Updated on 2024/10/06

写真a

 
MIYAZAKI Katsumi
 
Organization
Academic Assembly Institute of Science and Technology CHIKYU SEIBUTSU KAGAKU KEIRETU Professor
Faculty of Science Department of Science Professor
Title
Professor
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Degree

  • 博士(理学) ( 1992.3   筑波大学 )

Research Areas

  • Life Science / Aquatic life science

  • Life Science / Morphology and anatomical structure

  • Life Science / Biodiversity and systematics

Research History (researchmap)

  • Niigata University   Faculty of Science Department of Science   Professor

    2017.4

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  • Niigata University   Faculty of Science Department of Environmental Science Environmental Biology   Professor

    2016.7 - 2017.3

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  • Kyoto University   Field Science Education and Research Center   Lecturer

    2004.10 - 2016.6

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  • Kyoto University   Field Science Education and Research Center   Research Assistant

    2003.4 - 2004.10

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  • Kyoto University   Faculty of Science   Research Assistant

    1994.4 - 2003.3

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  • Keio University   Faculty of Law

    1992.4 - 1994.3

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Research History

  • Niigata University   Faculty of Science Department of Science   Professor

    2017.4

  • Niigata University   Abolition organization Environmental Biology   Professor

    2016.7 - 2017.3

Education

  • University of Tsukuba   Graduate School, Division of Bioresearch   生物学

    1987.4 - 1992.3

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    Country: Japan

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  • University of Tsukuba   Second Cluster of College   生物学類

    1983.4 - 1987.3

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    Country: Japan

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Professional Memberships

Committee Memberships

  • 日本節足動物発生学会   学会誌編集委員  

    2012.4   

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    Committee type:Academic society

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Papers

  • A microCT-based atlas of the central nervous system and midgut in sea spiders (Pycnogonida) sheds first light on evolutionary trends at the family level Reviewed

    Karina Frankowski, Katsumi Miyazaki, Georg Brenneis

    Frontiers in Zoology   19 ( 1 )   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    Background

    Pycnogonida (sea spiders) is the sister group of all other extant chelicerates (spiders, scorpions and relatives) and thus represents an important taxon to inform early chelicerate evolution. Notably, phylogenetic analyses have challenged traditional hypotheses on the relationships of the major pycnogonid lineages (families), indicating external morphological traits previously used to deduce inter-familial affinities to be highly homoplastic. This erodes some of the support for phylogenetic information content in external morphology and calls for the study of additional data classes to test and underpin in-group relationships advocated in molecular analyses. In this regard, pycnogonid internal anatomy remains largely unexplored and taxon coverage in the studies available is limited.

    Results

    Based on micro-computed X-ray tomography and 3D reconstruction, we created a comprehensive atlas of in-situ representations of the central nervous system and midgut layout in all pycnogonid families. Beyond that, immunolabeling for tubulin and synapsin was used to reveal selected details of ganglionic architecture. The ventral nerve cord consistently features an array of separate ganglia, but some lineages exhibit extended composite ganglia, due to neuromere fusion. Further, inter-ganglionic distances and ganglion positions relative to segment borders vary, with an anterior shift in several families. Intersegmental nerves target longitudinal muscles and are lacking if the latter are reduced. Across families, the midgut displays linear leg diverticula. In Pycnogonidae, however, complex multi-branching diverticula occur, which may be evolutionarily correlated with a reduction of the heart.

    Conclusions

    Several gross neuroanatomical features are linked to external morphology, including intersegmental nerve reduction in concert with trunk segment fusion, or antero-posterior ganglion shifts in partial correlation to trunk elongation/compaction. Mapping on a recent phylogenomic phylogeny shows disjunct distributions of these traits. Other characters show no such dependency and help to underpin closer affinities in sub-branches of the pycnogonid tree, as exemplified by the tripartite subesophageal ganglion of Pycnogonidae and Rhynchothoracidae. Building on this gross anatomical atlas, future studies should now aim to leverage the full potential of neuroanatomy for phylogenetic interrogation by deciphering pycnogonid nervous system architecture in more detail, given that pioneering work on neuron subsets revealed complex character sets with unequivocal homologies across some families.

    DOI: 10.1186/s12983-022-00459-8

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    Other Link: https://link.springer.com/article/10.1186/s12983-022-00459-8/fulltext.html

  • トゲウミグモの生活史を追う:SCUBA 潜水による観察と採集を通して

    宮﨑勝己, 星野修

    JAMBIO News Letter   11   2 - 5   2022.3

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

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  • A taxonomic list of sea spiders (Arthropoda, Pycnogonida) from Japanese waters Reviewed

    Katsumi Miyazaki

    Zoological Science   39 ( 1 )   16 - 40   2022.1

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Zoological Society of Japan  

    DOI: 10.2108/zs210077

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  • Characteristics of the newly discovered cement glands in the sea spider, Nymphonella tapetis (Pycnogonida, Ascorhynchidae).

    Proceedings of the Arthropodan Embryological Society of Japan   54   23   2022

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  • Comparison of postembryonic developmental mode between the populations of Izu and Kii peninsulas of the “cosmopolitan” sea spider, Ammothella biunguiculata (Pycnogonida, Ammotheidae).

    Proceedings of the Arthropodan Embryological Society of Japan   54   21 - 22   2022

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  • ミトコンドリアCOI遺伝子塩基配列に基づく日本産カイヤドリウミグモの集団遺伝学的解析 Reviewed

    五十嵐陽大, 玉置雅紀, 宮﨑勝己

    水生動物   2020 ( 4 )   10 pp.   2020.5

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  • カイヤドリウミグモ研究の軌跡 Reviewed

    宮﨑勝己, 山田勝雅

    生物科学   70 ( 2 )   66 - 72   2019.2

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  • カイヤドリウミグモ研究のゆくえ Reviewed

    山田勝雅, 張成年, 鳥羽光晴, 良永知義, 冨山毅, 望月佑一, 宮﨑勝己

    生物科学   70 ( 2 )   103 - 111   2019.2

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  • カイヤドリウミグモの系統と分類 Reviewed

    玉置雅紀, 宮﨑勝己, 張成年

    生物科学   70 ( 2 )   73 - 77   2019.2

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  • 技法の開発:カイヤドリウミグモの早期発見と基礎生態 Reviewed

    宮﨑勝己, 良永知義, 山下桂司, 中木舞, 神谷享子, 恩地啓実, 山田勝雅, 望月佑一, 玉置雅紀

    生物科学   70 ( 2 )   95 - 102   2019.2

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  • Outline of the life history and postembryonic development in a pycnogonid, Nymphopsis muscosa (Pycnogonida, Ammotheidae) at Izu Oshima Island, Japan. Reviewed

    Katsumi Miyazaki

    Proceedings of the Arthropodan Embryological Society of Japan   52   19 - 21   2019

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  • Impact of sea spider parasitism on host clams: susceptibility and intensity-dependent mortality Reviewed

    Katsumasa Yamada, Katsumi Miyazaki, Takeshi Tomiyama, Gen Kanaya, Yoshifumi Miyama, Tomoyoshi Yoshinaga, Kunihiro Wakui, Masanori Tamaoki, Mitsuharu Toba

    JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM   98 ( 4 )   735 - 742   2018.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CAMBRIDGE UNIV PRESS  

    Nymphonella tapetis (Pycnogonida, Ascorhynchidae) is an endoparasitic sea spider affecting bivalves. Recently, sea spiders have been found on a massive scale in the commercially important Manila clams (Veneridae, Ruditapes philippinarum) in Japan (Tokyo Bay). Simultaneously, mass mortality has occurred in this area. Local fishers assumed that this mass mortality was caused by the parasitic sea spider, despite the effect of the parasite and parasite intensity on the host being unknown. To evaluate the susceptibility of the Manila clam to sea spider infestation and the impact on mortality levels, we established six treatments at different infection intensities (density of newly hatched larvae of sea spiders) over a 6-month long laboratory experiment. We monitored mortality and three susceptibility indices (clearance rate, sand-burrowing speed and adductor muscle strength) under sufficient food conditions. Parasitization by sea spider affected clearance rate and sand-burrowing speed. The pattern of parasitic intensity effects on survival of Manila clam hosts was shown to be dependent on the levels of parasite numbers, i.e. clams with lower parasitic levels (total of <200 hatching larvae of sea spider given to a host) have a higher survival rate, and high mortality of host clams was shown in excessively higher parasitic densities (400-4000 individuals). Such pattern of parasitic effects on host survival might be one of the causes of mass mortality of Manila clams occurring in the field.

