2022/11/29 更新

写真a

ワタナベ ユミ
渡邊 裕美
WATANABE Yumi
所属
教育研究院 医歯学系 医学系列 准教授
医歯学総合研究科 地域疾病制御医学専攻 地域予防医学 准教授
職名
准教授
外部リンク

学位

  • 博士(医学) ( 2000年1月   九州大学 )

研究キーワード

  • Biochemistry and Molecular biology

  • 予防医学

  • 認知症

  • 疫学

研究分野

  • ライフサイエンス / 衛生学、公衆衛生学分野:実験系を含む

  • ライフサイエンス / 医化学  / 神経化学

経歴(researchmap)

  • 新潟大学大学院医歯学総合研究科地域疾病制御医学専攻地域予防医学大講座社会・環境医学   (医学部環境予防医学分野)   准教授

    2019年4月 - 現在

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    国名:日本国

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  • 新潟大学大学院医歯学総合研究科地域疾病制御医学専攻地域予防医学大講座社会・環境医学   (医学部環境予防医学分野)   講師

    2017年5月 - 2019年3月

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    国名:日本国

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  • 新潟大学   大学院医歯学総合研究科地域疾病制御医学専攻地域予防医学大講座社会・環境医学 (医学部環境予防医学分野)   助教

    2014年4月 - 2017年4月

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  • 新潟大学   大学院医歯学総合研究科   日本学術振興会特別研究員

    2011年4月 - 2014年3月

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  • 新潟大学超域研究機構   (医学部第二生化学)   研究員

    2009年4月 - 2011年3月

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  • 新潟大学大学院医歯学総合研究科   シグナル伝達講座分子細胞機能学分野(医学部第二生化学)   研究員

    2005年4月 - 2009年3月

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  • 京都大学医学研究科   生体構造学講座機能微細形態学   助手

    2003年5月 - 2005年3月

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  • 米国テキサス州立大学MD Anderson Cancer Center   分子遺伝学部門   博士研究員

    2001年7月 - 2003年1月

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  • 米国テキサス州立大学MD Anderson Cancer Center   生化学分子生物学部門   博士研究員

    2000年8月 - 2001年7月

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  • 九州大学生体防御医学研究所   感染防御学部問   助手

    2000年2月 - 2001年8月

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▶ 全件表示

経歴

  • 新潟大学   医歯学総合研究科 地域疾病制御医学専攻 地域予防医学   准教授

    2019年4月 - 現在

  • 新潟大学   医歯学総合研究科 地域疾病制御医学専攻 地域予防医学   講師

    2017年5月 - 2019年3月

  • 新潟大学   医歯学総合研究科 地域疾病制御医学専攻 地域予防医学   助教

    2014年4月 - 2017年4月

  • 新潟大学   医歯学総合研究科   日本学術振興会特別研究員

    2011年4月 - 2014年3月

  • 新潟大学   特別研究員

    2009年4月 - 2011年3月

  • 新潟大学   医歯学総合研究科 分子細胞医学専攻   科研費研究員

    2005年4月 - 2009年3月

▶ 全件表示

学歴

  • 九州大学大学院   医学系研究科博士課程   分子医学系専攻

    1995年4月 - 2000年1月

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  • 九州大学   医学部   医学科

    1989年4月 - 1995年3月

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所属学協会

  • 日本衛生学会

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  • 日本認知症学会

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  • 日本プロテオーム学会

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  • 日本産業衛生学会

    2020年 - 現在

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  • 環境ホルモン学会 [正式名称;日本内分泌撹乱化学物質学会]

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論文

  • Alterations in Glycerolipid and Fatty Acid Metabolic Pathways in Alzheimer's Disease Identified by Urinary Metabolic Profiling: A Pilot Study 査読 国際誌

    Yumi Watanabe, Kensaku Kasuga, Takayoshi Tokutake, Kaori Kitamura, Takeshi Ikeuchi, Kazutoshi Nakamura

    Frontiers in Neurology   12   719159 - 719159   2021年10月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Media SA  

    An easily accessible and non-invasive biomarker for the early detection of Alzheimer's disease (AD) is needed. Evidence suggests that metabolic dysfunction underlies the pathophysiology of AD. While urine is a non-invasively collectable biofluid and a good source for metabolomics analysis, it is not yet widely used for this purpose. This small-scale pilot study aimed to examine whether the metabolic profile of urine from AD patients reflects the metabolic dysfunction reported to underlie AD pathology, and to identify metabolites that could distinguish AD patients from cognitively healthy controls. Spot urine of 18 AD patients (AD group) and 18 age- and sex-matched, cognitively normal controls (control group) were analyzed by mass spectrometry (MS). Capillary electrophoresis time-of-flight MS and liquid chromatography–Fourier transform MS were used to cover a larger range of molecules with ionic as well as lipid characteristics. A total of 304 ionic molecules and 81 lipid compounds of 12 lipid classes were identified. Of these, 26 molecules showed significantly different relative concentrations between the AD and control groups (Wilcoxon's rank-sum test). Moreover, orthogonal partial least-squares discriminant analysis revealed significant discrimination between the two groups. Pathway searches using the KEGG database, and pathway enrichment and topology analysis using Metaboanalyst software, suggested alterations in molecules relevant to pathways of glycerolipid and glycerophospholipid metabolism, thermogenesis, and caffeine metabolism in AD patients. Further studies of urinary metabolites will contribute to the early detection of AD and understanding of its pathogenesis.

    DOI: 10.3389/fneur.2021.719159

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  • Urinary Apolipoprotein C3 Is a Potential Biomarker for Alzheimer’s Disease 査読

    Yumi Watanabe, Yoshitoshi Hirao, Kensaku Kasuga, Takayoshi Tokutake, Kaori Kitamura, Shumpei Niida, Takeshi Ikeuchi, Kazutoshi Nakamura, Tadashi Yamamoto

    Dementia and Geriatric Cognitive Disorders Extra   10 ( 3 )   94 - 104   2020年9月

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    担当区分:筆頭著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:S. Karger AG  

    Introduction: Biomarkers of Alzheimer’s disease (AD) that can easily be measured in routine health checkups are desirable. Urine is a source of biomarkers that can be collected easily and noninvasively. We previously reported on the comprehensive profile of the urinary proteome of AD patients and identified proteins estimated to be significantly increased or decreased in AD patients by a label-free quantification method. The present study aimed to validate urinary levels of proteins that significantly differed between AD and control samples from our proteomics study (i.e., apolipoprotein C3 [ApoC3], insulin-like growth factor-binding protein 3 [Igfbp3], and apolipoprotein D [ApoD]). Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed using urine samples from the same patient and control groups analyzed in the previous proteomics study (18 AD and 18 controls, set 1) and urine samples from an independent group of AD patients and controls (13 AD, 5 mild cognitive impairment [MCI], and 32 controls) from the National Center for Geriatrics and Gerontology Biobank (set 2). Results: In set 1, the crude urinary levels of ApoD, Igfbp3, and creatinine-adjusted ApoD were significantly higher in the AD group relative to the control group (p = 0.003, p = 0.002, and p = 0.019, respectively), consistent with our previous proteomics results. In set 2, however, the crude urinary levels of Igfbp3 were significantly lower in the AD+MCI group than in the control group (p = 0.028), and the levels of ApoD and ApoC3 did not differ significantly compared to the control group. Combined analysis of all samples revealed creatinine-adjusted ApoC3 levels to be significantly higher in the AD+MCI group (p = 0.015) and the AD-only group (p = 0.011) relative to the control group. Conclusion: ApoC3 may be a potential biomarker for AD, as validated by ELISA. Further analysis of ApoC3 as a urinary biomarker for AD is warranted.

    DOI: 10.1159/000509561

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  • Molecular Network Analysis of the Urinary Proteome of Alzheimer's Disease Patients. 査読

    Watanabe Y, Hirao Y, Kasuga K, Tokutake T, Semizu Y, Kitamura K, Ikeuchi T, Nakamura K, Yamamoto T

    Dementia and geriatric cognitive disorders extra   9 ( 1 )   53 - 65   2019年1月

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    担当区分:筆頭著者  

    DOI: 10.1159/000496100

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  • Association between dialysis treatment and cognitive decline: A study from the Project in Sado for Total Health (PROST), Japan 査読

    Yumi Watanabe, Kaori Kitamura, Kazutoshi Nakamura, Kazuhiro Sanpei, Minako Wakasugi, Akio Yokoseki, Keiko Kabasawa, Osamu Onodera, Takeshi Ikeuchi, Ryozo Kuwano, Takeshi Momotsu, Ichici Narita, Naoto Endo

    GERIATRICS & GERONTOLOGY INTERNATIONAL   17 ( 10 )   1584 - 1587   2017年10月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Aim: Evidence for the association between dialysis treatment and cognitive decline is limited. The present study aimed to determine whether dialysis treatment is associated with cognitive decline in adult outpatients of a general hospital in Japan.
    Methods: This was a cross-sectional substudy of the Project in Sado for Total Etealth (PROST). Total Etealth PROST targeted adult outpatients of a general hospital in Sado City, Niigata, Japan. Among 753 patients (mean age 68.1 11.6 years) analyzed, 66 received dialysis. Cognitive state was evaluated using the Mini-Mental State Examination, and those with a Mini-Mental State Examination score <24 were considered "cognitively declined." The prevalence of cognitive decline was compared by odds ratios calculated with multiple logistic regression analysis. Variables included in the analyses were dialysis, age, sex and self-reported histories of hypertension, diabetes, stroke and ischemic heart disease.
    Results: Of the 66 dialysis patients, 24 (36.4%) showed cognitive decline, whereas 172 (25.0%) of 687 non-dialysis patients showed cognitive decline. The age and sex-adjusted odds ratio for cognitive decline in dialysis patients was 2.57 (95% confidence interval 1.43-4.61), relative to non-dialysis patients. The odds ratio remained significant (odds ratio 2.69, 95% confidence interval 1.49-4.88) even after adjusting for all covariates.
    Conclusion: The prevalence of cognitive decline was high in dialysis patients relative to non-dialysis patients among outpatients of a general hospital in Japan..

    DOI: 10.1111/ggi.129.7

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  • Elevated C-Reactive Protein Is Associated with Cognitive Decline in Outpatients of a General Hospital: The Project in Sado for Total Health (PROST). 査読

    Watanabe Y, Kitamura K, Nakamura K, Sanpei K, Wakasugi M, Yokoseki A, Onodera O, Ikeuchi T, Kuwano R, Momotsu T, Narita I, Endo N

    Dementia and geriatric cognitive disorders extra   6 ( 1 )   10 - 19   2016年1月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1159/000442585

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  • Point Mutation in Syntaxin-1A Causes Abnormal Vesicle Recycling, Behaviors, and Short Term Plasticity 査読

    Yumi Watanabe, Norikazu Katayama, Kosei Takeuchi, Tetsuya Togano, Rieko Itoh, Michiko Sato, Maya Yamazaki, Manabu Abe, Toshiya Sato, Kanako Oda, Minesuke Yokoyama, Keizo Takao, Masahiro Fukaya, Tsuyoshi Miyakawa, Masahiko Watanabe, Kenji Sakimura, Toshiya Manabe, Michihiro Igarashi

    JOURNAL OF BIOLOGICAL CHEMISTRY   288 ( 48 )   34906 - 34919   2013年11月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Background: Roles of the syntaxin-1ACaMKII interaction are not physiologically understood in vivo.Results: A point mutation in syntaxin-1A caused abnormal plasticity, recycling, and behaviors in mice. Conclusion: The CaMKII/syntaxin-1A interaction is essential for maintenance of neuronal plasticity. Significance: Syntaxin-1A is involved in regulatory pathways in higher brain functions.
    Syntaxin-1A is a t-SNARE that is involved in vesicle docking and vesicle fusion; it is important in presynaptic exocytosis in neurons because it interacts with many regulatory proteins. Previously, we found the following: 1) that autophosphorylated Ca2+/calmodulin-dependent protein kinase II (CaMKII), an important modulator of neural plasticity, interacts with syntaxin-1A to regulate exocytosis, and 2) that a syntaxin missense mutation (R151G) attenuated this interaction. To determine more precisely the physiological importance of this interaction between CaMKII and syntaxin, we generated mice with a knock-in (KI) syntaxin-1A (R151G) mutation. Complexin is a molecular clamp involved in exocytosis, and in the KI mice, recruitment of complexin to the SNARE complex was reduced because of an abnormal CaMKII/syntaxin interaction. Nevertheless, SNARE complex formation was not inhibited, and consequently, basal neurotransmission was normal. However, the KI mice did exhibit more enhanced presynaptic plasticity than wild-type littermates; this enhanced plasticity could be associated with synaptic response than did wild-type littermates; this pronounced response included several behavioral abnormalities. Notably, the R151G phenotypes were generally similar to previously reported CaMKII mutant phenotypes. Additionally, synaptic recycling in these KI mice was delayed, and the density of synaptic vesicles was reduced. Taken together, our results indicated that this single point mutation in syntaxin-1A causes abnormal regulation of neuronal plasticity and vesicle recycling and that the affected syntaxin-1A/CaMKII interaction is essential for normal brain and synaptic functions in vivo.

