2024/10/06 更新

写真a

ハヤツ マナブ
早津 学
HAYATSU Manabu
所属
教育研究院 医歯学系 医学系列 助教
医歯学総合研究科 分子細胞医学専攻 細胞機能 助教
職名
助教
外部リンク

学位

  • 博士(理学) ( 2015年9月   神奈川大学 )

研究キーワード

  • 透過型電子顕微鏡

研究分野

  • ライフサイエンス / 解剖学  / 電子顕微鏡

経歴

  • 新潟大学   医歯学総合研究科 分子細胞医学専攻 細胞機能   助教

    2016年4月 - 現在

 

論文

  • Inductive effect of SORT1 on odontoblastic differentiation of human dental pulp-derived stem cells

    Daisuke Omagari, Taku Toriumi, Hiromasa Tsuda, Manabu Hayatsu, Keisuke Watanabe, Yusuke Mizutami, Masaki Honda, Yoshikazu Mikami

    Differentiation   133   88 - 97   2023年9月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.diff.2023.08.001

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  • Spatiotemporal expression patterns of R-spondins and their receptors, Lgrs, in the developing mouse telencephalon. 国際誌

    Keisuke Watanabe, Masao Horie, Manabu Hayatsu, Yoshikazu Mikami, Noboru Sato

    Gene expression patterns : GEP   49   119333 - 119333   2023年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Development of the mammalian telencephalon, which is the most complex region of the central nervous system, is precisely orchestrated by many signaling molecules. Wnt signaling derived from the cortical hem, a signaling center, is crucial for telencephalic development including cortical patterning and the induction of hippocampal development. Secreted protein R-spondin (Rspo) 1-4 and their receptors, leucine-rich repeat-containing G-protein-coupled receptor (Lgr) 4-6, act as activators of Wnt signaling. Although Rspo expression in the hem during the early stages of cortical development has been reported, comparative expression analysis of Rspos and Lgr4-6 has not been performed. In this study, we examined the detailed spatiotemporal expression patterns of Rspo1-4 and Lgr4-6 in the embryonic and postnatal telencephalon to elucidate their functions. In the embryonic day (E) 10.5-14.5 telencephalon, Rspo1-3 were prominently expressed in the cortical hem. Among their receptors, Lgr4 was observed in the ventral telencephalon, and Lgr6 was highly expressed throughout the telencephalon at the same stages. This suggests that Rspo1-3 and Lgr4 initially regulate telencephalic development in restricted regions, whereas Lgr6 functions broadly. From the late embryonic stage, the expression areas of Rspo1-3 and Lgr4-6 dramatically expanded; their expression was found in the neocortex and limbic system, such as the hippocampus, amygdala, and striatum. Increased Rspo and Lgr expression from the late embryonic stages suggests broad roles of Rspo signaling in telencephalic development. Furthermore, the Lgr+ regions were located far from the Rspo+ regions, especially in the E10.5-14.5 ventral telencephalon, suggesting that Lgrs act via a Rspo-independent pathway.

    DOI: 10.1016/j.gep.2023.119333

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  • miR-92a-3p encapsulated in bone metastatic mammary tumor cell-derived extracellular vesicles modulates mature osteoclast longevity. 査読 国際誌

    Norihisa Uehara, Yukari Kyumoto-Nakamura, Yoshikazu Mikami, Manabu Hayatsu, Soichiro Sonoda, Takayoshi Yamaza, Akiko Kukita, Toshio Kukita

    Cancer science   113 ( 12 )   4219 - 4229   2022年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aberrant osteoclast formation and activation are the hallmarks of osteolytic metastasis. Extracellular vesicles (EVs), released from bone metastatic tumor cells, play a pivotal role in the progression of osteolytic lesions. However, the mechanisms through which tumor cell-derived EVs regulate osteoclast differentiation and function have not been fully elucidated. In this study, we found that 4T1 bone metastatic mouse mammary tumor cell-derived EVs (4T1-EVs) are taken up by mouse bone marrow macrophages to facilitate osteoclastogenesis. Furthermore, treatment of mature osteoclasts with 4T1-EVs promoted bone resorption, which was accompanied by enhanced survival of mature osteoclasts through the negative regulation of caspase-3. By comparing the miRNA content in 4T1-EVs with that in 67NR nonmetastatic mouse mammary tumor cell-derived EVs (67NR-EVs), miR-92a-3p was identified as one of the most enriched miRNAs in 4T1-EVs, and its transfer into mature osteoclasts significantly reduced apoptosis. Bioinformatic and Western blot analyses revealed that miR-92a-3p directly targeted phosphatase and tensin homolog (PTEN) in mature osteoclasts, resulting in increased levels of phospho-Akt. Our findings provide novel insights into the EV-mediated regulation of osteoclast survival through the transfer of miR-92a-3p, which enhances mature osteoclast survival via the Akt survival signaling pathway, thus promoting bone resorption.

