2024/06/18 更新

写真a

シヤバン アミナ カボソ
SHABAN AMINA KABOSO
SHABAN Amina Kaboso
所属
教育研究院 医歯学系 医学系列 助教
医学部 医学科 助教
医歯学総合研究科 地域疾病制御医学専攻 国際感染医学 助教
職名
助教
連絡先
メールアドレス
外部リンク

学位

  • Msc. Biomedical science ( 2019年9月   Niigata University )

  • Bsc. Biomedical science and technology ( 2011年12月 )

研究キーワード

  • Infectious disease, Bacteria, Tuberculosis, Immunology, Vaccines

研究分野

  • ライフサイエンス / 細菌学  / Tuberculosis

経歴(researchmap)

  • 新潟大学   Bacteriology   Assistant Professor

    2024年4月 - 現在

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  • 新潟大学   Bacteriology   Specially Appointed Assistant

    2023年8月 - 2024年3月

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経歴

  • 新潟大学   教育研究院 医歯学系 医学系列   助教

    2024年4月 - 現在

  • 新潟大学   医歯学総合研究科 地域疾病制御医学専攻 国際感染医学   助教

    2024年4月 - 現在

  • 新潟大学   医学部 医学科   助教

    2024年4月 - 現在

  • 新潟大学   医歯学総合研究科   特任助手

    2023年8月 - 2024年3月

  • 新潟大学   教育研究院 医歯学系   特任助手

    2023年8月 - 2024年3月

学歴

  • 新潟大学   大学院医歯学総合研究科   Msc.

    2017年10月 - 2019年9月

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    備考: Msc. Biomedical science

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  • 新潟大学   大学院医歯学総合研究科   PhD.

    2019年10月

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所属学協会

  • The Molecular Biology Society of Japan

    2020年 - 2023年

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  • 日本細菌学会中部支部会

    2018年8月 - 現在

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論文

  • Recombinant mycobacterial DNA-binding protein 1 with post-translational modifications boosts IFN-gamma production from BCG-vaccinated individuals' blood cells in combination with CpG-DNA. 国際誌

    Yuriko Ozeki, Akira Yokoyama, Akihito Nishiyama, Yutaka Yoshida, Yukiko Ohara, Tsukasa Mashima, Chikako Tomiyama, Amina K Shaban, Atsuki Takeishi, Mayuko Osada-Oka, Takehiro Yamaguchi, Yoshitaka Tateishi, Jun-Ichi Maeyama, Mariko Hakamata, Hiroshi Moro, Toshiaki Kikuchi, Daisuke Hayashi, Fumiko Suzuki, Toshiko Yamamoto, Sumiko Iho, Masato Katahira, Saburo Yamamoto, Sohkichi Matsumoto

    Scientific reports   14 ( 1 )   9141 - 9141   2024年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Tuberculosis remains a large health threat, despite the availability of the tuberculosis vaccine, BCG. As BCG efficacy gradually decreases from adolescence, BCG-Prime and antigen-booster may be an efficient strategy to confer vaccine efficacy. Mycobacterial DNA-binding protein 1 (MDP1, namely Rv2986c, hupB or HU) is a major Mycobacterium tuberculosis protein that induces vaccine-efficacy by co-administration with CpG DNA. To produce MDP1 for booster-vaccine use, we have created recombinant MDP1 produced in both Escherichia coli (eMDP1) and Mycolicibacterium smegmatis (mMDP1), an avirulent rapid-growing mycobacteria. We tested their immunogenicity by checking interferon (IFN)-gamma production by stimulated peripheral blood cells derived from BCG-vaccinated individuals. Similar to native M. tuberculosis MDP1, we observed that most lysin resides in the C-terminal half of mMDP1 are highly methylated. In contrast, eMDP1 had less post-translational modifications and IFN-gamma stimulation. mMDP1 stimulated the highest amount of IFN-gamma production among the examined native M. tuberculosis proteins including immunodominant MPT32 and Antigen 85 complex. MDP1-mediated IFN-gamma production was more strongly enhanced when combined with a new type of CpG DNA G9.1 than any other tested CpG DNAs. Taken together, these results suggest that the combination of mMDP1 and G9.1 possess high potential use for human booster vaccine against tuberculosis.

