2021/10/20 更新

写真a

カワムラ メイコ
川村 名子
KAWAMURA Meiko
所属
脳研究所 生命科学リソース研究センター 特任助教
職名
特任助教
外部リンク

学位

  • 博士(医学) ( 1995年3月   京都大学 )

研究キーワード

  • 神経化学

  • ウイルス学

  • 感染免疫学

  • 分子生物学

研究分野

  • ライフサイエンス / 医化学

経歴(researchmap)

  • 新潟大学   脳研究所 基礎神経科学部門   特任助教

    2015年4月 - 現在

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  • 新潟青陵大学   非常勤講師

    2001年4月 - 現在

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経歴

  • 新潟大学   脳研究所 基礎神経科学部門   特任助教

    2015年4月 - 現在

学歴

  • 北海道大学   獣医学研究科   予防治療学

    1983年4月 - 1985年3月

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    国名: 日本国

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  • 北海道大学   獣医学部   獣医学科、予防治療学専攻

    1979年4月 - 1983年3月

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    国名: 日本国

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所属学協会

取得資格

  • 獣医師

 

論文

  • Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain. 査読 国際誌

    Chihiro Nakamoto, Kohtarou Konno, Taisuke Miyazaki, Ena Nakatsukasa, Rie Natsume, Manabu Abe, Meiko Kawamura, Yugo Fukazawa, Ryuichi Shigemoto, Miwako Yamasaki, Kenji Sakimura, Masahiko Watanabe

    The Journal of comparative neurology   528 ( 6 )   1003 - 1027   2020年4月

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    記述言語:英語  

    In the cerebellum, GluD2 is exclusively expressed in Purkinje cells, where it regulates synapse formation and regeneration, synaptic plasticity, and motor learning. Delayed cognitive development in humans with GluD2 gene mutations suggests extracerebellar functions of GluD2. However, extracerebellar expression of GluD2 and its relationship with that of GluD1 are poorly understood. GluD2 mRNA and protein were widely detected, with relatively high levels observed in the olfactory glomerular layer, medial prefrontal cortex, cingulate cortex, retrosplenial granular cortex, olfactory tubercle, subiculum, striatum, lateral septum, anterodorsal thalamic nucleus, and arcuate hypothalamic nucleus. These regions were also enriched for GluD1, and many individual neurons coexpressed the two GluDs. In the retrosplenial granular cortex, GluD1 and GluD2 were selectively expressed at PSD-95-expressing glutamatergic synapses, and their coexpression on the same synapses was shown by SDS-digested freeze-fracture replica labeling. Biochemically, GluD1 and GluD2 formed coimmunoprecipitable complex formation in HEK293T cells and in the cerebral cortex and hippocampus. We further estimated the relative protein amount by quantitative immunoblotting using GluA2/GluD2 and GluA2/GluD1 chimeric proteins as standards for titration of GluD1 and GluD2 antibodies. Intriguingly, the relative amount of GluD2 was almost comparable to that of GluD1 in the postsynaptic density fraction prepared from the cerebral cortex and hippocampus. In contrast, GluD2 was overwhelmingly predominant in the cerebellum. Thus, we have determined the relative extracerebellar expression of GluD1 and GluD2 at regional, neuronal, and synaptic levels. These data provide a molecular-anatomical basis for possible competitive and cooperative interactions of GluD family members at synapses in various brain regions.

    DOI: 10.1002/cne.24792

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  • GluD1 knockout mice with a pure C57BL/6N background show impaired fear memory, social interaction, and enhanced depressive-like behavior. 査読 国際誌

    Chihiro Nakamoto, Meiko Kawamura, Ena Nakatsukasa, Rie Natsume, Keizo Takao, Masahiko Watanabe, Manabu Abe, Tomonori Takeuchi, Kenji Sakimura

    PloS one   15 ( 2 )   e0229288   2020年

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    記述言語:英語  

    The GluD1 gene is associated with susceptibility for schizophrenia, autism, depression, and bipolar disorder. However, the function of GluD1 and how it is involved in these conditions remain elusive. In this study, we generated a Grid1 gene-knockout (GluD1-KO) mouse line with a pure C57BL/6N genetic background and performed several behavioral analyses. Compared to a control group, GluD1-KO mice showed no significant anxiety-related behavioral differences, evaluated using behavior in an open field, elevated plus maze, a light-dark transition test, the resident-intruder test of aggression and sensorimotor gating evaluated by the prepulse inhibition test. However, GluD1-KO mice showed (1) higher locomotor activity in the open field, (2) decreased sociability and social novelty preference in the three-chambered social interaction test, (3) impaired memory in contextual, but not cued fear conditioning tests, and (4) enhanced depressive-like behavior in a forced swim test. Pharmacological studies revealed that enhanced depressive-like behavior in GluD1-KO mice was restored by the serotonin reuptake inhibitors imipramine and fluoxetine, but not the norepinephrine transporter inhibitor desipramine. In addition, biochemical analysis revealed no significant difference in protein expression levels, such as other glutamate receptors in the synaptosome and postsynaptic densities prepared from the frontal cortex and the hippocampus. These results suggest that GluD1 plays critical roles in fear memory, sociability, and depressive-like behavior.

    DOI: 10.1371/journal.pone.0229288

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  • Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice. 査読 国際誌

    Srikanta Chowdhury, Chi Jung Hung, Shuntaro Izawa, Ayumu Inutsuka, Meiko Kawamura, Takashi Kawashima, Haruhiko Bito, Itaru Imayoshi, Manabu Abe, Kenji Sakimura, Akihiro Yamanaka

    eLife   8   2019年6月

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    記述言語:英語  

    Uninterrupted arousal is important for survival during threatening situations. Activation of orexin/hypocretin neurons is implicated in sustained arousal. However, orexin neurons produce and release orexin as well as several co-transmitters including dynorphin and glutamate. To disambiguate orexin-dependent and -independent physiological functions of orexin neurons, we generated a novel Orexin-flippase (Flp) knock-in mouse line. Crossing with Flp-reporter or Cre-expressing mice showed gene expression exclusively in orexin neurons. Histological studies confirmed that orexin was knock-out in homozygous mice. Orexin neurons without orexin showed altered electrophysiological properties, as well as received decreased glutamatergic inputs. Selective chemogenetic activation revealed that both orexin and co-transmitters functioned to increase wakefulness, however, orexin was indispensable to promote sustained arousal. Surprisingly, such activation increased the total time spent in cataplexy. Taken together, orexin is essential to maintain basic membrane properties and input-output computation of orexin neurons, as well as to exert awake-sustaining aptitude of orexin neurons.

    DOI: 10.7554/eLife.44927

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  • Autophosphorylation of F-actin binding domain of CaMKIIβ is required for fear learning. 査読 国際誌

    Kim K, Suzuki A, Kojima H, Kawamura M, Miya K, Abe M, Yamada I, Furuse T, Wakana S, Sakimura K, Hayashi Y

    Neurobiology of learning and memory   157   86 - 95   2019年1月

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  • Higher visual responses in the temporal cortex of mice 査読

    Nishino K, Tsukano H, Hishida R, Abe M, Nakai J, Kawamura M, Aiba A, Sakimura K, Shibuki K

    Scientific reports   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Retrograde BDNF to TrkB signaling promotes synapse elimination in the developing cerebellum 査読

    Myeongjeong Choo, Taisuke Miyazaki, Maya Yamazaki, Meiko Kawamura, Takanobu Nakazawa, Jianling Zhang, Asami Tanimura, Naofumi Uesaka, Masahiko Watanabe, Kenji Sakimura, Masanobu Kano

    NATURE COMMUNICATIONS   8 ( 195 )   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Elimination of early-formed redundant synapses during postnatal development is essential for functional neural circuit formation. Purkinje cells (PCs) in the neonatal cerebellum are innervated by multiple climbing fibers (CFs). A single CF is strengthened whereas the other CFs are eliminated in each PC dependent on postsynaptic activity in PC, but the underlying mechanisms are largely unknown. Here, we report that brain-derived neurotrophic factor (BDNF) from PC facilitates CF synapse elimination. By PC-specific deletion of BDNF combined with knockdown of BDNF receptors in CF, we show that BDNF acts retrogradely on TrkB in CFs, and facilitates elimination of CF synapses from PC somata during the third postnatal week. We also show that BDNF shares signaling pathway with metabotropic glutamate receptor 1, a key molecule that triggers a canonical pathway for CF synapse elimination. These results indicate that unlike other synapses, BDNF mediates punishment signal for synapse elimination in the developing cerebellum.

