記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Mary Ann Liebert Inc  

Artificial dermis (AD) has been used to regenerate dermis-like tissues in the treatment of full-thickness skin defects, but it takes 2 or 3 weeks to complete dermal regeneration. Our previous study demonstrated that injection of basic fibroblast growth factor (bFGF)-impregnated gelatin microspheres (MS) into the AD accelerates the regeneration of dermis-like tissue. However, injection of gelatin MS before clinical use is complicated and time consuming. This study investigated a new scaffold, in which collagen and gelatin are integrated, and which is capable of sustained bFGF release. We produced collagen/gelatin sponges with a gelatin concentration of 0wt%, 10wt%, 30wt%, and 50wt%. The mean pore size in each sponge decreased with the gelatin concentration. In an in vitro study, proliferation of fibroblasts in each sponge was not significantly different over 7 days of culture. As for in vivo sustained release of bFGF, a radioisotope study demonstrated that retention of bFGF in gelatin 10wt% and 30wt% sponges was significantly larger than that in gelatin 0wt% sponge. The collagen/gelatin sponges were grafted on full-thickness skin defects created on a rabbit ear, and we evaluated regeneration of dermis-like tissue by measuring the amount of hemoglobin and size of dermis-like tissue on histological sections. Seven days after implantation, the amount of hemoglobin in dermis-like tissue in gelatin 10wt% sponge was significantly larger than those in control and gelatin 50wt% sponge. Twenty-eight days after implantation, the area of dermis-like tissue in gelatin 10wt% sponge was significantly larger than those in the other specimens. We conclude that the collagen sponge integrated with 10wt% gelatin has the most potential for sustained release of bFGF and that the combination of collagen/gelatin 10wt% sponge and bFGF is a promising therapeutic modality for the treatment of full-thickness skin defects.

DOI： 10.1089/ten.tea.2007.0215

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その他リンク： https://www.liebertpub.com/doi/pdf/10.1089/ten.tea.2007.0215

記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND: Skin grafting is an important procedure to cover skin defects. Recently, cultured epidermal sheets and bilayered cultured skin have been used clinically, but they lack subcutaneous tissue. The objective of this study was to produce a bilayered dermal substitute with adipose tissue simultaneously in vivo. MATERIALS AND METHODS: We disseminated adipo-stromal cells on one side of a collagen sponge at a density of 1,0 x 10(5)cells/cm(2) and incubated overnight. Then, we turned over the sponge and disseminated dermal fibroblasts and keratinocytes at a density of 1,0 x 10(6)cells/cm(2) on the other side of the sponge. Finally, we cultured this for 1 wk and implanted it on the backs of severe combined immunodeficiency mice with or without basic FGF. RESULTS: Six weeks after implantation, specimens were harvested. Macroscopically, the formed tissue in the bFGF-administered group was thick, and the epidermal component, the dermal component, and adipose tissue were formed in the cross section. The thickness of newly formed tissue in bFGF-administered group was significantly greater than that in the group without bFGF administration. The area of the newly formed capillaries in the bFGF-administered group was significantly larger than that in the group without bFGF administration. CONCLUSIONS: We could produce a thick composite tissue in vivo, combining three kinds of human cells, collagen scaffold, and bFGF. This composite graft was thicker than the bilayered dermal substitute and could be a substitute for a skin flap.

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The aim of this study was to evaluate the effects of combining a porous poly(L-lactide-co-epsilon-caprolactone)/beta-tricalcium phosphate membrane and gelatin sponge incorporating basic fibroblastic growth factor (bFGF) on bone regeneration in mandibular ridges. Four full-thickness saddle-type defects (10 mm long x 5 mm deep) were symmetrically created in both edentulous mandibular alveolar ridges of 6 beagles. The dome-shaped membrane was secured to each defect site, and a gelatin sponge containing 200 microg bFGF was implanted on the left side of each defect (experimental group). Only the membranes (control group) were secured to the defect sites on the right. Three and 6 months later, 3 animals were killed. Bone regeneration was analyzed by soft X-ray photographs, micro-computed tomography (CT) images, and peripheral quantitative CT (pQCT), and then examined histologically. Soft X-ray examination revealed an increase in new bone volume in the experimental group 6 months postoperatively. pQCT showed that immature bone density was higher in the experimental group. Micro-CT images revealed well formed new bone along the original contour of the dome-shaped membrane in the experimental group. Histologically, inflammatory infiltration of tissue surrounding the membranes was slight. These results suggest that combining the poly(L-lactide-co-epsilon-caprolactone)/beta-tricalcium phosphate membrane and bFGF-gelatin sponge is promising for alveolar ridge reconstruction.

