Updated on 2022/12/05

写真a

 
TAKETSURU Hiroaki
 
Organization
Brain Research Institute Center for Bioresources Specially Appointed Assistant Professor
Title
Specially Appointed Assistant Professor
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Degree

  • 神戸大学(農学博士) ( 2011.3   神戸大学 )

Research Interests

  • Superovulation

  • In vitro fertilization

  • Oocyte

  • Vitrification

  • Embryo transfer

Research Areas

  • Life Science / Laboratory animal science  / Reproductive technology

Research History (researchmap)

  • Niigata University   Brain Research Institute Center for Bioresources   Specially Appointed Assistant Professor

    2021.10

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    Country:Japan

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  • Kyushu University   INAMORI Frontier Research Center   Assistant Professor (Specially appointed)

    2019.5 - 2021.9

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  • Interuniversity Bio-Backup Project for Basic Biology   A specially appointed substitute teacher

    2016.9 - 2019.4

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  • Institute of Laboratory Animals Graduate School of Medicine, Kyoto University   Specific researcher

    2011.4 - 2016.8

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Research History

  • Niigata University   Center for Bioresources, Brain Research Institute   Specially Appointed Assistant Professor

    2021.10

Education

  • Kobe University   Graduate School of Agricultural Science

    2008.4 - 2011.3

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Professional Memberships

  • 日本マーモセット研究会

    2021.11

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  • SOCIETY FOR REPRODUCTION AND DEVELOPMENT

    2014.5

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  • JAPANESE ASSOCIATION FOR LABORATORY ANIMAL SCIENCE

    2012.4

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Papers

  • Survivability and subsequent development of vitrified early-stage mouse embryos after warming at different temperatures Reviewed

    Hiroaki Taketsuru, Yu-ichi Tsukada, Takehito Kaneko

    Biochemical and Biophysical Research Communications   591   50 - 53   2022.2

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bbrc.2021.12.092

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  • Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage Reviewed

    Maemura M, Taketsuru H, Nakajima Y, Shao R, Kakihara A, Nogami J, Ohkawa Y, Tsukada Y

    Scientific Reports   2021

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    Language:English   Publishing type:Research paper (scientific journal)  

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  • Tolerance to vitrification of rat embryos at various developmental stages. Reviewed

    Taketsuru H, Kaneko T

    Cryobiology   84   1 - 3   2018.10

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  • In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability Reviewed

    Hiroaki Taketsuru, Takehito Kaneko

    Journal of Reproduction and Development   62 ( 5 )   521 - 526   2016

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Japanese Society of Animal Reproduction (JSAR)  

    Rat oocytes can be produced artificially by superovulation. Because some strains show low sensitivity to superovulation treatment, in vitro maturation is an alternative method to produce numerous matured oocytes. Furthermore, establishment of an in vitro maturation system with simple culture conditions is cost effective and leads to easy handling of oocytes. This study examined developmental ability of rat germinal vesicle (GV) oocytes maturing in vitro under simple culture conditions. Significantly different numbers of ovulated oocytes reached the second metaphase of meiosis (MII) among Jcl:Wistar (17.0), F344/Stm (31.0), and BN/SsNSlc (2.2) rats in whom superovulation was induced by pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin. However, similar numbers of GV oocytes were obtained from ovaries of PMSG-injected Wistar (27.7), F344 (34.7), and BN (24.7) rats. These GV oocytes were cultured in vitro in HTF, αMEM, and a 1:1 HTF + αMEM or TYH + αMEM mixture. High proportions of Wistar and F344 oocytes that matured to MII in αMEM were parthenogenetically activated by strontium chloride treatment (78% and 74%, respectively). Additionally, 10% of matured oocytes of both strains developed into offspring after intracytoplasmic sperm injection and embryo transfer to foster mothers. Although BN oocytes cultured in αMEM could be parthenogenetically activated and developed into offspring, the success rate was lower than that for Wistar and F344 oocytes. This study demonstrated that numerous GV oocytes were produced in rat ovaries by PMSG injection. This simple in vitro maturation system of immature oocytes could be further developed to maintain valuable rat strains experiencing reproductive difficulties.