    DOI: 10.1017/S0025315417000200

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  • 田辺湾および和歌山県におけるウミグモ類相の調査報告

    前田夏実, 松井淳, 宮﨑勝己

    奈良教育大学自然環境教育センター紀要   ( 18 )   9 - 19   2017

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  • Preliminary notes on the life history of a pycnogonid, Ammothella biunguiculata (Pycnogonida, Ammotheidae): Comparison between the populations of Izu and Kii peninsulas. Reviewed

    Yuichi Mochizuki, Katsumi Miyazaki

    Proceedings of the Arthropodan Embryological Society of Japan   48   59 - 60   2017

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  • Postembryonic development of the sea spider Ammothella biunguiculata (Pycnogonida, Ammotheidae) endoparasitic to an actinian Entacmaea quadricolor (Anthozoa, Stichodactylidae) in Izu Peninsula, Japan Reviewed

    Yuichi Mochizuki, Katsumi Miyazaki

    INVERTEBRATE REPRODUCTION & DEVELOPMENT   61 ( 3 )   189 - 199   2017

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    Postembryonic developmental stages of an endoparasitic pycnogonid, Ammothella biunguiculata in Izu Peninsula, Japan are described. Eleven stages were identified beginning with a protonymphon larva attached to the male oviger. We found endoparasitic individuals in the host actinian from the second to tenth instar, and forms in the ninth stage to adult were found free-living. This indicates a transition from being endoparasitic to free-living during the ninth to tenth instar stages. The first instar protonymphon attached to the adult male oviger has a gland duct on the anterior margin of each chelifore scape which completely disappears with the second instar. The disappearance of the chelifore gland duct coincides with the beginning of an endoparasitic stage in the development of this species. Although the larval morphology and the postembryonic development of pycnogonids have been summarized by several authors, the present study concludes that much remains to be learnt.

    DOI: 10.1080/07924259.2017.1291452

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  • Impact of sea spider parasitism on host clams: relationships between burial patterns and parasite loads, somatic condition and survival of host Reviewed

    Takeshi Tomiyama, Katsumasa Yamada, Kunihiro Wakui, Masanori Tamaoki, Katsumi Miyazaki

    HYDROBIOLOGIA   770 ( 1 )   15 - 26   2016.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    A sudden outbreak of the parasitic sea spider Nymphonella tapetis accompanied by mass mortality of the Manila (asari) clam Ruditapes philippinarum was observed in Japan in 2007. It was unknown why dead and dying clams were found on the sand surface. In our laboratory experiment, in which sea spiders were artificially parasitised to host clams, unburied clams were significantly increased, suggesting that sea spider parasitism can change the burial mode of clams. We compared parasite loads, somatic condition and reburial activity of Manila clams among three burial groups (fully buried, partly buried and unburied) collected from the field. Unburied and partly buried clams had 30% lower soft body weight and 60% lower adductor strength as compared to the fully buried clams. Parasite prevalence and the mean infection intensity were greater in unburied and partly buried clams than in fully buried clams. Reburial depth was smaller in unburied and partly buried clams than in fully buried clams under laboratory conditions. Approximately 30% of the unburied and partly buried clams died during 30 days, whereas fully buried clams suffered no mortality. These results indicate that sea spider parasitism can reduce the burial depth and somatic condition of host clams, and eventually cause clam mortality on the sand.

    DOI: 10.1007/s10750-015-2564-y

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  • Morphology, distribution and mode of life in the sea spider Nymphonella spp. (Arthropoda, Pycnogonida): Potential endoparasitism on Asari outside Japan.

    Katsumi Miyazaki, Takeshi Tomiyama, Katsumasa Yamada, Masanori Tamaoki

    Bulletin of Japan Fisheries Research and Education Agency   ( 42 )   95   2016

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  • Marine benthic community in Shirahama, southwestern Kii Peninsula, central Japan. Reviewed

    Masanori Okanishi, Asuka Sentoku, Shinta Fujimoto, Naoto Jimi, Ryo Nakayama, Yusuke Yamana, Hiroki Yamauchi, Hayate Tanakai, Tetsuya Kato, Sho Kashio, Daisuke Uyeno, Kohki Yamamoto, Katsumi Miyazaki, Akira Asakura

    Publications of the Seto Marine Biological Laboratory   44   7 - 52   2016

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Seto Marine Biological Laboratory, Kyoto University  

    DOI: 10.5134/217458

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  • Dynamics of the sea spider parasitism on Asari in Matsukawaura Lagoon, Japan.

    Takeshi Tomiyama, Takashi Iwasaki, Kunihiro Wakui, Katsumasa Yamada, Masanori Tamaoki, Katsumi Miyazaki

    Bulletin of Japan Fisheries Research and Education Agency   (42) ( 42 )   94   2016

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  • Life history and distribution of Nymphonella tapetis, a sea spider parasitic on the Manila clam, Ruditapes philippinarum in Tokyo Bay, Japan.

    Katsumasa Yamada, Katsumi Miyazaki, Seinen Chow, Toshihiro Yamamoto, Takeshi Tomiyama, Tomoyoshi Yoshinaga, Yoshifumi Miyama, Masanori Tamaoki, Mitsuharu Toba

    Bulletin of Japan Fisheries Research and Education Agency   ( 42 )   94 - 95   2016

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  • 18S ANALYSIS OF THE TAXONOMIC POSITION OF AN ENDOPARASITIC PYCNOGONID, NYMPHONELLA TAPETIS (ARTHROPODA: PYCNOGONIDA: ASCORHYNCHIDAE) Reviewed

    Katsumi Miyazaki, Takeshi Tomiyama, Katsumasa Yamada, Masanori Tamaoki

    JOURNAL OF CRUSTACEAN BIOLOGY   35 ( 4 )   491 - 494   2015.7

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    We obtained the nucleotide sequence of 18S rDNA of Nymphonella tapetis, a pycnogonid endoparasitic on some bivalves, together with that of other species belonging to Ascorhynchidae Hoek, 1881. The phylogenetic tree based on these sequences as well as those of selected species from a database strongly supports the monophyly of Ascorhynchidae and the inclusion of Nymphonella in the family. Nymphonella seems to be an aberrant form in the Ascorhynchidae, but multi-gene analysis with more species is required for the exact determination of the taxonomic position of the genus.

    DOI: 10.1163/1937240X-00002348

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  • Direct evidence of sexual reproduction in the zebra coral, Oulastrea crispata (Anthozoa, Scleractinia), in Japan Reviewed

    Yuna Zayasu, Katsumi Miyazaki, Yi-Ting Lien, Nami Okubo

    INVERTEBRATE REPRODUCTION & DEVELOPMENT   59 ( 2 )   61 - 65   2015.4

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    Reproductive traits of scleractinian corals are important in understanding their ecology and for maintaining coral populations; however, it has been reported for no more than 30% of species. Oulastrea crispata (Lamark, 1816) has the greatest north-south distribution in the Indo-West Pacific. This species is also known as one of the most stress-tolerant corals. Thus, most previous studies on O. crispata have examined its physiological, ecological, morphological, or genetic traits and its symbiotic dinoflagellates relative to its broad distribution and its stress tolerance. However, there have been few studies on its reproduction. In this study, the release of sperm and eggs of O. crispata was observed under laboratory rearing conditions in Shirahama, Kii Peninsula, Japan. This is the first direct evidence of sexual reproduction in this species, and the contribution of sexual reproduction to Oulastrea biology is briefly discussed.

    DOI: 10.1080/07924259.2015.1006340

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  • Phylogenetic position of Loricifera inferred from nearly complete 18S and 28S rRNA gene sequences. Reviewed

    Hiroshi Yamasaki, Shinta Fujimoto, Katsumi Miyazaki

    Zoological Letters   1   18 - (9 pp.)   2015

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  • 京都大学瀬戸臨海実験所で観測された降水の安定同位体比の変動特性.瀬戸臨海実験所年報

    田上雅浩, 一柳錦平, 望月佑一, 宮﨑勝己

    瀬戸臨海実験所年報   ( 28 )   33 - 35   2015

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  • 寄生-宿主間の栄養関係に関する研究のこれまでとこれから:アサリ-カイヤドリウミグモを事例に

    山田勝雅, 金谷弦, 宮崎勝己, 富山毅, 玉置雅紀

    日本水産学会誌   81   888 - 889   2015

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  • 古座川の水質および水生昆虫調査(ポケットゼミ「地域連環学入門」水生昆虫班報告)

    齋藤純, 鈴木英介, 張碧霄, 山田康太, 宮崎勝己, 中野智之

    古座川合同調査報告集   10   431 - 434   2015

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  • マクロベントス採集を目的としたドレッジ装置の作製

    山内洋紀, 山本恒紀, 宮﨑勝己

    瀬戸臨海実験所年報   ( 28 )   30 - 32   2015

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  • 瀬戸臨海実験所の教育と研究における分類学の重要性-時岡 隆先生と臨海実習に注目して-

    岡西政典, 加藤哲哉, 宮崎勝己

    タクサ   ( 36 )   12 - 16   2014

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  • 盤洲のカイヤドリウミグモ

    鳥羽光晴, 小林豊, 石井亮, 張成年, 山本敏博, 丹羽健太郎, 良永知義, 宮崎勝己

    東京湾の漁業と環境   ( 5 )   79   2014

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  • Annotations by Takushi Horita on Tokioka's paper. Reviewed

    Katsumi Miyazaki

    Publications of the Seto Marine Biological Laboratory   42   105 - 107   2014

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  • 二枚貝寄生性種カイヤドリウミグモの分類学的位置について

    宮崎勝己, 冨山毅, 山田勝雅, 玉置雅紀

    日本動物学会大会予稿集   85th   2014

  • カイヤドリウミグモの寄生強度と宿主アサリの生残率と活力の関係:ウミグモ寄生がアサリ資源を減少させたのか?