    DOI: 10.1111/cas.15557

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  • Diet-related changes of basal lamina fenestrations in the villous epithelium of the rat small intestine: Statistical analysis on scanning electron microscopy.

    Masatoshi Sato, Keisuke Morita, Rie Azumi, Yusuke Mizutani, Manabu Hayatsu, Tatsuo Ushiki, Shuji Terai

    Biomedical research (Tokyo, Japan)   43 ( 1 )   11 - 22   2022年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The epithelial basal lamina of the small intestine has numerous fenestrations for intraepithelial migration of leukocytes. We have reported dynamic changes of fenestrations in dietary conditions. To investigate this phenomenon, we performed statistical analyses using scanning electron microscopy images of the epithelial basal lamina of rat intestinal villi after removal of the villous epithelium by osmium maceration. We examined structural changes in the number and size of fenestrations in the rat jejunum and ileum under fasted and fed states for 24 h. Our findings revealed that, in the jejunum, the number of free cells migrating into the epithelium through fenestrations increased from 2 h after feeding, resulting in an increase in the fenestration size of intestinal villi; the number of free cells then tended to decrease at 6 h after feeding, and the fenestration size also gradually decreased. By contrast, the increase in the fenestration size by feeding was not statistically significant in the ileum. These findings indicate that the number of migrating cells increases in the upper part of the small intestine under dietary conditions, which may influence the absorption efficiency of nutrients including lipids, as well as the induction of nutrient-induced inflammation.

    DOI: 10.2220/biomedres.43.11

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  • Rice peptide with amino acid substitution inhibits biofilm formation by Porphyromonas gingivalis and Fusobacterium nucleatum

    Aoi Matsugishi, Yukari Aoki-Nonaka, Mai Yokoji-Takeuchi, Miki Yamada-Hara, Yoshikazu Mikami, Manabu Hayatsu, Yutaka Terao, Hisanori Domon, Masayuki Taniguchi, Naoki Takahashi, Kazuhisa Yamazaki, Koichi Tabeta

    Archives of Oral Biology   121   104956 - 104956   2021年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.archoralbio.2020.104956

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  • SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role. 査読 国際誌

    Yusuke Mizutani, Daisuke Omagari, Manabu Hayatsu, Masaaki Nameta, Kazuo Komiyama, Yoshikazu Mikami, Tatsuo Ushiki

    Cell adhesion & migration   14 ( 1 )   195 - 203   2020年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To elucidate the underlying mechanism of secretory leukocyte protease inhibitor (SLPI)-induced cell migration, we compared SLPI-deleted human gingival carcinoma Ca9-22 (ΔSLPI) cells and original (wild-type: wt) Ca9-22 cells using several microscopic imaging methods and gene expression analysis. Our results indicated reduced migration of ΔSLPI cells compared to wtCa9-22 cells. The lamellipodia/dorsal ruffles were smaller and moved slower in ΔSLPI cells compared to wtCa9-22 cells. Furthermore, well-developed intermediate filament bundles were observed at the desmosome junction of ΔSLPI cells. In addition, Galectin4 was strongly expressed in ΔSLPI cells, and its forced expression suppressed migration of wtCa9-22 cells. Taken together, SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.

