2024/10/07 更新

写真a

タテイシ ヨシタカ
立石 善隆
TATEISHI Yoshitaka
所属
教育研究院 医歯学系 医学系列 准教授
医歯学総合研究科 地域疾病制御医学専攻 国際感染医学 准教授
職名
准教授
外部リンク

学位

  • 博士(医学) ( 2006年3月   大阪市立大学 )

研究分野

  • ライフサイエンス / 細菌学

経歴(researchmap)

  • 新潟大学   医歯学総合研究科 地域疾病制御医学専攻 国際感染医学   准教授

    2014年5月 - 現在

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経歴

  • 新潟大学   医歯学総合研究科 地域疾病制御医学専攻 国際感染医学   准教授

    2014年5月 - 現在

 

論文

  • Mycobacterial DNA-binding protein 1 is critical for BCG survival in stressful environments and simultaneously regulates gene expression. 国際誌

    Amina K Shaban, Gebremichal Gebretsadik, Mariko Hakamata, Hayato Takihara, Erina Inouchi, Akihito Nishiyama, Yuriko Ozeki, Yoshitaka Tateishi, Yukiko Nishiuchi, Takehiro Yamaguchi, Naoya Ohara, Shujiro Okuda, Sohkichi Matsumoto

    Scientific reports   13 ( 1 )   14157 - 14157   2023年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Survival of the live attenuated Bacillus Calmette-Guérin (BCG) vaccine amidst harsh host environments is key for BCG effectiveness as it allows continuous immune response induction and protection against tuberculosis. Mycobacterial DNA binding protein 1 (MDP1), a nucleoid associated protein, is essential in BCG. However, there is limited knowledge on the extent of MDP1 gene regulation and how this influences BCG survival. Here, we demonstrate that MDP1 conditional knockdown (cKD) BCG grows slower than vector control in vitro, and dies faster upon exposure to antibiotics (bedaquiline) and oxidative stress (H2O2 and menadione). MDP1-cKD BCG also exhibited low infectivity and survival in THP-1 macrophages and mice indicating possible susceptibility to host mediated stress. Consequently, low in vivo survival resulted in reduced cytokine (IFN-gamma and TNF-alpha) production by splenocytes. Temporal transcriptome profiling showed more upregulated (81-240) than downregulated (5-175) genes in response to MDP1 suppression. Pathway analysis showed suppression of biosynthetic pathways that coincide with low in vitro growth. Notable was the deferential expression of genes involved in stress response (sigI), maintenance of DNA integrity (mutT1), REDOX balance (WhiB3), and host interactions (PE/PE_PGRS). Thus, this study shows MDP1's importance in BCG survival and highlights MDP1-dependent gene regulation suggesting its role in growth and stress adaptation.

    DOI: 10.1038/s41598-023-40941-9

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  • Virulence of Mycobacterium intracellulare clinical strains in a mouse model of lung infection - role of neutrophilic inflammation in disease severity. 国際誌

    Yoshitaka Tateishi, Yuriko Ozeki, Akihito Nishiyama, Mari Miki, Ryoji Maekura, Hiroshi Kida, Sohkichi Matsumoto

    BMC microbiology   23 ( 1 )   94 - 94   2023年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Mycobacterium intracellulare is a major etiological agent of Mycobacterium avium-intracellulare pulmonary disease (MAC-PD). However, the characteristics of the virulence of M. intracellulare and the in vivo chemotherapeutic efficacy remain unclear. In this study, we examined the virulence of nine M. intracellulare strains with different clinical phenotypes and genotypes in C57BL/6 mice. RESULTS: We classified three types of virulence phenotypes (high, intermediate, and low) based on the kinetics of the bacterial load, histological lung inflammation, and neutrophilic infiltration. High virulence strains showed more severe neutrophilic infiltration in the lungs than intermediate and low virulence strains, with 6.27-fold and 11.0-fold differences of the average percentage of neutrophils in bronchoalveolar lavage fluid, respectively. In particular, the high virulence strain M.i.198 showed the highest mortality in mice, which corresponded to the rapid progression of clinical disease. In mice infected with the drug-sensitive high virulence strain M019, clarithromycin-containing chemotherapy showed the highest efficacy. Monotherapy with rifampicin exacerbated lung inflammation with increased lymphocytic and neutrophilic infiltration into the lungs. CONCLUSIONS: The virulence phenotypes of clinical strains of M. intracellulare were diverse, with high virulence strains being associated with neutrophilic infiltration and disease progression in infected mice. These high virulence strains were proposed as a useful subject for in vivo chemotherapeutic experiments.

    DOI: 10.1186/s12866-023-02831-y

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  • Lysocin E Targeting Menaquinone in the Membrane of Mycobacterium tuberculosis Is a Promising Lead Compound for Antituberculosis Drugs. 査読 国際誌

    Geberemichal Geberetsadik, Akane Inaizumi, Akihito Nishiyama, Takehiro Yamaguchi, Hiroshi Hamamoto, Suresh Panthee, Aki Tamaru, Manabu Hayatsu, Yusuke Mizutani, Shaban Amina Kaboso, Mariko Hakamata, Aleksandr Ilinov, Yuriko Ozeki, Yoshitaka Tateishi, Kazuhisa Sekimizu, Sohkichi Matsumoto

    Antimicrobial agents and chemotherapy   66 ( 9 )   e0017122   2022年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Tuberculosis remains a public health crisis and a health security threat. There is an urgent need to develop new antituberculosis drugs with novel modes of action to cure drug-resistant tuberculosis and shorten the chemotherapy period by sterilizing tissues infected with dormant bacteria. Lysocin E is an antibiotic that showed antibacterial activity against Staphylococcus aureus by binding to its menaquinone (commonly known as vitamin K2). Unlike S. aureus, menaquinone is essential in both growing and dormant Mycobacterium tuberculosis. This study aims to evaluate the antituberculosis activities of lysocin E and decipher its mode of action. We show that lysocin E has high in vitro activity against both drug-susceptible and drug-resistant Mycobacterium tuberculosis var. tuberculosis and dormant mycobacteria. Lysocin E is likely bound to menaquinone, causing M. tuberculosis membrane disruption, inhibition of oxygen consumption, and ATP synthesis. Thus, we have concluded that the high antituberculosis activity of lysocin E is attributable to its synergistic effects of membrane disruption and respiratory inhibition. The efficacy of lysocin E against intracellular M. tuberculosis in macrophages was lower than its potent activity against M. tuberculosis in culture medium, probably due to its low ability to penetrate cells, but its efficacy in mice was still superior to that of streptomycin. Our findings indicate that lysocin E is a promising lead compound for the development of a new tuberculosis drug that cures drug-resistant and latent tuberculosis in a shorter period.

    DOI: 10.1128/aac.00171-22

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  • Direct Attachment with Erythrocytes Augments Extracellular Growth of Pathogenic Mycobacteria 査読

    Yukiko Nishiuchi, Yoshitaka Tateishi, Hiroshi Hirano, Yuriko Ozeki, Takehiro Yamaguchi, Mari Miki, Seigo Kitada, Fumito Maruyama, Sohkichi Matsumoto

    Microbiology Spectrum   10 ( 2 )   2022年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    Pathogenic mycobacteria, such as Mycobacterium tuberculosis , Mycobacterium avium subsp. hominissuis (MAH), and Mycobacterium intracellulare , cause pulmonary infections as intracellular parasites of lung macrophages and epithelial cells. Here, using histopathological examinations we found that MAH and M. intracellulare colocalized with erythrocytes in lung infection sites.

    DOI: 10.1128/spectrum.02454-21

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  • Long-Term Prognosis and Antimycobacterial Glycolipid Antibody as Biomarker in Mycobacterium avium-intracellulare Complex Pulmonary Disease. 国際誌

    Ryoji Maekura, Keisuke Miki, Yoshitaka Tateishi, Sohkichi Matsumoto, Seigo Kitada, Mari Miki, Hiroshi Kida

    Microbiology spectrum   e0053022   2022年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Clinical characteristics and outcomes of multidrug chemotherapy have been used as the main prognostic factors for Mycobacterium avium-intracellulare complex pulmonary disease (MAC-PD) over the last decade; however, no useful prognostic biomarkers have been reported. The aim is to ascertain whether the serum antibody titers could include useful prognostic predictors of MAC-PD. Ninety-four patients with MAC-PD were enrolled and regularly followed up with for more than 5 years or until death. Cox proportional hazard regression and receiver operating characteristic (ROC) curve analyses were used to identify predictors of mortality in this prospective observational study. According to treatment outcomes, 85 patients completed follow-up and were classified into four groups. Seventeen patients (20%) died during follow-up (median, 10.1 years; interquartile range, 8.1 to 12.4 years). All 11 patients with MAC-PD-specific death were included in the 14 patients of the group nonresponsive to the multidrug chemotherapy. They had significantly higher anti-Mycobacterium glycolipid (MBGL) antibody titers than those in the other groups and a significantly (P < 0.0001) poorer survival prognosis. The anti-MBGL antibody titers also served as a negative prognostic factor. A cutoff score of 7, which was calculated by clinical poor prognostic characteristics and anti-MBGL antibody titers, differentiated the nonresponse group and the other groups at baseline (sensitivity, specificity, and area under the curve: 92.9%, 81.7%, and 0.95, respectively). In conclusion, anti-MBGL antibody titers were useful to assess the refractory MAC-PD. The predictions of treatment outcome and mortality become more accurate by using anti-MBGL antibody and clinical poor prognostic characteristics together. IMPORTANCE The natural history of MAC-PD is challenging to predict in immunocompetent patients at diagnosis, and the current multidrug chemotherapy options are not strong enough to eliminate mycobacteria from the lungs. Therefore, the diagnosis of MAC-PD does not necessarily lead to the decision to start chemotherapy. We have also observed refractory patients in clinical practice, who were resistant to multiple-drug chemotherapy and showed persistent excretion of MAC bacilli and progressive worsening of chest radiographic findings until death. We have reported that the measurements of anti-MBGL antibody titers helped assess refractory MAC-PD in this study. Furthermore, the predictions of treatment outcome and mortality become more accurate by using the anti-MBGL antibody in addition to clinical poor prognostic characteristics, which were older age, lower body mass index, the positive results of a smear test for acid-fast bacteria (AFB), and presence of cavitary disease.

    DOI: 10.1128/spectrum.00530-22

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  • Monitoring IgG against Mycobacterium tuberculosis proteins in an Asian elephant cured of tuberculosis that developed from long-term latency. 国際誌

    Satoshi Ishikawa, Yuriko Ozeki, Satomi Suga, Yasuhiko Mukai, Haruka Kobayashi, Erina Inouchi, Shaban A Kaboso, Gebremichal Gebretsadik, Desak Nyoman Surya Suameitria Dewi, Akihito Nishiyama, Yoshitaka Tateishi, Hayato Takihara, Shujiro Okuda, Shiomi Yoshida, Naoaki Misawa, Sohkichi Matsumoto

    Scientific reports   12 ( 1 )   4310 - 4310   2022年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Tuberculosis (TB) is fatal in elephants, hence protecting elephants from TB is key not only in the conservation of this endangered animal, but also to prevent TB transmission from elephants to humans. Most human TB cases arise from long-term asymptomatic infections. Significant diagnostic challenges remain in the detection of both infection and disease development from latency in elephants due to their huge bodies. In this study, we assessed cryopreserved sera collected for over 16 years, from the first Japanese treatment case of elephant TB. Semi-quantification of IgG levels to 11 proteins showed high detection levels of 3 proteins, namely ESAT6/CFP10, MPB83 and Ag85B. The level of IgG specific to these 3 antigens was measured longitudinally, revealing high and stable ESAT6/CFP10 IgG levels regardless of onset or treatment. Ag85B-specifc IgG levels were largely responsive to onset or treatment, while those of MPB83 showed intermediate responses. These results suggest that ESAT6/CFP10 is immunodominant in both asymptomatic and symptomatic phases, making it useful in the detection of infection. On the other hand, Ag85B has the potential to be a marker for the prediction of disease onset and in the evaluation of treatment effectiveness in elephants.

    DOI: 10.1038/s41598-022-08228-7

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  • 抗酸菌の新しいタイプのZ、E混成型プレニル基還元酵素の同定と機能解析(Identification and functional analysis of a new type of Z,E-mixed prenyl reductase from mycobacteria)

    阿部 透, 袴田 真理子, 西山 晃史, 立石 善隆, 松本 壮吉, 邊見 久, 上田 大次郎, 佐藤 努

    日本細菌学雑誌   77 ( 1 )   68 - 68   2022年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance. 国際誌

    Aleksandr Ilinov, Akihito Nishiyama, Hiroki Namba, Yukari Fukushima, Hayato Takihara, Chie Nakajima, Anna Savitskaya, Gebremichal Gebretsadik, Mariko Hakamata, Yuriko Ozeki, Yoshitaka Tateishi, Shujiro Okuda, Yasuhiko Suzuki, Yuri S Vinnik, Sohkichi Matsumoto

    Scientific reports   11 ( 1 )   10953 - 10953   2021年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DNA is basically an intracellular molecule that stores genetic information and carries instructions for growth and reproduction in all cellular organisms. However, in some bacteria, DNA has additional roles outside the cells as extracellular DNA (eDNA), which is an essential component of biofilm formation and hence antibiotic tolerance. Mycobacteria include life-threating human pathogens, most of which are slow growers. However, little is known about the nature of pathogenic mycobacteria's eDNA. Here we found that eDNA is present in slow-growing mycobacterial pathogens, such as Mycobacterium tuberculosis, M. intracellulare, and M. avium at exponential growth phase. In contrast, eDNA is little in all tested rapid-growing mycobacteria. The physiological impact of disrupted eDNA on slow-growing mycobacteria include reduced pellicle formation, floating biofilm, and enhanced susceptibility to isoniazid and amikacin. Isolation and sequencing of eDNA revealed that it is identical to the genomic DNA in M. tuberculosis and M. intracellulare. In contrast, accumulation of phage DNA in eDNA of M. avium, suggests that the DNA released differs among mycobacterial species. Our data show important functions of eDNA necessary for biofilm formation and drug tolerance in slow-growing mycobacteria.