    山田勝雅, 宮崎勝己, 冨山毅, 金谷弦, 良永知義, 深山義文, 涌井邦浩, 玉置雅紀, 鳥羽光晴

    日本水産学会大会講演要旨集   2014   2014

  • 潜砂状態の異なるアサリのカイヤドリウミグモ寄生状況と宿主の生残

    冨山毅, 山田勝雅, 涌井邦浩, 玉置雅紀, 宮崎勝己

    日本水産学会大会講演要旨集   2014   2014

  • Phylogenetic and taxonomic status of the coral Goniopora stokesi and related species (Scleractinia: Poritidae) in Japan based on molecular and morphological data Reviewed

    Yuko F. Kitano, Masami Obuchi, Daisuke Uyeno, Katsumi Miyazaki, Hironobu Fukami

    ZOOLOGICAL STUDIES   52   2013.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACAD SINICA INST ZOOLOGY  

    Background: Goniopora stokesi is an uncommon species mainly found in tropical and subtropical regions but was also reported in temperate regions of Japan. This species has two unique characteristics. First, it does not typically attach to hard substrates, i.e., it is free-living. Although non-free-living colonies were reported, it is unclear whether their morphologies exhibit intraspecific or interspecific differences. Second, they can asexually form daughter colonies, which are secondary colonies that grow on the surface (coenosac) of a parent colony and subsequently detach. To date, this specific characteristic has not been reported in Japan, and it is not clear whether this species occurs in Japan.
    Results: To clarify the taxonomic status of this species, we investigated its reproductive mechanism in Japan and morphologically and genetically analyzed specimens collected from both subtropical and temperate regions. We found that this species forms daughter colonies in Okinawa, Japan and that free-living colonies in the temperate region, which were formerly recognized as G. stokesi, likely constitute a morphological variation of a separate species. In addition, all non-free-living colonies with G. stokesi-like morphologies were also morphological variations of other species.
    Conclusions: Overall, free-living colonies with large, deep calices, thin walls, and well-developed epitheca on the underside were G. stokesi, whereas other free-living and all non-free-living G. stokesi-like colonies belonged to other species, such as Goniopora djiboutiensis.

    DOI: 10.1186/1810-522X-52-25

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  • A new marine tardigrade, Tanarctus diplocerus (Arthrotardigrada: Halechiniscidae) from Japan Reviewed

    Shinta Fujimoto, Katsumi Miyazaki, Atsushi C. Suzuki

    JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM   93 ( 4 )   955 - 961   2013.6

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    A new marine tardigrade of the genus Tanarctus is described from Japan. Tanarctus diplocerus sp. nov. was collected from Shimabara Bay, Kumamoto and Tanabe Bay, Wakayama. It is characterized by simple leg IV appendages, the lack of the internal-external relation in the dorsal and ventral cirri and the lack of the club-shaped secondary clavae. The secondary sexual dimorphism of the primary clavae and probable two-digit juveniles of the new species are reported. The problematic terminology of the so-called 'internal' and 'external' cirri is also discussed. A taxonomic key for the species of Tanarctus is provided.

    DOI: 10.1017/S0025315412000689

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  • Neostygarctus lovedeluxe n. sp from the Miyako Islands, Japan: The First Record of Neostygarctidae (Heterotardigrada: Arthrotardigrada) from the Pacific Reviewed

    Shinta Fujimoto, Katsumi Miyazaki

    ZOOLOGICAL SCIENCE   30 ( 5 )   414 - 419   2013.5

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    A new species of the previously monospecific marine tardigrade family Neostygarctidae is described. Neostygarctus lovedeluxe n. sp. was found from a submarine cave in Miyako Islands, Japan. This is the first record of Neostygarctidae from the Pacific. The new species is easily distinguished from the previously known N. acanthophorus by its number of dorsal spines, as N. lovedeluxe has two spines each on the three dorsal body plates in contrast to one in N. acanthophorus. Furthermore the morphology of the two clawed juvenile is reported for the first time in Neostygarctidae, providing new insights into the common sequence in some ontogenic traits.

    DOI: 10.2108/zsj.30.414

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  • Phylogenetic and taxonomic status of the coral goniopora stokesi and related species (Scleractinia: Poritidae) in Japan based on molecular and morphological data Reviewed

    Yuko F. Kitano, Masami Obuchi, Daisuke Uyeno, Katsumi Miyazaki, Hironobu Fukami

    Zoological Studies   52 ( 1 )   25 - (16 pp.)   2013

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Academia Sinica  

    Background: Goniopora stokesi is an uncommon species mainly found in tropical and subtropical regions but was also reported in temperate regions of Japan. This species has two unique characteristics. First, it does not typically attach to hard substrates, i.e., it is free-living. Although non-free-living colonies were reported, it is unclear whether their morphologies exhibit intraspecific or interspecific differences. Second, they can asexually form daughter colonies, which are secondary colonies that grow on the surface (coenosac) of a parent colony and subsequently detach. To date, this specific characteristic has not been reported in Japan, and it is not clear whether this species occurs in Japan. Results: To clarify the taxonomic status of this species, we investigated its reproductive mechanism in Japan and morphologically and genetically analyzed specimens collected from both subtropical and temperate regions. We found that this species forms daughter colonies in Okinawa, Japan and that free-living colonies in the temperate region, which were formerly recognized as G. stokesi, likely constitute a morphological variation of a separate species. In addition, all non-free-living colonies with G. stokesi-like morphologies were also morphological variations of other species. Conclusions: Overall, free-living colonies with large, deep calices, thin walls, and well-developed epitheca on the underside were G. stokesi, whereas other free-living and all non-free-living G. stokesi-like colonies belonged to other species, such as Goniopora djiboutiensis. © 2013 Kitano et al.
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    DOI: 10.1186/1810-522X-52-25

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  • 時岡先生ご遺稿発見と出版までの経緯について

    宮崎勝己

    瀬戸臨海実験所年報   ( 26 )   32 - 33   2013

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  • 仁淀川流域の川と海のクマムシ相の比較研究

    藤本心太, 中野智之, 宮崎勝己

    「森里海連環学による地域循環木文化社会創出事業(木文化プロジェクト)」2012年度報告書   54 - 55   2013

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  • Postembryonic development of the female reproductive system in the pycnogonid Propallene longiceps (Pycnogonida, Callipallenidae) Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    INVERTEBRATE REPRODUCTION & DEVELOPMENT   56 ( 4 )   287 - 292   2012.12

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    The postembryonic development of the female reproductive system in the pycnogonid Propallene longiceps is examined. The germ cells can be detected first in the later stage of the 3rd instar and become a paired gonad covered with gonadal epithelium in the next instar. The larval gonad changes its shape: paired at the 4th instar, reversed U-shaped at the fifth, unpaired at the sixth, and paired again at the seventh. Oocytes can be distinguished, and the extension of the ovary into the walking legs begins at the 7th instar. Growing oocytes protrude outward from the ovary on cellular stalks in the pedal part. The trunk ovary becomes U-shaped, and the oviducts and genital pores start forming at the 8th instar. The disappearance of trunk ovary begins at the 9th instar, and is complete at the next adult stage. The connection between the pedal ovarian lumen and the genital pores via the oviducts is complete in the adult, and the female reproductive system becomes segmentally arranged. This study confirms that the segmental arrangement of adult female reproductive system in P. longiceps, which is unique among recently described arthropods, is a secondary state in pycnogonids attained by reducing the trunk part of ovary.

