasaki, Kazuhiko Moji

    One health (Amsterdam, Netherlands)   11   100191 - 100191   2020年12月

     詳細を見る

    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    Toxocariasis is a zoonotic helminthiasis caused by the migrating larvae of Toxocara canis and T. cati, common roundworms of dogs and cats. Our previous study in Savannakhet Province of Lao PDR showed an infection rate of 44.1% of Toxocara spp. in dogs. Thus, we investigate if this previous high prevalence in the definitive hosts influenced the occurrence of human toxocariasis. For that we used a 38 kDa recombinant protein derived from T. canis larvae excretion secretion products in ELISA. Human serum samples were collected in the Lahanam area of Savannakhet province. The population attending the study in Lahanam village were aged from 7 to 59 years old (y/o) 65.9% (54/82) were male and 34.1% (28/82) were female. The total percentage of seropositivity to Toxocara sp. was 30.4% (25/82). Males were more likely to test positive for toxocariasis with a risk ratio of 2.70 (CI95 0.87-4.93). No significant differences between ages were seen. However, it was possible to observe an increase of optical density (OD) values in ELISA according to age. The awareness of the health system on the high prevalence of seropositivity to Toxocara sp. in Savannakhet can prevent irreversible consequences as permanent vision loss and seizures caused by this silent chronic disease revealed in the Lahanam area.

    DOI: 10.1016/j.onehlt.2020.100191

    PubMed

    researchmap

  • Preliminary Report on the Effect of Savanna Plants Leucaena leucocephala, Parkia platycephala and Senna alata against Eggs and Immature Stages of Trichostrongylid Nematodes In Vitro. 査読 国際誌

    Benta Natânia Silva Figueiredo, Marcello Otake Sato, Laiane Teixeira Sousa Moura, Sandra Maria Botelho Mariano, Tarso da Costa Alvim, Ilsamar Mendes Soares, Satoru Kawai, Sergio Donizeti Ascêncio, Helcileia Dias Santos, Joseilson Alves Paiva, Megumi Sato, Viviane Mayumi Maruo

    Pathogens (Basel, Switzerland)   9 ( 12 )   2020年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The current study evaluated the anthelmintic effect of different extracts of Leucaena leucocephala, Parkia platycephala, and Senna alata on trichostrongylid eggs and infective larvae and determined the potential active components of each plant. Dried and macerated plant material was concentrated using rotaevaporation to obtain the crude extract (CE), followed by solvent partitioning to obtain hexanic (HexE), acetatic (AcE), and butanolic (BuE) extracts used for phytochemical analysis and anthelmintic efficacy testing in vitro. All the crude and partitioned extracts tested showed inhibition activity in the hatching of trichostrongylid eggs. Larvicidal efficacy was observed at CE concentrations of 2.5, 5.0, and 7.5 mg/mL for P. platycephala and S. alata. However, L. leucocephala CE did not significantly reduce the number of living larvae in the tested concentrations. Chromatographic analysis revealed several active metabolites; gallic acid, ellagic acid, naringin, morin, and kaempferol on AcE of P. platycephala; gallic acid, rutin, and ellagic acid on BuE of P. platycephala; and gallic acid and naringin on BuE of L. leucocephala. The extracts of P. platycephala, L. leucocephala, and S. alata leaves showed egg hatching inhibition and larvicidal activity, probably produced by tannins and flavonoids, which may act alone or by synergism.

    DOI: 10.3390/pathogens9120986

    PubMed

    researchmap

  • Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi. 査読 国際誌

    Fritz Ivy C Calata, Camille Z Caranguian, Jillian Ela M Mendoza, Raffy Jay C Fornillos, Ian Kim B Tabios, Ian Kendrich C Fontanilla, Lydia R Leonardo, Louie S Sunico, Satoru Kawai, Yuichi Chigusa, Mihoko Kikuchi, Megumi Sato, Toshifumi Minamoto, Zenaida G Baoanan, Marcello Otake Sato

    Pathogens (Basel, Switzerland)   8 ( 4 )   160   2019年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the environment as evidenced by snails infected with schistosome larval stages, an emerging technology known as environmental DNA (eDNA) detection provides an alternative method. Previous reports showed that O. hupensis quadrasi eDNA could be detected in water, but no reports have been made on its detection in soil. METHODS: This study, thus focused on the detection of O. hupensis quadrasi eDNA from soil samples collected from two selected schistosomiasis-endemic barangays in Gonzaga, Cagayan Valley using conventional and TaqMan-quantitative (qPCR) PCRs. RESULTS: The results show that qPCR could better detect O. hupensis quadrasi eDNA in soil than the conventional method. In determining the possible distribution range of the snail, basic edaphic factors were measured and correlated with the presence of eDNA. The eDNA detection probability increases as the pH, phosphorous, zinc, copper, and potassium content increases, possibly indicating the conditions in the environment that favor the presence of the snails. A map was generated to show the probable extent of the distribution of the snails away from the body of the freshwater. CONCLUSION: The information generated from this study could be used to determine snail habitats that could be possible hotspots of transmission and should, therefore, be targeted for snail control or be fenced off from human and animal contact or from the contamination of feces by being a dumping site for domestic wastes.

    DOI: 10.3390/pathogens8040160

    PubMed

    researchmap

  • Ascaris lumbricoides found in ashore corpses from Korean peninsula to Japan. 国際誌

    Megumi Sato, Kazuhisa Funayama, Reiji Hoshi, Hisakazu Takatsuka, Marcello Otake Sato

    Parasitology international   70   1 - 4   2019年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Yearly, several reports of unknown boats and corpses brought by the Tsushima Current are found ashore Japanese coast. Niigata prefecture had the highest number of the drifting ashore corpses in Japan with 45.7% (16/35) in 2017. Corpses from North Korea, confirmed by documents and photos were autopsied and in 3/16 was possible to recover worms full of eggs, morphologically identified as ascarids. Further molecular analysis of ITS1, 5.8S rDNA and ITS2 sequences confirmed all specimens were Ascaris lumbricoides. The contamination level by Ascaris lumbricoides eggs in the coast, the health impact and consequences of the epidemiological bridging produced by this forced migration in public health should be investigated. Moreover, control of helminthiases might be a necessary task in North Korea.

    DOI: 10.1016/j.parint.2019.01.002

    PubMed

    researchmap

  • Occurrence of Bovine Cysticercosis in Two Regions of the State of Tocantins-Brazil and the Importance of Pathogen Identification. 査読 国際誌

    Benta Natânia Silva Figueiredo, Ricardo Alencar LIBóRIO, Megumi Sato, Camila Figueira da Silva, Ronaldo Alves Pereira-Junior, Yuichi Chigusa, Satoru Kawai, Marcello Otake Sato

    Pathogens (Basel, Switzerland)   8 ( 2 )   66   2019年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI} {AG  

    Bovine cysticercosis, caused by Taenia saginata metacestodes, is the cause of significant economic losses to the meat production chain by condemnation and downgrading of infected carcasses. It is also a public health issue causing human taeniasis. This study evaluated the occurrence of bovine cysticercosis at the meat inspection procedures in slaughterhouses of south and north regions of the Tocantins State in Brazil. Specimens identified as cysts of T. saginata were collected and analyzed by molecular (PCR) and histopathological techniques. The cysts were collected from March to December of 2010 in slaughterhouses located in the cities of Alvorada (South) and Araguaína (North). The frequency of cystic lesions during the study was 0.033% (53/164,091) with 69.81% of calcified lesions and 30.9% of live cysts at meat inspection. From 14 samples submitted to molecular analysis, 28.57% (4/14) were positive for T. saginata. The histopathological analysis of the non-T. saginata samples showed lesions suggestive of granuloma and hydatid disease. The results indicated that the identification of the etiological agent is difficult by macroscopic inspection, emphasizing the need to associate specific diagnostic methods at meat inspection in abattoirs. In addition, species-specific PCR would be an effective tool for diagnosis, monitoring, and identifying cysticercosis, assisting the conventional tests.

