2022/12/10 更新

写真a

ミヤザワ ハルナ
宮沢 春菜
MIYAZAWA Haruna
所属
医歯学総合病院 臨床研究推進センター 特任助教
職名
特任助教
外部リンク

学位

  • 博士(歯学) ( 2013年3月   新潟大学 )

研究分野

  • 情報通信 / 生命、健康、医療情報学

  • ライフサイエンス / 保存治療系歯学  / 歯周病学

経歴(researchmap)

  • 新潟大学   医歯学総合病院 臨床研究推進センター企業治験部門   (部門長)特任助教

    2022年7月 - 現在

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  • 新潟大学   医歯学総合病院 臨床研究推進センター実施管理部門   (部門長)特任助教

    2021年4月 - 2022年6月

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  • 国立研究開発法人日本医療研究開発機構   医療機器・ヘルスケア事業部 医療機器研究開発課   主幹

    2020年4月 - 2021年3月

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  • 国立研究開発法人日本医療研究開発機構   戦略推進部 再生医療研究課   主幹

    2019年4月 - 2020年3月

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  • 新潟大学   医歯学総合病院 臨床研究推進センター 開発戦略部門   特任助教

    2018年11月 - 2019年3月

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  • 新潟大学医歯学総合病院   歯周病科   医員

    2014年12月 - 2018年10月

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  • 新潟大学医歯学総合研究科   歯周診断・再建学分野   研究員

    2014年4月 - 2014年11月

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経歴

  • 新潟大学   医歯学総合病院 臨床研究推進センター   特任助教

    2018年12月 - 現在

学歴

  • 新潟大学   医歯学総合研究科   歯周診断・再建学分野

    2010年4月 - 2014年3月

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  • 新潟大学   歯学部   歯学科

    2003年4月 - 2009年3月

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委員歴

  • 日本医療情報学会 栄養・運動・口腔保健・休養の自己管理のための保健医療情報研究会   幹事  

    2021年4月 - 2024年3月   

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  • 経済産業省事業 保健医療福祉リアルワールドデータ活用 促進のための国際標準化   「ヘルス&ケアデータプロセスモデル」国際規格開発委員会 委員  

    2021年 - 現在   

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取得資格

  • 歯科医師

 

論文

  • Effect of Locally Delivered Minocycline on the Profile of Subgingival Bacterial Genera in Patients with Periodontitis: A Prospective Pilot Study. 査読 国際誌

    Toshiya Morozumi, Yohei Nakayama, Satoshi Shirakawa, Kentaro Imamura, Kaname Nohno, Takatoshi Nagano, Haruna Miyazawa, Takahiro Hokari, Ryo Takuma, Shuntaro Sugihara, Kazuhiro Gomi, Atsushi Saito, Yorimasa Ogata, Motohiro Komaki

    Biomolecules   12 ( 5 )   2022年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This prospective pilot study aimed to evaluate the effect of minocycline-HCl ointment (MO), locally delivered as an adjunct to scaling and root planing (SRP), on subgingival microflora. A total of 59 periodontitis patients received SRP as an initial periodontal therapy. In the selected periodontal pockets with probing depths (PD) of 6-9 mm, the sites that exhibited a positive reaction following a bacterial test using an immunochromatographic device were subsequently treated with MO (SRP + MO group, n = 25). No additional treatment was performed at sites showing a negative reaction (SRP group, n = 34). In addition to subgingival plaque sampling, measurement of clinical parameters including PD, clinical attachment level (CAL), bleeding on probing (BOP), plaque index and gingival index (GI) were performed at baseline and 4 weeks after the initial periodontal therapy. The subgingival microflora were assessed by terminal restriction fragment-length polymorphism analysis. Relative to baseline values, the mean scores for PD-, CAL-, BOP-, and GI-sampled sites were significantly decreased post treatment in both groups (p < 0.01). The intra-comparisons showed a significant decrease in the counts of the genera Eubacterium, Parvimonas, Filifactor, Veillonella, Fusobacterium, Porphyromonas, Prevotella, and unknown species in the SRP + MO group (p < 0.05). Inter-comparisons indicated a significant decrease in the genera Veillonella in the SRP + MO group (p = 0.01). Combination therapy of SRP and local MO induced a change in the subgingival microbial community: particularly, the number of Veillonella spp. was markedly reduced.