    DOI: 10.1262/jrd.2016-057

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  • Efficient collection and cryopreservation of embryos in F344 strain inbred rats Reviewed

    Hiroaki Taketsuru, Takehito Kaneko

    CRYOBIOLOGY   67 ( 2 )   230 - 234   2013.10

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    In rats, it is now possible to produce genetically engineered strains, not only as transgenic animals but also using gene knockout techniques. Reproductive technologies have been used as indispensable tools to produce and maintain these novel valuable strains. Although studies for collecting and cryopreserving embryos have been reported using outbred rats, efficient methods have not been established in inbred strains. The F344 inbred strain is important in rat breeding and has been used for the production of transgenic/knockout strains and for genome sequencing. Here we studied the optimal conditions for oocyte collection by induction of superovulation, and the development of embryos after cryopreservation in F344 rats. The response to pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) was examined by injection of 150 IU/kg PMSG + 75 IU/kg hCG or 300 IU/kg PMSG + 300 IU/kg hCG. Superovulation was achieved at high efficiency by an injection of 150 IU/kg PMSG + 75 IU/kg hCG. Furthermore, superovulation in this strain showed similar high response as Wistar rats. Of 2-cell embryos cryopreserved by vitrification in a solution containing 10% propylene glycol, 30% ethylene glycol, 20% Percoll and 0.3 M sucrose, more than 90% survived after warming and 32% developed to offspring. However, the freezability of pronuclear stage embryos was extremely low. This study demonstrated that sufficient unfertilized oocytes and embryos can be collected from F344 rats by the induction of superovulation with 150 IU/kg PMSG + 75 IU/kg hCG. Furthermore, cryopreservation of 2-cell embryos using this vitrification protocol can now be applied to maintaining valuable rat strains derived from the F344 inbred strain as genetic resources. (C) 2013 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cryobiol.2013.07.004

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  • Effect of androstenedione on the growth and meiotic competence of bovine oocytes from early antral follicles Reviewed

    Hiroaki Taketsuru, Yuji Hirao, Naoki Takenouchi, Kosuke Iga, Takashi Miyano

    ZYGOTE   20 ( 4 )   407 - 415   2012.11

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:CAMBRIDGE UNIV PRESS  

    Medium that contains 17 beta-estradiol has been reported to support in vitro growth of bovine oocytes, isolated from early antral follicles, until the final stage. The aim of this study was to determine the effects of androstenedione in medium on such growing bovine oocytes. Oocyte-granulosa cell complexes were collected from early antral follicles and cultured for 14 days in medium supplemented with 17 beta-estradiol (0, 10 and 100 ng/ml) or androstenedione (0, 10 and 100 ng/ml). The mean diameter of oocytes measured after seeding on the culture substrate was 96.9 mu m (n = 191). Either steroid was necessary for maintainance of the organization of oocyte-granulosa cell complexes over the 14-day culture period. In the 17 beta-estradiol- or the androstenedione-supplemented medium about 80% or 65%, respectively, of viable oocytes were recovered. In both groups the increase in oocyte size was significant after 14 days. The in vitro grown oocytes were cultured for a further 22-24 h for oocyte maturation; 13% and 30% of oocytes grown in the 10 and 100 ng/ml 17 beta-estradiol-supplemented medium reached metaphase II, respectively; more than 64% of oocytes grown in the androstenedione-supplemented medium matured to metaphase II. These results show that androstenedione, as 17 beta-estradiol, can maintain the viability of bovine oocyte-granulosa cell complexes and support the growth of oocytes, and that androstenedione promotes the acquisition of oocyte meiotic competence efficiently at a low dose.

    DOI: 10.1017/S0967199411000268

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  • Bovine Oocytes in Secondary Follicles Grow in Medium Containing Bovine Plasma after Vitrification Reviewed

    Hiroaki Taketsuru, Asuka Takajo, Rong-Mei Bao, Atsushi Hamawaki, Motoichi Yoshikawa, Takashi Miyano

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   57 ( 1 )   99 - 106   2011.2

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOCIETY REPRODUCTION & DEVELOPMENT-SRD  

