Updated on 2024/03/29

写真a

 
ROSALES ROCABADO JUAN MARCELO
 
Organization
Academic Assembly Institute of Medicine and Dentistry SHIGAKU KEIRETU Assistant Professor
Graduate School of Medical and Dental Sciences Oral Life Science Oral Health Science Assistant Professor
Title
Assistant Professor
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Degree

  • Doctor of Dental Surgery ( 2002.12   Nuestra Señora de La Paz University )

  • Community Oral Health Care ( 2006.5 )

  • Ph.D. ( 2012.3   Niigata University )

Research History

  • Niigata University   Graduate School of Medical and Dental Sciences Oral Life Science Oral Health Science   Assistant Professor

    2013.9

Education

  • Faculty of Dentistry & Graduate School of Medical and Dental sciences   Division of Bio-Prosthodontics

    2008.4 - 2012.3

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    Country: Japan

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Papers

  • 本学歯学部学生の歯冠修復学実習における支台歯形成自己評価能力について

    長澤 麻沙子, 秋葉 奈美, 秋葉 陽介, 加来 賢, 青柳 裕仁, Rosales Rocabado J.M., 江口 香里, 魚島 勝美

    日本歯科医学教育学会総会・学術大会プログラム・抄録集   38回   94 - 94   2019.6

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    Language:Japanese   Publisher:(一社)日本歯科医学教育学会  

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  • A multi-factorial analysis of bone morphology and fracture strength of rat femur in response to ovariectomy

    Juan Marcelo Rosales Rocabado, Masaru Kaku, Kosuke Nozaki, Takako Ida, Megumi Kitami, Yujin Aoyagi, Katsumi Uoshima

    JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH   13   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BMC  

    BackgroundPostmenopausal osteoporosis develops due to a deficiency of estrogen that causes a decrease in bone mass and changes in the macro- and micro-architectural structure of the bone, leading to the loss of mechanical strength and an increased risk of fracture. Although the assessment of bone mineral density (BMD) has been widely used as a gold standard for diagnostic screening of bone fracture risks, it accounts for only a part of the variation in bone fragility; thus, it is necessary to consider other determinants of bone strength. Therefore, we aimed to comprehensively evaluate the architectural changes of the bone that influence bone fracture strength, together with the different sensitivities of cortical and trabecular bone in response to ovariectomy (OVX).MethodsBone morphology parameters were separately analyzed both in cortical and in trabecular bones, at distal-metaphysis, and mid-diaphysis of OVX rat femurs. Three-point bending test was performed at mid-diaphysis of the femurs. Correlation of OVX-induced changes of morphological parameters with breaking force was analyzed using Pearson's correlation coefficient.ResultsOVX resulted in a decline in the bone volume of distal-metaphysis trabecular bone, but an increase in distal-metaphysis and mid-diaphysis cortical bone volume. Tissue mineral density (TMD) remained unchanged in both the trabecular and cortical bone of the distal metaphysis but decreased in cortical bone of the mid-diaphysis. The OVX significantly increased the breaking force at mid-diaphysis of the femurs.ConclusionsOVX decreased the trabecular bone volume of the distal-metaphysis and increased the cortical bone volume of the distal-metaphysis and mid-diaphysis. Despite the reduction in TMD and increased cortical porosity, bone fracture strength increased in the mid-diaphysis after OVX. These results indicate that analyzing a single factor, i.e., BMD, is not sufficient to predict the absolute fracture risk of the bone, as OVX-induced bone response vary, depending on the bone type and location. Our results strongly support the necessity of analyzing bone micro-architecture and site specificity to clarify the true etiology of osteoporosis in a clinical setting.

    DOI: 10.1186/s13018-018-1018-4

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  • Extracellular matrix with defective collagen cross-linking affects the differentiation of bone cells. Reviewed International journal

    Takako Ida, Masaru Kaku, Megumi Kitami, Masahiko Terajima, Juan Marcelo Rosales Rocabado, Yosuke Akiba, Masako Nagasawa, Mitsuo Yamauchi, Katsumi Uoshima