    DOI: 10.3390/pathogens8020066

    PubMed

    researchmap

  • Environmental DNA metabarcoding to detect pathogenic Leptospira and associated organisms in leptospirosis-endemic areas of Japan. 査読 国際誌

    Yukuto Sato, Masaru Mizuyama, Megumi Sato, Toshifumi Minamoto, Ryosuke Kimura, Claudia Toma

    Scientific reports   9 ( 1 )   6575 - 6575   2019年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Leptospires, which cause the zoonotic disease leptospirosis, persist in soil and aqueous environments. Several factors, including rainfall, the presence of reservoir animals, and various abiotic and biotic components interact to influence leptospiral survival, persistence, and pathogenicity in the environment. However, how these factors modulate the risk of infection is poorly understood. Here we developed an approach using environmental DNA (eDNA) metabarcoding for detecting the microbiome, vertebrates, and pathogenic Leptospira in aquatic samples. Specifically, we combined 4 sets of primers to generate PCR products for high-throughput sequencing of multiple amplicons through next-generation sequencing. Using our method to analyze the eDNA of leptospirosis-endemic areas in northern Okinawa, Japan, we found that the microbiota in each river shifted over time. Operating taxonomic units corresponding to pathogenic L. alstonii, L. kmetyi, and L. interrogans were detected in association with 12 nonpathogenic bacterial species. In addition, the frequencies of 11 of these species correlated with the amount of rainfall. Furthermore, 10 vertebrate species, including Sus scrofa, Pteropus dasymallus, and Cynops ensicauda, showed high correlation with leptospiral eDNA detection. Our eDNA metabarcoding method is a powerful tool for understanding the environmental phase of Leptospira and predicting human infection risk.

    DOI: 10.1038/s41598-019-42978-1

    PubMed

    researchmap

  • Odds, challenges and new approaches in the control of helminthiasis, an Asian study. 国際誌

    Marcello Otake Sato, Poom Adsakwattana, Ian Kendrich C Fontanilla, Jun Kobayashi, Megumi Sato, Tiengkhan Pongvongsa, Raffy Jay C Fornillos, Jitra Waikagul

    Parasite epidemiology and control   4   e00083   2019年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The time is passing, and the worms are still a major struggle for local people in Asian countries, especially the less empowered and in a situation of social vulnerability. We are working in the field in Laos, Thailand, and the Philippines where the usual control programs based only on human treatment are partially effective. Areas with mass drug administration could diminish, but not eliminate STHs of endemic areas. The persistence of helminthic NTDs in the environment and animal hosts makes the eradication a very difficult task. Great changes in the landscapes of endemic areas, such as construction of dams, can change the fauna and the lifestyle of local people. Those changes can improve infrastructure, but it can also lead to social vulnerability. The challenge, then, is to conceive new and directed control programs for helminthiasis based on multi- and transdisciplinary approaches diminishing the health gap in a globalized world. In this short review, we summarize the actual scenario concerning the main helminths in Southeast Asia and how an environmental DNA approach and the use of GIS could contribute to surveillance and control programs.

    DOI: 10.1016/j.parepi.2018.e00083

    PubMed

    researchmap

  • Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines. 査読 国際誌

    Raffy Jay C Fornillos, Marcello Otake Sato, Ian Kim B Tabios, Megumi Sato, Lydia R Leonardo, Yuichi Chigusa, Toshifumi Minamoto, Mihoko Kikuchi, Emelda R Legaspi, Ian Kendrich C Fontanilla

    PloS one   14 ( 11 )   e0224617   2019年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In recent years, the prevalence and infection intensity of Schistosoma japonicum in endemic areas of the Philippines have significantly decreased due to yearly population-based treatment strategies, yet transmission rates remain high and uninterrupted. An important indicator of active disease transmission is the presence of Schistosoma japonicum and its snail intermediate host Oncomelania hupensis quadrasi in freshwater habitats. In this study, we sought to apply a species-specific real-time PCR (qPCR) assay for the detection of S. japonicum and O. hupensis quadrasi in freshwater samples using environmental DNA approach that can complement the commonly utilized malacological survey in determining potential transmission foci in order to have a more effective snail surveillance strategy for schistosomiasis japonica in endemic areas. The newly developed assay was specific to S. japonicum and O. hupensis quadrasi with no amplification detected against non-target trematode Fasciola spp. and snails such as Lymnaea spp., Pomacea canaliculata, and Melanoides spp. that typically co-exist in the same environment. The assay effectiveness was determined using 19 environmental water samples collected from Northern Samar (N = 5 sites), Leyte (N = 11 sites) and Compostela Valley (N = 3 sites) and compared to malacological survey for determining O. hupensis quadrasi snail colonies and snail crushing to visualize S. japonicum cercariae. TaqMan qPCR targeting a short fragment of the cytochrome c oxidase subunit 1 (cox1) gene was positive for S. japonicum in 9 sites, for O. hupensis quadrasi in 9 sites, and for both S. japonicum and O. hupensis quadrasi in 5 sampling sites. Moreover, it was able to detect O. hupensis quadrasi in 3 out of 12 sites found negative and 6 out of 7 sites found positive through malacological survey, and in 4 of the 5 snail sites positive for snails with cercariae. Overall, this method can complement malacological surveys for monitoring of schistosomes in endemic areas of the Philippines, especially those with high risk of human infection.

    DOI: 10.1371/journal.pone.0224617

    PubMed

    researchmap

  • Usefulness of environmental DNA for detecting Schistosoma mansoni occurrence sites in Madagascar. 査読 国際誌

    Marcello Otake Sato, Armand Rafalimanantsoa, Charles Ramarokoto, Alain Marcel Rahetilahy, Pascaline Ravoniarimbinina, Satoru Kawai, Toshifumi Minamoto, Megumi Sato, Masashi Kirinoki, Voahangy Rasolofo, Mathilde De Calan, Yuichi Chigusa

    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases   76   130 - 136   2018年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    OBJECTIVES: Schistosomiasis is an important disease in Madagascar, and several studies on the disease have focused on the occurrence of the parasite in humans. However, the range of the pathogen in the environment and its impact on human infection is difficult to predict. An environmental DNA (eDNA) detection system for Schistosoma mansoni was developed to improve schistosomiasis eco-epidemiology studies. METHODS: Primers and probes were designed and tested in experimental biotopes. The field study was conducted in Maevatanana District of Madagascar. Seven water sources with human use were sampled, with a total of 21 water samples collected. Snails were collected, and patients were examined by ultrasound to determine the occurrence of schistosomiasis in the study area. RESULTS: One water source with active transmission was identified through the detection of S. mansoni eDNA in the water and the intermediate host Biomphalaria pfeifferi collected from the same water source. People with clinical schistosomiasis were found in the area, reinforcing the findings. CONCLUSIONS: The application of eDNA in eco-epidemiology enables the determination of hot spots and safe spots in endemic areas, constituting an alternative ecological tool for follow-up and monitoring of control programs for schistosomiasis, and contributing information on water safety for improving the standard of living of the people in endemic areas.

    DOI: 10.1016/j.ijid.2018.08.018

    PubMed

    researchmap

  • 2型糖尿病女性患者の自己管理における負担感情と更年期症状の関連について

    笠原 英里子, 青木 萩子, 石田 真由美, 田口 めぐみ, 中村 勝, 林 はるみ, 坂上 百重, サトウ 恵, 堀田 かおり, 山崎 芳裕, 坂井 さゆり, 村松 芳幸

    新潟大学保健学雑誌 = Journal of health sciences of Niigata University   15 ( 1 )   19 - 27   2018年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:新潟大学医学部保健学科  

    壮年期にある2型糖尿病女性患者の自己管理における負担感情と更年期症状との関連を明らかにすることを目的とした。対象者107名,平均年齢58.0歳に厚生労働省栄養摂取状況調査票に基づく質問のほか,糖尿病問題領域質問表(PAID),クッパーマン更年期障害指数安部変法(KKSI)による自記式アンケート調査を実施した。その結果,糖尿病に対する負担感情は更年期症状と弱い正の相関関係を示し,更年期症状の「ゆううつ」はやや強い正の相関関係を示した。更年期前・中・後の3群間で糖尿病に対する負担感情と更年期症状に有意差が認められ,更年期中群は更年期後群と比較し糖尿病に関する負担感情と更年期症状が有意に高かった。糖尿病に対する負担感情を高める影響要因として更年期症状とHbA1c値が推測された。

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: http://hdl.handle.net/10191/49813

