2021/10/20 更新

写真a

ハナマタ シゲル
花俣 繁
HANAMATA Shigeru
所属
教育研究院 自然科学系 特任助教
農学部 特任助教
職名
特任助教
外部リンク

学位

  • 博士(理学) ( 2013年3月   東京理科大学 )

  • 修士(バイオサイエンス) ( 2009年3月   帝京科学大学 )

  • 学士(理学) ( 2007年3月   帝京科学大学 )

研究キーワード

  • 植物生理

  • 植物細胞分子生物

  • バイオイメージング

研究分野

  • ライフサイエンス / 植物分子、生理科学

経歴(researchmap)

  • 新潟大学   農学部   特任助教

    2018年4月 - 現在

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  • 東京理科大学 研究推進機構 総合研究院 イメージングフロンティアセンター   ポストドクトラル研究員

    2015年11月 - 2018年3月

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  • 東京理科大学 理工学部 応用生物科学科   ポストドクトラル研究員

    2015年4月 - 2015年10月

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  • 東京大学 大学院新領域創成科学研究科   特任研究員

    2014年4月 - 2015年3月

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  • 東京理科大学   理工学部 応用生物科学科   奨励研究員

    2013年4月 - 2014年3月

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経歴

  • 新潟大学   農学部   特任助教

    2018年4月 - 現在

所属学協会

  • 日本植物学会

    2017年9月 - 現在

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  • 日本バイオイメージング学会

    2009年4月 - 現在

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  • 日本植物生理学会

    2009年3月 - 現在

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  • 日本植物バイオテクノロジー学会

    2008年9月 - 現在

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論文

  • Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells. 査読 国際誌

    Shigeru Hanamata, Takamitsu Kurusu, Kazuyuki Kuchitsu

    International journal of molecular sciences   21 ( 23 )   2020年12月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Autophagy is ubiquitous in eukaryotic cells and plays an essential role in stress adaptation and development by recycling nutrients and maintaining cellular homeostasis. However, the dynamics and regulatory mechanisms of autophagosome formation during the cell cycle in plant cells remain poorly elucidated. We here analyzed the number of autophagosomes during cell cycle progression in synchronized tobacco BY-2 cells expressing YFP-NtATG8a as a marker for the autophagosomes. Autophagosomes were abundant in the G2 and G1 phases of interphase, though they were much less abundant in the M and S phases. Autophagosomes drastically decreased during the G2/M transition, and the CDK inhibitor roscovitine inhibited the G2/M transition and the decrease in autophagosomes. Autophagosomes were rapidly increased by a proteasome inhibitor, MG-132. MG-132-induced autophagosome formation was also markedly lower in the M phases than during interphase. These results indicate that the activity of autophagosome formation is differently regulated at each cell cycle stage, which is strongly suppressed during mitosis.

    DOI: 10.3390/ijms21239166

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  • Actin Filament Disruption Alters Phragmoplast Microtubule Dynamics during the Initial Phase of Plant Cytokinesis. 査読

    Keisho Maeda, Michiko Sasabe, Shigeru Hanamata, Yasunori Machida, Seiichiro Hasezawa, Takumi Higaki

    Plant & cell physiology   61 ( 3 )   445 - 456   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Plant growth and development relies on the accurate positioning of the cell plate between dividing cells during cytokinesis. The cell plate is synthetized by a specialized structure called the phragmoplast, which contains bipolar microtubules that polymerize to form a framework with the plus ends at or near the division site. This allows the transport of Golgi-derived vesicles toward the plus ends to form and expand the cell plate. Actin filaments play important roles in cell plate expansion and guidance in plant cytokinesis at the late phase, but whether they are involved at the early phase is unknown. To investigate this further, we disrupted the actin filaments in cell cycle-synchronized tobacco BY-2 cells with latrunculin B (LatB), an actin polymerization inhibitor. We observed the cells under a transmission electron microscope or a spinning-disk confocal laser scanning microscope. We found that disruption of actin filaments by LatB caused the membrane vesicles at the equatorial plane of the cell plate to be dispersed rather than form clusters as they did in the untreated cells. The midzone constriction of phragmoplast microtubules also was perturbed in LatB-treated cells. The live cell imaging and kymograph analysis showed that disruption of actin filaments also changed the accumulation timing of NACK1 kinesin, which plays a crucial role in cell plate expansion. This suggests that there are two functionally different types of microtubules in the phragmoplast. Together, our results show that actin filaments regulate phragmoplast microtubules at the initial phase of plant cytokinesis.

