Updated on 2025/10/14

写真a

 
HARADA Naoki
 
Organization
Academic Assembly Institute of Science and Technology NOUGAKU KEIRETSU Professor
Faculty of Agriculture Professor
Title
Professor
Other name(s)
Niigata University
External link

Degree

  • 博士(農学) ( 2001.3   東京大学 )

Research Areas

  • Environmental Science/Agriculture Science / Landscape science

  • Environmental Science/Agriculture Science / Environmental load reduction and remediation

  • Environmental Science/Agriculture Science / Environmental agriculture

  • Life Science / Plant nutrition and soil science

Research History (researchmap)

  • Niigata University   Graduate School of Science and Technology Specialized Course of Life and Food Sciences   Professor

    2018.4

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  • Niigata University   Faculty of Agriculture Department of Agriculture   Professor

    2018.4

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  • Niigata University   Faculty of Agriculture Department of Agriculture   Associate Professor

    2017.4 - 2018.3

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  • Niigata University   Graduate School of Science and Technology Specialized Course of Life and Food Sciences   Associate Professor

    2007.4 - 2018.3

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  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry   Associate Professor

    2007.4 - 2017.3

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Research History

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Professor

    2018.4

  • Niigata University   Faculty of Agriculture   Professor

    2018.4

  • Niigata University   Faculty of Agriculture Department of Agriculture   Associate Professor

    2017.4 - 2018.3

  • Niigata University   Associate Professor

    2007.4 - 2018.3

  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry   Associate Professor

    2007.4 - 2017.3

Professional Memberships

 

Papers

  • Trophic modulation of endophytes by rhizosphere protists

    Rasit Asiloglu, Seda Ozer Bodur, Solomon Oloruntoba Samuel, Murat Aycan, Jun Murase, Naoki Harada

    The ISME Journal   2024.11

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    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    The plant-microbe interactions, which is crucial for plant health and productivity, mainly occur in rhizosphere: a narrow zone of soil surrounding roots of living plants. The rhizosphere hosts one of the most intense habitats for microbial prey–predator interactions, especially between predatory protists and bacteria. Here, based on two key facts, microbial predators modulate rhizobacterial community composition, and the rhizobacterial community is the primary source of root microbiome, endophytes; we hypothesized that predation upon rhizobacteria would modulate the community composition of endophytic bacteria. The effects of three taxonomically distinct axenic protist species (Acanthamoeba castellanii, Vermamoeba vermiformis, and Heteromita globosa) were tested in this study. To examine the robustness of the hypotheses, the experiments were conducted in three soil types characterized by distinct bacterial communities and physicochemical properties. The bacterial community compositions were analyzed with high throughput sequencing. Bacterial gene abundances were estimated with a real-time-PCR method. The results showed that protists modulated endophytic communities, which originated in the rhizosphere soil. The modulation of endophytic communities by protists showed chaotic patterns rather than a deterministic effect under different soil types. The observed chaotic dynamics were further confirmed with an additional experiment, in which chaos was triggered by changes in the dilution rates of soil nutrients. Furthermore, the presence of predators enhanced the root colonization of endophytes. Our findings identify a key mechanism for the modulation of root endophytes and enhance understanding of underground plant-microbe interactions, which can lead to open new avenues for modulating the root microbiome to enhance crop production.

    DOI： 10.1093/ismejo/wrae235

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  • Differences in Early Root Endophytic Bacterial Communities between Japanese Sake Rice Cultivars and Table Rice Cultivars

    Sibel Sokel, Solomon Oloruntoba Samuel, Kazuki Suzuki, Naoki Harada

    Agronomy   14 ( 8 )   1769 - 1769   2024.8

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Sake, which is produced mainly from japonica rice (Oryza sativa subsp. japonica), is one of the most important alcohol products in Japan. In this study, we aimed to investigate a hypothesis that the early root endophytic bacterial communities in Japanese sake rice cultivars would be distinct from those in table rice cultivars, comparing four sake rice cultivars and two table rice cultivars. Rice roots in the vegetative stage were collected 0, 3, and 6 weeks after transplanting, and 16S rRNA gene amplicon sequencing revealed significant differences in bacterial community composition diversity between the sake and table rice cultivars. The root endophytic bacterial communities at the transplanting differed significantly between the rice cultivars, indicating differences in each seed-derived endophytic community. After an overall dominance of Pantoea and Methylobacterium-Methylorubrum at the transplanting, the endophytic community was gradually replaced by soil-derived bacteria that varied by the rice cultivars. Notably, PERMANOVA results showed that the rice endophytic bacterial community composition differed significantly between the sake and table rice cultivars (p < 0.001). These results highlight the distinct root endophytic bacterial composition in the sake rice cultivars compared to those in the table rice cultivars, supporting our hypothesis.

    DOI： 10.3390/agronomy14081769

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  • Guardians of plant health: roles of predatory protists in the pathogen suppression Reviewed

    Mayu Fujino, Seda Ozer Bodur, Naoki Harada, Rasit Asiloglu

    Plant and Soil   2024.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s11104-024-06846-w

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    Other Link： https://link.springer.com/article/10.1007/s11104-024-06846-w/fulltext.html

  • Nitrogen-based fertilizers differentially affect protist community composition in paddy field soils Reviewed

    Seda Ozer Bodur, Solomon Oloruntoba Samuel, Kazuki Suzuki, Naoki Harada, Rasit Asiloglu

    Soil Ecology Letters   6 ( 3 )   2024.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s42832-023-0221-3

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    Other Link： https://link.springer.com/article/10.1007/s42832-023-0221-3/fulltext.html

  • The Application of Organic Matter Temporarily Shifts Carrot Prokaryotic Communities in the Endosphere but Not in the Rhizosphere Reviewed

    Irem Bagci, Kazuki Suzuki, Rasit Asiloglu, Naoki Harada

    Microorganisms   11 ( 10 )   2377 - 2377   2023.9

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    Authorship：Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Endophytic prokaryotes, bacteria, and archaea, are important microorganisms that benefit host plants by promoting plant growth and reducing stress. The objective of this study was to evaluate temporal shifts in the root endophytic prokaryotic communities associated with carrots (Daucus carota subsp. sativus) and the effect of organic matter application on them. Carrots were grown in a planter under five fertilizer treatments (weed compost, bark compost, cattle manure, chemical fertilizer, and no-fertilizer control) and the compositions of rhizosphere and root endosphere prokaryotic communities were determined via amplicon sequencing analysis targeting the 16S rRNA gene at 60 and 108 days after sowing. The results showed that the rhizosphere prokaryotic community compositions were stable despite different sampling times and fertilizer treatments; however, a greater temporal shift and an effect of the type of organic matter applied were observed in the endosphere prokaryotic communities. The differences in treatments resulted in significant differences in the abundance and Faith pyrogenetic diversity of the endosphere prokaryotic community. Genera, such as Burkholderia, Sphingomonas, and Rhodanobacter, that exhibit plant-growth-promoting and biocontrol activities, were detected regardless of the treatments, suggesting that they may play an important ecological role as the core endophytes in carrot roots.