DOI： 10.1016/j.ijom.2007.11.010

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Informa UK Limited  

Cultured skin substitutes (CSS) with both epidermal and dermal components seem to be ideal, but they have not been widely used clinically, partly because it takes several weeks to produce them. Decreasing the number of seeding cells may reduce the period required for production, but it still takes a long time before the cells become confluent and neovascularisation is completed in CSS after grafting. As we have already succeeded in reducing the number of seeded keratinocytes in this study, we first attempted to reduce the number of seeded fibroblasts. Consequently, preconfluent CSS with 10 x 10(3) cells/cm2 of fibroblasts combined with 100 x 10(3) cells/cm2 of keratinocytes could be successfully grafted on to full-thickness wounds. bFGF-impregnated gelatin microspheres were then added to the preconfluent CSS before grafting. Incorporation of bFGF significantly accelerated neovascularisation and increased epidermal thickness, cellular components, and thickness of the dermis. The incorporation of bFGF makes CSS a potential therapeutic approach for management of skin wounds.

DOI： 10.1080/02844310701384041

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記述言語：日本語   出版者・発行元：日本再生歯科医学会  

我々は,二種類のコラーゲンスポンジと二種類のヒト皮膚由来の正常細胞(真皮繊維芽細胞&表皮角化細胞)からなる新しい三次元培養皮膚モデル"Vitrolife-SkinTM"を開発した.この培養皮膚モデルは,真皮層と角質層を含む表皮層の二層構造を有しており,実際の皮膚に類似した構造となっている.この培養皮膚モデルを用いて,皮膚刺激性や皮膚腐食性試験などの動物実験代替法の検討を行い,新たな被験物質作用方法の開発を行っている.また,この培養皮膚モデルを用いた皮膚組織再生のための臨床応用に向けた検討も行っている.本稿では,我々の培養皮膚モデルについて概説する(著者抄録)

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その他リンク： https://search.jamas.or.jp/default/link?pub_year=2005&ichushi_jid=J04104&link_issn=&doc_id=20060210130002&doc_link_id=%2Fdi3regde%2F2005%2F000301%2F002%2F0012-0022%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdi3regde%2F2005%2F000301%2F002%2F0012-0022%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

記述言語：日本語   出版者・発行元：(株)医学書院  

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その他リンク： https://search.jamas.or.jp/default/link?pub_year=2005&ichushi_jid=J01537&link_issn=&doc_id=20051109440046&doc_link_id=10.11477%2Fmf.1410100230&url=https%3A%2F%2Fdoi.org%2F10.11477%2Fmf.1410100230&type=%E5%8C%BB%E6%9B%B8.jp_%E3%82%AA%E3%83%BC%E3%83%AB%E3%82%A2%E3%82%AF%E3%82%BB%E3%82%B9&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND: Fibroblast-seeded collagen sponges have been used for the treatment of skin defects and skin ulcers. However, the viability of the fibroblasts after implantation is still unknown. The objective of this study was to investigate the viability and distribution of autologous and allogeneic fibroblasts after implantation and to clarify which type is more effective for wound healing. MATERIALS AND METHODS: Skin samples of Hartley guinea pigs were retrieved and autologous fibroblasts were isolated and cultured. Fibroblasts isolated from the skin of a Strain2 guinea pig were used as allogeneic fibroblasts. Three full-thickness wounds were created on the backs of guinea pigs and an acellular collagen sponge, a collagen sponge seeded with autologous fibroblasts, and a collagen sponge seeded with allogeneic fibroblasts were transplanted. Before implantation, fibroblasts were labeled with PKH26. The guinea pigs were sacrificed 1, 2, and 3 weeks after implantation. The epithelization and contraction of the wounds were assessed, and the viability and distribution of the seeded fibroblasts were observed in cross sections. RESULTS: Three weeks after implantation, the PKH26-labeled autologous and allogeneic fibroblasts remained viable. In the wounds covered with the autologous fibroblast-seeded collagen sponge, the epithelization was fastest, and the percent wound contraction was smallest. In contrast, in the wounds covered with allogeneic fibroblasts, the epithelization was slowest and the percent contraction was largest. CONCLUSION: The allogeneic fibroblasts seeded in the collagen sponge survived and remained viable on the grafted area, but did not accelerate wound healing.