    DOI: 10.3390/biom12050719

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  • The relationship between dental metal allergy, periodontitis, and palmoplantar pustulosis: An observational study. 査読

    Yurina Takaoka, Yosuke Akiba, Masako Nagasawa, Akiko Ito, Yukiko Masui, Nami Akiba, Kaori Eguchi, Haruna Miyazawa, Koichi Tabeta, Katsumi Uoshima

    Journal of prosthodontic research   66 ( 3 )   438 - 444   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: This study aimed to investigate the relationship between dental metal allergy, periodontitis, and palmoplantar pustulosis among patients from a dental metal allergy clinic over a period of 8 years. METHODS: This study included 436 patients who visited our dental metal allergy clinic between April 1, 2009 and March 31, 2016. Diagnoses of skin diseases, periodontal records, dental metal series patch test results, and electron probe microanalysis (EPMA) data were obtained from medical records. Relative risk (RR) values were estimated from these data. RESULTS: Of the 359 patients who underwent the patch test, 241 showed a positive reaction. Of the 187 patients who underwent EPMA, 113 had allergenic metals in their dental prostheses. These patients were suspected to have a dental metal allergy. Furthermore, 150 of the 436 patients were diagnosed with palmoplantar pustulosis (PPP). The RR of metal allergy between patients with PPP and healthy subjects was 3.88. The RR of periodontal disease between patients with PPP and PPP-negative patients in the national average was 2.54. CONCLUSIONS: In this study, both dental metal allergy and periodontitis showed a high RR for PPP.

    DOI: 10.2186/jpr.JPR_D_20_00307

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  • Impact of Local Drug Delivery of Minocycline on the Subgingival Microbiota during Supportive Periodontal Therapy: A Randomized Controlled Pilot Study. 国際誌

    Haruna Miyazawa, Takako Nakajima, Makoto Horimizu, Kazuhiro Okuda, Noriko Sugita, Kyoko Yamazaki, Lu Li, Yoshiko Hayashi-Okada, Takuya Arita, Misa Nishimoto, Mieko Nishida, Robert J Genco, Kazuhisa Yamazaki

    Dentistry journal   8 ( 4 )   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The aim of this study was to examine the effect of adjunct local minocycline administration on the microbiological parameters of subgingival plaque samples in the residual periodontal pockets. Ten chronic periodontitis patients under a supportive periodontal therapy regimen were recruited. After subgingival debridement, either 2% minocycline gel, Periocline™, (Test Group) or a placebo (Control Group) was administered to the selected sites once a week for three weeks. Subgingival plaque was collected at baseline, and at four weeks and eight weeks. The microbiological composition was analyzed by 16S ribosomal RNA sequencing. In the Test Group, α-diversity (evenness) decreased compared to the baseline (p = 0.005) and was lower compared to the control group at four weeks (p = 0.003). The microbial community composition between the two groups was significantly different at four weeks (p = 0.029). These changes were attributable to a decrease in the bacteria associated with periodontitis and an increase in the bacteria associated with periodontal health. Additionally, the improvement in bleeding on probing continued at eight weeks; however, there were little microbial effects of 2% minocycline gel observed at eight weeks. The control group demonstrated no change throughout the eight-week experimental period. Thus, local minocycline administration can change the subgingival microbial community of residual periodontal pockets.

    DOI: 10.3390/dj8040123

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  • Corrigendum to "Indirect regulation of PCSK9 gene in inflammatory response by <i>Porphyromonas gingivalis</i> infection" [Heliyon 5 (1) (January 2019) e01111]. 査読

    Yokoji-Takeuchi M, Tabeta K, Takahashi N, Arimatsu K, Miyazawa H, Matsuda-Matsukawa Y, Sato K, Yamada M, Yamazaki K

    Heliyon   5 ( 2 )   e01210   2019年2月

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  • Indirect regulation of PCSK9 gene in inflammatory response by <i>Porphyromonas gingivalis</i> infection. 査読 国際誌

    Yokoji-Takeuchi M, Tabeta K, Takahashi N, Arimatsu K, Miyazawa H, Matsuda-Matsukawa Y, Sato K, Yamada M, Yamazaki K

    Heliyon   5 ( 1 )   e01111   2019年1月

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  • <i>β</i> <sub>2</sub>-Microglobulin and Neutrophil Gelatinase-Associated Lipocalin, Potential Novel Urine Biomarkers in Periodontitis: A Cross-Sectional Study in Japanese. 査読 国際誌

    Nakajima M, Hosojima M, Tabeta K, Miyauchi S, Yamada-Hara M, Takahashi N, Miyazawa H, Matsuda-Matsukawa Y, Sato K, Sugita N, Komatsu Y, Ishikawa T, Akiishi K, Yamazaki K, Kato K, Saito A, Yoshie H

