Updated on 2025/06/07

写真a

 
ISHITA Yuki
 
Organization
Brain Research Institute Basic Neuroscience Branch Department of System Pathology for Neurological Disorders Assistant Professor
Title
Assistant Professor
External link

Degree

  • Ph. D. in Science ( 2023.3   Hiroshima University )

Research History

  • Niigata University   Department of System Pathology for Neurological Disorders, Basic Neuroscience Branch, Brain Research Institute   Assistant Professor

    2024.4

 

Papers

  • Co-option of an Astacin Metalloprotease Is Associated with an Evolutionarily Novel Feeding Morphology in a Predatory Nematode

    Yuuki Ishita, Ageha Onodera, Taisuke Ekino, Takahiro Chihara, Misako Okumura

    Molecular Biology and Evolution   40 ( 12 )   2023.12

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    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Animals consume a wide variety of food sources to adapt to different environments. However, the genetic mechanisms underlying the acquisition of evolutionarily novel feeding morphology remain largely unknown. While the nematode Caenorhabditis elegans feeds on bacteria, the satellite species Pristionchus pacificus exhibits predatory feeding behavior toward other nematodes, which is an evolutionarily novel feeding habit. Here, we found that the astacin metalloprotease Ppa-NAS-6 is required for the predatory killing by P. pacificus. Ppa-nas-6 mutants were defective in predation-associated characteristics, specifically the tooth morphogenesis and tooth movement during predation. Comparison of expression patterns and rescue experiments of nas-6 in P. pacificus and C. elegans suggested that alteration of the spatial expression patterns of NAS-6 may be vital for acquiring predation-related traits. Reporter analysis of the Ppa-nas-6 promoter in C. elegans revealed that the alteration in expression patterns was caused by evolutionary changes in cis- and trans-regulatory elements. This study suggests that the co-option of a metalloprotease is involved in an evolutionarily novel feeding morphology.

    DOI： 10.1093/molbev/msad266

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    Other Link： https://academic.oup.com/mbe/article-pdf/40/12/msad266/54912979/msad266.pdf

  • Efficient visual screening of CRISPR/Cas9 genome editing in the nematode Pristionchus pacificus

    Hirokuni Hiraga, Yuuki Ishita, Takahiro Chihara, Misako Okumura

    DEVELOPMENT GROWTH & DIFFERENTIATION   63 ( 9 )   488 - 500   2021.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/dgd.12761

    Web of Science

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  • Different combinations of serotonin receptors regulate predatory and bacterial feeding behaviors in the nematode Pristionchus pacificus

    Yuuki Ishita, Takahiro Chihara, Misako Okumura

    G3-GENES GENOMES GENETICS   11 ( 2 )   2021.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/g3journal/jkab011

    Web of Science

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  • Screening for CRISPR/Cas9-induced mutations using a co-injection marker in the nematode Pristionchus pacificus

    Ken-ichi Nakayama, Yuuki Ishita, Takahiro Chihara, Misako Okumura

    DEVELOPMENT GENES AND EVOLUTION   230 ( 3 )   257 - 264   2020.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00427-020-00651-y

    Web of Science

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  • Serotonergic modulation of feeding behavior in Caenorhabditis elegans and other related nematodes

    Yuuki Ishita, Takahiro Chihara, Misako Okumura

    NEUROSCIENCE RESEARCH   154   9 - 19   2020.5

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Awards

  • 令和４年度広島大学エクセレントスチューデントスカラシップ成績優秀学生

    2022.12   広島大学  

    井下 結葵

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  • 3rd Franco-Japanese Developmental Biology Meeting, Prize for the best oral presentation

    2022.11  

    Yuuki Ishita

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  • 線虫研究の未来を創る会2022 優秀口頭発表賞

    2022.8  

    井下 結葵

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Research Projects

  • アスタシンメタロプロテアーゼから迫る線虫捕食行動のメカニズムとその進化

    Grant number：22KJ2327

    2023.3 - 2024.3

    System name：科学研究費助成事業

    Research category：特別研究員奨励費

    Awarding organization：日本学術振興会

    井下 結葵, 井下 結葵

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    Grant amount：\1700000 （ Direct Cost: \1700000 ）

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  • アスタシンメタロプロテアーゼから迫る線虫捕食行動のメカニズムとその進化

    Grant number：22J13816

    2022.4 - 2024.3

    System name：科学研究費助成事業

    Research category：特別研究員奨励費

    Awarding organization：日本学術振興会

    井下 結葵

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    Grant amount：\1700000 （ Direct Cost: \1700000 ）

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