  • Taenia solium, Taenia saginata, Taenia asiatica, their hybrids and other helminthic infections occurring in a neglected tropical diseases' highly endemic area in Lao PDR 査読

    Marcello Otake Sato, Megumi Sato, Tetsuya Yanagida, Jitra Waikagul, Tiengkham Pongvongsa, Yasuhito Sako, Surapol Sanguankiat, Tipparayat Yoonuan, Sengchanh Kounnavang, Satoru Kawai, Akira Ito, Munehiro Okamoto, Kazuhiko Moji

    PLoS Neglected Tropical Diseases   12 ( 2 )   e0006260   2018年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science  

    Most part of Southeast Asia is considered endemic for human-infecting Taenia tapeworms
    Taenia solium, T. saginata, and T. asiatica. However, until now there was no report of the occurrence of human cases of T. asiatica in Lao PDR. This study, conducted in Savannakhet Province, Lao PDR, microscopically examined a total of 470 fecal samples by Kato Katz method and found 86% of people harboring at least one helminth. Hookworms were detected in 56% of the samples besides Opisthorchis like eggs (42%), Trichuris trichiura (27%), Ascaris spp. (14%), and Taenia spp. (4%) eggs. Serology for cysticercosis showed 6.8% positives with results varying from 3% to 14.3% in Ethnic School students and Kalouk Kao village respectively. Species-specific PCR targeting mitochondrial DNA (mtDNA) of 28 tapeworms, recovered from 16 patients, revealed T. solium (n = 2), T. saginata (n = 21), and T. asiatica (n = 5). Two patients were confirmed to be coinfected with T. saginata and T. asiatica, indicating the endemicity of the 3 human Taenia in Lao PDR. However, nucleotide sequencing of a nuclear DNA gene, DNA polymerase delta (pold) revealed that all the tapeworms identified as T. asiatica using mtDNA had T. saginata type allele at pold locus, demonstrating that they are not “pure T. asiatica” but the hybrid descendants between the two species, confirming the wide distribution of hybrids of T. saginata/ T. asiatica in Southeast Asia. The high prevalence of several helminthic NTDs in east Savannakhet area even with conventional control measures indicates the importance to establish wide and multifaceted health programs to sustainably improve the quality of life of the populations living in these communities.

    DOI: 10.1371/journal.pntd.0006260

    Scopus

    PubMed

    researchmap

  • LAMPhimerus: A novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples 査読

    William Cevallos, Pedro Fernández-Soto, Manuel Calvopiña, Cristina Fontecha-Cuenca, Hiromu Sugiyama, Megumi Sato, Julio López Abán, Belén Vicente, Antonio Muro

    PLoS Neglected Tropical Diseases   11 ( 6 )   e0005672   2017年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science  

    Background: Amphimeriasis is a fish-borne disease caused by the liver fluke Amphimerus spp. that has recently been reported as endemic in the tropical Pacific side of Ecuador with a high prevalence in humans and domestic animals. The diagnosis is based on the stool examination to identify parasite eggs, but it lacks sensitivity. Additionally, the morphology of the eggs may be confounded with other liver and intestinal flukes. No immunological or molecular methods have been developed to date. New diagnostic techniques for specific and sensitive detection of Amphimerus spp. DNA in clinical samples are needed. Methodology/Principal findings: A LAMP targeting a sequence of the Amphimerus sp. internal transcribed spacer 2 region was designed. Amphimerus sp. DNA was obtained from adult worms recovered from animals and used to optimize the molecular assays. Conventional PCR was performed using outer primers F3-B3 to verify the proper amplification of the Amphimerus sp. DNA target sequence. LAMP was optimized using different reaction mixtures and temperatures, and it was finally set up as LAMPhimerus. The specificity and sensitivity of both PCR and LAMP were evaluated. The detection limit was 1 pg of genomic DNA. Field testing was done using 44 human stool samples collected from localities where fluke is endemic. Twenty-five samples were microscopy positive for Amphimerus sp. eggs detection. In molecular testing, PCR F3-B3 was ineffective when DNA from fecal samples was used. When testing all human stool samples included in our study, the diagnostic parameters for the sensitivity and specificity were calculated for our LAMPhimerus assay, which were 76.67% and 80.77%, respectively. Conclusions/Significance: We have developed and evaluated, for the first time, a specific and sensitive LAMP assay for detecting Amphimerus sp. in human stool samples. The procedure has been named LAMPhimerus method and has the potential to be adapted for field diagnosis and disease surveillance in amphimeriasis-endemic areas. Future large-scale studies will assess the applicability of this novel LAMP assay.

    DOI: 10.1371/journal.pntd.0005672

    Scopus

    PubMed

    researchmap

  • The role of domestic dogs in the transmission of zoonotic helminthes in a rural area of Mekong river basin 査読

    Marcello Otake Sato, Megumi Sato, Tippayarat Yoonuan, Tiengkham Pongvongsa, Surapol Sanguankiat, Sengchanh Kounnavong, Wanna Maipanich, Yuichi Chigusa, Kazuhiko Moji, Jitra Waikagul

    ACTA PARASITOLOGICA   62 ( 2 )   393 - 400   2017年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WALTER DE GRUYTER GMBH  

    Dogs have been bred since ancient times for companionship, hunting, protection, shepherding and other human activities. Some canine helminth parasites can cause significant clinical diseases in humans as Opisthorchis viverrini causing cholangiocarcinoma in Southeast Asian Countries. In this study, socio-cultural questionnaire, canine parasitological analysis, necropsy, parasite molecular confirmation and dog roaming data were evaluated in Savannakhet, Lao-PDR, a typical Mekong Basin area. Dog owners comprised 48.8% of the studied population, with 61.2% owning one dog, 25.1% 2 dogs, 8.5% 3 dogs and 1.8% owning more than 4 dogs. Data from GPS logger attached to dogs showed they walked from 1.4 to 13.3 km per day, covering an area of 3356.38m2 average, with a routine of accessing water sources. Thirteen zoonotic helminth species were observed. Causative agents of visceral and cutaneous larva migrans occurred in 44.1% and 70% of the samples respectively. Spirometra erinaceieuropaei was detected in 44.1% of samples. Importantly, O. viverrini was found in 8.8% of samples. Besides the known importance of dogs in the transmission of Ancylostoma spp., Toxocara spp. and S. erinaceieuropaei, the observed roaming pattern of dogs confirmed it as an important host perpetuating O. viverrini in endemic areas; their routine access to waterbodies may spread O. viverrini eggs in a favorable environment for the fluke development, facilitating the infection of fishes, and consequently infecting humans living in the same ecosystem. Therefore, parasitic NTDs control programs in humans should be done in parallel with parasite control in animals, especially dogs, in the Mekong River basin area.

    DOI: 10.1515/ap-2017-0047

    Web of Science

    PubMed

    researchmap

  • Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples 査読

    Hiroki Hashizume, Megumi Sato, Marcello Otake Sato, Sumire Ikeda, Tippayarat Yoonuan, Surapol Sanguankiat, Tiengkham Pongvongsa, Kazuhiko Moji, Toshifumi Minamoto

    ACTA TROPICA   169   1 - 7   2017年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in Southeast Asian countries. The trematode, Opisthorchis viverrini, is the causative agent of the disease. Accurate and rapid monitoring of O. viverrini is crucial for disease prevention and containment. Therefore, in this study we sought to develop a novel species -specific real-time PCR assay for detecting O. viverrini using environmental DNA (eDNA). The diagnostic sensitivity of the newly developed real-time PCR assay was similar to that of the traditional PCR assay for 50 fecal samples collected in Lao PDR (21 and 19 samples were positive by real-time PCR and traditional PCR, respectively). The efficacy of eDNA analysis and its applicability in the field were tested using a total of 94 environmental water samples collected from 44 sites in Savannakhet, Lao PDR during May and October 2015 and February 2016. O. viverrini eDNA was detected in five samples by real-time PCR, indicating the presence of the fluke in the area and the risk of infection for individuals consuming fish from these water sources. The application of eDNA analysis would facilitate the identification of O. viverrini endemic hotspots and contribute to the ecological control of opisthorchiasis, and this strategy can be applied to other eukaryotic water pathogens. (C)2017 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.actatropica.2017.01.008

