2021/10/11 更新

写真a

カワサキ アサミ
河嵜 麻実
KAWASAKI Asami
所属
教育研究院 医歯学系 特任講師
医歯学総合研究科 特任講師
職名
特任講師
外部リンク

学位

  • 博士(薬学) ( 2009年3月   広島大学 )

  • 修士(薬学) ( 2006年3月   広島大学 )

研究キーワード

  • 包括脳ネットワーク

研究分野

  • ライフサイエンス / 神経科学一般  / 神経回路形成、成長円錐

  • ライフサイエンス / 神経科学一般  / 神経回路形成、成長円錐

経歴(researchmap)

  • 新潟大学 医学部 生化学第二   助教

    2011年4月 - 現在

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  • 大阪大学 医学系研究科 分子生体情報学   博士研究員

    2009年10月 - 2011年4月

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経歴

  • 新潟大学   医歯学総合研究科   特任講師

    2021年4月 - 現在

  • 新潟大学   医歯学総合研究科   特任講師

    2019年4月 - 現在

  • 新潟大学   教育研究院 医歯学系   特任講師

    2019年4月 - 現在

  • 新潟大学   医歯学総合研究科   特任助教

    2017年4月 - 2019年3月

  • 新潟大学   研究推進機構 超域学術院   助教

    2011年4月 - 2017年3月

学歴

  • 広島大学   医歯薬学総合研究科   博士課程

    2004年3月 - 2009年9月

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  • 広島大学   医学部   薬学科

    2000年3月 - 2004年4月

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所属学協会

取得資格

  • 薬剤師

 

論文

  • Growth Cone Phosphoproteomics Reveals that GAP-43 Phosphorylated by JNK Is a Marker of Axon Growth and Regeneration. 査読 国際誌

    Asami Kawasaki, Masayasu Okada, Atsushi Tamada, Shujiro Okuda, Motohiro Nozumi, Yasuyuki Ito, Daiki Kobayashi, Tokiwa Yamasaki, Ryo Yokoyama, Takeshi Shibata, Hiroshi Nishina, Yutaka Yoshida, Yukihiko Fujii, Kosei Takeuchi, Michihiro Igarashi

    iScience   4   190 - 203   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Neuronal growth cones are essential for nerve growth and regeneration, as well as for the formation and rearrangement of the neural network. To elucidate phosphorylation-dependent signaling pathways and establish useful molecular markers for axon growth and regeneration, we performed a phosphoproteomics study of mammalian growth cones, which identified >30,000 phosphopeptides of ∼1,200 proteins. The phosphorylation sites were highly proline directed and primarily MAPK dependent, owing to the activation of JNK, suggesting that proteins that undergo proline-directed phosphorylation mediate nerve growth in the mammalian brain. Bioinformatics analysis revealed that phosphoproteins were enriched in microtubules and the cortical cytoskeleton. The most frequently phosphorylated site was S96 of GAP-43 (growth-associated protein 43-kDa), a vertebrate-specific protein involved in axon growth. This previously uncharacterized phosphorylation site was JNK dependent. S96 phosphorylation was specifically detected in growing and regenerating axons as the most frequent target of JNK signaling; thus it represents a promising new molecular marker for mammalian axonal growth and regeneration.

    DOI: 10.1016/j.isci.2018.05.019

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  • Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis 査読

    Nozomu Yoshioka, Shinji Miyata, Atsushi Tamada, Yumi Watanabe, Asami Kawasaki, Hiroshi Kitagawa, Keizo Takao, Tsuyoshi Miyakawa, Kosei Takeuchi, Michihiro Igarashi

    MOLECULAR BRAIN   10 ( 1 )   47   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BMC  

    Chondroitin sulfate (CS) is an important glycosaminoglycan and is mainly found in the extracellular matrix as CS proteoglycans. In the brain, CS proteoglycans are highly concentrated in perineuronal nets (PNNs), which surround synapses and modulate their functions. To investigate the importance of CS, we produced and precisely examined mice that were deficient in the CS synthesizing enzyme, CSGalNAcT1 (T1KO). Biochemical analysis of T1KO revealed that loss of this enzyme reduced the amount of CS by approximately 50% in various brain regions. The amount of CS in PNNs was also diminished in T1KO compared to wild-type mice, although the amount of a major CS proteoglycan core protein, aggrecan, was not changed. In T1KO, we observed abnormalities in several behavioral tests, including the open-field test, acoustic startle response, and social preference. These results suggest that T1 is important for plasticity, probably due to regulation of CS-dependent PNNs, and that T1KO is a good model for investigation of PNNs.

