2021/09/20 更新

写真a

ホリイ アラタ
堀井 新
HORII Arata
所属
教育研究院 医歯学系 医学系列 教授
医歯学総合研究科 生体機能調節医学専攻 感覚統合医学 教授
職名
教授
外部リンク

学位

  • 前庭刺激の視床下部ヒスタミン遊離に対する影響 ( 1994年3月   大阪大学 )

研究分野

  • ライフサイエンス / 耳鼻咽喉科学

経歴(researchmap)

  • 新潟大学   医歯学総合研究科 生体機能調節医学専攻 感覚統合医学   教授

    2015年4月 - 現在

      詳細を見る

経歴

  • 新潟大学   医歯学総合研究科 生体機能調節医学専攻 感覚統合医学   教授

    2015年4月 - 現在

学歴

  • 大阪大学   医学系研究科

    - 1994年3月

      詳細を見る

    国名: 日本国

    researchmap

  • 徳島大学   医学部

    - 1989年3月

      詳細を見る

    国名: 日本国

    researchmap

留学歴

  • オタゴ大学医学部薬理学教室   visiting research staff

    1998年10月 - 2000年9月

 

論文

  • Reliability and validity of the Japanese version of the Glasgow Edinburgh Throat Scale (GETS-J): Use for a symptom scale of globus sensation 査読

    Nao Takahashi, Kaori Mori, Hironori Baba, Takanobu Sasaki, Masaaki Ohno, Fumio Ikarashi, Naotaka Aizawa, Kunihiro Sato, Akio Tsuchiya, Hideyuki Hanazawa, Masahiko Tomita, Yamato Kubota, Yuka Morita, Kuniyuki Takahashi, Arata Horii

    Auris Nasus Larynx   45 ( 5 )   1041 - 1046   2018年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ireland Ltd  

    Objective: Globus sensation, a feeling of lump or something stuck in the throat, could be caused by structural, functional, and psychogenic diseases. Due to a possible multifactorial nature of the disease, neither diagnosing test battery nor standard treatment for globus sensation has been established. Therefore, a questionnaire to accurately identify globus patients and evaluate the severity of the disease is desired. Glasgow Edinburgh Throat Scale (GETS) is a 10-item questionnaire about the throat symptoms consisting of three subscales relating to dysphagia, globus sensation, and pain/swelling in the throat. It was reported that globus patients marked high scores specifically for the globus scale among three scales, indicating that GETS can be used as a valid symptom scale for globus sensation. Aims of this study were to translate GETS into Japanese and to test its reliability and validity. Methods: Fifty-five patients complaining of globus sensation without abnormal endoscopic and CT findings were enrolled into the study. They were asked to answer the questions of GETS translated into Japanese (GETS-J). Reliability (internal consistency) of the questionnaire was tested using Cronbach's coefficient alpha. To test the validity, principal components analysis was used to identify the factorial structure of the questionnaire and GETS-J data were compared with those reported in the original GETS. Contribution of psychiatric comorbidities to globus sensation was also investigated by examining the correlation between Hospital Anxiety and Depression Scale (HADS) and GETS-J. Results: Reliability of the questionnaire examined by the Cronbach's coefficient alpha was satisfactory and all higher than 0.75. Principal components analysis identified following three questions as the globus scale: Q1, Feeling something stuck in the throat
    Q5, Throat closing off
    Q9, Want to swallow all the time. Somatic distress, i.e., patients’ reaction to throat symptoms, was significantly correlated with globus scale (r = 0.680). Anxiety component of HADS was significantly correlated with somatic distress but not with globus scale. These results were consistent with those of the original GETS except for the replacement of Q3 (discomfort/irritation in the throat) to Q5 (throat closing off) for globus scale in GETS-J. Conclusion: Translation of GETS into Japanese showed high reliability and validity, suggesting that translation and cross-cultural adaptation were not problematic. High correlation of globus scale of GETS-J with somatic distress indicated that GETS-J could be a useful questionnaire to identify the globus patients and evaluate the severity of the disease. Anxiety may complicate the somatic distress in patients with globus sensation.

    DOI: 10.1016/j.anl.2018.02.001

    Scopus

    PubMed

    researchmap

  • Multicenter phase I/II study of chemoradiotherapy with high-dose CDDP for head and neck squamous cell carcinoma in Japan 査読

    Hiroshi Matsuyama, Keisuke Yamazaki, Ryuichi Okabe, Yushi Ueki, Ryusuke Shodo, Jo Omata, Yuichiro Sato, Hisayuki Ota, Takeshi Takahashi, Masahiko Tomita, Yusuke Yokoyama, Takafumi Togashi, Hidefumi Aoyama, Eisuke Abe, Yasuo Saijo, Kouji Katsura, Marie Soga, Tadashi Sugita, Yasuo Matsumoto, Emiko Tsuchida, Arata Horii

    Auris Nasus Larynx   45 ( 5 )   1086 - 1092   2018年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ireland Ltd  

    Objective: Recent data indicated that concurrent chemoradiotherapy (CCRT) using high dose cisplatin (CDDP) is the most useful treatment for advanced head and neck squamous cell carcinoma (SCC). Regarding the dose of CDDP, 100 mg/m2 is most recommended in Western countries. However, in terms of a balance of efficacy and adverse events, appropriate dose of cytotoxic drugs such as CDDP may be different among the different ethnic groups. In this multicenter phase I/II study, we aimed to identify the optimal dose of CDDP in CCRT for patients with advanced head and neck SCC in the Japanese. Methods: Patients were eligible for inclusion if they had head and neck SCC that was treated with radical CCRT comprising whole-neck irradiation of the primary lesion and level II–IV lymph nodes on both sides. For the phase I study, a CDDP dose was 70 mg/m2 for level 0, 80 mg/m2 for level 1, and 100 mg/m2 for level 2. Maximum tolerated dose (MTD) and dose-limiting toxicity (DLT) were examined by phase I trial, by which CDDP dose for phase II was determined. The primary endpoint for the phase II was CCRT completion rate, and the secondary endpoint was full-dose-CCRT completion rate, the percentage of patients receiving a total CDDP dose of ≥200 mg/m2, response rate, and incidences of adverse events. Results: A CDDP dose of 100 mg/m2 was the MTD for phase I, and the recommended dose for phase II was 80 mg/m2. Forty-seven patients were evaluated in the phase II trial. CCRT completion rate, full-dose-CCRT rate, and the percentage of patients receiving a total CDDP dose of ≥200 mg/m2, were 93.6%, 78.7%, and 93.6%, respectively. One patient (2.1%) developed grade 2 renal dysfunction, and no patient developed febrile neutropenia or a grade 4 adverse event. Conclusion: The present phase I study indicated that a CDDP dose of 80 mg/m2 is the optimal dose in terms of safety. The phase II study revealed that CCRT completion rate, response rate, and rates of adverse events were not inferior for a CDDP dose of 80 mg/m2 as compared with a dose of 100 mg/m2, and a dose of 80 mg/m2 is therefore recommended in CCRT for the Japanese. This study was registered with the University Hospital Medical Information Network Clinical Trials Registry (UMIN-CTR
    identification No. UMIN000010369).

    DOI: 10.1016/j.anl.2018.02.008

    Scopus

    PubMed

    researchmap

  • Surgical treatment of enterovirus D68 brainstem encephalitis-induced dysphagia 査読

    Takafumi Togashi, Hironori Baba, Meiko Kitazawa, Nao Takahashi, Yasuhiro Samejima, Eiji Yumoto, Arata Horii

    Auris Nasus Larynx   45 ( 5 )   1093 - 1097   2018年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ireland Ltd  

    Cluster of acute flaccid paralysis and cranial nerve dysfunction was associated with a 2014 outbreak of enterovirus D68 (EV-D68) respiratory illness in US. We describe a 33 year-old male patient of refractory dysphagia due to EV-D68-induced brainstem encephalitis successfully treated by surgery. Following acute upper respiratory tract infection, he developed dysphagia and bilateral facial paralysis. A coughing reflex was readily produced when the laryngopharyngeal fiberscope touched the epiglottis, however, water infusion induced only very weak and slow swallowing reflex, suggesting that only motor component was impaired but sensory function was preserved during swallowing. Despite eight months-conservative rehabilitations, Food Intake Level Scale (FILS) remained level 4. Therefore, corrective surgeries including cricopharyngeal myotomy, laryngeal suspension, and pharyngeal flap were performed. Thirty-six days after surgery, FILS rapidly and dramatically improved to level 8. This is the first report describing a successful surgical intervention for EV-D68-induced refractory dysphagia. Surgical treatment was suitable for EV-D68-induced dysphagia, perhaps because sensory function was preserved and only motor disturbance was present during the pharyngeal stage of swallowing.

    DOI: 10.1016/j.anl.2017.12.004

    Scopus

    PubMed

    researchmap

  • A high jugular bulb and poor development of perivestibular aqueductal air cells are not the cause of endolymphatic hydrops in patients with Ménière's disease. 査読 国際誌

    Oya R, Imai T, Sato T, Uno A, Watanabe Y, Okazaki S, Ohta Y, Kitahara T, Horii A, Inohara H

    Auris, nasus, larynx   45 ( 4 )   693 - 701   2018年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.anl.2017.09.014

    PubMed

    researchmap

  • Molecular diversity of clustered protocadherin-α required for sensory integration and short-term memory in mice. 査読

    Yamagishi T, Yoshitake K, Kamatani D, Watanabe K, Tsukano H, Hishida R, Takahashi K, Takahashi S, Horii A, Yagi T, Shibuki K

    Scientific reports   8 ( 1 )   9616   2018年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-018-28034-4

    PubMed

    researchmap

  • Histological detection of dynamic glial responses in the dysmyelinating Tabby-jimpy mutant brain 査読

    Masanao Ikeda, M. Ibrahim Hossain, Li Zhou, Masao Horie, Kazuhiro Ikenaka, Arata Horii, Hirohide Takebayashi

    Anatomical Science International   93 ( 1 )   119 - 127   2018年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Tokyo  

    Oligodendrocytes (OLs) are glial cells that form myelin sheaths surrounding the axons in the central nervous system (CNS). Jimpy (jp) mutant mice are dysmyelinating disease models that show developmental abnormalities in myelinated OLs in the CNS. The causative gene in jp mice is the proteolipid protein (PLP) located on the X chromosome. Mutations in the jp allele result in exon 5 skipping and expression of abnormal PLP containing a C-terminal frame shift. Many lines of evidence suggest that abnormal PLP in OLs results in endoplasmic reticulum (ER) stress and cell death. To histologically detect glial responses in the jp mutant brain, we performed staining with lineage-specific markers. Using OL markers and OL progenitor cell marker staining, we identified reduced numbers of OL lineage cells in the jp mutant brain. Nuclear staining of the transcription factor Olig1 was observed in the Tabby-jp brain, whereas cytoplasmic Olig1 staining was observed in the wild-type brain at postnatal day 21, suggesting that active myelination was present in the mutant brain. Many microglial cells with activated morphology and intensive staining of CD11b microglia marker were observed in the internal capsule of the mutant brain, a region of white matter containing residual OLs. Activated astrocytes with high glial fibrillary acidic protein-immunoreactivity were also mainly observed in white matter. Finally, we performed in situ hybridization using C/EBP homologous protein (CHOP) antisense probes to detect ER stressed cells. CHOP mRNA was strongly expressed in residual OLs in the Tabby-jp mutant mice at postnatal stages. These data show that microglia and astrocytes exhibit dynamic glial activation in response to cell death of OLs during Tabby-jp pathogenesis, and that CHOP antisense probes may be a good marker for the detection of ER-stressed OLs in jp mutant mice.

    DOI: 10.1007/s12565-016-0383-5

    Scopus

    PubMed

    researchmap

  • Risk factors of recurrence in pediatric congenital cholesteatoma 査読

    Yuka Morita, Kuniyuki Takahashi, Shuji Izumi, Yamato Kubota, Shinsuke Ohshima, Yutaka Yamamoto, Sugata Takahashi, Arata Horii

    Otology and Neurotology   38 ( 10 )   1463 - 1469   2017年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:Lippincott Williams and Wilkins  

    Objective: To examine the risk factors of recurrence in pediatric congenital cholesteatoma. Study Design: Retrospective chart review. Setting: University hospital. Patients: Sixty-seven patients having tympanic type of congenital cholesteatoma under 15-years old at surgery. Interventions: Canal wall-up tympanomastoidectomy (n=30) or transcanal atticotomy/tympanoplasty (n=37) was performed depending on cholesteatoma extension, 16 of which were followed by second-look surgery. Preoperative computed tomography (CT) before second-look surgery or follow-up CT was performed to detect residual recurrence 1 year after the surgery. Cholesteatoma found at the second surgery was also included in the recurrence. All patients had no recurrent cholesteatoma at the last follow-up (median, 61 mo after surgery). Main Outcome Measures: Possible predictive factors were compared between the groups. Results: Residual cholesteatoma and retraction cholesteatoma occurred in 21 and 6%, respectively. There was no significant difference in age, sex, and type of cholesteatoma (open or closed) between the groups
    however, Potsic stage and status of stapes involvement were more advanced in the residual cholesteatoma group. All residual lesions could be detected by follow-up CT or by second-look surgery. All of four retraction cholesteatoma patients were male, young at the surgery and in stage IV. Conclusion: Recurrence mostly occurred as residual cholesteatoma, suggesting that CT is recommended as a follow-up tool for congenital cholesteatoma. Advanced lesions had the risk of residual cholesteatoma, suggesting that complete removal of epithelium is important. Although rare, young advanced-stage patients had risk of retraction cholesteatoma and therefore normal mucosa should be preserved as much as possible for these patients.

    DOI: 10.1097/MAO.0000000000001587

    Scopus

    PubMed

    researchmap

  • Comprehensive microbiome analysis of tonsillar crypts in IgA nephropathy. 査読 国際誌

    Hirofumi Watanabe, Shin Goto, Hiroshi Mori, Koichi Higashi, Kazuyoshi Hosomichi, Naotaka Aizawa, Nao Takahashi, Masafumi Tsuchida, Yusuke Suzuki, Takuji Yamada, Arata Horii, Ituro Inoue, Ken Kurokawa, Ichiei Narita

    Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association   32 ( 12 )   2072 - 2079   2017年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background: Immunoglobulin A nephropathy (IgAN) is the most prevalent primary chronic glomerular disease, in which the mucosal immune response elicited particularly in the tonsils or intestine has been estimated to be involved in the development of the disease. To explore the relationship between IgAN and bacterial flora in the tonsils, we conducted a comprehensive microbiome analysis. Methods: We enrolled 48 IgAN patients, 21 recurrent tonsillitis (RT) patients without urine abnormalities and 30 children with tonsillar hyperplasia (TH) who had undergone tonsillectomy previously. Genomic DNA from tonsillar crypts of each patient was extracted, and V4 regions of the 16S ribosomal RNA gene were amplified and analysed using a high-throughput multiplexed sequencing approach. Differences in genus composition among the three study groups were statistically analysed by permutational multivariate analysis of variance and visualized by principal component analysis (PCA). Results: Substantial diversity in bacterial composition was detected in each sample. Prevotella spp., Fusobacterium spp., Sphingomonas spp. and Treponema spp. were predominant in IgAN patients. The percentage of abundance of Prevotella spp., Haemophilus spp., Porphyromonas spp. and Treponema spp. in IgAN patients was significantly different from that in TH patients. However, there was no significant difference in the percentage of abundance of any bacterial genus between IgAN and RT patients. PCA did not distinguish IgAN from RT, although it discriminated TH. No significant differences in microbiome composition among the groups of IgAN patients according to clinicopathological parameters were observed. Conclusions: Similar patterns of bacteria are present in tonsillar crypts of both IgAN and RT patients, suggesting that the host response to these bacteria might be important in the development of IgAN.

    DOI: 10.1093/ndt/gfw343

    PubMed

    researchmap

  • Bone density development of the temporal bone assessed by computed tomography 査読

    Kuniyuki Takahashi, Yuka Morita, Shinsuke Ohshima, Shuji Izumi, Yamato Kubota, Arata Horii

    Otology and Neurotology   38 ( 10 )   1445 - 1449   2017年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Lippincott Williams and Wilkins  

    Hypothesis: The temporal bone shows regional differences in bone development. Background: The spreading pattern of acute mastoiditis shows age-related differences. In infants, it spreads laterally and causes retroauricular swelling, whereas in older children, it tends to spread medially and causes intracranial complications. We hypothesized that bone maturation may influence the spreading pattern of acute mastoiditis. Methods: Eighty participants with normal hearing, aged 3 months to 42 years, participated in this study. Computed tomography (CT) values (Hounsfield unit [HU]) in various regions of the temporal bone, such as the otic capsule (OC), lateral surface of the mastoid cavity (LS), posterior cranial fossa (PCF), and middle cranial fossa (MCF), were measured as markers of bone density. Bone density development curves, wherein CT values were plotted against age, were created for each region. The age at which the CT value exceeded 1000 HU, which is used as an indicator of bone maturation, was calculated from the development curves and compared between the regions. Results: The OC showed mature bone at birth, whereas the LS, PCF, and MCF showed rapid maturation in early childhood. However, there were significant regional differences in the ages of maturation: 1.7, 3.9, and 10.8 years for the LS, PCF, and MCF, respectively. Conclusion: To our knowledge, this is the first report to show regional differences in the maturation of temporal bone, which could partly account for the differences in the spreading pattern of acute mastoiditis in individuals of different ages.

    DOI: 10.1097/MAO.0000000000001566

    Scopus

    PubMed

    researchmap

  • Evaluation of endolymphatic hydrops using 3-T MRI after intravenous gadolinium injection 査読

    Takao Imai, Atsuhiko Uno, Tadashi Kitahara, Tomoko Okumura, Arata Horii, Yumi Ohta, Takashi Sato, Suzuyo Okazaki, Takefumi Kamakura, Yoshiyuki Ozono, Yoshiyuki Watanabe, Yukiko Hanada, Ryusuke Imai, Kazuya Ohata, Hidenori Inohara

    EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY   274 ( 12 )   4103 - 4111   2017年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Aim of this work is to establish evaluation criteria for identifying endolymphatic hydrops in the vestibule and cochlea using a magnetic resonance imaging (MRI) scanner. This is a retrospective diagnostic study. We evaluated 70 ears of 35 unilateral Meniere's disease patients. We performed 3-T MRI 4 h after intravenous gadolinium injection. Otologists manually traced the outline of vestibule, cochlea, and endolymphatic space of the vestibule and cochlea on two-dimensional fluid-attenuated inversion-recovery (2D-FLAIR) images. The traced area was measured, and rates of endolymphatic space to the vestibule and cochlea were calculated. The same otologists judged whether the low signal intensity area of the cochlea was at the edge of the cochlea. For measuring the rate of endolymphatic space to the vestibule, when the cut-off value was 30%, the presence of endolymphatic hydrops was determined with sensitivity of 87.1% and specificity of 94.3%. In contrast, the rate of endolymphatic space to the cochlea produced low accuracy. Therefore, when the presence of endolymphatic hydrops in the cochlea was judged by whether the low signal intensity area in the cochlea was at the edge of cochlea, endolymphatic hydrops could be detected with sensitivity of 91.4% and specificity of 94.3%. We were able to identify endolymphatic hydrops in the vestibule when the rate of endolymphatic space to the vestibule was greater than 30%, and could detect endolymphatic hydrops in the cochlea when a low signal intensity area was located at the edge of the cochlea in 2D-FLAIR images.