    DOI: 10.1080/19336918.2020.1829264

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  • Gap junction with MLO-A5 osteoblast-like cell line induces ALP and BSP transcription of 3T3-L1 pre-adipocyte like cell line via Hspb1 while retaining adipogenic differentiation ability. 査読 国際誌

    Daisuke Omagari, Manabu Hayatsu, Kiyofumi Yamamoto, Masayuki Kobayashi, Naruchika Tsukano, Masaaki Nameta, Yoshikazu Mikami

    Bone   141   115596 - 115596   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In bone tissues, gap junctions form direct links between the cytoplasm of an osteocyte and another adjacent osteocyte or osteoblast, which underlie both bone formation and bone resorption. We have previously demonstrated that alkaline phosphatase (ALP) and bone sialoprotein (BSP), which are osteoblast markers, were induced in mesenchymal stem cells (MSCs) co-cultured with osteoblast-like cell line. However, the molecular mechanism of this process has not been fully addressed. Furthermore, few advances have been made toward elucidating the communication networks that link the status of committed cells such as (pre-) adipocytes that differentiated from MSCs as well as osteoblasts. Therefore, the objective of the present study was to investigate the mechanism underlying the communication network between pre-adipocytes and osteoblasts. We evaluated the effect of co-culture with osteoblast on the cell status of pre-adipocytes using murine osteoblast-like cell line, MLO-A5, and pre-adipocyte-like cell line, 3T3-L1, respectively. The results presented here demonstrated that osteoblasts and pre-adipocytes communicate via gap junctions, and the ensuing drastic increase in ALP and BSP transcription in co-cultured pre-adipocytes was induced, at least partly, via heat shock protein family B member 1 (Hspb1). In addition, terminal differentiation into adipocytes was suppressed in pre-adipocytes during co-culture with osteoblast without loss of adipogenic differentiation ability. Interestingly, after co-culture with osteoblasts, isolated co-cultured pre-adipocytes were able to differentiate to adipocytes as well as original pre-adipocytes. These results suggest that gap junctional communication with osteoblasts suppressed adipogenic differentiation of pre-adipocytes without loss of adipogenic differentiation ability.

    DOI: 10.1016/j.bone.2020.115596

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  • Effect of methionine/choline-deficient diet and high-fat diet-induced steatohepatitis on mitochondrial homeostasis in mice. 査読 国際誌

    Yoshihisa Arao, Hirokazu Kawai, Kenya Kamimura, Takamasa Kobayashi, Oki Nakano, Manabu Hayatsu, Tatsuo Ushiki, Shuji Terai

    Biochemical and biophysical research communications   527 ( 2 )   365 - 371   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Considering the increase in cases of non-alcoholic steatohepatitis (NASH), the use of appropriate animal model of NASH is essential to understand the underlying pathogenesis mechanism. To date, several mice models have been used; however, significant differences in the etiologies and food administered affected the results, with inconsistent conclusions. Therefore, it is necessary to understand these models and their differences to be able to choose appropriate models. Inspired by the fact that mitochondrial (mt)DNA content is changed in non-alcoholic fatty liver disease in humans, we investigated the mtDNA copy number in the NASH mice models induced by high-fat diet (HFD) and methionine/choline-deficient diet (MCD) to understand the differences between these models. Megamitochondria were observed in both MCD and HFD groups. However, the MCD group showed significant decrease in liver mtDNA content compared with that in the HFD group. These changes were associated with significant upregulation of mitochondrial biogenesis- and degradation-related genes in MCD model than in HFD model. Thus, stability of mtDNA is associated with the differences between MCD and HFD-induced NASH models often used in studies; these findings could help in choosing appropriate models for studies on NASH.

    DOI: 10.1016/j.bbrc.2020.03.180

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  • コメペプチドとそのアミノ酸置換体はPorphyromonas gingivalis、Fusobacterium nucleatumのバイオフィルム形成を阻害する

    松岸 葵, 野中 由香莉, 竹内 麻衣, 原 実生, 早津 学, 三上 剛和, 牛木 辰男, 土門 久哲, 山崎 和久, 多部田 康一

    日本歯周病学会会誌   62 ( 春季特別 )   133 - 133   2020年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • コメペプチドとそのアミノ酸置換体はPorphyromonas gingivalis、Fusobacterium nucleatumのバイオフィルム形成を阻害する

    松岸 葵, 野中 由香莉, 竹内 麻衣, 原 実生, 早津 学, 三上 剛和, 牛木 辰男, 土門 久哲, 山崎 和久, 多部田 康一

    日本歯周病学会会誌   62 ( 春季特別 )   133 - 133   2020年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • Dynamic changes in basal lamina fenestrations in rat intestinal villous epithelium under high-fat diet condition. 査読