    DOI: 10.1186/s13041-017-0328-5

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  • Involvement of IQGAP family proteins in the regulation of mammalian cell cytokinesis 査読

    Makoto Adachi, Asami Kawasaki, Hisashi Nojima, Eisuke Nishida, Sachiko Tsukita

    GENES TO CELLS   19 ( 11 )   803 - 820   2014年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    IQGAP family proteins, comprising IQGAP1, -2, and -3 in mammals, are involved in diverse ranges of cellular processes such as adhesion and migration. IQGAP proteins in yeast also play important roles in cytokinesis. However, the involvement of IQGAP proteins in cytokinesis in mammals remains unaddressed. In this study, we showed that IQGAP3 specifically localized to the equatorial cortex at anaphase, whereas IQGAP1 localized to the cell cortex uniformly and IQGAP2 was unexpressed in HeLa cells. IQGAP3, but neither IQGAP1 nor -2, was able to interact with anillin, which was required for the localization of IQGAP3 to the contractile ring. The suppressed expression of IQGAP3 inhibited the completion of cleavage furrow ingression and led to the multinucleation of cells. The suppression of IQGAP1 also had similar inhibitory effects on cytokinesis, and the simultaneous suppression of IQGAP1 and -3 induced more severe effects. The localization of anillin and RhoA to the contractile ring was impaired by the suppression of IQGAP1 and -3, whereas their upstream regulators, the centralspindlin complex and Ect2, remained unaffected. These results suggested that mammalian IQGAP proteins may play a role in cytokinesis by regulating the localization of key cytokinesis regulatory proteins to the contractile apparatus during mitosis.

    DOI: 10.1111/gtc.12179

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  • Cell-Autonomous Enhancement of Glutamate-Uptake by Female Astrocytes 査読

    Yosuke Morizawa, Kaoru Sato, Junpei Takaki, Asami Kawasaki, Keisuke Shibata, Takeshi Suzuki, Shigeru Ohta, Schuichi Koizumi

    CELLULAR AND MOLECULAR NEUROBIOLOGY   32 ( 6 )   953 - 956   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER/PLENUM PUBLISHERS  

    Since gonadal female hormones act on and protect neurons, it is well known that the female brain is less vulnerable to stroke or other brain insults than the male brain. Although glial functions have been shown to affect the vulnerability of the brain, little is known if such a sex difference exists in glia, much less the mechanism that might cause gender-dependent differences in glial functions. In this study, we show that in vitro astrocytes obtained from either female or male pups show a gonadal hormone-independent phenotype that could explain the gender-dependent vulnerability of the brain. Female spinal astrocytes cleared more glutamate by GLAST than male ones. In addition, motoneurons seeded on female spinal astrocytes were less vulnerable to glutamate than those seeded on male ones. It is suggested that female astrocytes uptake more glutamate and reveal a stronger neuroprotective effect against glutamate than male ones. It should be noted that such an effect was independent of gonadal female hormones, suggesting that astrocytes have cell-autonomous regulatory mechanisms by which they transform themselves into appropriate phenotypes.

    DOI: 10.1007/s10571-012-9829-z

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  • MODULATION OF CONNEXIN 43 IN ROTENONE-INDUCED MODEL OF PARKINSON'S DISEASE 査読

    A. Kawasaki, T. Hayashi, K. Nakachi, J. E. Trosko, K. Sugihara, Y. Kotake, S. Ohta

    NEUROSCIENCE   160 ( 1 )   61 - 68   2009年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Gap junctional communication plays an important role in various models of brain pathology, but the changes of gap junctions in Parkinsonism are still not understood. In this study, we show that a major gap junctional protein, connexin43 (Cx43), in astrocytes is enhanced both in a rat Parkinson's disease (PD) model induced with rotenone, a widely used pesticide that inhibits mitochondrial complex 1, and in vitro in cultured astrocytes stimulated with rotenone. Enhancement of Cx43 protein levels in rotenone-treated cultured astrocytes occurred in parallel with an increase in gap junctional intercellular communication, but was not accompanied with an increase in Cx43 mRNA levels. Furthermore, the rotenone-induced increase of Cx43 protein levels both in vitro and in vivo was associated with increased levels of phosphorylated Cx43, which is required for gap junctional intercellular communication. In our rat PD model, phosphorylated C)43 was selectively enhanced in the basal ganglia regions, which contain DA neurons or their terminal areas. The increase of Cx43 levels was lower in the substantia nigra pars compacta and the striatum than in the substantia nigra pars reticulata and the globus pallidus. Our findings indicate that modulation of Cx43 protein, and consequently gap junctional cellular communication, in astrocytes may play an important role in PD pathology. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.neuroscience.2009.01.080

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産業財産権

  • 抗GAP43抗体

    五十嵐道弘, 武内恒成, 河嵜麻実, 野住素広

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    出願番号:特願PCT/JP2012/077163  出願日:2012年10月

    公開日:2013年4月

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共同研究・競争的資金等の研究

  • リン酸化GAP-43を指標とする神経再生促進薬の探索

    2014年9月 - 2015年4月

    独立行政法人 医薬基盤研究所  創薬支援ネットワーク 

    河嵜 麻実

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    担当区分:研究代表者  資金種別:競争的資金

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  • 神経回路形成における新規リン酸化GAP43の機能解析

    研究課題/領域番号:25870251  2013年 - 2014年

    文部科学省  科学研究費補助金(若手研究(B))  若手研究(B)

    河嵜 麻実

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    担当区分:研究代表者  資金種別:競争的資金

    配分額:4290000円 ( 直接経費:3300000円 、 間接経費:990000円 )

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