    DOI: 10.1038/s41467-017-00260-w

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  • The cellular and behavioral consequences of interleukin-1 alpha penetration through the blood-brain barrier of neonatal rats: A critical period for efficacy 査読

    M. Tohmi, N. Tsuda, Y. Zheng, M. Mizuno, H. Sotoyama, M. Shibuya, M. Kawamura, A. Kakita, H. Takahashi, H. Nawa

    NEUROSCIENCE   150 ( 1 )   234 - 250   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Proinflammatory cytokines circulating in the periphery of early postnatal animals exert marked influences on their subsequent cognitive and behavioral traits and are therefore implicated in developmental psychiatric diseases such as schizophrenia. Here we examined the relationship between the permeability of the blood-brain barrier to interleukin-1 alpha (IL-1 alpha) in neonatal and juvenile rats and their later behavioral performance. Following s.c. injection of IL-1 alpha into rat neonates, IL-1 alpha immunoreactivity was first detected in the choroid plexus, brain microvessels, and olfactory cortex, and later diffused to many brain regions such as neocortex and hippocampus. In agreement, IL-1 alpha administration to the periphery resulted in a marked increase in brain IL-1 alpha content of neonates. Repeatedly injecting IL-1 alpha to neonates triggered astrocyte proliferation and microglial activation, followed by behavioral abnormalities in startle response and putative prepulse inhibition at the adult stage. Analysis of covariance with a covariate of startle amplitude suggested that IL-1 alpha administration may influence prepulse inhibition. However, adult rats treated with IL-1 alpha as neonates exhibited normal learning ability as measured by contextual fear conditioning, two-way passive shock avoidance, and a radial maze task and had no apparent sign of structural abnormality in the brain. In comparison, when IL-1 alpha was administered to juveniles, the blood-brain barrier permeation was limited. The increases in brain IL-1 alpha content and immunoreactivity were less pronounced following IL-1 alpha administration and behavioral abnormalities were not manifested at the adult stage. During early development, therefore, circulating IL-1 alpha efficiently crosses the blood-brain barrier to induce inflammatory reactions in the brain and influences later behavioral traits. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.neuroscience.2007.08.034

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  • Association of 14-3-3ε gene hapoltype with completed suicide in Japanese. 査読

    Yanagi, M, Shirakawa, O, Kitamura, N, Okamura, K, Sakurai, K, Nishiguchi, N, Hashimoto, T, Nushida, H, Ueno, Y, Kanbe, D, Kawamura, M, Araki, K, Nawa, H, Maeda, K

    J. Hum. Genet.   50 ( 4 )   210 - 216   2005年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s10038-005-0241-0

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  • Brain-derived neurotrophic factor induces mammalian target of rapamycin-dependent local activation of translation machinery and protein synthesis in neuronal dendrites 査読

    Takei N, Inamura N, Kawamura M, Namba H, Hara K, Yonezawa K, Nawa H

    JOURNAL OF NEUROSCIENCE   24 ( 44 )   9760 - 9769   2004年11月

  • Prefrontal abnormality of schizophrenia revealed by DNA microarray: Impact on glial and neurotrophic gene expression 査読

    Tetsuji Sugai, Meiko Kawamura, Shuji Iritani, Kazuaki Araki, Takao Makifuchi, China Imai, Ryosuke Nakamura, Akiyoshi Kakita, Hitoshi Takahashi, Hiroyuki Nawa

    Annals of the New York Academy of Sciences   1025   84 - 91   2004年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:New York Academy of Sciences  

    DNA microarrays with isotope labeling from gene-specific primers enable sensitive detection of rare mRNAs, including neurotrophin and cytokine mRNAs in the brain. Using high-quality RNA from postmortem brains, gene-expression profiles covering 1373 genes were assessed in the dorsoprefrontal cortex of schizophrenic patients and compared with those of nonpsychiatric subjects. Statistical analysis of the DNA microarray data confirmed the findings of a previous GeneChip study by Hakak et al. (Proc. Natl. Acad. Sci. USA Vol. 98, pp. 4746-4751, 2001). The highest frequency of mRNA expression alterations occurred in oligodendrocyte- and astrocyte-related genes in the prefrontal cortex of schizophrenic patients, followed by the category for the genes for growth factors/neurotrophic factors and their receptors. Whether each mRNA signal represents the expression of the individual genes or homologous genes in the category remains to be determined, however. To control for potential medication effects on patients, RNA from cynomolgus monkeys that were treated with haloperidol for 3 months was also subjected to DNA microarray analysis. A few genes overlapped between the gene-expression profiles of the monkeys and patients. The present profiling study suggests a potential biological link between abnormal neurotrophic signals and impaired glial functions in schizophrenic pathology.

    DOI: 10.1196/annals.1316.011

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  • Prefrontal abnormality of schizophrenia revealed by DNA microarray - Impact on glial and neurotrophic gene expression 査読

    Sugai T, Kawamura M, Iritani S, Araki K, Makifuchi T, Imai C, Nakamura R, Kakita A, Takahashi H, Nawa H

    CURRENT STATUS OF DRUG DEPENDENCE / ABUSE STUDIES: CELLULAR AND MOLECULAR MECHANISMS OF DRUGS OF ABUSE AND NEUROTOXICITY   1025   84 - 91   2004年

  • A palmitoylated RING finger ubiquitin ligase and its homologue in the brain membranes 査読

    K Araki, M Kawamura, T Suzuki, N Matsuda, D Kanbe, K Ishii, T Ichikawa, T Kumanishi, T Chiba, K Tanaka, H Nawa

    JOURNAL OF NEUROCHEMISTRY   86 ( 3 )   749 - 762   2003年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING LTD  

    Ubiquitin (Ub) ligation is implicated in active protein metabolism and subcellular trafficking and its impairment is involved in various neurologic diseases. In rat brain, we identified two novel Ub ligases, Momo and Sakura, carrying double zinc finger motif and RING finger domain. Momo expression is enriched in the brain gray matter and testis, and Sakura expression is more widely detected in the brain white matter as well as in many peripheral organs. Both proteins associate with the cell membranes of neuronal and/or glial cells. We examined their Ub ligase activity in vivo and in vitro using viral expression vectors carrying myc-tagged Momo and Sakura. Overexpression of either Momo or Sakura in mixed cortical cultures increased total polyubiquitination levels. In vitro ubiquitination assay revealed that the combination of Momo and UbcH4 and H5c, or of Sakura and UbcH4, H5c and H6 is required for the reaction. Deletion mutagenesis suggested that the E3 Ub ligase activity of Momo and Sakura depended on their C-terminal domains containing RING finger structure, while their N-terminal domains influenced their membrane association. In agreement, Sakura associating with the membrane was specifically palmitoylated. Although the molecular targets of their Ub ligation remain to be identified, these findings imply a novel function of the palmitoylated E3 Ub ligase(s).

    DOI: 10.1046/j.1471-4159.2003.01875.x

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  • Brain-derived neurotrophic factor regulates surface expression of α-amino-3-hydroxy-5-methyle-4-isoxazoleproprionic acid receptors by enhancing the N-ethylmaleimide-sensitive factor/GluR2 interraction in developing neocortical neurons. 査読

    Narisawa-Saito, M, Iwakura, Y, Kawamura, M, Kazuaki, A, Kozaki, S, Takei, N, Nawa, H

    Journal of Biological Chemistry   277 ( 43 )   40901 - 40910   2002年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M202158200

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  • Brain-derived neurotrophic factor enhances neuronal translation by activating multiple initiation processes: comparison with the effects of insulin. 査読

    Takei N, Kawamura M, Hara K, Yonezawa K, Nawa H

    J Biol Chem.   276 ( 46 )   42818 - 42825   2001年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M103237200

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  • N-Methyl-D-aspartate-induced α-Amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) receptor down-regulation involves interaction of the carboxyl terminus of GluR2/3 with Pick1. 査読

    Iwakura, Y, Nagano, T, Kawamura, M, Horikawa, H, Ibaraki, K, Takei, N, Nawa, H

    Journal of Biological Chemistry   276 ( 43 )   40025 - 40032   2001年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M103125200

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  • Sindbis viral-mediated expression of Ca2+-permeable AMPA receptors at hippocampal CA1 synapses and induction of NMDA receptor-independent long-term potentiation 査読

    T Okada, N Yamada, W Kakegawa, K Tsuzuki, M Kawamura, H Nawa, M Iino, S Ozawa

    EUROPEAN JOURNAL OF NEUROSCIENCE   13 ( 8 )   1635 - 1643   2001年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL SCIENCE LTD  