    DOI: 10.1038/s41598-024-58836-8

    PubMed

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  • Genetic engineering employing MPB70 and its promoter enables efficient secretion and expression of foreign antigen in bacillus Calmette Guérin (BCG) Tokyo

    Atsuki Takeishi, Amina K. Shaban, Taichi Kakihana, Hayato Takihara, Shujiro Okuda, Hidekazu Osada, Desak Nyoman Surya Suameitria Dewi, Yuriko Ozeki, Yutaka Yoshida, Akihito Nishiyama, Yoshitaka Tateishi, Yuki Aizu, Yasushi Chuma, Kazuyo Onishi, Daisuke Hayashi, Saburo Yamamoto, Tetsu Mukai, Manabu Ato, Duong Huu Thai, Huynh Thi Thao Nhi, Tsuyoshi Shirai, Satoshi Shibata, Fumiko Obata, Jun Fujii, Seiya Yamayoshi, Maki Kiso, Sohkichi Matsumoto

    Microbiology and Immunology   2024年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Abstract

    Vaccination is an important factor in public health. The recombinant bacillus Calmette Guérin (rBCG) vaccine, which expresses foreign antigens, is expected to be a superior vaccine against infectious diseases. Here, we report a new recombination platform in which the BCG Tokyo strain is transformed with nucleotide sequences encoding foreign protein fused with the MPB70 immunogenic protein precursor. By RNA‐sequencing, mpb70 was found to be the most transcribed among all known genes of BCG Tokyo. Small oligopeptide, namely, polyhistidine tag, was able to be expressed in and secreted from rBCG through a process in which polyhistidine tag fused with intact MPB70 were transcribed by an mpb70 promoter. This methodology was applied to develop an rBCG expressing the receptor binding domain (RBD) of severe acute respiratory syndrome coronavirus 2. Immunoblotting images and mass spectrometry data showed that RBD was also secreted from rBCG. Sera from mice vaccinated with the rBCG showed a tendency of weak neutralizing capacity. The secretion was retained even after a freeze‐drying process. The freeze‐dried rBCG was administered to and recovered from mice. Recovered rBCG kept secreting RBD. Collectively, our recombination platform offers stable secretion of foreign antigens and can be applied to the development of practical rBCGs.

    DOI: 10.1111/1348-0421.13116

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  • Dynamic action of an intrinsically disordered protein in DNA compaction that induces mycobacterial dormancy

    Akihito Nishiyama, Masahiro Shimizu, Tomoyuki Narita, Noriyuki Kodera, Yuriko Ozeki, Akira Yokoyama, Kouta Mayanagi, Takehiro Yamaguchi, Mariko Hakamata, Amina Kaboso Shaban, Yoshitaka Tateishi, Kosuke Ito, Sohkichi Matsumoto

    Nucleic Acids Research   2023年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    Abstract

    Mycobacteria are the major human pathogens with the capacity to become dormant persisters. Mycobacterial DNA-binding protein 1 (MDP1), an abundant histone-like protein in dormant mycobacteria, induces dormancy phenotypes, e.g. chromosome compaction and growth suppression. For these functions, the polycationic intrinsically disordered region (IDR) is essential. However, the disordered property of IDR stands in the way of clarifying the molecular mechanism. Here we clarified the molecular and structural mechanism of DNA compaction by MDP1. Using high-speed atomic force microscopy, we observed that monomeric MDP1 bundles two adjacent DNA duplexes side-by-side via IDR. Combined with coarse-grained molecular dynamics simulation, we revealed the novel dynamic DNA cross-linking model of MDP1 in which a stretched IDR cross-links two DNA duplexes like double-sided tape. IDR is able to hijack HU function, resulting in the induction of strong mycobacterial growth arrest. This IDR-mediated reversible DNA cross-linking is a reasonable model for MDP1 suppression of the genomic function in the resuscitable non-replicating dormant mycobacteria.

