2022/10/13 更新

写真a

オオツカ タカヨシ
大塚 尭慶
OTSUKA Takayoshi
所属
脳研究所 生命科学リソース研究センター 助教
職名
助教
外部リンク

学位

  • 博士(理学) ( 2016年3月   東京大学 )

研究キーワード

  • 間葉系幹細胞

  • 器官形成

  • 再生

  • 発生生物学

研究分野

  • ライフサイエンス / 発生生物学

経歴(researchmap)

  • 新潟大学   脳研究所附属生命科学リソース研究センター   助教

    2022年7月

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    国名:日本国

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  • UConn Health Center   Connecticut Convergence Institute for Translation in Regenerative Engineering   Post doctoral fellow

    2018年1月 - 2022年6月

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  • University of California, Irvine   Department of Developmental and Cell Biology   Post doctoral fellow

    2016年4月 - 2017年12月

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経歴

  • 新潟大学   脳研究所 生命科学リソース研究センター   助教

    2022年7月 - 現在

学歴

  • 東京大学   理学系研究科   生物科学専攻(博士課程)

    2013年4月 - 2016年3月

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  • 東京大学   理学系研究科   生物科学専攻(修士課程)

    2011年4月 - 2013年3月

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  • 東京大学   理学部   生物学科動物学コース

    2007年4月 - 2011年3月

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論文

  • Muscle degeneration in chronic massive rotator cuff tears of the shoulder: Addressing the real problem using a graphene matrix 査読

    Nikoo Saveh Shemshaki, Ho-Man Kan, Mohammed Barajaa, Takayoshi Otsuka, Amir Lebaschi, Neha Mishra, Lakshmi S. Nair, Cato T. Laurencin

    Proceedings of the National Academy of Sciences   2022年8月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1073/pnas.2208106119

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  • Overexpression of NDST1 attenuates fibrotic response in murine adipose-derived stem cells 査読

    Takayoshi Otsuka, Ho-Man Kan, Timothy D Mason, Lakshmi S Nair, Cato T Laurencin

    Stem Cells and Development   2022年8月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Mary Ann Liebert Inc  

    DOI: 10.1089/scd.2022.0053

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  • Regenerative Engineering Approaches to Scar-Free Skin Regeneration 査読

    Takayoshi Otsuka, Ho-Man Kan, Cato T. Laurencin

    Regenerative Engineering and Translational Medicine   8 ( 2 )   225 - 247   2022年6月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s40883-021-00229-8

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    その他リンク: https://link.springer.com/article/10.1007/s40883-021-00229-8/fulltext.html

  • Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment 査読

    Maumita Bhattacharjee, Jorge L. Escobar Ivirico, Ho-Man Kan, Shiv Shah, Takayoshi Otsuka, Rosalie Bordett, Mohammed Barajaa, Naveen Nagiah, Rishikesh Pandey, Lakshmi S. Nair, Cato T. Laurencin

    Proceedings of the National Academy of Sciences   2022年1月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1073/pnas.2120968119

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  • Minimally Invasive Cellular Therapies for Osteoarthritis Treatment 査読

    Shiv Shah, Takayoshi Otsuka, Maumita Bhattacharjee, Cato T. Laurencin

    Regenerative Engineering and Translational Medicine   7 ( 1 )   76 - 90   2021年3月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s40883-020-00184-w

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    その他リンク: http://link.springer.com/article/10.1007/s40883-020-00184-w/fulltext.html

  • Control of mesenchymal cell fate via application of FGF-8b in vitro 査読

    Takayoshi Otsuka, Paulos Y. Mengsteab, Cato T. Laurencin

    Stem Cell Research   51   102155 - 102155   2021年3月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.scr.2021.102155

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  • Preparation and characterization of amnion hydrogel and its synergistic effect with adipose derived stem cells towards IL1β activated chondrocytes 査読

    Maumita Bhattacharjee, Jorge L. Escobar Ivirico, Ho-Man Kan, Rosalie Bordett, Rishikesh Pandey, Takayoshi Otsuka, Lakshmi S. Nair, Cato T. Laurencin