    Gene manipulation in order to artificially express a particular gene in neurons in the central nervous system is a powerful tool for the analysis of brain function. Sindbis viral vectors have been developed to express high levels of foreign genes in postmitotic brain neurons with little transfection of glial cells. In this study, we expressed the gene encoding the unedited GluR2 (GluR-B) subunit of the AMPA-type glutamate receptor that forms inwardly rectifying and Ca2+-permeable channels, in rat CA1 hippocampal neurons in slice cultures using Sindbis viral vectors. The pyramidal cell layer of the CA1 region was injected with recombinant Sindbis viruses encoding both enhanced green fluorescent protein (GFP) and unedited GluR2. The GFP fluorescence from CA1 neurons could be detected as early as 6 h and reached a maximal level about 48 h postinfection. The inwardly rectifying and Ca2+-permeable AMPA receptors were expressed in most CA1 pyramidal cells expressing GFP. These AMPA receptors expressed by gene transfer were involved in fast excitatory neurotransmission elicited by electrical stimulation of the Schaffer collaterals in the stratum radiatum. Tetanic stimulation of Schaffer collaterals induced NMDA receptor-independent, long-term potentiation due to Ca2+ influx through the newly expressed AMPA receptors in the area densely stained with GFP. Thus, the combined use of Sindbis viral vectors with the GFP reporter allowed physiological examination of the roles of a specific gene product in synaptic function in well-characterized brain neurons.

    DOI: 10.1046/j.0953-816x.2001.01523.x

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  • Regulation of nerve growth factor release by nitric oxide through cyclic GMP pathway in cortical glial cells 査読

    HB Xiong, K Yamada, H Jourdi, M Kawamura, N Takei, DK Han, T Nabeshima, H Nawa

    MOLECULAR PHARMACOLOGY   56 ( 2 )   339 - 347   1999年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS  

    In the present study, we found that S-nitroso-N-acetyl-DL-penicillamine, a spontaneous nitric oxide (NO) generator, dose-dependently inhibited basal nerve growth factor (NGF) release from mixed glial cells. To elucidate the function of endogenous NO in the regulation of NGF release, the mixed glial cells were stimulated with lipopolysaccharide (LPS) or LPS plus interferon-gamma (IFN gamma). The results showed that LPS alone induced NGF release and moderate NO production. However, costimulation with LPS plus IFN gamma greatly enhanced NO production but significantly suppressed LPS-induced NGF release. When N-G-monomethyl-L-arginine, an NOS inhibitor, was added to the culture, the suppression of NGF release by IFN gamma was significantly reduced. Quantitative reverse transcription-polymerase chain reaction demonstrated S-nitroso-N-acetyl-DL-penicillamine was also able to inhibit the LPS-induced NGF mRNA expression. To understand the different contributions of astroglia and microglia to this phenomenon, both cell types were purified. We found purified astroglia produced high amounts of NGF but low amounts of NO. However, purified microglia produced a large amount of NO but very low amounts of NGF after stimulation with LPS or LPS plus IFN gamma. Our data also indicated the second messenger cyclic GMP, but not cyclic AMP, was able to inhibit basal NGF release. In vivo experiments confirmed that NGF protein level was significantly enhanced in rats treated with L-N-omega-nitro-arginine methyl ester and in endothelial NO synthase mutant mice. Taken together, we conclude NO derived mainly from microglia down-regulates NGF release from astroglia at the transcriptional level by stimulating cyclic GMP pathway.

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  • Cell-dependent replication potentials of HIV-1 gag mutants. 査読

    Adachi A, Tamaki M, Shimano R, Inubushi R, Naito T, Yoshida K, Oshima Y, Kawamura M, Koyama AH

    Microbes and Infection   1 ( 9 )   671 - 676   1999年

  • Enhancement of human immunodeficiency virus type 1 infectivity by Nef is producer cell-dependent 査読

    K Tokunaga, A Kojima, T Kurata, K Ikuta, H Akari, AH Koyama, M Kawamura, R Inubushi, R Shimano, A Adachi

    JOURNAL OF GENERAL VIROLOGY   79   2447 - 2453   1998年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    The growth kinetics of wild-type and nef mutant viruses of human immunodeficiency virus type 1 were comparatively analysed in several human CD4(+) cell lines. Delayed replication of nef mutant virus was observed in all cell lines examined. To determine the stage in the virus replication cycle that is affected by Nef, a single-round replication assay was performed. Initially, the expression of marker genes in transfected cells was examined in order to study the role of Nef in the late phase of infection, The results obtained indicated that Nef is dispensable during the transcription to virion production stage. Next, the effect of Nef on the early phase was investigated with a single-round infection. It was demonstrated that Nef is required in the early phase of the virus replication cycle, from virion adsorption to integration. Finally, the infectivity of virus stocks prepared from four cell lines was determined, The relative infectivity of the nef mutant from the four cell lines differed, Taken together, we conclude that Nef acts via modulation of viral particles to enhance virus infectivity in a cell-dependent manner.

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  • Suppression of HIV replication by dominant negative mutants of HIV-1 (Review) 査読

    R Inubushi, R Shimano, Y Oshima, K Yoshida, M Kawamura, A Adachi

    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE   2 ( 3 )   325 - 330   1998年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PROFESSOR D A SPANDIDOS  

    Various gag mutants of human immunodeficiency virus type 1 (HIV-1) generated in vitro were evaluated for their potentials to suppress the replication of wild-type (wt) virus. A single-round of wt virus replication in the presence of various mutant proteins was quantitatively monitored by transfection and infection experiments. Out of 38 mutants examined, 15 were demonstrated to interfere with the replication of wt HIV-I at early and/or late viral replication phase. Some of these mutants were also effective against the replication of wt HIV-2. In this review, we focus on the mutants, which are able to act against a wide variety of HN, and are very useful for future gene therapy against AIDS.

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  • Early function of HIV-1 Gag proteins is cell-dependent 査読

    M Kawamura, R Shimano, R Inubushi, H Akari, A Adachi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   248 ( 3 )   899 - 903   1998年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    Various gag gene mutants of human immunodeficiency virus type I (HIV-1) were monitored for their replication potentials and defective replication sites in various CD4-positive T-cell lines. Some matrix, capsid, and nucleocapsid mutants displayed a replication defect in a cell-dependent manner, The single-round replication assays demonstrated that these mutants were defective at an early infection phase also in a cell-dependent way, These results indicated that interaction of a cell factor(s) and Gag proteins is involved in an early process of HIV-1 replication. (C) 1998 Academic Press.

    DOI: 10.1006/bbrc.1998.9065

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  • Suppression of HIV-2 replication by HIV-1 gag mutants 査読

    R Shimano, R Inubushi, T Fukumori, M Tamaki, Y Oshima, M Kawamura, A Adachi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   248 ( 2 )   418 - 421   1998年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    Gag gene mutants of human immunodeficiency virus type 1 (HIV-1) were analyzed for their potentials of inhibiting the replication of wild-type (wt) HIV-2, the second AIDS virus, in a single-round of viral replication. Of twenty-two HIV-1 gag mutants examined, seven were found to efficiently interfere with the replication of wt HIV-2. Some mutants, which can suppress the replication of wt HIV-1, did not show this inhibitory effect. These mutants were defective at the late phase of viral replication. A mutant designated NL-C1a was demonstrated to be very effective against the replication of HIV-1 and HIV-2 in monocytic cells as well as in lymphocytic cells. (C) 1998 Academic Press.

    DOI: 10.1006/bbrc.1998.8975

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  • Inhibition of HIV replication by capsid mutant C6b 査読

    R Shimano, S Iida, T Fukumori, Y Yamamoto, M Kawamura, RA Furuta, A Adachi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   242 ( 2 )   313 - 316   1998年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    A Gag capsid mutant of human immunodeficiency virus type 1 (HIV-1) designated C6b was biologically and biochemically characterized with respect to its ability to suppress the replication of wild-type (wt) HIV. The C6b efficiently interfered with the replication of wt HIV-1 in the cleavage of Gag precursor, and also in the early replication process before or during viral DNA synthesis after viral penetration. The C6b Gag appeared to be unable to form chimeric multimers with HIV-2 Gag and failed to inhibit the replication of Wt HIV-2. (C) 1998 Academic Press.

    DOI: 10.1006/bbrc.1997.7963

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  • Complete inhibition of SIVmac replication by its capsid mutants 査読

    R Shimano, R Inubushi, K Amano, T Ogasawara, H Akari, AH Koyama, M Kawamura, A Adachi

    VIRUS GENES   17 ( 1 )   43 - 48   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KLUWER ACADEMIC PUBL  

    Mutations were introduced into a genomic region encoding the C-terminal portion of Gag capsid protein of pathogenic simian immunodeficiency virus (SIVmac239). All the mutants generated were defective for virion production and were non-infectious for monkey cells. They all efficiently suppressed the replication of wild type SIVmac in monkey cells. These results were in good agreement with those obtained for human immunodeficiency virus type 1, showing the importance of SIV/monkey model system for studies on Gag.