    DOI: 10.1007/s00405-017-4739-9

    Web of Science

    PubMed

    researchmap

  • Microbiomes of the normal middle ear and ears with chronic otitis media 査読

    Shujiro B. Minami, Hideki Mutai, Tomoko Suzuki, Arata Horii, Naoki Oishi, Koichiro Wasano, Motoyasu Katsura, Fujinobu Tanaka, Tetsuya Takiguchi, Masato Fujii, Kimitaka Kaga

    Laryngoscope   127 ( 10 )   E371 - E377   2017年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:John Wiley and Sons Inc.  

    Objective: The aim of this study was to profile and compare the middle ear microbiomes of human subjects with and without chronic otitis media. Study Design: Prospective multicenter cohort study. Methods: All consecutive patients undergoing tympanoplasty surgery for chronic otitis media or ear surgery for conditions other than otitis media were recruited. Sterile swab samples were collected from the middle ear mucosa during surgery. The variable region 4 of the 16S rRNA gene in each sample were amplified using region-specific primers adapted for the Illumina MiSeq sequencer (Illumina, CA, USA)). The sequences were subjected to local blast and classified using Metagenome@KIN (World Fusion, Tokyo, Japan). Results: In total, 155 participants were recruited from seven medical centers. Of these, 88 and 67 had chronic otitis media and normal middle ears, respectively. The most abundant bacterial phyla on the mucosal surfaces of the normal middle ears were Proteobacteria, followed by Actinobacteria, Firmicutes, and Bacteroidetes. The children and adults with normal middle ears differed significantly in terms of middle ear microbiomes. Subjects with chronic otitis media without active inflammation (dry ear) had similar middle ear microbiomes as the normal middle ears group. Subjects with chronic otitis media with active inflammation (wet ear) had a lower prevalence of Proteobacteria and a higher prevalence of Firmicutes than the normal middle ears. Conclusion: The human middle ear is inhabited by more diverse microbial communities than was previously thought. Alteration of the middle ear microbiome may contribute to the pathogenesis of chronic otitis media with active inflammation. Level of Evidence: 2b. Laryngoscope, 127:E371–E377, 2017.

    DOI: 10.1002/lary.26579

    Scopus

    PubMed

    researchmap

  • Creating an Optimal 3D Printed Model for Temporal Bone Dissection Training. 査読

    Takahashi K, Morita Y, Ohshima S, Izumi S, Kubota Y, Yamamoto Y, Takahashi S, Horii A

    The Annals of otology, rhinology, and laryngology   3489417705395   2017年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/0003489417705395

    PubMed

    researchmap

  • Office-based differential diagnosis of transient and persistent geotropic positional nystagmus in patients with horizontal canal type of benign paroxysmal positional vertigo 査読

    Suzuyo Okazaki, Takao Imai, Kayoko Higashi-Shingai, Kazunori Matsuda, Noriaki Takeda, Tadashi Kitahara, Atsuhiko Uno, Arata Horii, Yumi Ohta, Tetsuo Morihana, Chisako Masumura, Suetaka Nishiike, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   137 ( 3 )   265 - 269   2017年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Conclusion: A 30s observation of geotropic positional nystagmus is sufficient to distinguish persistent geotropic positional nystagmus (PGPN) from transient geotropic positional nystagmus (TGPN) in patients with horizontal canal type of benign paroxysmal positional vertigo (H-BPPV) in ENT office.
    Objective: As a canalith repositioning procedure effectively treats H-BPPV with TGPN, but not PGPN, the differentiation between patients with PGPN and with TGPN is essential. The purpose of this study is to determine the observation period enough to distinguish TGPN from PGPN.
    Methods: This study first analyzed positional nystagmus images recorded with an infrared CCD camera three-dimensionally in 47 patients with H-BPPV. PGPN is distinguished from TGPN in patients with H-BPPV precisely by means of time constant calculated form analysis of positional nystagmus. Ten-second and 30-s movies were made of positional nystagmus of the all 47 patients. Ten independent otolaryngologists were then asked to distinguish TGPN from PGPN after a 10s or 30s observation of the geotropic positional nystagmus images in 47 patients with H-BPPV.
    Results: The sensitivity and specificity to distinguish TGPN from PGPN was 100% and 97% after 30s observation, but 100% and 40% after 10s observation, respectively.

    DOI: 10.1080/00016489.2016.1227478

    Web of Science

    PubMed

    researchmap

  • Vestibular Involvement in Patients With Otitis Media With Antineutrophil Cytoplasmic Antibody-associated Vasculitis 査読

    Yuka Morita, Kuniyuki Takahashi, Shuji Izumi, Yamato Kubota, Shinsuke Ohshima, Arata Horii

    OTOLOGY & NEUROTOLOGY   38 ( 1 )   97 - 101   2017年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Objective: Otitis media (OM) with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (OMAAV) is a novel concept of ear disease that is characterized by progressive mixed or sensorineural hearing loss with occasional systemic involvement. Considering the accumulating knowledge about the characteristics of and treatment for auditory dysfunction in OMAAV, the objective of this study was to investigate the vestibular function and symptoms of patients with OMAAV.
    Study Design: Retrospective chart review.
    Setting: University hospital.
    Patients: Thirty-one OMAAV patients met criteria proposed by the OMAAV study group in Japan.
    Main Outcome Measures: Clinical characteristics and vestibular tests.
    Results: Eleven of 31 OMAAV patients had vestibular symptoms; 3 patients had acute vertigo attack with sudden hearing loss and 8 patients had chronic dizziness. Episodic vertigo was not seen in any of the patients. Three patients who received a less intensive therapy without immunosuppressive agents developed intractable persistent dizziness. All symptomatic patients and six of the nine OMAAV patients without vestibular symptoms showed unilateral or bilateral caloric weakness; therefore, vestibular involvement was present in 84% of OMAAV patients. Gain of vestibuloocular reflex was reduced in symptomatic patients. The eye-tracking test and optokinetic nystagmus revealed no evidence of central dysfunction.
    Conclusion: Vestibular dysfunction was seen in 84% of OMAAV patients. One-third of OMAAV patients showed vestibular symptoms such as acute vertigo attack or chronic dizziness, which are of peripheral origin. One-third of the symptomatic patients developed intractable dizziness. Initial intensive treatment by combination therapy with steroid and immunosuppressive agents may be essential for preventing the development of intractable dizziness.

    DOI: 10.1097/MAO.0000000000001223

    Web of Science

    PubMed

    researchmap

  • Lower Airway Inflammation in Eosinophilic Chronic Rhinosinusitis as Determined by Exhaled Nitric Oxide 査読

    Rumi Kambara, Takafumi Minami, Hitoshi Akazawa, Fumio Tsuji, Takanobu Sasaki, Hidenori Inohara, Arata Horii

    INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY   173 ( 4 )   225 - 232   2017年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    Background: Chronic rhinosinusitis (CRS) is classified into eosinophilic CRS (ECRS) and non-ECRS. The objectives of this study were to evaluate lower airway inflammation by measuring the fractional concentration of exhaled nitric oxide (FeNO) and to examine the effects of endoscopic sinus surgery (ESS) on FeNO in patients with ECRS compared to non-ECRS. Methods: CRS patients with nasal polyps (23 with ECRS and 22 with non-ECRS) were enrolled into this study. ECRS was diagnosed based on the definition proposed by the Japanese Epidemiological Survey of Refractory Eosinophilic Chronic Rhinosinusitis (JESREC) study group. Several clinical markers including blood eosinophil counts, percent of eosinophils in white blood cells (WBC), number of eosinophils in nasal polyps, JESREC scores, total IgE, FeNO, and Lund-Mackay paranasal sinus CT scores were compared between ECRS and non-ECRS. These markers were also tested before and 2 months after ESS. Results: FeNO was significantly higher in patients with ECRS than in non-ECRS patients. When all CRS patients were tested, a significant correlation was found between FeNO and eosinophilic markers including blood eosinophil counts, percent of eosinophils in WBC, number of eosinophils in nasal polyps, and JESREC scores. FeNO showed a significant correlation with Lund-Mackay scores only in ECRS patients. Blood eosinophil counts, percent of eosinophils in WBC, and FeNO decreased after ESS only in ECRS patients. Conclusions: ECRS patients had lower airway inflammation as revealed by an elevated FeNO, which was parallel to the Lund-Mackay CT scores. ESS decreased the blood eosinophils and FeNO, leading to an improvement of the occult pulmonary dysfunction in ECRS patients. (C) 2017 S. Karger AG, Basel

    DOI: 10.1159/000479387

    Web of Science

    PubMed

    researchmap

  • Diagnostic criteria for persistent postural-perceptual dizziness (PPPD): Consensus document of the committee for the Classification of Vestibular Disorders of the Bárány Society. 査読

    Staab JP, Eckhardt-Henn A, Horii A, Jacob R, Strupp M, Brandt T, Bronstein A

    Journal of vestibular research : equilibrium & orientation   27 ( 4 )   191 - 208   2017年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3233/VES-170622

    Web of Science

    PubMed

    researchmap

  • Three-dimensional reconstruction of root cells and interdental cells in the rat inner ear by serial section scanning electron microscopy 査読

    Ryusuke Shodo, Manabu Hayatsu, Daisuke Koga, Arata Horii, Tatsuo Ushiki

    BIOMEDICAL RESEARCH-TOKYO   38 ( 4 )   239 - 248   2017年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    In the cochlea, a high K+ environment in the endolymph is essential for the maintenance of normal hearing function, and the transport of K+ ions through gap junctions of the cochlear epithelium is thought to play an important role in endolymphatic homeostasis. The aim of the present study was to demonstrate the three-dimensional (3D) ultrastructure of spiral ligament root cells and interdental cells, which are located at both ends of the gap junction system of the cochlea epithelium. Serial semi-thin sections of plastic-embedded rat cochlea were mounted on glass slides, stained with uranyl acetate and lead citrate, and observed by scanning electron microscopy (SEM) using the backscattered electron (BSE) mode. 3D reconstruction of BSE images of serial sections revealed that the root cells were linked together to form a branched structure like an elaborate "tree root" in the spiral ligament. The interdental cells were also connected to each other, forming a comb-shaped cellular network with a number of cellular strands in the spiral limbus. Furthermore, TEM studies of ultra-thin sections revealed the rich presence of gap junctions in both root cells and interdental cells. These findings suggest the possibility that both root cells and interdental cells contribute to K+ circulation as the end portion of the epithelial cell gap junction system of the cochlea.

    DOI: 10.2220/biomedres.38.239

    Web of Science

    PubMed

    researchmap

  • Hippocampal gene expression, serum cortisol level, and spatial memory in rats exposed to hypergravity 査読

    Arata Horii, Kenji Mitani, Chisako Masumura, Atsuhiko Uno, Takao Imai, Yuka Morita, Kuniyuki Takahashi, Tadashi Kitahara, Hidenori Inohara

    JOURNAL OF VESTIBULAR RESEARCH-EQUILIBRIUM & ORIENTATION   27 ( 4 )   209 - 215   2017年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IOS PRESS  

    BACKGROUND: Due to spatial disorientation reported in space, spatial memory and navigation performances could be more largely impaired by gravity changes. Hippocampus, a key structure for spatial memory, receives inputs from gravity-sensing otolith organs.
    OBJECTIVE: To determine the key molecules in the rat hippocampus that contribute to an adaptation to altered gravity in terms of spatial memory performance.
    METHODS: Gene expression of hippocampus and spatial memory after continuous two-weeks exposure to 2G hypergravity (HG) were examined using a microarray analysis followed by real-time PCR methods and radial arm maze testing, respectively. Serum cortisol levels during HG load were measured as a stress marker.
    RESULTS: Accuracy to enter the correct arms in HG rats was significantly lower than that of controls, indicating an impaired spatial memory due to gravity changes. Microarray analysis followed by real-time PCR confirmed an upregulation of insulin like growth factor binding protein 2 (IGFBP2) gene. Serum cortisol level was the same level as controls at the last day of hypergravity, suggesting the adaptation to HG-induced stress.
    CONCLUSIONS: Given that the IGF systems are involved in neurotrophic and synaptic plasticity mechanisms, IGF system might contribute to the adaptation to altered gravity in terms of spatial memory.

    DOI: 10.3233/VES-170521

    Web of Science

    PubMed

    researchmap

  • Effects of Covering Surgical Wounds with Polyglycolic Acid Sheets for Posttonsillectomy Pain 査読

    Shin-ichi Miyaguchi, Arata Horii, Rumi Kambara, Norihiko Takemoto, Hitoshi Akazawa, Nao Takahashi, Hironori Baba, Hidenori Inohara

    OTOLARYNGOLOGY-HEAD AND NECK SURGERY   155 ( 5 )   876 - 878   2016年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS LTD  

    Postoperative pain is a remaining issue in tonsillectomy. Polyglycolic acid (PGA) is a biocompatible material used for absorbent suture reinforcement, and its sheet has been applied for covering defects after resection of oral carcinoma. The aim of this study is to examine whether the attachment of a PGA sheet to surgical wounds would reduce posttonsillectomy pain. In this prospective single-blind study, 17 consecutive adult patients were recruited who needed to undergo tonsillectomy, mainly due to habitual tonsillitis. Following bilateral tonsillectomies, a PGA sheet was attached with fibrin glue to only 1 side, without notification to patients of which side. Postoperative pain of each side was separately evaluated with a visual analog scale at 4 time points: before each meal and before sleep. Postoperative pain of both the PGA sheet-attached and nonattached sides was most severe before breakfast among 4 time points. Postoperative pain measured before breakfast was significantly more severe in the PGA sheet-attached side than the nonattached side. As such, this study provided solid data on the negative effects of PGA sheeting on posttonsillectomy pain.

    DOI: 10.1177/0194599816660072

    Web of Science

    PubMed

    researchmap

  • Auditory cortical field coding long-lasting tonal offsets in mice 査読

    Hironori Baba, Hiroaki Tsukano, Ryuichi Hishida, Kuniyuki Takahashi, Arata Horii, Sugata Takahashi, Katsuei Shibuki

    SCIENTIFIC REPORTS   6   34421   2016年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Although temporal information processing is important in auditory perception, the mechanisms for coding tonal offsets are unknown. We investigated cortical responses elicited at the offset of tonal stimuli using flavoprotein fluorescence imaging in mice. Off-responses were clearly observed at the offset of tonal stimuli lasting for 7 s, but not after stimuli lasting for 1 s. Off-responses to the short stimuli appeared in a similar cortical region, when conditioning tonal stimuli lasting for 5-20 s preceded the stimuli. MK-801, an inhibitor of NMDA receptors, suppressed the two types of off-responses, suggesting that disinhibition produced by NMDA receptor-dependent synaptic depression might be involved in the off-responses. The peak off-responses were localized in a small region adjacent to the primary auditory cortex, and no frequency-dependent shift of the response peaks was found. Frequency matching of preceding tonal stimuli with short test stimuli was not required for inducing off-responses to short stimuli. Two-photon calcium imaging demonstrated significantly larger neuronal off-responses to stimuli lasting for 7 s in this field, compared with off-responses to stimuli lasting for 1 s. The present results indicate the presence of an auditory cortical field responding to long-lasting tonal offsets, possibly for temporal information processing.

    DOI: 10.1038/srep34421

    Web of Science

    PubMed

    researchmap

  • A Survey of the Otorhinolaryngological Screening of Children in Niigata Prefecture: Kindergarten to High School. 査読

    Ohtaki H, Hirokawa T, Ishioka K, Horii A, Takahashi S

    Nihon Jibiinkoka Gakkai kaiho   119 ( 7 )   941 - 8   2016年7月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    PubMed

    researchmap

  • [A Case of Cystic Cervical Lymph Node Metastasis of HPV-positive Tonsil Cancer, Being Discriminated as the Branchiogenic Carcinoma]. 査読

    Kambara R, Tamai M, Horii A

    Nihon Jibiinkoka Gakkai kaiho   119 ( 2 )   118 - 24   2016年2月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    PubMed

    researchmap

  • Functional Expression of an Osmosensitive Cation Channel, Transient Receptor Potential Vanilloid 4, in Rat Vestibular Ganglia 査読

    Takefumi Kamakura, Makoto Kondo, Yoshihisa Koyama, Yukiko Hanada, Yusuke Ishida, Yukiko Nakamura, Takahiro Yamada, Yasumitsu Takimoto, Tadashi Kitahara, Yoshiyuki Ozono, Arata Horii, Takao Imai, Hidenori Inohara, Shoichi Shimada

    AUDIOLOGY AND NEURO-OTOLOGY   21 ( 4 )   268 - 274   2016年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    Transient receptor potential vanilloid (TRPV) 4 is a nonselective cation channel expressed in sensory neurons such as those in the dorsal root and trigeminal ganglia, kidney, and inner ear. TRPV4 is activated by mechanical stress, heat, low osmotic pressure, low pH, and phorbol derivatives such as 4 alpha-phorbol 12,13-didecanoate (4 alpha-PDD). We investigated the expression of TRPV4 in rat vestibular ganglion (VG) neurons. The TRPV4 gene was successfully amplified from VG neuron mRNA using reverse-transcription polymerase chain reaction. Furthermore, immunoblotting showed positive expression of TRPV4 protein in VG neurons. Immunohistochemistry indicated that TRPV4 was localized predominantly on the plasma membrane of VG neurons. Calcium (Ca2+) imaging of VG neurons showed that 4 alpha-PDD and/or hypotonic stimuli caused an increase in intracellular Ca2+ concentration ([Ca2+](i)) that was almost completely inhibited by ruthenium red, a selective antagonist of TRPV channels. Interestingly, a [Ca2+](i) increase was evoked by both hypotonic stimuli and 4 alpha-PDD in approximately 38% of VG neurons. These data indicate that TRPV4 is functionally expressed in VG neurons as an ion channel and that TRPV4 likely participates in VG neurons for vestibular neurotransmission as an osmoreceptor and/or mechanoreceptor. (C) 2016 S. Karger AG, Basel

    DOI: 10.1159/000449238

    Web of Science

    PubMed

    researchmap

  • Psychiatric comorbidities and use of milnacipran in patients with chronic dizziness 査読

    Arata Horii, Takao Imai, Tadashi Kitahara, Atsuhiko Uno, Yuka Morita, Kuniyuki Takahashi, Hidenori Inohara

    JOURNAL OF VESTIBULAR RESEARCH-EQUILIBRIUM & ORIENTATION   26 ( 3 )   335 - 340   2016年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IOS PRESS  

    BACKGROUND: Psychiatric comorbidities are an important issue in the treatment of chronic dizziness patients.
    OBJECTIVE: To test the correlation between psychiatric status and subjective handicaps and to examine the effects of milnacipran on handicaps.
    METHODS: Hospital anxiety and depression scale (HADS) and handicaps were assessed by a questionnaire before and eight weeks after milnacipran treatment (50 mg/day) in 29 consecutive patients with chronic dizziness. Effects of milnaciplan were compared with fluvoxamine (200 mg/day).
    RESULTS: A significant correlation was found between anxious and depressive scale scores and also between HADS and handicaps. Duration of symptomswas longer in the anxious/depressive group( HADS >= 13) than in the non-anxious/depressive group. Handicaps and HADS were significantly decreased after treatment only in the anxious/depressive group. There were no overall differences in drug effects between milnaciplan and fluvoxamine. However, the rate of patients with a post/pre ratio of handicaps <80% was higher in milnaciplan group compared with the fluvoxamine group.
    CONCLUSIONS: Not only anxiety disorders but also depression should be considered as comorbid psychiatric disorders in patients with chronic dizziness. Dizzy patients with psychiatric comorbidities have a longer duration of symptoms and more handicaps than those without psychiatric disorders. Milnacipran may be chosen as a treatment for patients with chronic dizziness with comorbid psychiatric disorders in case of and insufficient response to SSRIs.