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

A monofilament suture made of poly(L-lactide-co-epsilon-caprolactone) was examined by several mechanical tests to evaluate handling characteristics for tight tying. Six types of other monofilament sutures were also examined for comparisons. Two of these were nonabsorbable, while the others were absorbable sutures. Sutures consisting of glicolide were strongest among all the sutures examined. On the other hand, PROLENE and P(LA/CL) sutures showed high knot-pull strength despite low straight pull strength. Untying performance was evaluated by viscoelasticity, bending plasticity and tying test. A good correlation between tan delta and bending plasticity index was observed and the poly(L-lactide-co-epsilon-caprolactone) suture exhibited high tan delta, high bending plasticity and good resistance against untying.

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記述言語：日本語   出版者・発行元：(一社)日本熱傷学会  

培養人工皮膚作製時におけるウシ胎仔血清(FCS)の添加が培養皮膚接着に及ぼす影響について検討した.その結果,in vitroでの培養人工皮膚における細胞増殖においては播種した線維芽細胞,表皮角化細胞とも,FCS添加により細胞増殖は有意に促進された.しかし,FCS添加の有無にかかわらず,免疫抑制動物に移植した培養人工皮膚は生着し,表皮は重層化され,基底層・有棘層・顆粒層・角層の形成を認めた

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記述言語：日本語   出版者・発行元：(一社)日本熱傷学会  

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記述言語：日本語   出版者・発行元：(一社)日本熱傷学会  

bFGF含浸ゼラチンマイクロスフィアーを培養皮膚に組み込み,培養皮膚の上皮化促進効果を検討した.移植4日目のbFGF徐放群では対照群と比較してコラーゲンスポンジの下層に線維芽細胞の侵入が明らかで,又,多くの赤血球も確認でき,血管の新生が始まっていると考えられた.移植7日目には,bFGF徐放群は真皮層のかなり上層まで多くの線維芽細胞,赤血球が確認できた.移植2週間後ではbFGF徐放群,対象群とも表皮層は重層化し,コラーゲンスポンジ内も上層まで線維芽細胞侵入がみられるが,bFGF徐放群の方が表皮の重層化は厚く,角層も観察できた.bFGF徐放群では表皮層下縁に沿って染色され,基底膜の再構築が進んた.ゼラチンマイクロスフィアーを用いbFGFを徐放させることで,cell-preconfluentな培養皮膚の移植後上皮化完了促進効果が認められた

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記述言語：日本語   出版者・発行元：(一社)日本熱傷学会  

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記述言語：日本語   出版者・発行元：日本バイオマテリアル学会  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley  