    DOI: 10.1023/A:1008001000878

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  • Mapping the genetic determinants of human immunodeficiency virus type 2 for cell tropism and replication efficiency 査読

    M Kawamura, R Shimano, T Ogasawara, R Inubushi, K Amano, H Akari, A Adachi

    ARCHIVES OF VIROLOGY   143 ( 3 )   513 - 521   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG WIEN  

    Two distinct infectious molecular clones of human immunodeficiency type 2 (HIV-2) were analyzed for their biological properties in six cell lines. Fourteen chimeric and ten mutant viruses were constructed from these two viral genomes to localize the genetic determinants responsible for the phenotypes. Growth property of the viruses in the cell lines, together with the biochemical data, showed that a major determinant for the viral tropism resides in the env gene. In addition, in some cell lines, the accessory genes vif and nef affected the efficiency of virus replication. Thus, like HIV-1, mutations in the auxiliary and env genes of HIV-2 contributed much to the differences in virological characteristics.

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  • Producer cell-dependent requirement of the Nef protein for efficient entry of HIV-1 into cells. 査読

    Tokunaga K, Kojima A, Kurata T, Ikuta K, Inubushi R, Shimano R, Kawamura M, Akari H, Koyama AH, Adachi A

    Biochemical and Biophysical Research Communications   250 ( 3 )   565 - 568   1998年

  • Functional domain mapping of HIV-1 gag proteins 査読

    M Kawamura, R Shimano, R Inubushi, K Amano, T Ogasawara, H Akari, A Adachi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   241 ( 2 )   317 - 320   1997年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    A series of human immunodeficiency virus type 1 (HIV-1) proviral gag gene mutants carrying bacterial CAT gene were constructed and monitored for the expression of reverse transcriptase and CAT in a highly sensitive single-round replication assay system to determine the defective replication phase in lymphocytic cells. All the mutants displayed no abnormality in the process of transcription and translation at late replication stage. In contrast, some matrix, capsid, and p6 mutants were defective at final phase, that is, assembly and virion release. Most of the mutants including nucleocapsid mutants, which showed normal phenotype at late stage, were defective at early replication phase. From the functional domain map thus obtained, it is evident that HIV-1 Gag proteins are required for both early and late replication phases. (C) 1997 Academic Press.

    DOI: 10.1006/bbrc.1997.7814

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  • Cleavage of Gag precursor is required for early replication phase of HIV-1 査読

    M Kawamura, R Shimano, R Inubushi, K Amano, T Ogasawara, H Akari, A Adachi

    FEBS LETTERS   415 ( 2 )   227 - 230   1997年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A mutant of human immunodeficiency virus type 1 (HIV-1), which is deficient for Gag precursor cleavage and noninfectious, was characterized with respect to its defective step in the viral replication phase. Upon transfection, the mutant produced a normal level of progeny virions as monitored by electron microscopy and RNA hybridization. Single-round replication assay demonstrated, in contrast, that the mutant was defective at the early phase of the replication cycle. Furthermore, no viral DNA was detected in the cells infected with the mutant. Taken together, it is concluded that maturation of Gag precursor protein of HIV-1 is required for an early event(s) before or during a coupled process of uncoating/reverse transcription. (C) 1997 Federation of European Biochemical Societies.

    DOI: 10.1016/S0014-5793(97)01131-9

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  • HIV-1 capsid mutants inhibit the replication of wild-type virus at both early and late infection phases 査読

    RA Furuta, R Shimano, T Ogasawara, R Inubushi, K Amano, H Akari, M Hatanaka, M Kawamura, A Adachi

    FEBS LETTERS   415 ( 2 )   231 - 234   1997年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    In-frame mutations were introduced into various portions of the human immunodeficiency virus type 1 (HIV-1) gag gene, and potentials of the mutants to suppress the replication of wild-type HIV-1 were monitored. In contrast to results obtained with matrix and nucleocapsid mutants, almost all capsid mutants blocked HIV-1 replication completely in single-round replication assays. A capsid mutant designated C6b was demonstrated to be one of the most efficient inhibitors for HIV-1 reported to date, and to be effective at both early and late viral replication phases. T-cells, which are engineered to express the C6b Gag in response to HIV-1 infection, were perfectly resistant to HIV-1. (C) 1997 Federation of European Biochemical Societies.

    DOI: 10.1016/S0014-5793(97)01132-0

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  • Methods for HIV/SIV gene analysis 招待 査読

    Adachi A, Kawamura M, Tokunaga K, Sakai H

    Viral Genome Mothods, chapter 3   3   43 - 53   1996年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • EARLY REPLICATION BLOCK OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN MONKEY CELLS 査読

    R SHIBATA, H SAKAI, M KAWAMURA, K TOKUNAGA, A ADACHI

    JOURNAL OF GENERAL VIROLOGY   76   2723 - 2730   1995年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    The genetic and functional basis of the replication-defective nature of human immunodeficiency virus type 1 (HIV-1) in monkey cells was studied. By the generation and characterization of chimeras between HIV-1 and simian immunodeficiency virus, the sequence encompassing the 3' half of the long terminal repeat, gag and pol genes of HIV-1 was found to be responsible for the growth restriction. Early and late phases of HIV-1 replication in monkey cells were analysed in detail using several assay systems: transfection/coculture, transcomplementation between various proviral clones carrying the CAT gene and effector clones and evaluation of transcription and reverse transcription. All the data were consistent with the notion that HIV-1 replication is blocked at a very early stage(s) such as uncoating and/or reverse transcription in monkey cells.

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  • FUNCTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 VPU PROTEIN IN VARIOUS CELL-TYPES 査読

    H SAKAI, K TOKUNAGA, M KAWAMURA, A ADACHI

    JOURNAL OF GENERAL VIROLOGY   76   2717 - 2722   1995年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    We evaluated the function of human immunodeficiency virus type 1 vpu gene in various cell lines. We established a highly sensitive system consisting of chloramphenicol acetyltransferase and reverse transcriptase assays and used it to monitor the effects of mutation of the vpu gene. In some cell lines, Vpu protein was not required at the early phase of viral replication but was important for efficient virion production. In these cells, the Vpu protein functioned effectively irrespective of the presence of intact env gene products. Likewise, CD4 gene expression had no effects on Vpu function. In the other cell lines tested, Vpu protein was not important for virion release, and the vpu mutant clone generated a normal level of progeny virions upon transfection.

    DOI: 10.1099/0022-1317-76-11-2717

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  • GENERATION AND CHARACTERIZATION OF A HOST CELL-DEPENDENT GAG GENE MUTANT OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 査読

    J SAKURAGI, H SAKAI, M KAWAMURA, K TOKUNAGA, S UEDA, A ADACHI

    VIROLOGY   212 ( 1 )   251 - 254   1995年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    An in-frame gag gene mutant of human immunodeficiency virus type 1, which carries two amino acid substitutions in the center of the p24 coding region, was constructed in vitro, and its replication properties in several cell lines were examined. In CD4-negative SW480 cells transfected with the mutant clone, synthesis and processing of viral gag, pol, and env proteins occurred normally, and viral particles were produced. Virions derived from the transfection displayed a severe replication defect when inoculated into some CD4-positive cell lines (H9 and Molt4 clone 8), but in other lines (A3.01 and M8166), the mutant virus grew fairly well. The mutant was demonstrated to be defective at an early infection phase (from adsorption to integration) in Molt4, clone 8 cells but was normal in A3.01 cells. These results indicated that the Gag-p24 protein of human immunodeficiency virus type 1 plays an important role at the early infection phase in a cell-dependent manner. (C) 1995 Academic Press, Inc.

    DOI: 10.1006/viro.1995.1478

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  • REV-DEPENDENCY OF EXPRESSION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GAG AND ENV GENES 査読

    H SAKAI, RA FURUTA, K TOKUNAGA, M KAWAMURA, M HATANAKA, A ADACHI

    FEBS LETTERS   365 ( 2-3 )   141 - 145   1995年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Structural gene expression of human immunodeficiency virus type 1 (HIV-1) requires a viral regulatory protein, Rev transactivator, We investigated Rev-dependency of HIV-1 gene expression by various reporter systems. Expression of unspliced and singly-spliced viral mRNAs was demonstrated to be differentially dependent on the Rev function, This difference of Rev-dependency was found not to be determined by cis-elements in gag, pol, and env coding sequences reported so far, and was lost when the reporter constructs containing minimum elements for Rev-responsiveness such as splice signals and rev responsive element were used for experiments. These findings indicated that fundamental structure of HIV-1 mRNA was critical for the differential regulation of gene expression by Rev transactivator.

