Faculty of Medicine School of Medicine Lecturer
Graduate School of Medical and Dental Sciences Molecular and Cellular Medicine Lecturer
Updated on 2025/07/02
博士(情報工学) ( 2003.3 九州工業大学 )
Endocytosis
Actin
Axon guidance
包括脳ネットワーク
Cytoskeleton
Organelles
3D-STED
3D-SIM
Super-resolution microscopy
Membrane trafficking
Cell motility
Growth cone
Life Science / Cell biology
Life Science / Neuroscience-general
Universite de Bordeaux Institute for Interdisciplinary Neuroscience Visiting researcher
2021.1 - 2021.6
Country:France
Universite de Bordeaux Institute for Interdisciplinary Neuroscience Visiting researcher
2019.7 - 2019.12
Country:France
Niigata University Department of Neurochemistry Lecturer
2011.6
Niigata University Department of Neurochemistry Assistant Professor
2008.4 - 2011.5
Niigata University Department of Neurochemistry PostDoc Position
2005.4 - 2008.3
National Institute of Genetics Division of Brain Function PostDoc Position
2003.4 - 2005.3
Niigata University Faculty of Medicine School of Medicine Lecturer
2011.6
Niigata University Graduate School of Medical and Dental Sciences Molecular and Cellular Medicine Lecturer
2011.6
Niigata University Graduate School of Medical and Dental Sciences Molecular and Cellular Medicine Assistant Professor
2007.4 - 2011.5
九州工業大学大学院情報工学研究科情報科学専攻
1998.4 - 2003.3
九州工業大学情報工学部生物科学システム工学科
1996.4 - 1998.3
THE JAPANESE BIOCHEMICAL SOCIETY
THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
THE JAPANESE SOCIETY FOR NEUROCHEMISTRY
THE JAPAN NEUROSCIENCE SOCIETY
Visualization of nanostructures in neuronal growth cones and axons using super-resolution structured illumination microscopy Invited Reviewed
Motohiro Nozumi, Michihiro Igarashi
Anatomical Science International 2025.6
Ladinin-1 in actin arcs of oral squamous cell carcinoma is involved in cell migration and epithelial phenotype. Reviewed International journal
Tatsuya Abé, Manabu Yamazaki, Motohiro Nozumi, Satoshi Maruyama, Kaori Takamura, Riuko Ohashi, Yoichi Ajioka, Jun-Ichi Tanuma
Scientific reports 14 ( 1 ) 22778 - 22778 2024.10
Identification of <i>z</i>‐axis filopodia in growth cones using super‐resolution microscopy Reviewed
Motohiro Nozumi, Yuta Sato, Miyako Nishiyama‐Usuda, Michihiro Igarashi
Journal of Neurochemistry 2024.7
Very-long-chain fatty acids are crucial to neuronal polarity by providing sphingolipids to lipid rafts. Reviewed International journal
Atsuko Honda, Motohiro Nozumi, Yasuyuki Ito, Rie Natsume, Asami Kawasaki, Fubito Nakatsu, Manabu Abe, Haruki Uchino, Natsuki Matsushita, Kazutaka Ikeda, Makoto Arita, Kenji Sakimura, Michihiro Igarashi
Cell reports 113195 - 113195 2023.10
JNK1-Dependent Phosphorylation of GAP-43 Serine 142 is a Novel Molecular Marker for Axonal Growth. Reviewed International journal
Masayasu Okada, Yosuke Kawagoe, Toshiyuki Takasugi, Motohiro Nozumi, Yasuyuki Ito, Hayato Fukusumi, Yonehiro Kanemura, Yukihiko Fujii, Michihiro Igarashi
Neurochemical research 47 ( 9 ) 2668 - 2682 2022.9
Correction to: JNK1‑Dependent Phosphorylation of GAP‑43 Serine 142 is a Novel Molecular Marker for Axonal Growth. Reviewed International journal
Masayasu Okada, Yosuke Kawagoe, Toshiyuki Takasugi, Motohiro Nozumi, Yasuyuki Ito, Hayato Fukusumi, Yonehiro Kanemura, Yukihiko Fujii, Michihiro Igarashi
Neurochemical research 47 ( 9 ) 2683 - 2683 2022.9
Phosphorylation of GAP-43 T172 is a molecular marker of growing axons in a wide range of mammals including primates. Reviewed International journal