Recently a new resorbable P(LA/CL) monofilament suture made of L-lactide and epsilon-caprolactone was developed. The sensitization test was carried out using a guinea pig maximization test according to the Japanese Guidelines. As a result of this test, it was revealed that the methanol extract of P(LA/CL) suture contains a skin sensitizer. It was suggested that the D&C Violet No. 2 contained in P(LA/CL) sutures is decomposed during the melt-spinning process and that the decomposed dye induces skin sensitization. To characterize the sensitizer (the decomposed dye) it was separated and collected by HPLC. To determine the molecular formula of the sensitizer, MS, IR, and NMR spectra were obtained. The molecular weight of the sensitizer was estimated as 240 by measuring the MS. Considering the chemical structure of the dye and the molecular weights, it was determined that the sensitizer was 1,4-dihydroxyanthraquinone (quinizarin). The D&C Violet No. 2 contained in P(LA/CL) sutures was decomposed by the melt-spinning procedure to form quinizarin, one type of anthraquinone dye, and the sensitizer was identified to be, indeed, quinizarin. To evaluate the sensitizing potential of quinizarin, we tested skin sensitization using the maximization test method. Quinizarin induced skin sensitization in guinea pigs, and the minimum sensitizing concentration was approximately 100 ppm. The concentration of quinizarin contained in the P(LA/CL) suture was 1-5 ppm, a value that is lower than the minimum sensitizing concentration of quinizarin. This suggests that P(LA/CL) suture has a low risk with regard to contact dermatitis.

DOI： 10.1002/1097-4636(20010315)54:4<531::aid-jbm90>3.0.co;2-#

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その他リンク： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/1097-4636%2820010315%2954%3A4%3C531%3A%3AAID-JBM90%3E3.0.CO%3B2-%23

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley  

Hydrolytic degradation of two nonabsorbable sutures, four absorbable sutures, and a new type of absorbable suture was studied in buffered media of various pHs at 37 degrees C. The pH levels fixed in this study were 1.0, 7.4, 8.5, and 10.5. Physical measurements were made on the retention of tensile strength and melting temperature of the sutures after hydrolysis for 12 weeks. Sutures containing glycolic acid as a comonomer exhibited enhanced degradation in alkaline media, similar to polyglycolide multifilament sutures. Poly-p-dioxanone (PDS II) suture lost strength to a significant extent at pH 1.0, suggesting that care should be taken when this suture is used for closing tissues in contact with acidic media, such as the stomach. In marked contrast, the degradation of lactide-epsilon-caprolactone copolymer [P(LA/CL)] suture was not sensitive to the pH of media. The surface morphology of hydrolyzed sutures varied, depending on the pH of media. Particularly, moon-crater-shaped impressions were observed on glycolide-epsilon-caprolactone copolymer (MONOCRYL) and glycolide-trimethylene carbonate-dioxanone copolymer (BIOSYN) sutures. Among the nonabsorbable sutures, nylon (ETHILON) exhibited the fastest loss of strength in acidic buffer solution, and polypropylene (PROLENE) suture retained most of its initial strength at all pHs studied.

DOI： 10.1002/jbm.1048

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Informa UK Limited  

Human amniotic membrane was cross-linked with chemical and radiation methods to investigate the effect of cross-linking on its physicochemical and biodegradation properties. Radiation cross-linking was performed with gamma-ray and electron beam while chemical cross-linking was with glutaraldehyde (GA). Both gamma-ray and electron beam irradiation decreased the tensile strength and elongation at break of the amniotic membrane with an increase in the irradiation dose, whereas GA cross-linking had no effect on the tensile properties. This is probably due to the scission of collagen chains through irradiation. No significant change was observed on the water content of cross-linked amniotic membranes for any of the crosslinking methods and in marked contrast with cross-linking of a gelatin membrane. A permeation study revealed that protein permeation through the amniotic membrane was not influenced by the GA concentration at cross-linking. These findings are ascribed to the structure characteristic of the amniotic membrane. The membrane is composed of a fibrous mesh structure from an assemblage of collagen fibers. It is possible that cross-linking takes place in the interior of the fiber assembly without impairing the mesh structure, resulting in no change of the water content and protein permeability. In vitro degradation of cross-linked amniotic membranes revealed that radiation cross-linking appeared to be much less effective than GA cross-linking in retarding the degradation, probably because of low cross-linking densities. GA-cross-linked amniotic membranes were degraded more slowly as the GA concentration at cross-linking increased. When the GA-cross-linked amniotic membrane was subcutaneously implanted in the rat, the tissue response was mild, similar to that of the non-cross-linked native membrane.