    DOI: 10.1074/jbc.M113.504050

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  • Leisure-Time and Non-Leisure-Time Physical Activities are Dose-Dependently Associated With a Reduced Risk of Dementia in Community-Dwelling People Aged 40-74 Years: The Murakami Cohort Study. 国際誌

    Kaori Kitamura, Yumi Watanabe, Keiko Kabasawa, Akemi Takahashi, Toshiko Saito, Ryosaku Kobayashi, Ribeka Takachi, Rieko Oshiki, Shoichiro Tsugane, Masayuki Iki, Ayako Sasaki, Osamu Yamazaki, Kei Watanabe, Kazutoshi Nakamura

    Journal of the American Medical Directors Association   23 ( 7 )   1197 - 1204   2022年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: Although physical activity (PA) in late life is considered a preventive factor for dementia, effects of different types of PAs on the development of dementia in early old age are unclear. This study aimed to determine the effect of leisure-time and non-leisure-time PAs on dementia risk in middle-aged and older adults during an 8-year follow-up. DESIGN: Prospective cohort study. SETTING AND PARTICIPANTS: Participants were 13,773 community-dwelling individuals aged 40-74 years who completed the baseline self-administered questionnaire survey of the Murakami cohort study in 2011-2013. METHODS: Main predictors were leisure-time and non-leisure-time (commute, occupational work, and housework) PAs as assessed by MET score (MET-hour/d). The outcome was newly developed dementia determined using a long-term care insurance database. Covariates included demographics, lifestyle, body size, disease history, and PA level. Hazard ratios (HRs) were calculated using Cox proportional hazards models. RESULTS: Mean age of participants was 59.0 (SD 9.3) years. Higher levels of leisure-time PA were associated with lower HRs (adjusted P for trend <.001), with all tertiles having significantly lower HRs (low: 0.71, 95% CI 0.51-0.99; medium: 0.59, 95% CI 0.43-0.81; high: 0.55, 95% CI 0.41-0.75) relative to the reference (zero). Higher quartiles of non-leisure-time PA were associated with lower adjusted HRs for dementia (adjusted P for trend < .001), with the second-fourth quartiles having significantly lower HRs (second: 0.73, 95% CI 0.54-0.98; third: 0.59, 95% CI 0.43-0.81; fourth: 0.55, 95% CI 0.41-0.75) relative to the lowest quartile. These associations were robust regardless of sex and age group. CONCLUSIONS AND IMPLICATIONS: Both leisure-time and non-leisure-time PAs are independently and robustly associated with a reduced risk of dementia.

    DOI: 10.1016/j.jamda.2022.01.053

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  • Association between Sarcopenia and Depressive Symptoms in Community-Dwelling People Aged 40 Years and Older.

    Alena Zakharova, Keiko Kabasawa, Yumi Ito, Junta Tanaka, Aya Hinata, Kaori Kitamura, Yumi Watanabe, Shoichiro Tsugane, Kazutoshi Nakamura, Ichiei Narita

    The Tohoku journal of experimental medicine   257 ( 2 )   117 - 125   2022年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Several studies have reported an association between sarcopenia and depression. Their results, however, are inconsistent, partly due to small sample sizes and lack of consideration of important confounders. The present study aimed to cross-sectionally examine this association in community-dwelling people in Japan. This study used baseline data from the Yuzawa cohort study (age ≥ 40 years), with the final analysis population comprising 2,466 participants. A self-administered questionnaire was used to elicit information related to sarcopenia, depressive symptoms, demographic characteristics, anthropometrics, disease history, and lifestyles. Sarcopenia was diagnosed using SARC-F, a validated questionnaire including components of Strength, Assistance in walking, Rising from a chair, Climbing stairs, and Falls. Depressive symptoms were assessed using the 11-item version of the Center for Epidemiologic Studies Depression Scale (CES-D). For depressive symptoms, prevalence ratios (PRs) were calculated, and odds ratio (ORs) were obtained using simple and multiple logistic regression analyses. Mean age of participants was 61.7 years (standard deviation = 11.8), and 10.5% and 34.7% had sarcopenia and depressive symptoms, respectively. Sarcopenic individuals had a significantly higher PR (2.00), unadjusted OR (3.67), and adjusted OR (4.96) compared to non-sarcopenic individuals, with an estimated adjusted PR of 2.7. There was a significant dose-dependent association between SARC-F scores and depressive symptoms in sarcopenic individuals (adjusted P for trend = 0.0028). In conclusion, sarcopenia and depressive symptoms were robustly associated in community-dwelling, middle-aged and older people in Japan. However, the direction of this association is unclear, and a future cohort study will be needed to determine causality.

    DOI: 10.1620/tjem.2022.J024

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  • Secular changes in bone mineral density of adult Japanese women from 1995 to 2013.

    Hiroaki Watanabe, Yasuko Minagawa, Ichiro Suzuki, Kaori Kitamura, Yumi Watanabe, Keiko Kabasawa, Kseniia Platonova, Aya Hinata, Kazutoshi Nakamura

    Fukushima journal of medical science   67 ( 3 )   128 - 134   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    INTRODUCTION: Secular changes in hip fracture incidence have been reported in the last few decades in Japan, but whether long-term bone mineral density (BMD) is also changing is unclear. This study aimed to determine whether BMD of Japanese women has changed over time. METHODS: Subjects were 10,649 adult women who underwent BMD measurement in a health check-up population in Niigata, Japan, between 1995 and 2013. BMD of the distal, non-dominant forearm was measured by dual-energy X-ray absorptiometry. Demographic information and BMI were also obtained. Secular trends were determined by linear regression analysis. RESULTS: BMD of subjects in their 40's decreased significantly in the age-adjusted model (P for trend=0.0162), but not in the age- and BMI-adjusted model (P for trend=0.2171). BMD of subjects in their 50's decreased marginally in the age-adjusted model (P for trend=0.0535), but not in the age- and BMI-adjusted model (P for trend=0.6601). BMDs of subjects in their 30's and 60's did not significantly change, while BMIs of subjects in their 40's-60's decreased significantly. CONCLUSIONS: A secular decrease in BMD, partly attributed to decreases in BMI, was observed in middle-aged Japanese women from 1995 to 2013. Measures to help maintain suitable BMI will be necessary to prevent a decrease in BMD among women.

    DOI: 10.5387/fms.2021-10

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  • Serum 25-hydroxyvitamin D levels are not associated with impaired postural sway in community-dwelling older women: a 6-year follow-up study. 国際誌

    Kazutoshi Nakamura, Toshiko Saito, Akemi Takahashi, Ryosaku Kobayashi, Rieko Oshiki, Kaori Kitamura, Yumi Watanabe

    Journal of musculoskeletal & neuronal interactions   21 ( 4 )   501 - 508   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: A positive association between levels of blood 25-hydroxyvitamin D (25[OH]D), an index of vitamin D status, and physical balance has been reported from cross-sectional studies, but longitudinal studies are rare. The present study aimed to test the hypothesis that low serum 25(OH)D levels are longitudinally associated with impaired postural sway over a 6-year follow-up period in older women. METHODS: The present cohort consisted of 392 community-dwelling Japanese women aged ≥69 years. Baseline examinations included serum 25(OH)D and physical performance tests, including postural sway velocity. Standing postural sway was evaluated by measuring gravity-center sway velocity. Follow-up physical performance tests were conducted 6 years later. RESULTS: Mean subject age and serum 25(OH)D levels were 73.3 years (SD 3.7) and 61.0 nmol/L (SD 16.9), respectively. No significant association was found between 25(OH)D levels and changes in postural sway velocity (adjusted P for trend=0.72). Women with 25(OH)D <30 nmol/L tended to have lower Δpostural sway velocity than those with 25(OH)D ≥30 nmol/L (mean, -0.59 vs 0.37 cm/s, respectively; adjusted P=0.13). CONCLUSIONS: Vitamin D levels are not longitudinally associated with impaired postural sway in older women. Further longitudinal studies are needed to corroborate the results of this study.

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  • Education, household income, and depressive symptoms in middle-aged and older Japanese adults. 国際誌

    Aya Hinata, Keiko Kabasawa, Yumi Watanabe, Kaori Kitamura, Yumi Ito, Ribeka Takachi, Shoichiro Tsugane, Junta Tanaka, Ayako Sasaki, Ichiei Narita, Kazutoshi Nakamura

    BMC public health   21 ( 1 )   2120 - 2120   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Income inequality has dramatically increased worldwide, and there is a need to re-evaluate the association between socio-economic status (SES) and depression. Relative contributions of household income and education to depression, as well as their interactions, have not been fully evaluated. This study aimed to examine the association between SES and depressive symptoms in Japanese adults, focusing on interactions between education and household income levels. METHODS: This cross-sectional study used data from baseline surveys of two cohort studies. Participants were 38,499 community-dwelling people aged 40-74 years who participated in baseline surveys of the Murakami cohort study (2011-2012) and Uonuma cohort study (2012-2015) conducted in Niigata Prefecture, Japan. Information regarding marital status, education level, household income, occupation, activities of daily living (ADL), and history of cancer, myocardial infarction, stroke, and diabetes was obtained using a self-administered questionnaire. Depressive symptoms were examined using the Center for Epidemiologic Studies Depression Scale (CES-D). Logistic regression analysis was used to obtain odds ratios (ORs). Covariates included age, sex, marital status, education, household income, occupation, ADL, and disease history. RESULTS: Individuals with higher education levels had lower ORs (adjusted P for trend = 0.0007) for depressive symptoms, independently of household income level. The OR of the university-or-higher group was significantly lower than that of the junior high school group (adjusted OR = 0.79). Individuals with lower household income levels had higher ORs (adjusted P for trend< 0.0001) for depressive symptoms, independently of education level. The type of occupation was not associated with depressive symptoms. In subgroup analyses according to household income level, individuals with higher education levels had significantly lower ORs in the lowest- and lower-income groups (adjusted P for trend = 0.0275 and 0.0123, respectively), but not in higher- and highest-income groups (0.5214 and 0.0915, respectively). CONCLUSIONS: Both education and household income levels are independently associated with the prevalence of depressive symptoms, with household income levels showing a more robust association with depressive symptoms than education levels. This suggests that a high household income level may offset the risk of depressive symptoms from having a low education level.