    Scientific Reports   10 ( 1 )   2020年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    <title>Abstract</title>
    Inflammation leads to chondrocyte senescence and cartilage degeneration, resulting in osteoarthritis (OA). Adipose‐derived stem cells (ADSCs) exert paracrine effects protecting chondrocytes from degenerative changes. However, the lack of optimum delivery systems for ADSCs limits its use in the clinic. The use of extracellular matrix based injectable hydrogels has gained increased attention due to their unique properties. In the present study, we developed hydrogels from amnion tissue as a delivery system for ADSCs. We investigated the potential of amnion hydrogel to maintain ADSC functions, the synergistic effect of AM with ADSC in preventing the catabolic responses of inflammation in stimulated chondrocytes. We also investigated the role of Wnt/β-catenin signaling pathway in IL-1β induced inflammation in chondrocytes and the ability of AM-ADSC to inhibit Wnt/β-catenin signaling. Our results showed that AM hydrogels supported cell viability, proliferation, and stemness. ADSCs, AM hydrogels and AM-ADSCs inhibited the catabolic responses of IL-1β and inhibited the Wnt/β-catenin signaling pathway, indicating possible involvement of Wnt/β-catenin signaling pathways in IL-1β induced inflammation. The results also showed that the synergistic effect of AM-ADSCs was more pronounced in preventing catabolic responses in activated chondrocytes. In conclusion, we showed that AM hydrogels can be used as a potential carrier for ADSCs, and can be developed as a potential therapeutic agent for treating OA.

    DOI: 10.1038/s41598-020-75921-w

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    その他リンク: http://www.nature.com/articles/s41598-020-75921-w

  • Mechanically superior matrices promote osteointegration and regeneration of anterior cruciate ligament tissue in rabbits 査読

    Paulos Y. Mengsteab, Takayoshi Otsuka, Aneesah McClinton, Nikoo Saveh Shemshaki, Shiv Shah, Ho-Man Kan, Elifho Obopilwe, Anthony T. Vella, Lakshmi S. Nair, Cato T. Laurencin

    Proceedings of the National Academy of Sciences   117 ( 46 )   28655 - 28666   2020年11月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Proceedings of the National Academy of Sciences  

    The gold standard treatment for anterior cruciate ligament (ACL) reconstruction is the use of tendon autografts and allografts. Limiting factors for this treatment include donor site morbidity, potential disease transmission, and variable graft quality. To address these limitations, we previously developed an off-the-shelf alternative, a poly(<sc>l</sc>-lactic) acid (PLLA) bioengineered ACL matrix, and demonstrated its feasibility to regenerate ACL tissue. This study aims to 1) accelerate the rate of regeneration using the bioengineered ACL matrix by supplementation with bone marrow aspirate concentrate (BMAC) and growth factors (BMP-2, FGF-2, and FGF-8) and 2) increase matrix strength retention. Histological evaluation showed robust tissue regeneration in all groups. The presence of cuboidal cells reminiscent of ACL fibroblasts and chondrocytes surrounded by an extracellular matrix rich in anionic macromolecules was up-regulated in the BMAC group. This was not observed in previous studies and is indicative of enhanced regeneration. Additionally, intraarticular treatment with FGF-2 and FGF-8 was found to suppress joint inflammation. To increase matrix strength retention, we incorporated nondegradable fibers, polyethylene terephthalate (PET), into the PLLA bioengineered ACL matrix to fabricate a “tiger graft.” The tiger graft demonstrated the greatest peak loads among the experimental groups and the highest to date in a rabbit model. Moreover, the tiger graft showed superior osteointegration, making it an ideal bioengineered ACL matrix. The results of this study illustrate the beneficial effect bioactive factors and PET incorporation have on ACL regeneration and signal a promising step toward the clinical translation of a functional bioengineered ACL matrix.