    DOI: 10.3233/VES-160582

    Web of Science

    PubMed

    researchmap

  • Prognostic predictors of sudden sensorineural hearing loss in defibrinogenation therapy. 査読

    Oya R, Horii A, Akazawa H, Osaki Y, Inohara H

    Acta oto-laryngologica   1 - 6   2015年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3109/00016489.2015.1104723

    PubMed

    researchmap

  • [A Case of the Relapsing Polychondritis Limited to the Trachea]. 査読

    Mori K, Ishioka K, Yamazaki H, Ueki Y, Kubota Y, Matsuyama H, Yamamoto Y, Horii A, Takahashi S

    Nihon Jibiinkoka Gakkai kaiho   118 ( 9 )   1150 - 4   2015年9月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    PubMed

    researchmap

  • [A Case of Intravascular Lymphoma Mimicking Acute Sinusitis in Which the Outcome was Unfortunate]. 査読

    Kambara R, Horii A, Osak Y, Inohara H

    Nihon Jibiinkoka Gakkai kaiho   118 ( 6 )   770 - 5   2015年6月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    PubMed

    researchmap

  • CD16(+)CD56(+) cells are a potential culprit for hematuria in IgA nephropathy 査読

    Hirotsugu Iwatani, Yasuyuki Nagasawa, Ryohei Yamamoto, Kenichiro Iio, Masayuki Mizui, Arata Horii, Tadashi Kitahara, Hidenori Inohara, Atsushi Kumanogoh, Enyu Imai, Hiromi Rakugi, Yoshitaka Isaka

    CLINICAL AND EXPERIMENTAL NEPHROLOGY   19 ( 2 )   216 - 224   2015年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Hematuria is the first manifestation of urinary abnormality in immunoglobulin A nephropathy (IgAN). Hematuria has recently been reported as a risk factor for deterioration of renal function; however, its cause remains unknown.
    We analyzed the surface marker of peripheral blood mononuclear cells before and immediately after tonsillectomy in IgAN patients and controls (chronic tonsillitis or tonsillar hypertrophy) by flow cytometry and investigated the association with hematuria. To prove our hypothesis that NK cells induce hematuria, we administered IL-12, activator of NK cells, to HIGA mice. In addition, we transferred cultured NK cells to nude rats and transferred the CD16(+)CD56(+) cells, including NK cells, that are derived from the peripheral blood of IgAN patients immediately after tonsillectomy to nude rats to assess the hematuria level and renal histology of the recipients. We also performed cytotoxicity assays against glomerular endothelial cells by NK cells.
    We found that IgAN patients who showed rapid deterioration of hematuria after tonsillectomy also displayed a significant increase in CD16(+)CD56(+) cells in the peripheral blood immediately after tonsillectomy. Exogenous administration of IL-12 to HIGA mice induced hematuria. Adoptive transfer of either cells of an NK cell line, or of CD16(+)CD56(+) cells derived from IgAN patients, into nude rats induced hematuria in the recipients. In vitro analysis showed that NK cells exert cytotoxic activity toward human glomerular endothelial cells in a dose-dependent manner.
    CD16(+)CD56(+) cells seem to be responsible for hematuria in IgAN.

    DOI: 10.1007/s10157-014-0968-z

    Web of Science

    PubMed

    researchmap

  • Light cupula: the pathophysiological basis of persistent geotropic positional nystagmus 査読

    Takao Imai, Kazunori Matsuda, Noriaki Takeda, Atsuhiko Uno, Tadashi Kitahara, Arata Horii, Suetaka Nishiike, Hidenori Inohara

    BMJ OPEN   5 ( 1 )   e006607   2015年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BMJ PUBLISHING GROUP  

    Objective: To clarify the pathophysiological basis of persistent geotropic positional nystagmus (PGN) in patients with the horizontal canal type of benign paroxysmal positional vertigo (H-BPPV), the time constant (TC) of nystagmus and the relationship between its slow phase eye velocity (SPV) and the angle of head rotation in supine were defined.
    Methods: Geotropic or apogeotropic positional nystagmus was recorded by video-oculography and analysed three-dimensionally.
    Results: Geotropic positional nystagmuses in patients with H-BPPV were classified as transient geotropic positional nystagmus with a TC of <= 35 s or PGN with a TC of >35 s. Alternatively, the TC of persistent apogeotropic positional nystagmus (AN) in patients with H-BPPV was >35 s. The direction of the SPV of patients with PGN was opposite to that of patients with AN at each head position across the range of neutral head positions. The relationship between the SPV of patients with PGN and the angle of head rotation was linearly symmetrical against that of patients with AN with respect to a line drawn on the neutral head position.
    Conclusions: Since its TC was >35 s, it is suggested that PGN is induced by cupula deviation in response to gravity at each head position. It is also suggested that the direction of cupula deviation in patients with PGN is opposite to that of patients with AN across the neutral head positional range with no nystagmus where the long axis of cupula is in alignment with the axis of gravity. Since the pathophysiological basis of AN is considered a heavy cupula, it is suggested that PGN is conversely induced by a light cupula.

    DOI: 10.1136/bmjopen-2014-006607

    Web of Science

    PubMed

    researchmap

  • Somatosensory shift of postural control in dizzy patients 査読

    Tomoko Okumura, Arata Horii, Tadashi Kitahara, Takao Imai, Atsuhiko Uno, Yasuhiro Osaki, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   135 ( 9 )   925 - 930   2015年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Conclusions: Postural control is dependent on the visual system in normal conditions. Shift from visual to somatosensory dependence in dizzy patients suggests that utilizing the stable visual references is recommended for the rehabilitation of dizzy patients. Objectives: To investigate which of the visual or somatosensory system is mainly used for substitution of the impaired spatial orientation in dizzy patients. Methods: We recruited 189 consecutive patients with or without dizziness and vestibular dysfunction. Dizzy patients were divided into three groups: acute, episodic, and chronic dizziness. Vestibular function was assessed by caloric test, traditional head impulse test, and head shaking nystagmus. Visual or somatosensory dependence of spatial orientation was assessed by posturography on a solid surface or on foam in eyes open or closed condition. The foam ratio (posturography with/without foam) when eyes were closed was indicative of somatosensory dependence of postural control, whereas the Romberg ratio on foam showed visual dependence. (Romberg ratio on foam)/(foam ratio with eyes closed) was calculated and used as an index of the visual/somatosensory dependence of postural control. Results: The visual/somatosensory ratio of postural control was significantly lower in dizzy patients as well as patients with vestibular dysfunction, however, no differences were found between acute, episodic, and chronic dizziness.

    DOI: 10.3109/00016489.2015.1040172

    Web of Science

    PubMed

    researchmap

  • Gadolinium contrast-enhanced MRI reveals cystic lateral semicircular canal contents 査読

    Kayoko Higashi-Shingai, Takao Imai, Yasumitsu Takimoto, Tomoko Okumura, Yumi Ohta, Tetsuo Morihana, Atsuhiko Uno, Yoshiyuki Watanabe, Arata Horii, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   135 ( 10 )   1000 - 1006   2015年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Conclusion: Contrast-enhanced magnetic resonance imaging (MRI) reveals variations in the endolymphatic morphology of the cystic lateral semicircular canal (CLSC) that correlate with inner ear function. This report is the first to suggest a relationship between the morphology and function of this common inner ear malformation in clinical cases. Objectives: This study investigated the radiological and functional findings of a common inner ear malformation using computed tomography (CT), gadolinium contrast-enhanced magnetic resonance imaging (MRI), caloric testing, and cervical and ocular vestibular evoked myogenic potential (VEMP) testing. Method: Four ears in three patients who were radiologically diagnosed with a CLSC and a normal cochlea on high-resolution CT and contrast-enhanced MRI were included. Semicircular canal and vestibular functions were analyzed using the caloric test and cervical and ocular VEMP testing. Results: Unilateral and bilateral cystic canals were found in two and one patients, respectively. In the first patient, the malformed vestibule and cystic space were separate on imaging, and perilymph filled the cystic space. The functional test results were normal. In the second patient, endolymph filled both cystic spaces, and the functional responses were poor. In the third patient, endolymph filled the cystic space, and the ear did not respond during functional testing.

    DOI: 10.3109/00016489.2015.1043143

    Web of Science

    PubMed

    researchmap

  • Effects of endolymphatic sac decompression surgery on vertigo and hearing in patients with bilateral Ménière's disease. 査読

    Kitahara T, Horii A, Imai T, Ohta Y, Morihana T, Inohara H, Sakagami M

    Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology   35 ( 10 )   1852 - 7   2014年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1097/MAO.0000000000000469

    PubMed

    researchmap

  • Effects of Endolymphatic Sac Decompression Surgery on Vertigo and Hearing in Patients With Bilateral Meniere's Disease

    Tadashi Kitahara, Arata Horii, Takao Imai, Yumi Ohta, Tetsuo Morihana, Hidenori Inohara, Masafumi Sakagami

    OTOLOGY & NEUROTOLOGY   35 ( 10 )   1852 - 1857   2014年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Objective: The aim of the present study, which involved a 2-year observation period and a nonsurgical control group, was to determine whether endolymphatic sac decompression surgery (ESDS) has the potential to prevent the progression of vertigo and hearing loss in patients with intractable bilateral Meniere's disease (MD).
    Study Design: Prospective case-control study.
    Setting: Tertiary referral center.
    Methods: Between 1996 and 2008, we subjected 67 patients with intractable bilateral MD to ESDS and local corticosteroid treatment. Another 34 patients with intractable bilateral MD who declined ESDS were treated with the best available medical therapies. All of the patients underwent regular follow-up examinations for at least 2 years.
    Results: Vertigo was resolved in 22 of 34 patients in the non-ESDS group and 60 of 67 patients in the ESDS group (p = 0.055, Fisher's exact test). Of the 24 patients in the non-ESDS group and 55 patients in the ESDS group in whom the ipsilateral ear (the treated ear) exhibited worse hearing function than the contralateral ear, the hearing level of the former ear was preserved in 13 and 52 patients, respectively (p = 0.007, Fisher's exact test). Of the 10 patients in the nonsurgical group and 12 patients in the surgical group in whom the ipsilateral ear exhibited better hearing function than the contralateral ear, the hearing level of the former ear was preserved in 2 and 11 patients, respectively (p = 0.035, Fisher's exact test).
    Conclusion: The present findings suggest that ESDS combined with local corticosteroid treatment can control progressive hearing loss in both ears in patients with bilateral MD at least during the first 2 postoperative years.

    Web of Science

    researchmap

  • Long-term prognosis of hearing loss in patients with unilateral Ménière's disease. 査読

    Sato G, Sekine K, Matsuda K, Ueeda H, Horii A, Nishiike S, Kitahara T, Uno A, Imai T, Inohara H, Takeda N

    Acta oto-laryngologica   134 ( 10 )   1005 - 10   2014年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3109/00016489.2014.923114

    PubMed

    researchmap

  • Does endolymphatic sac decompression surgery prevent bilateral development of unilateral Ménière disease? 査読

    Kitahara T, Horii A, Imai T, Ohta Y, Morihana T, Inohara H, Sakagami M

    The Laryngoscope   124 ( 8 )   1932 - 6   2014年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/lary.24614

    PubMed

    researchmap

  • Chronic Otitis Media With Cholesteatoma With Canal Fistula and Bone Conduction Threshold After Tympanoplasty With Mastoidectomy 査読

    Tadashi Kitahara, Takefumi Kamakura, Yumi Ohta, Tetsuo Morihana, Arata Horii, Atsuhiko Uno, Takao Imai, Yasuo Mishiro, Hidenori Inohara

    OTOLOGY & NEUROTOLOGY   35 ( 6 )   981 - 988   2014年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Objective: To understand the third mobile window effect of chronic otitis media with cholesteatoma with inner ear fistula on the bone conduction threshold, we examined changes in the bone conduction audiogram after tympanoplasty with mastoidectomy for chronic otitis media with cholesteatoma with canal fistula.
    Study Design: Retrospective case review.
    Setting: Tertiary referral center. Patients: According to the intraoperative classification of Dornhoffer and Milewski, we focused especially on Type IIa (anatomic bony fistula with no perilymph leak). We checked the bone conduction threshold at least 3 times: just before, just after, and 6 months after surgery in 20 ears with Type IIa lateral semicircular canal fistula. Intervention: Tympanoplasty with mastoidectomy.
    Main Outcome Measure: Bone conduction thresholds before and after tympanoplasty with mastoidectomy.
    Results: Compared with the preoperative bone conduction threshold, 6 cases were better, 12 cases were unchanged, and 2 cases were worse within the first postoperative week. Finally, 1 case was better, 15 cases were unchanged, and 4 cases were worse at the sixth postoperative month. Patients with a better bone conduction threshold in the low-tone frequencies immediately after surgery had a tendency to show no preoperative fistula symptoms. Postoperative spontaneous nystagmus had a tendency to be observed in patients with a worse bone conduction threshold in the high-tone frequencies.
    Conclusion: The better bone conduction threshold at low-tone frequencies immediately after tympanoplasty with mastoidectomy and no preoperative fistula symptoms might imply the third mobile window theory. The worse bone conduction threshold in high-tone frequencies with spontaneous nystagmus after surgery might indicate inner ear damage.

    DOI: 10.1097/MAO.0000000000000306

    Web of Science

    researchmap

  • Chronic otitis media with cholesteatoma with canal fistula and bone conduction threshold after tympanoplasty with mastoidectomy. 査読

    Kitahara T, Kamakura T, Ohta Y, Morihana T, Horii A, Uno A, Imai T, Mishiro Y, Inohara H

    Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology   35 ( 6 )   981 - 8   2014年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1097/MAO.0000000000000306

    PubMed

    researchmap

  • 5-HT3 receptor expression in the mouse vestibular ganglion 査読

    Yasumitsu Takimoto, Yusuke Ishida, Yukiko Nakamura, Takefurni Kamakura, Takahiro Yamada, Makoto Kondo, Tadashi Kitahara, Atsuhiko Uno, Takao Imai, Arata Horii, Suzuyo Okazaki, Suetaka Nishiike, Hidenori Inohara, Shoichi Shimada

    BRAIN RESEARCH   1557   74 - 82   2014年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The 5-hydroxytryptamine type 3 (5-HT3) receptor is a ligand-gated ion channel and a member of the Cys-loop family of receptors. Previous studies have shown 5-HT3 receptor expression in various neural cells of the central and peripheral nervous systems. Although the function and distribution of the 5-HT3 receptor has been well established, its role in the inner ear is still poorly understood. Moreover, no study has yet determined its localization and function in the peripheral vestibular nervous system. In the present study, we reveal mRNA expression of both 5-HT3A and 5-HT3B receptor subunits in the mouse vestibular ganglion (VG) by RT-PCR and in situ hybridization (ISH). We also show by ISH that 5-HT3 receptor mFtNA is only expressed in the VG (superior and inferior division) in the peripheral vestibular nervous system. Moreover, we performed Ca2+ imaging to determine whether functional 5-HT3 receptors are present in the mouse VG, using a selective 5-HT3 receptor agonist, SR57227A. In wild mice, 32% of VG neurons responded to the agonist, whereas there was no response in 5-HT3A receptor knockout mice. These results indicate that VG cells express functional 5-HT3 receptor channels and might play a modulatory role in the peripheral vestibular nervous system. (C) 2014 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.brainres.2014.02.016

    Web of Science

    PubMed

    researchmap

  • Periodontal Disease Bacteria Specific to Tonsil in IgA Nephropathy Patients Predicts the Remission by the Treatment 査読

    Yasuyuki Nagasawa, Kenichiro Iio, Shinji Fukuda, Yasuhiro Date, Hirotsugu Iwatani, Ryohei Yamamoto, Arata Horii, Hidenori Inohara, Enyu Imai, Takeshi Nakanishi, Hiroshi Ohno, Hiromi Rakugi, Yoshitaka Isaka

    PLOS ONE   9 ( 1 )   e81636   2014年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Background: Immunoglobulin (Ig) A nephropathy (IgAN) is the most common form of primary glomerulonephritis in the world. Some bacteria were reported to be the candidate of the antigen or the pathogenesis of IgAN, but systematic analysis of bacterial flora in tonsil with IgAN has not been reported. Moreover, these bacteria specific to IgAN might be candidate for the indicator which can predict the remission of IgAN treated by the combination of tonsillectomy and steroid pulse.
    Methods and Findings: We made a comprehensive analysis of tonsil flora in 68 IgAN patients and 28 control patients using Denaturing gradient gel electrophoresis methods. We also analyzed the relationship between several bacteria specific to the IgAN and the prognosis of the IgAN. Treponema sp. were identified in 24% IgAN patients, while in 7% control patients (P = 0.062). Haemophilus segnis were detected in 53% IgAN patients, while in 25% control patients (P = 0.012). Campylobacter rectus were identified in 49% IgAN patients, while in 14% control patients (P = 0.002). Multiple Cox proportional-hazards model revealed that Treponema sp. or Campylobactor rectus are significant for the remission of proteinuria (Hazard ratio 2.35, p = 0.019). There was significant difference in remission rates between IgAN patients with Treponema sp. and those without the bacterium (p = 0.046), and in remission rates between IgAN patients with Campylobacter rectus and those without the bacterium (p = 0.037) by Kaplan-Meier analysis. Those bacteria are well known to be related with the periodontal disease. Periodontal bacteria has known to cause immune reaction and many diseases, and also might cause IgA nephropathy.
    Conclusion: This insight into IgAN might be useful for diagnosis of the IgAN patients and the decision of treatment of IgAN.