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  • 古座川の水生昆虫相(2011年度森里海連環学実習B報告)

    福島健太, 藤巻真由, 藤井温子, 宮崎勝己

    古座川合同調査報告集   7   16 - 18   2012

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  • 水生昆虫相及び水質からみた古座川と田原川の比較

    布施翔大, 宮崎勝己

    古座川合同調査報告集   7   13 - 15   2012

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  • Preliminary notes on the freshwater tardigrades from the Kamo River, Kyoto. Reviewed

    Shinta Fujimoto, Katsumi Miyazaki

    Proceedings of the Arthropodan Embryological Society of Japan   46   39 - 40   2011

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  • On the species validity of Nymphonella lecalvezi (Pycnogonida, Ascorhynchidae): A re-evaluation of some morphological characteristics. Reviewed

    Katsumi Miyazaki

    Proceedings of the Arthropodan Embryological Society of Japan   46   35 - 37   2011

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  • Dwarf Males of Octolasmis warwickii (Cirripedia: Thoracica): The First Example of Coexistence of Males and Hermaphrodites in the Suborder Lepadomorpha Reviewed

    Yoichi Yusa, Mayuko Takemura, Katsumi Miyazaki, Tetsuya Watanabe, Shigeyuki Yamato

    BIOLOGICAL BULLETIN   218 ( 3 )   259 - 265   2010.6

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    In the lepadomorph barnacle Octolasmis warwickii, individuals are often found attached to the scutum of conspecifics living externally on the crab hosts. To test whether these conspecific-attached individuals are dwarf males, as are known to occur in other suborders of barnacles, we compared the pattern of attachment, size-frequency distribution, and reproductive status of the conspecific-attached (Con-A) and crab-attached (Crab-A) individuals. Con-As were smaller than Crab-As. There was a positive relationship between the body size of Crab-As and the number of individuals on them. Con-As had longer penises than Crab-As of the same body size, and their testes were better developed. The four largest Con-As examined were brooding eggs. These results indicate that Con-As of O. warwickii are dwarf males, with a potential to become hermaphroditic. This represents the first known example of coexistence of males and hermaphrodites in the suborder Lepadomorpha. The mating group size of O. warwickii was smaller than in its hermaphroditic congeners but larger than in barnacles with dwarf males and females, which supports the current theories that group size is important for the evolution of sexuality patterns in barnacles.

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  • Structure of the adult female reproductive system in the sea spider, Propallene longiceps (Pycnogonida, Callipallenidae) and its functional and evolutionary significance Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    INVERTEBRATE REPRODUCTION & DEVELOPMENT   54 ( 1 )   35 - 39   2010.5

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    The structure of the adult female reproductive system in the pycnogonid. Propallene longiceps, is examined. The ovary is present in each walking leg from the second coxa to femur, containing oocytes in various developmental stages. Oocytes are protruded outward from the ovary on cellular stalks. Each ovary is directly connected with the genital pore through the oviduct in the second coxa. Thus ovulation of the mature ova is into the ovarian lumen and oviposition is through the oviduct and the genital pore. Such a route of ovulation and oviposition has been commonly described in other pycnogonids as well as in euchelicerates. However, the segmental arrangement of the female reproductive system revealed in the present study, with isolation of a complete set of ovary, oviduct and genital pore in each walking leg, is distinct from the situation described in other pycnogonids, and recalls a hypothesized ancestral state in arthropods.

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  • 古座川における水生昆虫調査報告

    拝野晃希, 小川直記, 梶野泰弘, 平林凌太朗, 宮崎勝己, 深見裕伸

    古座川合同調査報告集   4   101 - 106   2010

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  • 2008年度森里海連環学実習Bにおける水生昆虫調査報告

    渡辺順也, 沼田篤志, 宮崎勝己

    古座川合同調査報告集   4   84 - 87   2010

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  • アサリに内部寄生し漁業被害を与えるカイヤドリウミグモの生物学 Reviewed

    宮崎勝己, 小林豊, 鳥羽光晴, 土屋仁

    タクサ   ( 28 )   45 - 54   2010

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  • Early development of the Japanese spiny oyster (Saccostrea kegaki): Characterization of some genetic markers Reviewed

    Shota Kakoi, Koryu Kin, Katsumi Miyazaki, Hiroshi Wada

    ZOOLOGICAL SCIENCE   25 ( 5 )   455 - 464   2008.5

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    The phylum Mollusca is one of the major groups of Lophotrochozoa. Although mollusks exhibit great morphological diversity, only a few comparative embryological studies have been performed on this group. In the present study, to begin understanding the molecular development of the diverse morphology among mollusks, we observed early embryogenesis in a bivalve, the Japanese spiny oyster, Saccostrea kegaki. Although several studies have begun to reveal the genetic machinery for early development in gastropods, very little molecular information is available on bivalve embryogenesis. Thus, as a step toward identifying tissue-specific gene markers, we sequenced about 100 cDNA clones picked randomly from a gastrula-stage cDNA library. This basic information on bivalve embryology will be useful for further studies on the development and evolution of mollusks.

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  • Formation of an egg envelope in the pycnogonid, Propallene longiceps (Pycnogonida, Callipallenidae) Reviewed

    Szczepan M. Bilinski, Beata Szymanska, Katsumi Miyazaki

    ARTHROPOD STRUCTURE & DEVELOPMENT   37 ( 2 )   155 - 162   2008.3

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    Vitellogenic oocytes of all pycnogonids studied so far contain dilated elements of the endoplasmic reticulum, filled with characteristic electron-dense bodies. During vitellogenesis these bodies fuse and form larger, almost spherical granules that were traditionally interpreted as nascent yolk granules. Here, we present the results of ultrastructural investigations of previtellogenic and early vitellogenic oocytes of Propallene longiceps (Pycnogonida, Callipallenidae). We show that the intra-cisternal bodies/granules of pycnogonids are not involved in vitellogenesis but Contain macromolecules that are released from the oocyte and contribute to the formation of an egg envelope. The obtained results are discussed in a phylogenetic context. We suggest that the presence of the intra-cisternal electron-dense bodies in the oocyte cytoplasm represents a plesiomorphic character of arthropods inherited from the arthropod ancestor. (C) 2007 Elsevier Ltd. All rights reserved.

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  • 書評「シリーズ21世紀の動物科学 第2巻 動物の多様性」

    宮﨑勝己

    生物科学ニュース   ( 443 )   7 - 8   2008

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  • Ultrastructural investigations of the ovary and oogenesis in the pycnogonids Cilunculus armatus and Ammothella biunguiculata (Pycnogonida, Ammotheidae) Reviewed

    Katsumi Miyazaki, Szczepan M. Biliński

    Invertebrate Biology   125 ( 4 )   346 - 353   2006.11

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    Ovarian ultrastructure and oogenesis in two pycnogonid species, Cilunculus armatus and Ammothella blunguiculata, were investigated. The ovary is morphologically and functionally divided into trunk and pedal parts. The former represents the germarium and contains very young germ cells in a pachytene or postpachytene phase, whereas the latter houses developing previtellogenic and vitellogenic oocytes and represents the vitellarium. Intercellular bridges were occasionally found between young (trunk) germ cells. This indicates that in pycnogonids, as in other animal groups, at the onset of oogenesis clusters of germ cells are generated. As nurse cells are absent in the ovaries of investigated species, the clusters must secondarily split into individual oocytes. In the vitellarium, the oocytes are located outside the ovary. Each oocyte is connected to the ovarian tissue by a stalk composed of several somatic cells. The stalk cells directly associated with the oocyte are equipped with irregular projections that reach the oocyte plasma membrane. This observation suggests that the stalk cells may play a nutritive role. The ooplasm of vitellogenic oocytes comprises mitochondria, free ribosomes, stacks of annulate lamellae, active Golgi complexes, and vesicles derived from these complexes. Within the latter, numerous electron-dense bodies are present. We suggest that these bodies contribute to yolk formation. © 2006, The American Microscopical Society, Inc.