    International journal of dentistry   2019   1394678 - 1394678   2019年

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    記述言語:英語  

    DOI: 10.1155/2019/1394678

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  • Increased serum PCSK9, a potential biomarker to screen for periodontitis, and decreased total bilirubin associated with probing depth in a Japanese community survey 査読

    K. Tabeta, M. Hosojima, M. Nakajima, S. Miyauchi, H. Miyazawa, N. Takahashi, Y. Matsuda, N. Sugita, Y. Komatsu, K. Sato, T. Ishikawa, K. Akiishi, K. Yamazaki, K. Kato, A. Saito, H. Yoshie

    Journal of Periodontal Research   53 ( 3 )   446 - 456   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Munksgaard  

    Background and Objectives: Previous reports suggest that several serum biomarkers play roles in the pathogenesis, inflammatory response, and oxidative stress in periodontitis caused by bacterial infections, linking chronic periodontitis to atherosclerotic vascular disease (ASVD). The aim of this preliminary study was to investigate, in a Japanese cross-sectional community survey, potential serum biomarkers of periodontitis that are associated with ASVD and chronic periodontitis. Material and Methods: The study cohort included a total of 108 male subjects who underwent annual health examinations. Serum biomarkers (high-sensitivity C-reactive protein [hs-CRP], proprotein convertase subtilisin/kexin type 9 [PCSK9], interleukin-6, tumor necrosis factor-α, soluble CD14, myeloperoxidase, matrix metalloproteinase-3, adiponectin, total bilirubin [TBIL], and serum lipids) were analyzed to determine their association (if any) with periodontal parameters. Aortic stiffness was evaluated using the brachial-ankle aortic pulse wave velocity (PWV) index and the cardio-ankle vascular index (CAVI). Results: The concentrations of PCSK9 and hs-CRP were increased (P =.001 and.042, respectively), and the concentration of TBIL was decreased (P =.046), in subjects with periodontal disease (determined as a probing depth of ≥4 mm in at least one site) compared with periodontally healthy subjects. The ratio of low-density lipoprotein cholesterol (LDL-C) to high-density lipoprotein cholesterol and the concentrations of triglycerides, remnant-like particles-cholesterol, and oxidized LDL were elevated in subjects with periodontal disease compared with periodontally healthy subjects (P =.038,.007,.002, and.049, respectively). Multivariate regression analyses indicated that the number of sites with a pocket depth of ≥4 mm was associated with the concentration of PCSK9 and inversely associated with the concentration of TBIL independently (standardized β =.243, P =.040
    standardized β = −.443, P =.0002
    respectively). Analysis of receiver operating characteristic curves of PCSK9 indicated moderate accuracy for predicting the presence of disease sites (probing depth ≥ 4 mm) (area under the curve = 0.740). No significance in the values of PWV and CAVI was observed between subjects with periodontal disease and periodontally healthy subjects. Conclusion: In Japanese male subjects, the concentrations of serum PCSK9 and TBIL were correlated with periodontal parameters. Moreover, PCSK9 could be a candidate biomarker for diagnosing chronic periodontitis, and may also have potential to evaluate the risk for periodontitis to cause ASVD. Longitudinal studies of larger populations are necessary to confirm the exact association of periodontitis with increased serum PCSK9 and decreased TBIL.

    DOI: 10.1111/jre.12533

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  • Oral pathobiont induces systemic inflammation and metabolic changes associated with alteration of gut microbiota 査読

    Kei Arimatsu, Hitomi Yamada, Haruna Miyazawa, Takayoshi Minagawa, Mayuka Nakajima, Mark I. Ryder, Kazuyoshi Gotoh, Daisuke Motooka, Shota Nakamura, Tetsuya Iida, Kazuhisa Yamazaki

    SCIENTIFIC REPORTS   4   4828   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Periodontitis has been implicated as a risk factor for metabolic disorders such as type 2 diabetes, atherosclerotic vascular diseases, and non-alcoholic fatty liver disease. Although bacteremias from dental plaque and/or elevated circulating inflammatory cytokines emanating from the inflamed gingiva are suspected mechanisms linking periodontitis and these diseases, direct evidence is lacking. We hypothesize that disturbances of the gut microbiota by swallowed bacteria induce a metabolic endotoxemia leading metabolic disorders. To investigate this hypothesis, changes in the gut microbiota, insulin and glucose intolerance, and levels of tissue inflammation were analysed in mice after oral administration of Porphyromonas gingivalis, a representative periodontopathogens. Pyrosequencing revealed that the population belonging to Bacteroidales was significantly elevated in P. gingivalis-administered mice which coincided with increases in insulin resistance and systemic inflammation. In P. gingivalis-administered mice blood endotoxin levels tended to be higher, whereas gene expression of tight junction proteins in the ileum was significantly decreased. These results provide a new paradigm for the interrelationship between periodontitis and systemic diseases.