    There has been no culture system that supports the growth of bovine oocytes for more than 2 weeks. In the present study, bovine secondary follicles were cultured for 4 weeks, and the effects of supplemented protein components and FSH in the culture medium on the growth of the oocytes were examined. The effect of vitrification of secondary follicles on the subsequent oocyte growth was also examined. Secondary follicles (150 to 200 mu m in diameter) containing growing oocytes (approximately 60 mu m in diameter) were dissected from ovaries and cultured in a medium supplemented with FSH (0, 25 or 50 ng/ml) and one of the following four kinds of protein components: bovine serum albumin (BSA), bovine plasma (BPL), fetal calf serum (FCS) and bovine follicular fluid (BFF). In BSA- and BPL-supplemented media with 0 or 25 ng/ml FSH, more than 50% of follicles showed no degenerative signs during culture, and oocytes significantly increased in size after 4 weeks (P<0.05). Higher percentages of granulosa cell-enclosed oocytes were recovered from the follicles cultured in BPL-supplemented media with 0 and 25 ng/ml FSH, and the oocytes grew to 90 mu m or more in diameter. In FCS- and BFF-supplemented media, FSH increased the numbers of degenerating follicles. Next, vitrified-warmed secondary follicles were cultured in BPL-supplemented medium. One third of the follicles showed no degenerative signs, and the oocytes increased in diameter to 88.8 +/- 3.1 mu m after 4 weeks of culture. These results suggest that a BPL-supplemented medium supports oocyte growth in bovine secondary follicles for 4 weeks, even after vitrification and warming of the follicles.

    DOI: 10.1262/jrd.10-047H

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  • FOXO3 knockdown accelerates development of bovine primordial follicles Reviewed

    Rong-Mei Bao, Kohei Hayakawa, Mohammad Moniruzzaman, Hiroaki Taketsuru, Takashi Miyano

    Journal of Reproduction and Development   57 ( 4 )   475 - 480   2011

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    Language:English   Publishing type:Research paper (scientific journal)  

    The objective of the present study was to elucidate the involvement of FOXO3 in the activation of bovine primordial follicles. In immunohistochemistry, FOXO3 was detected in all of the oocytes in primordial and primary follicles. The FOXO3 decreased after treatment with FOXO3 small interfering RNAs (siRNAs). Ovarian tissues containing dominantly primordial follicles were treated with FOXO3 siRNAs and then xenografted to severe combined immune deficiency (SCID) mice. Two months after xenografting, some primordial follicles developed to the secondary and tertiary stages, and the total percentage of these developing follicles (secondary and tertiary follicles: 18 ± 7%) was higher than in the control grafts treated with control siRNA (7 ± 1%). It is thought that bovine primordial follicle activation is regulated by the FOXO3-dependent mechanism and that knockdown of FOXO3 induces the release of primordial follicles from FOXO3 suppression, initiating their growth. © 2011 by the Society for Reproduction and Development.

    DOI: 10.1262/jrd.11-013H

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  • Histological and biological assessment of vitrified ovarian follicles from large animals Reviewed

    Rong-Mei Bao, Hiroaki Taketsuru, Takashi Miyano

    Reproductive Medicine and Biology   10 ( 4 )   211 - 219   2011

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    Language:English   Publisher:John Wiley and Sons Ltd  

    Mammalian ovaries contain mixed populations of follicles at different developmental stages. A combination of vitrification and growth culture of ovarian follicles could provide the desired number of mature eggs from a preserved small amount of ovarian tissues. Secondary and primordial follicles from porcine and bovine ovaries were vitrified in solutions containing ethylene glycol, dimethyl sulfoxide and different concentrations of sucrose, and assessed via histological examination, viability staining, xenografting to immunodeficient mice, and in vitro culturing. Histological examination revealed the damage to oocytes and the damage to follicle components separately. The effects of sucrose in vitrification solutions on the follicles were different depending on the developmental stage of the follicle, oocyte size, cell type in the follicle, and species. Viability staining with fluorescein diacetate was useful to assess the damage to oocytes in secondary follicles. In the xenografts, vitrified bovine primordial and secondary follicles developed to the antral stage, and vitrified porcine primordial follicles developed to the secondary stage. Furthermore, bovine secondary follicles formed antrum-like structures in culture. These results suggest that histological examination and viability staining are valuable for assessing the direct effects of vitrification and warming conditions on follicles and oocytes, while xenografting and in vitro culturing can be useful for evaluating the developmental ability of vitrified follicles and oocytes. © Japan Society for Reproductive Medicine 2011.