    DOI: 10.1093/pcp/pcaa003

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  • Impact of Autophagy on Gene Expression and Tapetal Programmed Cell Death During Pollen Development in Rice. 査読 国際誌

    Shigeru Hanamata, Jumpei Sawada, Seijiro Ono, Kazunori Ogawa, Togo Fukunaga, Ken-Ichi Nonomura, Seisuke Kimura, Takamitsu Kurusu, Kazuyuki Kuchitsu

    Frontiers in plant science   11   172 - 172   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Autophagy has recently been shown to be required for tapetal programmed cell death (PCD) and pollen maturation in rice. A transcriptional regulatory network is also known to play a key role in the progression of tapetal PCD. However, the relationship between the gene regulatory network and autophagy in rice anther development is mostly unknown. Here, we comprehensively analyzed the effect of autophagy disruption on gene expression profile during the tapetal PCD in rice anther development using high-throughput RNA sequencing. Expression of thousands of genes, including specific transcription factors and several proteases required for tapetal degradation, fluctuated synchronously at specific stages during tapetal PCD progression in the wild-type anthers, while this fluctuation showed significant delay in the autophagy-deficient mutant Osatg7-1. Moreover, gene ontology enrichment analysis in combination with self-organizing map clustering as well as pathway analysis revealed that the expression patterns of a variety of organelle-related genes as well as genes involved in carbohydrate/lipid metabolism were affected in the Osatg7-1 mutant during pollen maturation. These results suggest that autophagy is required for proper regulation of gene expression and quality control of organelles and timely progression of tapetal PCD during rice pollen development.

    DOI: 10.3389/fpls.2020.00172

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  • Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation. 査読

    Sera Y, Hanamata S, Sakamoto S, Ono S, Kaneko K, Mitsui Y, Koyano T, Fujita N, Sasou A, Masumura T, Saji H, Nonomura KI, Mitsuda N, Mitsui T, Kurusu T, Kuchitsu K

    Scientific reports   9 ( 1 )   18544   2019年12月

  • Monitoring autophagy in rice tapetal cells during pollen maturation. 査読

    Hanamata S, Sawada J, Toh B, Ono S, Ogawa K, Fukunaga T, Nonomura KI, Kurusu T, Kuchitsu K

    Plant biotechnology (Tokyo, Japan)   36 ( 2 )   99 - 105   2019年

  • イネの生殖過程におけるオートファジーの重要性 招待 査読

    来須孝光, 花俣繁, 瀬良ゆり, 朽津和幸

    日本植物学会「植物科学の最前線(BSJ-Review)」   9   29 - 35   2018年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.24480/bsj-review.9a5.00131

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  • Autophagy-mediated regulation of phytohormone metabolism during rice anther development 査読

    Takamitsu Kurusu, Tomoko Koyano, Nobutaka Kitahata, Mikiko Kojima, Shigeru Hanamata, Hitoshi Sakakibara, Kazuyuki Kuchitsu

    Plant Signaling and Behavior   12 ( 9 )   e1365211   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Taylor and Francis Inc.  

    Autophagy has recently been shown to be required for postmeiotic anther development including anther dehiscence, programmed cell death-mediated degradation of the tapetum and pollen maturation in rice. Several phytohormones are known to play essential roles during male reproductive development including pollen maturation. However, the relationship between phytohormone metabolism and autophagy in plant reproductive development is unknown. We here comprehensively analyzed the effect of autophagy disruption on phytohormone contents in rice anthers at the flowering stage, and found that endogenous levels of active-forms of gibberellins (GAs) and cytokinin, trans-zeatin, were significantly lower in the autophagy-defective mutant, Osatg7–1, than in the wild type. Treatment with GA4 partially recovered maturation of the mutant pollens, but did not recover the limited anther dehiscence as well as sterility phenotype. These results suggest that autophagy affects metabolism and endogenous levels of GAs and cytokinin in rice anthers. Reduction in bioactive GAs in the autophagy-deficient mutant may partially explain the defects in pollen maturation of the autophagy-deficient mutant, but tapetal autophagy also plays other specific roles in fertilization.