    DOI: 10.1038/srep04828

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  • Natural killer T cells mediate alveolar bone resorption and a systemic inflammatory response in response to oral infection of mice with Porphyromonas gingivalis 査読

    Y. Aoki-Nonaka, T. Nakajima, S. Miyauchi, H. Miyazawa, H. Yamada, H. Domon, K. Tabeta, K. Yamazaki

    JOURNAL OF PERIODONTAL RESEARCH   49 ( 1 )   69 - 76   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Background and Objective: T and B cells are known to be involved in the disease process of periodontitis. However, the role of natural killer T cells in the pathogenesis of periodontitis has not been clarified.
    Materials and Methods: To examine the role of these cells, C57BL/6J (wild-type), CD1d(-/-) and alpha-galactosylceramide (alpha GC)-stimulated wild-type mice were orally infected with Porphyromonas gingivalis strain W83.
    Results: Apart from CD1d(-/-) mice, the level of alveolar bone resorption was elevated by the infection and was further accelerated in alpha GC-stimulated mice. The infection induced elevated levels of serum amyloid A and P. gingivalis-specific IgG in the sera, although the degree of elevation was much smaller in the CD1d(-/-) mice. Infection-induced RANKL elevation was only observed in aGC-stimulated mice. Although the cytokines produced by splenocytes were mainly T-helper 1 type in wild-type mice, those in alpha GC-stimulated mice were predominantly T-helper 2 type. In the liver, the infection demonstrated no effect on the gene expression for interferon-gamma, interleukin-4 and RANKL except alpha GC-stimulated mice in which the infection upregulated the gene expressions.
    Conclusion: This study is the first to show that natural killer T cells upregulated systemic and local inflammatory responses induced by oral infection with P. gingivalis, thereby contributing to the progression of alveolar bone resorption.

    DOI: 10.1111/jre.12080

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  • Effect of Porphyromonas gingivalis infection on post-transcriptional regulation of the low-density lipoprotein receptor in mice 査読

    Haruna Miyazawa, Koichi Tabeta, Sayuri Miyauchi, Yukari Aoki-Nonaka, Hisanori Domon, Tomoyuki Honda, Takako Nakajima, Kazuhisa Yamazaki

    LIPIDS IN HEALTH AND DISEASE   11   121   2012年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Periodontal disease is suggested to increase the risk of atherothrombotic disease by inducing dyslipidemia. Recently, we demonstrated that proprotein convertase subtilisin/kexin type 9 (PCSK9), which is known to play a critical role in the regulation of circulating low-density lipoprotein (LDL) cholesterol levels, is elevated in periodontitis patients. However, the underlying mechanisms of elevation of PCSK9 in periodontitis patients are largely unknown. Here, we explored whether Porphyromonas gingivalis, a representative periodontopathic bacterium, -induced inflammatory response regulates serum PCSK9 and cholesterol levels using animal models.
    Methods: We infected C57BL/6 mice intraperitoneally with Porphyromonas gingivalis, a representative strain of periodontopathic bacteria, and evaluated serum PCSK9 levels and the serum lipid profile. PCSK9 and LDL receptor (LDLR) gene and protein expression, as well as liver X receptors (Lxrs), inducible degrader of the LDLR (Idol), and sterol regulatory element binding transcription factor (Srebf)2 gene expression, were examined in the liver.
    Results: P. gingivalis infection induced a significant elevation of serum PCSK9 levels and a concomitant elevation of total and LDL cholesterol compared with sham-infected mice. The LDL cholesterol levels were significantly correlated with PCSK9 levels. Expression of the Pcsk9, Ldlr, and Srebf2 genes was upregulated in the livers of the P. gingivalis-infected mice compared with the sham-infected mice. Although Pcsk9 gene expression is known to be positively regulated by sterol regulatory element binding protein (SREBP)2 (human homologue of Srebf2), whereas Srebf2 is negatively regulated by cholesterol, the elevated expression of Srebf2 found in the infected mice is thought to be mediated by P. gingivalis infection.
    Conclusions: P. gingivalis infection upregulates PCSK9 production via upregulation of Srebf2, independent of cholesterol levels. Further studies are required to elucidate how infection regulates Srebf2 expression and subsequently influences lipid metabolism.