    Web of Science

    PubMed

    researchmap

  • First Record of Paramphistomes Fischoederius cobboldi and Paramphistomum epiclitum Detected in Bovine Rumen from a Local Market of Savannakhet Province, Lao PDR 査読

    Surapol Sanguankiat, Marcello Otake Sato, Megumi Sato, Wanna Maipanich, Tippayarat Yoonuan, Tiengkham Pongvongsa, Boungnong Boupha, Yuichi Chigusa, Kazuhiko Moji, Jitra Waikagul

    KOREAN JOURNAL OF PARASITOLOGY   54 ( 4 )   543 - 547   2016年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI  

    In the present study, we report on the occurrence of paramphistomes, Fischoederius cobboldi and Paramphistomum epiclitum, in Lao PDR with the basis of molecular data. Parasite materials were collected from bovines bred in Ban Lahanam area, Savannakhet Province, Lao PDR at Lahanam public market. Morphological observations indicated 2 different species of paramphistomes. The mitochondrial gene cox1 of the specimens was successfully amplified by PCR and DNA sequencing was carried out for diagnosis of 11 specimens. Pairwise alignment of cox1 sequences were performed and confirmed F. cobboldi and P. epiclitum infecting bovines in Laos. Although there were many limiting points, as the small number of worm samples, and the restricted access of the animal host materials, we confirmed for the first time that 2 species of paramphistomes, F. cobboldi and P. epiclitum, are distributed in Lao PDR. More studies are needed to confirm the paramphistome species present in Savannakhet and its hosts to clear the natural history of these parasites of ruminants in the region and measure the impact of this parasite infection in the life and health of the local people.

    DOI: 10.3347/kjp.2016.54.4.543

    Web of Science

    PubMed

    researchmap

  • Morbidity assessment of Opisthorchis viverrini infection in rural Laos: I. Parasitological, clinical, ultrasonographical and biochemical findings 査読

    Hermann Feldmeier, Miklos Hazay, Megumi Sato, Pongvongsa Tiengkham, Futoshi Nishimoto, Hongwei Jiang, Vatsana Sopraseuth, Kazuhiko Moji

    Tropical Medicine and Health   44 ( 1 )   12   2016年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BioMed Central Ltd.  

    Background: Infections with the food-borne trematode Opisthorchis viverinni are common in Southeast Asia. In Lao PDR alone, two million people are supposed to be infected. Opisthorchiasis may cause severe liver disease, eventually leading to cholangiocarcinoma. The objective of this study is to assess the eating habits, complaints, symptoms, signs and ultrasonographical findings in three different areas of Savannakhet Province. Methods: Study participants were recruited in Lahanam village in the flood-prone lowland of, Sonkhone district, Savannakhet Province (group A)
    in Non Somboon village, a community located on a hilly plateau in the same district (group B)
    and in staff of Savannakhet Province Hospital, Savannakhet town (group C). Eating habits, complaints and symptoms were recorded by standardized structured questionnaires. Participants were thoroughly examined clinically, and ultrasonography was performed. O. viverrini eggs were looked for in stool and in duodenal fluid. An array of biochemical and haematological parameters potentially related to liver disease was determined. Group A consisted of 45, group B of 31 and group C of 18 individuals. Results: Eating habits were similar in the three groups, except that participants from group C tended to consume less high-risk types of fish dishes and more frequently ate beef and pork. Average intensity of infection (eggs per gram of stool) was low, but significantly higher in group A than in group B and C (p &lt
    0.001). Medical history and complaints were similar in the three groups. Ultrasonography did not reveal any bile duct pathology. The only pathological finding was a slight elevation of ASAT and gamma-GT in a few participants in groups A and B. Conclusions: The study shows that eating habits favouring the infection with O. viverrini are common in south Laos. Although the average intensity of infection was low, there was a significant difference between the groups, paralleling slightly different eating habits. Clinically, this corresponded to a paucity of liver disease-associated complaints and signs. The low intensity of infection probably explains why no alterations of bile ducts were detectable by ultrasonography.

    DOI: 10.1186/s41182-016-0001-1

    Scopus

    PubMed

    researchmap

  • 新潟県の日本紅斑熱患者発生地域におけるマダニ保有リケッチア群調査

    新井 礼子, 加藤 美和子, 青木 順子, 池田 菫, 田村 務, Otake Sato Marcello, サトウ 恵

    新潟県保健環境科学研究所年報   32   70 - 74   2016年

     詳細を見る

    記述言語:日本語   出版者・発行元:新潟県保健環境科学研究所  

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: http://search.jamas.or.jp/link/ui/2018088550

  • Detection of Plasmodium knowlesi DNA in the urine and faeces of a Japanese macaque (Macaca fuscata) over the course of an experimentally induced infection 査読

    Satoru Kawai, Megumi Sato, Naoko Kato-Hayashi, Hisashi Kishi, Michael A. Huffman, Yoshimasa Maeno, Richard Culleton, Shusuke Nakazawa

    MALARIA JOURNAL   13   373   2014年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Diagnostic techniques based on PCR for the detection of Plasmodium DNA can be highly sensitive and specific. The vast majority of these techniques rely, however, on the invasive sampling of blood from infected hosts. There is, currently, considerable interest in the possibility of using body fluids other than blood as sources of parasite DNA for PCR diagnosis.
    Methods: Urine and faeces were obtained from a Plasmodium knowlesi infected-Japanese macaque (Macaca fuscata) over the course of an experimentally induced infection. P. knowlesi DNA (PkDNA) extracted from urine and faeces were monitored by nested PCR targeting the P. knowlesi specific cytochrome b (cytb) gene.
    Results: Urinary PkDNA was detected on day 2, but was not amplified using DNA templates extracted from the samples on day 4, day 5 and day 6. Subsequently, urinary PkDNA was detected from day 7 until day 11, and from day 20 until day 30. PkDNA in faeces was detected from day 7 until day 11, and from day 20 until day 37. Moreover, real-time quantitative PCR showed a remarkable increase in the amount of urinary PkDNA following anti-malarial treatment. This might have been due to the release of a large amount of PkDNA from the degraded parasites as a result of the anti-malarial treatment, leading to excretion of PkDNA in the urine.
    Conclusions: The cytb-PCR system using urine and faecal samples is of potential use in molecular epidemiological surveys of malaria. In particular, monkey faecal samples could be useful for the detection of zoonotic primate malaria in its natural hosts.

    DOI: 10.1186/1475-2875-13-373

    Web of Science

    PubMed

    researchmap

  • エコヘルスという視点(Vol.6)寄生虫学からみたエコヘルス : タイ肝吸虫を例に

    サトウ 恵, オオタケ・サトウ マルセロ

    医学のあゆみ   249 ( 7 )   630 - 634   2014年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:医歯薬出版  

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: http://search.jamas.or.jp/link/ui/2014214572

  • Patterns of trematode infections of <i>Opisthorchis viverrini</i> (Opisthorchiidae) and <i>Haplorchis taichui</i> (Heterophyidae) in human populations from two villages in Savannakhet Province, Lao PDR 査読

    M. Sato, T. Pongvongsa, S. Sanguankiat, T. Yoonuan, J. Kobayashi, B. Boupha, F. Nishimoto, K. Moji, M.O. Sato, J. Waikagul

    Journal of Helminthology   89 ( 4 )   439 - 445   2014年4月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Cambridge University Press (CUP)  

    Abstract

    The liver fluke, Opisthorchis viverrini, and the minute intestinal fluke, Haplorchis taichui, are prevalent in many Asian countries. This study analysed the patterns of infections of O. viverrini and H. taichui in Lahanam and Thakhamlien villages (Savannakhet Province, Lao PDR), in two cross-sectional investigations. Out of a total of 207 human participants, post-anthelmintic treatment positivity rates for expelled worms were 170 (82.1%) for H. taichui and 65 (31.4%) for O. viverrini. Both these species co-exist in the study villages. When each parasite was analysed separately, H. taichui infections reached a plateau among people aged &gt;20 years. Opisthorchis viverrini infection rates were highest in the age group 21–30 years, with decreasing infection rates after the age of 30. Our findings indicated that fish-borne trematode infections were more prevalent among adults. Fish, common intermediate hosts, were acquired in the study area for analysis. The examination of 35 species of fish as intermediate hosts found O. viverrini metacercariae in only six species, and these were found mostly during the month of November. Many farmers who live on the rice fields obtain their food from their immediate environment, including these intermediate-host fish, potentially putting them at greater risk of O. viverrini infection. By contrast, H. taichui metacercariae were found in three species of fish obtained from the market, meaning that anyone could consume them and become infected. If people who work in rice fields limit the species of fish they consume, or avoid consuming raw fish during the month of November, they may reduce their risk of O. viverrini infection.

