Updated on 2025/01/01

写真a

 
OTOGURO Teruhime
 
Organization
Academic Assembly Institute of Medicine and Dentistry Specially Appointed Assistant Professor
Graduate School of Medical and Dental Sciences Specially Appointed Assistant Professor
Title
Specially Appointed Assistant Professor
External link

Degree

  • 博士(医科学) ( 2020.3   山梨大学 )

Research History (researchmap)

  • Niigata University   Graduate School of Medical and Dental Sciences   Specially Appointed Assistant Professor

    2022.4

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  • 一般社団法人予防衛生協会   試験検査部   研究員

    2020.6 - 2022.3

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Research History

  • Niigata University   Graduate School of Medical and Dental Sciences   Specially Appointed Assistant Professor

    2022.4

  • Niigata University   Institute of Medicine and Dentistry, Academic Assembly   Specially Appointed Assistant Professor

    2022.4

 

Papers

  • Changing Seasonality of Influenza in the Post-COVID Era in Japan.

    Keita Wagatsuma, Teruhime Otoguro

    JMA journal   7 ( 1 )   138 - 139   2024.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.31662/jmaj.2023-0150

    PubMed

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  • Molecular Epidemiology of Respiratory Syncytial Virus during 2019-2022 and Surviving Genotypes after the COVID-19 Pandemic in Japan. International journal

    Sayaka Yoshioka, Wint Wint Phyu, Keita Wagatsuma, Takao Nagai, Yasuko Sano, Kiyosu Taniguchi, Nobuo Nagata, Kazuhiko Tomimoto, Isamu Sato, Harumi Kaji, Ken Sugata, Katsumi Sugiura, Naruo Saito, Satoshi Aoki, Eitaro Suzuki, Yasushi Shimada, Hirotsune Hamabata, Irina Chon, Teruhime Otoguro, Hisami Watanabe, Reiko Saito

    Viruses   15 ( 12 )   2023.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    To evaluate the changes in respiratory syncytial virus (RSV) collected between 2019 and 2022, we analyzed RSV-A and RSV-B strains from various prefectures in Japan before and after the COVID-19 pandemic. RT-PCR-positive samples collected from children with rapid test positivity at outpatient clinics in 11 prefectures in Japan were sequenced for the ectodomain of the G gene to determine the genotype. Time-aware phylogeographic analyses were performed using the second hypervariable region (HVR) of the G gene from 2012 to 2022. Of 967 samples, 739 (76.4%) were found to be RSV-positive using RT-PCR. RSV peaked in September 2019 but was not detected in 2020, except in Okinawa. Nationwide epidemics occurred with peaks in July 2021 and 2022. The genotype remained the same, ON1 for RSV-A and BA9 for RSV-B during 2019-2022. Phylogeographic analysis of HVR revealed that at least seven clusters of RSV-A had circulated previously but decreased to two clusters after the pandemic, whereas RSV-B had a single monophyletic cluster over the 10 years. Both RSV-A and RSV-B were transferred from Okinawa into other prefectures after the pandemic. The RSV epidemic was suppressed due to pandemic restrictions; however, pre-pandemic genotypes spread nationwide after the pandemic.

    DOI: 10.3390/v15122382

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  • Whole-Genome Analysis of Influenza A(H3N2) and B/Victoria Viruses Detected in Myanmar during the COVID-19 Pandemic in 2021. International journal

    Irina Chon, Reiko Saito, Yadanar Kyaw, Moe Myat Aye, Swe Setk, Wint Wint Phyu, Keita Wagatsuma, Jiaming Li, Yuyang Sun, Teruhime Otoguro, Su Mon Kyaw Win, Sayaka Yoshioka, Nay Chi Win, Lasham Di Ja, Htay Htay Tin, Hisami Watanabe

    Viruses   15 ( 2 )   2023.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    An influenza circulation was observed in Myanmar between October and November in 2021. Patients with symptoms of influenza-like illness were screened using rapid diagnostic test (RDT) kits, and 147/414 (35.5%) upper respiratory tract specimens presented positive results. All RDT-positive samples were screened by a commercial multiplex real-time polymerase chain reaction (RT-PCR) assay, and 30 samples positive for influenza A(H3N2) or B underwent further typing/subtyping for cycle threshold (Ct) value determination based on cycling probe RT-PCR. The majority of subtyped samples (n = 13) were influenza A(H3N2), while only three were B/Victoria. Clinical samples with low Ct values obtained by RT-PCR were used for whole-genome sequencing via next-generation sequencing technology. All collected viruses were distinct from the Southern Hemisphere vaccine strains of the corresponding season but matched with vaccines of the following season. Influenza A(H3N2) strains from Myanmar belonged to clade 2a.3 and shared the highest genetic proximity with Bahraini strains. B/Victoria viruses belonged to clade V1A.3a.2 and were genetically similar to Bangladeshi strains. This study highlights the importance of performing influenza virus surveillance with genetic characterization of the influenza virus in Myanmar, to contribute to global influenza surveillance during the COVID-19 pandemic.