    DOI: 10.1371/journal.pone.0081636

    Web of Science

    PubMed

    researchmap

  • Effects of endolymphatic sac decompression surgery on endolymphatic hydrops 査読

    Munehisa Fukushima, Tadashi Kitahara, Arata Horii, Hidenori Inohara

    Acta Oto-Laryngologica   133 ( 12 )   1292 - 1296   2013年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Conclusions: The present findings suggest that complete control of vertigo after endolymphatic sac decompression surgery (ESDS) does not always depend on improved vestibular function or reduced endolymphatic hydrops. Vertigo control is, however, associated with hearing stability. Objective: Among surgical treatments for intractable Meniere's disease, ESDS is performed to preserve and improve inner ear function. We examined the correlation between changes in vertigo frequency and neuro-otologic function to understand the condition of the inner ear in patients whose vertigo was completely controlled after undergoing ESDS. Methods: This was a retrospective cross-tabulation study. Between 1997 and 2001, we treated 52 patients with intractable vertigo using ESDS and followed the patients regularly for 2 years. Postoperatively we evaluated and recorded changes in vertigo attack frequency, maximum slow phase eye velocity, worst hearing level, and glycerol test results according to modified American Academy of Otolaryngology-Head and Neck Surgery 1995 criteria. Results: We found no correlation between vertigo control and vestibular function. There was also no correlation between vertigo control and negative conversion of the glycerol test. There was a significant correlation between vertigo control and hearing control . © 2013 Informa Healthcare.

    DOI: 10.3109/00016489.2013.831480

    Scopus

    PubMed

    researchmap

  • Long-term results of endolymphatic sac drainage with local steroids for intractable Meniere's disease 査読

    Tadashi Kitahara, Munehisa Fukushima, Atsuhiko Uno, Takao Imai, Yumi Ohta, Tetsuo Morihana, Takefumi Kamakura, Arata Horii, Hidenori Inohara

    Auris Nasus Larynx   40 ( 5 )   425 - 430   2013年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Objectives: Meniere's disease is a common inner ear disease characterized by vertigo, hearing loss and tinnitus. Since Meniere's disease is thought to be triggered by an immune insult to inner ear hydrops, we examined endolymphatic sac drainage with intra-endolymphatic sac application of large doses of steroids for intractable Meniere's patients and observed long-term results from 2 years to over a decade until 13 years. Methods: Between 1998 and 2009, we enrolled and assigned 286 intractable Meniere's patients to two groups: group-I (G-I) included patients who underwent endolymphatic sac drainage with steroid instillation and group-II (G-II) included those who declined endolymphatic sac drainage. Definitive spells and hearing improvement in these two groups were determined for 2-13 years after treatment. Results: According to the established criteria, vertigo was completely controlled in 88% of patients in G-I in the 2nd year, in 73% in the 12th year and in 70% in the 13th year. These results in G-I were significantly better than those in G-II for 13 years after treatment. Hearing was improved in 49% of patients in G-I in the 2nd year, in 27% in the 12th year and in 25% in the 13th year. These results in G-I were significantly better than those in G-II for 12 years after treatment, but this was not significant in the 13th year. Conclusions: Endolymphatic sac drainage with intra-endolymphatic sac application of large doses of steroids could improve long-term follow-up results of hearing as well as vertigo control. This means that the drainage with local steroids could also improve patients' long-term quality in the prime of life. © 2012 Elsevier Ireland Ltd.

    DOI: 10.1016/j.anl.2012.11.008

    Scopus

    PubMed

    researchmap

  • Changes in endolymphatic hydrops after sac surgery examined by Gd-enhanced MRI 査読

    Atsuhiko Uno, Takao Imai, Yoshiyuki Watanabe, Hisashi Tanaka, Tadashi Kitahara, Arata Horii, Takefumi Kamakura, Yasumitsu Takimoto, Yasuhiro Osaki, Suetaka Nishiike, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   133 ( 9 )   924 - 929   2013年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INFORMA HEALTHCARE  

    Conclusion: Endolymphatic hydrops could be a reversible inner ear pathological condition. After sac surgery, hydrops was reduced and symptoms went into remission in some cases, although vertigo suppression was not always a result of the reduced hydrops. Objective: To examine the changes in endolymphatic hydrops detected by gadolinium (Gd) contrast-enhanced magnetic resonance imaging (MRI) before and 6 months after endolymphatic sac surgery in patients with unilateral Meniere's disease. Methods: Fluid-attenuated inversion recovery MRI was obtained 4 h after intravenous administration or 24 h after intratympanic administration of Gd contrast medium. An enlarged negative stain corresponding to the cochlear duct and endolymphatic space of the vestibule was assessed as hydrops. Results: Of seven patients with hydrops confirmed by MRI before surgery, both cochlear and vestibular hydrops became negative in two, cochlear hydrops became negative in one, both hydrops were present, but reduced, in one, and there was no change in three patients. The number of vertigo spells was reduced in all cases at 6-12 months after surgery. As for the three cases of negative hydrops, vertigo was completely suppressed. In two cases in which hearing level improved, hydrops became negative after surgery.

    DOI: 10.3109/00016489.2013.795290

    Web of Science

    PubMed

    researchmap

  • Functional expression of TRPV1 and TRPA1 in rat vestibular ganglia 査読

    Takefumi Kamakura, Yusuke Ishida, Yukiko Nakamura, Takahiro Yamada, Tadashi Kitahara, Yasumitsu Takimoto, Arata Horii, Atsuhiko Uno, Takao Imai, Suzuyo Okazaki, Hidenori Inohara, Shoichi Shimada

    NEUROSCIENCE LETTERS   552   92 - 97   2013年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Both TRPV1 and TRPA1 are non-selective cation channels. They are co-expressed, and interact in sensory neurons such as dorsal root ganglia (DRG) and trigeminal ganglia (TG), and are involved in nociception, being activated by nociceptive stimuli. Immunohistological localization of TRPV1 in vestibular ganglion (VG) neurons has been reported. Although TRPA1 is co-expressed with TRPV1 in DRG and TG neurons, it is unclear whether TRPA1 channels are expressed in VG neurons. Moreover, it is unknown whether TRPV1 and TRPA1 channels are functional in VG neurons. We investigated the expression of TRPV1 and TRPA1 in rat VG neurons by RT-PCR, in situ hybridization, immunohistochemistry, and Ca2+ imaging experiments. Both TRPV1 and TRPA1 RT-PCR products were amplified from the mRNA of rat VG neurons. In situ hybridization experiments showed TRPV1 and TRPA1 mRNA expression in the majority of VG neurons. Immunohistochemistry experiments confirmed TRPV1 protein expression. In Ca2+ imaging experiments, capsaicin, a TRPV1 agonist, induced a significant increase in intracellular calcium ion concentration ([Ca2+](i)) in rat primary cultured VG neurons, which was almost completely blocked by capsazepine, a TRPV1-specific antagonist. Cinnamaldehyde, a TRPA1 agonist, also caused an increase in [Ca2+](i), which was completely inhibited by HC030031, a TRPA1-specific antagonist. Moreover, in some VG neurons, a [Ca2+](i); increase was evoked by both capsaicin and cinnamaldehyde in the same neuron. In summary, our histological and physiological studies reveal that TRPV1 and TRPA1 are expressed in VG neurons. It is suggested that TRPV1 and TRPA1 in VG neurons might participate in vestibular function and/or dysfunction such as vertigo. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2013.07.019

    Web of Science

    PubMed

    researchmap

  • [Endolymphatic hydrops detected with inner ear gd contrast-enhanced MRI; comparison between administration routes or with ECochG or glycerol test]. 査読

    Uno A, Horii A, Imai T, Osaki Y, Kamakura T, Kitahara T, Takimoto Y, Ohta Y, Morihana T, Nishiike S, Inohara H

    Nihon Jibiinkoka Gakkai kaiho   116 ( 8 )   960 - 8   2013年8月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3950/jibiinkoka.116.960

    PubMed

    researchmap

  • Pseudo-anterior canalolithiasis 査読

    Takao Imai, Chisako Masumura, Noriaki Takeda, Tadashi Kitahara, Atsuhiko Uno, Arata Horii, Suetaka Nishiike, Yumi Ohta, Kayoko Shingai-Higashi, Tetsuo Morihana, Suzuyo Okazaki, Takefumi Kamakura, Yasumitsu Takimoto, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   133 ( 6 )   594 - 599   2013年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INFORMA HEALTHCARE  

    Conclusion: Because nystagmus induced by ampullopetal inhibition of the posterior semicircular canal (PSCC) rotates around the axis perpendicular to the plane of the anterior semicircular canal (ASCC) of the other side, when free-floating debris is initially located at the distal portion of the PSCC, a patient showing positional nystagmus appears to have the ASCC type of benign paroxysmal positional nystagmus. We name this 'pseudo-anterior canalolithiasis'. Objective: We report on pseudo-anterior canalolithiasis originating in the PSCC and discuss the differential findings between pseudo-anterior and true anterior canalolithiasis by means of three-dimensional (3D) analysis of the positional nystagmus. Methods: We performed 3D analysis of the positional nystagmus in a patient with true anterior canalolithiasis and in another patient with pseudo-anterior canalolithiasis. Results: In the patient with true anterior canalolithiasis, the direction of positional nystagmus during reverse Epley maneuver was constant and its axis was perpendicular to the plane of the right ASCC three-dimensionally. In contrast, in the patient with pseudo-anterior canalolithiasis, the first positional nystagmus of which the axis was perpendicular to the plane of the left ASCC became a second positional nystagmus of which the axis was perpendicular to the plane of the right PSCC during the reverse Epley maneuver.

    DOI: 10.3109/00016489.2012.763180

    Web of Science

    PubMed

    researchmap

  • Psychological condition in patients with intractable Meniere's disease 査読

    Masashi Furukawa, Tadashi Kitahara, Arata Horii, Atsuhiko Uno, Takao Imai, Yumi Ohta, Tetsuo Morihana, Hidenori Inohara, Yasuo Mishiro, Masafumi Sakagami

    Acta Oto-Laryngologica   133 ( 6 )   584 - 589   2013年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Conclusions: Physicians should consider additional treatment strategies for Meniere's disease patients with a long history of disease and hearing loss in the secondary affected ear and also provide psychological support regarding future progressive bilateral hearing loss. Objectives: To treat intractable Meniere's disease patients effectively, we need to understand the psychological condition of each patient. We examined the state of neurosis and depression in patients and correlated this with demographic and background information. Methods: Between 1998 and 2009, we enrolled 207 patients with intractable Meniere's disease in this prospective study. We used the Cornell Medical Index and the Self-rating Depression Scale to evaluate their psychological condition. We also obtained demographic and background information relating to sex, age, duration of disease, vertigo frequency, hearing level in bilateral sides, and plasma vasopressin level. Results: Neurosis and depression was diagnosed in 40.1% and 60.4%, respectively, of patients with intractable Meniere's disease. Our results showed that surgical treatment significantly improved vertigo and hearing ability in patients with no psychological symptoms compared with those exhibiting psychological symptoms. Patients with a longer duration and worse hearing level in the secondary affected ear had a significantly higher incidence of mental illness than those with a shorter duration and better level of hearing. © 2013 Informa Healthcare.

    DOI: 10.3109/00016489.2012.759274

    Scopus

    PubMed

    researchmap

  • Effects of repeated optic flow stimulation on gait termination in humans 査読

    Suzuyo Okazaki, Suetaka Nishiike, Hiroshi Watanabe, Takao Imai, Atsuhiko Uno, Tadashi Kitahara, Arata Horii, Takefumi Kamakura, Yasumitsu Takimoto, Noriaki Takeda, Hidenori Inohara

    Acta Oto-Laryngologica   133 ( 3 )   246 - 252   2013年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Conclusions: Because the basic strategies to stop walking are stored as motor programs, visual stimulation may have little influence on body deviation during gait termination and its time course. Walking velocity, however, demonstrated dynamic flexible changes, which may subserve the stable process of gait termination under variable circumstantial changes such as optic flow. Objective: The aim of this study was to examine the effect of repeated optic flow on body deviation and walking velocity during gait termination, which may be more complicated than continuous standing or walking. Methods: Twenty-three healthy subjects were instructed to start walking upon an acoustic cue and to stop walking when the scenery changed in a virtual reality environment. Subjects underwent eight control trials without optic flow and three sets of optic flow conditions including four trials each of optic horizontal and rotational movement randomly. Results: Repeated optic flow caused no significant change of body deviation or the time course of the gait termination process in comparison with that in the control. The walking velocity at the start of the termination process showed short-term flexibility that denoted a gradual increase over the trial for within-set and long-term flexibility that denoted a gradual decrease for between-set. © 2013 Informa Healthcare.

    DOI: 10.3109/00016489.2012.740163

    Scopus

    PubMed

    researchmap

  • Psychological condition in patients with an acoustic tumor 査読

    Sachiko Hio, Tadashi Kitahara, Atsuhiko Uno, Takao Imai, Arata Horii, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   133 ( 1 )   42 - 46   2013年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INFORMA HEALTHCARE  

    Conclusions: This study showed that patients with acoustic tumor (AT) with hearing loss in the contralateral side had severe mood disturbances. It is important for physicians to provide patients who have better hearing in the tumor side with adequate information regarding the possibility of profound bilateral hearing loss and how to communicate with others in such situations. Objectives: We examined the pretreatment status as regards neurosis and/or depression of patients with an AT using the Cornell Medical Index (CMI) and Self-rating Depression Scale (SDS) and clarified the relationship with the patients' background. Methods: We enrolled 30 patients with unilateral AT between 1997 and 2010. At the time of diagnosis, we examined each patient's psychological condition, sex, age, laterality, tumor size, hearing level on bilateral side, facial paresis, headache, and canal paresis. Results: In all, 20.0% of AT patients were diagnosed with neurosis using the CMI and 26.7% had depression according to the SDS. chi(2) analysis showed that patients with AT with hearing deterioration on the contralateral side had mental illness significantly more often than those with normal hearing on the contralateral side. Multivariable regression analysis revealed that the pretreatment status of hearing level on the contralateral side significantly affected the patients' psychological condition.

    DOI: 10.3109/00016489.2012.709322

    Web of Science

    PubMed

    researchmap

  • The effect of visual-vestibulosomatosensory conflict induced by virtual reality on postural stability in humans 査読

    Suetaka Nishiike, Suzuyo Okazaki, Hiroshi Watanabe, Hironori Akizuki, Takao Imai, Atsuhiko Uno, Tadashi Kitahara, Arata Horii, Noriaki Takeda, Hidenori Inohara

    Journal of Medical Investigation   60 ( 3-4 )   236 - 239   2013年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we examined the effects of sensory inputs of visual-vestibulosomatosensory conflict induced by virtual reality (VR) on subjective dizziness, posture stability and visual dependency on postural control in humans. Eleven healthy young volunteers were immersed in two different VR conditions. In the control condition, subjects walked voluntarily with the background images of interactive computer graphics proportionally synchronized to their walking pace. In the visual-vestibulosomatosensory conflict condition, subjects kept still, but the background images that subjects experienced in the control condition were presented. The scores of both Graybiel's and Hamilton's criteria, postural instability and Romberg ratio were measured before and after the two conditions. After immersion in the conflict condition, both subjective dizziness and objective postural instability were significantly increased, and Romberg ratio, an index of the visual dependency on postural control, was slightly decreased. These findings suggest that sensory inputs of visual-vestibulosomatosensory conflict induced by VR induced motion sickness, resulting in subjective dizziness and postural instability. They also suggest that adaptation to the conflict condition decreases the contribution of visual inputs to postural control with re-weighing of vestibulosomatosensory inputs. VR may be used as a rehabilitation tool for dizzy patients by its ability to induce sensory re-weighing of postural control.