Masayasu Okada, Yosuke Kawagoe, Yuta Sato, Motohiro Nozumi, Yuya Ishikawa, Atsushi Tamada, Hiroyuki Yamazaki, Yuko Sekino, Yonehiro Kanemura, Yohei Shinmyo, Hiroshi Kawasaki, Naoko Kaneko, Kazunobu Sawamoto, Yukihiko Fujii, Michihiro Igarashi
Molecular brain 14 ( 1 ) 66 - 66 2021.4
Coactosin Promotes F-Actin Protrusion in Growth Cones Under Cofilin-Related Signaling Pathway. Reviewed International journal
Xubin Hou, Motohiro Nozumi, Harukazu Nakamura, Michihiro Igarashi, Sayaka Sugiyama
Frontiers in cell and developmental biology 9 660349 - 660349 2021
Microtubule elongation along actin filaments induced by microtubule-associated protein 4 contributes to the formation of cellular protrusions. Reviewed International journal
Chihiro Doki, Kohei Nishida, Shoma Saito, Miyuki Shiga, Hikari Ogara, Ayumu Kuramoto, Masahiro Kuragano, Motohiro Nozumi, Michihiro Igarashi, Hiroyuki Nakagawa, Susumu Kotani, Kiyotaka Tokuraku
Journal of biochemistry 168 ( 3 ) 295 - 303 2020.9
Isoform-dependent subcellular localization of LMTK1A and LMTK1B and their roles in axon outgrowth and spine formation. Reviewed International journal
Ran Wei, Arika Sugiyama, Yuta Sato, Motohiro Nozumi, Hironori Nishino, Miyuki Takahashi, Taro Saito, Kanae Ando, Mitsunori Fukuda, Mineko Tomomura, Michihiro Igarashi, Shin-Ichi Hisanaga
Journal of biochemistry 168 ( 1 ) 23 - 32 2020.7
Neuronal Signaling Involved in Neuronal Polarization and Growth: Lipid Rafts and Phosphorylation. Reviewed International journal
Michihiro Igarashi, Atsuko Honda, Asami Kawasaki, Motohiro Nozumi
Frontiers in molecular neuroscience 13 150 - 150 2020
New observations in neuroscience using superresolution microscopy. Reviewed International journal
Michihiro Igarashi, Motohiro Nozumi, Ling-Gang Wu, Francesca Cella Zanacchi, István Katona, László Barna, Pingyong Xu, Mingshu Zhang, Fudong Xue, Edward Boyden
The Journal of neuroscience : the official journal of the Society for Neuroscience 38 ( 44 ) 9459 - 9467 2018.10
Vesicular movements in the growth cone. Reviewed International journal
Motohiro Nozumi, Michihiro Igarashi
Neurochemistry international 119 71 - 76 2018.10
Growth Cone Phosphoproteomics Reveals that GAP-43 Phosphorylated by JNK Is a Marker of Axon Growth and Regeneration. Reviewed International journal
Asami Kawasaki, Masayasu Okada, Atsushi Tamada, Shujiro Okuda, Motohiro Nozumi, Yasuyuki Ito, Daiki Kobayashi, Tokiwa Yamasaki, Ryo Yokoyama, Takeshi Shibata, Hiroshi Nishina, Yutaka Yoshida, Yukihiko Fujii, Kosei Takeuchi, Michihiro Igarashi
iScience 4 190 - 203 2018.6
Extracellular Signals Induce Glycoprotein M6a Clustering of Lipid Rafts and Associated Signaling Molecules. Reviewed International journal
Atsuko Honda, Yasuyuki Ito, Kazuko Takahashi-Niki, Natsuki Matsushita, Motohiro Nozumi, Hidenori Tabata, Kosei Takeuchi, Michihiro Igarashi
The Journal of neuroscience : the official journal of the Society for Neuroscience 37 ( 15 ) 4046 - 4064 2017.4
Coordinated Movement of Vesicles and Actin Bundles during Nerve Growth Revealed by Superresolution Microscopy. Reviewed International journal
Motohiro Nozumi, Fubito Nakatsu, Kaoru Katoh, Michihiro Igarashi
Cell reports 18 ( 9 ) 2203 - 2216 2017.2
Expression and function of neuronal growth-associated proteins (nGAPs) in PC12 cells. Reviewed International journal
Jia Lu, Motohiro Nozumi, Kosei Takeuchi, Haruki Abe, Michihiro Igarashi
Neuroscience research 70 ( 1 ) 85 - 90 2011.5