    DOI: 10.1186/s12889-021-12168-8

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  • Association of coffee, green tea, and caffeine with the risk of dementia in older Japanese people. 査読 国際誌

    Nana Matsushita, Yuta Nakanishi, Yumi Watanabe, Kaori Kitamura, Keiko Kabasawa, Akemi Takahashi, Toshiko Saito, Ryosaku Kobayashi, Ribeka Takachi, Rieko Oshiki, Shoichiro Tsugane, Masayuki Iki, Ayako Sasaki, Osamu Yamazaki, Kei Watanabe, Kazutoshi Nakamura

    Journal of the American Geriatrics Society   2021年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: Coffee, green tea, and caffeine are potential preventive factors for dementia, but the underlying evidence is insufficient. This study aimed to examine associations between the consumption of coffee, green tea, and caffeine and dementia risk in middle-aged and older people. METHODS: This was a cohort study with an 8.0-year follow-up. Participants were community-dwelling individuals (n = 13,757) aged 40-74 years. A self-administered questionnaire survey was conducted in 2011-2013. Predictors were the consumption of coffee/green tea, from which caffeine consumption was estimated. The outcome was incident dementia obtained from the long-term care insurance database. Covariates were demographic factors, body mass index, physical activity, energy, smoking, drinking, and disease history. Adjusted hazard ratios (HRs) were calculated using Cox proportional hazards models. HRs were also calculated using a Cox model with delayed entry. RESULTS: The number of dementia cases during the study period was 309. Participants with higher coffee consumption had lower HRs (adjusted p for trend = 0.0014), with the fifth quintile (≥326 ml/day) having a significantly lower HR (0.49, 95% confidence interval [CI]: 0.30-0.79) than the first quintile (<26 ml/day, reference). Similarly, participants with higher caffeine consumption had a significantly lower HR (adjusted p for trend = 0.0004) than the reference. The Cox model with delayed entry yielded similar results. These associations were significant in men, but not in women. Moreover, participants who consumed 2-2.9 cups/day and ≥3 cups/day of coffee had lower HRs (0.69, 95% CI: 0.48-0.98 and 0.53, 95% CI: 0.31-0.89, respectively) than those who consumed 0 cup/day. The association between green tea consumption and reduced dementia risk was significant (adjusted p for trend = 0.0146) only in the 60-69 years age subgroup. CONCLUSIONS: High levels of coffee and caffeine consumption were significantly associated with a reduced dementia risk in a dose-dependent manner, especially in men. Moreover, coffee consumption of ≥3 cups/day was associated with a 50% reduction in dementia risk.

    DOI: 10.1111/jgs.17407

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  • Menstrual and reproductive factors and limitations in activities of daily living: A case-control study within the Japan Public Health Center-based Prospective Study. 査読 国際誌

    Kazutoshi Nakamura, Norie Sawada, Kaori Kitamura, Yumi Watanabe, Shoichiro Tsugane

    The journal of obstetrics and gynaecology research   47 ( 11 )   3903 - 3912   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    AIM: Although menstrual/reproductive factors are known to be associated with physical disability, little is known about these associations in relation to activities of daily living (ADL). This study aimed to clarify associations between menstrual/reproductive factors and ADL limitations in peri- and postmenopausal women. STUDY DESIGN: A nested case-control study of the Japan Public Health Center-based Prospective (JPHC) Study. METHODS: The main outcome measure was self-reported ADL levels in the 10-year follow-up questionnaire survey of the JPHC Study conducted between 2000 and 2004 (N = 36 460). Women who "live inside almost independently, but go out with assistance" or had a lower level of activity were considered to have ADL limitations ("cases"), and all others served as controls. Candidate menstrual/reproductive predictors were as follows: menarcheal age, menopausal status, menopausal age, regularity of menses, menstrual cycle, number of pregnancies, age at first pregnancy, number of deliveries, age at first delivery, and breast feeding. Multiple logistic regression analyses were conducted, and odds ratios adjusted for age and past lifestyle were calculated. RESULTS: Mean ages of cases (N = 592) and controls (N = 38 656) were 68.3 (SD = 7.6) and 61.1 (SD = 7.7) years, respectively. With respect to menopausal age, groups aged <45 and ≥55 years had significantly higher adjusted ORs (1.44, 95% CI: 1.09-1.90 and 1.55, 95%CI: 1.09-2.18, respectively) than the reference group (50-54 years). Multiparous women had significantly lower ORs than nulliparous women. CONCLUSION: Our findings suggest that menopausal age and parity may predict future ADL limitations in women.

    DOI: 10.1111/jog.14959

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  • Short daytime napping reduces the risk of cognitive decline in community-dwelling older adults: a 5-year longitudinal study. 査読 国際誌

    Kaori Kitamura, Yumi Watanabe, Kazutoshi Nakamura, Chikako Takano, Naomi Hayashi, Hisami Sato, Toshiyuki Someya

    BMC geriatrics   21 ( 1 )   474 - 474   2021年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Beneficial effects of napping on cognition have been suggested in cross-sectional studies. This study aimed to clarify longitudinal associations between cognitive decline and sleep characteristics, particularly daytime napping, over a 5-year period in older adults. METHODS: Study participants were 389 community-dwelling individuals aged ≥65 years living in Ojiya City, Niigata, Japan. Baseline and follow-up examinations were conducted in 2011-2013 and 2016-2018, respectively. Trained nurses visited and interviewed participants to collect the following information at baseline and follow-up: demographic characteristics, disease history, lifestyle habits including bedtime, sleeping hours, and daytime nap duration, and cognitive function. The assessment of cognitive function was performed using the revised Hasegawa's dementia scale (HDS-R), with cognitive decline defined as a change in the HDS-R of ≤ - 3 over 5 years. Odds ratios (ORs) for cognitive decline were calculated using multiple logistic regression analysis. RESULTS: Mean age of participants was 74.6 years (SD 6.4), and the cumulative incidence of cognitive decline was 106/389 (27.3%). The adjusted OR for 1-29 min daytime napping was significantly lower compared to that for no napping (OR = 0.47, 95%CI: 0.23-0.96). Earlier bedtime was associated with cognitive decline (adjusted P for trend = 0.0480). CONCLUSION: Short daytime napping (< 30 min) reduces the risk of cognitive decline over 5 years for community-dwelling older people. A future study will be necessary to confirm the effect of short napping on the reduction of risk for clinically diagnosed dementia.

    DOI: 10.1186/s12877-021-02418-0

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  • Body mass index and risk of recurrent falls in community‐dwelling Japanese aged 40–74 years: The Murakami cohort study 査読

    Kazutoshi Nakamura, Kaori Kitamura, Yumi Watanabe, Keiko Kabasawa, Akemi Takahashi, Aya Hinata, Toshiko Saito, Ryosaku Kobayashi, Rieko Oshiki, Ribeka Takachi, Shoichiro Tsugane, Masayuki Iki, Ayako Sasaki, Osamu Yamazaki, Kei Watanabe

    Geriatrics & Gerontology International   21 ( 6 )   498 - 505   2021年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    AIMS: A prior meta-analysis found that obesity (body mass index [BMI] ≥ 30 kg/m2 ) was associated with a high fall risk, while being overweight (BMI≥25, <30 kg/m2 ) was associated with the lowest fall risk. However, whether these associations hold true for East Asians is unknown. This study aimed to assess the association between BMI and incidence of recurrent falls in Japanese aged 40-74 years. METHODS: This 5-year follow-up cohort study involved 7538 community-dwelling individuals who did not experience recurrent falls in the year before the baseline study. Information on demographics, body size, lifestyle, and disease history was obtained using a self-administered questionnaire. BMI was categorized as <18.5 (underweight), 18.5-20.6 (low-normal), 20.7-22.7 (mid-normal, reference), 22.8-24.9 (high-normal), and ≥ 25.0 kg/m2 (overweight). The outcome was recurrent falls reported, and fall history in the previous year was recorded as none, once, or twice or more (recurrent falls). RESULTS: Mean BMI was 23.5 kg/m2 (SD 2.9) for men and 22.7 kg/m2 (SD 3.2) for women. The adjusted odds ratio (OR) for recurrent falls in the BMI ≥25 group was significantly higher (1.41, 95%CI: 1.02-1.93) than that in the reference group. The adjusted OR in the BMI ≥25 group was significantly higher than that in the reference group for the age ≥ 60 subgroup (1.62, 95%CI: 1.09-2.40), but not for the age < 60 subgroup (OR = 1.04, 95%CI: 0.60-1.80). CONCLUSIONS: Being overweight may be a risk factor for recurrent falls in community-dwelling older Japanese. Further studies are needed to determine the underlying mechanism. Geriatr Gerontol Int 2021; 21: 498-505.

    DOI: 10.1111/ggi.14167

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/ggi.14167

  • Dietary calcium and vitamin K are associated with osteoporotic fracture risk in middle-aged and elderly Japanese women, but not men: the Murakami Cohort Study. 査読 国際誌

    Kseniia Platonova, Kaori Kitamura, Yumi Watanabe, Ribeka Takachi, Toshiko Saito, Keiko Kabasawa, Akemi Takahashi, Ryosaku Kobayashi, Rieko Oshiki, Aleksandr Solovev, Masayuki Iki, Shoichiro Tsugane, Ayako Sasaki, Osamu Yamazaki, Kei Watanabe, Kazutoshi Nakamura

    The British journal of nutrition   125 ( 3 )   319 - 328   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Although dietary Ca, vitamin D and vitamin K are nutritional factors associated with osteoporosis, little is known about their effects on incident osteoporotic fractures in East Asian populations. This study aimed to determine whether intakes of these nutrients predict incident osteoporotic fractures. We adopted a cohort study design with a 5-year follow-up. Subjects were 12 794 community-dwelling individuals (6301 men and 6493 women) aged 40-74 years. Dietary intakes of Ca, vitamin D and vitamin K were assessed with a validated FFQ. Covariates were demographic and lifestyle factors. All incident cases of major osteoporotic limb fractures, including those of the distal forearm, neck of humerus, neck or trochanter of femur and lumbar or thoracic spine were collected. Hazard ratios (HR) for energy-adjusted Ca, vitamin D and vitamin K were calculated with the residual method. Mean age was 58·8 (sd 9·3) years. Lower energy-adjusted intakes of Ca and vitamin K in women were associated with higher adjusted HR of total fractures (Pfor trend = 0·005 and 0·08, respectively). When vertebral fracture was the outcome, Pfor trend values for Ca and vitamin K were 0·03 and 0·006, respectively, and HR of the lowest and highest (reference) intake groups were 2·03 (95 % CI 1·08, 3·82) and 2·26 (95 % CI 1·19, 4·26), respectively. In men, there were null associations between incident fractures and each of the three nutrient intakes. Lower intakes of dietary Ca and vitamin K were independent lifestyle-related risk factors for osteoporotic fracture in women but not men. These associations were robust for vertebral fractures, but not for limb fractures.

    DOI: 10.1017/S0007114520001567

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  • Predictors of decline in vitamin D status in middle-aged and elderly individuals: a 5-year follow-up study. 査読 国際誌

    Kazutoshi Nakamura, Kaori Kitamura, Yumi Watanabe, Toshiko Saito, Akemi Takahashi, Ryosaku Kobayashi, Rieko Oshiki, Keiko Kabasawa, Ribeka Takachi, Shoichiro Tsugane, Masayuki Iki, Ayako Sasaki, Kei Watanabe

    The British journal of nutrition   124 ( 7 )   729 - 735   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Little is known about predictors of decline in vitamin D status (vitamin D decline) over time. We aimed to determine demographic and lifestyle variables associated with vitamin D decline by sufficiently controlling for seasonal effects of vitamin D uptake in a middle-aged to elderly population. Using a longitudinal study design within the larger framework of the Murakami Cohort Study, we examined 1044 individuals aged between 40 and 74 years, who provided blood samples at baseline and at 5-year follow-up, the latter of which were taken on a date near the baseline examination (±14 d). Blood 25-hydroxyvitamin D (25(OH)D) concentrations were determined with the Liaison® 25OH Vitamin D Total Assay. A self-administered questionnaire collected demographic, body size and lifestyle information. Vitamin D decline was defined as the lowest tertile of 5-year changes in blood 25(OH)D (Δ25(OH)D) concentration (<6·7 nmol/l). Proportions of those with vitamin D decline were 182/438 (41·6 %) in men and 166/606 (27·4 %) in women (P < 0·0001). In men, risk of vitamin D decline was significantly lower in those with an outdoor occupation (P = 0·0099) and those with the highest quartile of metabolic equivalent score (OR 0·34; 95 % CI 0·14, 0·83), and higher in those with 'university or higher' levels of education (OR 2·92; 95 % CI 1·04, 8·19). In women, risk of vitamin D decline tended to be lower with higher levels of vitamin D intake (Pfor trend = 0·0651) and green tea consumption (Pfor trend = 0·0025). Predictors of vitamin D decline differ by sex, suggesting that a sex-dependent intervention may help to maintain long-term vitamin D levels.