DOI： 10.1163/156856299x00829

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掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

DOI： 10.1016/s0141-3910(97)00183-3

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掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley  

DOI： 10.1002/(sici)1099-0518(19971130)35:16<3553::aid-pola22>3.0.co;2-d

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Hyaluronic acid (HA) was chemically crosslinked with a water-soluble carbodiimide (WSC) to produce low-water-content films when brought into contact with water. The crosslinking reaction was performed in two different ways; one was by using HA films and the other by casting HA solutions. Both methods produced water-insoluble HA films. The lowest water content of the crosslinked HA films subjected to swelling with water was 60 wt % at 37 degrees C, which was lower than any reported values. Infrared spectra of the crosslinked films suggested that intermolecular formation of ester bonds between the hydroxyl and carboxyl groups belonging to different polysaccharide molecules led to crosslinking. For comparison, pectin which possesses hydroxyl and carboxyl groups in one molecule, similar to HA, was subjected to crosslinking with WSC. The finding on pectin also supported ester formation between different polysaccharide molecules. The crosslinking of HA film with WSC in the presence of L-lysine methyl ester prolonged the in vivo degradation of HA film, probably because of amide bond formation as the crosslink.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

Chitin was deacetylated to various extents with NaOH to obtain partially and thoroughly deacetylated chitins. The specimens used in this study were deacetylated by 0 (chitin), 68.8, 73.3, 84.0, 90.1 and 100 mol% (chitosan). Films with a thickness of 150 microns were prepared from these specimens by the solution casting method. The equilibrated water contents of the films were 52.4 (chitin), 73.8 (68.8 mol%), 64.2 (73.3 mol%), 61.8 (84.0 mol%), 57.8 (90.1 mol%) and 49.7 wt% (chitosan), while the tensile strengths of the water-swollen films were 244 (chitin), 197 (68.8 mol%), 232 (73.3 mol%), 320 (84.0 mol%), 293 (90.1 mol%) and 433 g mm-2 (chitosan). The maximum water content and the minimum tensile strength observed for a specimen deacetylated between 0 and 68.8 mol% may be ascribed to the lowered crystallinity by deacetylation of chitin, since both chitin and chitosan are crystalline polymers. Unlike their physical properties, in vitro and in vivo degradations of these films occurred less rapidly without passing a maximum or minimum, as their degree of deacetylation became higher. The in vitro degradation was carried out by immersing the films in buffered aqueous solution of pH 7 containing lysozyme at 37 degrees C, while the in vivo degradation was studied by subcutaneously implanting the films in the back of rats. It was found that the rate of in vivo biodegradation was very high for chitin and 68.8 mol% deacetylated chitin, compared with that for the 73.3 mol% deacetylated chitin. The films which were more than 73.3 mol% deacetylated showed slower biodegradation. Interestingly, the tissue reaction towards highly deacetylated derivatives including chitosan was very mild, although they had cationic primary amines in the molecule.

DOI： 10.1016/s0142-9612(96)00167-6

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

Hyaluronic acid (HA) was chemically cross-linked with poly(ethylene glycol) diglycidyl ether, a diepoxy compound (EX-810), to yield low water content and slowly degradable films when brought into contact with water. The cross-linking reaction was performed under acidic and neutral conditions, since the epoxy group is readily hydrolysed in alkaline media. To allow the reaction to proceed at high HA concentrations, a solution casting method was employed for the cross-linking of HA. The lowest water content of the cross-linked HA films obtained was 60 wt% when swollen with buffered saline at 37 degrees C. Alginic acid and poly(vinyl alcohol), which possess hydroxyl groups, similar to HA, were also found to undergo cross-linking with the diepoxy compound. Since IR spectra of the cross-linked films had no significantly new absorption, intermolecular formation of ether bonds between the hydroxyl groups belonging to different polysaccharide molecules was assumed to take place. It seemed too difficult to detect the ether bonds in the cross-linked HA films, because the virgin HA film itself contained ether bonds in the molecule. The cross-linked HA film with a water content of 60 wt% exhibited practically no weight loss after 10 days of immersion in phosphate-buffered saline (pH 7.4), while this film underwent in vivo degradation by 30% weight loss after 7 days of subcutaneous implantation in rats. The inflammation reaction elicited around the implanted film was not significant.