A stochastic model of neuronal growth cone guidance regulated by multiple sensors. Reviewed International journal
Taichiro Kobayashi, Kenshi Terajima, Motohiro Nozumi, Michihiro Igarashi, Kouhei Akazawa
Journal of theoretical biology 266 ( 4 ) 712 - 22 2010.10
Identification of functional marker proteins in the mammalian growth cone. Reviewed International journal
Motohiro Nozumi, Tetsuya Togano, Kazuko Takahashi-Niki, Jia Lu, Atsuko Honda, Masato Taoka, Takashi Shinkawa, Hisashi Koga, Kosei Takeuchi, Toshiaki Isobe, Michihiro Igarashi
Proceedings of the National Academy of Sciences of the United States of America 106 ( 40 ) 17211 - 6 2009.10
A novel method for RNA interference in neurons using enhanced green fluorescent protein (EGFP)-transgenic rats. Reviewed International journal
Jia Lu, Motohiro Nozumi, Hiroshi Fujii, Michihiro Igarashi
Neuroscience research 61 ( 2 ) 219 - 24 2008.6
IRSp53 is colocalised with WAVE2 at the tips of protruding lamellipodia and filopodia independently of Mena. Reviewed International journal
Hiroyuki Nakagawa, Hiroaki Miki, Motohiro Nozumi, Tadaomi Takenawa, Shigeaki Miyamoto, Jürgen Wehland, J Victor Small
Journal of cell science 116 ( Pt 12 ) 2577 - 83 2003.6
Differential localization of WAVE isoforms in filopodia and lamellipodia of the neuronal growth cone. Reviewed International journal
Motohiro Nozumi, Hiroyuki Nakagawa, Hiroaki Miki, Tadaomi Takenawa, Shigeaki Miyamoto
Journal of cell science 116 ( Pt 2 ) 239 - 46 2003.1
リン酸化プロテオミクスで同定された、神経成長関連分子群の責任キナーゼの解析
河嵜 麻実, 小林 大記, 岡田 正康, 野住 素広, 武内 恒成, 五十嵐 道弘
日本生化学会大会・日本分子生物学会年会合同大会講演要旨集 88回・38回 [4T17L - 12(3P0403)] 2015.12
Membrane retrieval coincides with the filopodia formation in the neuronal growth cone.
M. Nozumi, M. Igarashi
MOLECULAR BIOLOGY OF THE CELL 25 2014.12
The in vivo GAP-43 phosphorylation sites involved in axon growth or regeneration
A. Kawasaki, M. Nozumi, K. Takeuchi, N. Yoshioka, M. Igarashi
JOURNAL OF NEUROCHEMISTRY 123 56 - 56 2012.10
Functions of newly identified nerve growth-associated proteins in the growth cone
Motohiro Nozumi, Jia Lu, Kosei Takeuchi, Michihiro Igarashi
NEUROSCIENCE RESEARCH 65 S97 - S97 2009
RNAi-mediated knockdown of FABP-7 inhibits neurite outgrowth in developing cortical neurons
Jia Lu, Motohiro Nozumi, Haruki Abe, Michihiro Igarashi
NEUROSCIENCE RESEARCH 61 S233 - S233 2008
Dynamics of actin-associated proteins detected by proteomics of growth cone
Satoe Ebihara, Shirakawa Sayurni, Todaka Reiko, Nozumi Motohiro, Ozawa Mutsumi, Igarshi Michihiro, Katoh Kaoru
NEUROSCIENCE RESEARCH 61 S89 - S89 2008
2P221 Actin-associate proteins indentified by proteomics of growth cones showed ten types of dynamic behaviors(Cell biological problems-adhesion, motility, cytoskeleton, signaling, and membrane,Oral Presentations)
Ebihara Satoe, Shirakawa Sayumi, Todaka Reiko, Nozumi Motohiro, Ozawa Mutsumi, Igarashi Michihiro, Katoh Karoru
Seibutsu Butsuri 47 ( 1 ) S168 2007.11
Systematic immunostaining and RNAi experiments for finding the novel molecular marker candidates of the growth cone
Motohiro Nozumi, Kazuko Takahashi-Niki, Michihiro Igarashi
NEUROSCIENCE RESEARCH 58 S201 - S201 2007
Imaging of f-actin and actin associate proteins in neuronal growth cones Reviewed
Katoh Kaoru, Shirakawa Sayumi, Todaka Reiko, Miyoshi Hiromi, Ozawa Mutsumi, Nozumi Motohiro, Igarashi Michihiro
Journal of Pharmacological Sciences 103 47P 2007
成長円錐のプロテオミクスによるアクチン調節タンパク質の同定
野住素広, 加藤薫, 五十嵐道弘
バイオイメージング 15 ( 2 ) 45 - 46 2006.10
プロテオミクスの手法で同定された成長円錐のアクチン関連蛋白質の網羅的動態観察