    DOI: 10.1017/S0007114520001580

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  • Total physical activity and risk of chronic low back and knee pain in middle-aged and elderly Japanese people: The Murakami cohort study. 査読 国際誌

    Aleksandr Solovev, Yumi Watanabe, Kaori Kitamura, Akemi Takahashi, Ryosaku Kobayashi, Toshiko Saito, Ribeka Takachi, Keiko Kabasawa, Rieko Oshiki, Kseniia Platonova, Shoichiro Tsugane, Masayuki Iki, Ayako Sasaki, Osamu Yamazaki, Kei Watanabe, Kazutoshi Nakamura

    European journal of pain (London, England)   24 ( 4 )   863 - 872   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Specific components of physical activity, such as vigorous exercise and heavy occupational work, are known to increase the risk of chronic low back pain (CLBP) and chronic knee pain (CKP), but impacts of other components are less known. This study aimed to assess the relationship between total physical activity and risk of CLBP and CKP from a public health perspective. METHODS: Participants were 7,565 individuals, aged 40-74 years, who did not have CLBP or CKP, and who participated in the 5-year follow-up survey. A self-administered questionnaire was used to obtain information on demographics, body size and lifestyle (including physical activity) in the baseline survey in 2011-2013, and on CLBP and CKP using Short Form 36 (SF-36) in the follow-up survey. Sitting, standing, walking and strenuous work for occupational activity were assessed for total physical activity, and walking slowly, walking quickly, light to moderate exercise and strenuous exercise were assessed for leisure-time physical activity using metabolic equivalent hours/day (METs score). RESULTS: Mean age of participants was 60.1 years (SD, 8.8). Participants with higher METs scores had a significantly higher risk of CKP (p for trend = 0.0089, OR of 4th quartile = 1.29, 95% CI: 1.04-1.59 vs. 1st quartile), but not CLBP. An intermediate leisure-time METs score was associated with a lower risk of CLBP (OR = 0.75, 95%CI: 0.61-0.92 vs. 0 METs-group). CONCLUSIONS: A high level of total physical activity may increase the risk of CKP, whereas an intermediate level of leisure-time physical activity may decrease the risk of CLBP, in middle-aged and elderly individuals. SIGNIFICANCE: Evidence on the longitudinal association between total physical activity and CLBP and CKP in middle-aged and elderly people is lacking. We conducted a cohort study to assess this association, and found that high levels of total physical activity increased risk of CKP, and intermediate levels of leisure-time physical activity decreased risk of CLBP. This suggests that the effect of physical activity on chronic pain differed by pain site.

    DOI: 10.1002/ejp.1535

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  • Low serum 25-hydroxyvitamin D is associated with low grip strength in an older Japanese population. 査読

    Taeko Kitsu, Keiko Kabasawa, Yumi Ito, Kaori Kitamura, Yumi Watanabe, Junta Tanaka, Kazutoshi Nakamura, Ichiei Narita

    Journal of bone and mineral metabolism   38 ( 2 )   198 - 204   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Positive associations between vitamin D levels and hand grip strength have been reported worldwide, but the results are not consistent and few studies on East Asian populations have been published. The aim of this study was to determine whether such an association is present in a community-dwelling Japanese population, including elderly and middle-aged individuals. This study used a cross-sectional design. Participants were 492 community-dwelling individuals aged ≥ 40 years living in Yuzawa Town, Japan. The health check examination was conducted in 2015, and serum 25-hydroxyvitamin D [25(OH)D, an index of vitamin D levels], and hand grip strength were measured. Covariates were serum albumin concentration, body mass index, and physical activity level. The associations of serum 25(OH)D concentrations with hand grip strength and low grip strength (< 26 kg for men and < 18 kg for women) were analyzed using analysis of covariance and multiple logistic regression. Mean (standard deviation) age and serum 25(OH)D were 75.4 (9.0) years and 30.9 (9.1) ng/mL, respectively. The prevalence of serum 25(OH)D < 20, 20-29, and ≥ 30 ng/mL was 7.3%, 37.8%, and 54.9%, respectively. Mean hand grip strength in the 25(OH)D < 20 ng/mL group was significantly lower than that in the ≥ 30 ng/mL group (adjusted P ≤ 0.001). The 25(OH)D < 20 ng/mL group was significantly more likely to have low grip strength than the 25(OH)D ≥ 30 ng/mL group (odds ratio = 4.12). In conclusion, low serum 25(OH)D concentration (< 20 ng/mL) is associated with low grip strength in an older Japanese population.

    DOI: 10.1007/s00774-019-01040-w

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  • Psychological distress as a risk factor for dementia after the 2004 Niigata-Chuetsu earthquake in Japan. 査読 国際誌

    Kazutoshi Nakamura, Yumi Watanabe, Kaori Kitamura, Keiko Kabasawa, Toshiyuki Someya

    Journal of affective disorders   259   121 - 127   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: A large earthquake can cause extreme stress and may adversely affect cognitive function in humans. We aimed to examine a possible association between psychological distress and incident dementia after the 2004 Niigata-Chuetsu earthquake in Japan. METHODS: This is a retrospective cohort study followed participants for 10-12 years. Subjects were 6,012 residents in 2005, 5,424 in 2006, and 5,687 in 2007 (age ≥40 years) living in Ojiya city who participated in the annual health check examinations after the 2004 Niigata-Chuetsu earthquake. Psychological distress was assessed using the Kessler Psychological Distress Scale (K10), and individuals with a K10 score ≥10 were considered to have psychological distress. Incident dementia cases were identified from a long-term care insurance database of the local government during the follow-up period. We evaluated hazard ratios (HRs) of psychological distress for incident dementia in each year, unadjusted and adjusted for covariates, including sex, age, occupation, BMI, and property damage of residential area. RESULTS: The average age of the subjects was 64.6 years in 2005, 64.6 in 2006, and 65.2 in 2007. Adjusted HRs were significantly higher (HR = 1.20-1.66) in the psychological distress group than in the reference group in each year. In particular, adjusted HR was high (HR = 2.89) in those with psychological distress in all three years (2005-2007). CONCLUSION: Psychological distress, especially persistent distress, is a risk factor for incident dementia in victims of large disasters.

    DOI: 10.1016/j.jad.2019.08.041

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  • Modifiable factors associated with symptomatic knee osteoarthritis: The Murakami cohort study. 査読 国際誌

    Ryoya Takiguchi, Rintaro Komatsu, Kaori Kitamura, Yumi Watanabe, Akemi Takahashi, Ryosaku Kobayashi, Rieko Oshiki, Toshiko Saito, Keiko Kabasawa, Ribeka Takachi, Shoichiro Tsugane, Masayuki Iki, Ayako Sasaki, Osamu Yamazaki, Kazutoshi Nakamura

    Maturitas   128   53 - 59   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: Modifiable risk factors for knee osteoarthritis (OA) have not been studied in detail. This study aimed to determine lifestyle-related modifiable factors of symptomatic knee osteoarthritis in an East Asian population. STUDY DESIGN: This 5-year cohort study involved 11,091 individuals (age range 40-72 years) living in the Murakami region of Niigata, Japan, who did not have a history of knee OA. At baseline, information on sociodemographic characteristics, body size, lifestyle, and living condition was obtained using a self-administered questionnaire. MAIN OUTCOME MEASURES: Incident symptomatic knee OA observed at hospitals and orthopaedic clinics in the five years to 2016. Clinical grades of knee OA were based on the Kellgren-Lawrence scale. P for trend was assessed to examine linear associations between predictors and the outcome in multiple logistic regression analysis. RESULTS: The mean age of participants was 58.1 (SD 9.3) years. The number of cases of grade 2 or more incident knee OA was 429. In men, older age (P for trend < 0.0001), higher BMI (P for trend < 0.0001), higher METs score (P for trend = 0.0150), less smoking (P for trend = 0.0249), and lower green tea consumption (P for trend = 0.0437) were associated with incident knee OA. In women, older age (P for trend < 0.0001), higher BMI (P for trend < 0.0001), and alcohol consumption (P = 0.0153) were associated with incident knee OA. CONCLUSIONS: Several lifestyle-related factors were found to be associated with incident knee OA and exhibited sex-dependent differences. In particular, higher consumption of green tea was associated with a lower incidence of knee OA in men.

    DOI: 10.1016/j.maturitas.2019.06.013

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  • Physical activity modifies the effect of calcium supplements on bone loss in perimenopausal and postmenopausal women: subgroup analysis of a randomized controlled trial. 査読

    Nakamura K, Saito T, Kobayashi R, Oshiki R, Kitamura K, Watanabe Y

    Archives of osteoporosis   14 ( 1 )   17   2019年2月

  • Menstrual and reproductive factors and risk of vertebral fractures in Japanese women: the Japan Public Health Center-based prospective (JPHC) study.Menstrual and reproductive factors and risk of vertebral fractures in Japanese women: the Japan Public Heal 査読

    Shimizu Y, Sawada N, Nakamura K, Watanabe Y, Kitamura K, Iwasaki M, Tsugane S, JPHC Study group

    Osteoporos Int   2018年8月

  • The Murakami Cohort Study of vitamin D for the prevention of musculoskeletal and other age-related diseases: a study protocol. 査読

    Nakamura K, Takachi R, Kitamura K, Saito T, Kobayashi R, Oshiki R, Watanabe Y, Kabasawa K, Takahashi A, Tsugane S, Iki M, Sasaki A, Yamazaki O

    Environmental health and preventive medicine   23 ( 1 )   28   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1186/s12199-018-0715-2

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  • Epidemiological profiles of chronic low back and knee pain in middle-aged and elderly Japanese from the Murakami cohort. 査読

    Takahashi A, Kitamura K, Watanabe Y, Kobayashi R, Saito T, Takachi R, Kabasawa K, Oshiki R, Tsugane S, Iki M, Sasaki A, Yamazaki O, Nakamura K

    Journal of pain research   11   3161 - 3169   2018年

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.2147/JPR.S184746

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  • Chondroitin Sulfate Is Required for Onset and Offset of Critical Period Plasticity in Visual Cortex 査読

    Xubin Hou, Nozomu Yoshioka, Hiroaki Tsukano, Akiko Sakai, Shinji Miyata, Yumi Watanabe, Yuchio Yanagawa, Kenji Sakimura, Kosei Takeuchi, Hiroshi Kitagawa, Takao K. Hensch, Katsuei Shibuki, Michihiro Igarashi, Sayaka Sugiyama

    SCIENTIFIC REPORTS   7 ( 1 )   12646   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Ocular dominance plasticity is easily observed during the critical period in early postnatal life. Chondroitin sulfate (CS) is the most abundant component in extracellular structures called perineuronal nets (PNNs), which surround parvalbumin-expressing interneurons (PV-cells). CS accumulates in PNNs at the critical period, but its function in earlier life is unclear. Here, we show that initiation of ocular dominance plasticity was impaired with reduced CS, using mice lacking a key CS-synthesizing enzyme, CSGalNAcT1. Two-photon in vivo imaging showed a weaker visual response of PV-cells with reduced CS compared to wild-type mice. Plasticity onset was restored by a homeoprotein Otx2, which binds the major CS-proteoglycan aggrecan and promotes its further expression. Continuous CS accumulation together with Otx2 contributed bidirectionally to both onset and offset of plasticity, and was substituted by diazepam, which enhances GABA function. Therefore, CS and Otx2 may act as common inducers of both onset and offset of the critical period by promoting PV-cell function throughout the lifetime.