    DOI: 10.1038/s41598-021-90156-z

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  • 早期発症者と長期潜伏後発症者より分離した結核菌北京株のゲノム変異解析

    袴田 真理子, 瀧原 速仁, 岩本 朋忠, 田丸 亜貴, 尾関 百合子, 西山 晃史, 立石 善隆, 菊地 利明, 奥田 修二郎, 松本 壮吉

    結核   96 ( 3 )   83 - 86   2021年5月

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    記述言語:日本語   出版者・発行元:(一社)日本結核・非結核性抗酸菌症学会  

    [目的]全ゲノム解析を用いて,早期発症者と長期潜伏後発症者から分離した結核菌北京株のゲノム変異を解析した。[方法]1999年に中学校で発生した結核集団感染の接触者と二次感染者のうち2009年までに結核を発症した患者より分離した結核菌北京株6株と,別の事例で初発時と再発時の患者より分離した結核菌北京株2株の計8株の全ゲノム解析を行った。結核菌の突然変異率は,初発から1年以内に発症した早期発症者と1年以上の潜伏期を経てから発症または再燃した長期潜伏後発症者の2群間で比較した。[結果]結核菌北京株の突然変異率は,Lineage 4に属する結核菌よりも高く,結核菌北京株の高い病原性や薬剤耐性の要因である可能性が示唆された。遺伝子多型解析では,酸化的損傷に起因すると推定されている突然変異が長期潜伏後発症群のほうに多く,潜伏期間中にも薬剤耐性変異が起こる可能性が示唆された。[結論]結核菌北京株の高頻度の薬剤耐性化を防ぐためには,感染した結核菌の系統により治療法を検討する必要性も考えられた。今後,結核菌系統の特性を理解し,治療法を工夫することで結核の薬剤耐性化や重篤化を防ぐ有効な対策の構築につながることが期待される。(著者抄録)

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  • Comparative genomic analysis of Mycobacterium intracellulare: implications for clinical taxonomic classification in pulmonary Mycobacterium avium-intracellulare complex disease. 国際誌

    Yoshitaka Tateishi, Yuriko Ozeki, Akihito Nishiyama, Mari Miki, Ryoji Maekura, Yukari Fukushima, Chie Nakajima, Yasuhiko Suzuki, Sohkichi Matsumoto

    BMC microbiology   21 ( 1 )   103 - 103   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Mycobacterium intracellulare is a representative etiological agent of emerging pulmonary M. avium-intracellulare complex disease in the industrialized countries worldwide. The recent genome sequencing of clinical strains isolated from pulmonary M. avium-intracellulare complex disease has provided insight into the genomic characteristics of pathogenic mycobacteria, especially for M. avium; however, the genomic characteristics of M. intracellulare remain to be elucidated. RESULTS: In this study, we performed comparative genomic analysis of 55 M. intracellulare and related strains such as M. paraintracellulare (MP), M. indicus pranii (MIP) and M. yonogonense. Based on the average nucleotide identity, the clinical M. intracellulare strains were phylogenetically grouped in two clusters: (1) the typical M. intracellulare (TMI) group, including ATCC13950 and virulent M.i.27 and M.i.198 that we previously reported, and (2) the MP-MIP group. The alignment of the genomic regions was mostly preserved between groups. Plasmids were identified between groups and subgroups, including a plasmid common among some strains of the M.i.27 subgroup. Several genomic regions including those encoding factors involved in lipid metabolism (e.g., fadE3, fadE33), transporters (e.g., mce3), and type VII secretion system (genes of ESX-2 system) were shown to be hypermutated in the clinical strains. M. intracellulare was shown to be pan-genomic at the species and subspecies levels. The mce genes were specific to particular subspecies, suggesting that these genes may be helpful in discriminating virulence phenotypes between subspecies. CONCLUSIONS: Our data suggest that genomic diversity among M. intracellulare, M. paraintracellulare, M. indicus pranii and M. yonogonense remains at the subspecies or genovar levels and does not reach the species level. Genetic components such as mce genes revealed by the comparative genomic analysis could be the novel focus for further insight into the mechanism of human pathogenesis for M. intracellulare and related strains.

    DOI: 10.1186/s12866-021-02163-9

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  • 世界結核デーにちなんで、世界の結核・抗酸菌症研究のこれまでと今 結核菌北京株のゲノム解析と組織透明化/3次元イメージング「CUBIC」による抗酸菌感染の生体内モニタリング

    袴田 真理子, 瀧原 速仁, 尾関 百合子, 西山 晃史, 立石 善隆, 大橋 璃子, 奥田 修二郎, 田井中 一貴, 菊地 利明, 松本 壮吉

    日本細菌学雑誌   76 ( 1 )   65 - 65   2021年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • Correction: Significance of a histone-like protein with its native structure for the diagnosis of asymptomatic tuberculosis. 国際誌

    Yukiko Ohara, Yuriko Ozeki, Yoshitaka Tateishi, Tsukasa Mashima, Fumio Arisaka, Yasuo Tsunaka, Yoshie Fujiwara, Akihito Nishiyama, Yutaka Yoshida, Kengo Kitadokoro, Haruka Kobayashi, Yukihiro Kaneko, Ichiro Nakagawa, Ryoji Maekura, Saburo Yamamoto, Masato Katahira, Sohkichi Matsumoto

    PloS one   16 ( 8 )   e0256946   2021年

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    記述言語:英語  

    [This corrects the article DOI: 10.1371/journal.pone.0204160.].

    DOI: 10.1371/journal.pone.0256946

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  • Genome-wide identification of essential genes in Mycobacterium intracellulare by transposon sequencing — Implication for metabolic remodeling

    Yoshitaka Tateishi, Yusuke Minato, Anthony D. Baughn, Hiroaki Ohnishi, Akihito Nishiyama, Yuriko Ozeki, Sohkichi Matsumoto

    Scientific Reports   10 ( 1 )   2020年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    <title>Abstract</title>The global incidence of the human nontuberculous mycobacteria (NTM) disease is rapidly increasing. However, knowledge of gene essentiality under optimal growth conditions and conditions relevant to the natural ecology of NTM, such as hypoxia, is lacking. In this study, we utilized transposon sequencing to comprehensively identify genes essential for growth in <italic>Mycobacterium intracellulare</italic>. Of 5126 genes of <italic>M. intracellulare</italic> ATCC13950, 506 genes were identified as essential genes, of which 280 and 158 genes were shared with essential genes of <italic>M. tuberculosis</italic> and <italic>M. marinum</italic>, respectively. The shared genes included target genes of existing antituberculous drugs including SQ109, which targets the trehalose monomycolate transporter MmpL3. From 175 genes showing decreased fitness as conditionally essential under hypoxia, preferential carbohydrate metabolism including gluconeogenesis, glyoxylate cycle and succinate production was suggested under hypoxia. Virulence-associated genes including proteasome system and mycothiol redox system were also identified as conditionally essential under hypoxia, which was further supported by the higher effective suppression of bacterial growth under hypoxia compared to aerobic conditions in the presence of these inhibitors. This study has comprehensively identified functions essential for growth of <italic>M. intracellulare</italic> under conditions relevant to the host environment. These findings provide critical functional genomic information for drug discovery.

    DOI: 10.1038/s41598-020-62287-2

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    その他リンク: http://www.nature.com/articles/s41598-020-62287-2

  • Higher genome mutation rates of Beijing lineage of Mycobacterium tuberculosis during human infection. 国際誌

    Mariko Hakamata, Hayato Takihara, Tomotada Iwamoto, Aki Tamaru, Atsushi Hashimoto, Takahiro Tanaka, Shaban A Kaboso, Gebremichal Gebretsadik, Aleksandr Ilinov, Akira Yokoyama, Yuriko Ozeki, Akihito Nishiyama, Yoshitaka Tateishi, Hiroshi Moro, Toshiaki Kikuchi, Shujiro Okuda, Sohkichi Matsumoto

    Scientific reports   10 ( 1 )   17997 - 17997   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Mycobacterium tuberculosis (Mtb) strains of Beijing lineage have caused great concern because of their rapid emergence of drug resistance and worldwide spread. DNA mutation rates that reflect evolutional adaptation to host responses and the appearance of drug resistance have not been elucidated in human-infected Beijing strains. We tracked and obtained an original Mtb isolate of Beijing lineage from the 1999 tuberculosis outbreak in Japan, as well as five other isolates that spread in humans, and two isolates from the patient caused recurrence. Three isolates were from patients who developed TB within one year after infection (rapid-progressor, RP), and the other three isolates were from those who developed TB more than one year after infection (slow-progressor, SP). We sequenced genomes of these isolates and analyzed the propensity and rate of genomic mutations. Generation time versus mutation rate curves were significantly higher for RP. The ratio of oxidative versus non-oxidation damages induced mutations was higher in SP than RP, suggesting that persistent Mtb are exposed to oxidative stress in the latent state. Our data thus demonstrates that higher mutation rates of Mtb Beijing strains during human infection is likely to account for the higher adaptability and an emergence ratio of drug resistance.

    DOI: 10.1038/s41598-020-75028-2

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  • 尿プロテオミクスによる肺MAC症のバイオマーカー探索

    横山 晃, 平尾 嘉利, 尾関 百合子, 西山 晃史, 立石 善隆, 桑原 克弘, 菊地 利明, 山本 格, 松本 壮吉

    結核   95 ( 5 )   146 - 146   2020年9月

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    記述言語:日本語   出版者・発行元:(一社)日本結核・非結核性抗酸菌症学会  

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  • Adduct Formation of Delamanid with NAD in Mycobacteria

    Mikayo Hayashi, Akihito Nishiyama, Ryuki Kitamoto, Yoshitaka Tateishi, Mayuko Osada-Oka, Yukiko Nishiuchi, Shaban A. Kaboso, Xiuhao Chen, Mamoru Fujiwara, Yusuke Inoue, Yoshikazu Kawano, Masanori Kawasaki, Tohru Abe, Tsutomu Sato, Kentaro Kaneko, Kimiko Itoh, Sohkichi Matsumoto, Makoto Matsumoto

    Antimicrobial Agents and Chemotherapy   64 ( 5 )   2020年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    Delamanid (DLM), a nitro-dihydroimidazooxazole derivative currently approved for pulmonary multidrug-resistant tuberculosis (TB) therapy, is a prodrug activated by mycobacterial 7,8-didemethyl-8-hydroxy 5-deazaflavin electron transfer coenzyme (F
    <sub>420</sub>
    )-dependent nitroreductase (Ddn). Despite inhibiting the biosynthesis of a subclass of mycolic acids, the active DLM metabolite remained unknown. Comparative liquid chromatography-mass spectrometry (LC-MS) analysis of DLM metabolites revealed covalent binding of reduced DLM with a nicotinamide ring of NAD derivatives (oxidized form) in DLM-treated
    <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
    var.

    DOI: 10.1128/aac.01755-19

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  • Adduct Formation of Delamanid with NAD in Mycobacteria. 査読 国際誌

    Mikayo Hayashi, Akihito Nishiyama, Ryuki Kitamoto, Yoshitaka Tateishi, Mayuko Osada-Oka, Yukiko Nishiuchi, Shaban A Kaboso, Xiuhao Chen, Mamoru Fujiwara, Yusuke Inoue, Yoshikazu Kawano, Masanori Kawasaki, Tohru Abe, Tsutomu Sato, Kentaro Kaneko, Kimiko Itoh, Sohkichi Matsumoto, Makoto Matsumoto

    Antimicrobial agents and chemotherapy   64 ( 5 )   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Delamanid (DLM), a nitro-dihydroimidazooxazole derivative currently approved for pulmonary multidrug-resistant tuberculosis (TB) therapy, is a prodrug activated by mycobacterial 7,8-didemethyl-8-hydroxy 5-deazaflavin electron transfer coenzyme (F420)-dependent nitroreductase (Ddn). Despite inhibiting the biosynthesis of a subclass of mycolic acids, the active DLM metabolite remained unknown. Comparative liquid chromatography-mass spectrometry (LC-MS) analysis of DLM metabolites revealed covalent binding of reduced DLM with a nicotinamide ring of NAD derivatives (oxidized form) in DLM-treated Mycobacterium tuberculosis var. Bacille de Calmette et Guérin. Isoniazid-resistant mutations in the type II NADH dehydrogenase gene (ndh) showed a higher intracellular NADH/NAD ratio and cross-resistance to DLM, which were restored by complementation of the mutants with wild-type ndh Our data demonstrated for the first time the adduct formation of reduced DLM with NAD in mycobacterial cells and its importance in the action of DLM.