    DOI: 10.1007/s12522-011-0094-5

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  • Development of vitrified porcine primordial follicles in xenografts Reviewed

    M. Moniruzzaman, R. -M. Bao, H. Taketsuru, T. Miyano

    THERIOGENOLOGY   72 ( 2 )   280 - 288   2009.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE INC  

    The objective was to cryopreserve porcine primordial follicles by vitrification and to assess the development of these follicles in xenografts. Ovarian tissues containing primordial follicles were collected from neonatal (15-d-old) piglets. They were vitrified in modified tissue culture medium (TCM)-199 containing 15% (v/v) ethylene glycol, 15% (v/v) dimethylsulfoxide, 20% (v/v) fetal calf serum, and 0, 0.25, or 0.5 M sucrose. After 1 wk of storage in liquid nitrogen (LN(2)), the tissues were warmed, and the morphology of follicles and oocytes was examined histologically. After vitrification in sucrose-free medium, there were 50 +/- 2 (mean +/- SEM; n = 10) follicles per tissue, in contrast with 108 +/- 10 (n = 10) in fresh tissues. Losses were attributed to puncturing oocytes during the vitrification-warming process, as oocytes were apparently normal after treatment of the sucrose-free vitrification solution without plunging into LN(2). When tissues were vitrified in sucrose-supplemented medium, loss of oocytes decreased (P < 0.05). However, the number of abnormal oocytes having nuclear shrinkage was increased (P < 0.05) by the addition of 0.5 M sucrose; this occurred in a small number of oocytes treated With sucrose-supplemented vitrification Solutions without vitrification. After 2 mo of xenografting, of vitrified-warmed tissues in SCID (severe combined immune deficiency) mice, primordial follicles developed to the secondary stage (accompanied by oocyte growth), whereas there was development to the antral stage in xenografts of fresh tissues. In conclusion, primordial follicles from neonatal pigs maintained their developmental ability after vitrification and warming, although their developmental rate was slower than that of the fresh control in xenografts. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.theriogenology.2009.01.024

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Presentations

  • ラットGV期卵の体外成熟に及ぼす顆粒膜細胞の影響

    竹鶴 裕亮, 平山 瑠那, 夏目 里恵, 阿部 学, 﨑村 建司, 笹岡 俊邦, 金子 武人

    第69回日本実験動物学会総会  2022.5 

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    Event date: 2022.5

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  • マーモセット卵巣異種間移植による受精卵の生産 Invited

    竹鶴 裕亮, 平山 瑠那, 中務 胞, 夏目 里恵, 村田 康輔, 齊藤奈英, 足立 周子, 桑原 沙耶香, 平澤 克哉, 阿部 学, 﨑村 建司, 笹岡 俊邦

    マーモセットミーティング 

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    Event date: 2022.4

    Presentation type:Oral presentation (invited, special)  

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  • マウス初期胚のガラス化保存後の融解速度がその後の発生に及ぼす影響

    竹鶴 裕亮, 束田 裕一, 金子 武人

    第68回日本実験動物学会総会 

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    Event date: 2021.5

    Language:Japanese   Presentation type:Poster presentation  

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  • 全能性とは? Invited

    竹鶴 裕亮

    第2回オンラインポスターセッション  2021.3 

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • ラット胚の発生ステージがガラス化保存後の発生に及ぼす影響

    金子 武人, 竹鶴 裕亮

    第112回日本繁殖生物学会大会  2019.9 

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  • ラット前核期胚の発達時期がガラス化保存後の発生に及ぼす影響

    竹鶴 裕亮, 金子 武人

    第66回日本実験動物学会総会  2019.5 

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  • ラット前核期胚の発達時期がガラス化保存後の発生に及ぼす影響

    竹鶴 裕亮, 金子 武人

    Cryopreservation Conference 2018  2018.10 

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  • ラット前核期胚の発達時期がガラス化保存後の発生に及ぼす影響

    竹鶴 裕亮, 金子 武人

    第111回日本繁殖生物学会大会  2018.9 

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  • ラット系統別の排卵数と凍結保存後の胚発生

    竹鶴 裕亮, 金子 武人

    Cryopreservation Conference 2017  2017.11 

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  • Development of rat vitrified embryos in each developmental stage

    Taketsuru H, Kaneko T

    Fourth World Congress of Reproductive Biology (WCRB 2017)  2017.9 

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  • Interuniversity Bio-Backup Project (IBBP) for Basic Biology

    Taketsuru H, Kato A, Matsubayashi N, Mizokami Y, Tsuzuki C, Naruse K

    International Society for Biological and Environmental Repositories (ISBER) 2017  2017.5 