    DOI: 10.1016/0014-5793(95)00444-E

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  • Functionality of chimeric Rev proteins of HIV SIV 査読

    RA Furuta, H Sakai, M Kawamura, K Tokunaga, M Hatanaka, A Adachi

    VIRUS GENES   11 ( 1 )   11 - 14   1995年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KLUWER ACADEMIC PUBL  

    Studies on functional compatibility of various Rev proteins derived from all known human and simian immunodeficiency virus subgroups have shown that this essential gene product is not always exchangeable among the viruses. In an attempt to map the region of Rev proteins responsible for the observed nonreciprocal complementation, hybrid genomic Rev expression vectors were constructed by exchanging the first and second exons of rev genes, and were examined for their abilities to activate reporter clones by transfection. With one exception, the second coding exon of rev gene determined the functional specificity of Rev proteins.

    DOI: 10.1007/BF01701656

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  • Functional analysis of human spuma retrovirus genome 査読

    A Adachi, H Sakai, K Tokunaga, M Kawamura

    VIRUS GENES   11 ( 1 )   15 - 20   1995年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KLUWER ACADEMIC PUBL  

    Human spuma retrovirus (HSRV) belongs to retroviruses that possess a complex genome organization. HSRV carries at least three extra genes in the region between env and the 3' long terminal repeat, which are not found in simple retroviruses. Via alternative splicing, these HSRV genes can encode several proteins. To genetically study the requirements of these viral proteins for viral replication in tissue cultures, a number of mutant viruses were constructed from an infectious molecular clone of HSRV. All mutants grew normally in the cell lines tested, except for those lacking transcriptional transactivator activity. By reporter-based transient assay systems, no Rev/Rex equivalent was detected in the HSRV proteins.

    DOI: 10.1007/BF01701657

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  • GROWTH ABILITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 AUXILIARY GENE MUTANTS IN PRIMARY BLOOD MACROPHAGE CULTURES 査読

    M KAWAMURA, T ISHIZAKI, A ISHIMOTO, T SHIODA, T KITAMURA, A ADACHI

    JOURNAL OF GENERAL VIROLOGY   75   2427 - 2431   1994年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    A strain of human immunodeficiency virus type 1 that is strictly tropic for primary human blood cell cultures was constructed in vitro. Mutational studies on the vif, vpr, vpu and nef genes of this virus were performed to evaluate their biological functions in natural target cells. For this purpose, replication properties of mutant viruses in peripheral blood mononuclear cells (PBMCs) and macrophages (PBMPs) were determined. Three phenotypes with respect to virus replication were noticed: normal or mildly retarded growth (nef and vpr mutants), impaired growth (vpu mutant), and no growth (vif mutant). These results suggest that the Vif and Vpu proteins are more important than the Nef and Vpr proteins for virus replication in PBMCs and PBMPs.

    DOI: 10.1099/0022-1317-75-9-2427

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  • HUMAN-IMMUNODEFICIENCY-VIRUS VPX IS REQUIRED FOR THE EARLY PHASE OF REPLICATION IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS 査読

    M KAWAMURA, H SAKAI, A ADACHI

    MICROBIOLOGY AND IMMUNOLOGY   38 ( 11 )   871 - 878   1994年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    Functional importance of Vpx protein of human immunodeficiency virus type 2 was evaluated in various types of cells. In 8 lymphocytic or monocytic cell lines tested, vpx mutant virus grew as well as wild-type virus. Only in primary peripheral blood mononuclear cell cultures, severely retarded growth of mutant virus was observed. No replication of vpx-minus virus was detected in primary macrophage cells. A highly sensitive single-round replication assay system was used to determine the defective replication phase in primary mononuclear cells of vpx mutant virus. In all cell lines examined, vpx mutant displayed no abnormality. In contrast, the vpx mutant was demonstrated to be defective at an early stage of the infection cycle in primary cell cultures. No evidence of a replication-defect at a late phase in primary cells of the vpx mutant was obtained by a transfection-coculture method, These results indicate that the virion-associated Vpx protein is essential for early viral replication process in natural target cells such as primary macrophages.

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  • HUMAN-IMMUNODEFICIENCY-VIRUS VPX IS REQUIRED FOR THE EARLY PHASE OF REPLICATION IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS 査読

    M KAWAMURA, H SAKAI, A ADACHI

    MICROBIOLOGY AND IMMUNOLOGY   38 ( 11 )   871 - 878   1994年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    Functional importance of Vpx protein of human immunodeficiency virus type 2 was evaluated in various types of cells. In 8 lymphocytic or monocytic cell lines tested, vpx mutant virus grew as well as wild-type virus. Only in primary peripheral blood mononuclear cell cultures, severely retarded growth of mutant virus was observed. No replication of vpx-minus virus was detected in primary macrophage cells. A highly sensitive single-round replication assay system was used to determine the defective replication phase in primary mononuclear cells of vpx mutant virus. In all cell lines examined, vpx mutant displayed no abnormality. In contrast, the vpx mutant was demonstrated to be defective at an early stage of the infection cycle in primary cell cultures. No evidence of a replication-defect at a late phase in primary cells of the vpx mutant was obtained by a transfection-coculture method, These results indicate that the virion-associated Vpx protein is essential for early viral replication process in natural target cells such as primary macrophages.

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  • CELL-DEPENDENT REQUIREMENT OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 VIF PROTEIN FOR MATURATION OF VIRUS-PARTICLES 査読

    H SAKAI, R SHIBATA, J SAKURAGI, S SAKURAGI, M KAWAMURA, A ADACHI

    JOURNAL OF VIROLOGY   67 ( 3 )   1663 - 1666   1993年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    A highly sensitive single-round infection assay using a bacterial chloramphenicol acetyltransferase was developed to analyze an early stage of human immunodeficiency virus type 1 replication. By a combination of transfection and single-round infection assay, a virus with a vif mutation, depending on host cells from which the virus was derived, was demonstrated to be defective at the early phase of infection cycle. Analysis of viral proteins synthesized in cells indicated that incorporation of the Env surface protein into virions of the vif mutant, again in a cell-dependent way, was greatly restricted. Taken together, it is concluded that the Vif protein acts through modulation of the Env protein in the virions, directly or indirectly, to enhance viral infectivity in a certain cell type.

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  • INTEGRATION IS ESSENTIAL FOR EFFICIENT GENE-EXPRESSION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 査読

    H SAKAI, M KAWAMURA, JI SAKURAGI, S SAKURAGI, R SHIBATA, A ISHIMOTO, N ONO, S UEDA, A ADACHI

    JOURNAL OF VIROLOGY   67 ( 3 )   1169 - 1174   1993年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    A mutant of human immunodeficiency virus type 1 which carries a frameshift insertion in the integrase/endonuclease region of pol gene was constructed in vitro. Upon transfection into cells, although this mutant exhibited a normal phenotype with respect to expression of gag, pol, and env genes and to generation of progeny virions, no replication-competent virus in CD4-positive cells emerged. An assay for the single-step replication of a defective viral genome dependent on trans complementation by rev protein was established and used to monitor the early phase of viral infection process. Viral clones with a mutation in the vif, vpr, or vpu gene displayed no abnormality in the early phase. In contrast, the integrase mutant did not direct a marker gene expression after infection. Together with an observation that the mutant lacked the ability to integrate, these results indicated that the integration was required for efficient viral gene expression and productive infection of human immunodeficiency virus type 1.

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  • COMPATIBILITY OF TAT AND REV TRANSACTIVATORS IN THE PRIMATE LENTIVIRUSES 査読

    H SAKAI, JI SAKURAGI, S SAKURAGI, M KAWAMURA, A ADACHI

    ARCHIVES OF VIROLOGY   129 ( 1-4 )   1 - 10   1993年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG WIEN  

    Primate immunodeficiency viruses carry a unique set of transacting regulator genes, which are essential for viral replication. The exchangeability of these Tat and Rev transactivators derived from viruses of the four major subgroups identified to date was assessed in transient transfection and infection assay systems. The human immunodeficiency virus type 1 (HIV-1), a major causative virus of human AIDS, efficiently activated the other viruses. In contrast, the tat and rev gene products of HIV-2, SIV(AGM) (Virus of the African green monkey), and SlV(MND) (virus of the mandrill) did not fully transactivate the HIV-1. In particular, the rev of HIV-1 was not substantially replaced by those of the other viruses. The result that HIV-1 is distinct from the other immunodeficiency viruses with respect to the compatibility of two transactivators gives a firm functional basis for the unique phylogenetic position of HIV-1.