    DOI: 10.1017/s0022149x14000261

    researchmap

  • Nematode infection among ruminants in monsoon climate (Ban-Lahanam, Lao PDR) and its role as food-borne zoonosis 査読

    Marcello Otake Sato, Megumi Sato, Kittipong Chaisiri, Wanna Maipanich, Tippayarat Yoonuan, Surapol Sanguankiat, Tiengkham Pongvongsa, Boungnong Boupha, Kazuhiko Moji, Jitra Waikagul

    REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA   23 ( 1 )   80 - 84   2014年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BRAZILIAN COLL VETERINARY PARASITOLOGY  

    Trichostrongylids infection has gained significant public health importance since Trichostrongylus spp. infections have been reported in humans in Lao PDR. In this study, gastrointestinal nematodes were identified and the intensity of infections was determined in goats and cattle, which are animals greatly used for meat production in Lahanam Village, Lao PDR. The total number of goats and bovines was 23 and 29, respectively, pertaining to 32 households surveyed in the area. Feacal samples were randomly collected from 14 goats and 11 bovines. Ninety three percent (13/14) of goats and 36% (3/11) of cattle were infected, with an average of 1,728 and 86 eggs per gram of faeces (EPG), respectively. Coproculture showed Trichostrongylus spp. (goats 16%; bovines 48%), Haemonchus spp. (goats 69%; bovines 37%), Cooperia spp. (bovines 8%) and Oesophagostomum spp. (goats 15%; bovines 6%). After performing the necropsy on an adult goat, Trichuris spp. was also found. We confirmed the presence of Oesophagostomum spp., H. contortus and T. colubriformis by morphology and DNA sequencing analysis of the ITS region of rDNA. Due to interactions between humans and goats in Lahanam Village and high EPG results, the diagnosis of species and the intensity of gastrointestinal nematode infection in these animals are important public-health issues. Other ruminant parasites, such as Oesophagostomum and Haemonchus, found in caprines and bovines, are reported to be causes of zoonosis and their presence in humans should be investigated in future field surveys in this area.

    DOI: 10.1590/S1984-29612014011

    Web of Science

    researchmap

  • Prevalence and clinical aspects of human Trichostrongylus colubriformis infection in Lao PDR 査読

    Dorn Watthanakulpanich, Tiengkham Pongvongsa, Surapol Sanguankiat, Supaporn Nuamtanong, Wanna Maipanich, Tippayarat Yoonuan, Orawan Phuphisut, Boungnong Boupha, Kazuhiko Moji, Megumi Sato, Jitra Waikagul

    Acta Tropica   126 ( 1 )   37 - 42   2013年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    There have been few studies on human trichostrongyliasis in Southeast Asia, information on its clinical manifestations is also sparse. Trichostrongyliasis occurs predominantly in areas where poor hygiene is common especially where human/animal feces are used as a fertilizer, thereby contaminating vegetables and stream water. The intimate coexistence of domestic animals and humans explains the prevalence of Trichostrongylus infection in such areas. The goal of the current study was to determine the prevalence of trichostrongyliasis among villagers in Thakamrien village, Sonkon district, Savannakhet province, Laos, and to investigate potential relationships between clinical features, laboratory data, and severity of infection. Of 272 villagers examined, 160 (58.8%) were determined positive for helminthic infections by fecal examination, and 59 (36.9%) of these were infected with Trichostrongylus. Only 58 cases were in the inclusion criteria of the study and then underwent further assessment, including a questionnaire on personal behaviors, physical examination, and laboratory tests. Villagers in the trichostrongyliasis group were more likely than the control group to have consumed fresh vegetables, not washed their hands before meals or after using the toilet, and to have had close contact with herbivorous animals (goats and cows). Similarly, villagers in the trichostrongyliasis group were more likely than the control group to have a history of loose feces, rash, or abdominal pain
    however, no obvious clinical symptoms were observed during physical examination of the trichostrongyliasis patients. The degree of infection was determined by both fecal egg counts and quantification of adult worms after deworming. Laboratory data were evaluated for any relationship with severity of infection. No significant differences were found in laboratory values between the trichostrongyliasis and control groups, with most values being within normal limits
    however, both groups had high eosinophil counts. This study demonstrated that the useful clinical characteristics of trichostrongyliasis patients include history of loose feces, rashes, and abdominal pain, as well as in personal behaviors, such as the regular consumption of fresh vegetables, lack of hand washing, and close contact with cattle. © 2013 Elsevier B.V.

    DOI: 10.1016/j.actatropica.2013.01.002

    Scopus

    PubMed

    researchmap

  • ABNORMAL TAENIA SAGINATA TAPEWORMS IN THAILAND 査読

    Wanna Maipanich, Megumi Sato, Somchit Pubampen, Surapol Sanguankiat, Teera Kusolsuk, Urusa Thaenkham, Jitra Waikagul

    SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH   42 ( 5 )   1065 - 1071   2011年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOUTHEAST ASIAN MINISTERS EDUC ORGANIZATION  

    Sixty-eight residents of Ban Luang and Ban Pang Kae villages, in Nan Province, northern Thailand, visited our mobile field station in September 2006 and March 2007, seeking treatment for taeniasis. After treatment, 22 cases discharged tapeworm strobila in their fecal samples and 17 scolices were recovered. Among these, 3 were morphologically abnormal, with six suckers on the scolex. To confirm the species of these tapeworms, the mitochondrial cytochrome c oxidase subunit I (COI) gene was used as a molecular marker. The partial COI sequences (800 bp) of the abnormal tapeworms were identical to the sequences of Taenia saginata deposited in Genbank.

    Web of Science

    PubMed

    researchmap

  • Short Report Human Trichostrongylus colubriformis Infection in a Rural Village in Laos 査読

    Megumi Sato, Tippayarat Yoonuan, Surapol Sanguankiat, Supaporn Nuamtanong, Tiengkham Pongvongsa, Inthava Phimmayoi, Vilayphone Phanhanan, Boungnong Boupha, Kazuhiko Moji, Jitra Waikagul

    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE   84 ( 1 )   52 - 54   2011年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC TROP MED & HYGIENE  

    In Lahanam Village, Savannakhet Province, Laos, 125 of 253 villagers (49 w4%) were found by fecal examination to harbor hookworm eggs The eggs were heterogeneous in morphology and size, suggesting infections of mixed nematode species To confirm the hookworm egg species, on a voluntary basis, 46 hookworm egg-positive participants were treated with albendazole, and post-treatment adult worms were collected from purged fecal samples The common human hookworm was found in only 3 participants, 1 case of Necator americanus, and 2 cases of Ancylostoma duodenale In contrast, adult Trichostrongylus worms were expelled from most participants (43 of 46, 93 5%) The Trichostrongylus species were confirmed by morphology and internal transcribed spacer 2 sequences, all worms were of the same species (T colubriformis) In addition, some Trichostrongylus worms were obtained from a goat in the same village and identified as T colubriformis The results suggested that T colubriformis was the main zoonotic species causing hookworm infections in the village

    DOI: 10.4269/ajtmh.2011.10-0385

    Web of Science

    PubMed

    researchmap

  • Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand 査読

    Malinee T. Anantaphruti, Munehiro Okamoto, Tippayarat Yoonuan, Surapol Saguankiat, Teera Kusolsuk, Megumi Sato, Marcello O. Sato, Yasuhito Sako, Jitra Waikagul, Akira Ito