白川彩弓, 戸高玲子, 野住素広, 小澤睦, 五十嵐道弘, 加藤薫
バイオイメージング 15 ( 2 ) 85 - 86 2006.10
Proteomics of the growth cone: II. The systematic immunostaining analysis of the growth cone proteins identified by the proteomic research
Motohiro Nozumi, Michihiro Igarashi
NEUROSCIENCE RESEARCH 55 S86 - S86 2006
WAVE/scar proteins control filopodial formation on neuronal growth cones
M Nozumi, H Nakagawa, H Miki, T Takenawa, S Miyamoto
MOLECULAR BIOLOGY OF THE CELL 13 58A - 58A 2002.11
Localization of GAP-43 in KCl depolarized PC12 cells
Nozumi M., Nakagawa H., Miyamoto S.
Biophysics 38 ( 2 ) S199 1998.9
Visualization of various organelle dynamics by super-resolution shadow imaging of intracellular space.
Nozumi M, Igarashi M, Nägerl UV
Neuro2022 2022.7
Actin reorganization and plasma membrane trafficking in three-dimensional space of nerve growth cones. Invited
Nozumi M, Igarashi M
2021.3
Super-resolution shadow imaging of intracellular space reveals various organelle dynamics in living cells. Invited
Nozumi M, Igarashi M
2020.9
構造化照明による超解像顕微鏡(SIM)で明らかになった神経突起先端の3次元構造 Invited
野住素広, 五十嵐道弘
第 41 回日本分子生物学会年会 ワークショップ 2018.11
New relationships between F actin organization and membrane trafficking in the growth cone revealed by SIM. Invited
Nozumi M, Igarashi M
2018.10
神経成長におけるエンドサイトーシスとアクチン再編の関係 Invited
野住素広, 五十嵐道弘
2017年度生命科学系学会合同年次大会(ConBio2017)ワークショップ「エンドサイトーシス生物学の新展開」 2017.12
構造化照明顕微鏡(SIM)観察で明らかになった神経成長時のアクチン再編とエンドサイトーシスの新しい関係 Invited
野住素広, 五十嵐道弘
第 89 回日本生化学会大会 フォーラム「超解像度顕微鏡による生化学の近未来的視点」 2016.9
成長円錐のプロテオミクスによるアクチン調節タンパク質の同定 Invited
野住素広, 加藤薫, 五十嵐道弘
日本バイオイメージング学会第15回学術集会 2006.11
GAP43の免疫学的分析方法及び当該方法を実行するためのキット、並びに成長円錐を検出するための試薬及び方法
武内 恒成, 五十嵐 道弘, 野住 素広, 河嵜 麻実
抗GAP43抗体
武内 恒成, 五十嵐 道弘, 野住 素広, 河嵜 麻実
The Role and Diversity of Cortical Interneurons in Modulating Brain Function
Grant number:24K09642
2024.4 - 2027.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )
Mechanism of tunneling nanotubes formation as a key step in brain tumor progression
Grant number:24K12217
2024.4 - 2027.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )
脳腫瘍の悪性化に関係するナノチューブネットワーク形成機構の解明
2023.4 - 2024.3
System name:高橋産業経済研究財団
Development of a novel method for live cell imaging of intracellular and extracellular structures in brain tissue
Grant number:21K19305
2021.7 - 2023.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Challenging Research (Exploratory)
Awarding organization:Japan Society for the Promotion of Science
Nozumi Motohiro
Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )
Super-resolution shadow imaging (SUSHI) enables the acquisition of shadow images of brain tissue, including synapses, through fluorescent labeling of the extracellular space. In this study, we applied the principles of SUSHI to visualize intracellular structures, aiming to simultaneously image both intracellular and extracellular structures in living brain tissue. By employing this approach, the shadow imaging of growth cones, which are axonal tips, revealed a novel relationship between mitochondrial morphology and the actin cytoskeleton during nerve growth processes. To achieve this, a red fluorescent protein was introduced into fetal mouse brains through in utero electroporation. Through this method, we successfully captured intracellular structures of the neuron in the shadow images generated from red fluorescence signals. We also observed a growth cone extending its filopodium towards a neighboring cell, followed by cell-cell contact using our imaging technique.