    PloS one   13 ( 9 )   e0204306   2018

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    Fibrillar type I collagen, the predominant organic component in bone, is stabilized by lysyl oxidase (LOX)-initiated covalent intermolecular cross-linking, an important determinant of bone quality. However, the impact of collagen cross-linking on the activity of bone cells and subsequent tissue remodeling is not well understood. In this study, we investigated the effect of collagen cross-linking on bone cellular activities employing a loss-of-function approach, using a potent LOX inhibitor, β-aminopropionitrile (BAPN). Osteoblastic cells (MC3T3-E1) were cultured for 2 weeks in the presence of 0-2 mM BAPN to obtain low cross-linked collagen matrices. The addition of BAPN to the cultures diminished collagen cross-links in a dose-dependent manner and, at 1 mM level, none of the major cross-links were detected without affecting collagen production. After the removal of cellular components from these cultures, MC3T3-E1, osteoclasts (RAW264.7), or mouse primary bone marrow-derived stromal cells (BMSCs) were seeded. MC3T3-E1 cells grown on low cross-link matrices showed increased alkaline phosphatase (ALP) activity. The number of multinucleate tartrate-resistant acid phosphatase (TRAP)-positive cells increased in RAW264.7 cells. Initial adhesion, proliferation, and ALP activity of BMSCs also increased. In the animal experiments, 4-week-old C57BL/6 mice were fed with BAPN-containing diet for 8 weeks. At this point, biochemical analysis of bone demonstrated that collagen cross-links decreased without affecting collagen content. Then, the diet was changed to a control diet to minimize the direct effect of BAPN. At 2 and 4 weeks after the change, histological samples were prepared. Histological examination of femur samples at 4 weeks showed a significant increase in the number of bone surface osteoblasts, while the bone volume and surface osteoclast numbers were not significantly affected. These results clearly demonstrated that the extent of collagen cross-linking of bone matrix affected the differentiation of bone cells, underscoring the importance of collagen cross-linking in the regulation of cell behaviors and tissue remodeling in bone. Characterization of collagen cross-linking in bone may be beneficial to obtain insight into not only bone mechanical property, but also bone cellular activities.

    DOI: 10.1371/journal.pone.0204306

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  • Recruitment of bone marrow-derived cells to the periodontal ligament via the stromal cell-derived factor-1/C-X-C chemokine receptor type 4 axis Reviewed

    M. Kaku, M. Kitami, J. M. Rosales Rocabado, T. Ida, Y. Akiba, K. Uoshima

    Journal of Periodontal Research   52 ( 4 )   686 - 694   2017.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Background/Objectives: The periodontal ligament (PDL) is a non-mineralized connective tissue that exists between the alveolar bone and root surface cementum and plays important roles in tooth function. The PDL harbors a remarkable reserve of multipotent stem cells, which maintain various types of cells. However, the sources of these stem cells, other than their developmental origin, are not well understood. Material and Methods: To elucidate the recruitment of bone marrow (BM)-derived stem cells in the PDL, green fluorescent protein (GFP)-expressing BM-derived cells were transplanted into the femoral BM of immunodeficient rats, and the distribution and expression of stem cell markers in the PDL were analyzed in vivo. To evaluate the functional significance of BM-derived cells to the PDL, tooth replantation was performed and the expression of stromal cell-derived factor (SDF)-1, a critical chemotactic signal for mesenchymal stem cell recruitment, was analyzed. To confirm the SDF-1-dependency of BM-derived cell migration to the PDL, PDL-conditioned medium (CM) was prepared, and BM-derived cell migration was analyzed using a transwell culture system. Results: Four weeks after cell transplantation, GFP-positive cells were detected in the PDL, and some of them were also positive for stem cell markers (i.e., CD29, SSEA4, and αSMA). Seven days after tooth replantation, the number of GFP- and SDF-1-positive cells significantly increased in PDL. Concurrently, the concentration of SDF-1 and the number of colony-forming units of fibroblasts in peripheral blood were increased. BM-derived cell migration increased in PDL-CM and was inhibited by an inhibitor of C-X-C chemokine receptor type 4 (CXCR4), an SDF-1 receptor. Conclusion: These results indicate that stem cells and their progeny in PDL are not only derived from their developmental origin but are also supplied from the BM via the blood as the need arises. Moreover, this BM-derived cell recruitment appears to be regulated, at least partially, by the SDF-1/CXCR4 axis.

    DOI: 10.1111/jre.12433

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  • Prolonged Survival of Transplanted Osteoblastic Cells Does Not Directly Accelerate the Healing of Calvarial Bone Defects International journal

    Megumi Kitami, Masaru Kaku, Juan Marcelo Rosales Rocabado, Takako Ida, Nami Akiba, Katsumi Uoshima

    Journal of Cellular Physiology   231 ( 9 )   1974 - 1982   2016.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    Considering the increased interest in cell-based bone regeneration, it is necessary to reveal the fate of transplanted cells and their substantive roles in bone regeneration. The aim of this study was to analyze the fate of transplanted cells and the effect of osteogenic cell transplantation on calvarial bone defect healing. An anti-apoptotic protein, heat shock protein (HSP) 27, was overexpressed in osteoblasts. Then, the treated osteoblasts were transplanted to calvarial bone defect and their fate was analyzed to evaluate the significance of transplanted cell survival. Transient overexpression of Hsp27 rescued MC3T3-E1 osteoblastic cells from H2 O2 -induced apoptosis without affecting osteoblastic differentiation in culture. Transplantation of Hsp27-overexpressing cells, encapsulated in collagen gel, showed higher proliferative activity, and fewer apoptotic cells in comparison with control cells. After 4-week of transplantation, both control cell- and Hsp27 overexpressed cell-transplanted groups showed significantly higher new bone formation in comparison with cell-free gel-transplantation group. Interestingly, the prolonged survival of transplanted osteoblastic cells by Hsp27 did not provide additional effect on bone healing. The transplanted cells in collagen gel survived for up to 4-week but did not differentiate into bone-forming osteoblasts. In conclusion, cell-containing collagen gel accelerated calvarial bone defect healing in comparison with cell-free collagen gel. However, prolonged survival of transplanted cells by Hsp27 overexpression did not provide additional effect. These results strongly indicate that cell transplantation-based bone regeneration cannot be explained only by the increment of osteogenic cells. Further studies are needed to elucidate the practical roles of transplanted cells that will potentiate successful bone regeneration. J. Cell. Physiol. 231: 1974-1982, 2016. © 2016 Wiley Periodicals, Inc.