    DOI: 10.1128/AAC.01755-19

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  • 抗酸菌症治療薬を目指した標的蛋白質の発現と精製

    大原 由貴子, 小林 悠, 尾関 百合子, 西山 晃史, 立石 善隆, 奥田 修二郎, 神谷 重樹, 北所 健悟, 松本 壮吉

    日本細菌学雑誌   75 ( 1 )   74 - 74   2020年1月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • MDP1はM.tuberculosis var BCG株の生存を確固とするための代謝と複製を調節する(MDP1 regulates metabolism and replication ensuring the survival of M. tuberculosis var BCG)

    シャバン・アミナ, 西山 晃史, 立石 善隆, 山口 雄大, 西内 由紀子, 瀧原 速仁, 奥田 修二郎, 松本 壮吉

    日本細菌学雑誌   75 ( 1 )   88 - 88   2020年1月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 病原性抗酸菌における細胞外DNAの存在と抗酸菌の生理におけるその役割(Existence of extracellular DNA in pathogenic mycobacteria and its role in mycobacterial physiology)

    イリノフ・アレクサンドル, シャバン・アミナ, 袴田 真理子, 西山 晃史, 尾関 百合子, 福島 由華里, 中島 千絵, 立石 善隆, 鈴木 定彦, 松本 壮吉

    日本細菌学雑誌   75 ( 1 )   74 - 74   2020年1月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 早期発症者と長期潜伏後発症者より分離した結核菌北京株のゲノム変異についての解析

    袴田 真理子, 瀧原 速仁, 岩本 朋忠, 田丸 亜貴, 尾関 百合子, 西山 晃史, 立石 善隆, 菊地 利明, 奥田 修二郎, 松本 壮吉

    日本細菌学雑誌   75 ( 1 )   129 - 129   2020年1月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • Characteristic profile of antibody responses to PPD, ESAT-6, and CFP-10 of Mycobacterium tuberculosis in pulmonary tuberculosis suspected cases in Surabaya, Indonesia

    Desak Nyoman Surya Suameitria Dewi, Ni Made Mertaniasih, Soedarsono, Yuriko Ozeki, Wayan Tunas Artama, Fihiruddin, Mamiko Niki, Yoshitaka Tateishi, Manabu Ato, Sohkichi Matsumoto

    The Brazilian Journal of Infectious Diseases   23 ( 4 )   246 - 253   2019年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.bjid.2019.07.001

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  • Metabolic adaptation to glycolysis is a basic defense mechanism of macrophages for Mycobacterium tuberculosis infection. 査読

    Osada-Oka M, Goda N, Saiga H, Yamamoto M, Takeda K, Ozeki Y, Yamaguchi T, Soga T, Tateishi Y, Miura K, Okuzaki D, Kobayashi K, Matsumoto S

    International immunology   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/intimm/dxz048

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  • Serum antibody profiles in individuals with latent Mycobacterium tuberculosis infection. 査読

    Maekura R, Kitada S, Osada-Oka M, Tateishi Y, Ozeki Y, Fujicawa T, Miki M, Jyunnko O, Mori M, Matsumoto S

    Microbiology and immunology   63 ( 3-4 )   130 - 138   2019年3月

  • C-terminal intrinsically disordered region-dependent organization of the mycobacterial genome by a histone-like protein 査読

    Savitskaya Anna, Nishiyama Akihito, Yamaguchi Takehiro, Tateishi Yoshitaka, Ozeki Yuriko, Nameta Masaaki, Kon Tomohiro, Kaboso Shaban A, Ohara Naoya, Peryanova Olga V, Matsumoto Sohkichi

    SCIENTIFIC REPORTS   8 ( 1 )   8197   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-018-26463-9

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  • Significance of a histone-like protein with its native structure for the diagnosis of asymptomatic tuberculosis. 査読

    Ohara Y, Ozeki Y, Tateishi Y, Mashima T, Arisaka F, Tsunaka Y, Fujiwara Y, Nishiyama A, Yoshida Y, Kitadokoro K, Kobayashi H, Kaneko Y, Nakagawa I, Maekura R, Yamamoto S, Katahira M, Matsumoto S

    PloS ONE   13 ( 10 )   e0204160   2018年

  • Mycobacterial DNA-binding protein 1 is critical for long term survival of Mycobacterium smegmatis and simultaneously coordinates cellular functions 査読

    Shymaa Enany, Yutaka Yoshida, Yoshitaka Tateishi, Yuriko Ozeki, Akihito Nishiyama, Anna Savitskaya, Takehiro Yamaguchi, Yukiko Ohara, Tadashi Yamamoto, Manabu Ato, Sohkichi Matsumoto

    SCIENTIFIC REPORTS   7   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Bacteria can proliferate perpetually without ageing, but they also face conditions where they must persist. Mycobacteria can survive for a long period. This state appears during mycobacterial diseases such as tuberculosis and leprosy, which are chronic and develop after long-term persistent infections. However, the fundamental mechanisms of the long-term living of mycobacteria are unknown. Every Mycobacterium species expresses Mycobacterial DNA-binding protein 1 (MDP1), a histone-like nucleoid associated protein. Mycobacterium smegmatis is a saprophytic fast grower and used as a model of mycobacterial persistence, since it shares the characteristics of the long-term survival observed in pathogenic mycobacteria. Here we show that MDP1-deficient M. smegmatis dies more rapidly than the parental strain after entering stationary phase. Proteomic analyses revealed 21 upregulated proteins with more than 3-fold in MDP1-deficient strain, including DnaA, a replication initiator, NDH, a NADH dehydrogenase that catalyzes downhill electron transfer, Fas1, a critical fatty acid synthase, and antioxidants such as AhpC and KatG. Biochemical analyses showed elevated levels of DNA and ATP syntheses, a decreased NADH/NAD(+) ratio, and a loss of resistance to oxidative stress in the MDP1-knockout strain. This study suggests the importance of MDP1-dependent simultaneous control of the cellular functions in the long-term survival of mycobacteria.

    DOI: 10.1038/s41598-017-06480-w

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  • Effects of nutritional and ambient oxygen condition on biofilm formation in Mycobacterium avium subsp hominissuis via altered glycolipid expression 査読

    Takahiro Totani, Yukiko Nishiuchi, Yoshitaka Tateishi, Yutaka Yoshida, Hiromi Kitanaka, Mamiko Niki, Yukihiro Kaneko, Sohkichi Matsumoto

    SCIENTIFIC REPORTS   7   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Mycobacterium avium subsp. hominissuis (MAH) is the major causative agent of nontuberculous mycobacteriosis, the representative case of environment-related infectious diseases the incidence of which is increasing in industrialized countries. MAH is found in biofilm in drinking water distribution system and residential environments. We investigated the effect of gaseous and nutritional conditions, and the role of glycopeptidolipids (GPLs) on biofilm-like pellicle formation in MAH. Pellicle formation was observed under 5% oxygen in Middlebrook 7H9 broth containing 0.2% glycerol and 10% albumindextrose- catalase enrichment but not under normoxia or in nutrient- poor media. An analysis of 17 environmental isolates revealed that hypoxia (5% oxygen) preferentially enhanced pellicle formation both in plastic plates and in glass tubes, compared with hypercapnia (5% carbon dioxide). Wild-type strains (WT) developed much thicker pellicles than GPL-deficient rough mutants (RM). WT bacterial cells distributed randomly and individually in contrast to that RM cells positioned linearly in a definite order. Exogenous supplementation of GPLs thickened the pellicles of RM, resulting in a similar morphological pattern to WT. These data suggest a significant implication of eutrophication and hypoxia in biofilm-like pellicle formation, and a functional role of GPLs on development of pellicles in MAH.

    DOI: 10.1038/srep41775

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  • A new screen for tuberculosis drug candidates utilizing a luciferase-expressing recombinant Mycobacterium bovis Bacillus Calmette-Guéren 査読

    Ozeki Y, Igarashi M, Doe M, Tamaru A, Kinoshita N, Ogura Y, Iwamoto T, Sawa R, Umekita M, Enany S, Nishiuchi Y, Osada-Oka M, Hayashi T, Niki M, Tateishi Y, Hatano M, Matsumoto S

    PLoS One   10 ( 11 )   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1371/journal.pone.0141658

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  • Identification of Three Exercise-induced Mortality Risk Factors in Patients with COPD 査読

    Kenji Yoshimura, Ryoji Maekura, Toru Hiraga, Keisuke Miki, Seigo Kitada, Mari Miki, Yoshitaka Tateishi, Masahide Mori

    COPD-JOURNAL OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE   11 ( 6 )   615 - 626   2014年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INFORMA HEALTHCARE  

    The survival rate of chronic obstructive pulmonary disease (COPD) patients with severely reduced exercise capacity is extremely low. We recently identified three life-threatening pathophysiological conditions during cardiopulmonary exercise testing (CPET): (1) exercise-induced hypoxemia, (2) sympathetic overactivity, and (3) progressive respiratory acidosis at low-intensity exercise. The present prospective observation study aimed to determine whether these parameters constitute risk factors of mortality in moderate-to-very severe COPD. Ninety-six COPD patients were followed-up, monthly, for &gt; 3 years. Subsequently, spirometry and CPET were performed to examine parameters of exercise-induced hypoxemia ([PaO2 slope, mmHg/L.min(-1)] = Decrease in PaO2/Delta VO2 (Difference in Delta VO2 between at rest and at peak exercise)), progression of acidosis ([Delta pH/Delta VO2/L.min(-1)] = Decrease in pH/Delta VO2), and sympathetic overactivity ([Delta norepinephrine(NE)/Delta VO2, ng/mL/L.min(-1)] = increase in NE/Delta VO2). Univariate analysis revealed a significant association between the three conditions with increased mortality. Kaplan-Meier analysis showed that the quartile combining the steepest PaO2 slope (&lt;=-55 mmHg/Delta VO2 [L/min]), steepest decrease in arterial blood pH (&lt;= -1.72/Delta VO2 [L/min]), and most rapid increase in plasma NE level (&gt;= 5.2 ng/Delta VO2 [L/min]) during incremental exercise was associated with higher all-cause mortality. These conditions showed cumulative effects on COPD patients' survival. Multivariate analyses revealed that these three life-threatening factors are also independent predictors of mortality based on age, heart rate and PaO2 at rest, body mass index, and forced expiratory volume in 1 s. Thus, these new exercise-induced mortality risk factors may lead to more efficient pulmonary rehabilitation programs for COPD patients based on patient-specific exercise-induced pathophysiological profiles.

    DOI: 10.3109/15412555.2014.898038

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  • Whole-Genome Sequence of Mycobacterium kyorinense. 査読

    Ohtsuka K, Ohnishi H, Nozaki E, Pais Ramos J, Tortoli E, Yonetani S, Matsushima S, Tateishi Y, Matsumoto S, Watanabe T

    Genome announcements   2 ( 5 )   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1128/genomeA.01062-14

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  • Differences in Physiological Response to Exercise in Patients With Different COPD Severity 査読

    Ryoji Maekura, Toru Hiraga, Keisuke Miki, Seigo Kitada, Kenji Yoshimura, Mari Miki, Yoshitaka Tateishi

    RESPIRATORY CARE   59 ( 2 )   252 - 262   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:DAEDALUS ENTERPRISES INC  

    BACKGROUND: Patients with COPD have reduced exercise tolerance associated with dyspnea. This exercise intolerance is primarily due to impaired ventilatory mechanics, but it is also associated with a combination of factors, including inefficient gas exchange, lactic acidosis at a low work rate, and exercise-induced hypoxemia. The survival prognosis of COPD patients with severely reduced exercise capacity is extremely poor, but the pathophysiology of these patients during exercise remains to be accurately established. The present study aimed to characterize life-threatening factors such as hypoxemia, acidosis, and sympathetic activation during exercise in these patients. METHODS: We monitored changes in life-threatening factors and compared these factors among quartile groups, defined according to their peak oxygen uptake status. Ninety-one COPD subjects (82 males, 9 females, average age 69.7 +/- 6.8 y) consecutively underwent incremental cardiopulmonary exercise testing using a cycle ergometer. Arterial blood gases, lactate, and catecholamines were measured during cardiopulmonary exercise testing. RESULTS: The pathophysiology of the COPD differed among the 4 subject groups. Subjects with the most severely reduced exercise capacity (peak oxygen uptake &lt;= 623 mL/min) were characterized by exercise-induced steep decrease in P-aO2 slope (-78 +/- 70 mm Hg/L/min), rapid progression of respiratory acidosis, little change in lactic acidosis, and sympathetic activation at low-intensity work load (plasma norepinephrine 1.41 +/- 0.94 ng/mL at 20 watts work load), in addition to the limitation of increase in ventilation and impaired gas exchange. CONCLUSIONS: The mechanisms of exercise intolerance in COPD patients significantly differed among subjects with different exercise capacities. Subjects with the most severely reduced exercise capacity had the characteristics of exercise-induced hypoxemia, sympathetic overactivity, and progressive respiratory acidosis at low-intensity exercise. These life-threatening pathophysiological conditions could be improved by medication and/or pulmonary rehabilitation.

    DOI: 10.4187/respcare.02201

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  • Differences in Physiological Response to Exercise in Patients With Different COPD Severity 査読

    Ryoji Maekura, Toru Hiraga, Keisuke Miki, Seigo Kitada, Kenji Yoshimura, Mari Miki, Yoshitaka Tateishi

    RESPIRATORY CARE   59 ( 2 )   252 - 262   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:DAEDALUS ENTERPRISES INC  

    BACKGROUND: Patients with COPD have reduced exercise tolerance associated with dyspnea. This exercise intolerance is primarily due to impaired ventilatory mechanics, but it is also associated with a combination of factors, including inefficient gas exchange, lactic acidosis at a low work rate, and exercise-induced hypoxemia. The survival prognosis of COPD patients with severely reduced exercise capacity is extremely poor, but the pathophysiology of these patients during exercise remains to be accurately established. The present study aimed to characterize life-threatening factors such as hypoxemia, acidosis, and sympathetic activation during exercise in these patients. METHODS: We monitored changes in life-threatening factors and compared these factors among quartile groups, defined according to their peak oxygen uptake status. Ninety-one COPD subjects (82 males, 9 females, average age 69.7 +/- 6.8 y) consecutively underwent incremental cardiopulmonary exercise testing using a cycle ergometer. Arterial blood gases, lactate, and catecholamines were measured during cardiopulmonary exercise testing. RESULTS: The pathophysiology of the COPD differed among the 4 subject groups. Subjects with the most severely reduced exercise capacity (peak oxygen uptake &lt;= 623 mL/min) were characterized by exercise-induced steep decrease in P-aO2 slope (-78 +/- 70 mm Hg/L/min), rapid progression of respiratory acidosis, little change in lactic acidosis, and sympathetic activation at low-intensity work load (plasma norepinephrine 1.41 +/- 0.94 ng/mL at 20 watts work load), in addition to the limitation of increase in ventilation and impaired gas exchange. CONCLUSIONS: The mechanisms of exercise intolerance in COPD patients significantly differed among subjects with different exercise capacities. Subjects with the most severely reduced exercise capacity had the characteristics of exercise-induced hypoxemia, sympathetic overactivity, and progressive respiratory acidosis at low-intensity exercise. These life-threatening pathophysiological conditions could be improved by medication and/or pulmonary rehabilitation.

    DOI: 10.4187/respcare.02201

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  • Direct detection of Mycobacterium avium in environmental water and scale samples by loop-mediated isothermal amplification 査読

    Yukiko Nishiuchi, Aki Tamaru, Yasuhiko Suzuki, Seigo Kitada, Ryoji Maekura, Yoshitaka Tateishi, Mamiko Niki, Hisashi Ogura, Sohkichi Matsumoto

    JOURNAL OF WATER AND HEALTH   12 ( 2 )   211 - 219   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IWA PUBLISHING  

    We previously demonstrated the colonization of Mycobacterium avium complex in bathrooms by the conventional culture method. In the present study, we aimed to directly detect M. avium organisms in the environment using loop-mediated isothermal amplification (LAMP), and to demonstrate the efficacy of LAMP by comparing the results with those obtained by culture. Our data showed that LAMP analysis has detection limits of 100 fg DNA/reaction for M. avium. Using an FTA (R) elute card,DNA templates were extracted from environmental samples from bathrooms in the residences of 29 patients with pulmonary M. avium disease. Of the 162 environmental samples examined, 143 (88%) showed identical results by both methods; 20 (12%) and 123 (76%) samples were positive and negative, respectively, for M. avium. Of the remaining 19 samples (12%), seven (5%) and 12 (7%) samples were positive by the LAMP and culture methods, respectively. All samples that contained over 20 colony forming units/primary isolation plate, as measured by the culture method, were also positive by the LAMP method. Our data demonstrate that the combination of the FTA elute card and LAMP can facilitate prompt detection of M. avium in the environment.