    DOI: 10.1186/1476-511X-11-121

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  • Oral infection with Porphyromonas gingivalis and systemic cytokine profile in C57BL/6.KOR-ApoEshl mice 査読

    S. Miyauchi, T. Maekawa, Y. Aoki, H. Miyazawa, K. Tabeta, T. Nakajima, K. Yamazaki

    JOURNAL OF PERIODONTAL RESEARCH   47 ( 3 )   402 - 408   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Background and Objective: Periodontal infection affects atherosclerotic diseases, such as coronary heart diseases. Mouse models have revealed that oral infection with Porphyromonas gingivalis induces changes in inflammatory-and lipid metabolism-related gene expression, regardless of the development of atherosclerotic lesions. However, the serum protein expression profile in the oral infection model has not been investigated. The present study aimed to analyse the effect of oral infection with P. gingivalis on the expression levels of multiple cytokines in the serum in apolipoprotein E-deficient mice by using a cytokine antibody array. Material and Methods: C57BL/ 6. KOR-Apoe shl mice were orally infected with P. gingivalis five times at 3 day intervals and were then killed. Splenocytes were isolated and analysed for proliferative activity and immunoglobulin G (IgG) production in response to in vitro restimulation with P. gingivalis. The expression levels of various cytokines in the sera were analysed using a mouse antibody array glass chip. Results: Splenocytes from P. gingivalis-infected mice demonstrated significantly greater proliferation and IgG production in response to P. gingivalis compared with those from sham-infected mice. Antibody array analysis revealed the selective upregulation of matrix metalloproteinase 3, intercellular adhesion molecule 1, insulin-like growth factor binding protein 2 and chemokine (C-X-C motif) ligand 7 and the downregulation of interleukin-17, tumor necrosis factor-a and L-selectin. Conclusion: These data demonstrate that oral infection with P. gingivalis induces alterations in systemic cytokine production. These cytokines could play roles in the development not only of periodontitis but also of atherosclerosis.

    DOI: 10.1111/j.1600-0765.2011.01441.x

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  • Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration 査読

    Haruna Miyazawa, Tomoyuki Honda, Sayuri Miyauchi, Hisanori Domon, Takafumi Okui, Takako Nakajima, Koichi Tabeta, Kazuhisa Yamazaki

    CLINICA CHIMICA ACTA   413 ( 1-2 )   154 - 159   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Background: Periodontal disease increases the risk of atherothrombotic disease, and high concentrations of low density lipoprotein (LDL) cholesterol are considered to be involved: however, the underlying mechanisms are largely unknown. Recent studies demonstrated that proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a critical role in circulating LDL cholesterol concentrations. The aim of the present study is to analyze serum PCSK9 concentrations and their relation to lipoprotein concentrations in periodontitis patients.
    Methods: Sera were obtained from 40 periodontitis patients and 30 control subjects. PCSK9 concentrations, high-sensitivity C-reactive protein (hs-CRP), IL-6, TNF-alpha and Porphyromonas gingivalis antibodies were measured by ELISA, and lipid profiles were determined by a commercial laboratory.
    Results: Periodontitis patients demonstrated significantly higher serum antibody titer to P. gingivalis and hs-CRP concentrations than control subjects, suggesting infection with P. gingivalis and a systemic inflammatory response. PCSK9 concentrations in periodontitis patients were significantly higher than those in control subjects. However, the concentrations of total and LDL cholesterols were not significantly different between periodontitis patients and control subjects. Moreover, no correlations were observed between PCSK9 concentrations and lipid profiles.
    Conclusion: Periodontal infection upregulates PCSK9 production. However, further studies are required to elucidate how periodontal infection affects PCSK9 concentrations and subsequent lipid metabolism. (C) 2011 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.cca.2011.09.023

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  • Chronic Oral Infection with Porphyromonas gingivalis Accelerates Atheroma Formation by Shifting the Lipid Profile 査読

    Tomoki Maekawa, Naoki Takahashi, Koichi Tabeta, Yukari Aoki, Hirotaka Miyashita, Sayuri Miyauchi, Haruna Miyazawa, Takako Nakajima, Kazuhisa Yamazaki