    DOI: 10.1038/s41598-017-04007-x

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  • Weight loss from 20 years of age is associated with cognitive impairment in middle-aged and elderly individuals 査読

    Kaori Kitamura, Yumi Watanabe, Kazutoshi Nakamura, Akemi Takahashi, Ribeka Takachi, Rieko Oshiki, Ryosaku Kobayashi, Toshiko Saito, Shoichiro Tsugane, Ayako Sasaki

    PLOS ONE   12 ( 10 )   e0185960   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Background
    Few empirical studies have been conducted to identify modifiable factors that may affect cognitive impairment in Japanese individuals. The present study aimed to clarify whether body mass and lifestyle are associated with cognitive impairment in Japanese middle-aged and elderly individuals.
    Methods
    Subjects were 1814 community-dwelling individuals aged 44-79 years, all of whom were participants of the Murakami Cohort Study baseline survey conducted in 2011-2013. Cognitive function was assessed using the Mini-Mental State Examination (MMSE) in 2014-2016, and cognitive impairment, the outcome measure, was defined as an MMSE score &lt;24. Predictor variables were body mass index (BMI), long-term weight changes from 20 years of age, and lifestyle factors, such as smoking, drinking, and physical activity levels, which were obtained from a self-administered questionnaire in the baseline survey. Covariates were sex, age, education level, and histories of stroke and diabetes. Multiple logistic regression analysis was used to calculate the adjusted odds ratios (ORs).
    Results
    The prevalence of overall cognitive impairment was 6.2%. The adjusted ORs of cognitive impairment in the lowest (&lt;[-4] kg) (OR = 2.70, 95% CI, 1.18-6.20) and second ([-4]-[0] kg) (OR = 2.37, 95% CI, 1.04-5.37) quintiles for long-term weight change were significantly higher than the reference 4th quintile ([+4]-[+7] kg). The adjusted OR in the highest quintile &gt;=[+8] kg) was 2.24 (95% CI, 0.99-5.04). Current BMI was not associated with cognitive impairment.
    Conclusions
    Long-term weight loss is associated with cognitive impairment in Japanese middle-aged and elderly individuals. Because the present study was retrospective in nature, prospective studies should also be conducted for further characterization of this association.

    DOI: 10.1371/journal.pone.0185960

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  • Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis 査読

    Nozomu Yoshioka, Shinji Miyata, Atsushi Tamada, Yumi Watanabe, Asami Kawasaki, Hiroshi Kitagawa, Keizo Takao, Tsuyoshi Miyakawa, Kosei Takeuchi, Michihiro Igarashi

    MOLECULAR BRAIN   10 ( 1 )   47   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Chondroitin sulfate (CS) is an important glycosaminoglycan and is mainly found in the extracellular matrix as CS proteoglycans. In the brain, CS proteoglycans are highly concentrated in perineuronal nets (PNNs), which surround synapses and modulate their functions. To investigate the importance of CS, we produced and precisely examined mice that were deficient in the CS synthesizing enzyme, CSGalNAcT1 (T1KO). Biochemical analysis of T1KO revealed that loss of this enzyme reduced the amount of CS by approximately 50% in various brain regions. The amount of CS in PNNs was also diminished in T1KO compared to wild-type mice, although the amount of a major CS proteoglycan core protein, aggrecan, was not changed. In T1KO, we observed abnormalities in several behavioral tests, including the open-field test, acoustic startle response, and social preference. These results suggest that T1 is important for plasticity, probably due to regulation of CS-dependent PNNs, and that T1KO is a good model for investigation of PNNs.

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  • Rural-urban differences in the prevalence of cognitive impairment in independent community-dwelling elderly residents of Ojiya city, Niigata Prefecture, Japan 査読

    Kazutoshi Nakamura, Kaori Kitamura, Yumi Watanabe, Hiroko Shinoda, Hisami Sato, Toshiyuki Someya

    ENVIRONMENTAL HEALTH AND PREVENTIVE MEDICINE   21 ( 6 )   422 - 429   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    This study aimed to examine rural-urban differences in the prevalence of cognitive impairment in Japan.
    We targeted 592 residents aged 65 years and older who did not use long-term care insurance services in one rural and two urban areas in Ojiya City, Japan. Of these, 537 (90.7 %) participated in the study. The revised Hasegawa's dementia scale (HDS-R) was used to assess cognitive function, and cognitive impairment was defined as a HDS-R score aecurrency sign20. Lifestyle information was obtained through interviews. The prevalence of cognitive impairment was compared according to the levels of predictor variables by odds ratios (ORs) calculated by a logistic regression analysis.
    Mean age of participants was 75.7 years (SD 7.0). The prevalence of cognitive impairment was 20/239 (8.4 %) in the rural area and 6/298 (2.0 %) in the urban areas, for a total of 26/537 (4.8 %) overall. Men tended to have a higher prevalence of cognitive impairment (P = 0.0628), and age was associated with cognitive impairment (P for trend &lt; 0.0001). The rural area had a significantly higher prevalence of cognitive impairment (age- and sex-adjusted OR = 4.04, 95 % CI: 1.54-10.62) than urban areas. This difference was significant after adjusting for other lifestyle factors.
    The prevalence of cognitive impairment was higher in the rural area relative to urban areas in Ojiya city. This regional difference suggests the existence of potentially modifiable factors other than lifestyle in relation to cognitive impairment.

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  • Association between Dietary Intake and Bone Mineral Density in Japanese Postmenopausal Women: The Yokogoshi Cohort Study 査読

    Harumi Hirata, Kaori Kitamura, Toshiko Saito, Ryosaku Kobayashi, Masanori Iwasaki, Akihiro Yoshihara, Yumi Watanabe, Rieko Oshiki, Tomoko Nishiwaki, Kazutoshi Nakamura

    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE   239 ( 2 )   95 - 101   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TOHOKU UNIV MEDICAL PRESS  

    Diet and food intake play an important role in the development of osteoporosis. However, apart from calcium and vitamin D, how nutrients affect bone status is not fully understood. The purpose of this study was to determine cross-sectional and longitudinal associations between dietary intake and bone mineral density (BMD) in Japanese postmenopausal women. This 5-year cohort study included 600 community dwelling women aged 55-74 years at baseline in 2005. Information on demographics, nutrition, and lifestyle was obtained through interviews, and nutritional and dietary intake was assessed using a validated food frequency questionnaire. BMD measurements were performed by dual energy X-ray absorptiometry. In 2010, 498 women underwent follow-up BMD examinations. Multiple linear regression analysis was performed to determine associations of predictor variables with BMD, adjusting for confounders. In cross-sectional analyses, coffee or black tea consumption was positively associated with lumbar spine (P = 0.004) and total hip (P = 0.003) BMD, and alcohol intake was positively associated with femoral neck (P = 0.005) and total hip (P = 0.001) BMD. In longitudinal analyses, vitamin K (P = 0.028) and natto (fermented soybeans) (P = 0.023) were positively associated with lumbar spine BMD, and meat or meat product consumption was inversely associated with total hip (P = 0.047) BMD. In conclusion, dietary factors other than calcium and vitamin D intake are predictors of bone mass and bone loss in Japanese postmenopausal women. In particular, natto intake is recommended for preventing postmenopausal bone loss on the basis of current evidence.

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  • Modifiable Factors Associated with Cognitive Impairment in 1,143 Japanese Outpatients: The Project in Sado for Total Health (PROST). 査読

    Kitamura K, Watanabe Y, Nakamura K, Sanpei K, Wakasugi M, Yokoseki A, Onodera O, Ikeuchi T, Kuwano R, Momotsu T, Narita I, Endo N

    Dementia and geriatric cognitive disorders extra   6 ( 2 )   341 - 349   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1159/000447963

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  • Impact of demographic, environmental, and lifestyle factors on vitamin D sufficiency in 9084 Japanese adults 査読

    K. Nakamura, K. Kitamura, R. Takachi, T. Saito, R. Kobayashi, R. Oshiki, Y. Watanabe, S. Tsugane, A. Sasaki, O. Yamazaki

    BONE   74   10 - 17   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Background: Little is known about correlates of vitamin D status in Asian populations. In this study, we established the prevalence of vitamin D sufficiency in the Murakami region (latitude N38 degrees 13') in Niigata, Japan, and examined demographic, environmental, and lifestyle factors that might be associated with vitamin D sufficiency, with the aim of clarifying the relative contributions of previously described determinants of vitamin D status as well as identifying new determinants in this Japanese population.
    Methods: This study involved a cross-sectional analysis of baseline data obtained from a cohort study conducted in 2011-2013. Participants were 9084 individuals aged between 40 and 74 years who provided blood samples for the determination of plasma 25-hydroxyvitamin D [25(OH)D] concentrations. Lifestyle information was obtained from 8498 participants, with some missing values regarding different lifestyle factors. Multiple logistic regression analysis was used to obtain odds ratios for vitamin D sufficiency, which was defined as a plasma 25(OH)D concentration &gt;= 75 nmol/L.
    Results: The prevalence of vitamin D sufficiency (i.e., plasma 25(OH)D concentration &gt;= 75 nmol/L) was 9.1%, and significant associations were observed with male gender (P &lt;0.0001; OR = 2.37, 95% CI: 1.84-3.05), older age (P for trend &lt;0.0001), lower BMI (P for trend &lt;0.0001), higher METs score (P for trend = 0.0138), higher vitamin D intake (P for trend = 0.0467), summer season (P for trend &lt;0.0001), longer duration outdoors (P for trend = 0.0026), no sunscreen use (P = 0.0135; OR = 1.40, 95% CI: 1.07-1.82), higher salmon consumption (P for trend &lt;0.0001), higher alcohol consumption (P for trend &lt;0.0001), and lower coffee consumption (P for trend = 0.0025). Unlike other populations previously reported, vitamin D sufficiency was associated with older age.
    Conclusions: The prevalence of vitamin D sufficiency (i.e., 25[OH]D &gt;= 75 nmol/L) was low (9.1%) in this Japanese population. A number of demographic, environmental, and lifestyle factors are associated with vitamin D sufficiency, and thus lifestyle modification may present an opportunity to achieve vitamin D sufficiency. (c) 2015 Elsevier Inc. All rights reserved.

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  • Chondroitin sulphate N-acetylgalactosaminyl-transferase-1 inhibits recovery from neural injury 査読

    Kosei Takeuchi, Nozomu Yoshioka, Susumu Higa Onaga, Yumi Watanabe, Shinji Miyata, Yoshino Wada, Chika Kudo, Masayasu Okada, Kentaro Ohko, Kanako Oda, Toshiya Sato, Minesuke Yokoyama, Natsuki Matsushita, Masaya Nakamura, Hideyuki Okano, Kenji Sakimura, Hitoshi Kawano, Hiroshi Kitagawa, Michihiro Igarashi

    NATURE COMMUNICATIONS   4   2740   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Extracellular factors that inhibit axon growth and intrinsic factors that promote it affect neural regeneration. Therapies targeting any single gene have not yet simultaneously optimized both types of factors. Chondroitin sulphate (CS), a glycosaminoglycan, is the most abundant extracellular inhibitor of axon growth. Here we show that mice carrying a gene knockout for CS N-acetylgalactosaminyltransferase-1 (T1), a key enzyme in CS biosynthesis, recover more completely from spinal cord injury than wild-type mice and even chondroitinase ABC-treated mice. Notably, synthesis of heparan sulphate (HS), a glycosaminoglycan promoting axonal growth, is also upregulated in TI knockout mice because HS-synthesis enzymes are induced in the mutant neurons. Moreover, chondroitinase ABC treatment never induces HS upregulation. Taken together, our results indicate that regulation of a single gene, T1, mediates excellent recovery from spinal cord injury by optimizing counteracting effectors of axon regeneration-an extracellular inhibitor of CS and intrinsic promoters, namely, HS-synthesis enzymes.