    DOI: 10.1080/15592324.2017.1365211

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  • 植物の感染防御応答の制御と細胞内の膜動態 招待 査読

    朽津和幸, 大滝幹, 羽山大介, 北畑信隆, 花俣繁, 来須孝光, 上田貴志

    バイオイメージング   25 ( 1 )   31 - 31   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • Quantitative live cell imaging of autophagic flux and roles of autophagy in reproductive development in plants. 査読

    Kurusu, T, Hanamata, S, Kuchitsu, K

    Bioimages   24 ( 1 )   1 - 11   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.11169/bioimages.24.1

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  • イネの生殖器官発達におけるオートファジーの新たな役割 査読

    来須 孝光, 花俣 繁, 小谷野 智子, 朽津 和幸

    バイオイメージング   24 ( 1 )   7 - 11   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • Autophagy Supports Biomass Production and Nitrogen Use Efficiency at the Vegetative Stage in Rice 査読

    Shinya Wada, Yasukazu Hayashida, Masanori Izumi, Takamitsu Kurusu, Shigeru Hanamata, Keiichi Kanno, Soichi Kojima, Tomoyuki Yamaya, Kazuyuki Kuchitsu, Amane Makino, Hiroyuki Ishida

    PLANT PHYSIOLOGY   168 ( 1 )   60 - U721   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Much of the nitrogen in leaves is distributed to chloroplasts, mainly in photosynthetic proteins. During leaf senescence, chloroplastic proteins, including Rubisco, are rapidly degraded, and the released nitrogen is remobilized and reused in newly developing tissues. Autophagy facilitates the degradation of intracellular components for nutrient recycling in all eukaryotes, and recent studies have revealed critical roles for autophagy in Rubisco degradation and nitrogen remobilization into seeds in Arabidopsis (Arabidopsis thaliana). Here, we examined the function of autophagy in vegetative growth and nitrogen usage in a cereal plant, rice (Oryza sativa). An autophagy-disrupted rice mutant, Osatg7-1, showed reduced biomass production and nitrogen use efficiency compared with the wild type. While Osatg7-1 showed early visible leaf senescence, the nitrogen concentration remained high in the senescent leaves. N-15 pulse chase analysis revealed suppression of nitrogen remobilization during leaf senescence in Osatg7-1. Accordingly, the reduction of nitrogen available for newly developing tissues in Osatg7-1 likely led its reduced leaf area and tillers. The limited leaf growth in Osatg7-1 decreased the photosynthetic capacity of the plant. Much of the nitrogen remaining in senescent leaves of Osatg7-1 was in soluble proteins, and the Rubisco concentration in senescing leaves of Osatg7-1 was about 2.5 times higher than in the wild type. Transmission electron micrographs showed a cytosolic fraction rich with organelles in senescent leaves of Osatg7-1. Our results suggest that autophagy contributes to efficient nitrogen remobilization at the whole-plant level by facilitating protein degradation for nitrogen recycling in senescent leaves.

    DOI: 10.1104/pp.15.00242

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  • Roles of autophagy in male reproductive development in plants 査読

    Shigeru Hanamata, Takamitsu Kurusu, Kazuyuki Kuchitsu

    FRONTIERS IN PLANT SCIENCE   5   457   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS RESEARCH FOUNDATION  

    Autophagy, a major catabolic pathway in eukaryotic cells, is essential in development, maintenance of cellular homeostasis, immunity and programmed cell death (PCD) in multicellular organisms. In plant cells, autophagy plays roles in recycling of proteins and metabolites including lipids, and is involved in many physiological processes such as abiotic and biotic stress responses. However, its roles during reproductive development had remained poorly understood. Quantitative live cell imaging techniques for the autophagic flux and genetic studies in several plant species have recently revealed significant roles of autophagy in developmental processes, regulation of PCD and lipid metabolism. We here review the novel roles of autophagic fluxes in plant cells, and discuss their possible significance in PCD and metabolic regulation, with particular focus on male reproductive development during the pollen maturation.