    DOI: 10.2166/wh.2013.007

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  • Effects of Ghrelin Treatment on Exercise Capacity in Underweight COPD Patients: a substudy of a multicenter, randomized, double-blind, placebo-controlled trial of ghrelin treatment 査読

    Keisuke Miki, Ryoji Maekura, Noritoshi Nagaya, Seigo Kitada, Mari Miki, Kenji Yoshimura, Yoshitaka Tateishi, Masaharu Motone, Toru Hiraga, Masahide Mori, Kenji Kangawa

    BMC PULMONARY MEDICINE   13   37 - 46   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: The aim of this substudy of the ghrelin treatment, multicenter, randomized, double-blind, placebo-controlled trial was to investigate the effects of ghrelin administration on exercise capacity and the underlying mechanisms in underweight patients with chronic obstructive pulmonary disease (COPD) using cardiopulmonary exercise testing.
    Methods: Twenty underweight COPD patients were randomized to pulmonary rehabilitation with intravenous ghrelin (2 mu g/kg, n = 10) or placebo (n = 10) twice daily for 3 weeks in a double-blind fashion. The primary outcome was changes in peak oxygen uptake ((V) over dot O-2). Secondary outcomes included changes in exertional cardio-respiratory functions: O-2-pulse, physiologic dead space/tidal volume-ratio (V-D/V-T), ventilatory equivalent for oxygen ((V) over dot(E)/(V) over dotO(2)), and ventilatory equivalent for carbon dioxide ((V) over dot(E)//(V) over dotCO(2)).
    Results: With incremental exercise, at peak exercise, there was a significant difference in the mean difference (ghrelin minus placebo), i.e., treatment effect in: i) peak (V) over dotO(2) (1.2 mL/kg/min, 95% CI: 0.2-2.3 mL/kg/min, between-group p = 0.025); ii) (V) over dot(E)/(V) over dotO(2) (-4.2, 95% CI: -7.9 to -0.5, between-group p = 0.030); iii) (V) over dot(E)/(V) over dotCO(2) (-4.1, 95% CI: -8.2 to -0.1, between-group p = 0.045); iv) V-D/V-T (-0.04, 95% CI: -0.08 to -0.00, between-group p = 0.041); and v) O-2-pulse (0.7 mL/beat, 95% CI: 0.3 to 1.2 mL/beat, between-group p = 0.003). Additionally, repeated-measures analysis of variance (ANOVA) indicated a significant time-course effect of ghrelin versus placebo in the peak (V) over dotO(2) (p = 0.025).
    Conclusion: Ghrelin administration was associated with improved exertional capacity and improvements in ventilatory-cardiac parameters.

    DOI: 10.1186/1471-2466-13-37

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  • Exertional acidotic responses in idiopathic pulmonary fibrosis: the mechanisms of exertional dyspnea. 査読 国際誌

    Keisuke Miki, Ryoji Maekura, Mari Miki, Seigo Kitada, Kenji Yoshimura, Yoshitaka Tateishi, Masahide Mori

    Respiratory physiology & neurobiology   185 ( 3 )   653 - 8   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To understand the mechanism of exertional dyspnea, we postulated that, despite hyperoxia during exercise, patients with idiopathic pulmonary fibrosis (IPF) might not regulate exertional acidosis by ventilatory compensation to stop exercise. The exercise responses during 30% O(2) or compressed air (CA) were examined in 13 patients with IPF. The PaO(2), PaCO(2), and HCO(3)(-) levels were higher during exercise with hyperoxia than with CA. At peak exercise, hyperoxia reduced the plasma lactate level. The dyspnea-ratio (%) of the ΔV(O(2)) (peak minus resting oxygen uptake) curve reached a break point that occurred at a similar exercise point with hyperoxia and CA, preceded by a break point in the breathing frequency-ratio of the ΔV(O(2)). Accordingly, the dyspnea score and pH each reached similar levels with hyperoxia and CA to stop exercise. Regardless of breathing CA or 30% O(2), IPF patients did not regulate exertional acidosis by ventilatory compensation to stop exercise, resulting in reaching a specific pH.

    DOI: 10.1016/j.resp.2012.11.008

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  • Antigen 85A and mycobacterial DNA-binding protein 1 are targets of immunoglobulin G in individuals with past tuberculosis 査読

    Mayuko Osada-Oka, Yoshitaka Tateishi, Yukio Hirayama, Yuriko Ozeki, Mamiko Niki, Seigo Kitada, Ryoji Maekura, Kunio Tsujimura, Yukio Koide, Naoya Ohara, Taro Yamamoto, Kazuo Kobayashi, Sohkichi Matsumoto

    MICROBIOLOGY AND IMMUNOLOGY   57 ( 1 )   30 - 37   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Development of accurate methods for predicting progression of tuberculosis (TB) from the latent state is recognized as vitally important in controlling TB, because a majority of cases develop from latent infections. Past TB that has never been treated has a higher risk of progressing than does latent Mycobacterium tuberculosis infection in patients who have previously received treatment. Antibody responses against 23 kinds of M. tuberculosis proteins in individuals with past TB who had not been medicated were evaluated. These individuals had significantly higher concentrations of antibodies against Antigen 85A and mycobacterial DNA-binding protein 1 (MDP1) than did those with active TB and uninfected controls. In addition, immunohistochemistry revealed colocalization of tubercle bacilli, antigen 85 and MDP1 inside tuberculous granuloma lesions in an asymptomatic subject, showing that M. tuberculosis in lesions expresses both antigen 85 and MDP1. Our study suggests the potential usefulness of measuring antibody responses to antigen 85A and MDP1 for assessing the risk of TB progression.

    DOI: 10.1111/j.1348-0421.2012.12005.x

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  • Antigen 85A and mycobacterial DNA-binding protein 1 are targets of immunoglobulin G in individuals with past tuberculosis.

    Osada-Oka Mayuko, Tateishi Yoshitaka, Hirayama Yukio, Ozeki Yuriko, Niki Mamiko, Kitada Seigo, Maekura Ryoji, Tsujimura Kunio, Koide Yukio, Ohara Naoya, Yamamoto Taro, Kobayashi Kazuo, Matsumoto Sohkichi

    Microbiol Immunol   57 ( 1 )   30 - 37   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Development of accurate methods for predicting progression of tuberculosis (TB) from the latent state is recognized as vitally important in controlling TB, because a majority of cases develop from latent infections. Past TB that has never been treated has a higher risk of progressing than does latent Mycobacterium tuberculosis infection in patients who have previously received treatment. Antibody responses against 23 kinds of M. tuberculosis proteins in individuals with past TB who had not been medicated were evaluated. These individuals had significantly higher concentrations of antibodies against Antigen 85A and mycobacterial DNA-binding protein 1 (MDP1) than did those with active TB and uninfected controls. In addition, immunohistochemistry revealed colocalization of tubercle bacilli, antigen 85 and MDP1 inside tuberculous granuloma lesions in an asymptomatic subject, showing that M. tuberculosis in lesions expresses both antigen 85 and MDP1. Our study suggests the potential usefulness of measuring antibody responses to antigen 85A and MDP1 for assessing the risk of TB progression.

    DOI: 10.1111/j.1348-0421.2012.12005.x

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  • Whole-genome sequence of the potentially hypertransmissible multidrug-resistant Mycobacterium tuberculosis Beijing strain OM-V02_005 査読

    Yoshitaka Tateishi, Aki Tamaru, Yoshitoshi Ogura, Mamiko Niki, Takayuki Wada, Taro Yamamoto, Kazuto Hirata, Tetsuya Hayashi, Sohkichi Matsumoto

    Genome Announcements   1 ( 4 )   00608 - 00613   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    We report the draft genome sequence of Mycobacterium tuberculosis Beijing strain OM-V02_005, which exhibits possible hypertransmissible characteristics among the population of patients with multidrug-resistant tuberculosis in Osaka Prefecture, the largest urban area in western Japan.

    DOI: 10.1128/genomeA.00608-13

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  • Whole-genome sequence of the potentially hypertransmissible multidrug-resistant Mycobacterium tuberculosis Beijing strain OM-V02_005 査読

    Yoshitaka Tateishi, Aki Tamaru, Yoshitoshi Ogura, Mamiko Niki, Takayuki Wada, Taro Yamamoto, Kazuto Hirata, Tetsuya Hayashi, Sohkichi Matsumoto

    Genome Announcements   1 ( 4 )   e00608 - e00613   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    We report the draft genome sequence of Mycobacterium tuberculosis Beijing strain OM-V02_005, which exhibits possible hypertransmissible characteristics among the population of patients with multidrug-resistant tuberculosis in Osaka Prefecture, the largest urban area in western Japan.

    DOI: 10.1128/genomeA.00608-13

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  • Whole-Genome Sequence of the Hypervirulent Clinical Strain Mycobacterium intracellulare M.i.198 査読

    Yoshitaka Tateishi, Seigo Kitada, Keisuke Miki, Ryoji Maekura, Yoshitoshi Ogura, Yuriko Ozeki, Yukiko Nishiuchi, Mamiko Niki, Tetsuya Hayashi, Kazuto Hirata, Kazuo Kobayashi, Sohkichi Matsumoto

    JOURNAL OF BACTERIOLOGY   194 ( 22 )   6336 - 6336   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    We report herein the draft genome sequence of Mycobacterium intracellulare clinical strain M.i.198, which consistently exhibits hypervirulence in human patients, human macrophages in vitro, and immunocompetent mice.

    DOI: 10.1128/JB.01439-12

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  • Whole-genome sequence of the hypervirulent clinical strain Mycobacterium intracellulare M.i.198.

    Tateishi Yoshitaka, Kitada Seigo, Miki Keisuke, Maekura Ryoji, Ogura Yoshitoshi, Ozeki Yuriko, Nishiuchi Yukiko, Niki Mamiko, Hayashi Tetsuya, Hirata Kazuto, Kobayashi Kazuo, Matsumoto Sohkichi

    J Bacteriol   194 ( 22 )   6336 - 6336   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We report herein the draft genome sequence of Mycobacterium intracellulare clinical strain M.i.198, which consistently exhibits hypervirulence in human patients, human macrophages in vitro, and immunocompetent mice.

    DOI: 10.1128/JB.01439-12

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  • A Novel Mechanism of Growth Phase-dependent Tolerance to Isoniazid in Mycobacteria 査読

    Makoto Niki, Mamiko Niki, Yoshitaka Tateishi, Yuriko Ozeki, Teruo Kirikae, Astrid Lewin, Yusuke Inoue, Makoto Matsumoto, John L. Dahl, Hisashi Ogura, Kazuo Kobayashi, Sohkichi Matsumoto

    JOURNAL OF BIOLOGICAL CHEMISTRY   287 ( 33 )   27743 - 27752   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Tuberculosis remains one of the most deadly infectious diseases worldwide and is a leading public health problem. Although isoniazid (INH) is a key drug for the treatment of tuberculosis, tolerance to INH necessitates prolonged treatment, which is a concern for effective tuberculosis chemotherapy. INH is a prodrug that is activated by the mycobacterial enzyme, KatG. Here, we show that mycobacterial DNA-binding protein 1 (MDP1), which is a histone-like protein conserved in mycobacteria, negatively regulates katG transcription and leads to phenotypic tolerance to INH in mycobacteria. Mycobacterium smegmatis deficient for MDP1 exhibited increased expression of KatG and showed enhanced INH activation compared with the wild-type strain. Expression of MDP1 was increased in the stationary phase and conferred growth phase-dependent tolerance to INH in M. smegmatis. Regulation of KatG expression is conserved between M. smegmatis and Mycobacterium tuberculosis complex. Artificial reduction of MDP1 in Mycobacterium bovis BCG was shown to lead to increased KatG expression and susceptibility to INH. These data suggest a mechanism by which phenotypic tolerance to INH is acquired in mycobacteria.

    DOI: 10.1074/jbc.M111.333385

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  • Long-term radiographic outcome of nodular bronchiectatic Mycobacterium avium complex pulmonary disease 査読

    Seigo Kitada, T. Uenami, K. Yoshimura, Y. Tateishi, K. Miki, M. Miki, H. Hashimoto, T. Fujikawa, M. Mori, K. Matsuura, M. Kuroyama, R. Maekura

    International Journal of Tuberculosis and Lung Disease   16 ( 5 )   660 - 664   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Although Mycobacterium avium complex pulmonary disease (MAC-PD) is a growing health problem, little is known about long-term radiographic outcome and factors for deterioration in patients with MAC-PD. METHODS: Data on patients with nodular bronchiectatic (NBE) MAC-PD who underwent regular follow-up for &gt
    5 years were retrospectively reviewed. Changes in plain chest radiograph (CXR) and baseline characteristics were compared between the stable and deteriorated groups. RESULTS: Seventy-two patients were investigated, including 30 patients who were examined 10 years after the initial visit. One patient (1.4%) showed progressive or remarkably progressive disease on CXR at 1 year
    this rate increased to 22.2% at 5 years and to 53.3% at 10 years. Body mass index (BMI) at the initial visit was lower in the deteriorated group than in the stable group. Cavitary disease and resistance to a macrolide were seen more frequently at the initial visit in the deteriorated group than in the stable group. CONCLUSIONS: NBE MAC-PD is a slowly but substantially progressive long-term infection (5-10 years). Our data suggest that patients with lower BMI, cavitary disease and resistance to a macrolide at initial visit are more likely to progress to deteriorating disease. © 2012 The Union.