    PARASITOLOGY INTERNATIONAL   59 ( 3 )   326 - 330   2010年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    A community-based field survey on taeniasis and cysticercosis was performed in two villages in Thong Pha Phum District, Kanchanaburi Province, central Thailand, where 3 Taenia species, T. solium, T. saginata and T. asiatica, are sympatrically occurring. Four (0.6%) out of 667 stool samples were egg-positive for Taenia sp. by Kato-Katz technique. Three out of those four persons and other three persons who were Taenia egg-negative but having a recent (&lt;1 year) history of discharging worms in stool were treated with niclosamide. One Taenia egg-positive woman was not treated because of severe ascites. After treatment, three persons expelled long strobilae with scolices and two persons expelled strobilae without scolex. One Taenia egg-positive person did not expel any worms post-treatment. Among 5 persons, four expelled a single worm, whereas one expelled multiple worms, may be 6 worms but not confirmed by detection of scolices. One scolex was armed with hooklets, whereas 2 others did not. Multiplex PCR of 10 expelled proglottids (including 6 estimated worms from one patient) revealed that one sample was T. solium, one T. saginata, and 8 T. asiatica. A total of 159 residents agreed to receive a serological test for cysticercosis. By ELISA using partially purified glycoprotein antigen, 9 cases, 5 and 4 from villages A and B respectively, were found to be sero-positive. The five and an additional sample on the border line from village A were evaluated using confirmative immunoblot using recombinant chimeric antigen. Among the six samples, four including the border line sample were confirmed to be cysticercosis by immunoblotting. One of the 4 persons had neurological symptoms with nodular lesions in the brain by computed tomography. These 4 confirmed or suspected cysticercosis cases were free of T. solium worms, but two of them including confirmed NCC case had a past (&gt;1 year) history of expelling proglottids in the stool. (c) 2010 Published by Elsevier Ireland Ltd.

    DOI: 10.1016/j.parint.2010.03.007

    Web of Science

    PubMed

    researchmap

  • Systematics of the subfamily Haplorchiinae (Trematoda: Heterphyidae), based on nuclear ribosomal DNA genes and ITS2 region 査読

    Urusa Thaenkham, Paron Dekumyoy, Chalit Komalamisra, Megumi Sato, Do Trung Dung, Jitra Waikagul

    PARASITOLOGY INTERNATIONAL   59 ( 3 )   460 - 465   2010年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Phylogenetic relationships of 6 species in the trematode subfamily Haplorchiinae were analyzed using small and large subunit of ribosomal DNA genes (185 rDNA and 28S rDNA) and internal transcribed spacer subunit II (ITS2) region as molecular markers. Maximum Likelihood and Bayesian inference analyses of combined rDNAs and ITS2 indicated a close relationship between the genera Haplorchis and Procerovum, while these two genera were distinct from Stellantchasmus falcatus. These phylogenetic relationships were consistent with the number of testes but not with the characters of the modification of the seminal vesicle or of the ventral sucker. Although three Haplorchis spp. were, together with Procerovum, in the same cluster, their mutual topology was incongruent between rDNA and ITS2 trees. Phylogenetic analyses using other molecular markers with more species are necessary to work out solid phylogenetic relationships among the species in this subfamily. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.parint.2010.06.009

    Web of Science

    PubMed

    researchmap

  • Copro-molecular identification of infections with hookworm eggs in rural Lao PDR 査読

    Megumi Sato, Surapol Sanguankiat, Tippayarat Yoonuan, Tiengkham Pongvongsa, Malaythong Keomoungkhoun, Inthava Phimmayoi, Boungnong Boupa, Kazuhiko Moji, Jitra Waikagul

    TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE   104 ( 9 )   617 - 622   2010年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The prevalence of hookworm eggs in fecal samples, by modified cellophane thick-smear technique, was found to be 30.0% (61/203 samples) in Lahanam Village, Savannakhet Province, Lao PDR. The hookworm eggs were morphologically heterogeneous, so that identification was confirmed by copro-PCR with specific primers for hookworms and Trichostrongylus spp.; 12 samples were positive for Necator americanus, 19 for Ancylostoma spp., with one mixed infection of both. Sequencing of the Ancylostoma spp. copro-PCR products found A. duodenale, and also the animal hookworms, A. caninum and A. ceylanicum. Moreover, Trichostrongylus spp.-specific copro-PCR revealed a significant rate of infection (43/203; 21.2%). Sequencing confirmed the zoonotic species, T. colubriformis. PCR/sequencing is useful for differentiating parasite species. The positives by the Kato-Katz method, and by the combined hookworm/Trichostrongylus specific-PCR, were comparable, although the agreement between the two methodologies was only 50%. (c) 2010 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.trstmh.2010.06.006

    Web of Science

    PubMed

    researchmap

  • Monophyly of Opisthorchis viverrini populations in the lower Mekong Basin, using mitochondrial DNA nad1 gene as the marker 査読

    Urusa Thaenkham, Supaporn Nuamtanong, Surapol Sa-nguankiat, Tippayarat Yoonuan, Sarun Touch, Khemphavanh Manivong, Youthanavanh Vonghachack, Megumi Sato, Jitra Waikagul

    PARASITOLOGY INTERNATIONAL   59 ( 2 )   242 - 247   2010年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    The liver fluke. Opisthorchis viverrini, causes serious public-health problems in the Lower Mekong Basin. This study aimed to clarify whether O. viverrini populations may be genetically divided into sub-specific taxa. We collected 6 populations of O. viverrini from different places in Cambodia, Lao PDR, and Thailand, along both sides of the Mekong River, and analyzed the population structure of these using the mitochondrial nad1 gene as a marker. The results of the DNA polymorphism measurements, by theta-w (theta w) and -pi (theta pi) values, neutrality tests, and mismatch distribution, suggested that the population of O. viverrini has expanded under the influence of purifying selection and selective sweep. The analysis of molecular variance (AMOVA) test revealed no significant genetic differences among the O. viverrini populations on opposite sides of the Mekong River. O. viverrini haplotypes occurred in multiple populations, and no distinct geographical clade. The star-like haplotype network confirmed a demographic expansion of the O. viverrini population. Overall, the genetic data from these populations suggested that the postulated existence of an O. viverrini species complex should be rejected. The bio-geographical diversity of O. viverrini populations should be explored further, using other appropriate markers and a wider range of samples from geographically different areas. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.parint.2010.02.009

    Web of Science

    PubMed

    researchmap

  • COPRO-DNA DIAGNOSIS OF OPISTHORCHIS VIVERRINI AND HAPLORCHIS TAICHUI INFECTION IN AN ENDEMIC AREA OF LAO PDR 査読

    Megumi Sato, Tiengkham Pongvongsa, Surapol Sanguankiat, Tipparayat Yoonuan, Paron Dekumyoy, Thareerat Kalambaheti, Malaythong Keomoungkhoun, Inthava Phimmayoi, Boungnong Boupha, Kazuhiko Moji, Jitra Waikagul

    SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH   41 ( 1 )   28 - 35   2010年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOUTHEAST ASIAN MINISTERS EDUC ORGANIZATION  

    The utility of differential copro-DNA diagnosis using modified sample preparation steps of small liver and minute intestinal fluke infections was tested. Fecal samples containing parasite eggs were washed extensively with diluted detergent Solution. Parasite eggs were concentrated by sedimentation and broken by microwaving before DNA extraction. PCR targeting ITS1 and ITS2 regions were performed using primer specific for Opisthorchis viverrini, Haplorchis taichui and other related species. Of 125 fecal samples, 94 were positive for small trematode eggs by a modified cellophane thick smear method. By ITS1-PCR, 52 samples were positive for O. viverrini, 12 H. taichui and 7 mixed infection. By ITS2-PCR, 63 were positive for O. viverrini, 17 H. taichui, and 19 mixed infection. The ITS-PCR assay identified a higher number of opisthorchiasis cases than those with O. viverrini expelled after treatment, but for H. taichui, ITS-PCR identified less than half of the worm expelled cases. These results showed that copro-DNA diagnosis was useful for the differential diagnosis of O. viverrini and H. taichui infection, which could not be discriminated by microscopy.

    Web of Science

    PubMed

    researchmap

  • Egg Laying Capacity of Haplorchis taichui (Digenea: Heterophyidae) in Humans 査読

    Megumi Sato, Surapol Sanguankiat, Somchit Pubampen, Teera Kusolsuk, Wanna Maipanich, Jitra Waikagul

    KOREAN JOURNAL OF PARASITOLOGY   47 ( 3 )   315 - 318   2009年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI  

    Quantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui ( Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.