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  • ISOLATION AND CHARACTERIZATION OF SIMIAN IMMUNODEFICIENCY VIRUS FROM AFRICAN WHITE-CROWNED MANGABEY MONKEYS (CERCOCEBUS-TORQUATUS-LUNULATUS) 査読

    K TOMONAGA, J KATAHIRA, M FUKASAWA, MA HASSAN, M KAWAMURA, H AKARI, T MIURA, T GOTO, M NAKAI, M SULEMAN, M ISAHAKIA, M HAYAMI

    ARCHIVES OF VIROLOGY   129 ( 1-4 )   77 - 92   1993年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG WIEN  

    Forty-eight of 236 sera from seven species of African non-human primates in Kenya, including those of white-crowned mangabey monkeys (Cercocebus torquatus lunulatus) had antibodies to simian immunodeficiency viruses (SIVs). Isolates of simian lentivirus were obtained from seropositive white-crowned mangabey monkeys which are indigenous in West Africa. This virus, designated as SIV(WCM), appeared morphologically similar to HIV by electron microscopy, showed Mg2+-dependent reverse transcriptase activity, and induced cytopathic effects in human CD 4-positive cells. Western blotting analysis revealed that env products of SIV(WCM) cross-reacted with those of SIV(AGM) more strongly than with those of HIV-I and SIV(MAC), and clear hybridization bands were detected with an SIV(AGM) probe. For comparison of the virus sequence with those of other primate lentiviruses, part of the pol gene and the long terminal repeats (LTRs) were amplified and cloned. Sequencing showed that SIV(WCM) isolates were closely related to SIV(AGM) isolates. This study suggested that SIV(AGM) from the Cercopithecus genus and SIV(WCM) from the Cercocebus genus may be members of an SIV group that is genetically distinct from the SIV from a sooty mangabey monkey (SIV(SMM)) of the genus Cercocebus, to which the white-crowned mangabey monkey also belongs.

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  • INFECTION OF MACAQUE MONKEYS WITH A CHIMERIC HUMAN AND SIMIAN IMMUNODEFICIENCY VIRUS 査読

    S SAKURAGI, R SHIBATA, R MUKAI, T KOMATSU, M FUKASAWA, H SAKAI, JI SAKURAGI, M KAWAMURA, K IBUKI, M HAYAMI, A ADACHI

    JOURNAL OF GENERAL VIROLOGY   73   2983 - 2987   1992年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Two macaque monkeys were inoculated with a chimeric human and simian immunodeficiency virus carrying the tat, rev, vpu and env genes of human immunodeficiency virus type 1. Infectious virus was recovered from one of the monkeys at 2 and 6 weeks post-infection. The hybrid nature of the isolated viruses was verified by Southern and Western blotting analyses. Both of the monkeys infected with the chimera elicited a humoral antibody response against the virus.

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  • SEQUENCES RESPONSIBLE FOR EFFICIENT REPLICATION OF SIMIAN IMMUNODEFICIENCY VIRUS SIV(MND) IN CELLS OF THE MONOCYTE MACROPHAGE LINEAGE 査読

    H SAKAI, S SAKURAGI, JI SAKURAGI, M KAWAMURA, R SHIBATA, A ADACHI

    JOURNAL OF GENERAL VIROLOGY   73   2989 - 2993   1992年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    We determined the susceptibility of monocytic cell lines to infection with viral strains derived from two infectious clones of simian immunodeficiency virus isolated from a mandrill. One of the strains, which replicates poorly in T cell lines, was found to grow more rapidly than the other in these cells. The viral determinant for this property was genetically mapped within the env, gene encoding a surface protein. Six amino acid substitutions identified appeared to be located outside of the domains corresponding to human immunodeficiency virus type 1 env functional domains such as the CD4-binding and V3 loop regions.

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  • ISOLATION AND CHARACTERIZATION OF A HIGHLY DIVERGENT HIV-2[GH-2] - GENERATION OF AN INFECTIOUS MOLECULAR CLONE AND FUNCTIONAL-ANALYSIS OF ITS REV-RESPONSIVE ELEMENT IN RESPONSE TO PRIMATE RETROVIRUS TRANSACTIVATORS (REV AND REX) 査読

    M KAWAMURA, J KATAHIRA, M FUKASAWA, JI SAKURAGI, KI ISHIKAWA, M NAKAI, JAA MINGLE, M OSEIKWASI, VBA NETTY, H AKARI, O HISHIDA, K TOMONAGA, T MIURA, M HAYAMI

    VIROLOGY   188 ( 2 )   850 - 853   1992年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1016/0042-6822(92)90540-6

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  • BIOLOGICAL CHARACTERIZATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 AND TYPE-2 MUTANTS IN HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS 査読

    H AKARI, J SAKURAGI, Y TAKEBE, K TOMONAGA, M KAWAMURA, M FUKASAWA, T MIURA, T SHINJO, M HAYAMI

    ARCHIVES OF VIROLOGY   123 ( 1-2 )   157 - 167   1992年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG WIEN  

    Mutants of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), which have been shown to be infectious in established cell lines, were tested for ability to replicate and induce syncytium formation in human peripheral blood mononuclear cells (PBMC). The vpu mutant of HIV-1 showed depressed kinetics of replication in an established T cell line, as reported previously, but in PBMC, its replication was similar to that of the wild type virus. The vpx gene of HIV-2 was required for efficient virus propagation in PBMC, but not in an established T cell line, as previously reported. However, the growth rates of the vpx mutant in PBMC preparations from two individuals were different. The results of experiments on infection of PBMC with the vif and vpr mutants of HIV-1 and HIV-2 were essentially consistent with previous results of infection of established T cell lines. No negative effect of the nef gene products of HIV-1 and HIV-2 was observed. The abilities of the wild type virus and the mutants of HIV-1 to induce syncytium formation in both PBMC and established cell lines were similar. In contrast, neither the wild type nor any of the mutants of HIV-2 induced syncytium formation in PBMC. These results suggest that the functions of some genes can be detected only in mixed populations or primary cells such as PBMC. Studies on the roles of these genes in PBMC may provide a better understanding of their functions in vivo.

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  • PRODUCTION AND CHARACTERIZATION OF MOUSE MONOCLONAL-ANTIBODIES AGAINST THE TRANSMEMBRANE PROTEIN OF A HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2 査読

    H KOMATSU, A YAMASHITA, H TOZAWA, Y MIZUTANI, M HONDA, M KAWAMURA, M HAYAMI

    AIDS RESEARCH AND HUMAN RETROVIRUSES   7 ( 12 )   999 - 1005   1991年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MARY ANN LIEBERT INC PUBL  

    We established seven hybridoma clones producing monoclonal antibodies (MAbs) against the envelope transmembrane protein (TMP) of a Ghanian isolate of human immunodeficiency virus type 2 (HIV-2[GH-1]) from mice immunized with the detergent-disrupted purified whole virus. The MAbs were found to react with 35 kilodalton (kD) TMP of the HIV-2[GH-1] virus in a Western blot assay (WB). Two of these MAbs recognized 135 kD proteins in addition to TMP in the lysate of HIV-2[GH-1]-infected cells. Two other MAbs cross-reacted with viral components corresponding to TMPs of HIV-2ROD and SIV(MAC) isolates in a Western blot. Results of competitive binding assay suggest that there are at least three epitopes on a TMP molecule of the HIV-2[GH-1] isolate. The MAbs did not inhibit syncytium formation between HIV-2[GH-1]-infected MOLT-4 cells and MOLT-4 clone 8 cells, nor virus infection to MOLT-4 clone 8 cells.