    DOI: 10.2152/jmi.60.236

    Scopus

    PubMed

    researchmap

  • Benign paroxysmal positional vertigo showing sequential translations of four types of nystagmus 査読

    Takao Imai, Noriaki Takeda, Atsuhiko Uno, Arata Horii, Tadashi Kitahara, Suetaka Nishiike, Kayoko Higashi-Shingai, Hidenori Inohara

    AURIS NASUS LARYNX   39 ( 5 )   544 - 548   2012年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Objective: We report a case of benign paroxysmal positional vertigo (BPPV) showing sequential translation of four types of nystagmus and discuss its pathophysiology.
    Methods: The case was 65-year-old female. We analyzed her nystagmus three-dimensionally. Results: At the first visit, she showed vertical-torsio nystagmus of the posterior canal type of BPPV (P-BPPV) and subsequently showed recently reported geotropic nystagmus with a long time constant. Two weeks later, she showed apogeotropic nystagmus of the horizontal canal type of BPPV (AH-BPPV) and subsequently a geotropic nystagmus with a short time constant of the horizontal canal type of BPPV (GH-BPPV).
    Conclusions: Three kind of nystagmus, namely P-BPPV, AH-BPPV and GH-BPPV can be explained by the otoconial debris hypothesis of the same ear. Finally, the recently reported geotropic nystagmus with a long time constant may be explained by a reversible lesion such as the denatured cupula or utricular imbalance of the same ear. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.anl.2011.10.007

    Web of Science

    PubMed

    researchmap

  • Transient low-tone air-bone gaps during convalescence immediately after canal plugging surgery for BPPV 査読

    Satoru Uetsuka, Tadashi Kitahara, Arata Horii, Takao Imai, Atsuhiko Uno, Suzuyo Okazaki, Takefumi Kamakura, Yasumitsu Takimoto, Hidenori Inohara

    AURIS NASUS LARYNX   39 ( 4 )   356 - 360   2012年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Objectives: The aim of the present study was to elucidate the time course and frequency patterns of transient low-tone air-bone gaps (ABGs) after canal plugging for intractable BPPV.
    Methods: We investigated eight patients with intractable BPPV who underwent canal plugging. Four were cases with posterior type (pBPPV) and the other four were those with horizontal type (hBPPV). Pure-tone audiometries (PTAs) were performed before and 7 days, 1 month and 6 months after surgery. ABGs (+) were defined as the three-tone-average >= 20 dB formulated by (a + b + c)/3, where a, b, and c are ABGs at 0.25, 0.5, and 1 kHz, respectively.
    Results: The ratio of the number of patients with ABGs (+) at the post-operative 7th day and 1st month was 100.0% (8/8). The ratio at the post-operative 6th month was 0.0% (0/8). There were no significant differences in the time course or frequency patterns of the ABGs between pBPPV and hBPPV.
    Conclusions: We clearly demonstrated eight cases with intractable BPPV showing transient low-tone ABGs during convalescence immediately after canal plugging. During that period, patients also complained of motion-evoked dizziness. All these findings suggest that, during such a convalescence period, the plugged area might not be fixed yet and could still induce the dizziness and low-tone ABGs, as enlarged vestibular aqueduct syndrome and superior semicircular canal deficiency syndrome exhibit low-tone ABGs due to the third mobile inner ear window. More than one month after surgery, both the ABGs and dizziness could disappear according to fixation of the plugged area. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.anl.2011.06.005

    Web of Science

    PubMed

    researchmap

  • 3D analysis of spontaneous upbeat nystagmus in a patient with astrocytoma in cerebellum 査読

    Kayoko Higashi-Shingai, Takao Imai, Noriaki Takeda, Atsuhiko Uno, Suetaka Nishiike, Arata Horii, Tadashi Kitahara, Yuka Fuse, Misako Hashimoto, Osamu Senba, Tsuyoshi Suzuki, Toshiaki Fujita, Hideo Otsuki, Hidenori Inohara

    AURIS NASUS LARYNX   39 ( 2 )   216 - 219   2012年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Aims: We report the case of a 58-year-old female patient who consulted our Department complaining of positional vertigo and showing spontaneous upbeat nystagmus (UBN) in darkness.
    Method: We analyzed her UBN three-dimensionally. The MRI scan revealed the astrocytoma in the left cerebellum involving the cerebellar vermis.
    Result: Three-dimensional analysis showed a spontaneous UBN rotating around the intra-aural axis in the pitch plane.
    Conclusion: Since the cerebellar vermis is known to plays an inhibitory role on the central vertical vestibule-ocular reflex (VOR), the present results suggest that the spontaneous UBN in darkness observed in this patient was induced by an imbalance of central vertical VOR tone. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.anl.2011.03.005

    Web of Science

    PubMed

    researchmap

  • Changes in beta-2 adrenergic receptor and AMP-activated protein kinase alpha-2 subunit in the rat vestibular nerve after labyrinthectomy 査読

    Tadashi Kitahara, Arata Horii, Atsuhiko Uno, Takao Imai, Suzuyo Okazaki, Takefumi Kamakura, Yasumitsu Takimoto, Hidenori Inohara

    NEUROSCIENCE RESEARCH   72 ( 3 )   221 - 226   2012年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    In the present study, to elucidate the role of vestibular ganglion (VG) after the unilateral labyrinthine damage, we examined quantitative changes in mRNA expression of beta-adrenergic receptors (bARs) and AMP-activated protein kinase alpha catalytic subunits (aAMPKs) in VG after unilateral labyrinthectomy (UL) in rats. Using the real-time PCR method, beta2 AR mRNA expression in bilateral VG and AMPK alpha2 mRNA expression in the ipsilateral VG were significantly up-regulated with the maximum increase at the postoperative 7 day and 1 day, respectively. The up-regulation of beta2 AR in bilateral VG was long-lasting until 28 days after UL and that of AMPK alpha2 in the ipsilateral VG was just transient within 7 days after UL These mRNA changes were supported by immunohistochemical data. According to previous reports, both of bARs and aAMPKs could regulate mitochondrial uncoupling protein (UCP) mRNA expression in several kinds of tissues and therefore might have thermogenic neurotransmission and antioxidant neuroprotective roles in neuronal tissues. UL requires not only long-lasting response of VG for central vestibular neuro-plasticity around 2-4 weeks but rapid response of VG against apoptosis of peripheral vestibular epithelia-neuronal synapses. The present findings suggest that beta2 AR in bilateral VG and AMPK alpha2 in the ipsilateral VG might play important signaling roles after the unilateral labyrinthine damage. (C) 2011 Elsevier Ireland Ltd and the japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/j.neures.2011.11.007

    Web of Science

    PubMed

    researchmap

  • Implication of substance P neuronal system in the amygdala as a possible mechanism for hypergravity-induced motion sickness 査読

    Arata Horii, Aya Nakagawa, Atsuhiko Uno, Tadashi Kitahara, Takao Imai, Suetaka Nishiike, Noriaki Takeda, Hidenori Inohara

    BRAIN RESEARCH   1435   91 - 98   2012年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We previously reported that motion sickness was prevented in rats with amygdala lesion and that provocative motion stimuli increased the number of Fos-positive neurons in the amygdala, suggesting that the amygdala is one of the neural substrates involved in the development of motion sickness. NK-1 receptors in the brain stem and amygdala are thought to play an important role in emesis and affective disorders, respectively. In the present study, to elucidate a role of substance P neuronal system and NK-1 receptors in the brain stem and amygdala in the development of motion sickness, we measured changes in gene expression of NK-1 receptors and preprotachykinin, a precursor of substance P, using quantitative real-time PCR methods in solitary tract nucleus and amygdala in rats after provocative motion stimuli induced by 2G hypergravity load. Effects of systemic administration of CP-99,994, an antagonist for NK-1 receptors, on hypergravity-induced motion sickness were also examined using pica behavior, eating non-nutritive substances such as kaolin, as an index of motion sickness in rats. Hypergravity-induced motion sickness was inhibited by CP-99,994 with a dose-dependent and enantioselective manner. Preprotachykinin mRNA expression was increased in basolateral nucleus of amygdala and solitary tract nucleus after hypergravity load for 3 h, whereas NK-1 receptor mRNA expression was not changed by hypergravity in amygdala and solitary tract nucleus. Present results suggest that 2G hypergravity load activated the substance P neuronal system in amygdala as well as in the brain stem and this activation would be related to the development of motion sickness. (C) 2011 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.brainres.2011.11.047

    Web of Science

    PubMed

    researchmap

  • Diagnosis of the subtype and affected ear of benign paroxysmal positional vertigo using a questionnaire 査読

    Kayoko Higashi-Shingai, Takao Imai, Tadashi Kitahara, Atsuhiko Uno, Yumi Ohta, Arata Horii, Suetaka Nishiike, Takayuki Kawashima, Taro Hasegawa, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   131 ( 12 )   1264 - 1269   2011年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INFORMA HEALTHCARE  

    Conclusion: The prediction of subtype and the affected ear of benign paroxysmal positional vertigo (BPPV) derived from the answers to our questionnaire can support the definitive diagnosis of BPPV. Objectives: We examined to what extent the diagnosis of subtype and the affected ear of BPPV judged from answers to a questionnaire agreed with the diagnosis decided by the results of the positional nystagmus test. Methods: We asked the following questions: 'What kind of head movements induce vertigo?' and 'How long does the vertigo continue?'. As for the affected ear, we asked which ear was lower during stronger vertigo when induced in a supine position or during sleep. Results: The percentages of correct diagnosis speculated by the combined answers were 69% in posterior canal-type BPPV, 48% in BPPV with geotropic nystagmus, and 39% in BPPV with apogeotropic nystagmus. The percentage of correct diagnoses of the affected ear was more than 80%.

    DOI: 10.3109/00016489.2011.611535

    Web of Science

    PubMed

    researchmap

  • Endolymphatic sac tumor with overexpression of V2 receptor mRNA and inner ear hydrops 査読

    Tadashi Kitahara, Chie Maekawa, Kaoru Kizawa, Takefumi Kamakura, Arata Horii, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   131 ( 9 )   951 - 957   2011年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INFORMA HEALTHCARE  

    Conclusion: We reported previously that hyperactivation of vasopressin type-2 receptor (V2R)-mediated signaling in the endolymphatic sac could affect endolymphatic fluid metabolism, resulting in the pathogenesis of endolymphatic hydrops. Taken together with the present endolymphatic sac tumor (ELST) study, it is suggested that disorder of V2R signaling in the endolymphatic sac for any reason could be involved in the pathogenesis of endolymphatic hydrops. Although it is due to tumor genesis in ELST, it is idiopathic in nature in Meniere's disease. Objective: We encountered two cases of ELST showing Meniere's disease-like symptoms. Both cases were suspected of having endolymphatic hydrops using neuro-otological examinations. To clarify the histopathological diagnosis of ELST and the molecular pathogenesis of endolymphatic hydrops, we performed histopathological and molecular biological examinations of the endolymphatic sac. Methods: ELSTs in two rare cases were removed completely through the transmastoidal approach. V2R mRNA expression was examined using real-time PCR. Results: The first case was diagnosed as inflammatory granulation adjacent to the endolymphatic sac, i.e. pseudo-ELST, and the second case was diagnosed as papillary adenoma of ELST. V2R mRNA expression was up-regulated in the endolymphatic sac of both cases as seen in Meniere's disease compared with controls.

    DOI: 10.3109/00016489.2011.580004

    Web of Science

    PubMed

    researchmap

  • Prognostic Factors of Peripheral Facial Palsy: Multivariate Analysis Followed by Receiver Operating Characteristic and Kaplan-Meier Analyses 査読

    Norihiko Takemoto, Arata Horii, Yoshiharu Sakata, Hidenori Inohara

    OTOLOGY & NEUROTOLOGY   32 ( 6 )   1031 - 1036   2011年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Objective: To search for prognostic predictors and reexamine the usefulness of electroneurography (ENoG) in predicting the prognosis of peripheral facial palsy using statistical methods.
    Study Design: Prospective study.
    Setting: Tertiary referral center.
    Patients: Consecutive 142 patients with Bell's palsy and 26 with Ramsay Hunt syndrome treated with steroid plus antiviral agents.
    Interventions: Multivariate analysis was used to identify which factors, including Yanagihara grading score and ENoG, predict better recovery. Receiver operating characteristic (ROC) curves were constructed for ENoG and grading score. The cumulative recovery rate by ENoG was calculated using Kaplan-Meier analysis. Recovery was defined as the improvement of grading score to 36 points or more (full score, 40) without synkinesis.
    Results: Multivariate analysis revealed that Ramsay Hunt syndrome, the worst grading score and ENoG were the significant prognostic predictors. The area under the ROC curve for ENoG was broader than those for grading score, indicating that ENoG was superior to grading score in terms of accuracy for prognosis prediction. The ROC curve revealed that more than 85% degeneration on ENoG had the best specificity (77.8%) and sensitivity (71.4%) to predict nonrecovery. When ENoG was subjected to the analysis of cumulative recovery rate using Kaplan-Meier plots, patients with more than 85% degeneration on ENoG had significantly poorer prognosis.
    Conclusion: ENoG was the most effective factor for prediction of the prognosis of peripheral facial palsy, and more than 85% degeneration had the best specificity and sensitivity to predict nonrecovery.

    DOI: 10.1097/MAO.0b013e31822558de

    Web of Science

    PubMed

    researchmap

  • Effects of cooling the pharyngeal mucosa after bipolar scissors tonsillectomy on postoperative pain 査読

    Arata Horii, Masayuki Hirose, Ryuichi Mochizuki, Keisuke Yamamoto, Masahiro Kawamoto, Tadashi Kitahara, Yoshifumi Yamamoto, Takayuki Kawashima, Atsuhiko Uno, Takao Imai, Suetaka Nishiike, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   131 ( 7 )   764 - 768   2011年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INFORMA HEALTHCARE  

    Conclusion: Bipolar scissors tonsillectomy followed by cooling down the pharyngeal mucosa has advantages in terms of postoperative pain and intraoperative blood loss compared with cold dissection. Objectives: The purpose of this study was to compare the postoperative pain between bipolar scissors tonsillectomy followed by cooling the pharyngeal mucosa and a traditional cold dissection. Methods: A total of 189 patients aged more than 16 years were operated due to habitual tonsillitis, obstructive sleep apnea syndrome, and IgA nephropathy. Of these, 79 patients were operated using bipolar scissors followed by cooling the pharyngeal mucosa with 4 degrees C saline for 10 min just after the removal of tonsils. The other 110 patients underwent cold dissection tonsillectomy. Outcome measures were intraoperative blood loss, operative time, postoperative pain evaluated on a visual analog scale, and postoperative secondary hemorrhage. Results: Significantly lower levels of intraoperative blood loss and less postoperative pain were obtained in bipolar scissors tonsillectomy followed by cooling the pharyngeal mucosa compared with cold dissection. Postoperative hemorrhage needing hemostatic surgery occurred in 2 of 79 patients who underwent bipolar scissors tonsillectomy with cooling, while it occurred in 1 of 110 patients after cold dissection. However, the difference was not statistically significant.

    DOI: 10.3109/00016489.2011.566580

    Web of Science

    PubMed

    researchmap

  • Endolymphatic hydrops in Meniere's disease detected by MRI after intratympanic administration of gadolinium: Comparison with sudden deafness 査読

    Arata Horii, Yasuhiro Osaki, Tadashi Kitahara, Takao Imai, Atsuhiko Uno, Suetaka Nishiike, Norihiko Fujita, Hidenori Inohara

    ACTA OTO-LARYNGOLOGICA   131 ( 6 )   602 - 609   2011年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INFORMA HEALTHCARE  

    Conclusion: The detection rate of endolymphatic hydrops was significantly higher in patients with Meniere's disease compared with those with sudden deafness, indicating that 3 T magnetic resonance imaging (MRI) with intratympanic gadolinium injection was effective in diagnosing endolymphatic hydrops. Objectives: To compare the detection rate of endolymphatic hydrops between patients with Meniere's disease and sudden deafness as controls by 3 T MRI after intratympanic gadolinium injection with conventional pulse sequence such as two-dimensional fluid-attenuated inversion recovery. Methods: Ten patients with unilateral Meniere's disease and eight with sudden deafness underwent inner ear MRI 24 h after intratympanic gadolinium injection. Results: The endolymphatic space was detected as a low signal intensity area, while the perilymphatic space showed high intensity by gadolinium enhancement. Due to faint enhancement, images could not be evaluated in 1 of 10 patients with Meniere's disease. However, the other nine patients together with two of the eight with sudden deafness were diagnosed as having hydrops. The difference in detection rates between the two diseases was statistically significant. Two hydrops-positive cases with sudden deafness were considered to be of the secondary type of hydrops, because images were taken after partial recovery from hearing loss several months after the onset of the disease.

    DOI: 10.3109/00016489.2010.548403

    Web of Science

    PubMed

    researchmap

  • Low-tone air-bone gaps after endolymphatic sac surgery 査読

    Tadashi Kitahara, Arata Horii, Yasuo Mishiro, Takayuki Kawashima, Takao Imai, Suetaka Nishiike, Hidenori Inohara

    AURIS NASUS LARYNX   38 ( 2 )   178 - 184   2011年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Objectives: We detected chronic low-tone air-bone gaps (LTABGs) in some patients with Meniere's disease after endolymphatic sac surgery. The aim of the present study was to elucidate the mechanism of LTABGs after endolymphatic sac surgery.
    Methods: We investigated 50 patients with Meniere's disease, who underwent surgery more than two years prior. LTABGs were defined as the three-tone-average = 20 dB formulated by (a + b + c)/3, where a, b, and c are ABGs at 0.25, 0.5, and 1 kHz, respectively (ABG +/-). The intra-operative finding was focused on identifying operculum (OPC +/-).
    Results: The ratio of post-operative ABG(+) was 50.0% (25/50). The ratio of intra-operative OPC(+) was 72.0% (36/50). The surgery results were as follows: the ratio of complete vertigo suppression (VS(+)) was 84.0% (42/50), air-conduction hearing gain (aHG(+)) was 40% (20/50), bone-conduction hearing gain (bHG(+)) was 64% (32/50), and speech discrimination gain (SDG(+)) was 28% (14/50). The postoperative ABG(+) was commonly observed in patients with intra-operative OPC(+) (chi-square test, p = 0.013). aHG(+) and SDG(+) results were related to the post-operative ABG(+) (chi-square test, p = 0.021 and p = 0.0018, respectively).
    Conclusions: These data suggest that intra-operative OPC(+) may be causative for post-operative ABG(+), resulting in post-operative aHG(+) and SDG(+). Thus, as enlarged vestibular aqueduct syndrome and superior semicircular canal deficiency syndrome exhibit LTABGs due to the third mobile inner ear window, endolymphatic sac surgery with adequate endolymphatic sac decompression and exposure to high doses of steroids, might induce LTABGs and the beneficial results of endolymphatic sac surgery. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.anl.2010.08.002

    Web of Science

    PubMed

    researchmap

  • Delayed Facial Nerve Palsy after Endolymphatic Sac Surgery

    Takefumi Kamakura, Tadashi Kitahara, Arata Horii, Hidenori Inohara

    JOURNAL OF INTERNATIONAL ADVANCED OTOLOGY   7 ( 1 )   130 - 133   2011年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MEDITERRANEAN SOC OTOLOGY & AUDIOLOGY  

    Objective: Delayed facial nerve palsy (DFP) after otological and neurotological surgeries is always observed ipsilateral to the operated side, while it is rare in other types of ENT surgery including head and neck, suggesting that DFP may result from procedures selective to temporal bone surgery. Herein, we present a rare case of DFP after endolymphatic sac surgery, and review the pathogenesis and prevention of DFP after otological and neurotological surgeries.
    Materials and Methods: The incidence of DFP after endolymphatic sac surgery from 1998 to 2008 at our hospital was 0.67% (1 out of 150 cases). A 44-year-old male with complaints of repeated vertigo attacks and cochlear symptoms such as persistent tinnitus and fluctuating hearing loss of the right ear. The patient received endolymphatic sac surgery on the right ear for treatment of intractable Meniere's disease, resulting in the onset of DFP.
    Results: DFP onset occurred at post-operative day 8, with the House-Brackmann grade III. HSV and VZV serum tests were negative.
    Conclusion: The later onset DFP observed in the present case after endolymphatic sac surgery might relate to the reactivation of a virus other than HSV and VZV in the geniculate ganglion induced by surgery.