    DOI: 10.1038/ncomms3740

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  • Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development 査読

    Yumi Watanabe, Kosei Takeuchi, Susumu Higa Onaga, Michiko Sato, Mika Tsujita, Manabu Abe, Rie Natsume, Minqi Li, Tatsuya Furuichi, Mika Saeki, Tomomi Izumikawa, Ayumi Hasegawa, Minesuke Yokoyama, Shiro Ikegawa, Kenji Sakimura, Norio Amizuka, Hiroshi Kitagawa, Michihiro Igarashi

    BIOCHEMICAL JOURNAL   432 ( 1 )   47 - 55   2010年11月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PORTLAND PRESS LTD  

    CS (chondroitin sulfate) is a glycosaminoglycan species that is widely distributed in the extracellular matrix. To understand the physiological roles of enzymes involved in CS synthesis, we produced CSGalNAcT1 (CS N-acetylgalactosaminyltransferase 1)-null mice. CS production was reduced by approximately half in CSGalNAcT1-null mice, and the amount of short-chain CS was also reduced. Moreover, the cartilage of the null mice was significantly smaller than that of wild-type mice. Additionally, type-II collagen fibres in developing cartilage were abnormally aggregated and disarranged in the homozygous mutant mice. These results suggest that CSGalNAcT1 is required for normal CS production in developing cartilage.

    DOI: 10.1042/BJ20100847

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  • Transplantation of cultured choroid plexus epithelial cells via cerebrospinal fluid shows prominent neuroprotective effects against acute ischemic brain injury in the rat 査読

    Naoya Matsumoto, Akihiko Taguchi, Hitoshi Kitayama, Yumi Watanabe, Masayoshi Ohta, Tomoyuki Yoshihara, Yutaka Itokazu, Mari Dezawa, Yoshihisa Suzuki, Hisashi Sugimoto, Makoto Noda, Chizuka Idel

    NEUROSCIENCE LETTERS   469 ( 3 )   283 - 288   2010年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Choroid plexus (CP) epithelial cells (CPECs) produce cerebrospinal fluid (CSF) to provide the CNS with a specialized microenvironment. Our previous study showed that the conditioned medium of cultured CPECs enhanced the survival and neurite extension of hippocampal neurons. The present study examined the ability of cultured CPECs to protect against ischemic brain injury when transplanted into the CSF. Rats were subjected to a transient occlusion of the middle cerebral artery, followed by an injection of cultured CPECs into the fourth ventricle. The injection markedly reduced neurological deficits and infarction volume within 24 h. Other beneficial effects were (1) a reduction in number of apoptotic and inflammatory cells, (2) an up-regulation of the mRNA expression of an anti-apoptotic effecter, cAMP-response element binding protein, and (3) a down-regulation of the production of pro-inflammatory factors such its interleukin-1 beta and inducible nitric oxide synthase. The injected CPECs were located within the ventricles and on the brain&apos;s surface, not in the ischemic foci, suggesting that they exert their effects by releasing diffusible neuroprotective factors into the CSF. The transplantation of CPECs via CSF is a potential new strategy for protecting against ischemic brain injury. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2009.09.060

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  • Neuroprotective effect of bone marrow-derived mononuclear cells promoting functional recovery from spinal cord injury 査読

    Tomoyuki Yoshihara, Masayoshi Ohta, Yutaka Itokazu, Naoya Matsumoto, Mari Dezawa, Yoshihisa Suzuki, Akihiko Taguchi, Yumi Watanabe, Yasushi Adachi, Susumu Ikehara, Hisashi Sugimoto, Chizuka Ide

    JOURNAL OF NEUROTRAUMA   24 ( 6 )   1026 - 1036   2007年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MARY ANN LIEBERT INC  

    Neural cell transplantation, a new therapeutic strategy for replacing injured neural components and obtaining functional recovery, has shown beneficial effects in animal models. Use of this strategy in human patients, however, requires that a number of serious issues be addressed, including ethics, immunorejection, and the therapeutic time window within which the procedure will be effective. Bone marrow-derived mononuclear cells (BM-MNC) are attractive for transplantation because they can be used as an autograft, can be easily collected within a short time period, and do not have to be cultured. In a rat model of spinal cord injury (SCI), we transplanted BM-MNC at 1 h after SCI at Th 8-9 by injecting them into the cerebrospinal fluid (CSF), and investigated the effect of this on neurologic function. In the acute stage of injury, we found a neuroprotective antiapoptotic effect, with an elevated concentration of hepatocyte growth factor in CSF. At I week after transplantation, the Basso-Beattie-Bresnahan locomotor score had increased significantly over its baseline value. In the chronic stage of injury, we observed suppressed cavity formation and functional improvement. We conclude that transplantation of BM-MNC after SCI has a remarkable neuroprotective effect in the acute stage of injury, suppressing cavity formation, and contributing to functional recovery. Our results suggest that transplantation of BM-MNC via the CSF is a potentially effective means of enhancing functional recovery after SCI in humans.

    DOI: 10.1089/neu.2007.132R

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  • Conditioned medium of the primary culture of rat choroid plexus epithelial (modified ependymal) cells enhances neurite outgrowth and survival of hippocampal neurons 査読

    Y Watanabe, N Matsumoto, M Dezawa, Y Itokazu, T Yoshihara, C Ide

    NEUROSCIENCE LETTERS   379 ( 3 )   158 - 163   2005年5月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    The choroid plexus epithelial (modified ependymal) cells (CPECs) are specialized for cerebrospinal fluid (CSF) production and serve as blood-CSF barrier. It is suggested that, in addition to CSF production, the CPECs may regulate CNS function through expression of secretory factors into CSF. There have been reports that the CPECs express various types of factors including growth factors. However, the actual effects of the molecules produced and secreted from the CPECs on the central nervous system (CNS) are virtually unknown both in vivo and in vitro. With the use of pure culture of CPECs, we demonstrated that the conditioned medium (CM) from CPECs can enhance neurite outgrowth and survival of cultured neurons derived from rat hippocampus on postnatal day 1 in 24-h cultures. The effect of the CM was retained in fractions that contains complex of molecules larger than 50 kDa in native condition with ultrafiltration method and disappeared by trypsin digestion. The results of the present study indicate that CPECs can support the survival and function of neurons in vitro by secreting factors that are likely to be of peptide/protein nature rather than small chemicals. &COPY; 2004 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2004.12.068

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  • Conversion of myoblasts to physiologically active neuronal phenotype 査読

    Y Watanabe, S Kameoka', Gopalakrishnan, V, KD Aldape, ZZZ Pan, FF Lang, S Majumder

    GENES & DEVELOPMENT   18 ( 8 )   889 - 900   2004年4月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT  

    Repressor element 1 (RE1)-silencing transcription factor (REST)/neuron-restrictive silencer factor (NRSF) can repress several terminal neuronal differentiation genes by binding to a specific DNA sequence (RE1/ neuron-restrictive silencer element [NRSE]) present in their regulatory regions. REST-VP16 binds to the same RE1/NRSE, but activates these REST/NRSF target genes. However, it is unclear whether REST-VP16 expression is sufficient to cause formation of functional neurons either from neural stem cells or from heterologous stem cells. Here we show that the expression of REST-VP16 in myoblasts grown under muscle differentiation conditions blocked entry into the muscle differentiation pathway, countered endogenous REST/NRSF-dependent repression, activated the REST/NRSF target genes, and, surprisingly, activated other neuronal differentiation genes and converted the myoblasts to a physiologically active neuronal phenotype. Furthermore, in vitro differentiated neurons produced by REST-VP16-expressing myoblasts, when injected into mouse brain, survived, incorporated into the normal brain, and did not form tumors. This is the first instance in which myoblasts were converted to a neuronal phenotype. Our results suggest that direct activation of REST/NRSF target genes with a single transgene, REST-VP16, is sufficient to activate other terminal neuronal differentiation genes and to override the muscle differentiation pathways, and they suggest that this approach provides an efficient way of triggering neuronal differentiation in myoblasts and possibly other stem cells.

    DOI: 10.1101/gad.1179004

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  • Appearance of the LAT protein at an early stage of B-cell development and its possible role 査読

    K Oya, JY Wang, Y Watanabe, R Koga, T Watanabe

    IMMUNOLOGY   109 ( 3 )   351 - 359   2003年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING LTD  

    The linker protein LAT is expressed mainly in T and natural killer (NK) cells. LAT-deficient mice have an arrest of intrathymic T-cell development at the CD4(+) CD8(+) stage and lack mature T cells in the periphery. However, no gross abnormality in expressed in mouse progenitor B (pro-B) and precursor B (pre-B) cells, but not in immature or mature B cells. LAT in pre-B cells becomes tyrosine phosphorylated upon cross-linking of the pre-B-cell receptor (pre-BCR) by anti-mu anti-body. Incubation of 1xN/2b (mouse pre-B-cell line) cells or bone marrow cells from muMT/muMT mice, which lack B cells after the small pre-B-cell stage, with anti-Igbeta antibody resulted in the downregulation of LAT expression. Transgenic mice which expressed LAT protein in B-lineage cells showed an increased proportion of pro- and large pre-B cells in the bone marrow and a remarkable reduction in the numbers of mature B cells in peripheral lymphoid tissues. Collectively, the present results indicate that LAT is expressed in the cells at the early stages of B-lineage development, but is absent in immature and mature B cells. LAT may play a crucial role in the negative regulation of B-cell development at the transition from pre-B to mature B-cell stages, and signal(s) via the pre-BCR may extinguish LAT expression, thus allowing pre-B-cell differentiation towards the mature B-cell stage.

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  • pRb phosphorylation is regulated differentially by cyclin-dependent kinase (Cdk) 2 and Cdk4 in retinoic acid-induced neuronal differentiation of P19 cells 査読

    Y Watanabe, T Watanabe, M Kitagawa, Y Taya, K Nakayama, N Motoyama

    BRAIN RESEARCH   842 ( 2 )   342 - 350   1999年9月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The retinoblastoma protein (pRb) is a key regulator of cell growth, differentiation and survival. pRb(-/-) mice show abnormal neuronal cell death in the developing brain. The function of pRb is regulated by its phosphorylation state. In this study, the phosphorylation of pRb during retinoic acid (RA)-induced neuronal differentiation of P19 cells was examined using site-specific antibodies against pRb phosphorylated at Ser601, Ser605 and Ser773. Although pRb was hyperphosphorylated in undifferentiated P19 cells, Ser601 and Ser773 were not phosphorylated. Upon exposure to RA, however, these two sites became strongly phosphorylated, Cdk4 kinase activity was almost undetectable in undifferentiated P19 cells, but was strongly activated on exposure to RA. In contrast, Cdk2 kinase activity and the phosphorylation of Ser605 were observed in undifferentiated cells as well as in RA-treated cells. These observations suggest that Cdk2 and Cdk4 may phosphorylate different sites of pRb in vivo and that the two sites of pRb examined here are newly phosphorylated during RA-induced neuronal differentiation in P19 cells. (C) 1999 Elsevier Science B.V. All rights reserved.

