Updated on 2022/12/05

写真a

 
HARA Takashi
 
Organization
Academic Assembly Institute of Science and Technology NOUGAKU KEIRETSU Associate Professor
Graduate School of Science and Technology Life and Food Sciences Associate Professor
Faculty of Agriculture Department of Agriculture Associate Professor
Title
Associate Professor
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Degree

  • 農学博士 ( 1999.3   九州大学 )

Research Interests

  • Cell Regulatory Technology

  • 食品機能学

  • Food Functionality

  • 細胞制御工学

Research Areas

  • Life Science / Food sciences

  • Life Science / Applied molecular and cellular biology

  • Life Science / Cell biology

Research History (researchmap)

  • Niigata University   Faculty of Agriculture

    2000 - 2002

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  • Research Associate, Faculty of Agriculture,

    2000 - 2002

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  • 日本学術振興会 特別研究員

    1998 - 2000

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  • Research Fellow of the Japan Society for the Promotion of Science

    1998 - 2000

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  • University of Niigata

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Research History

  • Niigata University   Faculty of Agriculture Department of Agriculture   Associate Professor

    2017.4

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2004.4

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2004.4

  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry   Associate Professor

    2004.4 - 2017.3

Education

  • Kyushu University   生物資源環境科学研究科   遺伝子資源工学

    - 1999

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    Country: Japan

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  • Kyushu University   Graduate School of Bioresouce and Bioenvironmental Sciences   Genetic Resources Technology

    - 1999

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  • Kyushu University   School of Agriculture   食糧化学工学科

    - 1994

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    Country: Japan

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  • Kyushu University   Faculty of Agriculture   Department of Food Science and Technology

    - 1994

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Professional Memberships

 

MISC

  • Purification and characterization of lipoxygenase from Pleurotus ostreatus

    T Kuribayashi, H Kaise, C Uno, T Hara, T Hayakawa, T Joh

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   50 ( 5 )   1247 - 1253   2002.2

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    Language:English   Publisher:AMER CHEMICAL SOC  

    Lipoxygenase was purified homogeneously from cups of Pleurotus ostreatus by Sephacryl S-400 HR gel filtration, Dyematrex Green A affinity, and DEAE-Toyopearl 650M ion-exchange chromatographies. The molecular weight of the enzyme was estimated to be 67 000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 66 000 by gel filtration; the isoelectric point was pH 5.1. The optimum pH and temperature of the enzymatic activity were 8.0 and 25 degreesC, respectively. The enzyme contained non-heme iron, and a thiol group seemed to be involved in its activity. The K-m, V-max and k(cat) values of the enzyme for linoleic acid were 0.13 mM, 23.4 mumol(.)min(-1.)mg(-1), and 25.7 s(-1), respectively. The enzyme showed high specificity toward linoleic acid. When linoleic acid was incubated with the enzyme, 13-hydroperoxy-9Z, 11E-octadecadienoic acid was found to be the main oxidative product.

    DOI: 10.1021/jf0112217

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  • Purification and characterization of lipoxygenase from Pleurotus ostreatus

    T Kuribayashi, H Kaise, C Uno, T Hara, T Hayakawa, T Joh

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   50 ( 5 )   1247 - 1253   2002.2

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    Language:English   Publisher:AMER CHEMICAL SOC  

    Lipoxygenase was purified homogeneously from cups of Pleurotus ostreatus by Sephacryl S-400 HR gel filtration, Dyematrex Green A affinity, and DEAE-Toyopearl 650M ion-exchange chromatographies. The molecular weight of the enzyme was estimated to be 67 000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 66 000 by gel filtration; the isoelectric point was pH 5.1. The optimum pH and temperature of the enzymatic activity were 8.0 and 25 degreesC, respectively. The enzyme contained non-heme iron, and a thiol group seemed to be involved in its activity. The K-m, V-max and k(cat) values of the enzyme for linoleic acid were 0.13 mM, 23.4 mumol(.)min(-1.)mg(-1), and 25.7 s(-1), respectively. The enzyme showed high specificity toward linoleic acid. When linoleic acid was incubated with the enzyme, 13-hydroperoxy-9Z, 11E-octadecadienoic acid was found to be the main oxidative product.