    Web of Science

    researchmap

  • Secondary Endolymphatic Hydrops Following Sudden Deafness Detected by MRI after Intratympanic Administration of Gadolinium 査読

    Arata Horii, Junji Miyabe, Yasuhiro Osaki, Tadashi Kitahara, Takao Imai, Atsuhiko Uno, Suetaka Nishiike, Norihiko Fujita, Hidenori Inohara

    JOURNAL OF INTERNATIONAL ADVANCED OTOLOGY   7 ( 3 )   425 - 429   2011年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MEDITERRANEAN SOC OTOLOGY & AUDIOLOGY  

    Objective of this report is to alert the existence of secondary endolymphatic hydrops at the beginning of the era for diagnosing the hydrops by image analyses. A 64 years-old female who showed partial recovery of hearing disturbance two months after the onset of sudden deafness underwent inner ear MRI in combination with intratympanic injection of gadolinium. Two-dimensional fluid-attenuated inversion recovery (2D-FLAIR) sequences taken by 3 tesla MR unit showed endolymphatic hydrops in the cochlea. Electrocochleogram could not be performed due to high tone hearing loss. At present, inner ear MRI in combination with intratympanic injection of gadolinium has been mainly used for Meniere's patients to detect endolymphatic hydrops. However, this tool cannot distinguish the secondary hydrops as seen in our case from Meniere's disease and thus should be used with careful attention if used as a routine test for inner ear diseases.

    Web of Science

    researchmap

  • Microarray analysis of tonsils of IgA nephropathy patients.

    Iwatani H, Iio K, Nagasawa Y, Yamamoto R, Horii A, Okuzaki D, Inohara H, Nojima H, Imai E, Rakugi H, Isaka Y

    Advances in oto-rhino-laryngology   72   75 - 8   2011年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1159/000324611

    PubMed

    researchmap

  • A case of apogeotropic nystagmus with brainstem lesion: An implication for mechanism of central apogeotropic nystagmus 査読

    Takao Imai, Arata Horii, Noriaki Takeda, Tadashi Kitahara, Kayoko Higashi-Shingai, Hidenori Inohara

    AURIS NASUS LARYNX   37 ( 6 )   742 - 746   2010年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    We report a case showing apogeotropic nystagmus with the lesion of the brain stem, and discuss a possible mechanism of central apogeotropic nystagmus. The case was a 73-year-old male. We analyzed his nystagmus three-dimensionally. He showed apogeotropic nystagmus. Axis angles of slow phase eye velocity of his apogeotropic nystagmus were not in line with the axes perpendicular to the plane of horizontal semicircular canals, but with the patient's vertical axis. We then found that his nystagmus including the apogeotropic nystagmus was positioning, but not positional and that the direction of his positioning nystagmus was the same direction of postrotatory nystagmus after his head movement. His M RI scans showed an infarction around the prepositus hypoglossi nucleus of the brain. His apogeotropic nystagmus seemed to consist of a combination of prolonged postrotatory nystagmus after his head rotation to the left and right lateral position because the axis of postrotatory nystamus was in line with the axis of the head rotation. Therefore, it is suggested that a possible mechanism of central apogeotropic nystagmus is a prolonged postrotatory nystagmus after his head movement in the supine position due to the brain lesion involving the velocity storage mechanisms. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.anl.2010.04.004

    Web of Science

    PubMed

    researchmap

  • Microarray analysis of tonsils in immunoglobulin A nephropathy patients 査読

    Kenichiro Iio, Yasuyuki Nagasawa, Hirotsugu Iwatani, Ryohei Yamamoto, Arata Horii, Daisuke Okuzaki, Yoshiyuki Furumatsu, Hidenori Inohara, Hiroshi Nojima, Enyu Imai, Yoshitaka Isaka, Hiromi Rakugi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   393 ( 4 )   565 - 570   2010年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Background: Recently, combination of tonsillectomy and steroid pulse therapy was reported to be effective as the treatment of the immunoglobulin A nephropathy (IgAN). However, the gene expression difference between the tonsils in patients with IgAN and those in control patients is not established.
    Methods: We performed tonsillectomy combined with steroid pulse as a treatment to IgAN, analyzed the gene expression in the tonsils (N = 23) using microarray, compared with those with patients suffering from chronic tonsillitis (N = 22). From some candidate genes related with IgAN, we confirmed the apolipoprotein B messenger RNA-editing enzyme catalytic polypeptides 2 (APOBEC2) gene expression in the tonsil and we also analyzed its expression levels and clinical features.
    Results: Up-regulated genes seem to be categorized into two groups. One group belongs to the muscle related genes which might be caused by structural differences. The other group includes the immune system-related genes, such as APOBEC2, CALB2, DUSP27, and CXCL11. APOBEC2 was positively stained in the epithelium and the peripheral region of the germinal center in both tonsils. APOBEC2 expression level was negatively related with serum igg level, but did not correlate with clinical course after tonsillectomy.
    Conclusion: We confirmed gene expression differences related with immune system and muscle structure. The APOBEC2 was confirmed to be elevated in the tonsils with IgAN patients, and the gene expression level was negatively related with serum igg level in overall patients. These results might be helpful to reveal the mechanism of IgAN. (C) 2010 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2010.01.120

    Web of Science

    PubMed

    researchmap

  • Intractable Benign Paroxysmal Positioning Vertigo: Long-Term Follow-Up and Inner Ear Abnormality Detected by Three-Dimensional Magnetic Resonance Imaging 査読

    Arata Horii, Tadashi Kitahara, Yasuhiro Osaki, Takao Imai, Kazuyasu Fukuda, Masafumi Sakagami, Hidenori Inohara

    OTOLOGY & NEUROTOLOGY   31 ( 2 )   250 - 255   2010年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Objective: To investigate the occurrence rate, prognosis, and inner ear abnormality in intractable benign paroxysmal positioning vertigo (BPPV).
    Study Design: A prospective study.
    Setting: Tertiary referral university hospital.
    Patients: Intractable BPPV was defined in case of either a persistent nystagmus or a frequent relapse each lasting more than 1 year after the initial diagnosis.
    Intervention: T2-weighted 3-dimensional fast imaging employing steady-state acquisition sequences of magnetic resonance imaging (MRI) were reconstructed 3-dimensionally for 13 intractable BPPV patients and 14 control volunteers.
    Main Outcome Measure: Transition and relapse of nystagmus were monitored. Semicircular canals were evaluated for a stenosis or filling defect (obturation).
    Results: Eighteen patients (4 with posterior canal type, 2 with horizontal canal type with geotropic nystagmus, and 12 with apogeotropic nystagmus) fulfilled the above criteria for intractability among 495 BPPV patients. The occurrence rate of intractable BPPV was 3.6%. Also, the rate of nystagmus transition was significantly higher in patients with geotropic nystagmus and the posterior canal type (100%) compared with those with apogeotropic nystagmus (33.3%). Of the 13 intractable BPPV patients who underwent MRI, 11 (84.6%) had a total of 23 canals with abnormal appearance (29.5%), showing a significantly higher incidence compared with controls. There was no correlation between the affected canal diagnosed by MRI and the type of nystagmus.
    Conclusion: The low incidence of nystagmus transition in patients with apogeotropic nystagmus suggests a difference in pathophysiology between apogeotropic nystagmus and other types of BPPV. Stenosis and filling defect (obturation) of canals on MRI, which would indicate an innate narrowing and/or an otoconial jam of the semicircular canal, may account for the intractability of BPPV.

    DOI: 10.1097/MAO.0b013e3181cabd77

    Web of Science

    PubMed

    researchmap

  • Tinnitus as a prognostic factor of sudden deafness 査読

    Noriko Hikita-Watanabe, Tadashi Kitahara, Arata Horii, Takayuki Kawashima, Katsumi Doi, Shin-Ichi Okumura

    ACTA OTO-LARYNGOLOGICA   130 ( 1 )   79 - 83   2010年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS AS  

    Conclusions. The 'tinnitus-rare' group had a poorer prognosis for hearing than the 'tinnitus-often' group in all sudden sensorineural hearing loss (SSNHL), although the 'shorter duration' group had better prognosis than the 'longer duration' when restricted to SSNHL accompanied by tinnitus. This indicates that tinnitus itself may not be a sign for poor hearing prognosis but might be an essential sound for the initiation of repair of a damaged auditory system. Objectives. We examined the hearing improvement rate (HIR) and tinnitus at the onset of SSNHL to elucidate the prognostic value of tinnitus accompanying SSNHL. Patients and methods. Fifty patients with SSNHL were treated with systemic administration of steroids. Hearing recovery was determined by comparing the hearing levels before and after treatment. Tinnitus was subjectively evaluated by the tinnitus scoring questionnaire. The score for the five-step evaluation of the subjective tinnitus feelings 'loudness', 'duration' and 'annoyance' was obtained at the onset. Results. In terms of 'duration', when we divided all the cases into 'tinnitus-rare' group and 'tinnitus-often' group, HIR in the 'tinnitus-rare' group was significantly lower than that in 'tinnitus-often' group. When restricted to the 'tinnitus-often' group, HIR for 'shorter duration' was significantly higher than that for 'longer duration'.

    DOI: 10.3109/00016480902897715

    Web of Science

    PubMed

    researchmap

  • Plasma Vasopressin and V2 Receptor in the Endolymphatic Sac in Patients With Delayed Endolymphatic Hydrops 査読

    Tadashi Kitahara, Chie Maekawa, Kaoru Kizawa, Arata Horii, Katsumi Doi

    OTOLOGY & NEUROTOLOGY   30 ( 6 )   812 - 819   2009年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Objective: There are some kinds of sicknesses provoked by inadequate adaptation to physical and/or psychogenic stress in daily life. Delayed endolymphatic hydrops (DEH) is an inner ear disease like Meniere's disease (MD) characterized by episodic vertigo in the setting of preexisting unilateral deafness that especially occurs in civilized people with a stressful lifestyle. Its otopathologic finding was demonstrated to be inner ear endolymphatic hydrops through a temporal bone study in 1976, as in the case with MD in 1938. To elucidate the relationship between stress and the inner ear, we examined the plasma antidiuretic stress hormone vasopressin (pAVP) and its type 2 receptor (V2R) expression in the endolymphatic sac in patients with DEH.
    Study Design: A prospective molecular biological study.
    Methods: Between 1998 and 2007, we enrolled 20 patients with ipsilateral DEH to examine their pAVP during remission from vertigo attacks. Plasma vasopressin was also examined in 87 patients with unilateral MD and 30 control patients with chronic otitis media. Using the real-time polymerase chain reaction method with tissue samples obtained during surgery, we examined V2R mRNA expression in the endolymphatic sac in 6 patients with ipsilateral DEH, 9 patients with unilateral MD, and 6 control patients with acoustic neuroma.
    Results: Plasma vasopressin (1.5 times versus controls; unpaired t test, p = 0.140) and V2R mRNA expression in the endolymphatic sac (35.8 times versus controls; unpaired t test, p = 0.002) were higher in patients with DEH compared with those with acoustic neuroma. There were no significant differences in pAVP or V2R expression in the endolymphatic sac between DEH and MD. Patients with DEH showed a significantly negative correlation between pAVP and V2R (Pearson test, r = -0.92, p = 0.009) as in those with MD (Pearson test, r = -0.68, p = 0.043).
    Conclusion: Civilized people are frequently exposed to stress in their daily life, and pAVP can easily become elevated at any time. Therefore, a negative feedback system between pAVP and V2R in the endolymphatic sac may function for inner ear fluid homeostasis against stress-induced increases in pAVP. For the pathogenesis of endolymphatic hydrops resulting in vertigo attacks in patients with DEH as well as MD, pAVP may represent a matter of consequence, but V2R overexpression in the endolymphatic sac could be much more essential as a basis for these diseases.

    DOI: 10.1097/MAO.0b013e3181b11db5

    Web of Science

    PubMed

    researchmap

  • Two cases of spinal cord extramedullary tumor with positional vertiginous sensation. 査読

    Kitahara T, Kondoh K, Kizawa K, Horii A, Kubo T

    Acta oto-laryngologica. Supplementum   ( 562 )   50 - 2   2009年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/00016480902915673

    PubMed

    researchmap

  • Vestibular type of Mondini anomalies with BPPV and Meniere's disease-like symptoms 査読

    Chie Maekawa, Tadashi Kitahara, Arata Horii, Junji Miyabe, Takeshi Kubo

    AURIS NASUS LARYNX   36 ( 2 )   218 - 220   2009年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    An extremely rare case with labyrinthine anomaly with normal hearing was demonstrated. This case firstly showed transient positioning vertigo like benign paroxysmal positional vertigo (BPPV) and subsequently episodic vertigo like Meniere's disease. A 55-year-old mate attended our hospital, complaining of transient but persistent positioning vertigo in 2004. The apogeotropic positioning nystagmus was observed in spine position. In 2005, he came to feel episodic vertigo continuously for more than 30 min like Meniere's disease. We examined CT scan and 3D-MRI, indicating the hypoplastic lateral semicircular canal (L-SCC) fused together with enlarged vestibule on the left side. We finally diagnosed this case as Mondini anomaly on the left side.
    The imaging analysis is the most useful for differential diagnosis of Mondini anomalies, because symptoms in these patients are actually various due to the individual inner ear condition. The mechanism of generation of BPPV-Iike vertigo: the otolith in deformed utricle might be transferred into the fused space with vestibule and L-SCC, resulting in the irrigation of the hypoplastic cupula. The mechanism of generation of Meniere's disease-like vertigo: neuro-otologic findings with furosemide test implied endolymphatic hydrops on the left side. Meniere's disease-like episodic vertigo could be due to subsequently generated endolymphatic hydrops. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.anl.2008.04.010

    Web of Science

    PubMed

    researchmap

  • Two cases of spinal cord extramedullary tumor with positional vertiginous sensation

    Tadashi Kitahara, Kazumasa Kondoh, Kaoru Kizawa, Arata Horii, Takeshi Kubo

    ACTA OTO-LARYNGOLOGICA   129   50 - 52   2009年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS AS  

    Conclusions. We conclude that neck imaging should be carried out for patients with persistent paroxysmal positional vertigo following diagnostic and/or therapeutic maneuvers. Objective. It is sometimes complicated to diagnose patients with vertigo that is transiently induced by head and neck positioning. Neck-vestibular diseases also induce vertiginous sensation with head and neck movement and need to be ruled out for the diagnosis of benign paroxysmal positional vertigo (BPPV). Patients and methods. Two elderly female patients visited our hospital with complaints of transient vertigo induced by Dix-Hallpike positioning, suggesting posterior canal BPPV. We carried out gadolinium-enhanced neck MRI in both these cases. Results. The positional nystagmus was not clearly observed or vertiginous sensation did not show any decay during repeated vestibular examination in either case. These cases were finally diagnosed as spinal cord intradural extramedullary tumor (C3-C4) by means of neck MRI.

    DOI: 10.1080/00016480902915673

    Web of Science

    researchmap

  • Effects of hypergravity on histamine H1 receptor mRNA expression in hypothalamus and brainstem of rats: implications for development of motion sickness 査読

    Go Sato, Atsuhiko Uno, Arata Horii, Hayato Umehara, Yoshiaki Kitamura, Kazunori Sekine, Koichi Tamura, Hiroyuki Fukui, Noriaki Takeda

    ACTA OTO-LARYNGOLOGICA   129 ( 1 )   45 - 51   2009年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS AS  

    Conclusion: The study findings suggest that histamine was released from the axon terminals in the hypothalamus and brainstem and the released histamine activated post-synaptic H1 receptors there, resulting in the development of motion sickness. Objectives: We first examined which subtype of post-synaptic histaminergic receptor was responsible for the development of motion sickness. We then examined whether H1 receptors were up-regulated in various areas of the rat brain after 2 G hypergravity load, because the stimulation of H1 receptor was reported to up-regulate the level of H1 receptor protein expression through augmentation of H1 receptor mRNA expression. Materials and methods: For this purpose, we used an animal model of motion sickness, using pica (eating non-nutritive substances such as kaolin), as a behavioral index in rats. Results: After 2 G hypergravity load, rats ate a significant amount of kaolin, indicating that they suffered from motion sickness. The hypergravity-induced kaolin intake was suppressed by mepyramine, but not by terfinadine or zolantizine. This finding indicates that cerebral post-synaptic H1 but not H2 or peripheral H1 receptors play an important role in the development of motion sickness. The expression of H1 receptor mRNA was up-regulated in the hypothalamus and brainstem, but not in the cerebral cortex after 2 G hypergravity load in rats.