    DOI： 10.3390/microorganisms11102377

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  • Bacterial Community Composition Under Paddy Conditions Is More Strongly Affected by the Difference in Soil Type than by Field Management Reviewed

    Kazuki Suzuki, Kana Katashima, Takaaki Miki, Hajime Igarashi, Qicong Xu, Shinji Ohkubo, Shinji Iwaishi, Naoki Harada

    Microbial Ecology   2023.7

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    Authorship：Last author   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00248-023-02261-3

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    Other Link： https://link.springer.com/article/10.1007/s00248-023-02261-3/fulltext.html

  • Rice endophytic communities are strongly dependent on microbial communities specific to each soil Reviewed

    Solomon Oloruntoba Samuel, Kazuki Suzuki, Rasit Asiloglu, Naoki Harada

    Biology and Fertility of Soils   59 ( 7 )   733 - 746   2023.5

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00374-023-01743-3

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    Other Link： https://link.springer.com/article/10.1007/s00374-023-01743-3/fulltext.html

  • Protists modulate active bacterial community composition in paddy field soils Reviewed

    Mayu Fujino, Kazuki Suzuki, Naoki Harada, Rasit Asiloglu

    Biology and Fertility of Soils   Accepted ( 7 )   709 - 721   2023.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00374-023-01733-5

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    Other Link： https://link.springer.com/article/10.1007/s00374-023-01733-5/fulltext.html

  • Soil 137Cs Activity Concentrations in Upland fields after Decontamination and Its Transfer to Soybeans Reviewed

    Naoki HARADA, Saki ITOH, Yusuke KOWATA, Yoshimasa SUZUKI, Masanori NONAKA

    Journal of Reconstruction Agriculture and Sciences   3 ( 1 )   1 - 10   2023.1

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    Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

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  • A deep placement of lime nitrogen reduces the nitrate leaching and promotes soybean growth and seed yield Reviewed

    Takuji Ohyama, Keisuke Ikebe, Sotaro Okuoka, Tomoya Ozawa, Takuya Nishiura, Taiga Ishiwata, Ayane Yamazaki, Fumu Tanaka, Toru Takahashi, Takumi Umezawa, Hiroyuki Ohshima, Taku Kato, Yoshiyuki Maeda, Akihiro Saito, Kyoko Higuchi, Norikuni Ohtake, Yoshihiko Takahashi, Naoki Harada, Naoko Ohkama-Ohtsu

    Crop and Environment   1 ( 4 )   221 - 230   2022.12

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.crope.2022.09.002

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  • Investigation on the radiocesium transfer to rice plants near the water inlet of paddy fields via an in situ experiment using non-contaminated soil Reviewed

    Anastasiia Klevtsova, Rinji Inaba, Moeka Takahashi, Yoshimasa Suzuki, Susumu Miyazu, Kazuki Suzuki, Naoki Harada, Norio Nogawa, Tatsuhiro Nishikiori, Tomijiro Kubota, Natsuki Yoshikawa

    JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY   2022.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10967-022-08448-1

    Web of Science

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  • Improvement in potassium budget with water outflow reduction through reducing excessive irrigation in a paddy field Reviewed

    Tatsuhiro Nishikiori, Tomijiro Kubota, Susumu Miyazu, Naoki Harada, Natsuki Yoshikawa

    SOIL SCIENCE AND PLANT NUTRITION   2022.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/00380768.2022.2108685

    Web of Science

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  • Elevated rice 137Cs concentrations near the water inlet in paddy fields after the Fukushima nuclear accident Reviewed

    Yoshimasa Suzuki, Ryosuke Shoji, Takahiro Tsurumaki, Syohei Tamaki, Kousei Nakashima, Susumu Miyazu, Natsuki Yoshikawa, Hideki Ishii, Norio Nogawa, Masanori Nonaka, Kazuki Suzuki, Naoki Harada

    Journal of Radioanalytical and Nuclear Chemistry   331 ( 7 )   3167 - 3173   2022.7

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    Authorship：Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s10967-022-08350-w

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    Other Link： https://link.springer.com/article/10.1007/s10967-022-08350-w/fulltext.html

  • Microplastic Extraction from Agricultural Soils Using Canola Oil and Unsaturated Sodium Chloride Solution and Evaluation by Incineration Method Reviewed

    Andrei Kononov, Motoya Hishida, Kazuki Suzuki, Naoki Harada

    Soil Systems   6 ( 2 )   54 - 54   2022.6

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    Authorship：Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Environmental pollution by microplastics (MPs) has become a global problem, but little is known about MPs in soils. This is because MP extraction methods from soils have not yet been standardized. In this study, we tried to establish a simple and economical method to extract soil MPs using the buoyancy of canola oil and the density separation process using sodium chloride (NaCl). In addition, the incineration method was adapted to evaluate the effectiveness of extraction methods precisely. First, the ability and suitability of seven different oils to extract MP from soil were investigated and canola oil was selected. Then, the spiking and recovery test was performed with canola oil and NaCl solution for low-density polyethylene (LDPE), polypropylene (PP), and polyvinylchloride (PVC) as follows: (1) soil and MP mixtures were prepared, (2) 5 g L−1 NaCl and canola oil were added and shaken thoroughly, (3) the oil phase containing MPs were separated after sedimentation, (4) the extracted MPs were rinsed with 99.5% ethanol, and (5) the organic adherents to the extracted MPs were digested with hydrogen peroxide. After drying and incineration, the substantial recovery rates were calculated. In the spiking and recovery test for MP particles (<1 mm) from five typical Japanese agricultural soils, the recoveries of LDPE, PP, and PVC were 95.2–98.3%, 95.2–98.7%, and 76.0–80.5%, respectively, higher than those obtained by the density separation using saturated NaCl solution. In conclusion, the method is effective for extracting MPs, especially LDPE and PP, from soils and is less sensitive to soil type, texture, and physicochemical properties.

    DOI： 10.3390/soilsystems6020054

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  • Impact of the chemical composition of applied organic materials on bacterial and archaeal community compositions in paddy soil Reviewed

    Sharmin Akter Chowdhury, Aya Kaneko, Md Zakaria Ibne Baki, Chikako Takasugi, Natsumi Wada, Rasit Asiloglu, Naoki Harada, Kazuki Suzuki

    Biology and Fertility of Soils   58 ( 2 )   135 - 148   2022.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00374-022-01619-y

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    Other Link： https://link.springer.com/article/10.1007/s00374-022-01619-y/fulltext.html

  • Suppressive effect of the deep placement of lime nitrogen on N2O emissions in a soybean field Reviewed

    Hiromu Ikezawa, Yoshifumi Nagumo, Makoto Hattori, Masanori Nonaka, Takuji Ohyama, Naoki Harada

    Science of The Total Environment   804   150246 - 150246   2022.1

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.scitotenv.2021.150246

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  • Soil-root interface influences the assembly of the endophytic bacterial community in rice plants Reviewed

    Solomon Oloruntoba Samuel, Kazuki Suzuki, Rasit Asiloglu, Naoki Harada

    Biology and Fertility of Soils   58 ( 1 )   35 - 48   2021.11

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00374-021-01611-y

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    Other Link： https://link.springer.com/article/10.1007/s00374-021-01611-y/fulltext.html

  • Soil properties have more significant effects on the community composition of protists than the rhizosphere effect of rice plants in alkaline paddy field soils Reviewed

    Rasit Asiloglu, Keiko Shiroishi, Kazuki Suzuki, Oguz Can Turgay, Naoki Harada

    Soil Biology and Biochemistry   161   108397 - 108397   2021.10

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.soilbio.2021.108397

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  • Molecular Genetic Characterization of Arbuscular Mycorrhizal Fungi Associated with Upland Rice in Bangladesh Reviewed

    Md Zakaria Ibne Baki, Kazuki Suzuki, Kohei Takahashi, Sharmin Akter Chowdhury, Rasit Asiloglu, Naoki Harada

    Rhizosphere   18   100357 - 100357   2021.6

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.rhisph.2021.100357

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  • Top-down effects of protists are greater than bottom-up effects of fertilisers on the formation of bacterial communities in a paddy field soil Reviewed

    Rasit Asiloglu, Kobayashi Kenya, Solomon Oloruntoba Samuel, Bahar Sevilir, Jun Murase, Kazuki Suzuki, Naoki Harada