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MISC

  • 地域在住高齢者の認知機能低下予防プログラムの開発

    北村 香織, 佐藤 久美, 林 直美, 渡邊 裕美, 中村 和利

    木村看護教育振興財団看護研究集録   ( 27 )   36 - 60   2020年11月

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    記述言語:日本語   出版者・発行元:(公財)木村看護教育振興財団  

    地域高齢者の認知機能低下に関わる要因を明らかにするため、小千谷市の3地区に在住する高齢者で要支援・要介護認定を受けていない592名に面接調査を行い、その結果をもとに、科学的根拠に基づく認知機能低下予防プログラムを開発した。同プログラムを用いて認知機能低下予防教室を開催し、そのさい教育媒体として「生活習慣チェック用紙」を使用するとともに、面談時に「K10調査票」を用いてストレスの評価を行うこととした。さらに、教室終了後に面談結果をスタッフ間で検討することでハイリスク者を抽出し、個別支援を継続的に実施することとした。

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  • 認知症バイオマーカー探索の新潮流 尿プロテオミクスによる認知症バイオマーカー探索

    渡邊 裕美

    Dementia Japan   33 ( 4 )   500 - 500   2019年10月

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    記述言語:日本語   出版者・発行元:(一社)日本認知症学会  

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  • 尿プロテオミクスによるアルツハイマー病バイオマーカー研究

    渡邊 裕美

    BIO Clinica   34 ( 9 )   936 - 940   2019年8月

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    記述言語:日本語   出版者・発行元:(株)北隆館  

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  • 尿プロテオミクスによるアルツハイマー病早期診断マーカーの開発

    渡邊 裕美

    先進医薬研究振興財団研究成果報告集   2018年度   52 - 53   2019年3月

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    記述言語:日本語   出版者・発行元:(公財)先進医薬研究振興財団  

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  • 非標識プロテオミクスによる尿を用いたアルツハイマー病バイオマーカーの可能性

    渡邊 裕美, 平尾 嘉利, 春日 健作, 徳武 孝允, 北村 香織, 池内 健, 山本 格, 中村 和利

    日本衛生学雑誌   74 ( Suppl. )   S147 - S147   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本衛生学会  

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  • 新潟大学のコホート研究・臨床疫学研究の進捗状況と今後の展望 村上コホート研究

    中村 和利, 北村 香織, 渡邊 裕美

    新潟医学会雑誌   132 ( 4 )   127 - 130   2018年4月

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    記述言語:日本語   出版者・発行元:新潟医学会  

    私たちは、健康寿命延伸とビタミンDの加齢性疾患予防効果の解明を目的として、加齢性運動器疾患、認知症、その他の疾患のリスク要因や予防要因を包括的に解明する地域住民コホート研究を2011年に新潟県村上保健所管内で開始した。エンドポイントは、死亡と疾患罹患率であり、対象疾患は、骨粗鬆症性骨折、変形性膝関節症、認知症、慢性疼痛、その他である。健康調査票によるベースライン調査に同意した参加者(n=14,364)の平均年齢は、男性59.2歳(SD=9.3、n=6,907)、女性59.0歳(SD=9.3、n=7,457)であった。また、血液検体のある参加者(n=8,497)の平均年齢は、男性56.5歳(SD=18.4、n=3,710)、女性45.4歳(SD=16.5、n=4,787)であった。調査票による5年後フォローアップ調査を2016〜2017年に行い(回収率61%)、疾患追跡を順調に進めている。(著者抄録)

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  • 尿プロテオミクスによるアルツハイマー病バイオマーカー探索

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    Dementia Japan   31 ( 4 )   611 - 611   2017年10月

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  • 地域総合病院通院患者における透析療法と認知機能スケールとの関連 PROST

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  • 地域総合病院通院患者における透析療法と認知機能スケールとの関連 PROST

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    Dementia Japan   30 ( 4 )   550 - 550   2016年10月

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    記述言語:日本語   出版者・発行元:(一社)日本認知症学会  

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  • 地域在住中高年者の認知機能と教育歴の関連

    北村 香織, 渡邊 裕美, 中村 和利

    日本老年医学会雑誌   53 ( Suppl. )   151 - 151   2016年5月

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  • 地域総合病院通院患者の血清高感度C反応性蛋白(CRP)と認知機能スケールとの関連 PROST

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    Dementia Japan   29 ( 3 )   368 - 368   2015年9月

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  • THE MURAKAMI COHORT STUDY FOR THE PREVENTION OF MUSCULOSKELETAL DISEASES WITH VITAMIN D IN JAPAN

    K. Nakamura, K. Kitamura, R. Takachi, T. Saito, R. Kobayashi, R. Oshiki, Y. Watanabe

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  • 前シナプス可塑性の変化に関する,変異シンタキシン1A(R151G)ノックインマウスと,カルシウム/カルモジュリン依存性プロテインキナーゼII(CaMKII)改変マウスの類似性

    渡邊裕美, 片山憲和, 武内恒成, 高雄啓三, 宮川剛, 崎村建司, 真鍋俊也, 五十嵐道弘

    日本分子生物学会年会プログラム・要旨集(Web)   36th   WEB ONLY 3P-0709   2013年

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    J-GLOBAL

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  • Abnormality in the SNARE-related complex in the syntaxin-1A (R151G) knock-in mice

    Yumi Watanabe, Michitoshi Watanabe, Kenji Sakimura, Michihiro Igarashi

    NEUROSCIENCE RESEARCH   71   E320 - E320   2011年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER IRELAND LTD  

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  • Brain development abnormality in the mice lacking in the enzymes synthesizing chondroitin sulfate

    Susumu Higa Onaga, Kosei Takeuchi, Yumi Watanabe, Yukari Komuta, Tomomi Izumikawa, Hitoshi Kitagawa, Michihiro Igarashi

    NEUROSCIENCE RESEARCH   68   E250 - E250   2010年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2010.07.1108

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  • Syntaxin-1A (R151G) knock-in mice that cannot be associated with CaMKII show the impaired dynamics of syntaxin-1A and vesicle recycling after stimulation

    Yumi Watanabe, Yukiyo Tada, Kenji Sakimura, Michihiro Igarashi

    NEUROSCIENCE RESEARCH   68   E232 - E232   2010年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2010.07.1024

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  • Functional analyses of mice lacking an enzyme for chondroitin sulfate synthesis

    Susumu Higa Onaga, Yumi Watanabe, Kosei Takeuchi, Mitihiro Igarashi

    NEUROSCIENCE RESEARCH   65   S163 - S163   2009年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2009.09.842

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  • Analysis of the synaptic vesicle transportation of Syntaxin-1A (R151G) knock-in mouse

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    NEUROSCIENCE RESEARCH   65   S141 - S141   2009年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2009.09.705

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  • 【細胞シグナル伝達分子に関する最近の進歩】 免疫細胞活性化に関与するアダプター分子

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    臨床免疫   34 ( 5 )   617 - 626   2000年11月

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  • 尿プロテオミクスによる認知症バイオマーカー探索

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(指名)  

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  • アルツハイマー病患者尿中タンパク質の分子ネットワーク解析

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  • 尿プロテオミクスによるアルツハイマー病バイオマーカー探索

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    第36回日本認知症学会学術集会(ポスター)  2017年11月 

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  • アルツハイマー病患者の尿中蛋白質プロファイルの解析

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    日本プロテオーム学会2017年大会(JHUPO第15回大会) (ポスター)  2017年7月 

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  • Elevated C-reactive protein is associated with cognitive decline in outpatients of a general hospital: The Project in Sado for Total Health (PROST). 国際会議

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    The 7th BRI International Symposium 2017 (ポスター)  2017年3月 

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  • 地域総合病院通院患者における透析療法と認知機能スケールとの関連:PROST

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  • 地域総合病院通院患者の血清高感度C反応性蛋白(CRP)と認知機能スケールとの関連:PROST

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    第34回日本認知症学会学術集会 (ポスター)  2015年10月 

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  • 前シナプス可塑性の変化に関する,変異シンタキシン1A(R151G)ノックインマウスと,カルシウム/カルモジュリン依存性プロテインキナーゼII(CaMKII)改変マウスの類似性

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    第36回日本分子生物学会年会 (ポスター)  2013年12月 

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  • 短時間の午睡は高齢者の認知機能低下を予防する:5年間の縦断的研究

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  • Modifiable factors associated with cognitive impairment in 1143 Japanese outpatients: The Project in Sado for Total Health (PROST). 国際会議

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    The 7th BRI International Symposium 2017 (ポスター)  2017年3月 

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  • 地域在住中高年者の認知機能と教育歴の関連

    北村香織, 渡邊裕美, 中村和利

    第58回日本老年医学会学術集会 (ポスター)  2016年6月 

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  • シナプス小胞エキソサイトーシスへのカルシウム/カルモジュリン依存性プロテインキナーゼII(CaMKII)の関与;変異Syntaxin‐1A(R151G)の解析

    佐藤駿平, 渡邊裕美, 武内恒成, 五十嵐道弘

    第36回日本分子生物学会年会 (ポスター)  2013年12月 

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  • Presynaptic CaMKII regulates sbort・term plasticity via syntaxin.

    ○Michihiro Igarashi, Yumi Watanabe, Hirokazu Katayama, Toshiya Manabe, Keizo Takao, Tsuyoshi Miyakawa

    The 22nd IBNS Annual Meeting 2013, Poster #116.  2013年1月 

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  • シンタキシン1A(R151G)KIマウスにおけるSNARE複合体構成の変化(Altered protein complex for exocytosis observed in the syntaxin-1A (R151G) knock-in mice)

    渡邊 裕美, 武内 恒成, 崎村 建司, 五十嵐 道弘

    第85回日本生化学会大会 (ポスター)  2012年12月 

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  • コンドロイチン硫酸の発現制御による神経再生機構

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    第85回日本生化学会大会 (ポスター)  2012年12月 

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    記述言語:日本語  

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  • プロテオグライカン合成系の調整による神経損傷修復機構

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    第35回日本神経科学大会 O3-G-47-2  2012年9月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • 変異シンタキシン1A-KI マウス神経細胞における開口放出異常の分子基盤の解析

    渡邊裕美, 渡部通寿, 崎村建司, 五十嵐道弘

    第34回日本神経科学大会  2011年9月 

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    会議種別:ポスター発表  

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  • 変異シンタキシン1Aノックインマウス由来海馬神経細胞におけるシナプス開口放出機構の解析(Syntaxin-1A (R151G) knock-in mice that cannot be associated with CaMKII show the impaired dynamics of syntaxin-1A and vesicle recycling after stimulation)

    渡邊 裕美, 多田 幸代, 崎村 建司, 五十嵐 道弘

    Neuro2010 (第33回日本神経科学大会、第53回日本神経化学大会、第20回日本神経回路学会大会 合同大会) (ポスター)  2010年9月 

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  • コンドロイチン硫酸合成酵素欠損マウスにおける脳発達異常

    ○Higa Onaga Susumu, 武内 恒成, 渡邊 裕美, 小牟田 縁, 泉川 友美, 北川 裕之, 五十嵐 道弘

    Neuro2010 (第33回日本神経科学大会、第53回日本神経化学大会、第20回日本神経回路学会大会 合同大会) (ポスター)  2010年8月 

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  • Possible involvement of syntaxin1A-CaMKII interaction in the regulation of exocytosis in mouse chromaffin cells

    Kyo Hirotaka, Hayashi Mitsunori, Sasakawa Nobuyuki, Watanabe Yumi, Igarashi Michihiro, Sakimura Kenji, Kumakura Konosuke

    第83回日本薬理学会年会  2010年 

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    会議種別:ポスター発表  

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  • CaMKII結合部位に変異を有するシンタキシン‐1Aを導入したノックインマウスにおけるSNARE複合体形成の解析

    渡邊裕美, 渡部通寿, 多田幸代, 番場彩子, 五十嵐道弘

    生化学  2009年9月 

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  • コンドロイチン硫酸プロテオグリカンの糖鎖生合成酵素欠損マウスの解析(Analyses of the knockout mice lacking in the enzymes synthesizing chondroitin sulfate)

    比嘉 進, 渡邊 裕美, 武内 恒成, 五十嵐 道弘

    神経化学  2009年6月 

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  • シナプス小胞輸送とマウス行動におけるシンタキシン-1A-CaMKII相互作用の意義(The significance of Syntaxin-1A-CaMKII interaction in synaptic vesicle transportation and mouse behavior)

    渡邊 裕美, 伊藤 理恵子, 多田 幸代, 高雄 啓三, 宮川 剛, 崎村 建司, 五十嵐 道弘

    神経化学  2009年6月 

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    記述言語:英語  

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  • Analysis of the synaptic vesicle transportation of Syntaxin-1A (R151G) knock-in mouse

    Watanabe Yumi, Tada Yukiyo, Banba Ayako, Sakimura Kenji, Igarashi Michihiro

    NEUROSCIENCE RESEARCH  2009年 

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  • マウスクロマフィン細胞における開口分泌の特性と分泌小胞の動態(Characterization of exocytotic events and the dynamics of secretory vesicles in single mouse chromaffin cells)

    笹川 展幸, 松田 冬香, 林 光紀, 渡邊 裕美, 五十嵐 道弘, 崎村 建司, 熊倉 鴻之助

    神経化学  2008年8月 

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    記述言語:英語  

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  • 自己リン酸化CaMKII結合能を有しない変異シンタキシン1Aノックインマウスにおけるシナプス小胞輸送の変化