    DOI: 10.1080/00016480802008173

    Web of Science

    PubMed

    researchmap

  • Changes in mitochondrial uncoupling protein expression in the rat vestibular nerve after labyrinthectomy 査読

    Tadashi Kitahara, Arata Horii, Kaoru Kizawa, Chie Maekawa, Takeshi Kubo

    NEUROSCIENCE RESEARCH   59 ( 3 )   237 - 242   2007年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    In the present study, to elucidate the role of mitochondrial uncoupling proteins (UCPs) in inner ear, we examined quantitative changes in the mRNA expression in vestibular ganglion (VG) after unilateral labyrinthectomy (UL) in rats. Using real-time PCR methods, UCP2, 3 and 4 mRNA expressions in the ipsilateral VG were significantly up-regulated with the maximum increase at the post-operative 1 day and all but UCP2 returned to the control level 1 week after UL. UCP2 mRNA expression was significantly up-regulated even 4 weeks after UL. Only UCP2 mRNA expression in the contralateral VG was gradually up-regulated between 1 and 4 weeks after UL. According to previous reports, UCP2 and 3 as well as UCP1 were thermogenic in yeast and brain UCP2 was suggested to modulate pre- and post-synaptic events by axonal thermogenesis. It was also reported that UCP1, 2 and 3 responses to superoxide application were an antioxidant protective mechanism. These findings suggest that mitochondrial UCPs could play both a neuro-protective role against oxidative damage and a thermal signaling role for neuro-modulation in vestibular nerve. (C) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/j.neures.2007.07.001

    Web of Science

    PubMed

    researchmap

  • [Dynamic vestibular compensation in vestibular peripheral diseases]. 査読

    Kitahara T, Horii A, Kondoh K, Okumura S, Kubo T

    Nihon Jibiinkoka Gakkai kaiho   110 ( 11 )   720 - 7   2007年11月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3950/jibiinkoka.110.720

    PubMed

    researchmap

  • Unilateral vestibular deafferentation-induced changes in calcium signaling-related molecules in the rat vestibular nuclear complex 査読

    Chisako Masumura, Arata Horii, Kenji Mitani, Tadashi Kitahara, Atsuhiko Uno, Takeshi Kubo

    BRAIN RESEARCH   1138   129 - 135   2007年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Inquiries into the neurochemical mechanisms of vestibular compensation, a model of lesion-induced neuronal plasticity, reveal the involvement of both voltage-gated Ca2+ channels (VGCC) and intracellular Ca2+ signaling. Indeed, our previous microarray analysis showed an up-regulation of some calcium signaling-related genes such as the alpha 2 subunit of L-type calcium channels, calcineurin, and plasma membrane Ca2+ ATPase 1 (PMCA1) in the ipsilateral vestibular nuclear complex (VNC) following unilateral vestibular deafferentation (UVD). To further elucidate the role of calcium signaling-related molecules in vestibular compensation, we used a quantitative real-time polymerase chain reaction (PCR) method to confirm the microarray results and investigated changes in expression of these molecules at various stages of compensation (6 h to 2 weeks after UVD). We also investigated the changes in gene expression during Bechterew's phenomenon and the effects of a calcineurin inhibitor on vestibular compensation. Real-time PCR showed that genes for the alpha 2 subunit of VGCC, PMCA2, and calcineurin were transiently up-regulated 6 h after UVD in ipsilateral VNC. A subsequent UVD, which induced Bechterew's phenomenon, reproduced a complete mirror image of the changes in gene expressions of PMCA2 and calcineurin seen in the initial UVD, while the a2 subunit of VGCC gene had a trend to increase in VNC ipsilateral to the second lesion. Pre-treatment by FK506, a calcineurin inhibitor, decelerated the vestibular compensation in a dose-dependent manner. Although it is still uncertain whether these changes in gene expression are causally related to the molecular mechanisms of vestibular compensation, this observation suggests that after increasing the Ca2+ influx into the ipsilateral VNC neurons via up-regulated VGCC, calcineurin may be involved in their synaptic plasticity. Conversely, an up-regulation of PMCA2, a brain-specific Ca2+ pump, would increase an efflux of Ca2+ from those neurons and perhaps prevent cell damage following UVD. (c) 2007 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.brainres.2006.12.072

    Web of Science

    PubMed

    researchmap

  • Effects of fluvoxamine on anxiety, depression, and subjective handicaps of chronic dizziness patients with or without neuro-otologic diseases 査読

    Arata Horii, Atsuhiko Uno, Tadashi Kitahara, Kenji Mitani, Chisako Masumura, Kaoru Kizawa, Takeshi Kubo

    JOURNAL OF VESTIBULAR RESEARCH-EQUILIBRIUM & ORIENTATION   17 ( 1 )   1 - 8   2007年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IOS PRESS  

    A prospective, open-label clinical trial was conducted for two aims: first, to evaluate the role of fluvoxamine, one of selective serotonin reuptake inhibitors, in the treatment of dizziness for the first time and to investigate its effective mechanisms. Second, to test the hypothesis that dizziness in patients without abnormal neuro-otologic findings would be induced by psychiatric disorders rather than by unnoticed neuro-otologic diseases. Nineteen patients with neuro-otologic diseases (Group I) and 22 patients in whom standard vestibular tests revealed no abnormal findings (Group II) were treated by fluvoxamine (200 mg/day) for eight weeks. Subjective handicaps due to dizziness using a questionnaire, anxiety and depressive symptoms measured with the Hospital Anxiety and Depression Scale (HADS), and stress hormones (vasopressin and cortisol) were examined before and 8 weeks after treatment. Overall, fluvoxamine decreased subjective handicaps of both Groups I and II. Fluvoxamine decreased HADS of only patients whose subjective handicaps were reduced (= responders) in both groups, suggesting that fluvoxamine was effective for dizziness via psychiatric action rather than a recovery of vestibular function through serotonergic activation. In non-responders of Group II, pre-treatment HADS was higher than in Group I non-responders and it was not decreased by the treatment, suggesting that dizziness of Group II non-responders was due to severe psychiatric disorders rather than unnoticed neuro-otologic diseases. Anxiety and depression components of HADS showed a good correlation at both pre- and post-treatment periods. No post-therapeutic decrease was observed in either vasopressin or cortisol even in responders, suggesting that dizziness was not the sole cause of stress in chronic dizziness patients. In conclusion, patients with or without physical neuro-otologic deficits who report chronic dizziness accompanied by anxiety and depression (as measured by HADS) showed improvements across a full range of subjective handicaps and psychological distress, while patients with physical neuro-otologic defects and minimal anxiety or depression did not benefit. The main causes of dizziness in patients without physical neuro-otologic findings were psychiatric disorders.

    Web of Science

    PubMed

    researchmap

  • Factors relating to the vertigo control and hearing changes following intratympanic gentamicin for intractable Ménière's disease. 査読

    Horii A, Saika T, Uno A, Nishiike S, Mitani K, Nishimura M, Kitahara T, Fukushima M, Nakagawa A, Masumura C, Sasaki T, Kizawa K, Kubo T

    Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology   27 ( 6 )   896 - 900   2006年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1097/01.mao.0000227663.79210.c1

    PubMed

    researchmap

  • [Delayed facial nerve palsy after otologic surgery]. 査読

    Kitahara T, Kubo T, Doi K, Mishiro Y, Kondoh K, Horii A, Okumura S, Miyahara H

    Nihon Jibiinkoka Gakkai kaiho   109 ( 7 )   600 - 5   2006年7月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3950/jibiinkoka.109.600

    PubMed

    researchmap

  • Lesions of the vestibular system disrupt hippocampal theta rhythm in the rat 査読

    NA Russell, A Horii, PF Smith, CL Darlington, DK Bilkey

    JOURNAL OF NEUROPHYSIOLOGY   96 ( 1 )   4 - 14   2006年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    The hippocampus has a major role in memory for spatial location. Theta is a rhythmic hippocampal EEG oscillation that occurs at similar to 8 Hz during voluntary movement and that may have some role in encoding spatial information. We investigated whether, as part of this process, theta might be influenced by self-movement signals provided by the vestibular system. The effects of bilateral peripheral vestibular lesions, made >= 60 days prior to recording, were assessed in freely moving rats. Power spectral analysis revealed that theta in the lesioned animals had a lower power and frequency compared with that recorded in the control animals. When the electroencephalography (EEG) was compared in epochs matched for speed of movement and acceleration, theta was less rhythmic in the lesioned group, indicating that the effect was not a result of between-group differences in this behavior. Blood measurements of corticosterone were also similar in the two groups indicating that the results could not be attributed to changes in stress levels. Despite the changes in theta EEG, individual neurons in the CA1 region of lesioned animals continued to fire with a periodicity of similar to 8 Hz. The positive correlation between cell firing rate and movement velocity that is observed in CA1 neurons of normal animals was also maintained in cells recorded from lesion group animals. These findings indicate that although vestibular signals may contribute to theta rhythm generation, velocity-related firing in hippocampal neurons is dependent on nonvestibular signals such as sensory flow, proprioception, or motor efference copy.

    DOI: 10.1152/jn.00953.2005

    Web of Science

    PubMed

    researchmap

  • Fos-enkephalin signaling in the rat medial vestibular nucleus facilitates vestibular compensation 査読

    T Kitahara, T Kaneko, A Horii, M Fukushima, K Kizawa-Okumura, N Takeda, T Kubo

    JOURNAL OF NEUROSCIENCE RESEARCH   83 ( 8 )   1573 - 1583   2006年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    In the present study, we first observed up-regulation in preproenkephalin (PPE)-like immunoreactivity (-LIR), a precursor of Met- and Leu-enkephalin, in the rat ipsilateral medial vestibular nucleus (ipsi-MVN) after unilateral labyrinthectomy (UL). By means of double-staining immunohistochemistry with PPE and Fos, a putative regulator of PPE gene expression, we revealed that some of these PPE-LIR neurons were also Fos immunopositive. The time course of decay of these double-stained neurons was quite parallel to that of UL-induced behavioral deficits. This suggests that these double-labeled neurons could have something to do with development of vestibular compensation. We next examined correlation between Fos and PPE expression in the ipsi-MVN by means of a 15-min pre-UL application of antisense oligonucleotide probes against c-fos mRNA into the ipsi-MVN. Gel shift assay and Western blotting revealed that elimination of Fos expression significantly reduced both AP-1 DNA binding activity and PPE expression in the ipsi-MVN after UL. C-fos antisense study also revealed that depression of Fos-PPE signaling in the ipsi-MVN caused significantly more severe behavioral deficits during vestibular compensation. Furthermore, studies with PPE antisense and naloxone, an opioid receptor antagonist, demonstrated that specific depression of enkephalinergic effects in the ipsi-MVN significantly delayed vestibular compensation. All these findings suggest that, immediately after UL, Fos induced in some of the ipsi-MVN neurons could regulate consequent PPE expression via the AP-1 activation and facilitate the restoration of balance between bilateral MVN activities via the opioid receptor activation, resulting in progress of vestibular compensation. (c) 2006 Wiley-Liss, Inc.

    DOI: 10.1002/jnr.20830

    Web of Science

    PubMed

    researchmap

  • Endolymphatic hydrops as a cause of audio-vestibular manifestations in relapsing polychondritis 査読

    J Murata, A Horii, M Tamura, K Mitani, M Mizuki, T Kubo

    ACTA OTO-LARYNGOLOGICA   126 ( 5 )   548 - 552   2006年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS AS  

    Relapsing polychondritis ( RP) is characterized by inflammation and subsequent degeneration of cartilage. We report a 61-year-old woman who had RP with audio-vestibular manifestations. She was also diagnosed as having a myelofibrosis with myeloid metaplasia (MMM). Bilateral endolymphatic hydrops ( EH) was confirmed by dominant - SP/AP of the electrocochleogram (ECochG). When thalidomide and prednisolone were prescribed for the treatment of MMM, symptoms of RP - including the inner ear dysfunction - were ameliorated. Isosorbide, one of the osmotic diuretics commonly used for the treatment of Meniere's disease ( MD) in Japan, was also effective in keeping her free from inner ear dysfunction. This is the first report to confirm the existence of EH in a patient with RP with audio-vestibular manifestations. We suppose that an immunological imbalance due to MMM, in conjunction with a specific immunogenetic background, may have played a role in the pathogenesis of RP and the formation of EH in this patient.

    DOI: 10.1080/00016480500437369

    Web of Science

    PubMed

    researchmap

  • Magnetic resonance imaging of syringocystadenoma papilliferum of the external auditory canal 査読

    T Kamakura, A Horii, Y Mishiro, S Takashima, T Kubo

    AURIS NASUS LARYNX   33 ( 1 )   53 - 56   2006年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Syringocystadenoma papilliferum (SCAP) usually occurs on the face or the scalp and is very rare in the external auditory canal (EAC). There has been no information on magnetic resonance (MR) imaging of this tumor irrespective of its site. We report here a case of 57-year-old man having this tumor. which was surgically removed and its histopathology was confirmed. MR imaging demonstrated a lobulated 4-cm mass with clearly defined margins in the EAC. Although the tumor was bulky, these MR findings were different from the malignancies. The mass lesion showed intermediate signal intensity both on T1- and T2-weighted MR images and showed slight enhancement on gadolinium-enhanced T1-weighted images. signal intensities oil T2-weighted images of this tumor were low compared to those of pleomorphic adenoma. All ceruminous gland tumors including SCAP are thought to be potentially malignant therefore, pre-operative biopsy should not be performed. Even though incisional biopsy is sometimes needed as in our case, the current MR features would be helpful for differential diagnosis of this rare condition and assessing the extension of the tumor. (C) 2005 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.anl.2005.07.011

    Web of Science

    PubMed

    researchmap

  • Intracranial vertebral artery dissection mimicking acute peripheral vertigo 査読

    A Horii, K Okumura, T Kitahara, T Kubo

    ACTA OTO-LARYNGOLOGICA   126 ( 2 )   170 - 173   2006年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS AS  

    When diagnosing and treating patients with acute vertigo, the clinician must differentiate brain lesions from benign peripheral disorders. We here report a rare case of acute vertigo caused by intracranial vertebral artery dissection mimicking peripheral disease. A 67-year-old man presented with spontaneous nystagmus and moderate ataxia preceded by neck pain. No other neurological signs were observed, suggesting acute peripheral vertigo. However, magnetic resonance imaging (MRI) demonstrated a cerebellar infarction. Simultaneous magnetic resonance angiography (MRA) showed no flow void of the left vertebral artery and contrast-enhanced MRA demonstrated a double lumen sign, suggesting that vertebral artery dissection was a cause of infarction. The clinical course was favorable without anticoagulation drugs, which are sometimes contraindicated because of the potential risk of subarachnoid hemorrhage. Vertebral artery dissection can cause cerebellar infarction in patients without vascular risk factors mimicking acute peripheral vertigo. Careful history regarding the neck pain is important and MRA in combination with MRI can replace angiography in diagnosing this disorder.

    DOI: 10.1080/00016480500279977

    Web of Science

    PubMed

    researchmap

  • The effects of vestibular lesions on hippocampal function in rats 査読

    PF Smith, A Horii, N Russell, DK Bilkey, YW Zheng, P Liu, DS Kerr, CL Darlington

    PROGRESS IN NEUROBIOLOGY   75 ( 6 )   391 - 405   2005年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Interest in interaction between the vestibular system and the hippocampus was stimulated by evidence that peripheral vestibular lesions could impair performance in learning and memory tasks requiring spatial information processing. By the 1990s, electrophysiological data were emerging that the brainstem vestibular nucleus complex (VNC) and the hippocampus were connected polysynaptically and that hippocampal place cells could respond to vestibular stimulation. The aim of this review is to summarise and critically evaluate research published in the last 5 years that has seen major progress in understanding the effects of vestibular damage on the hippocampus. In addition to new behavioural studies demonstrating that animals with vestibular lesions exhibit impairments in spatial memory tasks, electrophysiological studies have confirmed long-latency, polysynaptic pathways between the VNC and the hippocampus. Peripheral vestibular lesions have been shown to cause long-term changes in place cell function, hippocampal EEG activity and even CA1 field potentials in brain slices maintained in vitro. During the same period, neurochemical investigations have shown that some hippocampal subregions exhibit long-term changes in the expression of neuronal nitric oxide synthase, arginase I and II, and the NR1 and NR2A N-methyl-D-aspartate (NMDA) receptor subunits following peripheral vestibular damage. Despite the progress, a number of important issues remain to be resolved, such as the possible contribution of auditory damage associated with vestibular lesions, to the hippocampal effects observed. Furthermore, although these studies demonstrate that damage to the vestibular system does have a long-term impact on the electrophysiological and neurochemical function of the hippocampus, they do not indicate precisely how vestibular information might be used in hippocampal functions such as developing spatial representations of the environment. Understanding this will require detailed electrical stimulation and lesion studies to elucidate the way in which different kinds of vestibular information are transmitted to various hippocampal subregions. (c) 2005 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.pneurobio.2005.04.004

    Web of Science

    PubMed

    researchmap

  • Immunocytochemical and stereological analysis of GABA(B) receptor subunit expression in the rat vestibular nucleus following unilateral vestibular deafferentation 査読

    R Zhang, J Ashton, A Horii, CL Darlington, PF Smith

    BRAIN RESEARCH   1037 ( 1-2 )   107 - 113   2005年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The process of behavioural recovery that occurs following damage to one vestibular labyrinth, vestibular compensation, has been attributed in part to a down-regulation of GABA(B) receptors in the vestibular nucleus complex (VNC) ipsilateral to the lesion, which could potentially reduce commissural inhibition from the contralateral VNC. In this study, we tested the possibility that this occurs through a decrease in the expression of either the GABA(B1) or GABA(B2) subunits of the GABA(B) receptor. We used Western blotting to quantify the expression of these subunits in the VNC at 10 h and 50 h following unilateral vestibular deafferentation (UVD) or sham surgery in rats. We then used immunocytochemistry and stereological counting methods to estimate the number of neurons expressing these subunits in the MVN at 10 h and 2 weeks following UVD or sham surgery. Compared to sham controls, we found no significant changes in either the expression of the two GABA(B) receptor subunits in the VNC or in the number of MVN neurons expressing these GABA(B) receptor subunits post-UVD. These results suggest that GABA(B) receptor expression does not change substantially in the VNC during the process of vestibular compensation. (c) 2005 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.brainres.2005.01.018

    Web of Science

    PubMed

    researchmap

  • Does vestibular damage cause cognitive dysfunction in humans? 査読

    PF Smith, YW Zheng, A Horii, CL Darlington

    JOURNAL OF VESTIBULAR RESEARCH-EQUILIBRIUM & ORIENTATION   15 ( 1 )   1 - 9   2005年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IOS PRESS  

    For more than a decade, evidence from animal studies has suggested that damage to the vestibular system leads to deficits in spatial navigation which are indicative of impaired spatial learning and memory. More recently, direct evidence has emerged to demonstrate that humans with vestibular disorders exhibit a range of cognitive deficits that are not just spatial in nature, but also include non-spatial functions such as object recognition memory. Vestibular dysfunction has been shown to adversely affect attentional processes and increased attentional demands can worsen the postural sway associated with vestibular disorders. Recent MRI studies also show that humans with bilateral vestibular damage undergo atrophy of the hippocampus which correlates with their degree of impairment on spatial memory tasks. These results are consistent with those from animal studies and, together, suggest that humans with vestibular disorders are likely to experience cognitive dysfunction which is not necessarily related to any particular episode of vertigo or dizziness, and therefore may occur even in patients who are otherwise well compensated. These findings may be related to the observation that patients with vestibular deficits experience a high incidence of depression and anxiety disorders.