    DOI: 10.5588/ijtld.11.0534

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  • Ghrelin Treatment of Cachectic Patients with Chronic Obstructive Pulmonary Disease: A Multicenter, Randomized, Double-Blind, Placebo-Controlled Trial 査読

    Keisuke Miki, Ryoji Maekura, Noritoshi Nagaya, Masamitsu Nakazato, Hiroshi Kimura, Shinsuke Murakami, Shunsuke Ohnishi, Toru Hiraga, Mari Miki, Seigo Kitada, Kenji Yoshimura, Yoshitaka Tateishi, Yasuji Arimura, Nobuhiro Matsumoto, Masanori Yoshikawa, Kenichi Yamahara, Kenji Kangawa

    PLOS ONE   7 ( 5 )   e35708   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Background: Pulmonary cachexia is common in advanced chronic obstructive pulmonary disease (COPD), culminating in exercise intolerance and a poor prognosis. Ghrelin is a novel growth hormone (GH)-releasing peptide with GH-independent effects. The efficacy and safety of adding ghrelin to pulmonary rehabilitation (PR) in cachectic COPD patients were investigated.
    Methodology/Principal Findings: In a multicenter, randomized, double-blind, placebo-controlled trial, 33 cachectic COPD patients were randomly assigned PR with intravenous ghrelin (2 mg/kg) or placebo twice daily for 3 weeks in hospital. The primary outcomes were changes in 6-min walk distance (6-MWD) and the St. George Respiratory Questionnaire (SGRQ) score. Secondary outcomes included changes in the Medical Research Council (MRC) scale, and respiratory muscle strength. At pre-treatment, serum GH levels were increased from baseline levels by a single dose of ghrelin (mean change, +46.5 ng/ml; between-group p&lt;0.0001), the effect of which continued during the 3-week treatment. In the ghrelin group, the mean change from pre-treatment in 6-MWD was improved at Week 3 (+40 m, within-group p = 0.033) and was maintained at Week 7 (+47 m, within-group p = 0.017), although the difference between ghrelin and placebo was not significant. At Week 7, the mean changes in SGRQ symptoms (between-group p = 0.026), in MRC (between-group p = 0.030), and in maximal expiratory pressure (MEP; between-group p = 0.015) were better in the ghrelin group than in the placebo group. Additionally, repeated-measures analysis of variance (ANOVA) indicated significant time course effects of ghrelin versus placebo in SGRQ symptoms (p = 0.049) and MEP (p = 0.021). Ghrelin treatment was well tolerated.
    Conclusions/Significance: In cachectic COPD patients, with the safety profile, ghrelin administration provided improvements in symptoms and respiratory strength, despite the lack of a significant between-group difference in 6-MWD.

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  • Effects of oxygen on exertional dyspnoea and exercise performance in patients with chronic obstructive pulmonary disease 査読

    Keisuke Miki, Ryoji Maekura, Toru Hiraga, Seigo Kitada, Mari Miki, Kenji Yoshimura, Yoshitaka Tateishi

    RESPIROLOGY   17 ( 1 )   149 - 154   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Background and objective: The results of studies on the oxygen response in patients with COPD should provide important clues to the pathophysiology of exertional dyspnoea. We investigated the exercise responses to hyperoxia in relation to dyspnoea profile, as well as cardiopulmonary, acidotic and sympathetic parameters in 35 patients with stable COPD (mean FEV1 46% predicted).
    Methods: This was a single-blind trial, in which patients breathed 24% O-2 or compressed air (CA) in random order during two incremental cycle exercise tests.
    Results: PaO2 and PaCO2 were higher (P &lt; 0.0001 and P &lt; 0.05, respectively) at each exercise point while patients were breathing 24% O-2 compared with CA. At a standardized time point near peak exercise, use of O-2 resulted in reduced plasma lactate and plasma noradrenaline concentrations (P &lt; 0.01). Peak minute ventilation/indirect maximum voluntary ventilation was similar while breathing 24% O-2 and CA. At peak exercise, the dyspnoea score, pH and plasma noradrenaline concentrations were similar while breathing 24% O-2 and CA. The dyspnoea-ratio (%) of Delta oxygen uptake (peak minus resting oxygen uptake) curve reached a break point that occurred at a similar exercise point while breathing 24% O-2 or CA.
    Conclusions: Regardless of whether they breathed CA or 24% O-2, patients with COPD did not develop ventilatory compensation for exertional acidosis, and the pH values measured were similar. Hyperoxia during a standardized exercise protocol did not alter the pattern of exertional dyspnoea in these patients, compared with breathing CA, although hyperoxia resulted in miscellaneous effects.

    DOI: 10.1111/j.1440-1843.2011.02086.x

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  • Effects of tiotropium on sympathetic activation during exercise in stable chronic obstructive pulmonary disease patients 査読

    Kenji Yoshimura, Ryoji Maekura, Toru Hiraga, Seigo Kitada, Keisuke Miki, Mari Miki, Yoshitaka Tateishi

    INTERNATIONAL JOURNAL OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE   7   109 - 118   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:DOVE MEDICAL PRESS LTD  

    Background: Tiotropium partially relieves exertional dyspnea and reduces the risk of congestive heart failure in chronic obstructive pulmonary disease (COPD) patients. However, its effect on the sympathetic activation response to exercise is unknown.
    Aims: This study aimed to determine whether tiotropium use results in a sustained reduction in sympathetic activation during exercise.
    Methods: We conducted a 12-week, open-label (treatments: tiotropium 18 mu g or oxitropium 0.2 mg x 3 mg), crossover study in 17 COPD patients. Treatment order was randomized across subjects. The subjects underwent a pulmonary function test and two modes of cardiopulmonary exercise (constant work rate and incremental exercise) testing using a cycle ergometer, with measurement of arterial catecholamines after each treatment period.
    Results: Forced expiratory volume in 1 second and forced vital capacity were significantly larger in the tiotropium treatment group. In constant exercise testing, exercise endurance time was longer, with improvement in dyspnea during exercise and reduction in dynamic hyperinflation in the tiotropium treatment group. Similarly, in incremental exercise testing, exercise time, carbon dioxide production, and minute ventilation at peak exercise were significantly higher in the tiotropium treatment group. Plasma norepinephrine concentrations and dyspnea intensity were also lower during submaximal isotime exercise and throughout the incremental workload exercise in the tiotropium treatment group.
    Conclusion: Tiotropium suppressed the increase of sympathetic activation during exercise at the end of the 6-week treatment, as compared with the effect of oxipropium. This effect might be attributed to improvement in lung function and exercise capacity and reduction in exertional dyspnea, which were associated with decreases in respiratory frequency and heart rate and reduced progression of arterial acidosis.

    DOI: 10.2147/COPD.S28677

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  • Impaired muscle oxygenation during incremental cycle exercise in COPD patients compared with age-matched healthy subjects. 査読

    Tateishi Y, Eguchi Y, Tohyama Y, Hirata K, Fujimoto S

    Open Rehabil J.   4   32 - 41   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • [Susceptibility to rifabutin and novel fluoroquinolones in Mycobacterium avium complex isolates from patients with sputum culture-positive pulmonary disease who are undergoing standard chemotherapy].

    Yoshitaka Tateishi, Masaharu Motone, Kenji Yoshimura, Mari Miki, Keisuke Miki, Seigo Kitada, Hisako Hashimoto, Toru Hiraga, Ryoji Maekura

    Nihon Kokyuki Gakkai zasshi = the journal of the Japanese Respiratory Society   48 ( 11 )   797 - 802   2010年11月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    We investigated the susceptibility to conventional and newer antimycobacterial agents including rifabutin (RBT) and novel fluoroquinolones (NFQs) among 48 clinical Mycobacterium avium complex (MAC) isolates from patients with sputum culture-positive MAC disease who were undergoing standard chemotherapy. RBT and NFQs were superior to conventional agents because of higher rates of susceptibility and lower minimum inhibitory concentration. NFQs showed cross-resistance among quinolones. In contrast, RBT did not show cross-resistance to RFP. Most clarithromycin-resistant or rifampicin-resistant cases were susceptible to RBT and NFQs. In conclusion, RBT and NFQs possess good in vitro antimicrobial activity among clinical isolates of culture-positive pulmonary MAC disease, which suggests that a combination of such microbiologically active agents may improve clinical effectiveness more than standard chemotherapy regimens.

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  • Serodiagnosis of Pulmonary Disease Due to Mycobacterium avium Complex Proven by Bronchial Wash Culture 査読

    Seigo Kitada, Kazuo Kobayashi, Yukiko Nishiuchi, Kenji Fushitani, Kenji Yoshimura, Yoshitaka Tateishi, Keisuke Miki, Mari Miki, Hisako Hashimoto, Masaharu Motone, Takeya Fujikawa, Tarn Hiraga, Ryoji Maekura

    CHEST   138 ( 1 )   236 - 237   2010年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER COLL CHEST PHYSICIANS  

    DOI: 10.1378/chest.10-0248

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  • Exertional dyspnea-related acidotic and sympathetic responses in patients with sequelae of pulmonary tuberculosis 査読

    Keisuke Miki, Ryoji Maekura, Toru Hiraga, Hisako Hashimoto, Seigo Kitada, Mari Miki, Kenji Yoshimura, Yoshitaka Tateishi, Teppei Sugano, Masaharu Motone

    JOURNAL OF PHYSIOLOGICAL SCIENCES   60 ( 3 )   187 - 193   2010年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER JAPAN KK  

    The objective of this study was to investigate whether exertional dyspnea correlates with exercise responses, especially arterial blood pH and plasma norepinephrine (NE) changes, in patients with sequelae of tuberculosis (TBsq). Cardiopulmonary exercise testings were performed in 49 TBsq patients and 9 controls. Each group had a break point in the dyspnea, plasma lactate, and plasma NE changes during exercise, all of which occurred at a similar exercise point. In TBsq patients in both exercise phases before and after the dyspnea break point, the dyspnea-slope (a dagger Borg scale/a dagger minute ventilation) correlated with the pH-slope (a dagger pH/a dagger oxygen uptake) (r = -0.616, p &lt; 0.0001; r = -0.629, p &lt; 0.0001, respectively, before and after the break point) and with the NE-slope (a dagger NE/a dagger oxygen uptake) (r = 0.443, p = 0.0012; r = 0.643, p &lt; 0.0001, respectively, before and after the break point). In TBsq patients during exercise, increases in circulating NE levels and exertional acidosis were correlated with exertional dyspnea.

    DOI: 10.1007/s12576-009-0083-1

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  • Transient role of CD4(+)CD25(+) regulatory T cells in mycobacterial infection in mice 査読

    Yuriko Ozeki, Isamu Sugawara, Tadashi Udagawa, Toshiaki Aoki, Mayuko Osada-Oka, Yoshitaka Tateishi, Hajime Hisaeda, Yuji Nishiuchi, Nobuyuki Harada, Kazuo Kobayashi, Sohkichi Matsumoto

    INTERNATIONAL IMMUNOLOGY   22 ( 3 )   179 - 189   2010年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    CD4(+)CD25(+) regulatory T (Treg) cells cause immune suppression by inhibiting T cell effector functions and play pivotal roles not only in self-tolerance but also in immune response to parasitic microbial pathogens. Mycobacteria are major parasitic bacterial pathogens, but the role of CD4(+)CD25(+) Treg cells in mycobacterial infection is not yet defined. In this study we found that, at the early stage of infection, depletion of CD25(+) cells reduced both bacterial load and granuloma formation in mice infected with Mycobacterium tuberculosis strains, such as M. tuberculosis Erdman or M. tuberculosis Kurono. However, at a later stage of infection, bacterial burden and histopathology were similar regardless of depletion of CD25(+) cells. Severe combined immunodeficient (SCID) mice reconstituted with CD4(+)CD25(-) T cells alone or a combination of CD4(+)CD25(+) and CD4(+)CD25(-) T cells showed similar bacterial loads and survival kinetics after infection with M. tuberculosis Erdman. Consistent with in vivo data, in vitro studies revealed that mycobacterial antigens, purified protein derivative of tuberculin (PPD), failed to induce the suppressive function of CD4(+)CD25(+) Treg cells to CD4(+)CD25(-) effector T cells, as demonstrated by the lack of response of CD4(+)CD25(+) T cells to PPD, in mice chronically infected with Mycobacterium bovis bacillus Calmette-Guerin and M. tuberculosis. Our data show that CD4(+)CD25(+) Treg cells have a transient effect at the early stage of mycobacterial infection but, contrary to the expectation, have little impact on the overall course of infection.

    DOI: 10.1093/intimm/dxp126

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  • Transient role of CD4+CD25+ regulatory T cells in mycobacterial infection in mice.

    Ozeki Yuriko, Sugawara Isamu, Udagawa Tadashi, Aoki Toshiaki, Osada-Oka Mayuko, Tateishi Yoshitaka, Hisaeda Hajime, Nishiuchi Yuji, Harada Nobuyuki, Kobayashi Kazuo, Matsumoto Sohkichi

    Int Immunol   22 ( 3 )   179 - 189   2010年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    CD4(+)CD25(+) regulatory T (Treg) cells cause immune suppression by inhibiting T cell effector functions and play pivotal roles not only in self-tolerance but also in immune response to parasitic microbial pathogens. Mycobacteria are major parasitic bacterial pathogens, but the role of CD4(+)CD25(+) Treg cells in mycobacterial infection is not yet defined. In this study we found that, at the early stage of infection, depletion of CD25(+) cells reduced both bacterial load and granuloma formation in mice infected with Mycobacterium tuberculosis strains, such as M. tuberculosis Erdman or M. tuberculosis Kurono. However, at a later stage of infection, bacterial burden and histopathology were similar regardless of depletion of CD25(+) cells. Severe combined immunodeficient (SCID) mice reconstituted with CD4(+)CD25(-) T cells alone or a combination of CD4(+)CD25(+) and CD4(+)CD25(-) T cells showed similar bacterial loads and survival kinetics after infection with M. tuberculosis Erdman. Consistent with in vivo data, in vitro studies revealed that mycobacterial antigens, purified protein derivative of tuberculin (PPD), failed to induce the suppressive function of CD4(+)CD25(+) Treg ce

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  • 肺Mycobacterium avium complex症持続排菌 症例における薬剤感受性試験. 査読

    立石善隆, 元根正晴, 好村研二, 三木真理, 三木啓資, 北田清悟, 橋本尚子, 平賀通, 前倉亮治

    日本呼吸器学会雑誌.   48 ( 11 )   797 - 802   2010年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • Acidosis and raised norepinephrine levels are associated with exercise dyspnoea in idiopathic pulmonary fibrosis 査読

    Keisuke Miki, Ryoji Maekura, Toru Hiraga, Hisako Hashimoto, Seigo Kitada, Mari Miki, Kenji Yoshimura, Yoshitaka Tateishi, Kenji Fushitani, Masaharu Motone

    RESPIROLOGY   14 ( 7 )   1020 - 1026   2009年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Background and objective: Exertional dyspnoea limits patients with IPF in their activities of daily living. The mechanism, however, has not been elucidated. This study tested the hypothesis in IPF that exertional dyspnoea correlates with cardiopulmonary exercise responses, specifically changes in arterial blood pH and plasma norepinephrine (NE).
    Methods: Cardiopulmonary exercise testing with measurements of dyspnoea (Borg scale), plasma NE, plasma lactate and arterial blood gases were performed in 29 patients with IPF and in nine controls.
    Results: Both groups showed obvious break points in dyspnoea changes during exercise. In IPF, an abrupt change in the Borg scale, pH, PaCO(2) and plasma NE occurred in the late exercise phase after the &apos;break point&apos;. Compared with controls, patients with IPF had significantly higher HCO(3)(-) levels and physiologic dead space/tidal volume during exercise. In IPF, during both exercise phases, the dyspnoea slope (Delta Borg scale/Delta minute ventilation) correlated with the pH slope (Delta pH/Delta oxygen uptake) (before the break point: r = -0.537, P = 0.0022; r = -0.886, P &lt; 0.0001, after the break point) and the NE slope (Delta NE/Delta oxygen uptake) (before the break point: r = 0.481, P = 0.0075; R = 0.784, P &lt; 0.0001, after the break point).
    Conclusions: In patients with IPF, exercise-induced acidosis and increases in circulating NE levels were associated with intensity of exertional dyspnoea.