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  • FUNCTIONAL-ANALYSIS OF LONG TERMINAL REPEATS DERIVED FROM 4 STRAINS OF SIMIAN IMMUNODEFICIENCY VIRUS SIVAGM IN RELATION TO OTHER PRIMATE LENTIVIRUSES 査読

    J SAKURAGI, M FUKASAWA, R SHIBATA, H SAKAI, M KAWAMURA, H AKARI, T KIYOMASU, A ISHIMOTO, M HAYAMI, A ADACHI

    VIROLOGY   185 ( 1 )   455 - 459   1991年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1016/0042-6822(91)90798-G

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  • COMPATIBILITY OF REV GENE ACTIVITY IN THE 4 GROUPS OF PRIMATE LENTIVIRUSES 査読

    H SAKAI, R SHIBATA, J SAKURAGI, T KIYOMASU, M KAWAMURA, M HAYAMI, A ISHIMOTO, A ADACHI

    VIROLOGY   184 ( 2 )   513 - 520   1991年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1016/0042-6822(91)90421-7

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  • GENERATION OF A CHIMERIC HUMAN AND SIMIAN IMMUNODEFICIENCY VIRUS INFECTIOUS TO MONKEY PERIPHERAL-BLOOD MONONUCLEAR-CELLS 査読

    R SHIBATA, M KAWAMURA, H SAKAI, M HAYAMI, A ISHIMOTO, A ADACHI

    JOURNAL OF VIROLOGY   65 ( 7 )   3514 - 3520   1991年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    We constructed five chimeric clones between human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV(MAC)) and four SIV(MAC) mutants by recombinant DNA techniques. Three chimeric clones and all mutants with an alteration in either the vif, vpx, vpr, or nef gene were infectious to human CD4-positive cell lines. The susceptibility of macaque monkey peripheral blood mononuclear cells (PBMC) to infection by these mutants and chimeras was examined in vitro. Macaque PBMC supported the replication of wild-type and vpx, vpr, and nef mutant SIV(MAC) strains. A chimera carrying the long terminal repeats (LTRs), gag, pol, vif, and vpx of SIV(MAC) and tat, rev, vpu, and env of HIV-1 was also replication competent in PBMC. In contrast, HIV-1, the vif mutant of SIV(MAC), a chimera containing rev and env of SIV(MAC), and a chimera containing vpx, vpr, tat, rev, and env of SIV(MAC) did not grow in PBMC. Western immunoblotting analysis of the replicating chimera in PBMC confirmed the hybrid nature of the virus. These data strongly suggested that the sequence important for macaque cell tropism lies within the LTR, gag, pol, and/or vif sequences of the SIV(MAC) genome.

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  • GEOGRAPHICAL-DISTRIBUTION OF SUBJECTS SEROPOSITIVE FOR HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1 IN PAPUA-NEW-GUINEA 査読

    J IMAI, S TERASHI, T TALONU, H KOMODA, T TAUFA, GT NURSE, D BABONA, K YAMAGUCHI, H NAKASHIMA, K ISHIKAWA, M KAWAMURA, M HAYAMI

    JAPANESE JOURNAL OF CANCER RESEARCH   81 ( 12 )   1218 - 1221   1990年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE CANCER ASSOCIATION  

    Of 1471 sera collected from 1986 to 1989 in Papua New Guinea (PNG), 2.2% were found to be positive for anti-HTLV-1 antibody by successive particle agglutination and immunofluorescence tests. The seropositive rate varied in different provinces and was higher in the coastal areas of the main island and in neighboring small islands than in the highlands of PNG. The frequency of HTLV-1 infection of children was higher, but the age-dependent increase in antibody positivity, generally observed in other HTLV-1 endemic areas of the world, was not clear in PNG. No difference was observed in antibody prevalence in males and females in this study.

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  • ESTABLISHMENT OF A PHYLOGENETIC SURVEY SYSTEM FOR AIDS-RELATED LENTIVIRUSES AND DEMONSTRATION OF A NEW HIV-2 SUBGROUP 査読

    T MIURA, J SAKURAGI, M KAWAMURA, M FUKASAWA, EN MORIYAMA, T GOJOBORI, K ISHIKAWA, JAA MINGLE, VBA NETTEY, H AKARI, M ENAMI, H TSUJIMOTO, M HAYAMI

    AIDS   4 ( 12 )   1257 - 1261   1990年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:RAPID SCIENCE PUBLISHERS  

    We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridizes with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2ALT(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIV(mac)/SIV(sm) in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.

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  • MULTIPLE ANTIGENIC EPITOPES EXPRESSED ON GAG PROTEINS, P26 AND P15, OF A HUMAN-IMMUNODEFICIENCY-VIRUS (HIV) TYPE-2 AS DEFINED WITH A LIBRARY OF MONOCLONAL-ANTIBODIES 査読

    H KOMATSU, H TOZAWA, M KAWAMURA, T KODAMA, M HAYAMI

    AIDS RESEARCH AND HUMAN RETROVIRUSES   6 ( 7 )   871 - 881   1990年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MARY ANN LIEBERT INC PUBL  

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  • IMMUNOLOGICAL REACTIVITIES OF GHANAIAN SERA WITH HIV-1, HIV-2, AND SIMIAN IMMUNODEFICIENCY VIRUS SIVAGM 査読

    M KAWAMURA, K ISHIKAWA, JAA MINGLE, M OSEIKWASI, SN AFOAKWA, VBA NETTEY, TR CHOSA, M HAYAMI

    AIDS   3 ( 9 )   609 - 611   1989年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:RAPID SCIENCE PUBLISHERS  

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  • HIV-2 IN WEST-AFRICA IN 1966 査読

    MOHAMMED, I, TO HARRY, A NASIDI

    LANCET   1 ( 8647 )   1137 - 1137   1989年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LANCET LTD  

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  • ISOLATION AND CHARACTERIZATION OF HIV-2 FROM AN AIDS PATIENT IN GHANA 査読

    K ISHIKAWA, H TSUJIMOTO, M NAKAI, JAA MINGLE, M OSEIKWASI, SE AGGREY, VBA NETTEY, SN AFOAKWA, M FUKASAWA, T KODAMA, M KAWAMURA, M HAYAMI

    AIDS   2 ( 5 )   383 - 388   1988年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:RAPID SCIENCE PUBLISHERS  

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  • SEROLOGICAL AND PATHOLOGICAL-STUDIES OF NEWCASTLE-DISEASE VIRUSES ISOLATED FROM CAGED BIRDS FROM SOUTHEAST-ASIA 査読

    M KAWAMURA, K NEROME, H KODAMA, H IZAWA, T MIKAMI

    AVIAN DISEASES   31 ( 3 )   564 - 569   1987年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC AVIAN PATHOLOGISTS  

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  • ISOLATION OF ORTHOMYXOVIRUS AND PARAMYXOVIRUS FROM MIGRATING FERAL DUCKS IN JAPAN 査読

    T MIKAMI, M KAWAMURA, T KONDO, T MURAI, M HORIUCHI, H KODAMA, H IZAWA, H KIDA

    VETERINARY RECORD   120 ( 17 )   417 - 418   1987年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BRITISH VETERINARY ASSOC  

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  • ANTIGENIC VARIATION OF NEWCASTLE-DISEASE VIRUSES ISOLATED FROM WILD DUCKS IN JAPAN 査読

    M KAWAMURA, K NAGATAMATSUBARA, K NEROME, N YAMANE, H KIDA, H KODAMA, H IZAWA, T MIKAMI

    MICROBIOLOGY AND IMMUNOLOGY   31 ( 8 )   831 - 835   1987年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    DOI: 10.1111/j.1348-0421.1987.tb03144.x

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  • PATHOGENICITY AND IMMUNOGENICITY IN CHICKENS OF NEWCASTLE-DISEASE VIRUSES ISOLATED FROM WILD DUCKS 査読

    M KAWAMURA, T MIKAMI, H KODAMA, H IZAWA

    ARCHIVES OF VIROLOGY   95 ( 1-2 )   149 - 156   1987年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG WIEN  

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  • PATHOGENICITY AND IMMUNOGENICITY IN CHICKENS OF NEWCASTLE-DISEASE VIRUSES ISOLATED FROM WILD DUCKS 査読

    M KAWAMURA, T MIKAMI, H KODAMA, H IZAWA

    ARCHIVES OF VIROLOGY   95 ( 1-2 )   149 - 156   1987年

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    記述言語:英語   掲載種別:学位論文(修士)   出版者・発行元:SPRINGER-VERLAG WIEN  

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MISC

  • Characterization of novel bicistronic Sindbis virus vectors, SinEGdsp and SinIRES-EG, in cultured neurons. (Minireview)

    Kawamura M, Namba H, Otsu Y, Hayashi Y, Takei N. Nawa H

    Recent Research Development in Neurochemistry   2003年

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • HIV複製の分子基盤.