    DOI: 10.1002/jcp.25302

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  • Mechanical Loading Stimulates Expression of Collagen Cross-Linking Associated Enzymes in Periodontal Ligament

    Masaru Kaku, Juan Marcelo Rosales Rocabado, Megumi Kitami, Takako Ida, Yosuke Akiba, Mitsuo Yamauchi, Katsumi Uoshima

    Journal of Cellular Physiology   231 ( 4 )   926 - 933   2016.4

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    Type I collagen, a major extracellular component of the periodontal ligament (PDL), is post-translationally modified by a series of specific enzymes. Among the collagen-modifying enzymes, lysyl oxidase (LOX) is essential to initiate collagen cross-linking and lysyl hydroxylases (LHs) to regulate the cross-linking pathways that are important for tissue specific mechanical properties. The purpose of this study was to investigate the effects of mechanical loading on the expression of collagen-modifying enzymes and subsequent tissue changes in PDL. Primary human PDL cells were subjected to mechanical loading in a 3D collagen gel, and gene expression and collagen component were analyzed. Wistar rats were subjected to excessive occlusal loading with or without intra-peritoneal injection of a LOX inhibitor, beta-aminopropionitrile (BAPN). Upon mechanical loading, gene expression of LH2 and LOX was significantly elevated, while that of COL1A2 was not affected on hPDL-derived cells. The mechanical loading also elevated formation of collagen a-chain dimers in 3D culture. The numbers of LH2 and LOX positive cells in PDL were significantly increased in an excessive occlusal loading model. Notably, an increase of LH2-positive cells was observed only at the bone-side of PDL. Intensity of picrosirius red staining was increased by excessive occlusal loading, but significantly diminished by BAPN treatment. These results demonstrated that mechanical loading induced collagen maturation in PDL by up-regulating collagen-modifying enzymes and subsequent collagen cross-linking which are important for PDL tissue maintenance. (C) 2015 Wiley Periodicals, Inc.

    DOI: 10.1002/jcp.25184

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  • Correlation Between Stress Distributions and Biological Reactions in Bone Surrounding Implants That Support Cantilevers in Supraocclusal Contact in Rats

    Ryohei Takano, Masako Nagasawa, Megumi Kitami, Juan Marcelo Rosales Rocabado, Masaru Kaku, Roxana Stegaroiu, Katsumi Uoshima

    Implant Dentistry   25 ( 2 )   204 - 213   2016.4

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    Publishing type:Research paper (scientific journal)   Publisher:Ovid Technologies (Wolters Kluwer Health)  

    DOI: 10.1097/id.0000000000000369

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  • Osteoblastic Differentiation and Mineralization Ability of Periosteum-Derived Cells Compared With Bone Marrow and Calvaria-Derived Cells

    Juan Marcelo Rosales-Rocabado, Masaru Kaku, Megumi Kitami, Yosuke Akiba, Katsumi Uoshima

    Journal of Oral and Maxillofacial Surgery   72 ( 4 )   694.e1 - 694.e9   2014.4

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.joms.2013.12.001

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  • Royal jelly affects collagen crosslinking in bone of ovariectomized rats

    Masaru Kaku, Juan Marcelo Rosales Rocabado, Megumi Kitami, Takako Ida, Katsumi Uoshima

    Journal of Functional Foods   7   398 - 406   2014.3

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.jff.2014.01.019

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MISC

  • Response to Letter to the Editor: Concerns on modeling postmenopausal osteoporosis in young female rats

    Juan Marcelo Rosales Rocabado, Masaru Kaku, Kosuke Nozaki, Takako Ida, Megumi Kitami, Yujin Aoyagi, Katsumi Uoshima

    JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH   14 ( 1 )   2019.12

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    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Publisher:BMC  

    DOI: 10.1186/s13018-019-1485-2

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Teaching Experience

  • 早期臨床実習IB

    2021
    Institution name:新潟大学

  • 早期臨床実習ⅠB

    2019
    Institution name:新潟大学

  • 欠損補綴学Ⅱ

    2018
    Institution name:新潟大学

  • 歯の形態学

    2018
    Institution name:新潟大学

  • 歯冠修復学

    2018
    Institution name:新潟大学

  • 早期臨床実習Ⅰ

    2017
    Institution name:新潟大学

  • 国際歯科保健医療学入門

    2017
    Institution name:新潟大学

  • 歯の形態と機能

    2017
    Institution name:新潟大学

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