    DOI: 10.1111/j.1440-1843.2009.01607.x

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  • Mycobacterium avium Complex Organisms Predominantly Colonize in the Bathtub Inlets of Patients' Bathrooms 査読

    Yukiko Nishiuchi, Aki Tamaru, Seigo Kitada, Takahiro Taguri, Sohkichi Matsumoto, Yoshitaka Tateishi, Mamiko Yoshimura, Yuriko Ozeki, Narumi Matsumura, Hisashi Ogura, Ryoji Maekura

    JAPANESE JOURNAL OF INFECTIOUS DISEASES   62 ( 3 )   182 - 186   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL INST INFECTIOUS DISEASES  

    Medical treatment of pulmonary Mycobacterium avium complex (MAC) disease does not always provide curative effects and is frequently hampered by recurrence. This suggests the presence of a reservoir for MAC in the environment surrounding patients. We previously reported the recovery of MAC isolates from the residential bathrooms of outpatients. In the present study, to ascertain the colonizing sites and the possibility of an MAC reservoir in the bathrooms of patients, we tested the recovery and the genetic diversity of MAC isolates from 6 sites of specimens, including 2 additional sampling sites, inside the showerhead and the bathtub inlet, in the residential bathrooms of patients with pulmonary MAC disease. MAC isolates were recovered from 15 out of the 29 bathrooms (52%), including specimens from 14 bathtub inlets and 3 showerheads. Nearly half of these bathrooms (7/15) contained MAC strains that were identical or similar to their respective clinical isolates. Additionally, in 5 out of 15 bathrooms, polyclonal colonization was revealed by pulsed-field gel electrophoresis. The results imply that colonization of MAC organisms in the bathrooms of MAC patients occurs predominantly in the bathtub inlets, and there is thus a risk of infection and/or reinfection for patients via use of the bathtub and other sites in the bathroom.

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  • Virulence of Mycobacterium avium complex strains isolated from immunocompetent patients 査読

    Yoshitaka Tateishi, Yukio Hirayama, Yuriko Ozeki, Yukiko Nishiuchi, Mamiko Yoshimura, Jing Kang, Atsushi Shibata, Kazuto Hirata, Seigo Kitada, Ryoji Maekura, Hisashi Ogura, Kazuo Kobayashi, Sohkichi Matsumoto

    MICROBIAL PATHOGENESIS   46 ( 1 )   6 - 12   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD  

    Mycobacterium avium complex (MAC) disease has been increasing worldwide not only in immunocompromised but also in immunocompetent humans. However, the relationship between mycobacterial strain virulence and disease progression in immunocompetent humans is unclear. In this study, we isolated 6 strains from patients with pulmonary MAC disease. To explore the virulence, we examined the growth in human THP-1 macrophages and pathogenicity in C57BL/6 mice. We found that one strain, designated 198, which was isolated from a patient showing the most progressive disease, persisted in THP-1 cells. In addition, strain 198 grew to a high bacterial load with strong inflammation in mouse lungs and spleens 16 weeks after infection. To our knowledge, strain 198 is the first isolated MAC strain that exhibits hypervirulence consistently for the human patient, human macrophages in vitro, and even for immunocompetent mice. Other strains showed limited survival and weak virulence both in macrophages and in mice, uncorrelated to disease progression in human patients. We demonstrated that there is a hypervirulent clinical MAC strain whose experimental virulence corresponds to the serious disease progression in the patients. The existence of such strain suggests the involvement of bacterial virulence in the pathogenesis of pulmonary MAC disease in immunocompetent status. (c) 2008 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.micpath.2008.10.007

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  • Evaluation of muscle oxygenation during exercise by NIRS in normal subjects – Significance of the NIRS threshold. 査読

    Fujimoto S, Yoshikawa T, Tateishi Y, Wang L, Hara T, Mimura T, Nakao H, Hirata K

    J Jpn Coll Angiol.   47 ( 1 )   21 - 27   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Effects of tiotropium or combined therapy with salmeterol on hyperinflation in COPD. 査読

    Eguchi Y, Tateishi Y, Umeda N, Yoshikawa T, Kamoi H, Kanazawa H, Kudoh S, Hirata K, Fujimoto S

    Osaka City Med J.   53 ( 1 )   25 - 34.   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:大阪市立大学  

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  • 慢性閉塞性肺疾患の運動生理―筋内酸素動態を含めて―. 査読

    藤本繁夫, 吉川貴仁, 立石善隆, 平田一人

    日本臨床生理学会誌.   36   75 - 81   2006年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • 呼吸器診療における運動負荷試験. 査読

    平田一人, 立石善隆, 江口陽介

    呼吸.   25   522 - 529   2006年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • Evaluation of peripheral muscle oxygenation during exercise by spatially resolved spectroscopy in patients with chronic obstructive pulmonary disease 査読

    Yoshitaka Tateishi, Takahiro Yoshikawa, Hiroshi Kanazawa, Hiroshi Fujiwara, Kazuto Hirata, Junichi Yosnikawa, Shigeo Fujimoto

    Osaka City Medical Journal   51 ( 2 )   65 - 72   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background: Spatially resolved (SR) spectroscopy has enabled non-invasive and continuous measurement of muscle oxygen saturation during exercise. In patients with chronic obstructive pulmonary disease (COPD), skeletal muscle dysfunction has been widely studied histochemically and biochemically. However, impairment of muscle oxygenation during exercise has not been elucidated yet. Methods: We measured oxygen saturation in the vastus lateralis muscle (SmO 2) using SR spectrometry during incremental cycle exercise in 16 COPD patients and 10 age-matched healthy subjects. Results: Significant decrease in SmO2 was found at peak exercise compared with warm-up in both groups (56.9±6.0% to 47.3±6.8% in patients with COPD, p&lt
    0.001
    60.7±5.8% to 49.9±7.7% in healthy subjects, p&lt
    0.01). The decrease in SmO2 was linear with respect to increase in work rate, and the slope of SmO2 was significantly steeper in COPD patients than in healthy subjects (-0.282±0.159 vs -0.107±0.057 %/Watt, p&lt
    0.001). The slope of SmO2 in COPD patients significantly correlated with body mass index (BMI) (p&lt
    0.01), peak percutaneous oxygen saturation (p&lt
    0.05), and peak pulmonary oxygen consumption (p&lt
    0.05). Stepwise regression analysis revealed that BMI was a significant determinant of the SmO2 slope (p=0.01). Conclusions: We conclude that oxygenation of peripheral muscle is impaired during exercise in COPD patients and that BMI contributes independently to the change of muscle oxygen saturation with exercise in COPD patients. SR spectroscopy will provide useful information for the study of the dynamics of muscle oxygenation in COPD patients.

    Scopus

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  • 運動時の喚気応答. 査読

    藤本繁夫, 吉川貴仁, 立石善隆

    臨床運動療法会誌.   7   1 - 6   2005年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • [Grading of officially acknowledged respiratory disability and exercise disorders using cardiopulmonary exercise testing in patients with chronic respiratory diseases]. 査読

    Tateishi Y, Maekura R, Yoshimura K, Kitada S, Hirotani A, Okuda Y, Hiraga T, Ito M

    Nihon Kokyuki Gakkai zasshi = the journal of the Japanese Respiratory Society   42 ( 4 )   299 - 305   2004年4月

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  • 呼吸器疾患の身体障害者認定における障害程度等級と運動機能障害についての検討. 査読

    立石善隆, 前倉亮治, 好村研二, 北田清悟, 廣谷淳, 奥田好成, 平賀通, 伊藤正己

    日本呼吸器学会雑誌.   42 ( 4 )   299 - 305   2004年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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▶ 全件表示

書籍等出版物

  • 人獣共通感染症-改訂版.

    西内由紀子, 立石善隆, 松本壮吉( 担当: 共著)

    医薬ジャーナル社  2011年 

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    記述言語:日本語 著書種別:学術書

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  • 呼吸器疾患の運動療法と運動負荷テスト―改訂第2版

    立石 善隆, 平田 一人( 担当: 共著)

    克誠堂出版  2007年 

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    記述言語:日本語 著書種別:学術書

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MISC

  • 野生型結核菌抗原を用いた抗体検出による活動性結核の診断と発症予測法の検討

    山崎 智也, 石川 智史, 田村 敏生, 塚本 裕美子, Nyoman Desak, 吉田 豊, 尾関 百合子, 西山 晃史, 立石 善隆, 松本 壮吉

    日本細菌学雑誌   78 ( 1 )   69 - 69   2023年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 抗酸菌感染症 抗MBGL抗体を組み入れた肺MAC症予後予測スコアリングシステムの考案

    木田 博, 北田 清悟, 三木 啓資, 三木 真理, 立石 善隆, 松本 荘吉, 前倉 亮治

    日本呼吸器学会誌   11 ( 増刊 )   159 - 159   2022年4月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • マイコバクテリア由来新規Z、E混成型プレニル基還元酵素の機能解析

    阿部 透, 袴田 真理子, 西山 晃史, 立石 善隆, 松本 壮吉, 邊見 久, 上田 大次郎, 佐藤 努

    日本放線菌学会大会講演要旨集   35回   55 - 55   2021年9月

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    記述言語:日本語   出版者・発行元:日本放線菌学会  

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  • 低酸素環境と疾患(がん、感染症)の分子論 低酸素休眠抗酸菌の主要タンパク質Mycobacterial DNA-binding protein 1(My cobacterial DNA-binding protein 1, a major protein in hypoxic dormant mycobacteria)

    西山 晃史, 古寺 哲幸, 清水 将裕, Savitskaya Anna, Enany Shymaa, 真柳 浩太, 山口 雄大, 尾関 百合子, 立石 善隆, 松本 壮吉

    日本細菌学雑誌   76 ( 1 )   57 - 57   2021年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 病原因子と生態防御(感染モデル・寄生・免疫・ワクチン)/病原体と感染症 組織透明化/3次元イメージング「CUBIC」による抗酸菌感染の生体内モニタリング

    袴田 真理子, 井内 絵梨奈, 横山 晃, 尾関 百合子, 西山 晃史, 立石 善隆, 大橋 璃子, 菊地 利明, 田井中 一貴, 松本 壮吉

    日本細菌学雑誌   76 ( 1 )   59 - 59   2021年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 抗酸菌症治療薬を目指した標的蛋白質の発現と精製

    大原 由貴子, 小林 悠, 尾関 百合子, 西山 晃史, 立石 善隆, 奥田 修二郎, 神谷 重樹, 北所 健悟, 松本 壮吉

    日本細菌学雑誌   75 ( 1 )   74 - 74   2020年1月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • Existence of extracellular DNA in pathogenic mycobacteria and its role in mycobacterial physiology(和訳中)

    イリノフ・アレクサンドル, シャバン・アミナ, 袴田 真理子, 西山 晃史, 尾関 百合子, 福島 由華里, 中島 千絵, 立石 善隆, 鈴木 定彦, 松本 壮吉

    日本細菌学雑誌   75 ( 1 )   74 - 74   2020年1月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 抗酸菌ヒストン様タンパク質の天然変性領域依存的なDNA凝集作用

    西山 晃史, 成田 知恕, 古寺 哲幸, 小林 瑶子, 武藤 寛亨, 渡辺 順也, 大原 直也, 尾関 百合子, 立石 善隆, 松本 壮吉

    日本細菌学雑誌   75 ( 1 )   132 - 132   2020年1月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 菌の休眠と覚醒のメカニズムと意義 休眠中のマイコバクテリアの主要タンパク質であるマイコバクテリアのヒストン様タンパク質MDP1の機能(The functions of mycobacterial histone-like protein MDP1, a major protein in dormant mycobacteria)

    西山 晃史, Savitskaya Anna, 山口 雄大, 大原 直也, 成田 知恕, 古寺 哲幸, 尾関 百合子, 立石 善隆, 松本 壮吉

    日本細菌学雑誌   74 ( 1 )   8 - 8   2019年3月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 病原性抗酸菌はヒト赤血球にin vitroで感染する

    西内 由紀子, 立石 善隆, 北田 清悟, 尾関 百合子, 前倉 亮治, 松本 壮吉

    結核   93 ( 4 )   285 - 285   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本結核病学会  

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  • 2016年、キュウリを原因とした腸管出血性大腸菌O157の集団感染

    尾鶴 亮, 立石 善隆, 小西 典子, 松本 壮吉, 松葉 隆司, 藤井 潤

    日本細菌学雑誌   73 ( 1 )   68 - 68   2018年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 病原性抗酸菌はヒト赤血球にin vitroで感染する(Pathogenic mycobacteria infect human erythrocytes in vitro)