    DOI: 10.1111/j.1744-7410.2006.00066.x

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  • クモのようでクモでない-ウミグモ類のかたちと生きざまの不思議

    宮崎勝己

    遺伝   59   9 - 12   2005

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  • 海域ステーション 瀬戸臨海実験所

    宮﨑勝己

    FSERC News   ( 4 )   3 - 4   2005

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  • フタツメイソウミグモAmmothella biunguiculataの循環器官系:その形態と機能 Reviewed

    宮崎勝己, Günther Pass

    Proceedings of the Arthropodan Embryological Society of Japan   39   53 - 54   2004

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  • 文科省SPP事業による尼崎小田高校臨海実習(於瀬戸臨海実験所)実施報告

    宮﨑勝己

    FSERC News   ( 2 )   2 - 3   2004

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  • On the position of genital pores in a sea spider, Austrodecus tubiferum (Pycnogonida, Austrodecidae). Reviewed

    Katsumi Miyazaki

    Publications of the Seto Marine Biological Laboratory   40   107 - 111   2004

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  • An ascidian RING finger gene is specifically expressed in a single cell of larval ocellus Reviewed

    XT Sun, M Okuyama, K Miyazaki, SC Zhang, H Wada

    GENE   312   111 - 116   2003.7

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    The ascidian nervous system is extremely simple, although the structure of it is comparable with the complex vertebrate nervous system. This simplicity makes the ascidian nervous system a good model to understand how the neuronal circuit is built up in the chordate nervous system. In order to study the formation of the neuronal circuit at the single cell level, molecular markers to characterize specific single cells are desired. In the present paper, we describe the gene expression pattern of CiGl: an ascidian homologue of Goliath, a Drosophila RING-finger gene. In the early embryonic stage, CiGl is expressed in the lateral part of the neural tube and in several peripheral nerve cells. Later in the larval stage, CiGl specifically marks ocellus: one of the pigment cells in the ascidian brain, which is involved in the photoreceptive system. CiGl will be useful to understand the differentiation mechanism of ocellus, and especially to test the model proposed by Darras and Nishida (2001). In addition, the finding of this single cells specific gene expression pattern at a certain developmental stage encourages us to look for more genes which mark single cells, especially those that have not been well characterized. (C) 2003 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-1119(03)00606-1

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  • On the shape of foregut lumen in sea spiders (Arthropoda : Pycnogonida) Reviewed

    K Miyazaki

    JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM   82 ( 6 )   1037 - 1038   2002.12

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    The shape of foregut lumen in both the pharyngeal and the oesophageal regions has been examined from serial histological sections in 16 species of pycnogonids covering all extant families. Each species has a Y-shaped foregut lumen in transverse section. This result shows that some previous authors misoriented the lumen as inverted Y-shaped. The triradiate Y-shaped foregut lumen has been found among ecdysozoans including tardigrades, onychophorans and scatteringly arthropods. At present, it is not clear whether the Y-shaped state of the characteristic in pycnogonids is convergent or symplesiomorphy with that in tardigrades and onychophorans.

    DOI: 10.1017/S0025315402006628

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  • サンゴイソギンチャクに寄生するフタツメイソウミグモの生活史

    宮崎勝己

    うみうし通信   ( 37 )   6 - 9   2002

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  • Occurrence of juvenile forms of a pycnogonid, Ammothella biunguiculata (Pycnogonida, Ammotheidae) in an actinian, Entacmaea actinostoloides (Anthozoa, Stichodactylidae). Reviewed

    Katsumi Miyazaki

    Proceedings of the Arthropodan Embryological Society of Japan   37   43 - 44   2002

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  • Histological and scanning electron microscopic study on the branchial crown of Hydroides elegans (Haswell, 1883) (Polychaeta, Serpulidae) with consideration of its functional and taxonomical value.

    Mohamed H. Mona, Katsumi Miyazaki, Amal E. Atlam

    Proceedings of the International Conference on Biological Sciences   2   128 - 145   2002

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  • Structure of the female reproductive system and oogenetic mode in a schizomid, Schizomus sawadai (Arachnida, Schizomida) Reviewed

    K Miyazaki, R Ueshima, T Makioka

    INVERTEBRATE REPRODUCTION & DEVELOPMENT   40 ( 1 )   1 - 7   2001.12

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    The structure of the adult female reproductive system was examined in a schizomid, Schizomus sawadai. The system consists of a single median ovary, a pair of lateral oviducts, and 4 single common oviduct with a pair of triplets of small seminal receptacles and a simple slit-like genital pore. Smaller previtellogenic oocytes less than 40 gm in diameter are found sparsely scattered in the dorsal ovarian wall among the ovarian epithelial cells, while two size groups of advanced oocytes, larger previtellogenic and vitellogenic, protrude from the lateral and ventral ovarian walls, respectively, accompanied by short cellular stalks. Neither nurse cells nor follicle cells are present around all the oocytes. Oogonia are not found in the adult ovary, indicating the lack of a continued supply of oocytes. The ovarian lumen is connected with the lumens of the lateral and common oviducts, and then with the genital pore. Modes of oogenesis, ovulation, and oviposition, deduced from the structural features of the female reproductive system of S. sawadai, are compared with those in some other arachnids, merostomes and pycnogonids.

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  • European Colloquium of Arachnology 参加記(2)

    宮崎勝己

    遊絲   ( 6 )   8 - 11   2000

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  • Comparative morphology of the circulatory system in pycnogonids (Arthropoda, Pycnogonida).

    Katsumi Miyazaki

    Book of Abstracts, XVIIIth International Congress of Zoology   194 - 195   2000

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  • European Colloquium of Arachnology 参加記(1)

    宮崎勝己

    遊絲   ( 5 )   13 - 15   1999

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  • Structure of the female reproductive system and oogenesis in pycnogonids (Chelicerata, Pycnogonida); Comparison with other chelicerates and arthropods.

    Katsumi Miyazaki, S.M. Biliński, Toshiki Makioka

    Abstracts 18th European Colloquium of Arachnology   39   1999

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  • Homology of the "Dohrn's septum" in pycnogonids. Reviewed

    Katsumi Miyazaki

    Proceedings of the Arthropodan Embryological Society of Japan   33   15 - 16   1998

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  • Transgenic medaka fish bearing the mouse tyrosinase gene: Expression and transmission of the transgene following electroporation of the orange-colored variant Reviewed

    H Ono, E Hirose, K Miyazaki, H Yamamoto, J Matsumoto

    PIGMENT CELL RESEARCH   10 ( 3 )   168 - 175   1997.6

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    Transgenic fish bearing the mouse tyrosinase gene (mg-Tyrs-J) were produced by transfection into fertilized eggs of the homozygous normal orange-colored variant of medaka fish, Oryzias latipes, by means of electroporation. Of 589 eggs transfected, 38 fish (6%) exhibited brownish wild-type skin pigmentation, which was discernible from control siblings. Light microscopy of the skin from the founders thus generated disclosed that 1) melanization occurred and was restricted to melanophores formed presumably from preexisting amelanotic melanophores, 2) there was a wide variation in the degree of melanization observed among melanophores, and 3) no melanin deposition was recognized in xanthophores or leucophores. Immunofluorescence using an antibody raised against mouse tyrosinase disclosed that melanophores at varying stages of maturation were reactive. Thus, it was shown that the transgene in medaka fish expressed its action in a cell type-specific manner. Crossing of transgenic founders with homozygous orange-colored variant fish yielded two groups of offspring expressing either the wild-type or the orange-colored skin pigmentation at an approximate ratio of 1:1. Crossing between founders exhibiting wild-type pigmentation yielded only offspring with melanized skin. Skin melanophores in these offspring formed vertical stripes, which are rare in this species. The hereditary basis of melanized skin was demonstrated in matings of F1 progenies, which resulted in similar degrees of melanization over whole skin melanophores. The sum of these findings implied that the transgene is expressed as a dominant character gene and is transmitted through germ cell lines according to the Mendelian law. PCR analysis combined with nested PCR technique strongly suggested that the transgene was integrated into the medaka genome, even though the copy number deduced from gel banding was largely diminished, possibly as a result of fragmentation or instability within the medaka genome.

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  • フタツメイソウミグモAmmothella biunguiculataプロトニムフォン幼生の外部形態 Reviewed

    宮崎勝己, 鈴木浩文

    Proceedings of the Arthropodan Embryological Society of Japan   32   29 - 31   1997

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  • Shallow-water Pycnogonida from Ogasawara (Bonin) Islands with a description of Achelia curticauda n. sp. Reviewed

    Koichiro Nakamura, Katsumi Miyazaki, C. Allan Child

    Species Diversity   1   117 - 121   1996

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    DOI: 10.12782/specdiv.1.117

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  • Scanning electron microscopic observations on the so-called "male genital pore" in the pycnogonid, Rhynchothorax mediterraneus (Pycnogonida: Rhynchothoracidae). Reviewed

    Katsumi Miyazaki

    Proceedings of the Arthropodan Embryological Society of Japan   31   1 - 3   1996

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  • Structure of the adult male reproductive system in a pycnogonid, Cilunculus armatus (Pycnogonida: Ammotheidae). Reviewed

    Katsumi Miyazaki

    Publications of the Seto Marine Biological Laboratory   37   329 - 335   1996

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  • Preliminary observations on the foot in the homing limpets, Siphonaria acmaeoides (Pilsbry) and Patelloida saccharina lanx (Reeve) with light and scanning electron microscopy. Reviewed

    Mohamed H. Mona, Katsumi Miyazaki

    Publications of the Seto Marine Biological Laboratory   36 ( 5 )   313 - 324   1995

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    DOI: 10.5134/176249

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  • Rhynchothorax mediterraneus, the first record of Rhynchothoracidae (Pycnogonida) from Japan. Reviewed

    Katsumi Miyazaki, Jan, H. Stock

    Publications of the Seto Marine Biological Laboratory   36   325 - 327   1995

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  • カエル類精子の走査型電子顕微鏡・光学顕微鏡試料作成法

    宮崎勝己, 福山欣司

    Hiyoshi Review of Natural Science, Keio University   15   67 - 71   1994

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  • Notes on the female genital pores in extra-legged species of pycnogonids (Arthropoda; Pycnogonida), Pentacolossendeis reticulata (Colossendeidae) and Pentapycnon geayi (Pycnogonidae).