    DOI: 10.3347/kjp.2009.47.3.315

    Web of Science

    PubMed

    researchmap

  • Discrimination of O. viverrini, C. sinensis, H. pumilio and H. taichui using nuclear DNA-based PCR targeting ribosomal DNA ITS regions 査読

    Megumi Sato, Urusa Thaenkham, Paron Dekumyoy, Jitra Waikagul

    ACTA TROPICA   109 ( 1 )   81 - 83   2009年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Small liver and minute intestinal flukes are highly prevalent in Southeast Asia, and in mixed infections, their eggs are difficult to differentiate morphologically in fecal samples. PCR assays targeting the ITS regions in ribosomal DNA were designed to identify and differentiate species. The PCR amplicons of Opisthorchis viverrini, Clonorchis sinensis, Haplorchis pumilio, and Haplorchis taichui were 800, 820, 1250, and 930 bp for the ITS1 region, and 380,390,380, and 530 bp for ITS2, respectively. The ITS1-region amplicon sizes successfully differentiated 4 species, while only H. taichui were significantly different from the other 3 species in the ITS2 region. PCR assays were employed for preliminary analysis using fecal samples diagnosed as having "small trematode eggs" by modified thick smear, showing 76.2% sensitivity for ITS1 and 95.2% for ITS2. (C) 2008 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.actatropica.2008.09.015

    Web of Science

    PubMed

    researchmap

  • Enterobiasis: a neglected infection in adults. 査読

    Megumi SATO

    The Southeast Asian journal of tropical medicine and public health   39 ( 2 )   213 - 216   2008年3月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    In this study, adult patients were treated with praziquantel to expel intestinal flukes. Unexpectedly, dozens of adult Enterobius vermicularis worms with disfigured morphology, which had not been detected on fecal examination using Kat's modified thick-smear technique, were expelled from 6 of 33 patients.

    PubMed

    researchmap

▶ 全件表示

書籍等出版物

  • クエスチョン・バンク 臨床検査技師2023

    ( 担当: 分担執筆 ,  範囲: 寄生虫)

    2022年5月  ( ISBN:9784896328578

     詳細を見る

  • Climate, Ticks and Disease

    ( 担当: 共著 ,  範囲: Chapter 51: The expansion of Japanese spotted fever and the complex group of spotted fever group rickettsia in Japan)

    CABI  2021年11月 

     詳細を見る

  • Zoonotic Diseases and One Health

    ( 担当: 共著)

    MDPI Books  2020年5月 

     詳細を見る

  • Biology of Foodborne Parasites

    サトウ 恵( 担当: 分担執筆 ,  範囲: Chapter 24. Taenia)

    CRC Press  2015年4月 

     詳細を見る

  • Foodborne Parasites in the Food Supply Web

    サトウ 恵( 担当: 分担執筆 ,  範囲: Chapter 10 - Foodborne parasites)

    Elsevier  2015年4月 

     詳細を見る

MISC

  • 将来の妊娠に向けたからだづくりをめざした公開講座『将来子どもが欲しいあなたへ』の実践報告

    林 はるみ, 石田 真由美, 坂上 百重, サトウ 恵, 高橋 俊博, 池主 雅臣, 中村 勝, 西方 真弓, 西原 亜矢子, 堀田 かほり, 山崎 芳裕, 青木 萩子

    日本不妊カウンセリング学会誌   15 ( 1 )   70 - 71   2016年4月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本不妊カウンセリング学会  

    researchmap

  • 環境中水でタイ肝吸虫Opisthorchis viverrini DNAを検出するための環境DNA手法の開発

    サトウ恵, 橋爪裕宜, SATO OTAKE Marcello, YOONUAN Tippayarat, SURAPOL Sanguankiat, 池田菫, PONGVONGSA Tiengkham, 門司和彦, 源利文

    日本熱帯医学会大会プログラム抄録集   57th   2016年

     詳細を見る

  • 環境DNA手法の感染症生態学への応用:タイ肝吸虫Opisthorchis viverriniの検出

    橋爪裕宜, サトウ恵, SATO Marcello Otake, YOONUAN T., SANGUANKIAT S., PONGVONGSA T., 門司和彦, 源利文

    日本生態学会大会講演要旨(Web)   63rd   2016年

     詳細を見る

  • Detection of Extended Spectrum β-Lactamase producing Escherichia coli (ESBL E.coli) from chicken meat in Niigata Prefecture, Japan

    BANDARA Holipitige Pubuduni Sugandhika, SATO Marcello Otake, SATO Megumi, Yatawara Lalani, Watanabe Kanako

    新潟大学保健学雑誌 = Journal of health sciences of Niigata University   12 ( 1 )   65 - 68   2015年9月

     詳細を見る

    記述言語:英語   出版者・発行元:新潟大学医学部保健学科  

    The extended-spectrum β-lactamases (ESBLs) are the enzymes which degrade oxyimino-cephalosporins such as cefotaxime and ceftazidime, and make the antibiotics ineffective. In the past decade, drug resistance derived from Extended-spectrum β-lactamase-producing Escherichia coli (ESBL E. coli) has been increasing dramatically worldwide. The ESBLs genes are located on plasmids that can be easily transferred between and within bacterial species. It is indicated the linkage of ESBL E. coli from the food producing meats and the clinical samples. The presence of ESBL genes has been clearly documented in chickens. In this study, 27 raw chicken livers acquired from a supermarket in Niigata were used for detecting ESBL E. coli. E. coli was identified by DHL and chromogenic agar results as 19 of 27 (70.3%) samples. Antibiotic susceptibility test identified 6 of 19 (31.5%) ampicillin resistant E. coli samples and 1 of 19 (5.2%) ESBL E. coli sample. With genotyping result, an ESBL E. coli isolated in this study was confirmed as CTXM2.

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: http://hdl.handle.net/10191/38952

  • 途上国におけるDALY計算の値をどう考えるか? : タイ肝吸虫症の事例から (日本環境共生学会第16回(2013年度)学術大会発表論文集) -- (環境情報を活用した都市政策検討の新たな視点 : QOL指標が結ぶ防災,健康,そして住環境の評価)

    蒋 宏偉, 門司 和彦, サトウ 恵

    日本環境共生学会学術大会発表論文集   354 - 357   2013年

     詳細を見る

    記述言語:日本語   出版者・発行元:日本環境共生学会  

    CiNii Article

    researchmap

受賞

  • 日本熱帯医学会女性賞

    2023年10月   日本熱帯医学会  

     詳細を見る

  • 2nd Best Poster Award

    2016年10月   ラオス国立公衆衛生研究所  

    サトウ 恵

     詳細を見る

  • 日本熱帯医学会研究奨励賞

    2013年10月   日本熱帯医学会  

    サトウ 恵

     詳細を見る

共同研究・競争的資金等の研究

  • 環境DNA手法を利用したタイ肝吸虫撲滅を目指したワンヘルス・エコヘルス対策

    研究課題/領域番号:24K03089

    2024年4月 - 2027年3月

    制度名:科学研究費助成事業

    研究種目:基盤研究(B)

    提供機関:日本学術振興会

    サトウ 恵

      詳細を見る

    配分額:18590000円 ( 直接経費:14300000円 、 間接経費:4290000円 )

    researchmap

  • メコン住血吸虫の撲滅に向けた多面的な融合対策プログラム作成と数理モデルによる感染リスク分析による「最後の一押し!」

    2024年2月 - 2027年1月

    制度名:令和5年度 「医療分野国際科学技術共同研究開発推進事業(戦略的国際共同研究プログラム SICORP)e-ASIA共同研究プログラム

    提供機関:国立研究開発法人日本医療研究開発機構

      詳細を見る

    担当区分:研究代表者 

    researchmap

  • 革新的技術を活用したマラリア及び顧みられない寄生虫症の制圧と排除に関する研究開発

    2023年4月 - 2028年3月

    制度名:「地球規模課題対応国際科学技術協力プログラム(SATREPS)」

    提供機関:国立研究開発法人日本医療研究開発機構

    石上 盛敏

      詳細を見る

    担当区分:研究分担者 

    researchmap

  • アジアの住血吸虫症撲滅を目指した革新的ポイント・オブ・ケア検査法ならびに環境汚染監視ツールの開発

    2022年1月

    制度名:令和3年度「医療分野国際科学技術共同研究開発推進事業 戦略的国際共同研究プログラム(SICORP)e-ASIA共同研究プログラム」

    提供機関:国立研究開発法人日本医療研究開発機構(AMED)