    DOI: 10.3389/fpls.2014.00457

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  • OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development 査読

    Takamitsu Kurusu, Tomoko Koyano, Shigeru Hanamata, Takahiko Kubo, Yuhei Noguchi, Chikako Yagi, Noriko Nagata, Takashi Yamamoto, Takayuki Ohnishi, Yozo Okazaki, Nobutaka Kitahata, Daichi Ando, Masaya Ishikawa, Shinya Wada, Akio Miyao, Hirohiko Hirochika, Hiroaki Shimada, Amane Makino, Kazuki Saito, Hiroyuki Ishida, Tetsu Kinoshita, Nori Kurata, Kazuyuki Kuchitsu

    AUTOPHAGY   10 ( 5 )   878 - 888   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS INC  

    In flowering plants, the tapetum, the innermost layer of the anther, provides both nutrient and lipid components to developing microspores, pollen grains, and the pollen coat. Though the programmed cell death of the tapetum is one of the most critical and sensitive steps for fertility and is affected by various environmental stresses, its regulatory mechanisms remain mostly unknown. Here we show that autophagy is required for the metabolic regulation and nutrient supply in anthers and that autophagic degradation within tapetum cells is essential for postmeiotic anther development in rice. Autophagosome-like structures and several vacuole-enclosed lipid bodies were observed in postmeiotic tapetum cells specifically at the uninucleate stage during pollen development, which were completely abolished in a retrotransposon-insertional OsATG7 (autophagy-related 7)-knockout mutant defective in autophagy, suggesting that autophagy is induced in tapetum cells. Surprisingly, the mutant showed complete sporophytic male sterility, failed to accumulate lipidic and starch components in pollen grains at the flowering stage, showed reduced pollen germination activity, and had limited anther dehiscence. Lipidomic analyses suggested impairment of editing of phosphatidylcholines and lipid desaturation in the mutant during pollen maturation. These results indicate a critical involvement of autophagy in a reproductive developmental process of rice, and shed light on the novel autophagy-mediated regulation of lipid metabolism in eukaryotic cells.

    DOI: 10.4161/auto.28279

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  • Regulation of vacuole-mediated programmed cell death during innate immunity and reproductive development in plants. In: Sexual Reproduction in Animals and Plants 招待 査読

    Koyano T, Kurusu T, Hanamata S, Kuchitsu K

    Springer   431 - 440   2014年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    DOI: 10.1007/978-4-431-54589-7_36

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  • 植物のオートファジー動態の簡便な定量解析 査読

    花俣 繁, 来須 孝光, 朽津 和幸

    バイオイメージング   23 ( 1 )   23 - 27   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • A low temperature-inducible protein AtSRC2 enhances the ROS-producing activity of NADPH oxidase AtRbohF. 査読

    Kawarazaki T, Kimura S, Iizuka A, Hanamata S, Nibori H, Michikawa M, Imai A, Abe M, Kaya H, Kuchitsu K

    Biochimica et biophysica acta   1833 ( 12 )   2775 - 2780   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbamcr.2013.06.024

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  • An S-Type Anion Channel SLAC1 Is Involved in Cryptogein-Induced Ion Fluxes and Modulates Hypersensitive Responses in Tobacco BY-2 Cells 査読

    Takamitsu Kurusu, Katsunori Saito, Sonoko Horikoshi, Shigeru Hanamata, Juntaro Negi, Chikako Yagi, Nobutaka Kitahata, Koh Iba, Kazuyuki Kuchitsu

    PLOS ONE   8 ( 8 )   e70623   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Pharmacological evidence suggests that anion channel-mediated plasma membrane anion effluxes are crucial in early defense signaling to induce immune responses and hypersensitive cell death in plants. However, their molecular bases and regulation remain largely unknown. We overexpressed Arabidopsis SLAC1, an S-type anion channel involved in stomatal closure, in cultured tobacco BY-2 cells and analyzed the effect on cryptogein-induced defense responses including fluxes of Cl- and other ions, production of reactive oxygen species (ROS), gene expression and hypersensitive responses. The SLAC1-GFP fusion protein was localized at the plasma membrane in BY-2 cells. Overexpression of SLAC1 enhanced cryptogein-induced Cl- efflux and extracellular alkalinization as well as rapid/transient and slow/prolonged phases of NADPH oxidase-mediated ROS production, which was suppressed by an anion channel inhibitor, DIDS. The overexpressor also showed enhanced sensitivity to cryptogein to induce downstream immune responses, including the induction of defense marker genes and the hypersensitive cell death. These results suggest that SLAC1 expressed in BY-2 cells mediates cryptogein-induced plasma membrane Cl- efflux to positively modulate the elicitor-triggered activation of other ion fluxes, ROS as well as a wide range of defense signaling pathways. These findings shed light on the possible involvement of the SLAC/SLAH family anion channels in cryptogein signaling to trigger the plasma membrane ion channel cascade in the plant defense signal transduction network.