DOI： 10.1016/s0142-9612(96)00116-0

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Carbodiimides were employed for cross-linking of gelatin through amide bond formation to avoid any foreign bond incorporation into the cross-linked gelatin molecules. Cross-linking of gelatin was performed not in aqueous solution but in the form of a film under a heterogenous condition. Ethanol-water mixtures were used as the reaction medium to prevent dissolution of gelatin films. The optimal ethanol concentration in the ethanol-water mixtures was around 80 vol%, and the water content of the cross-linked gelatin film attained after swelling with water at 25 degrees C was as low as 55 wt%. A water-soluble carbodiimide (l-ethyl-3[3-dimethylaminoprophyl] carbodiimide) was more effective for gelatin cross-linking than a water-insoluble carbodiimide (1,3-dicyclohexyl carbodiimide). The optimal temperature for cross-linking with the water-soluble carbodiimide ranged between 15 and 25 degrees C. It was concluded that gelatin cross-linking with carbodiimides is as effective as that with glutaraldehyde, which is most widely used despite the toxicity, at least, so far as the water content of cross-linked gelatin film is concerned.

DOI： 10.1089/ten.1996.2.307

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

A new tracheal prosthesis was made from fine Marlex mesh reinforced with a continuous polypropylene spiral. The mesh and spiral were covalently grafted and further coated with pig collagen with the aim of promoting connective tissue infiltration and providing initial airtightness. Complete surgical resection and replacement of a segment (2 cm in length, three to five tracheal rings) of the cervical trachea was performed in 13 adult mongrel dogs. Two dogs died of pneumonia about 2 months after operation, and eleven dogs were killed between 3 and 26 months. The prostheses in all dogs were promptly infiltrated by the surrounding connective tissue and completely incorporated by the host trachea. Formation of respiratory epithelium, which lined the prosthetic lumen, was seen to various degrees, and, in five dogs killed at 6 months or more after reconstruction, confluent epithelialization was confirmed histologically from the upper to the lower anastomotic site of the prosthesis. Marked stenosis of the prosthetic lumen caused by excessive scar tissue growth was seen in three dogs, and ulceration on the luminal surface was seen in two dogs. These results indicate that this tracheal prosthesis is highly biocompatible and promising for the repair of tracheal defects after further investigation.

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出版者・発行元：JAPANESE SOCIETY FOR ARTIFICIAL ORGANS  

雑種成犬を用い我々が独自に作製したメッシュ製人工気管で縦隔気管の管状置換実験を行った。人工気管はMarlex® meshとpolypropylene製の支持体よりなり、ブタ・コラーゲンを複合化したものである。雑種成犬6頭を全身麻酔下に右開胸し、縦隔気管の管状切除(1頭は約2cm、4気管輪、5頭は約4cm、7-8気管輪)を行い、それぞれ全長3cmと5cmの人工気管で置換した。5cm人工気管を使用した5頭では、術後材料内腔への喀痰の付着防止の目的で1ヵ月間シリコン製チューブを内挿留置した。施行した6頭は全て術後、吻合部の離開、気漏は見られなかった。4ヵ月より26カ月の観察期間において1頭で内腔の軽度狭窄を認め、5頭で材料の部分的な露出(潰瘍化)を認めた。材料内腔の上皮化は種々の程度で見られ、2cmの置換を行った1頭では頭側端より尾側端まで連続する気管上皮の再生が組織学的に確認された。今後の臨床応用の可能性が示唆された。

DOI： 10.11392/jsao1972.23.878

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記述言語：英語   出版者・発行元：JAPANESE SOCIETY FOR ARTIFICIAL ORGANS  