Mechanisms of Neural Axon Growth Regulation via Very Long-Chain Fatty Acid Synthesis
Grant number:21K06390
2021.4 - 2024.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
HONDA ATSUKO
Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )
We generated knock out mice lacking the enzyme for the fatty acid elongase of VLCFAs, GPSN2 abundant in the neuronal growth cones. Homozygous knockout mice exhibited embryonic lethality and the suppression of neural circuit formation. Lipidomic analysis revealed decreased production of gangliosides, particularly a components of lipid rafts containing very long-chain fatty acid ceramide metabolites, due to GPSN2 deficiency. In neurons with heterozygous knockout or suppressed expression of GPSN2, lipid raft formation and neuronal polarity were inhibited. These findings suggests that very long-chain fatty acids induce lipid raft formation via ganglioside production, crucially regelatlling neuronal polarity determination and axon growth. (A Honda et al, Cell Reports 2023)
神経組織における細胞内構造の網羅的可視化技術の開発
2021.4 - 2022.3
System name:技術開発研究助成【開発研究】
Awarding organization:中谷医工計測技術振興財団
網羅的オルガネラ動態の可視化による神経成長機構の解明
2020.4 - 2021.3
System name:基礎科学研究助成
Awarding organization:住友財団
Nano-imaging of intracellular structures and membrane trafficking in the extending neuronal growth cone
2019.7 - 2020.2
Awarding organization:Université de Bordeaux
Actin-dependent endocytosis producing the driving force for neuronal growth
Grant number:18K06459
2018.4 - 2021.3
System name:Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Motohiro Nozumi
Authorship:Principal investigator Grant type:Competitive
Growth cones are essential for proper neural circuit formation and axon regeneration. At the leading edge of the growth cone, the plasma membrane is pushed by the elongation of the actin cytoskeleton, which generates the driving force for axon outgrowth. In this study, we found non-adhesive filopodia extending vertically from the surface of the growth cones by three-dimensional observations using super-resolution microscopy, 3D-SIM. The filopodia showed lipid raft-dependent accumulation of neuropilin-1, an axon guidance receptor, and repeated elongation and retraction within a minute. The growth cone could actively recruit receptors toward the extracellular space through the filopodia elongation, suggesting that they efficiently capture soluble ligands.
神経成長・再生を誘導する成長円錐のアクチン依存性エンドサイトーシス
2017.4 - 2019.3
System name:医学系研究助成
Awarding organization:武田科学振興財団
Nano-imaging of the growth cone producing the driving force for neuronal growth
Grant number:17KK0144
2017 - 2021
System name:Fund for the Promotion of Joint International Research (Fostering Joint International Research)
Research category:Fund for the Promotion of Joint International Research (Fostering Joint International Research)
Awarding organization:Japan Society for the Promotion of Science
Motohiro Nozumi
Authorship:Principal investigator Grant type:Competitive
To observe the interaction between various organelles and the cytoskeleton in the growth cones that lead neurites, we applied super-resolution shadow imaging, SUSHI, to visualize the intracellular structure. Using cultured cells that cytosolically expressed the fluorescent protein Citrine, we could readily discriminate negative images of various organelles, such as mitochondria, endoplasmic reticulum, endosome, lysosome and also cytoskeleton. The live shadow imaging showed that vesicles arising following actin polymerization and organelles that repeatedly move to the leading edge against the actin retrograde flow. Mitochondrial fission was also detected immediately after a vesicle approached the mitochondrion in the growth cone. The super-resolution imaging is one of the best methods to visualize multiple organelles and cytoskeletons simultaneously.