    DOI: 10.1371/journal.pone.0070623

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  • タバコ培養細胞のオートファジー動態に関する研究:定量的解析法の開発と、細胞周期・感染シグナルの影響の解析 査読

    花俣 繁

    2013年3月

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    記述言語:日本語   掲載種別:学位論文(博士)  

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  • In vivo imaging and quantitative monitoring of autophagic flux in tobacco BY-2 cells 査読

    Shigeru Hanamata, Takamitsu Kurusu, Masaaki Okada, Akiko Suda, Koki Kawamura, Emi Tsukada, Kazuyuki Kuchitsu

    Plant Signaling and Behavior   8 ( 1 )   53 - 63   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Autophagy has been shown to play essential roles in the growth, development and survival of eukaryotic cells. However, simple methods for quantification and visualization of autophagic flux remain to be developed in living plant cells. Here, we analyzed the autophagic flux in transgenic tobacco BY-2 cell lines expressing fluorescence-tagged NtATG8a as a marker for autophagosome formation. Under sucrose-starved conditions, the number of punctate signals of YFP-NtATG8a increased, and the fluorescence intensity of the cytoplasm and nucleoplasm decreased. Conversely, these changes were not observed in BY-2 cells expressing a C-terminal glycine deletion mutant of the NtATG8a protein (NtATG8aΔG). To monitor the autophagic flux more easily, we generated a transgenic BY-2 cell line expressing NtATG8a fused to a pH-sensitive fluorescent tag, a tandem fusion of the acid-insensitive RFP and the acid-sensitive YFP. In sucrose-rich conditions, both fluorescent signals were detected in the cytoplasm and only weakly in the vacuole. In contrast, under sucrose-starved conditions, the fluorescence intensity of the cytoplasm decreased, and the RFP signal clearly increased in the vacuole, corresponding to the fusion of the autophagosome to the vacuole and translocation of ATG8 from the cytoplasm to the vacuole. Moreover, we introduce a novel simple easy way to monitor the autophagic flux non-invasively by only measuring the ratio of fluorescence of RFP and YFP in the cell suspension using a fluorescent image analyzer without microscopy. The present in vivo quantitative monitoring system for the autophagic flux offers a powerful tool for determining the physiological functions and molecular mechanisms of plant autophagy induced by environmental stimuli. © 2013 Landes Bioscience.

    DOI: 10.4161/psb.22510

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  • Involvement of the putative Ca²⁺-permeable mechanosensitive channels, NtMCA1 and NtMCA2, in Ca²⁺ uptake, Ca²⁺-dependent cell proliferation and mechanical stress-induced gene expression in tobacco (Nicotiana tabacum) BY-2 cells. 査読

    Kurusu T, Yamanaka T, Nakano M, Takiguchi A, Ogasawara Y, Hayashi T, Iida K, Hanamata S, Shinozaki K, Iida H, Kuchitsu K

    Journal of plant research   125 ( 4 )   555 - 568   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s10265-011-0462-6

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  • Regulation of Microbe-Associated Molecular Pattern-Induced Hypersensitive Cell Death, Phytoalexin Production, and Defense Gene Expression by Calcineurin B-Like Protein-Interacting Protein Kinases, OsCIPK14/15, in Rice Cultured Cells 査読

    Takamitsu Kurusu, Jumpei Hamada, Hiroshi Nokajima, Youichiro Kitagawa, Masahiro Kiyoduka, Akira Takahashi, Shigeru Hanamata, Ryoko Ohno, Teruyuki Hayashi, Kazunori Okada, Jinichiro Koga, Hirohiko Hirochika, Hisakazu Yamane, Kazuyuki Kuchitsu