メッシュ式人工気管による高張力下での置換実験を行った。この人工気管はMarlex® meshとpolypropylene stentによる支持体からなり、これにコラーゲンを複合化したものである。雑種成犬を用い、吻合時の両端の張力が900-1000g重になるように頸部気管を管状切除(6-8cm、11-16気管軟骨輪)、人工気管で置換した。この際4頭は置換のみ(A群)、6頭はさらにシリコンチューブを術後1カ月間置換部に内挿した(B群)。全例縫合不全は認めなかった。A群では局所感染、過剰肉芽による内腔狭窄が目だったが、B群では経過良好であり、術後3カ月で屠殺した犬では、人工気管は宿主気管と完全に一体化し、内腔の上皮化も見られた。以上より我々の人工気管は張力のかかった条件下でも置換に耐え、臨床応用が可能性であることが示唆された。またシリコンチューブの一時的内挿は材料の感染を予防するのに有用と考えられた。

DOI： 10.11392/jsao1972.22.1025

CiNii Article

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その他リンク： http://search.jamas.or.jp/link/ui/1994031485

記述言語：日本語   出版者・発行元：JAPANESE SOCIETY FOR ARTIFICIAL ORGANS  

我々は、長さ10mmのラットの坐骨神経の欠損を熱脱水架橋処理ゼラチンチューブ及びグルタールデヒド処理ゼラチンチューブを用いて連結し、坐骨神経の再生回復の状態を観察した。熱架橋処理ゼラチンチューブ群では1ヶ月後にはチューブの壁内に血管網が形成され、2ヶ月後にはチューブは分解吸収されており、坐骨神経が再生していた。手術後2ヶ月及び4ヶ月において行ったOsmium染色、Bodian染色及びS-100蛋白染色による形態的観察では、2ヶ月から再生軸索が数多く認められ遠位端に達しており、髄鞘も再生していた。WGA-HRP染色では、脊髄前角の運動神経細胞と後角の知覚神経終末が染色された。機能的にも電気刺激による誘発筋電図、大脳体性感覚誘発電位が回復していた。一方、グルタルアルデハイド処理ゼラチンチューブ群では神経の再生は認められなかった。これらの結果から、熱脱水架橋ゼラチンチューブは神経再生チャンネルとして優れた医用材料になり得ると結論された。

DOI： 10.11392/jsao1972.22.364

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The authors studied the efficacy of a new tracheal prosthesis made from mesh. The prosthesis, 50 mm long and 18-22 mm in diameter, is made from a Marlex mesh cylinder reinforced with a continuous polypropylene spiral that is grafted and coated with porcine collagen to increase its biocompatibility and provide an airtight seal during the initial implantation stage. Circumferential surgical resection and replacement of a seven to nine ring segment of the cervical trachea was performed in 20 adult mongrel dogs. At the time of surgery, a silicone tube was inserted into the tracheal prosthetic lumen to promote secretory transportation until the prosthesis was covered with host tissue. The silicone tube was removed during fiber bronchoscopy 1 month after surgery. With the exception of the prostheses in 3 dogs that died of unrelated causes, all were infiltrated by connective tissue and incorporated completely by the host. One of these 17 dogs died of suffocation caused by luminal stenosis 2.5 months after surgery, but the others survived until they were killed at more than 6 months. The luminal surfaces of the reconstructed tracheae were covered with respiratory epithelium to varying degrees, and in one dog killed at 22 months after surgery, confluent epithelization throughout the length of the prosthesis was confirmed histologically. In eight dogs, prosthetic luminal stenosis occurred because of overgrowth of granulation tissue, which generally was mild in all but three dogs. The authors conclude that this tracheal prosthesis is highly biocompatible and shows promise for clinical application.