    Katsumi Miyazaki, Toshiki Makioka

    Hiyoshi Review of Natural Science, Keio University   16 ( 16 )   21 - 24   1994

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  • Notes on the route of ovulation in a whip-scorpion, Typopeltis crucifer (Arachnida, Thelyphonida). Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    Proceedings of the Arthropodan Embryological Society of Japan   29   7 - 8   1994

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  • A CASE OF INTERSEXUALITY IN THE SEA SPIDER, CILUNCULUS-ARMATUS (PYCNOGONIDA, AMMOTHEIDAE) Reviewed

    K MIYAZAKI, T MAKIOKA

    ZOOLOGICAL SCIENCE   10 ( 1 )   127 - 132   1993.2

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    An intersexual specimen of the sea spider, Cilunculus armatus, having female and male characteristics together with some intermediate ones was collected from Sagami Bay. Eggs are seen through the cuticle of each femoral segment of walking legs. At the same time, cement gland, peculiar to male, is also found on the same segment of each walking leg, but somewhat atrophied and distally shifted. Genital pore opening on the second coxal segment of each walking leg as seen in female is of intermediate size between the sizes of both sexes. Oviger is intermediate in size and shape, and the number and distributional pattern of ovigerous compound spines are also intermediate.

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  • Functional and phylogenetic significance of pycnogonid oviduct.

    Katsumi Miyazaki, Toshiki Makioka

    Hiyoshi Review of Natural Science, Keio University   13   1 - 6   1993

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  • Application of SEM to the gonad embedded in paraffin in a pycnogonid, Ascorhynchus ramipes (Pycnogonida; Ammotheidae) and an ascidian, Herdmania momus (Ascidiacea; Pyuridae).

    Katsumi Miyazaki, Euichi Hirose

    Hiyoshi Review of Natural Science, Keio University   14   18 - 21   1993

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  • Structure of the female reproductive system and oogenesis in sea spiders (Arthropoda; Pycnogonida)

    Katsumi Miyazaki

    1992.3

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  • Adult female reproductive system and oogenesis in the sea spider, Pycnogonum litorale (Pycnogonida; Pycnogonidae). Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    Zoologische Jahrbücher, Abteilung für Anatomie und Ontogenie der Tiere   122   55 - 56   1992

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  • Preliminary notes on the ultrastructure of the ovary in Cilunculus armatus (Pycnogonida, Ammotheidae). Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    Proceedings of the Arthropodan Embryological Society of Japan   27   5 - 7   1992

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  • Preliminary note on the postembryonic development of ovary in Propallene longiceps (Pycnogonida, Callipallenidae). Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    Proceedings of the Arthropodan Embryological Society of Japan   26   1 - 2   1991

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  • Structure of the adult female reproductive system and oogenetic mode in the sea spider, Endeis nodosa (Pycnogonida; Endeidae) Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    Journal of Morphology   209 ( 3 )   257 - 263   1991

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    As in other pycnogonids, the adult female reproductive system of Endeis nodosa is limited to the trunk and walking legs of the cephalothorax. The U‐shaped trunk ovary extends eight blind branches separately into the corresponding walking legs up to each femoral segment. In both the trunk and pedal region, the ovary lies dorsally above the gut, sandwiched between the dorsal and the ventral layer of the horizontal septum. Oogonia and very young oocytes occur throughout the ovary. They fill the trunk ovary and, in the tubular pedal ovary, are aggregated into several cord‐shaped longitudinally arranged germ zones. More advanced oocytes occur only in the pedal ovary. None lie in the germ zones. but instead they protrude individually from the wall of the pedal ovary into the hemocoel on cellular stalks. Vitellogenesis occurs in the larger stalked oocytes. An oval genital pore with a cuticular lid lies on the ventral surface of the second coxa of each walking leg. The pedal ovarian lumen is directly connected to the genital pore by a short oviduct in the second coxa. The ovarian structure and oogenetic modes in this pycnogonid species show some common characteristics with those of most chelicerates. Copyright © 1991 Wiley‐Liss, Inc.

    DOI: 10.1002/jmor.1052090303

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  • Ovarian structure and oogenesis in pycnogonids: Some similarities to those in chelicerates. Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    Proceedings of the Arthropodan Embryological Society of Japan   25   1 - 3   1990

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  • The egg-capsules of ectoparasitic marine triclads collected from the four extant species of horseshoe crabs.

    Masaharu Kawakatsu, Kouichi Sekiguchi, Katsumi Miyazaki, Toshiki Makioka

    Bulletin of the Department of Management and Infornation Sciences, Jobu University   1   15 - 29   1989

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  • Chelicerate-like oogenesis in Endeis nodosa (Pycnogonida; Endeidae). Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    Proceedings of the Arthropodan Embryological Society of Japan   24   11 - 12   1989

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  • Observations on the ovarian structure and oogenesis of some pycnogonids. Reviewed

    Katsumi Miyazaki, Toshiki Makioka

    Proceedings of the Arthropodan Embryological Society of Japan   23   15 - 16   1988

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Books

  • 海産無脊椎動物多様性学-100年の歴史とフロンティア

    宮﨑勝己( Role: Contributor ,  大量発生し漁業被害を与える海産節足動物カイヤドリウミグモをめぐる様々の謎)

    京都大学学術出版会  2022.11  ( ISBN:9784814004492

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    Total pages:706   Responsible for pages:607-617  

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  • カイヤドリウミグモ:大発生からの研究の動向

    宮﨑 勝己( Role: Edit ,  生物科学第70巻第2号)

    農山漁村文化協会  2019.2 

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  • 進化学事典

    宮﨑勝己( Role: Contributor ,  節足動物)

    共立出版  2012.4  ( ISBN:9784320057777

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    Total pages:996   Responsible for pages:268-270   Language:Japanese Book type:Dictionary, encyclopedia

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  • バイオディバーシティシリーズ6,節足動物の多様性と進化

    宮崎勝己( Role: Contributor ,  節足動物門の概説)

    裳華房  2008.4  ( ISBN:9784785358297

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    Total pages:516   Responsible for pages:110-113   Language:Japanese Book type:Scholarly book

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  • バイオディバーシティシリーズ6,節足動物の多様性と進化

    宮崎勝己( Role: Contributor ,  節足動物における分類学の歴史)

    裳華房  2008.4  ( ISBN:9784785358297

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    Total pages:516   Responsible for pages:2-10   Language:Japanese Book type:Scholarly book

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  • バイオディバーシティシリーズ6,節足動物の多様性と進化

    宮崎勝己( Role: Contributor ,  節足動物全体の分類体系・系統の現状)

    裳華房  2008.4  ( ISBN:9784785358297

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    Total pages:516   Responsible for pages:11-27   Language:Japanese Book type:Scholarly book

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  • 森と里と海のつながり 京大フィールド研の挑戦

    宮崎勝己, 大和茂之( Role: Contributor ,  海の動物の多様性について)

    大伸社  2004.7  ( ISBN:4777901424

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    Total pages:151   Responsible for pages:16-21   Language:Japanese Book type:Scholarly book

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  • European Arachnology 2002

    Katsumi Miyazaki, Günther Pass( Role: Contributor ,  Morphology of the circulatory system in a sea spider, Ammothella biunguiculata (Pycnogonida, Ammotheidae), with special reference to the cephalic region.)

    Plant Protection Institute & Berzsenyi Dániel College  2004  ( ISBN:963214791X

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    Total pages:356   Responsible for pages:8992   Language:English Book type:Scholarly book

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MISC

  • カイヤドリウミグモの後胚発生段階区分とその現場動態調査への応用

    宮崎勝己, 山田勝雅, 鳥羽光晴

    日本水産学会大会講演要旨集   2013   2013

  • カイヤドリウミグモの宿主利用パターン:寄生されやすい宿主の特徴とは?