    DOI: 10.1093/nar/gkad1149

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  • Mycobacterial DNA-binding protein 1 is critical for BCG survival in stressful environments and simultaneously regulates gene expression

    Amina K. Shaban, Gebremichal Gebretsadik, Mariko Hakamata, Hayato Takihara, Erina Inouchi, Akihito Nishiyama, Yuriko Ozeki, Yoshitaka Tateishi, Yukiko Nishiuchi, Takehiro Yamaguchi, Naoya Ohara, Shujiro Okuda, Sohkichi Matsumoto

    Scientific Reports   2023年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <jats:title>Abstract</jats:title><jats:p>Survival of the live attenuated Bacillus Calmette-Guérin (BCG) vaccine amidst harsh host environments is key for BCG effectiveness as it allows continuous immune response induction and protection against tuberculosis. Mycobacterial DNA binding protein 1 (MDP1), a nucleoid associated protein, is essential in BCG. However, there is limited knowledge on the extent of MDP1 gene regulation and how this influences BCG survival. Here, we demonstrate that MDP1 conditional knockdown (cKD) BCG grows slower than vector control in vitro<jats:italic>,</jats:italic> and dies faster upon exposure to antibiotics (bedaquiline) and oxidative stress (H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> and menadione). MDP1-cKD BCG also exhibited low infectivity and survival in THP-1 macrophages and mice indicating possible susceptibility to host mediated stress. Consequently, low in vivo survival resulted in reduced cytokine (IFN-gamma and TNF-alpha) production by splenocytes. Temporal transcriptome profiling showed more upregulated (81–240) than downregulated (5–175) genes in response to MDP1 suppression. Pathway analysis showed suppression of biosynthetic pathways that coincide with low in vitro growth. Notable was the deferential expression of genes involved in stress response (<jats:italic>sigI</jats:italic>), maintenance of DNA integrity (<jats:italic>mutT1</jats:italic>), REDOX balance (<jats:italic>WhiB3</jats:italic>), and host interactions (<jats:italic>PE/PE_PGRS</jats:italic>). Thus, this study shows MDP1’s importance in BCG survival and highlights MDP1-dependent gene regulation suggesting its role in growth and stress adaptation.</jats:p>

    DOI: 10.1038/s41598-023-40941-9

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  • Antibodies against native proteins of Mycobacterium tuberculosis can detect pulmonary tuberculosis patients

    Desak Nyoman Surya Suameitria Dewi, Ni Made Mertaniasih, Soedarsono, Kimika Hagino, Tomoya Yamazaki, Yuriko Ozeki, Wayan Tunas Artama, Haruka Kobayashi, Erina Inouchi, Yutaka Yoshida, Satoshi Ishikawa, Amina Kaboso Shaban, Yoshitaka Tateishi, Akihito Nishiyama, Manabu Ato, Sohkichi Matsumoto