    Soil Biology and Biochemistry   156   108186 - 108186   2021.5

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.soilbio.2021.108186

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  • Effect of protists on rhizobacterial community composition and rice plant growth in a biochar amended soil Reviewed

    Rasit Asiloglu, Bahar Sevilir, Solomon Oloruntoba Samuel, Murat Aycan, Muhittin Onur Akca, Kazuki Suzuki, Jun Murase, Oguz Can Turgay, Naoki Harada

    Biology and Fertility of Soils   57 ( 2 )   293 - 304   2021.2

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00374-020-01525-1

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    Other Link： http://link.springer.com/article/10.1007/s00374-020-01525-1/fulltext.html

  • Impacts of application of calcium cyanamide and the consequent increase in soil pH on N2O emissions and soil bacterial community compositions Reviewed

    Kazuki Suzuki, Naoya Kashiwa, Kota Nomura, Rasit Asiloglu, Naoki Harada

    Biology and Fertility of Soils   57 ( 2 )   269 - 279   2021.2

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00374-020-01523-3

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    Other Link： http://link.springer.com/article/10.1007/s00374-020-01523-3/fulltext.html

  • Biochar affects taxonomic and functional community composition of protists Reviewed

    Rasit Asiloglu, Solomon Oloruntoba Samuel, Bahar Sevilir, Muhittin Onur Akca, Pinar Acar Bozkurt, Kazuki Suzuki, Jun Murase, Oguz Can Turgay, Naoki Harada

    Biology and Fertility of Soils   57 ( 1 )   15 - 29   2021.1

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    Authorship：Last author   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00374-020-01502-8

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    Other Link： http://link.springer.com/article/10.1007/s00374-020-01502-8/fulltext.html

  • Influence of irrigation water intake on local increase of radiocesium activity concentration in rice plants near a water inlet Reviewed International journal

    Natsuki Yoshikawa, Kosei Nakashima, Yoshimasa Suzuki, Susumu Miyazu, Kazuki Suzuki, Norio Nogawa, Naoki Harada

    Journal of Environmental Radioactivity   225   106441 - 106441   2020.12

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    To identify the cause of the phenomenon that rice plants close to the water inlet contain relatively higher radiocesium within a paddy field plot, we conducted a field experiment by establishing experimental channel using polypropylene corrugated sheets, and sampling surface water, paddy soil and rice plants according to the distance from the water inlet in 2014 and 2015. It was found that the 137Cs activity concentrations in both dissolved and particulate forms in paddy surface water presented a declining trend from the water inlet towards the outlet. The 137Cs activity concentration in paddy soil in the harvesting season and those of brown rice and rice straws were highest at 1-2 m from the water inlet. Balance calculation suggests that destination of the lost 137Cs from the surface water was likely to be adsorption of the dissolved form and sedimentation of particulate form onto the soil. The concentration of exchangeable potassium ion in paddy soil was below the recommended standard of 250 mg kg-1 (as K2O in dry soil) near the water inlet at the harvesting period both years．These findings suggested that the possible crucial factors to induce rice plant uptake of radiocesium near the water inlet were either (1) direct absorption of dissolved 137Cs in surface water by rice plants, (2) absorption of 137Cs, which was originally retained in particulate matter and released by ion exchange and/or by organic matter decomposition in combination with (3) loss of soil exchangeable potassium caused due partly to transportation of soil particles with exchangeable potassium by the rapid water flow near the water inlet and/or leaching by ion exchange onto the soil of other cations such as calcium ion flowing into the paddy field. These findings will contribute to providing possible measures for producing safe rice in highly contaminated areas in which agricultural production will resume in the near future. We propose providing a non-planting zone for the area closer than about 5 m from the water intake to avoid the occurrence of high 137Cs concentrations in rice crops.

    DOI： 10.1016/j.jenvrad.2020.106441

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  • Protist-enhanced survival of a plant growth promoting rhizobacteria, Azospirillum sp. B510, and the growth of rice (Oryza sativa L.) plants Reviewed

    Asiloglu R, Shiroishi K, Suzuki K, Turgay OC, Murase J, Harada N

    Applied Soil Ecology   154   103599 - 103599   2020.10

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.apsoil.2020.103599

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  • The composition characteristics of arbuscular mycorrhizal fungal communities associated with barley in saline-alkaline soils in Central Anatolia Reviewed

    Kaidzu T, Suzuki K, Sugiyama H, Akca MO, Ergül A, Turgay OC, Nonaka M, Harada N

    Soil Science and Plant Nutrition   66 ( 2 )   268 - 274   2020.4

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  • Input and output pathways determining potassium budgets of two paddy fields implementing countermeasures against radiocesium in Fukushima, Japan Reviewed

    Nishikiori T, Kubota T, Miyazu S, Harada N, Yoshikawa N, Fujiwara H, Saito T

    Plos one   15 ( 4 )   e0232139   2020.4

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  • Molecular characterization, mycelial compatibility grouping, and aggressiveness of a newly emerging phytopathogen, Sclerotinia sclerotiorum, causing white mold disease in new host crops in Bangladesh Reviewed

    Rahman MME, Suzuki K, Islam MM, Dey TK, Harada N, Hossain DM

    Journal of Plant Pathology   102 ( 3 )   775 - 785   2020.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s42161-020-00503-8

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    Other Link： http://link.springer.com/article/10.1007/s42161-020-00503-8/fulltext.html

  • Evaluation of primer pairs for studying arbuscular mycorrhizal fungal community compositions using an MiSeq platform Reviewed

    Suzuki K, Takahashi K, Harada N

    Biology Fertility of Soils   2020.4

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  • P8-1-8 天水田における作土中<sup>137</sup>Csの滞留半減時間の推定（8-1 環境保全，2019年静岡大会）

    原田 直樹, 鈴木 一輝, 宮津 進, 吉川 夏樹

    日本土壌肥料学会講演要旨集   65   135 - 135   2019.9

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    Language：Japanese   Publishing type：Research paper (conference, symposium, etc.)   Publisher：一般社団法人 日本土壌肥料学会  

    DOI： 10.20710/dohikouen.65.0_135_2

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  • Salt tolerance improvement in rice through efficient SNP marker-assisted selection coupled with speed-breeding Reviewed International journal

    Md Masud Rana, Takeshi Takamatsu, Marouane Baslam, Kentaro Kaneko, Kimiko Itoh, Naoki Harada, Toshie Sugiyama, Takayuki Ohnishi, Tetsu Kinoshita, Hiroki Takagi, Toshiaki Mitsui

    International journal of molecular sciences   20 ( 10 )   2585   2019.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    Salinity critically limits rice metabolism, growth, and productivity worldwide. Improvement of the salt resistance of locally grown high-yielding cultivars is a slow process. The objective of this study was to develop a new salt-tolerant rice germplasm using speed-breeding. Here, we precisely introgressed the hst1 gene, transferring salinity tolerance from "Kaijin" into high-yielding "Yukinko-mai" (WT) rice through single nucleotide polymorphism (SNP) marker-assisted selection. Using a biotron speed-breeding technique, we developed a BC3F3 population, named "YNU31-2-4", in six generations and 17 months. High-resolution genotyping by whole-genome sequencing revealed that the BC3F2 genome had 93.5% similarity to the WT and fixed only 2.7% of donor parent alleles. Functional annotation of BC3F2 variants along with field assessment data indicated that "YNU31-2-4" plants carrying the hst1 gene had similar agronomic traits to the WT under normal growth condition. "YNU31-2-4" seedlings subjected to salt stress (125 mM NaCl) had a significantly higher survival rate and increased shoot and root biomasses than the WT. At the tissue level, quantitative and electron probe microanalyzer studies indicated that "YNU31-2-4" seedlings avoided Na+ accumulation in shoots under salt stress. The "YNU31-2-4" plants showed an improved phenotype with significantly higher net CO2 assimilation and lower yield decline than WT under salt stress at the reproductive stage. "YNU31-2-4" is a potential candidate for a new rice cultivar that is highly tolerant to salt stress at the seedling and reproductive stages, and which might maintain yields under a changing global climate.