    迫 康仁、オオタケサトウ マルセロ、サトウ恵

      詳細を見る

    担当区分:研究分担者 

    researchmap

  • 土壌環境DNA解析に基づく土壌伝播蠕虫感染症ダイナミクスの解明

    研究課題/領域番号:21K12269

    2021年4月 - 2024年3月

    制度名:科学研究費助成事業 基盤研究(C)

    研究種目:基盤研究(C)

    提供機関:日本学術振興会

    オオタケサトウ マルセロ

      詳細を見る

    配分額:3900000円 ( 直接経費:3000000円 、 間接経費:900000円 )

    Goal: Development of soil transmitted helminths-specific DNA detection method.
    <BR>
    The DNA sequences of the parasite species causing STH were retrieved from the database, aligned and analyzed. We could obtain sequences from the following species: Ascaris lumbricoides, Trichuris trichiura and Hookworms (Ancylostoma duodenale, Ancylostoma ceylanicum, Necator americanus). Moreover, other species DNA sequences were also retrieved, aligned, and analysed once they are also found in STH endemic areas, namely: Ascaris suum, Toxocara canis, Toxocara cati, Ancylostoma caninum, Ancylostoma braziliense.
    To design the environmental DNA detection assay for soil samples, we choose mitochondrial and nuclear genes. The target genes were mitochondrial DNA 16S rRNA, COX1 gene, or nuclear DNA ITS1, ITS2, which are often used to distinguish parasite species. From the aligned sequences of each gene by species of parasite we could determine intraspecific and interspecific variable and conserved areas which allowed us to design species specific sets of primers and probes with an expected short length of the amplified DNA (about 100 to 200 bp). All the obtained species-specific assays were screened in silico for specificity. At least 3 sets of primers-probe for each STH species were chosen to proceed to sensitivity and interspecies specificity tests.

    researchmap

  • 環境DNAを用いたタイ肝吸虫の感染リスク評価

    研究課題/領域番号:20H04346

    2020年4月 - 2023年3月

    制度名:科学研究費助成事業 基盤研究(B)

    研究種目:基盤研究(B)

    提供機関:日本学術振興会

    サトウ 恵, 源 利文, オオタケサトウ マルセロ

      詳細を見る

    配分額:17680000円 ( 直接経費:13600000円 、 間接経費:4080000円 )

    researchmap

  • An integrative diagnostic approach in detecting human and animal schistosomiasis in situations of varying levels of prevalence in the Philippines

    2020年 - 2021年

    制度名:Joint TDR/WPRO Small Grants Scheme for Implementation Research in Infectious Diseases of Poverty

    提供機関:WHO

    Raffy Jay, C. Fornillos Ian Kendrich Fontanilla, Lydia Leonardo, Winston Palasi, Ian Kim Tabios, Marcello Otake Sato, Megumi Sato, Carlos Graeff Teixeira

      詳細を見る

    担当区分:研究分担者 

    researchmap

  • マダニ生息分布調査と次世代シーケンシングによる保有病原体の網羅的検出

    研究課題/領域番号:16K00569

    2016年4月 - 2019年3月

    制度名:科学研究費助成事業 基盤研究(C)

    研究種目:基盤研究(C)

    提供機関:日本学術振興会

    サトウ 恵, 渡辺 幸三, 中尾 稔

      詳細を見る

    配分額:4810000円 ( 直接経費:3700000円 、 間接経費:1110000円 )

    2016年から2018年度の3年間で新潟県下においてマダニの採取を行った。マダニ種の分類を行い、それぞれの地域において現在分布しているマダニ種のおおよその把握を行うことができた。新潟県で現在確認されたマダニ種は14種で、1950年代には見られなかった南方系のマダニ種も確認している。またマダニが保有しているリケッチア、ボレリアの検出を行い、それぞれに病原
    体の種特異性を確認した。検出されたリケッチアはR. asiatica, R. helvetica, R. monacensis の3種であった。ボレリアはB. japonica,B. miyamotoiまた、種不明Borrelia属が検出された。

    researchmap

  • 沖縄感染症研究拠点形成促進プロジェクト

    2015年9月 - 2018年3月

    制度名:沖縄感染症研究成果活用促進事業

    小林潤

      詳細を見る

    担当区分:研究分担者 

    researchmap

  • 森林発生人獣共通マラリアの生態学社会学的研究

    研究課題/領域番号:23406022

    2011年4月 - 2014年3月

    制度名:科学研究費助成事業 基盤研究(B)

    研究種目:基盤研究(B)

    提供機関:日本学術振興会

    中澤 秀介, カレトン リチャード, 益田 岳, 前野 芳正, 川合 覚, サトウ 恵, 小林 繁男, 金子 修, 湯本 湯本, ハフマン マイケル, M・A Huffman

      詳細を見る

    配分額:19110000円 ( 直接経費:14700000円 、 間接経費:4410000円 )

    ベトナム・カンホア省カンフー地区の住民、ベトナム・クワンチ省ラオスサバナケット県間の国境地域の住民の血液からサルマラリア原虫Plasmodium knowlesiを検出した。
    カンフー地区で採取したハマダラカAnopheles dirusからヒトマラリア原虫3種とサルマラリア原虫4種を検出した。P. knowlesi感染実験用サルの糞便からそのDNAを検出した。野生サルにおいても同様にP. knowlesiのDNAを検出した。P. knowlesi感染住民の血液中に生殖母体を検出した。ヒトマラリア伝播が特定グループで維持される傾向があった。地形植生、土地利用、住民の生活基盤が明らかになった。

    researchmap

▶ 全件表示

 

担当経験のある授業科目

  • ゲノム・微生物病態検査科学特講

    2021年
    -
    現在
    機関名:新潟大学

  • 保健学特定研究(検査技術科学)

    2021年
    -
    現在
    機関名:新潟大学

  • ゲノム・微生物病態検査科学特講演習

    2021年
    -
    現在
    機関名:新潟大学

  • 生活習慣と健康

    2021年
    -
    現在
    機関名:新潟大学

  • 保健学特別研究(検査技術科学)

    2021年
    機関名:新潟大学

  • スタディスキルズ (検査)

    2020年
    -
    現在
    機関名:新潟大学

  • 保健学総合

    2020年
    -
    現在
    機関名:新潟大学

  • 医療英語ベーシック(検査)

    2020年
    機関名:新潟大学

  • 一般検査科学

    2019年
    -
    現在
    機関名:新潟大学

  • 一般検査科学実習

    2018年
    -
    現在
    機関名:新潟大学

  • 医療安全管理学

    2018年
    機関名:新潟大学

  • 入門医療英語

    2018年
    機関名:新潟大学

  • 保健学特別研究(検査技術科学)

    2017年
    -
    2021年
    機関名:新潟大学

  • ゲノム・微生物病態検査学特講演習

    2017年
    機関名:新潟大学

  • ゲノム・微生物病態検査学特講

    2017年
    機関名:新潟大学

  • 臨床検査実習

    2016年
    -
    現在
    機関名:新潟大学

  • 医学検査管理総論

    2016年
    -
    現在
    機関名:新潟大学

  • 環日本海医療概論

    2015年
    -
    2018年
    機関名:新潟大学

  • 情報科学概論演習

    2014年
    機関名:新潟大学

  • 寄生虫検査科学

    2013年
    -
    現在
    機関名:新潟大学

  • 医療英語(検査)

    2013年
    -
    現在
    機関名:新潟大学

  • 卒業研究

    2013年
    -
    現在
    機関名:新潟大学

  • 病原微生物解析学実習

    2013年
    -
    現在
    機関名:新潟大学

  • 国際病態検査科学概論

    2013年
    -
    現在
    機関名:新潟大学

  • 病原微生物解析学特論

    2013年
    -
    現在
    機関名:新潟大学

  • 寄生虫検査科学実習

    2013年
    -
    現在
    機関名:新潟大学

  • 病原微生物学実習

    2013年
    -
    2020年
    機関名:新潟大学

  • 臨床検査管理概論

    2013年
    -
    2015年
    機関名:新潟大学

  • 微生物検査科学演習

    2013年
    -
    2014年
    機関名:新潟大学

▶ 全件表示