    西内 由紀子, 立石 善隆, 尾関 百合子, 山口 雄大, 松本 壮吉

    日本細菌学雑誌   73 ( 1 )   41 - 41   2018年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 潜在期結核菌抗原の精製と感染診断への応用

    大原 由貴子, 尾関 百合子, 立石 善隆, 西山 晃史, 山本 三郎, 中川 一路, 松本 壮吉

    日本細菌学雑誌   73 ( 1 )   64 - 64   2018年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 2016年、キュウリを原因とした腸管出血性大腸菌O157の集団感染

    尾鶴 亮, 立石 善隆, 小西 典子, 松本 壮吉, 松葉 隆司, 藤井 潤

    日本細菌学雑誌   73 ( 1 )   68 - 68   2018年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • メチシリン耐性黄色ブドウ球菌に対するヤスダヨーグルトの抗菌活性の検討

    田島 陽介, 立石 善隆, 亀山 仁史, 松本 壮吉, 若井 俊文

    新潟医学会雑誌   131 ( 11 )   645 - 653   2017年11月

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    記述言語:日本語   出版者・発行元:新潟医学会  

    【目的】新潟県で開発・市販されているヤスダヨーグルト(以下YYG)は、メチシリン耐性黄色ブドウ球菌(methicillin-resistant Staphylococcus aureus;以下MRSA)に対して抗菌活性を示す。本研究の目的は、YYGのMRSAに対する抗菌活性成分を同定することである。【方法】1)抗菌活性の評価:ディスク拡散法により抗菌活性を検討した。2)YYGの遠心分離成分の抗菌活性の評価:YYGを4000rpm、15分の条件で遠心分離し、得られた上清および沈澱物成分について抗菌活性を評価した。3)抗菌活性を示す乳酸菌のYYGからの単離・同定:MRSA菌液を添加したMRS寒天培地にYYGを塗布し、37℃、24時間の好気培養を行った。阻止円を形成したコロニーを採取し、MRS液体培地を用いて24時間静置培養(37℃、好気条件)した。16S rRNA遺伝子解析を行い、抗菌活性を示す乳酸菌種を同定した。4)乳酸菌が産生する抗菌活性成分の同定:抗菌活性を示す乳酸菌培養液上清に対して加熱・限外濾過・アルカリ処理・消化酵素処理の各処理を行い、抗菌活性の変化を評価した。その結果から、抗菌活性成分の同定を試みた。5)培養条件が乳酸菌増殖に与える影響の検討:好気培養と嫌気培養、および静置培養と振盪培養による乳酸菌培養を行い、各培養条件と乳酸菌増殖、培養液上清pHおよび抗菌活性との関連をMann-WhitneyのU検定で解析した。また、培養液上清pHと抗菌活性の強さを検討した。【結果】1)抗菌活性の評価:ディスク拡散法により適切に抗菌活性を半定量的に評価することが可能であった。2)YYGの遠心分離成分の抗菌活性の評価:YYGの遠心分離成分のうち、沈澱物に強い抗菌活性を認めた。また、YYGをビーズ破砕した後に遠心分離すると、沈澱物の抗菌活性は消失した。よって、抗菌活性成分はYYGに含まれる乳酸菌が産生していると推測した。3)抗菌活性を示す乳酸菌のYYGからの分離・同定:16S rRNA遺伝子解析の結果、Lactobacillus delbrueckii subsp.bulgaricus(以下LDB)がMRSAに対する抗菌活性を示すことが判明した。4)乳酸菌が産生する抗菌活性成分の同定:LDB上清はアルカリ処理により抗菌活性が減弱または消失した。さらに、過酸化水素を分解するカタラーゼをLDB培養液に添加すると、抗菌活性が著明に減弱した。5)各培養条件における乳酸菌培養液の比較:静置培養は振盪培養に比べて菌の増殖がよく(P=0.002)、また上清のpHが有意に低下し(P=0.002)、抗菌活性は強かった(P=0.002)。一方、好気培養と嫌気培養の間では、菌の増殖、上清pH、抗菌活性のいずれも有意な差を認めなかった(P=0.310、P=0.589、P=1.000)。また、LDB培養液上清pHと阻止円径との間に強い負の相関関係を認めた(相関係数=-0.875、P&lt;0.001)。【結論】YYGのMRSAに対する抗菌活性は、YYGに含まれる乳酸菌LDBが産生する乳酸および過酸化水素によるものであることが示唆された。(著者抄録)

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  • 潜在期結核菌抗原の精製と感染診断への応用(Significance of the histone-like protein with the native structure for diagnosis of asymptomatic tuberculosis)

    西田 由貴子, 尾関 百合子, 立石 善隆, 西山 晃史, 山本 三郎, 中川 一路, 松本 壮吉

    日本細菌学雑誌   72 ( 1 )   161 - 161   2017年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 非結核性抗酸菌のバイオフィルム形成における栄養条件とGlycopeptidolipidの役割

    西内 由紀子, 戸谷 孝洋, 立石 善隆, 金子 幸弘, 松本 壮吉

    日本細菌学雑誌   72 ( 1 )   81 - 81   2017年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 抗酸菌の長期の生存に必須な細胞機能のヒストン様タンパク質依存的な制御(Histone-like protein-dependent control of mycobacterial functions critical for long-term survival)

    西山 晃史, Enany Shymaa, 立石 善隆, 尾関 百合子, Savitskaya Anna G, 山口 雄大, 西田 由貴子, 阿戸 学, 松本 壮吉

    日本細菌学雑誌   72 ( 1 )   97 - 97   2017年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • Mycobacterium kyorinenseおよび近縁種Mycobacterium celatumの薬剤耐性関連遺伝子の解析

    大西 宏明, 米谷 正太, 大塚 弘毅, 荒木 光二, 松本 壮吉, 立石 善隆, 河合 伸, 渡邊 卓

    感染症学雑誌   91 ( 1 )   100 - 100   2017年1月

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    記述言語:日本語   出版者・発行元:(一社)日本感染症学会  

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  • ケニア共和国成人を対象としたBCGブースターワクチン候補抗原の適性検討

    尾関百合子, 濱野真二郎, 松本壮吉, 岡真優子, 立石善隆

    長崎大学熱帯医学研究拠点共同研究報告集   2015   25‐29   2016年8月

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    記述言語:日本語  

    J-GLOBAL

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  • MDP1によるマイコバクテリア代謝抑制(Mycobacterial metabolism repression by MDP1)

    Enany Shymaa, 西山 晃史, 立石 善隆, 松本 壮吉

    感染症学雑誌   90 ( 臨増 )   303 - 303   2016年3月

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    記述言語:英語   出版者・発行元:(一社)日本感染症学会  

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  • 非結核性抗酸菌のバイオフィルム形成における細胞壁糖脂質の役割

    西内 由紀子, 立石 善隆, 金子 幸弘, 松本 壮吉

    結核   91 ( 3 )   352 - 352   2016年3月

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    記述言語:日本語   出版者・発行元:(一社)日本結核病学会  

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  • 抗酸菌のタンパク質の抗原性と機能の分析(Analysis of antigenicity and functions of mycobacterial proteins)

    Enany Shymaa, 尾関 百合子, 西山 晃史, Savitskaya Anna, 立石 善隆, 阿戸 学, 山本 格, 松本 壮吉

    日本細菌学雑誌   71 ( 1 )   65 - 65   2016年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 結核菌におけるポリフェノールの抗菌作用の検討

    立石 善隆, 尾関 百合子, 西山 晃史, 松本 壮吉

    日本細菌学雑誌   71 ( 1 )   139 - 139   2016年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 潜在期結核菌抗原の精製と感染診断への応用

    西田 由貴子, 北所 健悟, 尾関 百合子, 立石 善隆, 井上 学, 仁木 満美子, 金子 幸弘, 松本 壮吉, 有坂 文雄, 森川 耿右, 津中 康央, 藤原 芳江, 片平 正人, 真嶋 司, 前倉 亮治

    日本細菌学雑誌   71 ( 1 )   105 - 105   2016年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 非結核性抗酸菌のバイオフィルム形成におけるGlycopeptidolipidの役割

    西内 由紀子, 戸谷 孝洋, 立石 善隆, 金子 幸弘, 松本 壮吉

    日本細菌学雑誌   71 ( 1 )   111 - 111   2016年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 結核・抗酸菌症に関する最近のprovocativeな研究 ルシフェラーゼ発現リコンビナントBCGによる新規結核薬の迅速スクリーニング系の確立と実践(Latest provocative studies on tuberculosis and mycobacterial infections A new screen for TB drug candidates utilizing a luciferase-expressing recombinant BCG)

    尾関 百合子, 山口 雄大, Enany Shymaa, 五十嵐 雅之, 西内 由紀子, 岡 真優子, 岩本 朋忠, 小椋 義俊, 林 哲也, 立石 善隆, 西山 晃史, 松本 壮吉

    日本細菌学雑誌   71 ( 1 )   40 - 40   2016年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 結核菌の病原因子 招待

    立石 善隆, 松本 壮吉

    呼吸器内科   29 ( 1 )   65 - 70   2016年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)   出版者・発行元:科学評論社  

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    その他リンク: http://search.jamas.or.jp/link/ui/2016139494

  • プロバイオティクス医療を視野に入れたヨーグルトの抗菌効果の検討

    田島 陽介, 岡部 康之, 立石 善隆, 西山 晃史, 尾関 百合子, 亀山 仁史, 松本 壮吉, 若井 俊文

    新潟医学会雑誌   129 ( 10 )   593 - 600   2015年10月

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    記述言語:日本語   出版者・発行元:新潟医学会  

    【目的】消化管手術の周術期には、黄色ブドウ球菌などの皮膚常在菌による創部感染、絶食による腸内細菌叢の攪乱に伴うBacterial translocation、抗菌薬使用に伴うmethicillin-resistant Staphylococcus aureus(MRSA)などの薬剤耐性菌の出現が起こりうる。このような状況に対して応用的プロバイオティクスによる周術期感染制御を視野に、ヨーグルト製品のもつ抗菌効果の特性を微生物学的に解析した。【方法】新潟県で開発され市販されているヤスダヨーグルト(以下、YYG)に注目して、1)抗菌活性スペクトル(被験株:methicillin-sensitive Staphylococcus aureus(MSSA)ならびにMRSA臨床分離株、大腸菌株DH5α)、2)配合菌株の異なる他の5種の市販ヨーグルト製品との抗菌活性の相違、3)YYG各配合菌種(Yc-x11およびYc-180)の示す抗菌作用、および4)抗菌活性を示す分離画分について、ディスク法による阻止円形成により検討した。【結果】YYGは大腸菌に比してMSSAに優位な抗菌活性を示した。また、YYGはMRSA臨床分離株に対しても抗菌活性を示した。他製品との比較において、YYGは最も高い抗菌活性を示した。YYG配合菌種は、通常の低塩濃度培地上では抗菌活性を示したものの高塩濃度条件下では、その効果は減弱あるいは消失した。YYGの抗菌活性は、上清ではなく沈殿画分に認めた。【結論】YYGはMRSAを含む黄色ブドウ球菌に対する直接的な抗菌活性を示した。その抗菌効果は、特有の配合菌種に依拠する抗菌活性物質によることが示唆された。ヨーグルトの中でもYYGは極めて有望な抗菌性機能食品として、周術期感染制御に貢献できる可能性がある。(著者抄録)

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  • 潜在性結核のバイオマーカーとしての抗Antigen85およびMycobacteri DNA‐binding protein1抗体

    岡真優子, 立石善隆, 平山幸雄, 尾関百合子, 前倉亮次, 小林和夫, 松本壮吉

    日本熱帯医学会大会プログラム抄録集   54th   130   2013年9月

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    記述言語:日本語  

    J-GLOBAL

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  • 非結核性抗酸菌が形成するバイオフィルムの生態学的特徴

    西内 由紀子, 戸谷 孝洋, 立石 善隆, 松本 壮吉

    BACTERIAL ADHERENCE & BIOFILM   26   37 - 40   2013年5月

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    記述言語:日本語   出版者・発行元:日本バイオフィルム学会  

    非結核性抗酸菌は世界中の土壌や水系の環境中にバイオフィルムを形成して広く分布しており、ヒトに日和見感染する。なかでもMycobacterium avium、Mycobacterium intracellulareによる肺感染症は近年世界中で増加している。不思議な事にヒト-ヒト感染の報告はないので環境から生成したエアロゾルを吸入して感染している。私たちはM.avium、M.intracellulareが家庭の浴室に生息しており浴室から感染する危険性を報告してきた。そこで環境中のM.aviumが形成するバイオフィルムの生態が感染性およびエアロゾル形成に関与していると考え、その生態を走査型電子顕微鏡で観察した。本研究では抗酸菌のバイオフィルムの細胞外マトリックスの構成成分として知られているglycopeptidolipidの欠損株を用いて生態を比較した。その結果、M.aviumは膜に覆われたバイオフィルムを構築した。その膜の下に小型の桿菌がぎっしり詰まっていた。その桿菌には細胞間マトリックスがほとんど認められなかった。glycopeptidolipid欠損株には、菌を覆う膜の形成は認められなかった。M.aviumが形成するバイオフィルムの生態学的特徴から、菌体は膜状バイオフィルムに守られて、膜の内側で増殖していることが示唆された。同時に細胞間マトリックスがないので膜の一部が破綻すると内側の菌が個々に飛び出しエアロゾル形成に寄与すると思われる。(著者抄録)

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  • リコンビナントBCG(rBCG)を用いた迅速スクリーニング法による抗結核薬の探索と同定

    尾関 百合子, 西内 由紀子, 小椋 義俊, 岩本 朋忠, 林 哲也, 岡 真優子, 仁木 満美子, 立石 善隆, 平山 幸雄, 松本 壮吉

    日本細菌学雑誌   68 ( 1 )   181 - 181   2013年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 環境分離抗酸菌のバイオフィルム形成因子の検討

    戸谷 孝洋, 西内 由紀子, 立石 善隆, 松本 壮吉

    日本細菌学雑誌   68 ( 1 )   135 - 135   2013年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 潜在性結核患者の血清中での結核菌抗原に対するイムノグロブリンg(Antigen 85A and Mycobacterial DNA-binding protein 1 are targets of IgG in past tuberculosis)