    足立昭夫, 川村名子

    医学のあゆみ   1996年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • ヒト免疫不全ウイルス (HIV) のアクセサリー遺伝子

    川村名子, 徳永研三, 足立昭夫

    細胞工学   1995年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • ヒト免疫不全ウイルス (HIV) の制御遺伝子の機能

    徳永研三, 古田里佳, 川村名子, 足立昭夫

    蛋白質核酸酵素   1995年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • HIV遺伝子の構造と機能

    川村名子, 酒井博幸, 足立昭夫

    日本臨床増刊号   1993年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • HIVの制御遺伝子と遺伝子治療. エイズー基礎から臨床へ

    川村名子, 酒井博幸, 足立昭夫

    臨床医のための実験医学シリーズ12   1993年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • AIDSウイルスーその増殖機構をめぐって

    川村名子, 酒井博幸, 足立昭夫

    感染症   1993年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • HIVの起源

    足立昭夫, 酒井幸博, 川村名子

    Mebio   1993年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • レトロウイルス

    酒井博幸, 川村名子, 足立昭夫

    化学工業   1993年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • ヒト免疫不全ウイルス(HIV) の生物学.

    酒井博幸, 川村名子, 桜木小百合, 岩谷靖雅, 足立昭夫

    治療   1993年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • HIV-1の増殖機構

    酒井博幸, 川村名子, 桜木小百合, 桜木淳一, 足立昭夫

    Minophagen Medical Review   1993年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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講演・口頭発表等

  • Patients with anti-MOG antibody-positive optic neuritis demonstrated characteristic clinical features and certain binding epitope on human MOG extracellular domain

    Tanaka K, Kawamura M, Koike N, Oone M, Sakimura K, Kezuka T, Ishikawa H

    ECTRIMS  2018年10月 

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  • Progress of genetically modified mice for A3 group 招待 国際会議

    Kawamura Meiko

    Coference on Autophagy at Niigata University  2018年3月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Niigata University  

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  • Exploring the epitopes of anti-MOG antibodies in the patients with inflammatory demyelinating disease presenting various clinical phenotypes 国際会議

    Naoto Koike, Meiko Kawamura, Moe Oono, Kenji Sakimura, Keiko Tanaka

    World Congress of Neurology  2017年 

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    記述言語:英語   会議種別:ポスター発表  

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  • デルタ型グルタミン酸受容体サブユニット機能とその量的関連

    中本 千尋, 川村 名子, 夏目 里恵, 阿部 学, 渡辺 雅彦, 崎村 建司

    日本生物学的神経医学会  2016年 

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    記述言語:英語   会議種別:ポスター発表  

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  • インターロイキン1によるグルタミン酸トランスポーターの発現調節ー脳内炎症におけるグリア細胞の役割を考察する 招待

    川村 名子

    京都大学ウイルス研究所 学術セミナー  2005年12月  京都大学ウイルス研究所

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • インターロイキン1によるグリア細胞の機能的変換

    川村 名子, 武井 延之, 那波 ひろゆき

    第70回 日本インターフェロン・サイトカイン学会  2005年6月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • 転写因子NF-kBを介したインターロイキン1によるグリア細胞の機能変化

    川村 名子, 武井 延之, 那波 宏之

    神経化学会  2004年8月 

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    記述言語:英語   会議種別:ポスター発表  

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  • 脳に高発現する膜結合型、新規ユビキチンライゲース(Momo及びSakura)の生化学的解析

    川村 名子, 荒木 一明, 鈴木 俊顕, 松田 憲之, 神辺 太樹, 千葉 智樹, 田中 啓二, 那波 宏之

    神経化学会  2003年8月 

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    記述言語:英語   会議種別:ポスター発表  

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  • 神経細胞とグリア細胞で同定されたZnフィンガー/RINGフィンガー含有分子、MomoとSakura

    荒木 一明, 川村 名子, 神辺 太樹, 石井 京子, 市川 富夫, 熊西 敏郎, 田中 啓二, 武井 延之, 那波 宏之

    神経化学会  2003年8月 

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    記述言語:英語   会議種別:ポスター発表  

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  • The surface expression of AMPA receptor is regulated by BDNF through the modulation of NSF-GluR2 interaction. 国際会議

    Narisawa-Saito M, Iwakura Y, Kawamura M, Araki K, Hussam J, Takei N, Nawa H

    31st Annual Meeting, Society for Neuroscience, San Diego, CA. USA  2001年11月 

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    記述言語:英語   会議種別:ポスター発表  

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  • AMPA型グルタミン酸受容体のC末端を介した受容体内在化機構

    岩倉 百合子, 堀川洋, 茨木 京子, 永野 忠聖, 川村 名子, 武井 延之, 那波 宏之

    神経化学会  2001年9月 

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    記述言語:英語   会議種別:ポスター発表  

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  • Sindbis virus-mediated expression of Ca2+-permeable AMPA receptors in CA1 hippocampal neurons and induction of NMDA receptor-independent long-term poteintiation. 国際会議

    Kakegawa W, Okada T, Iino M, Yamada N, Tsuzuki K, Kawamura M, Nawa H, Ozawa S

    30th Annual Meeting, Society for Neuroscience, New Orleans, LA. USA  2000年11月 

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    記述言語:英語   会議種別:ポスター発表  

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  • Sindbis virus vector carrying GFP as a reporter gene: electrophysilogical and cell biological evaluation in the neocortical neuron. 国際会議

    Namba H, Kawamura M, Okada M, Otsu Y, Nawa H

    29th Annual Meeting, Society for Neuroscience, Miami Beach, FL. USA  1999年10月 

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    記述言語:英語   会議種別:ポスター発表  

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  • RNAウイルスベクターを用いた中枢神経細胞への遺伝子導入

    難波 寿明, 川村 名子, 岡田 誠剛, 大津 洋, 那波 宏之

    日本神経科学大会  1999年7月 

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    記述言語:英語   会議種別:ポスター発表  

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  • Functional analysis of HIV-1 gag proteins 国際会議

    Kawamura, M, Kitamura, T, Ishimoto, A, Adachi, A

    10th International Conference on AIDS Yokohama, Japan.  1994年8月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • Production of chimeric virus-like particles containing the HIV-1 gag/pol and HIV-2 gag gene products. 国際会議

    Kawamura, M, Shioda, T, Kitamura, T, Iwakura, Y, Shibuta, H

    8th International Conference on AIDS Amsterdam, Netherlands  1992年7月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • グレイマンガベイからのサル免疫不全ウイルス(SIV)の分離とその性状解析

    朝長 啓造, 片平 じゅん, Hassan MA, 川村 名子, 三浦 智行, 速水 正憲

    日本獣医学会学術集会  1991年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • Isolation of a highly divergent HIV-2 distinct from known isolates of HIV-2 and SIV. 国際会議

    Kawamura, M, Sakuragi, J, Fukasawa, M, Miura, T, Gojobori, T, Moriyama, E.N, Mingle, J.A.A, Netty, V.B.A, Hayami, M

    6th International Conference on AIDS San Francisco, USA  1990年6月 

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    記述言語:英語   会議種別:口頭発表(招待・特別)  

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  • Isolation and Characterization of HIV-2 from AIDS patient in Ghana 国際会議

    Ishikawa, K, Tsujimoto, H, Hasegawa, A, Miki, K, Mingle, J.A, Kawamura M, Hayami M

    4th International Conference on ADIS Stockholm, Sweden1988  1988年6月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • カモ由来ニューカッスル病ウイルスのニワトリヒナに対する病原性

    川村 名子

    日本獣医学会学術集会  1985年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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▶ 全件表示

産業財産権

  • 血液を用いた統合失調症の診断方法

    那波 宏之, 染矢 俊幸, 村竹 辰之, 川村 名子

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    出願番号:特開2003-334170  出願日:2003年9月

    公表番号:特願2004-135667  公表日:2004年5月

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  • 精神分裂病により発現量が変化する遺伝子を規定する核酸を解析する方法

    那波 宏之, 高橋 均, 入谷 修司, 川村 名子

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    出願番号:特願2002-036937  出願日:2002年2月

    公表番号:特開2003-235557  公表日:2003年8月

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共同研究・競争的資金等の研究

  • 遺伝子組み換えマウスの作製

    2010年4月 - 現在

    科学研究費補助金 

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    資金種別:競争的資金

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  • 統合失調症関連遺伝子の探索と生化学的解析

    1998年6月 - 2007年3月

    科学技術振興調整費 

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    資金種別:競争的資金

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  • 霊長類由来レトロウイルスの分子遺伝学

    1987年10月 - 1995年3月

    科学研究費補助金 

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    資金種別:競争的資金

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  • 野生カモ類由来パラミキソウイルスのニワトリへの病原性と免疫原性について

    1983年4月 - 1985年3月

    科学研究費補助金 

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    資金種別:競争的資金

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担当経験のある授業科目(researchmap)

  • 化学

    機関名:新潟青陵大学

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  • 感染免疫学

    機関名:新潟青陵大学

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