    DOI： 10.3390/ijms20102585

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  • Differences in soil bacterial community compositions in paddy fields under organic and conventional farming conditions Reviewed

    Kazuki Suzuki, Manami Takemura, Takaaki Miki, Masanori Nonaka, Naoki Harada

    Microbes and Environments   34 ( 1 )   108 - 111   2019.3

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    Soil bacterial community compositions and temporal changes in organic paddy fields were elucidated using a 16S rRNA gene amplicon sequencing analysis with a high-throughput next generation sequencer. At transplanting, bacterial community compositions in organic and conventional paddy fields were mostly similar despite differences in field management. The bacterial community composition in organic fields differed from that under conventional management during the rice growth period, possibly as a result of the decomposition process of organic fertilizers. However, differences in the frequency of tillage and photosynthetic bacterial inoculations in the organic plots had less of an impact on bacterial communities.

    DOI： 10.1264/jsme2.ME18101

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  • Yearly changes in rice and soil radiocaesium concentrations in Minamisoma City, Fukushima Prefecture, Japan —Monitoring results from 2013 to 2016— Reviewed

    Suzuki Y, Shoji R, Tsurumaki T, Matsubara T, Tamaki S, Nakajima K, Tsuruta R, Yoshikawa N, Ishii H, Nogawa N, Nonaka M, Harada N

    Radioisotopes   68 ( 1 )   1 - 12   2019.1

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    Authorship：Last author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

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  • Biological control of tomato collar rot induced by Sclerotium rolfsii using Trichoderma species isolated in Bangladesh Reviewed

    Md. Monirul Islam, Delwar M. Hossain, Masanori Nonaka, Naoki Harada

    Archives of Phytopathology and Plant Protection   50 ( 3-4 )   109 - 116   2017.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Taylor and Francis Ltd.  

    DOI： 10.1080/03235408.2016.1265243

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  • Measurement and estimation of radiocesium discharge rate from paddy field during land preparation and mid-summer drainage Reviewed

    Susumu Miyazu, Tetsuo Yasutaka, Natsuki Yoshikawa, Shouhei Tamaki, Kousei Nakajima, Iku Sato, Masanori Nonaka, Naoki Harada

    JOURNAL OF ENVIRONMENTAL RADIOACTIVITY   155   23 - 30   2016.5

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    DOI： 10.1016/j.jenvrad.2016.01.013

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  • 4,4'-DDE and Endosulfan Levels in Agricultural Soils of the Cukurova Region, Mediterranean Turkey Reviewed

    Muhittin Onur Akca, Shihoko Hisatomi, Manami Takemura, Naoki Harada, Masanori Nonaka, Futa Sakakibara, Kazuhiro Takagi, Oguz Can Turgay

    BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY   96 ( 3 )   376 - 382   2016.3

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    DOI： 10.1007/s00128-015-1714-2

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  • Native Trichoderma strains isolated from Bangladesh with broad spectrum antifungal action against fungal phytopathogens Reviewed

    Md. Monirul Islam, Delwar M. Hossain, Md. Muzahid E Rahman, Kazuki Suzuki, Taishi Narisawa, Ismail Hossain, Md. Bahadur Meah, Masanori Nonaka, Naoki Harada

    Archives of Phytopathology and Plant Protection   49 ( 1-4 )   75 - 93   2016.2

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    DOI： 10.1080/03235408.2016.1147121

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  • Suppressive effects of Bacillus spp. on mycelia, apothecia and sclerotia formation of Sclerotinia sclerotiorum and potential as biological control of white mold on mustard Reviewed

    Md Muzahid E. Rahman, Delwar M. Hossain, Kazuki Suzuki, Ayaka Shiiya, Kazushi Suzuki, Tapan Kumar Dey, Masanori Nonaka, Naoki Harada

    AUSTRALASIAN PLANT PATHOLOGY   45 ( 1 )   103 - 117   2016.2

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    DOI： 10.1007/s13313-016-0397-4

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  • Arbuscular mycorrhizal fungal community of wheat under long-term mineral and organic amendments in semi-arid Mediterranean Turkey Reviewed

    Takuya Buto, Kazuki Suzuki, Tomoyuki Kaidzu, Taishi Narisawa, Oguz Can Turgay, Ibrahim Ortas, Naoki Harada, Masanori Nonaka

    ARID LAND RESEARCH AND MANAGEMENT   30 ( 4 )   479 - 489   2016

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    DOI： 10.1080/15324982.2016.1177749

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  • First Report of white mold on Jackfruit caused by Sclerotinia sclerotiorum in Bangladesh Reviewed

    Md Muzahid-E- Rahman, Tapan Kumar Dey, Delwar M Hossain, Masanori Nonaka, Naoki Harada

    Australasian Plant Disease Notes   10 ( 10 )   2015.12

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    DOI： 10.1007/s13314-014-0155-9

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  • Radiocesium contamination of soybean (Glycine max) produced with no fertilization in 2014: A case study in Minamisoma City, Fukushima Prefecture, Japan Reviewed

    Naoki Harada, Saki Ito, Naoto Nihei, Masanori Nonaka

    Japanese Journal of Organic Agriculture Science   7 ( 2 )   35 - 41   2015.12

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  • <sup>137</sup>Cs Distribution in Mulberry(Morus alba) after the Fukushima Dai-ichi Nuclear Power Plant Accident and Effect of Spray Application of a Liquid Potassium Fertilizer onto Trunk Surface Reviewed

    HARADA Naoki, MOTOJIMA Sayaka, IGARASHI Kazuki, NONAKA Masanori

    RADIOISOTOPES   64 ( 10 )   613 - 619   2015.10

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    <i>After the Fukushima Dai‐ichi Nuclear Power Plant accident in March 2011, contamination of mulberry-leaf tea with over 100</i>Bq kg<sup>－1</sup> <sup>137</sup>Cs<i> was found. We therefore investigated </i><sup>137</sup>Cs<i> distribution in mulberry trees(Morus alba) and soil in Nihonmatsu City from 2012. As a result, mulberry leaves near the end of branches(0 - 30</i>cm<i>), which are usually used as edible leaves, were shown to contain higher </i><sup>137</sup>Cs<i> concentration than the others. Trunk showed highest </i><sup>137</sup>Cs<i> concentration among the plant parts of mulberry investigated, while root </i><sup>137</sup>Cs<i> concentration was relatively low. Autoradiographic studies for leaf, bark and cross-sectional trunk suggest that radioactive substances were deposited onto bark and leaf radioactive contamination could be due to translocation of </i><sup>137</sup>Cs<i> from trunk. Spray application of 0.5</i>% KH<sub>2</sub>PO<sub>4</sub><i> solution onto trunk surface significantly reduced </i><sup>137</sup>Cs<i> concentration in edible leaves emerged about one month after the application. However, the effect was limited and insufficient to solve the problem of radioactive contamination in edible mulberry leaves.</i>

    DOI： 10.3769/radioisotopes.64.613

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    Other Link： https://jlc.jst.go.jp/DN/JLC/20016766325?from=CiNii