    Keisuke Morita, Rie Azumi, Masatoshi Sato, Yusuke Mizutani, Manabu Hayatsu, Shuji Terai, Tatsuo Ushiki

    Biomedical research (Tokyo, Japan)   40 ( 2 )   57 - 66   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The basal lamina of the villous epithelium in the small intestine has numerous fenestrations, which are produced by leukocytes for their intraepithelial migration. We previously showed that these fenestrations change due to the dynamics of migrating leukocytes in response to dietary conditions and suggested the possibility that this change is related to the regulation of the absorption of large-sized nutrients such as chylomicrons. The present study was, thus, designed to investigate structural changes in basal lamina fenestrations in response to a high-fat diet. The ultrastructure of the intestinal villi in the rat upper jejunum was investigated by electron microscopy of tissue sections in both the normal and the high-fat diet groups, and the fenestrations in the villous epithelium of rat upper jejunum were studied by scanning electron microscopy of osmium macerated/ ultrasonicated tissues. The present study showed that free cells adhering to the fenestrations increased in the upper jejunum two hours after feeding high-fat diet and the size of the fenestrations in this region also increased after feeding high-fat diet for 2 days. This enlargement of fenestrations may play an important role in increasing the efficiency of lipid absorption by facilitating the movement of chylomicrons from the intercellular space to the lamina propria.

    DOI: 10.2220/biomedres.40.57

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  • Dual effect of polyphosphate on mineralization of rat osteoblast ROS17/2.8 cells in a dose-dependent manner. 査読

    Mikami Y, Omagari D, Mizutani Y, Hayatsu M, Ushiki T, Tsuda H

    Journal of pharmacological sciences   138 ( 3 )   209 - 213   2018年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jphs.2018.10.002

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  • A bacterial metabolite ameliorates periodontal pathogen-induced gingival epithelial barrier disruption via GPR40 signaling. 査読 国際誌

    Miki Yamada, Naoki Takahashi, Yumi Matsuda, Keisuke Sato, Mai Yokoji, Benso Sulijaya, Tomoki Maekawa, Tatsuo Ushiki, Yoshikazu Mikami, Manabu Hayatsu, Yusuke Mizutani, Shigenobu Kishino, Jun Ogawa, Makoto Arita, Koichi Tabeta, Takeyasu Maeda, Kazuhisa Yamazaki

    Scientific reports   8 ( 1 )   9008 - 9008   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nature Publishing Group  

    Several studies have demonstrated the remarkable properties of microbiota and their metabolites in the pathogenesis of several inflammatory diseases. 10-Hydroxy-cis-12-octadecenoic acid (HYA), a bioactive metabolite generated by probiotic microorganisms during the process of fatty acid metabolism, has been studied for its protective effects against epithelial barrier impairment in the intestines. Herein, we examined the effect of HYA on gingival epithelial barrier function and its possible application for the prevention and treatment of periodontal disease. We found that GPR40, a fatty acid receptor, was expressed on gingival epithelial cells; activation of GPR40 by HYA significantly inhibited barrier impairment induced by Porphyromonas gingivalis, a representative periodontopathic bacterium. The degradation of E-cadherin and beta-catenin, basic components of the epithelial barrier, was prevented in a GPR40-dependent manner in vitro. Oral inoculation of HYA in a mouse experimental periodontitis model suppressed the bacteria-induced degradation of E-cadherin and subsequent inflammatory cytokine production in the gingival tissue. Collectively, these results suggest that HYA exerts a protective function, through GPR40 signaling, against periodontopathic bacteria-induced gingival epithelial barrier impairment and contributes to the suppression of inflammatory responses in periodontal diseases.