    Scientific Reports   2023年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <jats:title>Abstract</jats:title><jats:p>Accurate point-of-care testing (POCT) is critical for managing tuberculosis (TB). However, current antibody-based diagnosis shows low specificity and sensitivity. To find proper antigen candidates for TB diagnosis by antibodies, we assessed IgGs responsiveness to <jats:italic>Mycobacterium tuberculosis</jats:italic> proteins in pulmonary TB (PTB) patients. We employed major secreted proteins, such as Rv1860, Ag85C, PstS1, Rv2878c, Ag85B, and Rv1926c that were directly purified from <jats:italic>M. tuberculosis</jats:italic>. In the first screening, we found that IgG levels were significantly elevated in PTB patients only against Rv1860, PstS1, and Ag85B among tested antigens. However, recombinant PstS1 and Ag85B from <jats:italic>Escherichia coli (E. coli)</jats:italic> couldn’t distinguish PTB patients and healthy controls (HC). Recombinant Rv1860 was not checked due to its little expression. Then, the 59 confirmed PTB patients from Soetomo General Academic Hospital, Surabaya, Indonesia, and 102 HC were tested to Rv1860 and Ag85B only due to the low yield of the PstS1 from <jats:italic>M. tuberculosis</jats:italic>. The ROC analysis using native Ag85B and Rv1860 showed an acceptable area under curve for diagnosis, which is 0.812 (95% CI 0.734–0.890, <jats:italic>p</jats:italic> &lt; 0.0001) and 0.821 (95% CI 0.752–0.890, <jats:italic>p</jats:italic> &lt; 0.0001). This study indicates that taking consideration of native protein structure is key in developing TB’s POCT by antibody-based diagnosis.</jats:p>

    DOI: 10.1038/s41598-023-39436-4

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  • Lysocin E Targeting Menaquinone in the Membrane of Mycobacterium tuberculosis Is a Promising Lead Compound for Antituberculosis Drugs

    Gebremichal Gebretsadik, Akane Inaizumi, Akihito Nishiyama, Takehiro Yamaguchi, Hiroshi Hamamoto, Suresh Panthee, Aki Tamaru, Manabu Hayatsu, Yusuke Mizutani, Shaban Amina Kaboso, Mariko Hakamata, Aleksandr Ilinov, Yuriko Ozeki, Yoshitaka Tateishi, Kazuhisa Sekimizu, Sohkichi Matsumoto

    Antimicrobial Agents and Chemotherapy   2022年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <jats:p>Tuberculosis remains a public health crisis and a health security threat. There is an urgent need to develop new antituberculosis drugs with novel modes of action to cure drug-resistant tuberculosis and shorten the chemotherapy period by sterilizing tissues infected with dormant bacteria.</jats:p>

    DOI: 10.1128/aac.00171-22

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  • Monitoring IgG against Mycobacterium tuberculosis proteins in an Asian elephant cured of tuberculosis that developed from long-term latency

    Satoshi Ishikawa, Yuriko Ozeki, Satomi Suga, Yasuhiko Mukai, Haruka Kobayashi, Erina Inouchi, Shaban A. Kaboso, Gebremichal Gebretsadik, Desak Nyoman Surya Suameitria Dewi, Akihito Nishiyama, Yoshitaka Tateishi, Hayato Takihara, Shujiro Okuda, Shiomi Yoshida, Naoaki Misawa, Sohkichi Matsumoto

    Scientific Reports   2022年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <jats:title>Abstract</jats:title><jats:p>Tuberculosis (TB) is fatal in elephants, hence protecting elephants from TB is key not only in the conservation of this endangered animal, but also to prevent TB transmission from elephants to humans. Most human TB cases arise from long-term asymptomatic infections. Significant diagnostic challenges remain in the detection of both infection and disease development from latency in elephants due to their huge bodies. In this study, we assessed cryopreserved sera collected for over 16 years, from the first Japanese treatment case of elephant TB. Semi-quantification of IgG levels to 11 proteins showed high detection levels of 3 proteins, namely ESAT6/CFP10, MPB83 and Ag85B. The level of IgG specific to these 3 antigens was measured longitudinally, revealing high and stable ESAT6/CFP10 IgG levels regardless of onset or treatment. Ag85B-specifc IgG levels were largely responsive to onset or treatment, while those of MPB83 showed intermediate responses. These results suggest that ESAT6/CFP10 is immunodominant in both asymptomatic and symptomatic phases, making it useful in the detection of infection. On the other hand, Ag85B has the potential to be a marker for the prediction of disease onset and in the evaluation of treatment effectiveness in elephants.</jats:p>

    DOI: 10.1038/s41598-022-08228-7

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  • Higher genome mutation rates of Beijing lineage of Mycobacterium tuberculosis during human infection