    山田勝雅, 宮崎勝己, 張成年, 山本敏博, 丹羽健太郎, 良永知義, 山北剛久, 熊谷直喜, 深山義文, 小林豊, 鳥羽光晴

    日本ベントス学会・日本プランクトン学会合同大会講演要旨集   2011   2011

  • 外来種?在来種?東京湾に大発生した二枚貝に寄生する小型節足動物:カイヤドリウミグモ

    山田勝雅, 小林豊, 深山義文, 張成年, 山本敏博, 柴田玲奈, 宮崎勝己, 山北剛久, 鳥羽光晴

    日本生態学会大会講演要旨集   58th   2011

Research Projects

  • カイヤドリウミグモ寄生によるアサリ漁業被害に関する研究:効果的対策手法の提案

    Grant number:20H03062

    2020.4 - 2024.3

    System name:科学研究費助成事業 基盤研究(B)

    Research category:基盤研究(B)

    Awarding organization:日本学術振興会

    宮崎 勝己, 玉置 雅紀, 冨山 毅, 山田 勝雅

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    Authorship:Principal investigator 

    Grant amount:\17680000 ( Direct Cost: \13600000 、 Indirect Cost:\4080000 )

    ミトコンドリアDNA COI領域を用いた、カイヤドリウミグモ各個体群間の集団遺伝学的解析を行い、東京湾個体群が地理的距離の近い館山・三崎個体群よりも、三河湾及び松川浦個体群と遺伝的距離がごく近く、東京湾・三河湾・松川浦個体群と館山・三崎個体群が異なる系統群を形成することを明らかにした。また前者がボトルネック効果を受けており、小規模の同祖個体群から派生してきたことが強く示唆された。マイクロサテライトマーカーを用いた予備解析においても、解析数が少ないながら、同様の傾向を見出した。
    環境DNA中からカイヤドリウミグモのDNAを特異的に増幅させるためのプライマーセットの探索を行い、ミトコンドリアD-loop中に特異的なプライマーを構築した。
    過去に東京湾で行った野外調査のデータを整理し、シオフキにおいてもアサリ同様に寄生率が高いことと、バカガイでは寄生がみられなかったことを確認した。また野外調査で得られたカイヤドリウミグモ体長データのコホート解析を行い、個体群サイズに変動をもたらすパラメーター(成長・生残等)の抽出を行った。

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  • 新潟沖日本海産キタクシノハクモヒトデの成長と生活史に関する研究

    2019.4 - 2020.3

    System name:試験研究費助成

    Awarding organization:内田エネルギー科学振興財団

    宮﨑勝己

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  • 新潟沖日本海産深海性クモヒトデ類の生活史に関する研究

    2017.4 - 2018.3

    System name:試験研究費助成

    Awarding organization:内田エネルギー科学振興財団

    宮﨑勝己

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  • 三浦半島における浅海性ウミグモ相に関する研究

    2015.4 - 2016.3

    System name:共同利用・共同研究

    Awarding organization:マリンバイオ共同推進機構(JAMBIO)

    宮﨑勝己

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  • Studies on the parasitic ecology and evolution of Nymphonella tapetis, a harmful pycnogonid endoparasitic on the commercial bivalve, Ruditapes philippinarum.

    Grant number:26292105

    2014.4 - 2017.3

    System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    Miyazaki Katsumi, TOBA Mitsuharu, YOSHINAGA Tomoyoshi, WAKUI Kunihiro, MIYAMA Yoshifumi, KANAYA Gen, Tomás Munilla León

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    Grant amount:\10660000 ( Direct Cost: \8200000 、 Indirect Cost:\2460000 )

    18S rDNA analysis revealed the taxonomic position of Nymphonella tapetis within a monophyletic family Ascorhynchidae. Population genetic analysis using 19 newly developed microsatellite markers showed that the N. tapetis populations of Tokyo bay and Mikawa bay shared the same origin, whereas the population of Misaki, Moura peninsula was an independent one.
    Vanishment of N. tapetis in Matsukawaura, Fukushima during 2014 was confirmed. Field observations revealed that the N. tapetis parasitism can reduce the burial depth and somatic condition of host clams, and eventually cause clam mortality on the sand. Laboratory experiments suggested that mass mortality of host clams was possibly caused by interactive effects between the parasitic infection and other environmental factors.

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  • 下田・狼煙崎サンゴイソギンチャク群落に寄生するフタツメイソウミグモの生活史に関する研究(継続)

    2013.4 - 2014.3

    System name:共同利用・共同研究

    Awarding organization:マリンバイオ共同推進機構(JAMBIO)

    宮﨑勝己

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  • 下田・狼煙崎サンゴイソギンチャク群落に寄生するフタツメイソウミグモの生活史に関する研究

    2012.4 - 2013.3

    System name:共同利用・共同研究

    Awarding organization:マリンバイオ共同推進機構(JAMBIO)

    宮﨑勝己

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  • 海岸性ウミグモ及びクマムシ相-伊豆半島と紀伊半島の比較

    2011.4 - 2012.3

    System name:共同利用・共同研究

    Awarding organization:マリンバイオ共同推進機構(JAMBIO)

    宮﨑勝己;藤本心太

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  • カイヤドリウミグモの寄生被害を回避軽減するためのアサリ放流生産手法の開発

    2010.4 - 2013.3

    System name:新たな農林水産政策を推進する実用技術開発事業

    Awarding organization:農林水産省

    鳥羽光晴, 宮﨑勝己他

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    Authorship:Coinvestigator(s) 

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  • 伊豆半島の海産クマムシ相の解明-実験好適種の発見を目指して

    2010.4 - 2011.3

    System name:共同利用・共同研究

    Awarding organization:マリンバイオ共同推進機構(JAMBIO)

    宮﨑勝己, 藤本心太

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  • ウミグモ対策漁場改善手法開発

    2008.4 - 2010.3

    System name:水産基盤整備調査委託事業

    Awarding organization:水産庁

    鳥羽光晴, 宮﨑勝己他

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    Authorship:Coinvestigator(s) 

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  • アサリに寄生し漁業被害を与えるカイヤドリウミグモに関する基礎生物学的研究

    Grant number:20658045

    2008 - 2009

    System name:科学研究費助成事業 挑戦的萌芽研究

    Research category:挑戦的萌芽研究

    Awarding organization:日本学術振興会

    宮崎 勝己

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    Grant amount:\2500000 ( Direct Cost: \2500000 )

    アサリ等の二枚貝に内部寄生し、多大な漁業被害を与えているカイヤドリウミクモ(以下「ウミグモ」と略する)について、その防除や被害拡大防止を目指した、基礎的な生物学的知見の集積を目的に行った。
    1. 後胚発生
    寄生直後の初期幼生の発生様式を調べるため、孵化幼生を未感染のアサリ殻内へピペットを用いて強制的に注入する、感染試験を行った。感染個体からウミグモを定期的に採取し、得られた初期幼生の胴部・腹部及び付属肢の発達状態から、孵化幼生と前年度確認した最小個体の間に、少なくとも3つの発生段階を認めた。これにより、少なくとも11の後胚発生段階が確認出来た。
    2. 卵形成と産卵
    成体雌性生殖器官系の組織学的観察を行った。卵巣の歩脚内分枝は各歩脚の第6節に達し、第4節までに止まる他のウミグモよりも長い。更に他のウミグモでは見られない卵巣全域での卵形成の進行を確認した。これらの事は、この種の高い卵生産能を示している。卵巣中の卵細胞は、組織学的に「卵原細胞」「前卵黄形成期卵母細胞」「卵黄形成期卵母細胞」の3段階に分けられた。卵原細胞は卵巣全域に分布し、形成細胞巣を形作っていた。卵母細胞は卵巣外側の血体腔中に突出し、その位置で卵形成が進行していた。輸卵管を介した、卵巣腔と生殖孔の直接の連絡が確認出来た。これらの生殖器官系の構造と卵形成様式は、基本的に他のウミグモと同様である。飼育下で産卵した直後の卵巣中には、前述の3段階の卵細胞とも存在していた事から、複数回の産卵が可能であると結論された。
    3. 抗体作成
    迅速な対策と被害拡大防止につながると期待されるウミグモ孵化幼生の早期発見を目指し、抗ウミグモ抗体の作成を試みた。作成は(株)セシルリサーチ社に委託した。特異性が認められる抗血清の作成に成功したが、より特異性の高いモノクロナール抗体の作成は、十分な活性を示すハイブリドーマのラインが得られず、断念した。

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  • 日本産ウミグモ類のチェックリスト作成及び東アジア産ウミグモ類の生物地理に関する予備的研究