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  • ラット胚のステージの違いによる凍結保存後の産仔発生への影響

    竹鶴 裕亮, 金子 武人

    Cryopreservation Conference 2016  2016.11 

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  • ラット卵母細胞の体外成熟およびその後の発生能について

    竹鶴 裕亮, 金子 武人

    第109回日本繁殖生物学会大会  2016.9 

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  • ラット前核期胚の凍結保存および移植後の産子への発生について

    竹鶴 裕亮, 金子 武人

    第63回日本実験動物学会総会  2016.5 

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  • ラット未成熟卵母細胞の体外成熟法の検討

    竹鶴 裕亮, 金子 武人

    第62回日本実験動物学会総会  2015.5 

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  • ラット胚の凍結保存および融解後の産子への発生について

    竹鶴 裕亮, 金子 武人

    第124回関西実験動物研究会  2014.12 

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  • ラット前核期胚のガラス化保存法の検討

    竹鶴 裕亮, 金子 武人

    第107回日本繁殖生物学会  2014.8 

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  • ラット初期胚の凍結保存および融解後の胚の発生について

    竹鶴 裕亮, 金子 武人

    第61回日本実験動物学会総会  2014.5 

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  • ナショナルバイオリソースプロジェクト「ラット」(NBRP-Rat)で保存されている凍結胚および凍結精子からの産子作出成績

    金子 武人, 竹鶴 裕亮, 真下 知士, 庫本 高志

    第48回日本実験動物技術者協会総会  2014.5 

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  • ナショナルバイオリソースプロジェクト「ラット」(NBRP-Rat)で保存されている凍結胚の品質評価

    竹鶴 裕亮, 真下 知士, 庫本 高志, 芹川 忠夫, 金子 武人

    第60回日本実験動物学会総会  2013.5 

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  • 過排卵処理によるF344/Stmラット2細胞期胚の効率的採取

    竹鶴 裕亮, 芹川 忠夫, 金子 武人

    第59回日本実験動物学会総会  2012.5 

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  • F344/Stmラットにおける効率的な卵子および胚採取法の検討

    竹鶴 裕亮, 芹川 忠夫, 金子 武人

    第112回関西実験動物研究会  2011.12 

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  • 初期胞状卵胞から分離したウシ卵母細胞の体外発育に及ぼすアンドロステンジオンの影響

    竹鶴 裕亮, 平尾 雄二, 竹之内 直樹, 伊賀 浩輔, 宮野 隆

    日本畜産学会第112回大会  2010.3 

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  • Bovine oocytes in secondary follicles grow in the medium containing bovine plasma after vitrification

    Taketsuru H, Bao RM, Takajo A, Hamawaki A, Yoshikawa M, Miyano T

    Japan-Taiwan Joint Symposium on Cell Signaling and Gene Regulation  2009.11 

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  • ウシ二次卵胞のガラス化保存と融解後の卵母細胞の発育

    竹鶴 裕亮, 高城 明日香, 浜脇 淳, 吉川 基一, 宮野 隆

    第134回日本生殖医学学会関西支部集談会 第38回関西アンドロロジーカンファレンス  2009.3 

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  • ガラス化したウシ二次卵胞の体外培養による卵母細胞の発育

    竹鶴 裕亮, 高城 明日香, 浜脇 淳, 吉川 基一, 宮野 隆

    日本畜産学会第109回大会  2008.3 

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Research Projects

  • 哺乳類全能性(totipotent)細胞の分離法の開発

    2020 - 2021

    九州大学  QRプログラム(わかばチャレンジ) 

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    Authorship:Principal investigator 

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  • ラット未受精卵および初期胚における凍結保存法の開発

    2018 - 2019

    基礎生物学研究所  共同利用研究 

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    Authorship:Coinvestigator(s) 

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  • 過排卵が難しい疾患モデル動物の系統保存に向けたその原因因子の探索と胚保存法の開発

    2016.4 - 2018.3

    科学研究費  若手研究(B) 

    竹鶴 裕亮

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    Authorship:Principal investigator  Grant type:Competitive

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Teaching Experience (researchmap)

  • 基礎生物学概論II

    Institution name:基礎生物学研究所

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Social Activities

  • IBBP ラット生殖技術講習会 2018

    Role(s): Lecturer

    2018.12

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  • IBBP ラット生殖技術講習会 2017

    Role(s): Lecturer

    2017.12

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