    DOI: 10.1021/jf0112217

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  • Formaction Mechanism of Grassy Odor Sabstance in Alfalfa Seedlings

    Food Science and Technology Research   8(4), 347-352   2002

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  • Formaction Mechanism of Grassy Odor Sabstance in Alfalfa Seedlings

    Food Science and Technology Research   8(4), 347-352   2002

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  • Gene Expression during Pi deficiency in Pholiota nameko:Accumulation of mRNA for Two Transporters

    Bioscience, Biotechnology, and Biochemistry   66(4), 790-800   2002

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  • Gene Expression during Pi deficiency in Pholiota nameko:Accumulation of mRNA for Two Transporters

    Bioscience, Biotechnology, and Biochemistry   66(4), 790-800   2002

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  • Increase in histamine content and enhancement of high affinity IgE receptor ε(epsilon) expression in the human leukemia KU812 cells upon treatment with hydrocortisone

    Cytotechnology   34 ( 3 )   213 - 223   2000

  • Enhancement of Fc epsilon RI expression by hydrocortisone induces the upregulation of Fc epsilon RI gamma mRNA in the human leukemia cell line KU812

    T Hara, H Tachibana, K Yamada

    ANIMAL CELL TECHNOLOGY: CHALLENGES FOR THE 21ST CENTURY   11   349 - 354   1999

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    Language:English   Publisher:SPRINGER  

    The human leukemia cell line KU812 have been described as an immature pre-basophilic cell line and exhibits a potential to differentiate into mature basophilic KU812 cells produce modest amount of histamine and express Fc epsilon RI, the high affinity IgE receptor, which plays a central role in the allergic responses system. Previously, we observed that hydrocortisone can enhance Fc epsilon RI expression in KU812 cells. In this study, we demonstrate that KU812 cells treated with hydrocortisone are able to release histamine in response to stimulation with IgE and anti-IgE antibody, suggesting that the Fc epsilon RI induced by hydrocortisone was functional enough to transduce a signal for degranulation. Fc epsilon RI is a tetramer consisting of an alpha chain, a beta chain and two gamma chains. We measured the mRNA level of each subunit in KU812 cells by RT-PCR followed by Southern blotting. After treatment with 1 mu M hydrocortisone for 35 days, the cells showed an increase in the amount of mRNA for Fc epsilon RI gamma. Almost no significant change in the amount of mRNA for Fc epsilon RI alpha and Fc epsilon RI beta was observed, This result suggests that the enhanced expression of Fc epsilon RI on the surface of KU812 cells by hydrocortisone was related to the upregulaion of Fc epsilon RI gamma mRNA.

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  • Effect of tea polyphenols on degranulation in human mature basophils differentiated with IL-4

    H Tachibana, Y Sunada, T Hara, K Yamada

    ANIMAL CELL TECHNOLOGY: CHALLENGES FOR THE 21ST CENTURY   11   301 - 305   1999

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    Language:English   Publisher:SPRINGER  

    We examined the effects of tea polyphenols on the activation process of human mature basophils by stimulation with Ca2+ ionophore A23187 or IgE receptor crosslinking. IL-4 was used to induce differentiation in human leukemia cell line KU812 into the morphological and functional human mature basophils used in this study. Among the tea polyphenols examined, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG) inhibited degranulation caused by IgE receptor crosslinking as well as by A23187. Thus, our findings suggest that the effects of ECG and EGCG may act on signaling pathway which regulates degranulation in human basophils after the increase of intracellular Ca2+ concentration.

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  • Basophilic differentiation of the human leukemia cell line KU812 upon treatment with interleukin-4

    T Hara, K Yamada, H Tachibana

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   247 ( 3 )   542 - 548   1998.6

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    Language:English   Publisher:ACADEMIC PRESS INC  

    The human leukemia cell line KU812 had been described as an immature prebasophilic cell line and exhibits a potential to differentiate into mature basophils. We studied the effect of interleukin-4 (IL-4) on the basophilic differentiation of KU812 cells. When KU812 cells were cultured with 1 ng/ml IL-4, cellular histamine content increased more than 10-fold. IL-4 also enhanced the expression of Fc epsilon RI, a high affinity IgE receptor, on the cell surface. KU812 cells treated with IL-4 expressed higher levels of Fc epsilon RI alpha, Fc epsilon RI beta and Fc epsilon RI gamma mRNA than non treated KU812 cells. After 21 days in culture with IL-4, KU812 cells became morphologically mature basophilic cells as demonstrated by staining positive for cytoplasmic granules and heparin proteoglycan by Wright dye and toluidin blue dye respectively. In addition, IgE-mediated histamine release was observed, suggesting that the Fc epsilon RI induced by IL-4 was functional and was able to transduce a signal for degranulation. These results suggest that IL-4 promotes differentiation of KU812 cells into mature basophilic cells both morphologically and functionally. (C) 1998 Academic Press.