    PLOS ONE   6 ( 5 )   e20240   2011年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Background: Recent studies have suggested that periodontal disease increases the risk of atherothrombotic disease. Atherosclerosis has been characterized as a chronic inflammatory response to cholesterol deposition in the arteries. Although several studies have suggested that certain periodontopathic bacteria accelerate atherogenesis in apolipoprotein E-deficient mice, the mechanistic link between cholesterol accumulation and periodontal infection-induced inflammation is largely unknown.
    Methodology/Principal Findings: We orally infected C57BL/6 and C57BL/6. KOR-Apoe(shl) (B6.Apoeshl) mice with Porphyromonas gingivalis, which is a representative periodontopathic bacterium, and evaluated atherogenesis, gene expression in the aorta and liver and systemic inflammatory and lipid profiles in the blood. Furthermore, the effect of lipopolysaccharide (LPS) from P. gingivalis on cholesterol transport and the related gene expression was examined in peritoneal macrophages. Alveolar bone resorption and elevation of systemic inflammatory responses were induced in both strains. Despite early changes in the expression of key genes involved in cholesterol turnover, such as liver X receptor and ATP-binding cassette A1, serum lipid profiles did not change with short-term infection. Long-term infection was associated with a reduction in serum high-density lipoprotein (HDL) cholesterol but not with the development of atherosclerotic lesions in wild-type mice. In B6.Apoeshl mice, long-term infection resulted in the elevation of very low-density lipoprotein (VLDL), LDL and total cholesterols in addition to the reduction of HDL cholesterol. This shift in the lipid profile was concomitant with a significant increase in atherosclerotic lesions. Stimulation with P. gingivalis LPS induced the change of cholesterol transport via targeting the expression of LDL receptor-related genes and resulted in the disturbance of regulatory mechanisms of the cholesterol level in macrophages.
    Conclusions/Significance: Periodontal infection itself does not cause atherosclerosis, but it accelerates it by inducing systemic inflammation and deteriorating lipid metabolism, particularly when underlying hyperlidemia or susceptibility to hyperlipidemia exists, and it may contribute to the development of coronary heart disease.

    DOI: 10.1371/journal.pone.0020240

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MISC

  • 運動・口腔保健・栄養・休養の自己管理のための保健医療情報 健康増進に寄与する口腔保健データ管理と活用の可能性

    宮沢 春菜

    医療情報学連合大会論文集   41回   456 - 456   2021年11月

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    記述言語:日本語   出版者・発行元:(一社)日本医療情報学会  

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  • 広汎型重度慢性歯周炎患者に歯周矯正を応用した一症例

    多部田康一, 宮澤春菜, 竹内麻衣, 松岸葵, 都野隆博

    日本歯周病学会会誌(Web)   62   2020年

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  • 歯周炎患者唾液細菌叢が腸内細菌叢に与える影響の解析

    山崎 恭子, 中島 貴子, 宮澤 春菜, 佐藤 圭祐, 高橋 直紀, 原 実生, 竹内 麻衣, 山崎 和久

    日本歯周病学会会誌   61 ( 秋季特別 )   141 - 141   2019年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周炎患者における腸内細菌叢の解析

    山崎 恭子, 中島 貴子, 宮沢 春菜, 伊藤 晴江, 佐藤 圭祐, 原 実生, 竹内 麻衣, 高橋 直紀, 森田 英利, 須田 亙, 服部 正平, 山崎 和久

    腸内細菌学雑誌   33 ( 2 )   116 - 116   2019年4月

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    記述言語:日本語   出版者・発行元:(公財)腸内細菌学会  

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  • P.gingivalis感染におけるPCSK9産生の誘導機構

    横地麻衣, 横地麻衣, 多部田康一, 高橋直紀, 高橋直紀, 宮澤春菜, 松田由実, 佐藤圭祐, 山田実生, 山田実生, SULIJAYA Benso, SULIJAYA Benso, 山崎和久

    新潟歯学会雑誌   48 ( 2 )   110 - 110   2018年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

    J-GLOBAL

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  • 歯周病患者における機能指標としての咀嚼機能検査の有用性について

    宮沢 春菜, 中島 貴子, 松川 由実, 清水 伸太郎, 古市 保志, 根本 英二, 高井 英樹, 中山 洋平, 小方 頼昌, 岩崎 拓也, 石原 裕一, 大井 麻子, 齋藤 淳, 藤原 千春, 村上 伸也, 畑中 加珠, 高柴 正悟, 武田 克浩, 藤田 剛, 栗原 英見, 山崎 和久