    Mariko Hakamata, Hayato Takihara, Tomotada Iwamoto, Aki Tamaru, Atsushi Hashimoto, Takahiro Tanaka, Shaban A. Kaboso, Gebremichal Gebretsadik, Aleksandr Ilinov, Akira Yokoyama, Yuriko Ozeki, Akihito Nishiyama, Yoshitaka Tateishi, Hiroshi Moro, Toshiaki Kikuchi, Shujiro Okuda, Sohkichi Matsumoto

    Scientific Reports   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <jats:title>Abstract</jats:title><jats:p><jats:italic>Mycobacterium tuberculosis</jats:italic> (<jats:italic>Mtb</jats:italic>) strains of Beijing lineage have caused great concern because of their rapid emergence of drug resistance and worldwide spread. DNA mutation rates that reflect evolutional adaptation to host responses and the appearance of drug resistance have not been elucidated in human-infected Beijing strains. We tracked and obtained an original <jats:italic>Mtb</jats:italic> isolate of Beijing lineage from the 1999 tuberculosis outbreak in Japan, as well as five other isolates that spread in humans, and two isolates from the patient caused recurrence. Three isolates were from patients who developed TB within one year after infection (rapid-progressor, RP), and the other three isolates were from those who developed TB more than one year after infection (slow-progressor, SP). We sequenced genomes of these isolates and analyzed the propensity and rate of genomic mutations. Generation time versus mutation rate curves were significantly higher for RP. The ratio of oxidative versus non-oxidation damages induced mutations was higher in SP than RP, suggesting that persistent <jats:italic>Mtb</jats:italic> are exposed to oxidative stress in the latent state. Our data thus demonstrates that higher mutation rates of <jats:italic>Mtb</jats:italic> Beijing strains during human infection is likely to account for the higher adaptability and an emergence ratio of drug resistance.</jats:p>

    DOI: 10.1038/s41598-020-75028-2

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  • Adduct Formation of Delamanid with NAD in Mycobacteria

    Mikayo Hayashi, Akihito Nishiyama, Ryuki Kitamoto, Yoshitaka Tateishi, Mayuko Osada-Oka, Yukiko Nishiuchi, Shaban A. Kaboso, Xiuhao Chen, Mamoru Fujiwara, Yusuke Inoue, Yoshikazu Kawano, Masanori Kawasaki, Tohru Abe, Tsutomu Sato, Kentaro Kaneko, Kimiko Itoh, Sohkichi Matsumoto, Makoto Matsumoto

    Antimicrobial Agents and Chemotherapy   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <jats:p>
    Delamanid (DLM), a nitro-dihydroimidazooxazole derivative currently approved for pulmonary multidrug-resistant tuberculosis (TB) therapy, is a prodrug activated by mycobacterial 7,8-didemethyl-8-hydroxy 5-deazaflavin electron transfer coenzyme (F
    <jats:sub>420</jats:sub>
    )-dependent nitroreductase (Ddn). Despite inhibiting the biosynthesis of a subclass of mycolic acids, the active DLM metabolite remained unknown. Comparative liquid chromatography-mass spectrometry (LC-MS) analysis of DLM metabolites revealed covalent binding of reduced DLM with a nicotinamide ring of NAD derivatives (oxidized form) in DLM-treated
    <jats:named-content content-type="genus-species">Mycobacterium tuberculosis</jats:named-content>
    var.
    </jats:p>

    DOI: 10.1128/aac.01755-19

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  • 病原性抗酸菌における細胞外DNAの存在と抗酸菌の生理におけるその役割(Existence of extracellular DNA in pathogenic mycobacteria and its role in mycobacterial physiology)

    イリノフ・アレクサンドル, シャバン・アミナ, 袴田 真理子, 西山 晃史, 尾関 百合子, 福島 由華里, 中島 千絵, 立石 善隆, 鈴木 定彦, 松本 壮吉