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

This study compares the use of synthetic bioabsorbable mesh with that of non-absorbable mesh in repairing tracheal defects. Two patch windows, each 1 cm x 1 cm, were created on the ventral wall of the cervical trachea in 12 adult mongrel dogs. One window was repaired using a bioabsorbable polyglycolic acid (PGA) mesh graft, and the other using a nonabsorbable Marlex mesh (polypropylene mesh; PP mesh) (C. R. Bard, Inc., Billerica, MA) graft. The dogs were killed at intervals from 1 to 35 weeks after surgery. Macroscopically, there was no difference between the two mesh groups until 3 weeks after surgery. At 6 weeks in the PP mesh group, the connective tissue over the mesh was remarkably thick, and after 9 weeks, constriction by scarlike tissue was evident, as compared with the PGA mesh group, which showed no constriction. Microscopically, epithelium covered the whole mesh in both groups at 6 weeks. However, in the PP mesh group, flat and poorly differentiated cells occupied a large area, and after 9 weeks the height of the epithelial cells in the PP mesh group was less than in the PGA mesh group. Furthermore, in the PP mesh group, nonciliated cells occupied a large area even at 15 weeks, and the submucous connective tissue layer was thicker than in the PGA mesh group after 6 weeks. The results indicate that, from the point of view of wound-healing, bioabsorbable mesh may be more adequate for repairing tracheal defects.

PubMed

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記述言語：日本語   出版者・発行元：(一社)日本創傷治癒学会  

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記述言語：日本語   出版者・発行元：(一社)日本熱傷学会  

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記述言語：日本語   出版者・発行元：(一社)日本創傷治癒学会  

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記述言語：日本語   出版者・発行元：(一社)日本褥瘡学会  

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記述言語：日本語   出版者・発行元：日本バイオマテリアル学会  

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出願人：グンゼ株式会社

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出願人：グンゼ株式会社

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公開番号：特開2007-228873  公開日：2007年9月

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出願人：グンゼ株式会社

出願番号：特願2005-261840  出願日：2005年9月

公開番号：特開2007-068884  公開日：2007年3月

特許番号/登録番号：特許第5008284号  登録日：2012年6月 

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出願人：グンゼ株式会社

出願番号：特願2005-261840  出願日：2005年9月

公開番号：特開2007-068884  公開日：2007年3月

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出願人：グンゼ株式会社

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出願人：グンゼ株式会社

出願番号：特願2004-024263  出願日：2004年1月

公開番号：特開2005-213449  公開日：2005年8月

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出願人：グンゼ株式会社

出願番号：特願2003-056505  出願日：2003年3月

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特許番号/登録番号：特許第4293518号  登録日：2009年4月 

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出願人：グンゼ株式会社

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公開番号：特開2004-261133  公開日：2004年9月

J-GLOBAL

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出願人：グンゼ株式会社

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出願人：グンゼ株式会社

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公開番号：特開2004-217290  公開日：2004年8月

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出願人：グンゼ株式会社

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特許番号/登録番号：特許第4373658号  登録日：2009年9月 

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出願人：グンゼ株式会社

出願番号：特願2002-289573  出願日：2002年10月

公開番号：特開2004-121523  公開日：2004年4月

J-GLOBAL

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出願人：グンゼ株式会社

出願番号：特願2002-141348  出願日：2002年5月

公開番号：特開2003-325652  公開日：2003年11月

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出願人：グンゼ株式会社

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出願人：グンゼ株式会社

出願番号：特願平11-125420  出願日：1999年5月

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出願人：グンゼ株式会社

出願番号：特願平11-125420  出願日：1999年5月

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出願人：グンゼ株式会社

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公開番号：特開2000-102599  公開日：2000年4月

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出願人：グンゼ株式会社

出願番号：特願平10-276751  出願日：1998年9月

公開番号：特開2000-102599  公開日：2000年4月

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出願人：グンゼ株式会社

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出願人：グンゼ株式会社

出願番号：特願平7-166900  出願日：1995年6月

公開番号：特開平8-333402  公開日：1996年12月

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出願人：グンゼ株式会社

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出願人：グンゼ株式会社

出願番号：特願平7-152596  出願日：1995年5月

公開番号：特開平8-317968  公開日：1996年12月

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出願人：グンゼ株式会社

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公開番号：特開平7-102002  公開日：1995年4月

特許番号/登録番号：特許第3404557号  登録日：2003年3月 

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出願人：グンゼ株式会社

出願番号：特願平5-245072  出願日：1993年9月

公開番号：特開平7-102002  公開日：1995年4月

J-GLOBAL

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