Coordinated vesicular transport and actin reorganization in the neuronal growth cone
Grant number:15K06769
2015.4 - 2018.3
System name:Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Motohiro Nozumi
Authorship:Principal investigator Grant type:Competitive
The neuronal growth cone is a motile tip of the growing axon at the times of the neuronal development/regeneration. The continuous reorganization of actin cytoskeleton and the membrane retrieval from the plasma membrane are observed in the leading edge of the growth cone. We found that the synaptophysin (Syp)-positive vesicles arose near the filopodia, and most of them were retrogradely moving along the actin bundles, using a superresolution microscopy 3D-SIM. The retrogradely moving Syp were colocalized with a BAR domain protein, endophilin A3 (Endo3), and dynamin 1 (Dnm1), but not with clathrin. Whereas clathrin mainly accumulated in the basal membrane in the central domain of the growth cone, Endo3 emerged in the dorsal surface. Accumulation of Endo3 depended on the F-actin bundling. These results suggest that the Syp-vesicles by Endo3-, Dnm1-dependent and clathrin-independent endocytosis, occurring at the apical membrane of the leading edge, coincides with filopodial formation.
Membrane dynamics and fatty acid elongation in the growth cone
Grant number:24700318
2012.4 - 2015.3
System name:Grant-in-Aid for Young Scientists (B)
Research category:Grant-in-Aid for Young Scientists (B)
Awarding organization:Japan Society for the Promotion of Science
Motohiro Nozumi
Authorship:Principal investigator Grant type:Competitive
The growth cone is a highly motile structure in the growing tip of a neurite at the stages of the neuronal development and regeneration. To visualize the vesicular trafficking and actin reorganization of the growth cone in detail simultaneously, we analyzed the dynamics of GFP-synaptophysin and mCherry-actin in the growth cone of NG108-15 cells, using a superresolution microscopy. We found that the vesicles arose near the root portions of filopodia, and most of them were retrogradely moving along the actin bundles. Both of such events were inhibited by actin polymerization inhibitors or a myosin light-chain kinase inhibitor. RNAi of fascin also decreased the numbers of vesicles localized at the leading edge. Moreover, the retrogradely moving vesicles were not colocalized with clathrin but with endophilin and dynamin. These results suggest that there is a novel mechanism of membrane retrieval in the growth cone.
The role of neuronal growth-associated proteins in the growth cone
Grant number:22700329
2010.4 - 2012.3
System name:Grant-in-Aid for Young Scientists (B)
Research category:Grant-in-Aid for Young Scientists (B)
Awarding organization:Japan Society for the Promotion of Science
Motohiro Nozumi
Authorship:Principal investigator Grant type:Competitive
Continuous rearrangement of cytoskeletons and recruitment of transport vesicles to plasma membrane are thought to be essential to the growth cone motility, however, it is unclear how the proteins are directly involved in processes of axonal growth. We have recently reported of identifying 17 proteins as the functional marker proteins in the mammalian growth cone, as neuronal growth-associated proteins(nGAPs). However, it remains to be determined whether these marker proteins are widely involved in neuritogenesis in various neuronal cell types. We showed that all of the nGAPs were concentrated in the growth cone of PC12 cell. RNAi knockdown of each nGAPs also inhibited neurite elongation induced by NGF. These results indicate that nGAPs are involved in NGF-induced neurite outgrowth, and that nGAPs are very useful as generalized marker of the growth cone. To investigate the roles of nGAPs for the growth cone behavior further, we analyzed the changes of morphology of the growth cone and cytoskeletal distribution there using RNAi. We found that knockdown of some nGAPs caused significant reduction of the growth cone area and the amount of F-actin there. These results suggest that these nGAPs regulate growth cone morphology through rearrangement of F-actin and thereby control the axon outgrowth.
A study of searching the growth cone proteins involved in synapse formation
Grant number:17023019
2005.4 - 2010.3
System name:Grant-in-Aid for Scientific Research on Priority Areas
Research category:Grant-in-Aid for Scientific Research on Priority Areas
Awarding organization:Japan Society for the Promotion of Science
Michihiro Igarashi
Grant type:Competitive
The growth cone is the essential structure for accurate synaptogenesis to form the brain neuronal network, however, in the mammalian brain, the molecular basis is poorly understood. Here, we successfully used a proteomic approach to identify 945 proteins, including highly abundant, membrane-associated and actin-associated proteins. Almost one hundred of the proteins appear to be highly enriched in the growth cone, as determined by quantitative immunostaining, and for 18 proteins, the results of RNAi suggest a role in axon growth. Most of the proteins we identified have not previously been implicated in axon growth and thus, their identification presents a significant first step forward, providing new marker proteins and candidate neuronal growth-associated proteins.
分子生物学
生体内物質と代謝
出前講義「マイクロマシンとしての細胞」(福島県立白河高校)
Role(s): Lecturer
2017.9