  • FIRST REPORT OF WHITE MOULD CAUSED BY SCLEROTINIA SCLEROTIORUM ON MARIGOLD (TAGETES ERECTA) IN BANGLADESH Reviewed

    M. M. E. Rahman, D. M. Hossain, T. K. Dey, S. R. Sarker, M. Nonaka, N. Harada

    JOURNAL OF PLANT PATHOLOGY   97 ( 2 )   398 - 398   2015.7

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    DOI： 10.4454/JPP.V97I2.046

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  • Sulfate-reducing bacteria mediate thionation of diphenylarsinic acid under anaerobic conditions Reviewed

    Ling Guan, Ayaka Shiiya, Shihoko Hisatomi, Kunihiko Fujii, Masanori Nonaka, Naoki Harada

    BIODEGRADATION   26 ( 1 )   29 - 38   2015.2

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    DOI： 10.1007/s10532-014-9713-2

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  • First report of white mould caused by Sclerotinia sclerotiorum on jackfruit Reviewed

    Md Muzahid E Rahman, Tapan Kumar Dey, Delwar M. Hossain, Masanori Nonaka, Naoki Harada

    Australasian Plant Disease Notes   10 ( 1 )   1 - 3   2015

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    DOI： 10.1007/s13314-014-0155-9

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  • 地中海沿岸地域における長期有機連用がトウモロコシ根の糸状菌叢に与える影響(2015年度大会講演要旨)

    鈴木 一輝, 戸井田 千佳, 原田 直樹, 野中 昌法

    土と微生物   69 ( 2 )   128   2015

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    DOI： 10.18946/jssm.69.2_128_2

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  • 次世代シーケンサーを用いた土壌細菌群集構造解析による有機水田土壌のプロファイリングの試み(2015年度大会講演要旨)

    竹村 真奈美, 鈴木 一輝, 野中 昌法, 原田 直樹

    土と微生物   69 ( 2 )   125   2015

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    DOI： 10.18946/jssm.69.2_125_2

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  • Molecular diversity of indigenous arbuscular mycorrhizal fungi in three different agricultural regions of Turkey Reviewed

    Kazuki Suzuki, Oguz Can Turgay, Muhittin Onur Akca, Naoki Harada, Masanori Nonaka

    SOIL SCIENCE AND PLANT NUTRITION   60 ( 3 )   367 - 376   2014.6

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    DOI： 10.1080/00380768.2014.890916

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  • Cs-137 in irrigation water and its effect on paddy fields in Japan after the Fukushima nuclear accident Reviewed

    Natsuki Yoshikawa, Hitomi Obara, Marie Ogasa, Susumu Miyazu, Naoki Harada, Masanori Nonaka

    SCIENCE OF THE TOTAL ENVIRONMENT   481   252 - 259   2014.5

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    DOI： 10.1016/j.scitotenv.2014.01.129

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  • Formation of diphenylthioarsinic acid from diphenylarsinic acid under anaerobic sulfate-reducing soil conditions Reviewed

    Shihoko Hisatomi, Ling Guan, Mami Nakajima, Kunihiko Fujii, Masanori Nonaka, Naoki Harada

    Journal of Hazardous Materials   262   25 - 30   2013.11

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    DOI： 10.1016/j.jhazmat.2013.08.020

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  • Effects of diphenylarsinic acid on bacterial and archaeal community structures in an anaerobic paddy soil Reviewed

    Ling Guan, Naoki Harada, Yasushi Ono, Takuya Takahashi, Kunihiko Fujii, Xiaolan Liu, Masanori Nonaka

    GEODERMA   195   243 - 250   2013.3

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    DOI： 10.1016/j.geoderma.2012.12.007

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  • Radioactive Cesium Decontamination and Strategic Farmland Resource Conservation Reviewed

    ARITA Hiroyuki, HASHIMOTO Shizuka, YOSHIKAWA Natsuki, HARADA Naoki, YASUTAKA Tetsuo, NONAKA Masanori, IWASAKI Yumi, MIYAZU Susumu

    Transactions of The Japanese Society of Irrigation, Drainage and Rural Engineering   80 ( 6 )   555 - 561   2012.12

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    Radiocesium (Cs) decontamination of land has been required as one of the restoration measures after the Tohoku-Pacific Ocean Earthquake disaster. The Ministry of Agriculture, Forestry and Fisheries (MAFF) of Japan plans to carry out decontamination measures of farmland in response to the situation of land contamination. However, in order to achieve effective restoration, strategic and comprehensive measures including land readjustment are necessary. In this paper, we examined characteristics of Cs contamination from the view of the spatial extent contamination, land use, land improvement and working environment, and made the following proposals: (1) Implementation of Cs decontamination works ought to follow the principle of &ldquo;containment&rdquo;, &ldquo;isolation&rdquo;, and &ldquo;securing long-term safety&rdquo; of Cs, and improve both production environment and working environment of farmlands. (2) Strategic approaches should be carried out in a comprehensive and unified manner for the effective implementation of Cs decontamination.

    DOI： 10.11408/jsidre.80.555

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  • Enhanced transformation of diphenylarsinic acid in soil under sulfate-reducing conditions Reviewed

    Ling Guan, Shihoko Hisatomi, Kunihiko Fujii, Masanori Nonaka, Naoki Harada

    JOURNAL OF HAZARDOUS MATERIALS   241   355 - 362   2012.11

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    DOI： 10.1016/j.jhazmat.2012.09.054

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  • Soil radiocesium distribution in rice fields disturbed by farming process after the Fukushima Dai-ichi Nuclear Power Plant accident Reviewed

    Naoki Harada, Masanori Nonaka

    SCIENCE OF THE TOTAL ENVIRONMENT   438   242 - 247   2012.11

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    DOI： 10.1016/j.scitotenv.2012.05.032

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  • Biodegradation of melamine and its hydroxy derivatives by a bacterial consortium containing a novel Nocardioides species Reviewed

    Kazuhiro Takagi, Kunihiko Fujii, Ken-ichi Yamazaki, Naoki Harada, Akio Iwasaki

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   94 ( 6 )   1647 - 1656   2012.6

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    DOI： 10.1007/s00253-011-3673-9

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  • Biodegradation of diphenylarsinic acid to arsenic acid by novel soil bacteria isolated from contaminated soil Reviewed

    Naoki Harada, Kazuhiro Takagi, Koji Baba, Kunihiko Fujii, Akio Iwasaki

    BIODEGRADATION   21 ( 3 )   491 - 499   2010.6

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    DOI： 10.1007/s10532-009-9318-3

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  • Characteristics of nitrogen dynamics in organic glasshouse tomato in Niigata Prefecture Reviewed

    Masanori NONAKA, Yui FUJINO, Naoki HARADA

    Japanese Journal of Organi Agriculture Science   1 ( 1 )   42 - 51   2009.12

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  • Aerobic Mineralization of Hexachlorobenzene by Newly Isolated Pentachloronitrobenzene-Degrading Nocardioides sp Strain PD653 Reviewed

    Kazuhiro Takagi, Akio Iwasaki, Ichiro Kamei, Koji Satsuma, Yuichi Yoshioka, Naoki Harada

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   75 ( 13 )   4452 - 4458   2009.7

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    DOI： 10.1128/AEM.02329-08

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  • Different substrate specificities of two triazine hydrolases (TrzNs) from Nocardioides species Reviewed

    Kenichi Yamazaki, Kunihiko Fujii, Akio Iwasaki, Kazuhiro Takagi, Koji Satsuma, Naoki Harada, Tai Uchimura

    FEMS MICROBIOLOGY LETTERS   286 ( 2 )   171 - 177   2008.9

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    DOI： 10.1111/j.1574-6968.2008.01271.x

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  • Simultaneous biodegradation of chloro and methylthio-s-triazines using charcoal enriched with a newly developed bacterial consortium Reviewed