    DOI: 10.1038/s41598-018-27408-y

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  • Dynamics of basal lamina fenestrations in the rat intestinal villous epithelium in response to dietary conditions. 査読

    Rie Azumi, Keisuke Morita, Yusuke Mizutani, Manabu Hayatsu, Shuji Terai, Tatsuo Ushiki

    Biomedical research (Tokyo, Japan)   39 ( 2 )   65 - 74   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Biomedical Research Foundation  

    The epithelial basal lamina of the small intestine forms a felt-like sheet at the base of the epithelium. Previous studies have shown that the basal lamina has numerous fenestrations, which are produced by leukocytes penetrating through the basal lamina. In this study, we aimed to directly visualize fenestrations of the rat basal lamina in intestinal villi by scanning electron microscopy (SEM) after removal of the villous epithelium by osmium maceration and ultrasonic treatment. Structural changes in fenestrations were then investigated in relation to dietary conditions. SEM of these tissues revealed the presence of fenestrations in the villous epithelial basal lamina in all segments of the small intestine, although the number was the highest in the jejunum. The present study also showed that the number and size of fenestrations increased after feeding in the jejunum, whereas changes were unclear in the ileum. These findings suggested that the basal lamina fenestrations were changed through the dynamics of migrating leukocytes in dietary conditions and may also be related to the regulation of nutrient absorption, particularly as lipids are transported from the intercellular space of the epithelium to the lamina propria.

    DOI: 10.2220/biomedres.39.65

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  • Protonema of the moss Funaria hygrometrica can function as a lead (Pb) adsorbent. 査読 国際誌

    Misao Itouga, Manabu Hayatsu, Mayuko Sato, Yuuri Tsuboi, Yukari Kato, Kiminori Toyooka, Suechika Suzuki, Seiji Nakatsuka, Satoshi Kawakami, Jun Kikuchi, Hitoshi Sakakibara

    PloS one   12 ( 12 )   e0189726   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science  

    Water contamination by heavy metals from industrial activities is a serious environmental concern. To mitigate heavy metal toxicity and to recover heavy metals for recycling, biomaterials used in phytoremediation and bio-sorbent filtration have recently drawn renewed attention. The filamentous protonemal cells of the moss Funaria hygrometrica can hyperaccumulate lead (Pb) up to 74% of their dry weight when exposed to solutions containing divalent Pb. Energy-dispersive X-ray spectroscopy revealed that Pb is localized to the cell walls, endoplasmic reticulum-like membrane structures, and chloroplast thylakoids, suggesting that multiple Pb retention mechanisms are operating in living F. hygrometrica. The main Pb-accumulating compartment was the cell wall, and prepared cell-wall fractions could also adsorb Pb. Nuclear magnetic resonance analysis showed that polysaccharides composed of polygalacturonic acid and cellulose probably serve as the most effective Pb-binding components. The adsorption abilities were retained throughout a wide range of pH values, and bound Pb was not desorbed under conditions of high ionic strength. In addition, the moss is highly tolerant to Pb. These results suggest that the moss F. hygrometrica could be a useful tool for the mitigation of Pb-toxicity in wastewater.

    DOI: 10.1371/journal.pone.0189726

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  • Three-dimensional reconstruction of root cells and interdental cells in the rat inner ear by serial section scanning electron microscopy. 査読

    Ryusuke Shodo, Manabu Hayatsu, Daisuke Koga, Arata Horii, Tatsuo Ushiki

    Biomedical research (Tokyo, Japan)   38 ( 4 )   239 - 248   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    In the cochlea, a high K+ environment in the endolymph is essential for the maintenance of normal hearing function, and the transport of K+ ions through gap junctions of the cochlear epithelium is thought to play an important role in endolymphatic homeostasis. The aim of the present study was to demonstrate the three-dimensional (3D) ultrastructure of spiral ligament root cells and interdental cells, which are located at both ends of the gap junction system of the cochlea epithelium. Serial semi-thin sections of plastic-embedded rat cochlea were mounted on glass slides, stained with uranyl acetate and lead citrate, and observed by scanning electron microscopy (SEM) using the backscattered electron (BSE) mode. 3D reconstruction of BSE images of serial sections revealed that the root cells were linked together to form a branched structure like an elaborate "tree root" in the spiral ligament. The interdental cells were also connected to each other, forming a comb-shaped cellular network with a number of cellular strands in the spiral limbus. Furthermore, TEM studies of ultra-thin sections revealed the rich presence of gap junctions in both root cells and interdental cells. These findings suggest the possibility that both root cells and interdental cells contribute to K+ circulation as the end portion of the epithelial cell gap junction system of the cochlea.