    2005.4 - 2006.3

    System name:学術研究助成

    Awarding organization:(財)藤原ナチュラルヒストリー振興財団

    宮﨑勝己

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  • On the evolution of mode of development in arthropods using pycnogonids and tardigrades

    Grant number:17570081

    2005 - 2007

    System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    MIYAZAKI Katsumi, SUZUKI Atsushi, IKUTA Kyosuke

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    Grant amount:\3750000 ( Direct Cost: \3600000 、 Indirect Cost:\150000 )

    Considering the phylogeny and evolution of arthropods and ecdysozoans, pycnogonids and tardigrades would be the "key-taxa". The present study aims to accumulate the basic information on their development to understand the evolution of its mode.
    We obtain the following results throughout the term of the grant.
    1. The ultrastructure of the oogonial cluster are described and the lack of nurse cells are confirmed for the first time in pycnogonids. They show that the oogenetic mode of pycnogonids is primitive among arthropods.
    2. The ultrastructural observation reveals that the mode of vitelline membrane formation in pycnogonids is very primitive among arthropods.
    3. The method for the serial resin sections for the yolk-rich pycnogonid eggs and embryos is established.
    4. The differences of internal structure between the two types of pycnogonid larvae, the protonymphon and the attaching larvae are described.
    5. The basic data of the embryo of a pycnogonid, Nymphonella tapetis such as the term of embryogenesis is obtained.
    6. The ultrastructural observation reveals the nature of the "nurse cells" of an ostracod, Cyprinotus uenoi.
    7. The culture system of a tardigrade, Milnesium tardigradum is established.
    8. The basic data of the embryo of Milnesium tardigradum such as the term of embryogenesis is obtained from the culture.
    9. The ultrastructure of the ovary and the mode of oogenesis in two species of tardigrades, Actinarctus doryphorus and Milnesium tardigradum belonging to the different classes are described.
    10. engrailed gene of Milnesium tardigradum is cloned and sequenced partly.
    11. The cultured specimens of Milnesium tardigradum are provided for a research on the molecular evolution of the Msx protein among Metazoa.

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  • 節足動物鋏角類循環器官系に関する比較形態学的研究

    2002.4 - 2004.3

    System name:研究助成

    Awarding organization:(公)成茂動物科学振興基金

    宮﨑勝己

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  • サンゴイソギンチャクに内部寄生するウミグモの生活史と後胚発生に関する研究

    2001.4 - 2002.3

    System name:個別研究助成

    Awarding organization:(財)水産無脊椎動物研究所

    宮﨑勝己

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  • Research on diversity and evolution of ovarian structure and oogenenc mode in arthropods

    Grant number:13640695

    2001 - 2003

    System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    MIYAZAKI Katsumi, ANDO Hitoshi, YAHATA Kensuke, TSUTSUMI Tadaaki, IKUTA Kyosuke

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    Grant amount:\3000000 ( Direct Cost: \3000000 )

    In order to understand the diversity of ovarian structure and oogenesis in arthropods, we made a comparative morphological observations especially on the groups where no reliable information on these characteristics was available.
    In chelicerates, we described the female reproductive system of a schizomid in detail for the first time (Miyazaki et al., '01), and the outline of life history of a pseudoscorpion based on the seasonal change of ovarian histology (Kato & Tsutsumi, in press).
    In pycnogonids, we ascertained the position of their gonads was unique among arthropods (Miyazaki & Pass, in press). The deviated position of genital pores of an austorodecid pycnogonid was corrected (Miyazaki, in press).
    In insects, the ultrastructure of egg membrane of a mantophasmid was examined for the first time, and some unique characteristics were described (Tsutsumi et al., in press). The oogenesis and postembryonic development of ovary were compared between, oviparous and ovoviviparous mayflies, and some differences probably reflected their distinct reproductive modes were found (Takahashi & Tsutsumi, 'O1, '02; Kageyama & Tsutsumi, in press).
    In myriapods, a comprehensive observation was made on diplopods, and the basic structures of ovary were inferred in their main groups.
    In crustaceans, the chelicerate-like ovaries in some groups of ostracods were shown to be evolved convergently. Also the ovarian structure in astacids, the postembryonic development of gonads in notostracans (Mitsumoto & Makioka, '02, '03), and the structure of gonads in an epicarid (Matsuzawa & Yahata, '03) were described.

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  • ツメナガウミグモ(節足動物門;ウミグモ網)の後胚発生に関する研究

    Grant number:08740667

    1996

    System name:科学研究費助成事業 奨励研究(A)

    Research category:奨励研究(A)

    Awarding organization:日本学術振興会

    宮崎 勝己

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    Grant amount:\1000000 ( Direct Cost: \1000000 )

    材料のツメナガウミグモは、全て伊豆・下田近海より春季にドレッジにより採集し、雄成体が抱える受精卵及び初期幼生を、過去の方法に従い20℃の恒温器内で、アルテミア幼生を餌として止水飼育し、得られた各後胚発生段階(9令まで)の幼生を組織切片及び走査型電子顕微鏡用試料とした。飼育中途で死亡する個体が、予想以上に多く出たため、8令以降の後期幼生は、数個体づつしか得られなかったため、冬季に追加採集を行い、補填した。
    このウミグモについては、成体における雌性生殖器官系の形態が、他のウミグモのそれと大きく異なっていることが、研究代表者により既に観察されていたが(宮崎,投稿準備中)、今回の研究によって、後期幼生の段階で、他のウミグモで普通にみられる形態を経ることがわかり、成体における特殊な形態が、二次的に変形したものであることが、明確に示された(宮崎,投稿準備中)。
    その他、ウミグモ類の系統進化的観点より特に注目していた、頭部付属肢と合体節の形成過程については、それぞれ3令から4令にかけて、外部形態、内部形態ともに劇的に変化することがわかったが、変化があまりにも急激なため、現時点ではその過程を追跡しきれておらず、現在、該当する時期の幼生の組織切片を順次作成して、解析を行っている。
    以上のように、研究期間の短さから、解析が不十分な点も残ったが、いくつかの新知見とともに、今後更に詳しい観察を行うための基礎的なデータを、得ることができた。

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Teaching Experience

  • 自然環境科学研究演習

    2023
    Institution name:新潟大学

  • 生物学実験

    2023
    Institution name:新潟大学

  • 理学基礎演習

    2022
    Institution name:新潟大学

  • 科学・技術と社会

    2021
    Institution name:新潟大学

  • 多様性生物学特論I

    2021
    Institution name:新潟大学

  • 理学スタディ・スキルズ

    2021
    Institution name:新潟大学

  • 課題研究C

    2021
    Institution name:新潟大学

  • 課題研究B

    2021
    Institution name:新潟大学

  • 課題研究(自然環境)B

    2020
    Institution name:新潟大学

  • 環境汚染論

    2020
    Institution name:新潟大学

  • 課題研究(自然環境)C

    2020
    -
    2021
    Institution name:新潟大学

  • 専門力アクティブ・ラーニング

    2020
    -
    2021
    Institution name:新潟大学

  • 課題研究

    2020
    -
    2021
    Institution name:新潟大学

  • 多様性生物学C

    2020
    -
    2021
    Institution name:新潟大学

  • 課題研究(自然環境)A

    2020
    -
    2021
    Institution name:新潟大学

  • 自然環境科学

    2020
    Institution name:新潟大学

  • 自然環境科学実験B2

    2019
    Institution name:新潟大学

  • 自然環境科学実験B1

    2018
    Institution name:新潟大学

  • 環境生物学野外実習B

    2018
    Institution name:新潟大学

  • 生物学基礎実習b

    2018
    Institution name:新潟大学

  • 自然環境科学総論

    2018
    Institution name:新潟大学

  • 多様性生物学B

    2018
    Institution name:新潟大学

  • 安全教育

    2018
    Institution name:新潟大学

  • 生物学基礎実習a

    2018
    Institution name:新潟大学

  • 環境生物学演習

    2017
    Institution name:新潟大学

  • 生物学基礎A

    2017
    Institution name:新潟大学

  • 適応生物学

    2017
    Institution name:新潟大学

  • 自然科学基礎実験

    2017
    Institution name:新潟大学

  • 課題研究B

    2017
    -
    2021
    Institution name:新潟大学

  • 課題研究C

    2017
    -
    2021
    Institution name:新潟大学

  • 課題研究A

    2017
    -
    2021
    Institution name:新潟大学

  • 生物学実験 I

    2017
    -
    2019
    Institution name:新潟大学

  • 自然環境科学実験B

    2017
    -
    2018
    Institution name:新潟大学

  • 自然科学実験法

    2017
    Institution name:新潟大学

  • 理学スタディ・スキルズ

    2017
    Institution name:新潟大学

  • 進化生物学特論II

    2016
    Institution name:新潟大学

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