    Ken-ichi Yamazaki, Kazuhiro Takagi, Kunihiko Fujii, Akio Iwasaki, Naoki Harada, Tai Uchimura

    JOURNAL OF PESTICIDE SCIENCE   33 ( 3 )   266 - 270   2008

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    DOI： 10.1584/jpestics.G08-08

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  • Enhanced methanogenesis in the vicinity of rice straw residues in surface layers of a paddy soil Reviewed

    Harada N, Nishiyama M, Otsuka S, Matsumoto S

    土と微生物   61 ( 1 )   17 - 21   2007.4

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    Methanogenesis in irrigated rice fields is carried out by methanogenic archaea under strictly anaerobic conditions. In the present study, the effect of rice straw on methanogenesis in surface layers of paddy soil was investigated in a pot experiment with surface application of rice straw. Rice straw application led to a significant development of reduced conditions in the surface soil, and markedly increased methane (CH_4) emissions. Potential methanogenic activity in the straw residues and the soil layer immediately under the residues (0〜1 cm depth) was considerably promoted, while that in the deeper soil layers (4〜6 cm depth) was not affected by the residues of rice straw. These results indicate that the increased CH_4 emission in the treatment with surface application of rice straw was due to the activation of methanogenesis in the vicinity of the residues. Consequently, in the present study, it was demonstrated that rice straw application enhanced methanogenesis even in the surface layers of paddy soil, which are generally considered to be oxic layers.

    DOI： 10.18946/jssm.61.1_17

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    Other Link： http://hdl.handle.net/10191/24984

  • Isolation and characterization of a novel simazine-degrading beta-proteobacterium and detection of genes encoding s-triazine-degrading enzymes Reviewed

    Akio Iwasaki, Kazuhiro Takagi, Yuichi Yoshioka, Kunihiko Fujii, Yuki Kojima, Naoki Harada

    PEST MANAGEMENT SCIENCE   63 ( 3 )   261 - 268   2007.3

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    DOI： 10.1002/ps.1334

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  • Biodegradation of methylthio-s-triazinessd where science by Rhodococcus sp strain FJ1117YT, and production of the corresponding methylsulfinyl, methylsulfonyl and hydroxy analogues Reviewed

    Kunihiko Fujii, Kazuhiro Takagi, Syuntaro Hiradate, Akio Iwasaki, Naoki Harada

    PEST MANAGEMENT SCIENCE   63 ( 3 )   254 - 260   2007.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/ps.1331

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  • Adsorptive characteristics of phosphorylated cellulose material against cadmium Reviewed

    Harada N, Suda M, Saito Y, Yabusaki K

    Japanese Journal of Soil Science and Plant Nutrition   78 ( 1 )   89 - 91   2007.2

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    DOI： 10.20710/dojo.78.1_89

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    Other Link： http://agriknowledge.affrc.go.jp/RN/2010734296

  • Transformation of methylthio-s-triazines via sulfur oxidation by strain JUN7, a Bacillus cereus species Reviewed

    Naoki Harada, Kazuhiro Takagi, Kunihiko Fujii, Akio Iwasaki

    SOIL BIOLOGY & BIOCHEMISTRY   38 ( 9 )   2952 - 2957   2006.9

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    DOI： 10.1016/j.soilbio.2006.03.018

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  • Isolation and characterization of microorganisms capable of hydrolysing the herbicide mefenacet Reviewed

    N Harada, K Takagi, A Harazono, K Fujii, A Iwasaki

    SOIL BIOLOGY & BIOCHEMISTRY   38 ( 1 )   173 - 179   2006.1

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    DOI： 10.1016/j.soilbio.2005.04.015

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  • Effects of inoculation of phototrophic purple bacteria on grain yield of rice and nitrogenase activity of paddy soil in a pot experiment Reviewed

    N Harada, M Nishiyama, S Otsuka, S Matsumoto

    SOIL SCIENCE AND PLANT NUTRITION   51 ( 3 )   361 - 367   2005.6

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    DOI： 10.1111/j.1747-0765.2005.tb00041.x

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  • Influences of indigenous phototrophs on methane emissions from a straw-amended paddy soil Reviewed

    N Harada, S Otsuka, M Nishiyama, S Matsumoto

    BIOLOGY AND FERTILITY OF SOILS   41 ( 1 )   46 - 51   2005.1

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    DOI： 10.1007/s00374-004-0793-8

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  • Characteristics of phototrophic purple bacteria isolated from a Japanese paddy soil Reviewed

    N Harada, S Otsuka, M Nishiyama, S Matsumoto

    SOIL SCIENCE AND PLANT NUTRITION   49 ( 4 )   521 - 526   2003.8

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    DOI： 10.1080/00380768.2003.10410041

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  • Phototrophic N-2 fixation suppressed by activated sulfate reduction in anoxic rice soil slurries Reviewed

    N Harada, M Nishiyama, S Matsumoto

    CURRENT MICROBIOLOGY   42 ( 6 )   393 - 397   2001.6

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    DOI： 10.1007/s002840010236

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  • Inhibition of methanogens increases photo-dependent nitrogenase activities in anoxic paddy soil amended with rice straw Reviewed

    N Harada, M Nishiyama, S Matsumoto

    FEMS MICROBIOLOGY ECOLOGY   35 ( 3 )   231 - 238   2001.5

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    DOI： 10.1016/S0168-6496(01)00095-2

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Books

  • 土壌微生物学

    原田 直樹（ Role： Joint author ,  第13章 微生物による環境汚染物質などの分解）

    2018.8 

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  • 農と土のある暮らしを次世代へ－原発事故からの農村の再生

    原田 直樹（ Role： Joint author ,  第1部第2章 農地の放射性セシウム汚染と作物への影響）

    コモンズ  2018.7 

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  • BISHAMONの軌跡-II －福島支援5年間の記録

    原田 直樹（ Role： Joint author ,  第4章3. 大豆の放射性セシウム吸収抑制に向けた取組み 及び 第4章4. 地域特産物に目を向けて～桑葉の放射性セシウム対策～）

    2016.11 

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MISC

  • もっと菌根の世界 知られざる根圏のパートナーシップ 齋藤雅典（編著） 四六判，カラー口絵8頁+344頁，2,700円（税別） 築地書館，2023年9月22日発行

    原田 直樹

    日本土壌肥料学雑誌   95 ( 1 )   75 - 75   2024.2

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    Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

    DOI： 10.20710/dojo.95.1_75

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  • 放射線災害後の福島の農業－数値が語るこの10年－ Invited

    原田直樹

    耕   150   35 - 40   2021.3

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  • Subcutaneous injection of interferon gamma therapy could be useful for anti–IFN-γ autoantibody associated disseminated nontuberculous mycobacterial infection

    Masanori Harada, Kazuki Furuhashi, Masato Karayama, Yuzo Suzuki, Hironao Hozumi, Noriyuki Enomoto, Tomoyuki Fujisawa, Yutaro Nakamura, Naoki Inui, Takafumi Suda

    Journal of Infection and Chemotherapy   27 ( 2 )   373 - 378   2021.2

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  • 3-1-22 Effects of biochar amendment on community structure of protists and their predatory effects on bacterial community composition in a rice rhizosphere（3-1 土壌生物の生態と機能 2020年度岡山大会）

    日本土壌肥料学会講演要旨集   66   28 - 28   2020.9

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    Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

    DOI： 10.20710/dohikouen.66.0_28_1

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  • 10 Effect of Biochar Amendment on Heterotrophic Protists in a Rice（<i>Oryza sativa</i> L. Nipponbare）Rhizosphere（関東支部講演会）

    日本土壌肥料学会講演要旨集   65   248 - 248   2019.9

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    DOI： 10.20710/dohikouen.65.0_248_1