    DOI: 10.2220/biomedres.38.239

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  • Electron probe X-ray microanalysis studies on the distribution change of intra- and extracellular calcium in the elongation zone of horizontally reoriented soybean roots. 査読 国際誌

    Manabu Hayatsu, Suechika Suzuki

    Microscopy (Oxford, England)   64 ( 5 )   327 - 34   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    To clarify the contribution of Ca to the gravitropic response, quantitative X-ray microanalyses were performed on cryosections of roots of soybean seedlings reoriented horizontally from their original vertical orientation. After reorientation, the roots bent gradually toward the ground at the elongation zone. The concentrations of Ca in the cell walls, cytoplasmic matrices and central vacuoles of cortical cells were measured in the upper and lower halves of the elongation zone at 0, 30, 60 and 120 min after reorientation. The Ca concentration did not significantly change in the cytoplasmic matrices or vacuoles. Additionally, the Ca concentration did not change significantly in cell walls at 30 min after reorientation; however, beyond 30 min, this concentration significantly increased gradually in the lower half of the elongation zone and decreased in the upper half of the elongation zone, indicating a typical asymmetrical distribution of Ca. These results suggest that Ca moves apoplastically in soybean roots to produce an asymmetrical Ca distribution in the elongation zone, which contributes to root curvature. The possible role of Ca in accelerating or repressing the effect of auxin is also discussed in this study.

    DOI: 10.1093/jmicro/dfv031

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  • Effect of NaCl on ionic content and distribution in suspension-cultured cells of the halophyte Sonneratia alba versus the glycophyte Oryza sativa. 査読 国際誌

    Manabu Hayatsu, Suechika Suzuki, Ai Hasegawa, Shinpei Tsuchiya, Hamako Sasamoto

    Journal of plant physiology   171 ( 15 )   1385 - 91   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    The effect of a high concentration of NaCl on the intra- (cytoplasmic matrix and vacuole) and extracellular (cell wall) distribution of Na, Cl, K, Mg, Ca, S, and P was investigated in suspension-cultured cells of the mangrove halophyte Sonneratia alba and compared to cultured cells of glycophytic rice (Oryza sativa). No significant differences were observed in ultrastructural features of cluster cells of both species cultured with and without 50mM NaCl. Quantitative X-ray microanalysis of cryosections of the cells cultured in the presence of 50mM NaCl showed that the Na concentration ([Na]) and Cl concentration ([Cl]) significantly increased in all three cell components measured. In S. alba, the [Na] was highest in the vacuole and lowest in the cytoplasmic matrix, while the [Cl] was highest in the cell wall and lowest in the cytoplasmic matrix. In O. sativa, however, the [Na] and [Cl] were highest in the cell wall, and the [Na] was lowest in the cytoplasmic matrix. Thus, the possible activities for Na and Cl transport from the cytoplasmic matrix into the vacuole were greater in S. alba than in O. sativa, suggesting that halophilic mangrove cells gain salt tolerance by transporting Na and Cl into their vacuoles. In O. sativa, the addition of NaCl to the culture medium caused no significant changes to the intracellular concentrations of various elements, such as K, P, S, Ca, and Mg, which suggests the absence of a direct relationship with the transport Na and Cl. In contrast, a marked decrease in the Ca concentration ([Ca]) in the cytoplasmic matrix and vacuole and an approximately two-fold increase in the P concentration ([P]) in the cytoplasmic matrix were found in S. alba, suggesting that the decrease in the [Ca] is related to the halophilic nature of S. alba (as indicated by the inward movement of Na(+) and Cl(-)). The possible roles of a Na(+)/Ca(2+) exchange mechanism in halophilism and the effect of the [P] on the metabolic activity under saline conditions are discussed.