    DOI: 10.1006/bbrc.1998.8816

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  • Hydrocortisone induces a formation of basophilic granules in the human leukemia cell line KU812

    T Hara, S Shirahata, H Tachibana

    ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 9   9   109 - 113   1998

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    Language:English   Publisher:SPRINGER  

    The human leukemia cell line KU812 exhibits a potential to differentiate into basophilic cells and has been described as a basophilic precursor cell line KU812 cells produce inflammatory mediators such as histamine and arachidonic acid metabolites. In addition, the cell line expresses high affinity IgE receptor (Fc epsilon RI) which is central to an allergic responses, but formation of cytoplasmic granules has not been accomplished When basophilic differentiation was induced in basophilic lineage cells, the synthesis of inflammatory mediators are increased and granulated cells are appeared in culture.
    Previously, we found that hydrocortisone can enhance the expression of Fc epsilon RI on KU812 cells. In this study, the effect of hydrocortisone on the formation of basophilic granules in KU812 cells was examined When the cells were cultured with 1 mu M hydrocortisone for 3 weeks, granulated cells that were stained metachromatically with Wright-Giemsa dye were observed In addition, the cellular histamine content of hydrocortisone-treated KU812 cells markedly increased after 3 weeks. These results suggest that hydrocortisone induces basophilic differentiation of KU812 cells.

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  • Induction of human leukemia cell line to differentiate into Fc epsilon RI expressing cells

    T Hara, H Tachibana, S Shirahata

    ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 8   8   565 - 570   1997

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    Language:English   Publisher:KLUWER ACADEMIC PUBL  

    We cultured human leukemia cell line KU812 to induce differentiation into basophil or mast cell. KU812 belongs to the myeloid cell lineage and is at least a bipotential cell that can differentiate into basophilic and monocyte/macrophage-like cells (1-3). When the KU812 cells were treated with recombinant human interleukin-3 (IL-3) or hydrocortisone (HC), we observed induction of differentiation (as measured morphologically and cytochemically) into monocyte/macrophage-like, neutrophilic and erythrocytic cells. Although basophilic granules were not detected on Wright-Giemsa stain, 5% of induced KU812 cells were stained positive with toluidine blue. Moreover, expression of high affinity IgE receptor Fc epsilon RI on the surface of KU812 cells was enhanced with IL-3 or HC. These results suggest that basophilic and mast cell-like cells present in cultures supplemented with IL-3 or HC.

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  • DEVELOPMENT OF A PROTEIN-FREE MEDIUM WITH IRON SALTS REPLACING TRANSFERRIN FOR A HUMAN-HUMAN HYBRIDOMA

    K NAGIRA, T HARA, M HAYASHIDA, K OSADA, M SHIGA, K SASAMOTO, K KINA, H MURAKAMI

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   59 ( 4 )   743 - 745   1995.4

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    Many protein-free media have been deveoped, because protein-free media are usually more economical than serum-free. or serum-containing media and facilitate the purification of bioactive materials, We evaluated various iron salts and chelating agents replacing transferrin to develop a protein-free medium for a human-human hybridoma, HB4C5, and found out that ferric citrate,vas favorable for the production and the productivity of monoclonal antibodies.

    DOI: 10.1271/bbb.59.743

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  • EFFECTS OF SODIUM POLY(STYRENE SULFONATE) ON ANTIBODY-PRODUCTION AND CELL-GROWTH OF HB4C5 HYBRIDOMA CELLS

    K NAGIRA, M HAYASHIDA, M SHIGA, K SASAMOTO, K KINA, K OSADA, T SUGAHARA, T HARA, Y YAMMOTO, H MURAKAMI

    POLYMER JOURNAL   27 ( 7 )   719 - 727   1995

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    Language:English   Publisher:SOC POLYMER SCIENCE JAPAN  