    PLANT PHYSIOLOGY   153 ( 2 )   678 - 692   2010年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Although cytosolic free Ca2+ mobilization induced by microbe/pathogen-associated molecular patterns is postulated to play a pivotal role in innate immunity in plants, the molecular links between Ca2+ and downstream defense responses still remain largely unknown. Calcineurin B-like proteins (CBLs) act as Ca2+ sensors to activate specific protein kinases, CBL-interacting protein kinases (CIPKs). We here identified two CIPKs, OsCIPK14 and OsCIPK15, rapidly induced by microbe-associated molecular patterns, including chitooligosaccharides and xylanase (Trichoderma viride/ethylene-inducing xylanase [TvX/EIX]), in rice (Oryza sativa). Although they are located on different chromosomes, they have over 95% nucleotide sequence identity, including the surrounding genomic region, suggesting that they are duplicated genes. OsCIPK14/15 interacted with several OsCBLs through the FISL/NAF motif in yeast cells and showed the strongest interaction with OsCBL4. The recombinant OsCIPK14/15 proteins showed Mn2+-dependent protein kinase activity, which was enhanced both by deletion of their FISL/NAF motifs and by combination with OsCBL4. OsCIPK14/15-RNAi transgenic cell lines showed reduced sensitivity to TvX/EIX for the induction of a wide range of defense responses, including hypersensitive cell death, mitochondrial dysfunction, phytoalexin biosynthesis, and pathogenesis-related gene expression. On the other hand, TvX/EIX-induced cell death was enhanced in OsCIPK15-overexpressing lines. Our results suggest that OsCIPK14/15 play a crucial role in the microbe-associated molecular pattern-induced defense signaling pathway in rice cultured cells.

    DOI: 10.1104/pp.109.151852

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  • Roles of calcineurin B-like protein-interacting protein kinases in innate immunity in rice 査読

    Takamitsu Kurusu, Jumpei Hamada, Haruyasu Hamada, Shigeru Hanamata, Kazuyuki Kuchitsu

    Plant Signaling and Behavior   5 ( 8 )   1045 - 1047   2010年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Landes Bioscience  

    Cytosolic free Ca2+ mobilization induced by microbe/pathogen-associated molecular patterns (MAMPs/PAMPs) plays key roles in plant innate immunity. However, components involved in Ca2+ signaling pathways still remain to be identified and possible involvement of the CBL (calcineurin B-like proteins)-CIPK (CBL-interacting protein kinases) system in biotic defense signaling have yet to be clarified. Recently we identified two CIPKs, OsCIPK14 and OsCIPK15, which are rapidly induced by MAMPs, involved in various MAMPinduced immune responses including defense-related gene expression, phytoalexin biosynthesis and hypersensitive cell death. MAMP-induced production of reactive oxygen species as well as cell browning were also suppressed in OsCIPK14/15-RNAi transgenic cell lines. Possible molecular mechanisms and physiological functions of the CIPKs in plant innate immunity are discussed. © 2010 Landes Bioscience.

    DOI: 10.4161/psb.5.8.12407

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▶ 全件表示

書籍等出版物

  • Regulation of vacuole-mediated programmed cell death during innate immunity and reproductive development in plants.

    Koyano, T, Kurusu, T, Hanamata, S, Kuchitsu, K( 担当: 共著)

    Springer  2014年 

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    記述言語:英語 著書種別:学術書

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MISC

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受賞

  • 日本バイオイメージング学会ベストイメージ・オリンパス賞

    2016年9月   日本バイオイメージング学会  

    来須孝光, 瀬良ゆり, 陶 文紀, 澤田隼平, 花俣 繁, 坂本真吾, 小野聖二郎, 岡咲洋三, 徳永京也, 小嶋美紀子, 榊原均, 斉藤和季, 野々村賢一, 小関泰之, 光田展隆, 多田雄一, 朽津和幸

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    受賞区分:国内学会・会議・シンポジウム等の賞  受賞国:日本国

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  • 日本バイオイメージング学会ベストイメージ・カールツァイス賞

    2012年8月   日本バイオイメージング学会  

    小谷野智子, 来須孝光, 花俣繁, 八木智華子, 池田晃子, 野口祐平, 永田典子, 朽津和幸

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    受賞区分:国内学会・会議・シンポジウム等の賞  受賞国:日本国

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