    DOI: 10.3390/v15020583

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  • Establishment of a Cell Culture Model Permissive for Infection by Hepatitis B and C Viruses Reviewed

    Teruhime Otoguro, Tomohisa Tanaka, Hirotake Kasai, Nobuhiro Kobayashi, Atsuya Yamashita, Takasuke Fukuhara, Akihide Ryo, Moto Fukai, Akinobu Taketomi, Yoshiharu Matsuura, Kohji Moriishi

    Hepatology Communications   5 ( 4 )   634 - 649   2021.4

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    Compared with each monoinfection, coinfection with hepatitis B virus (HBV) and hepatitis C virus (HCV) is well known to increase the risks of developing liver cirrhosis and hepatocellular carcinoma. However, the mechanism by which HBV/HCV coinfection is established in hepatocytes is not well understood. Common cell culture models for coinfection are required to examine viral propagation. In this study, we aimed to establish a cell line permissive for both HBV and HCV infection. We first prepared a HepG2 cell line expressing sodium taurocholate cotransporting polypeptide, an HBV receptor, and then selected a cell line highly permissive for HBV infection, G2/NT18-B. After transduction with a lentivirus-encoding microRNA-122, the cell line harboring the highest level of replicon RNA was selected and then treated with anti-HCV compounds to eliminate the replicon RNA. The resulting cured cell line was transduced with a plasmid-encoding CD81. The cell line permissive for HCV infection was cloned and then designated the G2BC-C2 cell line, which exhibited permissiveness for HBV and HCV propagation. JAK inhibitor I potentiated the HCV superinfection of HBV-infected cells, and fluorescence-activated cell-sorting analysis indicated that HBV/HCV double-positive cells accounted for approximately 30% of the coinfected cells. Among several host genes tested, cyclooxygenase-2 showed synergistic induction by coinfection compared with each monoinfection. Conclusion: These data indicate that our in vitro HBV/HCV coinfection system provides an easy-to-use platform for the study of host and viral responses against coinfection and the development of antiviral agents targeting HBV and HCV.

    DOI: 10.1002/hep4.1653

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/hep4.1653

  • Roles of the 5' Untranslated Region of Nonprimate Hepacivirus in Translation Initiation and Viral Replication. International journal

    Tomohisa Tanaka, Teruhime Otoguro, Atsuya Yamashita, Hirotake Kasai, Takasuke Fukuhara, Yoshiharu Matsuura, Kohji Moriishi

    Journal of virology   92 ( 7 )   2018.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    The 5' untranslated region (UTR) of hepatitis C virus (HCV), which is composed of four domains (I, II, III, and IV) and a pseudoknot, is essential for translation and viral replication. Equine nonprimate hepacivirus (EHcV) harbors a 5' UTR consisting of a large 5'-terminal domain (I); three additional domains (I', II, and III), which are homologous to domains I, II, and III, respectively, of HCV; and a pseudoknot, in the order listed. In this study, we investigated the roles of the EHcV 5' UTR in translation and viral replication. The internal ribosome entry site (IRES) activity of the EHcV 5' UTR was lower than that of the HCV 5' UTR in several cell lines due to structural differences in domain III. Domains I and III of EHcV were functional in the HCV 5' UTR in terms of IRES activity and the replication of the subgenomic replicon (SGR), although domain II was not exchangeable between EHcV and HCV for SGR replication. Furthermore, the region spanning domains I and I' of EHcV (the 5'-proximal EHcV-specific region) improved RNA stability and provided the HCV SGR with microRNA 122 (miR-122)-independent replication capability, while EHcV domain I alone improved SGR replication and RNA stability irrespective of miR-122. These data suggest that the region spanning EHcV domains I and I' improves RNA stability and viral replication regardless of miR-122 expression. The 5'-proximal EHcV-specific region may represent an inherent mechanism to facilitate viral replication in nonhepatic tissues.IMPORTANCE EHcV is the closest viral homolog to HCV among other hepaciviruses. HCV exhibits a narrow host range and liver-specific tropism, while epidemiological reports suggest that EHcV infects the liver and respiratory organs in horses, donkeys, and dogs. However, the mechanism explaining the differences in host or organ tropism between HCV and EHcV is unknown. In this study, our data suggest that the 5' untranslated region (UTR) of EHcV is composed of an internal ribosome entry site (IRES) element that is functionally exchangeable with HCV IRES elements. Furthermore, the 5'-proximal EHcV-specific region enhances viral replication and RNA stability in a miR-122-independent manner. Our data suggest that the region upstream of domain II in the EHcV 5' UTR contributes to the differences in tissue tropism observed between these hepaciviruses.

    DOI: 10.1128/JVI.01997-17

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MISC

  • Changes in transmission dynamics of respiratory infections during the COVID-19 pandemic

    Otoguro Teruhime, Wagatsuma Keita

    Kokusai Hoken Iryo (Journal of International Health)   39 ( 1 )   15 - 17   2024

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    Language:Japanese   Publisher:JAPAN ASSOCIATION FOR INTERNATIONAL HEALTH  

    DOI: 10.11197/jaih.39.15

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