    日本歯周病学会会誌   60 ( 秋季特別 )   136 - 136   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 塩酸ミノサイクリン局所投与がサポーティブペリオドンタルセラピー(SPT)期歯周炎患者の歯肉縁下細菌叢に及ぼす影響(第II報)

    宮沢 春菜, 中島 貴子, 堀水 慎, 杉田 典子, 奥田 一博, 山崎 和久, Li Lu, Genco Robert

    日本歯周病学会会誌   59 ( 秋季特別 )   190 - 190   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周炎患者腸内細菌叢における口腔内由来細菌の比率

    山崎恭子, 中島貴子, 高橋直紀, 宮澤春菜, 皆川高嘉, 佐藤圭祐, 伊藤晴江, 山崎和久

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)   147th   88 (WEB ONLY) - 88   2017年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    J-GLOBAL

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  • 塩酸ミノサイクリン局所投与がサポーティブペリオドンタルセラピー(SPT)期歯周炎患者の歯肉縁下細菌叢に及ぼす影響(第I報)

    中島 貴子, 宮沢 春菜, 堀水 慎, 杉田 典子, 奥田 一博, 山崎 和久, Li Lu, Genco Robert

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   147回   195 - 195   2017年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 歯周炎患者における腸内細菌叢の解析

    中島貴子, 中島貴子, 高橋直紀, 高橋直紀, 高橋直紀, 皆川高嘉, 皆川高嘉, 宮沢春菜, 宮沢春菜, 伊藤晴江, 伊藤晴江, 佐藤圭祐, 佐藤圭祐, 山崎和久

    日本歯周病学会会誌(Web)   59 ( 春季特別 )   129 - 129   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    J-GLOBAL

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  • 細菌抗原によるPCSK9産生の誘導機構

    横地麻衣, 横地麻衣, 多部田康一, 宮澤春菜, 野中由香莉, 高橋直紀, 松田由実, 松田由実, 佐藤圭祐, 佐藤圭祐, 山田実生, 山田実生, 伊藤晴江, 中島貴子, 山崎和久

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)   145th   ROMBUNNO.P94 (WEB ONLY) - 145   2016年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    J-GLOBAL

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  • 歯周炎と血液・尿中バイオロジカルマーカーとの関連解析

    中島麻由佳, 中島麻由佳, 多部田康一, 宮内小百合, 杉田典子, 小松康高, 本田朋之, 高橋直紀, 宮澤春菜, 有松圭, 皆川高嘉, 松田由実, 松田由実, 佐藤圭祐, 佐藤圭祐, 山崎和久, 吉江弘正

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)   143rd   P126 (WEB ONLY) - 197   2015年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    J-GLOBAL

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  • 歯周炎患者におけるPISAと血中マーカーとの関連性

    本田 朋之, 宮沢 春菜, 野中 由香莉, 高橋 直紀, 多部田 康一, 中島 貴子, 山崎 和久

    日本歯周病学会会誌   57 ( 秋季特別 )   127 - 127   2015年8月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • フレアアウトを伴う広汎型重度慢性歯周炎患者に歯周矯正治療を行った一症例

    多部田 康一, 宮沢 春菜, 山崎 和久, 吉江 弘正

    日本歯周病学会会誌   57 ( 秋季特別 )   149 - 149   2015年8月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周炎患者におけるPISAと血中マーカーとの関連性

    本田朋之, 本田朋之, 宮沢春菜, 宮沢春菜, 野中由香莉, 野中由香莉, 高橋直紀, 高橋直紀, 多部田康一, 中島貴子, 山崎和久

    日本歯周病学会会誌(Web)   57   2015年

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  • Porphyromonas gingivalis経口投与によるマウス腸内細菌叢の変動と内毒素血症の関連

    有松圭, 有松圭, 山田ひとみ, 山田ひとみ, 宮内小百合, 宮澤春菜, 宮澤春菜, 中島麻由佳, 中島麻由佳, 多部田康一, 中島貴子, 山崎和久

    日本歯周病学会学術大会プログラムおよび講演抄録集   57th   2014年

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  • Porphyromonas gingivalis経口投与はマウス腸内細菌叢を変動させインスリン抵抗性を誘導する

    有松圭, 有松圭, 山田ひとみ, 山田ひとみ, 宮内小百合, 宮沢春菜, 中島麻由佳, 中島麻由佳, 多部田康一, 中島貴子, 山崎和久

    新潟歯学会雑誌   44 ( 2 )   2014年

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  • マウスPorphyromonas gingivalis口腔感染で誘導される小胞体ストレス応答と歯槽骨吸収の関連

