Faculty Profiles - KOIDE Shingo

Papers

Quantifying subpercent nuclear TDP-43 loss in cells and ALS cortex using junction‐specific cryptic exon RT-qPCR Reviewed

Shingo Koide, Ichiko Ikegami, Ryutaro Hanyu, Yuka Mitsuhashi Koike, Takuma Yamagishi, Genri Toyama, Aya Washida, Mari Tada, Akiyoshi Kakita, Osamu Onodera, Akihiro Sugai

FEBS Letters 2025.10

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Authorship：Lead author Publishing type：Research paper (scientific journal) Publisher：Wiley

Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are progressive neurodegenerative diseases characterised by nuclear TDP‐43 loss. Its hallmark, cryptic exon (CE) splicing, is often masked in bulk tissue analyses by the low abundance of affected neurons. We developed an ultrasensitive RT‐qPCR assay targeting STMN2 CE using one exon–CE junction‐spanning primer and the other within the CE. The design expands the dynamic range sevenfold: TDP‐43 knockdown boosted STMN2 CE levels 1395‐fold in differentiated SH‐SY5Y neurons. Spike‐in tests set detection at 0.16% deficient cells. Crucially, the assay revealed a 42‐fold CE increase in ALS motor cortex, previously missed by conventional primers. This streamlined tool enables precise quantification of TDP‐43 dysfunction and sensitive pharmacodynamic monitoring for future ALS‐FTD therapeutic studies.

Impact statement

Because cryptic‐exon signals are diluted in bulk tissue, we developed a junction‐spanning STMN2 RT‐qPCR with sub‐percent sensitivity. This deployable biomarker will aid ALS/FTD researchers and drug developers by standardizing measurements and enabling sensitive pharmacodynamic monitoring of therapies targeting nuclear TDP‐43 dysfunction.

DOI： 10.1002/1873-3468.70198

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The Multifaceted Regulation of TDP-43 Condensates at the Intersection of Physiology and Pathology: Implications for Neurodegenerative Diseases

Akihiro Sugai, Takuma Yamagishi, Shingo Koide, Osamu Onodera

Phase Separation in Living Cells 253 - 270 2023.9

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Publishing type：Part of collection (book) Publisher：Springer Nature Singapore

DOI： 10.1007/978-981-99-4886-4_13

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一側視野に限局した複雑幻視を呈した硬膜動静脈瘻の1例 Reviewed

小出眞悟, 畠山公大, 上村昌寛, 菊池文平, 長谷川仁, 小野寺理

臨床神経学 60 ( 6 ) 425 - 428 2020.6

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Authorship：Lead author Language：Japanese Publisher：(一社)日本神経学会

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Other Link： https://search.jamas.or.jp/default/link?pub_year=2020&ichushi_jid=J01550&link_issn=&doc_id=20200615340005&doc_link_id=1390848250133745152&url=https%3A%2F%2Fcir.nii.ac.jp%2Fcrid%2F1390848250133745152&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_3.gif
Role of RNF213 p.4810K variant in the development of intracranial arterial disease in patients treated with nilotinib. International journal

Masahiro Uemura, Masato Kanazawa, Takuma Yamagishi, Takahiro Nagai, Mami Takahashi, Shingo Koide, Masayoshi Tada, Junsuke Shimbo, Aiko Isami, Kunihiko Makino, Masayoshi Masuko, Kouji Nikkuni, Kouichirou Okamoto, Shuichi Igarashi, Kenichi Morita, Osamu Onodera

Journal of the neurological sciences 408 116577 - 116577 2020.1

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Language：English

DOI： 10.1016/j.jns.2019.116577

PubMed

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Glioblastoma mimicking limbic encephalitis Reviewed

Shingo Koide, Takao Fukushima, Hideki Mori, Gaku Ito, Daisuke Sato, Naoki Yajima, Toyotaka Aiba, Kunihiko Wakaki, Kunihiko Makino

Neurology and Clinical Neuroscience 2019.7

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Authorship：Lead author Publishing type：Research paper (scientific journal)

DOI： 10.1111/ncn3.12298

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MISC

【神経変性疾患の眼球運動異常】脊髄小脳変性症の眼球運動の特徴や異常

小出眞悟, 石原智彦, 小野寺理

神経眼科 40 ( 3 ) 222 - 228 2023.9

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Language：Japanese Publisher：日本神経眼科学会

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【いま新薬で加速する神経変性疾患研究異常タンパク質の構造、凝集のしくみから根本治療の真の標的に迫る】(第1章)タンパク質構造と病態 ALS病態のカギを握る液-液相分離

小出眞悟, 須貝章弘, 小野寺理

実験医学 41 ( 12 ) 1883 - 1889 2023.8

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Language：Japanese Publisher：(株)羊土社

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【ALS-どこまでわかり,どこまで治るか】原因と発症機序 TDP-43

小出眞悟, 須貝章弘, 小野寺理

Clinical Neuroscience 41 ( 3 ) 338 - 341 2023.3

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Language：Japanese Publisher：(株)中外医学社

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Presentations

新潟県,脊髄小脳変性疫学調査-臨床調査個人票による調査

小出眞悟, 本郷祥子, 秋山夏葵, 中村航世, 小野寺理

臨床神経学 2023.9 (一社)日本神経学会

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Event date： 2023.9

Language：Japanese

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日本人小脳失調症患者におけるRFC1のACAGG反復モチーフの拡大(Expansion of the ACAGG repeat motif in RFC1 in Japanese patients with cerebellar ataxia)

Koide Shingo, Ishihara Tomohiko, Hirokawa Sachiko, Sakakibara Satoko, Aiba Ikuko, Onodera Osamu

臨床神経学 2022.10 (一社)日本神経学会

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Event date： 2022.10

Language：English

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軟口蓋ミオクローヌスに対する下顎アプローチによる超音波検査

小出眞悟, 今野卓哉, 柏木健太, 柴田健太郎, 柳村文寛, 徳武孝允, 堅田慎一, 小野寺理

臨床神経学 2021.9 (一社)日本神経学会

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Event date： 2021.9

Language：Japanese

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進行性非流暢性失語(PNFA/nfvPPA)患者8例の臨床および画像的特徴の検討

小出眞悟, 他田正義, 羽入龍太郎, 高橋真実, 関谷可奈子, 佐藤晶, 五十嵐修一

臨床神経学 2020.11 (一社)日本神経学会

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Event date： 2020.11

Language：Japanese

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免疫抑制薬の減量が有効であった中枢神経原発移植後リンパ増殖性疾患の1例

小出眞悟, 徳武孝允, 茂木崇秀, 笠原壮, 中川由紀, 齊藤和英, 他田正義, 下畑享良, 小野寺理

神経治療学 2017.11 (一社)日本神経治療学会

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Event date： 2017.11

Language：Japanese

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リツキシマブが有効であったMAG抗体関連神経障害の67歳男性例

小出眞悟, 西田茉那, 斎藤奈つみ, 笠原壮, 二宮格, 堅田慎一, 小野寺理

臨床神経学 2016.12 (一社)日本神経学会

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Event date： 2016.12

Language：Japanese

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