    DOI: 10.1073/pnas.2012347117

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    その他リンク: https://syndication.highwire.org/content/doi/10.1073/pnas.2012347117

  • Identification of Heparan-Sulfate Rich Cells in the Loose Connective Tissues of the Axolotl (Ambystoma mexicanum) with the Potential to Mediate Growth Factor Signaling during Regeneration 査読

    T. Otsuka, A. Q. Phan, C. T. Laurencin, J. D. Esko, S. V. Bryant, D. M. Gardiner

    Regenerative Engineering and Translational Medicine   6 ( 1 )   7 - 17   2020年3月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s40883-019-00140-3

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    その他リンク: http://link.springer.com/article/10.1007/s40883-019-00140-3/fulltext.html

  • Evaluation of a bioengineered ACL matrix’s osteointegration with BMP-2 supplementation 査読

    Paulos Y. Mengsteab, Patrick Conroy, Mary Badon, Takayoshi Otsuka, Ho-Man Kan, Anthony T. Vella, Lakshmi S. Nair, Cato T. Laurencin

    PLOS ONE   15 ( 1 )   e0227181 - e0227181   2020年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    DOI: 10.1371/journal.pone.0227181

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  • The Axolotl Limb Regeneration Model as a Discovery Tool for Engineering the Stem Cell Niche 査読

    Negar Seyedhassantehrani, Takayoshi Otsuka, Shambhavi Singh, David M. Gardiner

    Current Stem Cell Reports   3 ( 3 )   156 - 163   2017年9月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s40778-017-0085-5

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    その他リンク: http://link.springer.com/content/pdf/10.1007/s40778-017-0085-5.pdf

  • Targeted Ablation of Pancreatic β Cells in Medaka 査読

    Otsuka Takayoshi, Takeda Hiroyuki

    Zoological science   34 ( 3 )   179 - 184   2017年6月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Zoological Society of Japan  

    DOI: 10.2108/zs170004

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    CiNii Article

    CiNii Books

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    その他リンク: http://www.bioone.org/doi/pdf/10.2108/zs170004

  • Development of the pancreas in medaka,Oryzias latipes, from embryo to adult 査読

    Takayoshi Otsuka, Tatsuya Tsukahara, Hiroyuki Takeda

    Development, Growth & Differentiation   57 ( 8 )   557 - 569   2015年10月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/dgd.12237

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  • Large hypomethylated domains serve as strong repressive machinery for key developmental genes in vertebrates 査読 国際誌

    Ryohei Nakamura, Tatsuya Tsukahara, Wei Qu, Kazuki Ichikawa, Takayoshi Otsuka, Katsumi Ogoshi, Taro L. Saito, Kouji Matsushima, Sumio Sugano, Shinichi Hashimoto, Yutaka Suzuki, Shinichi Morishita, Hiroyuki Takeda

    Development   141 ( 13 )   2568 - 2580   2014年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:The Company of Biologists  

    DNA methylation is a fundamental epigenetic modification in vertebrate genomes and a small fraction of genomic regions is hypomethylated. Previous studies have implicated hypomethylated regions in gene regulation, but their functions in vertebrate development remain elusive. To address this issue, we generated epigenomic profiles that include base-resolution DNA methylomes and histone modification maps from both pluripotent cells and mature organs of medaka fish and compared the profiles with those of human ES cells. We found that a subset of hypomethylated domains harbor H3K27me3 (K27HMDs) and their size positively correlates with the accumulation of H3K27me3. Large K27HMDs are conserved between medaka and human pluripotent cells and predominantly contain promoters of developmental transcription factor genes. These key genes were found to be under strong transcriptional repression, when compared with other developmental genes with smaller K27HMDs. Furthermore, human-specific K27HMDs show an enrichment of neuronal activity-related genes, which suggests a distinct regulation of these genes in medaka and human. In mature organs, some of the large HMDs become shortened by elevated DNA methylation and associate with sustained gene expression. This study highlights the significance of domain size in epigenetic gene regulation. We propose that large K27HMDs play a crucial role in pluripotent cells by strictly repressing key developmental genes, whereas their shortening consolidates long-term gene expression in adult differentiated cells.

    DOI: 10.1242/dev.108548

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