    山田ひとみ, 山田ひとみ, 土門久哲, 宮内小百合, 宮澤春菜, 宮澤春菜, 多部田康一, 中島貴子, 山崎和久

    日本歯周病学会学術大会プログラムおよび講演抄録集   57th   2014年

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  • Porphyromonas gingivalis口腔感染マウスモデルにおいて小胞体ストレスは破骨細胞形成に関与し歯槽骨吸収を誘導する

    山田ひとみ, 山田ひとみ, 土門久哲, 宮内小百合, 宮内小百合, 宮沢春菜, 宮沢春菜, 中島貴子, 多部田康一, 山崎和久

    新潟歯学会雑誌   43 ( 2 )   2013年

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  • Porphyromonas gingivalis口腔感染マウスモデルで誘導されるインスリン抵抗性は脂肪組織及び肝臓における炎症反応と関連する

    有松圭, 土門久哲, 山田ひとみ, 宮内小百合, 宮沢春菜, 皆川高嘉, 中島麻由佳, 中島貴子, 多部田康一, 山崎和久

    日本歯周病学会学術大会プログラムおよび講演抄録集   56th   2013年

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▶ 全件表示

受賞

  • JADR・GC Young Investigator Award

    2012年12月   Japanese Association for Dental Research  

    宮沢春菜

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共同研究・競争的資金等の研究

  • PCSK9は歯周治療効果を評価する新たなバイオロジカルマーカーとなるか?

    研究課題/領域番号:19K18992  2019年4月 - 2022年3月

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    宮沢 春菜

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    配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

    血中コレステロールレベルを調節する分子であるProprotein convertase subtilisin/kexin type 9 (PCSK9)の血清レベルは,健常者と比較して歯周炎患者で高かった。PCSK9は歯周病検査による1歯単位の臨床指標とは別に,全身疾患に対するリスク評価も可能で,全身への影響を考慮した治療方針の選択に活用できる新たなバイオロジカルマーカーとなる可能性がある。本研究では,血清PCSK9レベルと歯周炎重症度,歯周治療前後での変動,他の臨床指標や炎症マーカーとの関連について解析し, 歯周治療効果を評価するPCSK9の有用性を明らかにする。

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  • スケーリング後菌血症に対する高齢者生体応答の解析と光治療による予防法の確立

    研究課題/領域番号:17K11984  2017年4月 - 2022年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    両角 俊哉, 高橋 直紀, 小松 康高, 保苅 崇大, 宮沢 春菜

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    配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

    あらゆる観血処置にともない,一過性の菌血症が発生する。我々はこれまでの一連の研究で,中年期の歯周炎患者において,1) スケーリング・ルートプレーニング(SRP)後に高頻度で菌血症が発生すること,2) 抗菌薬(アジスロマイシン)併用やエルビウムヤグ(Er: YAG)レーザー治療により菌血症の発生を減少または抑制できること,3) 局所薬物配送システム(LDDS)は機械的除去療法前の使用でも歯周ポケット内環境を改善できることなどを報告してきた。一方,免疫力が低下している高齢者においては,菌血症が一過性で終わらず,全身性の高リスクとなる可能性がある。そこで,高齢者に適した安全かつ効果的な歯周治療法を確立し,超高齢社会のニーズに応えるべく、本研究では慢性歯周炎を有する高齢者のSRP時に発生する菌血症,それにともなう生体応答の解析,および光治療(Er:YAGレーザー)による菌血症予防の有効性検討を目的とする。
    具体的には、① ハンドスケーラー群:ハンドスケーラーにてSRP実施 ② エルビウムヤグ(Er: YAG)レーザー群:レーザーにて処置 の2群間で、(1) 血清中炎症性メディエイター(高感度CRP、IL-6、IFN-g、TNF-a等) (2) ストレス指標(心拍変動、血中酸素飽和度等) (3) 臨床パラメーター の経時的変化および相関関係を比較解析し、予防対策について検討する。現在、2施設において研究進行中である。

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  • 歯周病原細菌は腸管透過性亢進に関与するか?

    2016年4月 - 2019年3月

    日本学術振興会  科学研究費 若手研究B 

    宮沢春菜

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    担当区分:研究代表者  資金種別:競争的資金

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  • 歯周病原細菌による腸管dysbiosisと動脈硬化との関連解明

    2014年4月 - 2016年3月

    日本学術振興会  科学研究費 研究活動スタート支援 

    宮沢春菜

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    担当区分:研究代表者  資金種別:競争的資金

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