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  • 住民と大学・研究機関との連携による放射線計測と試験栽培－福島県飯舘村大久保外内地区－

    石井秀樹, 服部正幸, 棚橋知春, 小松知未, 後藤淳, 内藤航, 上坂元紀, 原田直樹, 野中昌法, 守友裕一

    福島大学地域創造   29   46 - 56   2017.9

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  • ロシア沿海地方における大豆栽培

    大山卓爾, 原田直樹, 小野祐樹, 長谷川英雄

    新潟アグロノミー   51 ( 28 )   33   2015.12

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  • 里山－谷津田から河川に放出される放射性 セシウムの実態解明

    原田直樹, 野中昌法, 吉川夏樹

    河川整備基金助成事業成果報告書   24–1211–013   1 - 23   2013.5

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  • Fundamental studies on behavior of arsenicals in rice soil

    385 - 390   2010.8

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  • 水田土壌における光合成細菌の存在と意義

    原田直樹

    新潟アグロノミー   ( 44 )   47 - 56   2008.12

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  • Biodegradation of s-triazine herbicides by soil microorganisms and their application for in situ bioremediation

    Harada N

    新潟大学農学部研究報告   60 ( 1 )   1 - 7   2007.8

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)   Publisher：新潟大学農学部  

    Pseudomonas sp. strain ADP has been studied most extensively. Dechlorination of the s-triazine ring by the strain is mediated by atrazine chlorohydrolase (AtzA) and the subsequent degradation is associated with AtzBCDEF. A novel simazine-degrading β-proteobacterium strain CDB21 isolated by the author's group also possessed entire-set of genes of these enzymes (atzABCDEF). However, while atzA is located between ORF30 and atzB in strain ADP, ORF30 fused with atzB in strain CDB21. This indicates that localization of atzA in CDB21 differs from that in the pseudomonad, meaning that genetic variations regarding the degradation genes exist among microorganisms capable of degrading chloro-s-triazines. It is known that methylthio-s-triazines can be transformed to the corresponding hydroxy analogues via sulfur oxidation in aerobic and flooded soil. Recently, two types of bacteria that engage the process were isolated. One is Bacillus cereus strain JUN7 that can degrade methylthio-s-triazines to give the methylsulfinyl and hydroxy analogues under nutrient-rich conditions such as those with the Luria-Bertani medium. The other is Rhodococcus sp. strain FJ1117YT that is able to degrade methylthios-triazines supplied as the sole sulfur source. These strains were unable to degrade chloro-s-triazines. No gene encoding AtzA or TrzN (triazine hydrolase) was found in these strains, suggesting that they may have another metabolic system. Using a special charcoal material enriched with a simazine-degrading bacterial consortium involving strain CDB21, in situ bioremediation study was conducted in a golf course, where simazine is routinely applied for preservation of turf. As a result, the material was effective for preventing penetration of simazine into subsoils and aquatic environments nearby for approximately 2 years. This result suggests that use of adequate habitat materials is preferable to maintain population and activity of inoculant bacteria applied to soil environment.

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    Other Link： http://agriknowledge.affrc.go.jp/RN/2010742733

  • In situ bioremediation of pesticide-contaminated soil by using charcoal enriched with consortia of degrading bacteria

    Takagi K, Yoshioka Y, Iwasaki A, Harada N

    Journal of Resources and Environment   41 ( 6 )   79 - 85   2005.5

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Presentations

  • P3-1-3 有機及び慣行水田における土壌微生物群集構造の比較－長野および宮城におけるケーススタディ－（ポスター，3-1 土壌生物の生態と機能，2018年度神奈川大会）

    鈴木 一輝, 今野 凛, 米山 菜穂子, 三木 孝昭, 徐 啓聡, 大久保 慎二, 岩石 真嗣, 原田 直樹

    日本土壌肥料学会講演要旨集  2018  一般社団法人 日本土壌肥料学会

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  • 8-1-13 水田の水口付近における土壌及びイネの放射性セシウム濃度の分布（8-1 環境保全，2016年度佐賀大会）

    鈴木 啓真, 中島 浩世, 鶴田 綾介, 吉川 夏樹, 石井 秀樹, 野川 憲夫, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2016  一般社団法人 日本土壌肥料学会

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    Event date： 2016

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  • 8-1-28 改植による桑の葉の<sup>137</sup>Cs 濃度の低減（8-1 環境保全，2016年度佐賀大会）

    木幡 裕介, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2016  一般社団法人 日本土壌肥料学会

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  • P8-1-9 化学肥料(硫酸カリ)と有機質肥料によるダイズの放射性セシウム吸収抑制効果の比較(ポスター,8-1 環境保全,2015年度京都大会)

    本島 彩香, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2015.9  一般社団法人日本土壌肥料学会

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  • 33 太田川流域水田における放射性セシウムの挙動と水稲への吸収(関東支部講演会,2014年度各支部会)

    荘司 亮介, 片桐 優亮, 小笠 真理恵, 田巻 翔平, 奥村 健郎, 石井 秀樹, 野川 憲夫, 吉川 夏樹, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2015.9  一般社団法人日本土壌肥料学会

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  • P8-1-4 農業用水がイネの放射性セシウム濃度に与える影響 : 流下試験による検討(ポスター,8-1 環境保全,2015年度京都大会)

    鈴木 啓真, 荘司 亮介, 弦巻 貴大, 吉澤 涼太, 田巻 翔平, 吉川 夏樹, 石井 秀樹, 野川 憲夫, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2015.9  一般社団法人日本土壌肥料学会

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  • P8-1-16 桑園における放射性Csの吸収特性と吸収抑制法の検討(ポスター,8-1 環境保全,2015年度京都大会)

    木幡 裕介, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2015.9  一般社団法人日本土壌肥料学会

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  • P8-1-5 水稲栽培期間における水田土壌表層への放射性セシウムの蓄積(ポスター,8-1 環境保全,2015年度京都大会)

    荘司 亮介, 鈴木 啓真, 弦巻 貴大, 田巻 翔平, 吉川 夏樹, 石井 秀樹, 野川 憲夫, 原田 直樹, 野中 昌大

    日本土壌肥料学会講演要旨集  2015.9  一般社団法人日本土壌肥料学会

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  • 地中海沿岸地域における長期有機連用がトウモロコシ根の糸状菌叢に与える影響(2015年度大会講演要旨)

    鈴木 一輝, 戸井田 千佳, Turgay Oguz Can, Ortas Ibrahim, 原田 直樹, 野中 昌法

    土と微生物  2015  日本土壌微生物学会

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  • 次世代シーケンサーを用いた土壌細菌群集構造解析による有機水田土壌のプロファイリングの試み(2015年度大会講演要旨)

    竹村 真奈美, 鈴木 一輝, 野中 昌法, 原田 直樹

    土と微生物  2015  日本土壌微生物学会

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  • 8-1-10 カリ施肥によるダイズの放射性Cs吸収抑制(8-1 環境保全,2014年度東京大会)

    本島 彩香, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2014.9  一般社団法人日本土壌肥料学会

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  • P3-1-16 深層施肥された窒素のアーバスキュラー菌根菌を介した植物への吸収(ポスター,3-1 土壌生物の生態と機能,2014年度東京大会)

    柏 直也, 岩佐 悦道, 佐藤 孝, 大山 卓爾, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2014.9  一般社団法人日本土壌肥料学会

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  • 8-1-19 中山間地水田における放射性セシウムの挙動 : 福島第一原発事故後3年間の経年変化(8-1 環境保全,2014年度東京大会)

    原田 直樹, 宮本 昇平, 吉澤 涼太, 小原 ひとみ, 小笠 真理恵, 宮津 進, 吉川 夏樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2014.9  一般社団法人日本土壌肥料学会