    日本細菌学雑誌   75 ( 1 )   74 - 74   2020年1月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • C-terminal intrinsically disordered region-dependent organization of the mycobacterial genome by a histone-like protein

    Anna Savitskaya, Akihito Nishiyama, Takehiro Yamaguchi, Yoshitaka Tateishi, Yuriko Ozeki, Masaaki Nameta, Tomohiro Kon, Shaban A. Kaboso, Naoya Ohara, Olga V. Peryanova, Sohkichi Matsumoto

    Scientific Reports   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <jats:title>Abstract</jats:title><jats:p>The architecture of the genome influences the functions of DNA from bacteria to eukaryotes. Intrinsically disordered regions (IDR) of eukaryotic histones have pivotal roles in various processes of gene expression. IDR is rare in bacteria, but interestingly, mycobacteria produce a unique histone-like protein, MDP1 that contains a long C-terminal IDR. Here we analyzed the role of IDR in MDP1 function. By employing <jats:italic>Mycobacterium smegmatis</jats:italic> that inducibly expresses MDP1 or its IDR-deficient mutant, we observed that MDP1 induces IDR-dependent DNA compaction. MDP1-IDR is also responsible for the induction of growth arrest and tolerance to isoniazid, a front line tuberculosis drug that kills growing but not growth-retardated mycobacteria. We demonstrated that MDP1-deficiency and conditional knock out of MDP1 cause spreading of the <jats:italic>M</jats:italic>. <jats:italic>smegmatis</jats:italic> genome in the stationary phase. This study thus demonstrates for the first time a C-terminal region-dependent organization of the genome architecture by MDP1, implying the significance of IDR in the function of bacterial histone-like protein.</jats:p>

    DOI: 10.1038/s41598-018-26463-9

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▶ 全件表示

講演・口頭発表等

  • Mycobacterial DNA binding protein 1 regulates metabolism and replication ensuring the survival of Mycobacterium tuberculosis var BCG

    Amina Kaboso Shaban, Akira Nishiyama, Yoshitaka Tateishi, Yudai Yamaguchi, Yukiko Nishiuchi, Hayato Takihara, Shujiro Okuda, Sokichi Matsumoto

    59th Annual general meeting of the Chubu Branch of Japanese Society of Bacteriology  2022年9月 

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    会議種別:ポスター発表  

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  • MDP1 regulates metabolism and replication ensuring the survival of M. tuberculosis var BCG

    Amina Kaboso Shaban, Akira Nishiyama, Yoshitaka Tateishi, Yudai Yamaguchi, Yukiko Nishiuchi, Hayato Takihara, Shujiro Okuda, Sokichi Matsumoto

    93rd Annual Meeting of Japanese Society for bacteriology  2020年1月 

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    会議種別:ポスター発表  

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  • Mycobacterial DNA binding protein 1 regulates metabolism and replication ensuring the survival of Mycobacterium tuberculosis var. BCG.

    Amina Kaboso Shaban, Akira Nishiyama, Yoshitaka Tateishi, Yudai Yamaguchi, Yukiko Nishiuchi, Hayato Takihara, Shujiro Okuda, Sokichi Matsumoto

    2019年11月 

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    会議種別:口頭発表(一般)  

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受賞

  • Young Medical Research Award

    2020年   Niigata University  

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  • Medical Research Grant

    2019年   Niigata University   Assessment of the role of dormancy in Mycobacterium tuberculosis var. BCG in vitro and in vivo survival using a conditional gene knockdown system

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教育活動に関する受賞

  • Young Medical Research Award

    2020年   Niigata University  

  • Medical Research Grant

    2019年   Niigata University  

 

学術貢献活動

  • 52nd US-Japan Cooperative Medical Sciences Program, Mycobacteria Panel Meeting

    役割:パネル司会・セッションチェア等

    Niigata University, Bacteriology department  2018年3月

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    種別:学会・研究会等 

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