    渡邊裕美, 多田幸代, 伊藤理恵子, 五十嵐道弘

    生化学  2008年 

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    記述言語:日本語  

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  • 自己リン酸化型CaMKII結合能を阻害する変異を導入したSyntaxin‐1A knock‐inマウスの作成と解析

    渡邊裕美, 伊藤理恵子, 大湖健太郎, 崎村建司, 宮川剛, 五十嵐道弘

    生化学  2007年 

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    記述言語:日本語  

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  • 脈絡叢上衣細胞初代培養の培養上清はラット海馬由来神経細胞の突起伸長と生存を促進する

    渡邊裕美, 松本直也, 出澤真理, 糸数裕, 吉原智之, 井出千束

    日本分子生物学会 第5回 春季シンポジウム  2005年5月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • 脈絡叢上衣細胞初代培養の培養上清はラット海馬由来神経細胞の突起伸長と生存を促進する

    渡邊 裕美, 松本 直也, 出澤 真理, 糸数 裕, 吉原 智之, 井出 千束

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集  2004年10月 

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    記述言語:日本語  

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  • Conditioned medium of the primary cultured rat choroid plexus ependymal cellsenhances neurite outgrowth and survival of neurons derived from hippocampus

    ○Yumi Watanabe, Naoya Matsumoto, Mari Dezawa, Yutaka Itokazu, Tomoyuki Yoshihara, Chizuka Ide

    16th International Congress of International Federation of Association of Anatomists/第109回日本解剖学会総会共催、P4-181  2004年8月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • プロB細胞,プレB細胞でのLATアダプター分子の発現とB細胞分化チェックポイントにおける機能

    大屋 和之, 渡邊 裕美, 古賀 律子, 渡邊 武

    日本免疫学会総会・学術集会記録  2000年11月 

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    記述言語:日本語  

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  • 神経分化時におけるRb蛋白のリン酸化と細胞周期制御機構の解析

    渡邊裕美, 渡邊武, 北川雅敏, 中山敬一, 本山昇

    第71回日本生化学会大会 ワークショップ3H-W23-04  1998年10月 

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  • 神経細胞の分化におけるRbの機能制御メカニズムの解析

    本山 昇, 渡邊 裕美, 北川 雅敏, 中山 敬一, 渡邊 武

    神経化学  1998年9月 

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    記述言語:日本語  

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  • 細胞分化 神経分化時におけるRb蛋白のリン酸化と細胞周期制御機構の解析

    渡邊 裕美, 北川 雅敏, 中山 敬一, 渡邊 武, 本山 昇

    生化学  1998年8月 

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    記述言語:日本語  

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共同研究・競争的資金等の研究

  • 縦断研究による、尿プロテオミクスによる認知症予測バイオマーカー探索

    研究課題/領域番号:20K10427  2020年4月 - 2023年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    渡邊 裕美

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    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

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  • 尿リピドミクス解析による認知症予測バイオマーカーの探索

    研究課題/領域番号:19K21581  2019年6月 - 2021年3月

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    中村 和利, 渡邊 裕美

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    配分額:6500000円 ( 直接経費:5000000円 、 間接経費:1500000円 )

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  • 加齢性運動器疾患の大規模分子疫学コホート研究10年後フォローアップ

    研究課題/領域番号:19H03897  2019年4月 - 2023年3月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    中村 和利, 伊木 雅之, 渡邊 裕美, 渡邊 慶

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    配分額:17290000円 ( 直接経費:13300000円 、 間接経費:3990000円 )

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  • 尿プロテオミクスによるアルツハイマー病早期診断マーカーの開発

    2017年12月 - 2018年11月

    公益財団法人 先進医薬研究振興財団  精神薬療分野 一般研究助成 

    渡邊裕美

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    担当区分:研究代表者  資金種別:競争的資金

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  • 尿メタボロミクス解析による認知症予測バイオマーカーの網羅的探索

    研究課題/領域番号:17K19799  2017年6月 - 2019年3月

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    中村 和利, 渡邊 裕美, 池内 健, 山本 格, 蒲澤 佳子, 北村 香織

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    配分額:6370000円 ( 直接経費:4900000円 、 間接経費:1470000円 )

    本研究は、網羅的な低分子代謝産物の解析(メタボロミクス解析)により、アルツハイマー病(AD)患者に特有の尿中(水溶性)低分子バイオマーカーを同定することを目的とし、AD病患者群9例と対照群9例の尿検体に見出されるイオン性代謝産物の特性を予測モデルと妥当性モデルの両方で比較した。予測モデルで増加、減少した代謝産物がそれぞれ2個、7個、妥当性モデルで有意に増加、減少した代謝産物がそれぞれ3個、6個同定された。両モデルに共通して有意な減少を示した代謝産物はGlycerol-3-phosphateであった。Glycerol-3-phosphateは新たなADのバイオマーカーである可能性が示唆された。

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  • 尿メタボロミクス解析による認知症予測バイオマーカーの網羅的探索

    2017年4月 - 2019年3月

    日本学術振興会  挑戦的研究(萌芽) 

    中村和利

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    資金種別:競争的資金

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  • 尿プロテオミクス解析による認知症予測バイオマーカーの網羅的探索

    研究課題/領域番号:16K09051  2016年4月 - 2020年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    渡邊 裕美, 山本 格, 中村 和利

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    配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

    認知症患者、中でもアルツハイマー病(AD)患者の増加は著しく、ハイリスク群への予防的介入のための簡便なバイオマーカー開発がのぞまれる。尿は非侵襲的に採取できる生体試料であり、プロテオミクス解析にも適した素材である。本研究の目的は尿を用いた ADの早期診断、予測バイオマーカーの探索を行うことである。
    これまでに、AD患者尿、認知機能正常対照尿18検体に対し非標識LC-MS/MS解析を行い、normalized spectral index法 (SIN)により半定量を行いプロテオミクスデータを得た。プロテオミクスで有意に変動したタンパク質についてELISAを行ったところ複数のタンパク質が質量分析結果同様に有意な変動を示すことを見いだした。
    H30年度は以下を実施した。①得られたプロテオミクスデータから分子ネットワーク解析と遺伝子オントロジーエンリッチメント解析を行った。その結果、アルツハイマー病患者尿におけるタンパク質群の変化はリソソーム、糖代謝、補体活性、リポタンパク質代謝、HSP90シグナリング等の変化を示していることが示唆された。この結果について論文化しDementia and cognitive disorder extra誌に投稿発表した。②妥当性解析を行うための新規患者尿、対照尿検体を国立長寿医療研究センターバイオバンクから得、候補タンパク質のELISAによる測定を開始した。③村上コホート尿検体を用いた候補タンパク質のELISA解析を開始した。

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  • 尿プロテオミクス解析による認知症予測バイオマーカーの網羅的探索

    2016年4月 - 2019年3月

    日本学術振興会  科学研究費助成事業(基盤C)  基盤研究(C)

    渡邊裕美

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    担当区分:研究代表者  資金種別:競争的資金

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  • 加齢性運動器疾患の大規模分子疫学コホート研究5年後フォローアップ

    研究課題/領域番号:15H04782  2015年4月 - 2019年3月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    中村 和利, 伊木 雅之, 渡邊 裕美, 北村 香織, 高地 リベカ, 小林 量作

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    資金種別:競争的資金

    配分額:15860000円 ( 直接経費:12200000円 、 間接経費:3660000円 )

    本研究により、2011年に開始した加齢性運動器疾患の大規模コホート研究の5年後フォローアップを完了した。具体的には、40から74歳の13,816人を対象として生活習慣等のアンケート調査を行い、8,487(61.4%)より回答を得た。また、追跡期間中に死亡373人、骨粗鬆症性骨折284人、変形性膝関節症429人が観察された。これらの資料より、死亡、骨粗鬆症性骨折、変形性膝関節症、慢性疼痛のリスク要因を解析することができた。また、5年間の生活習慣等の情報を比較することにより、ベースラインにおける曝露情報の再評価を行うことができた。

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  • シナプス開口放出を担うシンタキシン1A、1B分子の機能の違いとその原因の探索

    2011年4月 - 2014年3月

    日本学術振興会  科学研究費助成事業 (RPD)  特別研究員奨励費

    渡邊裕美

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    担当区分:研究代表者  資金種別:競争的資金

    配分額:2400000円 ( 直接経費:2400000円 )

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  • シナプス開口放出を担うシンタキシン1A、1B分子の機能の違いとその原因の探索

    研究課題/領域番号:11J40157  2011年 - 2013年

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    渡邊 裕美

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    配分額:2400000円 ( 直接経費:2400000円 )

    申請者の所属する研究室で、神経組織においてシナプス開口放出を担うSNARE複合体の一員であるシンタキシン1AがCaMKIIとCa^<2+>依存的に結合することを見出した。この結合の生理的意義を明らかにする目的で、本研究課題ではシンタキシン1Aと自己リン酸化CaMKIIとの結合を阻害する変異(R151G)を導入した変異シンタキシン1A (R151G) KIマウスの解析を行った。本マウスではシナプス開口放出やエンドサイトーシスなどに関連する蛋白質の発現量や、SDS-resistant complexの量に変化はなく、また、海馬スライスにおける基本的な神経伝達は保たれていた。一方で、KIマウスでは、SNARE複合体に結合し開口放出の細胞内Ca^<2+>濃度上昇との同期性を高める分子であるcomplexinと、SNARE複合体との結合が低下していた。さらに、電気生理学解析によりKIマウスは基礎的な神経活動に異常は見られないが、持続刺激を与えた際に野生型と異なる短期可塑性を示すことが明らかとなった。CaMKIIは後シナプスにおける長期シナプス可塑性を制御することがよく知られているが、本研究で示されるような前シナプス可塑性への関与については殆ど明らかとなっていなかった。我々の結果は神経活動に伴い高度に自己リン酸化したCaMKIIがSNRE複合体の一員であるシンタキシン1Aと結合することで前シナプス可塑性を抑制的に制御していることを明らかにした。コンプレキシンはSNARE複合体に結合して開口放出を制御する蛋白質であり、その機能として、開口放出に対し抑制/促進の二面性を持つという特徴がある。しかし、第一義的には抑制的に働くことから、自己リン酸化CaMKIIとシンタキシン1Aの結合はSNARE複合体とコンプレキシンの結合を強める方向に働くことで、前シナプス可塑性を制御していると考えられる。

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  • 脳脈絡叢細胞が産生する新たな神経栄養因子・サイトカインの同定と遺伝子の単離

    研究課題/領域番号:96J00782  1998年

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    渡邊 裕美

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    配分額:900000円 ( 直接経費:900000円 )

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  • 脳脈絡叢が分泌する新たな神経栄養因子・サイトカインの同定と遺伝子の単離

    1996年4月 - 1999年3月

    日本学術振興会  科学研究費助成事業 (DC1)  特別研究員奨励費

    渡邊裕美

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    担当区分:研究代表者  資金種別:競争的資金

    配分額:2700000円 ( 直接経費:2700000円 )

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担当経験のある授業科目(researchmap)

  • 環境医学(講義/水・大気)

    2017年
    -
    現在
    機関名:新潟大学

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    科目区分:学部専門科目  国名:日本国

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  • 環境医学(修士課程)

    2017年
    -
    現在
    機関名:新潟大学

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    科目区分:大学院教養科目  国名:日本国

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  • 疫学(演習)

    2015年
    -
    現在
    機関名:新潟大学

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    科目区分:学部専門科目  国名:日本国

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  • 遺伝学と保健医療

    2013年
    -
    2018年
    機関名:新潟医療福祉大

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  • 肉眼解剖学実習

    2004年
    機関名:京都大学

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  • 組織学実習

    2004年
    機関名:京都大学

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担当経験のある授業科目

  • 病気と遺伝学

    2021年
    -
    現在
    機関名:新潟大学

  • 環境医学

    2017年
    -
    現在
    機関名:新潟大学

  • 疫学

    2015年
    -
    現在
    機関名:新潟大学