    The effects of sodium poly(styrene sulfonate) on an antibody production and cell growth of a hybridoma cell were studied. Although sodium poly(styrene sulfonate) inhibited the antibody production and cell growth at lower concentration with increasing molecular weight in serum-free cultures, sodium poly(styrene sulfonate)s with medium weight-average molecular weights (M(w)) such as 35000 and 18000 suppressed antibody production without inhibiting cell growth at appropriate concentrations. Sodium poly(styrene sulfonate) (M(w)=35000) decreased the extracellular concentration of a mu heavy chain forming an antibody, while it had no effect on an intracellular antibody or mu heavy chain concentration. This suggests that the sodium poly(styrene sulfonate) suppresses secreting the antibody assembled by mu heavy chains and lambda light chains out of the cell without inhibiting the synthesis and association of both chains in the cell.

    DOI: 10.1295/polymj.27.719

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Research Projects

  • Studies on attenuation of allergenicity of food components by ultra high presssure treatment.

    2002

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    Grant type:Competitive

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  • 超高圧処理による食品アレルゲンの低減化に関する研究

    2002

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    Grant type:Competitive

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  • Cellular Biology and Functional Analysis of Human Basophil and Mast Cell

    2000

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    Grant type:Competitive

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  • ヒト好塩基球およびマスト細胞の細胞生物学ならびに機能解析

    2000

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    Grant type:Competitive

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  • 食品成分および環境的化学物質の生体調節作用に関する研究

    2000

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    Grant type:Competitive

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  • Study on Bioregulatory Effects of Food Components and Environmental Chemical Substances.

    2000

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Teaching Experience

  • スタディ・スキルズAIIa

    2022
    Institution name:新潟大学

  • スタディ・スキルズAIa

    2022
    Institution name:新潟大学

  • Topics in Food Sciences

    2022
    Institution name:新潟大学

  • 食品科学プログラム実地見学

    2021
    Institution name:新潟大学

  • 食品機能化学特論

    2021
    Institution name:新潟大学

  • 免疫学概論

    2020
    Institution name:新潟大学

  • 食品科学概論

    2020
    Institution name:新潟大学

  • スタディ・スキルズA a

    2020
    Institution name:新潟大学

  • 食品安全学

    2019
    Institution name:新潟大学

  • 農学入門Ⅱ

    2019
    -
    2020
    Institution name:新潟大学

  • 農学入門Ⅰ

    2019
    -
    2020
    Institution name:新潟大学

  • 生命を知る

    2018
    Institution name:新潟大学

  • 基礎化学

    2017
    Institution name:新潟大学

  • 食と健康の科学

    2017
    Institution name:新潟大学

  • Topics in Food Sciences

    2016
    -
    2017
    Institution name:新潟大学

  • 応用生命・食品科学特論

    2014
    Institution name:新潟大学

  • 応用生命・食品科学概論

    2013
    -
    2016
    Institution name:新潟大学

  • 研究発表演習(中間発表)

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学セミナーBⅠ

    2012
    -
    2015
    Institution name:新潟大学

  • 文献詳読Ⅰ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学特定研究BⅡ

    2012
    -
    2015
    Institution name:新潟大学

  • 文献詳読Ⅱ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学特定研究BⅠ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学セミナーBⅡ

    2012
    -
    2015
    Institution name:新潟大学

  • 応用生命・食品科学演習(学会発表)

    2012
    Institution name:新潟大学

  • 食品・栄養科学演習Ⅰ

    2011
    -
    2019
    Institution name:新潟大学

  • 食品・栄養科学演習Ⅱ

    2011
    -
    2018
    Institution name:新潟大学

  • 応用生物化学概論

    2011
    Institution name:新潟大学

  • 食品・栄養科学実験

    2010
    -
    2018
    Institution name:新潟大学

  • 免疫・血清学

    2010
    -
    2018
    Institution name:新潟大学

  • 実地見学

    2010
    -
    2016
    Institution name:新潟大学

  • 生物化学実験

    2007
    Institution name:新潟大学

  • 食品分子機能学

    2007
    Institution name:新潟大学

  • 食品機能学

    2007
    Institution name:新潟大学

  • 食品機能化学特論

    2007
    -
    2018
    Institution name:新潟大学

  • くらしと微生物

    2007
    -
    2016
    Institution name:新潟大学

  • 生物学

    2007
    Institution name:新潟大学

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