    DOI: 10.1016/j.jplph.2014.06.008

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  • Properties of lead deposits in cell walls of radish (Raphanus sativus) roots. 査読

    Hiroshi Inoue, Daisuke Fukuoka, Yuri Tatai, Hiroyuki Kamachi, Manabu Hayatsu, Manami Ono, Suechika Suzuki

    Journal of plant research   126 ( 1 )   51 - 61   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER JAPAN KK  

    Various mechanisms are involved in detoxification of heavy metals such as lead (Pb) in plant cells. Most of the Pb taken up by plants accumulates in their roots. However, the detailed properties of Pb complexes in roots remain unclear. We have investigated the properties of Pb deposits in root cell walls of radish (Raphanus sativus L.) seedlings grown on glass beads bed containing Pb pellets, which are the source of Pb-contamination in shooting range soils. Pb deposits were tightly bound to cell walls. Cell wall fragments containing about 50,000 ppm Pb were prepared from the roots. After extracting Pb from the cell wall fragments using HCl, Pb ions were recombined with the Pb-extracted cell wall fragments in a solution containing Pb acetate. When the cell wall fragments were treated with pectinase (E.C. 3.2.1.15) and were chemically modified with 1-ethyl-3-dimethylamino-propylcarboimide, the Pb-rebinding ability of the treated cell wall fragments decreased. When acid-treated cell wall fragments were incubated in a solution containing Pb(2+) and excess amounts of a chelating agent, Pb recombined with the cell wall fragments were measured to estimate the affinity between Pb(2+) and the cell wall fragments. Our data show that Pb(2+) binds to carboxyl groups of cell walls. The source of the carboxyl groups is suggested to be pectic compounds. A stability constant of the Pb-cell wall complex was estimated to be about 10(8). The role of root cell walls in the mechanism underlying heavy metal tolerance was discussed.

    DOI: 10.1007/s10265-012-0494-6

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  • Cytochemical and electron probe X-ray microanalysis studies on the distribution change of intracellular calcium in columella cells of soybean roots under simulated microgravity. 査読

    Manabu Hayatsu, Manami Ono, Chieko Hamamoto, Suechika Suzuki

    Journal of electron microscopy   61 ( 1 )   57 - 69   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The columella cells of soybean roots grown under gravity and simulated microgravity induced by a clinostat were examined using potassium pyroantimonate (PA) and quantitative X-ray microanalysis of cryosections to determine the role of Ca in the regulation of the gravitropic response. Amyloplasts in the columella cells were localized exclusively at the bottom under gravity, but diffusely distributed in the cytoplasmic matrix under simulated microgravity, thus supporting the statolith theory. In the columella cells, PA precipitates containing Ca were diffusely distributed in the cytoplasmic matrix under gravity. Under simulated microgravity, however, they decreased in number and size in the cytoplasmic matrix, whereas increased only in number in the vacuole, indicating that Ca moved from the cytoplasmic matrix into the vacuole. The vacuole of columella cells contained mostly electron-dense granular structures localized along the inner surface of tonoplasts, which closely resembled the tannin vacuole reported in Mimosa pulvinar motor cells. Under simulated microgravity, their configuration changed dramatically from a granular shape to a flat plate. The quantitative X-ray microanalysis of cryosections showed that the vacuolar electron-dense structures contained a large amount of Ca. Under simulated microgravity, the concentration of Ca increased conspicuously in these vacuolar electron-dense structures, concomitantly with a marked decrease of K in the vacuoles and an increase of K in the cell walls. These results suggest that the release of Ca(2+) from, and uptake by, the vacuolar electron-dense structures is closely related to the signal transmission in the gravitropic response and that Ca movement occurs opposite to that of K.

    DOI: 10.1093/jmicro/dfr095

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MISC

  • コメペプチドとそのアミノ酸置換体はPorphyromonas gingivalis,Fusobacterium nucleatumのバイオフィルム形成を阻害する

    松岸葵, 松岸葵, 野中由香莉, 竹内麻衣, 原実生, 原実生, 早津学, 三上剛和, 牛木辰男, 土門久哲, 山崎和久, 多部田康一

    日本歯周病学会会誌(Web)   62   2020年

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共同研究・競争的資金等の研究

  • 液中環境における表面電荷分布のナノスケール可視化プローブ顕微鏡の開発

    研究課題/領域番号:19K22126

    2019年6月 - 2022年3月

    制度名:科学研究費助成事業

    研究種目:挑戦的研究(萌芽)

    提供機関:日本学術振興会

    岩田 太, 牛木 辰男, 早津 学

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    配分額:6500000円 ( 直接経費:5000000円 、 間接経費:1500000円 )

    液中環境での材料表面や生体細胞膜の表面といった固液界面の活性場において発現する現象は,電荷状態に直接的に関わっていることから,電荷分布の可視化はそこで生じる様々な現象の解明に極めて有効である.本研究は液中環境において試料表面の形状像と電荷分布像をナノスケールの分解能で可視化できるプローブ顕微鏡を開発した.走査型イオン伝導顕微鏡をベースに二つの開口を有するダブルバレルナノピペットをプローブとして用いた新奇なイオン電流検出法を開発することで実現した.本手法を用いて帯電処理したポリマー表面や染色体などの生体試料の電荷分布を可視化できることを実証し,その有効性を示した.