    岡 真優子, 立石 善隆, 平山 幸雄, 尾関 百合子, 小林 和夫, 松本 壮吉

    日本細菌学雑誌   68 ( 1 )   214 - 214   2013年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 高病原性非結核性抗酸菌臨床菌株のゲノムシーケンス(Draft Genome Sequence of A Hypervirulent Clinical Mycobacterium intracellulare Strain)

    立石 善隆, 平山 幸雄, 尾関 百合子, 西内 由紀子, 仁木 満美子, 小椋 義俊, 林 哲也, 小林 和夫, 松本 壮吉

    日本細菌学雑誌   68 ( 1 )   176 - 176   2013年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 運動負荷心肺機能検査を用いたCOPDの予後因子の検討

    前倉 亮治, 三木 啓資, 北田 清悟, 好村 研二, 立石 善隆, 平賀 通

    日本呼吸ケア・リハビリテーション学会誌   22 ( Suppl. )   180s - 180s   2012年10月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸ケア・リハビリテーション学会  

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  • 高度運動耐応能低下のあるCOPD患者の動的病態

    前倉 亮治, 三木 啓資, 北田 清悟, 好村 研二, 立石 善隆, 平賀 通

    日本呼吸ケア・リハビリテーション学会誌   22 ( Suppl. )   124s - 124s   2012年10月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸ケア・リハビリテーション学会  

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  • 細菌付着の制御とBiofilm形成 非結核性抗酸菌が形成するバイオフィルムの生態学的特徴

    西内 由紀子, 戸谷 孝洋, 立石 善隆, 前倉 亮治, 松本 壮吉

    Bacterial Adherence & Biofilm 学術集会   26回   25 - 25   2012年7月

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    記述言語:日本語   出版者・発行元:日本バイオフィルム学会  

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  • COPD患者の労作時息切れに関連する運動誘発性アシドーシス

    三木 啓資, 玄山 宗到, 松浦 邦臣, 立石 善隆, 藤川 健弥, 好村 研二, 北田 清悟, 三木 真理, 橋本 尚子, 森 雅秀, 前倉 亮治

    日本呼吸器学会誌   1 ( 増刊 )   133 - 133   2012年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 肺MAC症患者に対するエリスロマイシン単剤投与の安全性と有効性

    松浦 邦臣, 北田 清悟, 玄山 宗到, 各務 慎一, 立石 善隆, 好村 研二, 三木 啓資, 三木 真理, 橋本 尚子, 森 雅秀, 前倉 亮治

    日本呼吸器学会誌   1 ( 増刊 )   149 - 149   2012年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • COPD患者における最大運動時の呼吸困難感と下肢筋内酸素化障害との関係

    立石 善隆, 好村 研二, 三木 真理, 三木 啓資, 北田 清悟, 前倉 亮治, 江口 陽介, 平田 一人, 藤本 繁夫

    日本呼吸器学会誌   1 ( 増刊 )   361 - 361   2012年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 当院における新規同定抗酸菌菌種の推移

    玄山 宗到, 北田 清悟, 立石 善隆, 松浦 邦臣, 好村 研二, 三木 啓資, 三木 真理, 森 雅秀, 前倉 亮治

    日本呼吸器学会誌   1 ( 増刊 )   363 - 363   2012年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • Mycobacterial DNA-binding protein 1(MDP1)に見いだされたFerritin-super family蛋白質様活性

    松本 壮吉, 岡 真優子, 西内 由紀子, 仁木 満美子, 尾関 百合子, 立石 善隆, 小林 和夫, 高塚 正樹, 佐藤 英介, 井上 正康

    日本細菌学雑誌   67 ( 1 )   149 - 149   2012年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 著しく運動耐応能が低下したCOPD患者の動的病態の特徴

    前倉 亮治, 三木 啓資, 北田 清悟, 立石 善隆, 好村 研二, 森 雅秀

    日本内科学会雑誌   101 ( Suppl. )   194 - 194   2012年2月

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    記述言語:日本語   出版者・発行元:(一社)日本内科学会  

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  • マウスモデルにおけるBCGワクチン効果の減衰に関する研究(Loss of anti-mycobacterial efficacy in mice over time following vaccination with BCG)

    尾関 百合子, 平山 幸雄, 岡 真優子, 立石 善隆, 瀧井 猛将, 山本 三郎, 小林 和夫, 松本 壮吉

    日本細菌学雑誌   67 ( 1 )   158 - 158   2012年2月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 高病原性Mycobacterium intracellulare臨床分離株のドラフトゲノム解析

    立石 善隆, 小椋 義俊, 平山 幸雄, 尾関 百合子, 西内 由紀子, 仁木 満美子, 林 哲也, 小林 和夫, 松本 壮吉, 王 亜軍, 北田 清悟, 前倉 亮治

    日本細菌学雑誌   67 ( 1 )   119 - 119   2012年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 閉塞性睡眠時無呼吸症候群患者の微量アルブミン尿に影響を与える因子の検討

    元根 正晴, 平賀 通, 北田 清悟, 好村 研二, 三木 啓資, 三木 真理, 立石 善隆, 前倉 亮治, 楽木 宏実

    日本老年医学会雑誌   48 ( 4 )   411 - 411   2011年7月

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    記述言語:日本語   出版者・発行元:(一社)日本老年医学会  

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  • 環境から分離したMycobacterium aviumのバイオフィルム形成

    西内 由紀子, 松本 壮吉, 立石 善隆, 北田 清悟, 前倉 亮治

    結核   86 ( 3 )   374 - 374   2011年3月

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    記述言語:日本語   出版者・発行元:(一社)日本結核病学会  

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  • 潜在性結核感染の診断におけるQFT、抗酸菌血清診断の有用性

    北田 清悟, 好村 研二, 立石 善隆, 三木 啓資, 三木 真理, 平賀 通, 森 雅秀, 前倉 亮治

    日本呼吸器学会雑誌   49 ( 増刊 )   169 - 169   2011年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • COPD患者における呼増運動負荷中の筋内脱酸素化動態の検討

    立石 善隆, 好村 研二, 橋本 尚子, 三木 真理, 三木 啓資, 北田 清悟, 平賀 通, 前倉 亮治, 江口 陽介, 平田 一人, 吉川 貴仁, 藤本 繁夫

    日本呼吸器学会雑誌   49 ( 増刊 )   188 - 188   2011年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • COPD グレリン投与によるCOPD治療 多施設無作為化二重盲検比較試験

    三木 啓資, 前倉 亮治, 立石 善隆, 好村 研二, 北田 清悟, 三木 真理, 橋本 尚子, 平賀 通, 吉川 雅則, 木村 弘, 有村 保次, 松元 信弘, 中里 雅光, 山原 研一, 永谷 憲歳, 寒川 賢治

    日本呼吸器学会雑誌   49 ( 増刊 )   119 - 119   2011年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 抗酸菌検査の進歩 結核血清診断の進歩

    立石 善隆, 北田 清悟, 松本 壮吉, 前倉 亮治

    結核   86 ( 3 )   310 - 310   2011年3月

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    記述言語:日本語   出版者・発行元:(一社)日本結核病学会  

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  • 生活環境における非結核性抗酸菌の分布. 招待 査読

    西内 由紀子, 立石 善隆, 松本 壮吉

    化学療法の領域   2011年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • COPDに対するグレリンと呼吸リハビリテーションによる併用療法の効果

    三木 啓資, 元根 正晴, 各務 慎一, 上浪 健, 立石 善隆, 好村 研二, 北田 清悟, 三木 真理, 橋本 尚子, 平賀 通, 前倉 亮治, 寒川 賢治

    日本呼吸器学会雑誌   48 ( 増刊 )   237 - 237   2010年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 長期にわたり経過観察し得た肺MAC症の臨床的検討

    上浪 健, 北田 清悟, 好村 研二, 各務 慎一, 立石 善隆, 元根 正晴, 三木 真理, 三木 啓資, 平賀 通, 前倉 亮治

    日本呼吸器学会雑誌   48 ( 増刊 )   211 - 211   2010年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 閉塞性睡眠時無呼吸症候群患者においてCOPD合併患者と他の呼吸器疾患を合併した患者との比較検討

    元根 正晴, 平賀 通, 北田 清悟, 三木 啓資, 三木 真理, 好村 研二, 立石 善隆, 橋本 尚子, 前倉 亮治

    日本呼吸器学会雑誌   48 ( 増刊 )   301 - 301   2010年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 潜在性結核の診断法の確立に向けた臨床への橋渡し研究(第1報)

    岡 真優子, 平山 幸雄, 立石 善隆, 小林 和夫, 松本 壮吉

    日本細菌学雑誌   65 ( 1 )   198 - 198   2010年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 病原性の起源を生態系の視点から考える 自然環境生息菌をモデルとして 生活環境由来Mycobacterium avium complexの遺伝子多型解析

    西内 由紀子, 松本 壮吉, 立石 善隆

    日本細菌学雑誌   65 ( 1 )   91 - 91   2010年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 肺MAC症持続排菌症例におけるRBTを含めた薬剤感受性の検討

    立石善隆, 菅野哲平, 北田清悟, 前倉亮治

    結核   84 ( 5 )   421   2009年5月

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    記述言語:日本語  

    J-GLOBAL

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  • 肺Mycobacteirum avium complex症患者由来M.aviumと豚由来M.aviumの血清型比較

    西内由紀子, 田栗貴博, 松本壮吉, 立石善隆, 北田清悟, 田丸亜貴, 鈴木定彦, 前倉亮治

    結核   84 ( 5 )   409   2009年5月

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    記述言語:日本語  

    J-GLOBAL

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  • QFT,TBGL,LAMを用いた結核菌感染症の検討

    菅野哲平, 立石善隆, 北田清吾, 前倉亮治

    結核   84 ( 5 )   377   2009年5月

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    記述言語:日本語  

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  • 肺結核後遺症患者の労作時息切れに関連する運動誘発性アシドーシスと血漿ノルエピネフリン変化

    三木啓資, 元根正晴, 菅野哲平, 伏谷建二, 立石善隆, 好村研二, 北田清悟, 三木真理, 平賀通, 前倉亮治

    日本呼吸器学会雑誌   47 ( 増刊 )   297 - 297   2009年5月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

    J-GLOBAL

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  • 閉塞性睡眠時無呼吸症候群患者における慢性炎症に影響を与える因子の検討

    元根 正晴, 平賀 通, 北田 清悟, 好村 研二, 三木 啓資, 三木 真理, 橋本 尚子, 立石 善隆, 前倉 亮治

    日本呼吸器学会雑誌   47 ( 増刊 )   329 - 329   2009年5月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 生活環境に分布するMycobacterium avium complexと臨床分離株の多型解析

    西内由紀子, 松本壮吉, 立石善隆, 鈴木定彦

    日本細菌学雑誌   64 ( 1 )   96   2009年2月

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    記述言語:日本語  

    J-GLOBAL

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  • 抗酸菌感染症研究における新たな視点 急速な臨床経過に合致した高病原性Mycobacterium avium-intracellulare complex菌株の同定

    立石 善隆, 平山 幸雄, 尾関 百合子, 西内 由紀子, 吉村 満美子, 小林 和夫, 松本 壮吉, 北田 清悟, 前倉 亮治

    日本細菌学雑誌   64 ( 1 )   93 - 93   2009年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 特発性肺線維症患者における、運動時の血漿ノルエピネフリン変化と息切れとの関連

    三木 啓資, 元根 正晴, 伏谷 建二, 立石 善隆, 藤川 健弥, 好村 研二, 北田 清悟, 三木 真理, 橋本 尚子, 平賀 通, 前倉 亮治

    日本呼吸器学会雑誌   46 ( 増刊 )   116 - 116   2008年5月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 当院における間質性肺疾患の臨床的検討

    北田 清悟, 好村 研二, 森 雅秀, 伏谷 建二, 立石 善隆, 三木 啓資, 三木 真理, 元根 正晴, 平賀 通, 前倉 亮治

    日本呼吸器学会雑誌   46 ( 増刊 )   271 - 271   2008年5月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 当院での過去5年間における人工呼吸器管理を施行した急性呼吸不全症例に対する検討

    伏谷 建二, 北田 清悟, 立石 善隆, 元根 正晴, 好村 研二, 三木 啓資, 三木 真理, 中 宣敬, 平賀 通, 前倉 亮治

    日本呼吸器学会雑誌   46 ( 増刊 )   338 - 338   2008年5月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • 肺Mycobacterium avium complex(MAC)症患者の家庭浴室に常在するMACと喀痰分離株の多型解析

    西内 由紀子, 田丸 亜貴, 北田 清悟, 田栗 貴博, 松本 壮吉, 立石 善隆, 前倉 亮治

    結核   83 ( 3 )   289 - 289   2008年3月

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    記述言語:日本語   出版者・発行元:(一社)日本結核病学会  

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  • 家庭浴室由来Mycobacterium avium complexの多型性と臨床分離株との相同性

    西内 由紀子, 松本 壮吉, 立石 善隆, 田丸 亜貴, 前倉 亮治, 北田 清悟, 田栗 貴博

    日本細菌学雑誌   63 ( 1 )   163 - 163   2008年2月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 肺線維症と肺気腫合併症例の臨床的検討

    伏谷 建二, 北田 清悟, 岩崎 剛雄, 元根 正晴, 立石 善隆, 好村 研二, 三木 真理, 三木 啓資, 中 宣敬, 平賀 通, 前倉 亮治

    日本呼吸器学会雑誌   45 ( 増刊 )   257 - 257   2007年4月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • COPD患者における、運動誘発低酸素血症の程度と運動時肺高血圧との関連

    三木 啓資, 平賀 通, 橋本 尚子, 北田 清悟, 三木 真理, 好村 研二, 中 宣敬, 元根 正晴, 立石 善隆, 岩崎 剛雄, 伏谷 建二, 藤川 健弥, 前倉 亮治

    日本呼吸器学会雑誌   45 ( 増刊 )   185 - 185   2007年4月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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▶ 全件表示

共同研究・競争的資金等の研究

  • 1. 近年急増中の虚弱中高齢肺MAC症患者に対する運動療法効果の基礎的研究

    2012年4月 - 2015年3月

    制度名:科学研究費助成事業

    研究種目:若手研究(B)

    提供機関:日本学術振興会

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    資金種別:競争的資金

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担当経験のある授業科目

  • 生体防御と感染(細菌学)

    2024年
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  • 生体防御と感染(総合)

    2024年
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    機関名:新潟大学