    Web of Science

    PubMed

    researchmap

  • Ménière's disease is associated with single nucleotide polymorphisms in the human potassium channel genes, KCNE1 and KCNE3. 査読

    Doi K, Sato T, Kuramasu T, Hibino H, Kitahara T, Horii A, Matsushiro N, Fuse Y, Kubo T

    ORL; journal for oto-rhino-laryngology and its related specialties   67 ( 5 )   289 - 93   2005年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1159/000089410

    PubMed

    researchmap

  • Impaired spatial learning after hypergravity exposure in rats 査読

    K Mitani, A Horii, T Kubo

    COGNITIVE BRAIN RESEARCH   22 ( 1 )   94 - 100   2004年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Most astronauts experience spatial disorientation after exposure to weightlessness, indicating that constant gravity is utilized as a stable external reference during spatial cognition. We attempted to elucidate the role of constant gravity in spatial learning using a radial arm maze test on rats housed in a hypergravity environment (HG) produced by a centrifuge device. Male Wistar rats were kept in 2G linear acceleration for 2 weeks before the spatial learning task, which lasted for 10 days. The control rats were placed close to the centrifuge device but not exposed to hypergravity. Spatial learning was evaluated by the accuracy and the re-entry rate, which were the rate of correct arm entries and the rate of entries into the arms that they had already visited, respectively. Locomotor activity was measured by number of entries per minute. The number of baits the animal took per minute was also measured. The results showed that accuracy was significantly inferior and the re-entry rate was significantly higher in the HG rats than in the controls, suggesting that animals use a constant gravity as a stable external reference in spatial learning. However, these differences disappeared at 5 days later, indicating that the HG rats learned the spatial task more rapidly than the controls. Locomotor activity was higher in the HG rats and there was no difference in number of baits per minute between the HG and control animals. In conclusion, if one sensory cue necessary for spatial cognition is disturbed by gravity change, animals can subsidize with other sensory Cues Such as proprioceptive and motor efference copy signals through increased locomotor activities. (C) 2004 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.cogbrainres.2004.08.002

    Web of Science

    PubMed

    researchmap

  • Microarray analysis of gene expression in the rat vestibular nucleus complex following unilateral vestibular deafferentation 査読

    A Horii, C Masumura, PF Smith, CL Darlington, T Kitahara, A Uno, K Mitani, T Kubo

    JOURNAL OF NEUROCHEMISTRY   91 ( 4 )   975 - 982   2004年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING LTD  

    To investigate the molecular background of vestibular compensation, a model of lesion-induced plasticity, we used a microarray analysis to examine genes that show asymmetrical expression between the bilateral vestibular nucleus complexes (VNCs) 6 h following unilateral vestibular deafferentation (UVD). Asymmetrical gene expression was then validated by a real-time quantitative PCR. Among the 88 genes for which the ipsilateral (ipsi) : contralateral (contra) was > 1.35, the number of known genes was 33 (38%), and the number of expressed sequence tag (EST) sequences was 55 (62%). Among the 130 genes for which the contra : ipsi was > 1.35, the number of known genes was 55 (42%), and the number of EST sequences was 75 (58%). Changes in some of the genes were consistent with previous studies; however, we found several new genes which could be functionally related to the molecular basis of the electrophysiological asymmetry between the VNCs following UVD. Ipsi > contra genes included the GABA(A) receptor rho subunit, regulatory proteins of G protein signaling, calcium signaling related molecules such as the voltage-dependent calcium channel alpha2/delta subunit 1, calcineurin subunit Abeta and Ca2+ pump. Contra > ipsi genes included the neuronal high affinity glutamate transporter, 5-hydroxytryptamine receptor 1D, mitogen-activated protein kinase 12 and ubiquitin carboxy-terminal hydrolase L1.

    DOI: 10.1111/j.1471-4159.2004.02781.x

    Web of Science

    PubMed

    researchmap

  • Paroxetine, a selective serotonin reuptake inhibitor, reduces depressive symptoms and subjective handicaps in patients with dizziness. 査読

    Horii A, Mitani K, Kitahara T, Uno A, Takeda N, Kubo T

    Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology   25 ( 4 )   536 - 43   2004年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1097/00129492-200407000-00022

    PubMed

    researchmap

  • Vestibular influences on CA1 neurons in the rat hippocampus: an electrophysiological study in vivo 査読

    A Horii, NA Russell, PF Smith, CL Darlington, DK Bilkey

    EXPERIMENTAL BRAIN RESEARCH   155 ( 2 )   245 - 250   2004年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG  

    Vestibular information is known to be important for accurate spatial orientation and navigation. Hippocampal place cells, which appear to encode an animal's location within the environment, are also thought to play an essential role in spatial orientation. Therefore, it can be hypothesized that vestibular information may influence cornu ammonis region 1 (CA1) hippocampal neuronal activity. To explore this possibility, the effects of electrical stimulation of the medial vestibular nucleus (MVN) on the firing rates of hippocampal CA1 neurons in the urethane-anesthetized rat were investigated using extracellular single unit recordings. The firing rates of CA1 complex spike cells (n=29), which most likely correspond to place cells, consistently increased during electrical stimulation of the MVN in a current intensity dependent manner. Stimulation applied adjacent to the MVN failed to elicit a response. Overall, the firing rates of non-complex spike cells (n=22) did not show a consistent response to vestibular stimulation, although in some cells clear responses to the stimulation were observed. These findings suggest that vestibular inputs may contribute to spatial information processing in the hippocampus.

    DOI: 10.1007/s00221-003-1725-9

    Web of Science

    PubMed

    researchmap

  • Expression of Musashi 1, a neural RNA-binding protein, in the cochlea of young adult mice 査読

    J Murata, A Murayama, A Horii, K Doi, T Harada, H Okano, T Kubo

    NEUROSCIENCE LETTERS   354 ( 3 )   201 - 204   2004年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    Musashi1 (Msi1) is an RNA-binding protein expressed in neural stem/progenitor cells, astroglial progenitor cells and astrocytes in the vertebrate central nervous system. We hypothesized that Msi I is expressed in only some of the supporting cells in the cochlea, which could become hair cell progenitors under special circumstances after an injury. To observe this, we investigated Msi1 expression in young adult mouse cochlea by immunohistochemistry using monoclonal antibody against Msi1. Msi1 immunostaining was found in a variety of supporting cells but not in outer hair cells in the organ of Corti. Although an immunoreactive ring was found around the inner hair cells, it also seemed to originate from the supporting cells. We suppose that this wide expression of Msi1 in supporting cells indicates that those cells might have the potential to become hair cell progenitors if injured, but that some other mechanisms strictly inhibit this ability. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2003.10.036

    Web of Science

    PubMed

    researchmap

  • Effects of unilateral labyrinthectomy on GAD, GATI and GABA receptor gene expression in the rat vestibular nucleus 査読

    A Horii, T Kitahara, PF Smith, CL Darlington, C Masumura, T Kubo

    NEUROREPORT   14 ( 18 )   2359 - 2363   2003年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    To elucidate the role of the GABAergic neuronal system in the recovery from peripheral vestibular damage (unilateral labyrinthectomy), we used a real-time quantitative reverse transcription-polymerase chain reaction method to investigate the mRNA expression of GAD65, GAD67, the GABA(A) receptor alpha1 subunit, the GABA(B) R1 subunit, and the GABA transporter GATI, in the vestibular nucleus complex of the rat 6 and 50 h following the lesion GAD65 and GAD67 gene expression were also measured in the flocculus. The GABAA alpha1 subunit mRNA was up-regulated in the ipsilateral vestibular nucleus 6 h post-lesion but decreased in expression thereafter. GAD65 mRNA was up-regulated in the vestibular nuclei bilaterally 50 h after the lesion. In the flocculus, GAD65 mRNA expression was bilaterally up-regulated 50 h post-operatively. GATI mRNA expression was initially up-regulated in the ipsilateral vestibular nucleus and then underwent a bilateral increase 50h post-operatively. These results demonstrate that following unilateral labyrinthectomy, major changes in the expression of GAD, GAT and GABA receptor subunit genes occur in the vestibular nucleus, which are likely to affect the process of behavioural recovery. (C) 2003 Lippincott Williams Wilkins.

    DOI: 10.1097/01.wnr.0000102266.71579.6c

    Web of Science

    PubMed

    researchmap

  • Magnetic resonance imaging of pleomorphic adenoma arising from the external auditory canal. 査読

    Masumura C, Horii A, Mishiro Y, Inohara H, Kitahara T, Takashima S, Kubo T

    The Journal of laryngology and otology   117 ( 11 )   908 - 9   2003年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1258/002221503322542980

    PubMed

    researchmap

  • Fos induction in the amygdala by vestibular information during hypergravity stimulation 査読

    A Nakagawa, A Uno, A Horii, T Kitahara, M Kawamoto, Y Uno, M Fukushima, S Nishiike, N Takeda, T Kubo

    BRAIN RESEARCH   986 ( 1-2 )   114 - 123   2003年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Altered gravity environments including both hypo- and hypergravity can elicit motion sickness. Vestibular information is known to be essential for motion sickness, but its other neural substrates are poorly understood. We previously showed that bilateral lesions of the amygdala suppressed hypergravity-induced motion sickness in rats, using pica behavior as an emetic index. We show in the present study that during hypergravity stimulation, vestibular information activated the central nucleus of the amygdala (CeA), as determined by the induction of Fos expression, in comparison between normal and bilaterally labyrinthectomized rats. The finding that Fos expression was confined to the CeA and almost completely absent in other subnuclei of the amygdala contrasted with many previous studies that used other stressful stimuli such as foot shock, restraint and forced swimming, suggesting a specific vestibular effects on the amygdala. Prolongation of hypergravity resulted in reduction of Fos expression in the CeA, suggesting a process of habituation. Such decreases appeared earlier than in the vestibular nucleus, suggesting that adaptive changes in the CeA to hypergravity were independent of changes in the vestibular input. Our results suggest the amygdala is a neural substrate involved in the development of and habituation to motion sickness. (C) 2003 Elsevier B.V. All rights reserved.

    DOI: 10.1016/S0006-8993(03)03220-7

    Web of Science

    PubMed

    researchmap

  • Long-term effects of permanent vestibular lesions on hippocampal spatial firing. 査読

    Russell NA, Horii A, Smith PF, Darlington CL, Bilkey DK

    The Journal of neuroscience : the official journal of the Society for Neuroscience   23 ( 16 )   6490 - 8   2003年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PubMed

    researchmap

  • Steroid effects on vestibular compensation in human. 査読

    Kitahara T, Kondoh K, Morihana T, Okumura S, Horii A, Takeda N, Kubo T

    Neurological research   25 ( 3 )   287 - 91   2003年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1179/016164103101201364

    PubMed

    researchmap

  • Horizontal canal type BPPV: Bilaterally affected case treated with canal plugging and Lempert's maneuver 査読

    A Horii, T Imai, Y Mishiro, Y Yamaji, K Mitani, T Kawashima, T Kubo

    ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY AND ITS RELATED SPECIALTIES   65 ( 6 )   366 - 369   2003年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    A 54-year-old woman complained of positional vertigo. During 3 months' observation, the patient showed mostly geotropic or apogeotropic nystagmus due to right canalolithiasis or cupulolithiasis, however, she sometimes showed nystagmus which suggested left horizontal canalolithiasis. We suspected that she suffered from bilateral horizontal canal type benign paroxysmal positional vertigo (BPPV) and performed Lempert's maneuver for both directions, however, they were ineffective. She underwent canal plugging for right horizontal canal. After surgery she showed no positional nystagmus of right horizontal canal origin. However, apogeotropic nystagmus of the left horizontal canal origin was still observed. This nystagmus changed to geotropic nystagmus and finally disappeared following Lempert's maneuver for the left side. Bilateral horizontal canal BPPV is difficult to be resolved, probably because physical treatment for one side would move debris to the cupula in the other canal. Canal plugging combined with Lempert's maneuver to the other side is one treatment option for intractable bilateral horizontal canal BPPV. Copyright (C) 2003 S. Karger AG, Basel.

    DOI: 10.1159/000076056

    Web of Science

    PubMed

    researchmap

  • Bilateral peripheral vestibular lesions produce long-term changes in spatial learning in the rat 査読

    NA Russell, A Horii, PF Smith, CL Darlington, DK Bilkey

    JOURNAL OF VESTIBULAR RESEARCH-EQUILIBRIUM & ORIENTATION   13 ( 1 )   9 - 16   2003年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IOS PRESS  

    In order to investigate whether bilateral peripheral vestibular lesions cause long-term impairment of spatial learning, rats were tested in a reference memory radial arm maze learning task at least 5 weeks following a bilateral labyrinthectomy (BL) or sham control lesion. All control rats reached criterion (i.e., 1 error or less, averaged across 7 trials for 3 consecutive days of training) but only 4 of the 8 BL rats had reached criterion by day 21 of the training sessions. The control rats reached criterion more quickly than the lesioned rats (Control, 7.0 +/- 0.63 days, Lesioned, 15.8 +/- 1.4 days, t(10) = 5.84, p < 0.0001). This difference resulted from the greater number of errors made by the BL animals. However, the latency to respond was comparable as a result of the increased locomotor activity of the BL group (i.e., 'hyperkinesis), and the overall rate of acquisition of the task, as indicated by analysis of the exponential decrease in errors over the entire training period, was not significantly different between the 2 groups. The results of this study demonstrate that BL in rats produces long-term changes in performance in a spatial reference memory task, which are not simply due to the inability to move but may relate to the way that the brain uses vestibular information to create spatial representations and determines behavioural strategies on the basis of these representations.

    Web of Science

    PubMed

    researchmap

  • Changes in fos expression in the rat brainstem after bilateral labyrinthectomy. 査読

    Kitahara T, Nakagawa A, Fukushima M, Horii A, Takeda N, Kubo T

    Acta oto-laryngologica   122 ( 6 )   620 - 6   2002年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/000164802320396295

    PubMed

    researchmap

  • Quantitative changes in mRNA expression of glutamate receptors in the rat peripheral and central vestibular systems following hypergravity. 査読

    Uno Y, Horii A, Uno A, Fuse Y, Fukushima M, Doi K, Kubo T

    Journal of neurochemistry   81 ( 6 )   1308 - 17   2002年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1046/j.1471-4159.2002.00933.x

    PubMed

    researchmap

  • [Changes in plasma inner ear hormones after endolymphatic sac drainage and steroid-instillation surgery (EDSS)]. 査読

    Kitahara T, Horii A, Mishiro Y, Fukushima M, Kondoh K, Okumura S, Takeda N, Kubo T

    Nihon Jibiinkoka Gakkai kaiho   105 ( 5 )   557 - 63   2002年5月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3950/jibiinkoka.105.5_557

    PubMed

    researchmap

  • Application of real-time quantitative polymerase chain reaction to quantification of glutamate receptor gene expression in the vestibular brainstem and cerebellum 査読

    A Horii, PF Smith, CL Darlington

    BRAIN RESEARCH PROTOCOLS   9 ( 1 )   77 - 83   2002年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Reverse transcription-polymerase chain reaction (RT-PCR) is a powerful tool to detect specific gene expression from a small amount of tissue, which is superior to the traditional RNA assays such as Northern blotting and in situ hybridization (ISH) in terms of sensitivity. However, conventional RT-PCR is not suitable for quantification due to its exponential nature. Recently, a real-time quantitative PCR method has been developed to overcome the weak points of RT-PCR, e.g. quantification. Here we describe the use of real-time quantitative PCR using a fluorescent TaqMan probe, to study the regional differences in expression of glutamate receptor subunit/subtype genes (NR1, NR2A, GluR2, KA2, mGluR1, mGluR7) in the central vestibular system including the vestibular nucleus complex, inferior olive and cerebellar flocculus. We found that real-time quantitative PCR yielded similar results to other techniques such as ISH but offered several advantages in terms of relative speed and ability to detect low levels of gene expression. We suggest that real-time quantitative PCR is a useful method to study gene expression for other neurotransmitter receptors in the vestibular brainstem and cerebellum, and is also expected to be more accurate to assess the changes in gene expression following any treatment. (C) 2002 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S1385-299X(01)00139-8

    Web of Science

    PubMed

    researchmap

  • Ki-67 positive fractions in benign and malignant thyroid tumours: Application of flow cytometry 査読

    A Horii, JI Yoshida, M Sakai, S Okamoto, Y Honjo, K Mitani, K Hattori, T Kubo

    ACTA OTO-LARYNGOLOGICA   119 ( 5 )   617 - 620   1999年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SCANDINAVIAN UNIVERSITY PRESS  

    We investigated the DNA ploidy pattern. cell cycle and the percentage of Ki-67 positive fractions in Fresh surgical material from 17 benign and 33 malignant thyroid tumours using flow cytometry. DNA aneuploidy was not seen at all in benign tumours. but was seen in 3 out of 33 malignant rumours. suggesting that detection of DNA,4 aneuploidy indicates malignancy, although the detection sensitivity was low. Regarding the cell cycle. there was no difference in the percentage of S-phase fractions (SPF) or G2 plus M phase fractions (G2M) between benign and malignant rumours. However. the percentage of Ki-69 positive fractions in malignant rumours (39.9 +/- 3.9) was significantly higher than that in benign rumours (9.4 +/- 2.1%,), indicating that malignant thyroid rumours contained a large population of G1 phase cells. When a cut-off value of 20%; was used for Ki-67 positive Fractions. sensitivity was 82%;, specificity was 88% and accuracy was 84% fur the diagnosis of malignant tumours. Although this study was carried our on surgically derived marerials, it is possible that flow cytometric analysis of fine needle aspiration-derived materials may have a place in preoperative histopathological assessment of thyroid tumours.

    DOI: 10.1080/00016489950180883

    Web of Science

    researchmap

  • Effect of unilateral vestibular stimulation on histamine release from the hypothalamus of rats in vivo 査読

    A. Horii, N. Takeda, T. Matsunaga, A. Yamatodani, T. Mochizuki, K. Okakura- Mochizuki, H. Wada

    Journal of Neurophysiology   70 ( 5 )   1822 - 1826   1993年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    1. We investigated the effect of unilateral vestibular stimulation on histamine release from the anterior hypothalamic area of urethan-anesthetized rats in vivo, using a brain microdialysis method coupled with high- performance liquid chromatography fluorometry. 2. The histamine release was increased to ~180% of the basal release by the electrical stimulation of the inner ear with 1 Hz, 500 μA, and 200 ms for 20 min. This effect was dependent on the current intensity. 3. Activation of the unilateral horizontal semicircular canal by middle ear irrigation for 15 min with 45°C water increased the histamine release to ~200% of the basal release. 4. Irrigation of the middle ear with ice water for 15 min increased the histamine release to ~190% of the basal release. 5. The histamine release was not changed by the irrigation of the middle ear with 37°C water and the irrigation of the auricle with ice water, which suggests that neither somatosensory stimulation to the middle ear nor nonspecific cold stress affects the histamine release. 6. All these findings suggest that the sensory mismatch signals induced by caloric stimulation and unilateral electrical vestibular stimulation activate the histaminergic neuron system in the brain.

    DOI: 10.1152/jn.1993.70.5.1822

    Scopus

    PubMed

    researchmap

▶ 全件表示

 

担当経験のある授業科目

  • 医学序説 I

    2021年
    -
    現在
    機関名:新潟大学

  • 医学序説 II

    2020年
    -
    現在
    機関名:新潟大学

  • 臨床医学講義(集中)

    2020年
    -
    現在
    機関名:新潟大学

  • 臓器別講義・演習Ⅲ

    2020年
    -
    現在
    機関名:新潟大学

  • 耳鼻頭頚

    2017年
    機関名:新潟大学