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  • 細胞分裂に伴う核膜と染色体の微細構造変化の電子顕微鏡技法を用いた三次元的解析

    研究課題/領域番号:19K16472

    2019年4月 - 2022年3月

    制度名:科学研究費助成事業

    研究種目:若手研究

    提供機関:日本学術振興会

    早津 学

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    配分額:4030000円 ( 直接経費:3100000円 、 間接経費:930000円 )

    本研究は細胞分裂中に生じる核膜、染色質、染色体の微細構造変化を三次元的に明らかにすることを目的とする。最初に核膜を裏打ちする蛋白質のラミンに赤色蛍光蛋白質が発現するHeLa細胞を作製した。次に、共焦点レーザー顕微鏡による観察とArray tomography法による三次元的解析で、体細胞分裂中期の細胞では、ラミンが存在しないことを明らかにした。また、TEMによる観察で細胞の赤道面に集まる染色体の周りには核膜に微細構造学的に類似している断片化した膜構造が存在することを明らかにした。これによって一般的に消失するとされる細胞分裂中期において核膜は完全に消失せず、残存している可能性を示すことができた。

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  • 小腸絨毛基底膜における「窓」の脂肪吸収に対する調節機能の解明

    研究課題/領域番号:19K08462

    2019年4月 - 2022年3月

    制度名:科学研究費助成事業

    研究種目:基盤研究(C)

    提供機関:日本学術振興会

    横山 純二, 寺井 崇二, 牛木 辰男, 高村 昌昭, 八木 一芳, 冨永 顕太郎, 佐藤 裕樹, 早津 学, 川田 雄三, 小島 雄一

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    配分額:4290000円 ( 直接経費:3300000円 、 間接経費:990000円 )

    肥満者にみられる十二指腸絨毛の白色変化を光学顕微鏡で観察すると、リンパ管拡張所見はみられず、細胞内や細胞間隙に蓄積したカイロミクロンを反映しているものと思われた。電子顕微鏡での観察結果と合わせ、小腸絨毛基底膜にみられる窓は、カイロミクロンの中心乳糜管への運搬を調整しており、肥満者においては、カイロミクロンの通過を厳しく制限している可能性が示唆された。すなわち、基底膜の窓は、高脂血症、肥満の抑制に寄与する方向に働いていると推察された。また、脂肪吸収の効率と基底膜の窓との相関については、腸管壁バリア機能の破綻や、小腸内細菌増殖症との関連も含め総合的に検討する必要があると考えられた。

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  • タンパク質分解酵素阻害因子(SLPI)によるがん病態の悪性化誘導機構の検討

    研究課題/領域番号:18K07226

    2018年4月 - 2021年3月

    制度名:科学研究費助成事業

    研究種目:基盤研究(C)

    提供機関:日本学術振興会

    三上 剛和, 上原 範久, 早津 学, 水谷 祐輔, 津田 啓方, 福島 敦史

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    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

    Secretory leukocyte protease inhibitor (SLPI) は,白血球が生産する酵素から,正常な皮膚や粘膜を保護するタンパク質である。その一方で,SLPIを発現するがん細胞は,「悪性がん細胞」であることが示唆されている。そこで,がん細胞の悪性化に対するSLPIの役割と作用機序を解明するために,SLPIを発現するがん細胞に対してSLPI遺伝子を欠損させて、その影響を解析した。その結果,SLPIはゲノムDNAを修飾することによって,細胞接着因子の発現を抑制し,がん細胞の移動能と浸潤能を亢進することを明らかにした。

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担当経験のある授業科目

  • 人体の構造と機能Ⅰ(組織学総論)

    2016年
    -
    2020年
    機関名:新潟大学

  • 人体の構造と機能Ⅱ(組織学各論)

    2016年
    -
    2020年
    機関名:新潟大学