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  • 中国黒竜江省に分布するアルカリ塩類土壌におけるアーバスキュラー菌根菌の多様性(2013年度大会一般講演要旨)

    成沢 大志, 原田 直樹, 野中 昌法, 司 振江, 黄 彦

    土と微生物  2014.4  日本土壌微生物学会

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  • 7-1-11 窒素深層施肥による水田フル活用 : (7)転換畑における亜酸化窒素の発生抑制効果(7-1 肥料および施肥法,2013年度名古屋大会)

    池澤 弘, 坂入 恵美子, 吉本 悠太郎, 南雲 芳文, 服部 誠, 藤田 与一, 野中 昌法, 原田 直樹

    日本土壌肥料学会講演要旨集  2013.9  一般社団法人日本土壌肥料学会

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  • P8-1-20 土壌特性がマメ科作物の放射性セシウム吸収に与える影響(ポスター,8-1 環境保全,2013年度名古屋大会)

    本島 彩香, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2013.9  一般社団法人日本土壌肥料学会

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  • 50 有機稲作が水田土壌の細菌群集構造に与える影響の解析(関東支部講演会,2012年度各支部会)

    工藤 航, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2013.9  一般社団法人日本土壌肥料学会

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  • 37 石灰窒素深層施肥によるダイズ畑からの亜酸化窒素発生抑制効果の検証(関東支部講演会,2012年度各支部会)

    池澤 弘, 原田 直樹, 南雲 芳文, 服部 誠, 野中 昌法

    日本土壌肥料学会講演要旨集  2013.9  一般社団法人日本土壌肥料学会

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  • P8-1-24 農業用水からの谷地田への放射性セシウム流入と水稲への吸収(ポスター,8-1 環境保全,2013年度名古屋大会)

    吉澤 涼太, 宮本 昇平, 小原 ひとみ, 小笠 真理恵, 宮津 進, 吉川 夏樹, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2013.9  一般社団法人日本土壌肥料学会

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  • P3-1-15 トルコ黒海沿岸酸性土壌における施肥管理とチャノキ共生アーバスキュラー菌根菌の多様性(ポスター,3-1 土壌生物の生態と機能,2013年度名古屋大会)

    鈴木 一輝, Turgay Oguz Can, Akca Muhittin Onur, Ali Ergul, Ozer Pmar, 原田 直樹, 野中 昌法

    日土肥講要  2013  一般社団法人 日本土壌肥料学会

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  • P22-10 還元的土壌中におけるジフェニルアルシン酸の変換生成物の同定(S22.放射性セシウムの永年性作物への移行,22.環境保全,2012年度鳥取大会)

    久富 志穂子, Guan Ling, 藤井 邦彦, 中島 真美, 野中 昌法, 原田 直樹

    日本土壌肥料学会講演要旨集  2012.9  一般社団法人日本土壌肥料学会

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  • P8-2 母材および管理の異なるトルコ耕地土壌におけるアーバスキュラー菌根菌の多様性(8.共生,2012年度鳥取大会)

    鈴木 一輝, Turgay Oguz Can, 原田 直樹, 野中 昌法

    日土肥講要  2012  一般社団法人 日本土壌肥料学会

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  • トルコ地中海地域アダナの長期有機連用圃場におけるアーバスキュラー菌根菌の多様性(2012年度土壌微生物学会講演要旨)

    武藤 拓也, 鈴木 一輝, 成沢 大志, 海津 朋之, 原田 直樹, 野中 昌法, Turgay Oguz Can, Ortas Ibrahim

    土と微生物  2012  日本土壌微生物学会

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  • 6-19 有機稲作における窒素固定菌群の解析(第2報) : イネ根から抽出したRNAを用いたnifHの多様性解析(6.土壌生物)

    原田 直樹, 菊地 靖子, 野中 昌法

    日本土壌肥料学会講演要旨集  2011.8  一般社団法人日本土壌肥料学会

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  • 新潟県の海岸砂丘地植物と共生するアーバスキュラー菌根菌(2010年度大会一般講演要旨)

    海津 朋之, 武藤 拓也, 原田 直樹, 野中 昌法

    土と微生物  2010  日本土壌微生物学会

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  • 食品残渣の土壌処理に伴う微生物群集構造変化(2010年度大会一般講演要旨)

    飯尾 真也, 原田 直樹, 野中 昌法

    土と微生物  2010  日本土壌微生物学会

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  • Does inoculation of anoxygenic photosynthetic bacteria contribute to rice production?

    Naoki Harada

    Development of Integrated Pest Management in Asia and Africa, Science and Technics Publishing House  2009.12 

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  • Bacterial degradation of persistent agrochmicals and application of charcoal as a tool for in situ bioremediation

    Naoki Harada

    Development of Integrated Pest Management in Asia and Africa, Science and Technics Publishing House  2009.12 

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  • 6-32 冬期湛水水田における土壌及び稲わらの細菌群集構造の解析(6.土壌生物,2009年度京都大会)

    原田 直樹, 高山 大輝, 野中 昌法

    日本土壌肥料学会講演要旨集  2009.9  一般社団法人日本土壌肥料学会

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  • 16 食品残渣投入後の土壌化学性及び微生物性の変化(関東支部講演会,2008年度各支部会)

    飯尾 真也, 渡辺 聡, 宮内 直美, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2009.9  一般社団法人日本土壌肥料学会

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  • P6-4 有機稲作における窒素固定菌群の解析(第1報) : イネ根におけるnifHの多様性(ポスター紹介,6.土壌生物,2009年度京都大会)

    菊地 靖子, 山家 悠平, 飯尾 真也, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2009.9  一般社団法人日本土壌肥料学会

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  • 17 新潟県の海岸砂丘植物のアーバスキュラー菌根菌の解析(関東支部講演会,2008年度各支部会)

    海津 朋之, 坂田 益朗, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2009.9  一般社団法人日本土壌肥料学会

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  • P21-2 アーバスキュラー菌根菌と森林土壌埋土種子由来の植物を用いた自然環境の修復(ポスター紹介,21.緑化技術,2009年度京都大会)

    武藤 拓也, 原田 直樹, 野中 昌法

    日本土壌肥料学会講演要旨集  2009.9  一般社団法人日本土壌肥料学会

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  • Substrate specificities of two strains of Nocardioides for degradation of s-triazine herbicides : 1. Relationship between nucleotide sequences of triazine hydrolase gene (trzN) and substrate specificities

    YAMAZAKI Kenichi, TAKAGI Kazuhiro, FUJII Kunihiko, IWASAKI Akio, SATSUMA Koji, HARADA Naoki, UCHIMURA Tai

    2008.3  Pesticide Science Society of Japan

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  • Simultaneous biodegradation of chloro and methylthio s-triazines by a newly developed bacterial consortium (2)

    YAMAZAKI Kenichi, TAKAGI Kazuhiro, HARADA Naoki, FUJII Kunihiko, UCHIMURA Tai

    2007.3  Pesticide Science Society of Japan

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  • Simultaneous biodegradation of chloro and methylthio s-triazines by a newly developed bacterial consortium

    YAMAZAKI Kenichi, TAKAGI Kazuhiro, HARADA Naoki, FUJII Kunihiko, UCHIMURA Tai

    2006.3  Pesticide Science Society of Japan

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  • 6-23 シメトリン分解菌の単離とその性質(6.土壌生物,日本土壌肥料学会 2005年度大会講演要旨集)

    原田 直樹, 高木 和広, 藤井 邦彦, 岩崎 昭夫

    日本土壌肥料学会講演要旨集  2005.9  一般社団法人日本土壌肥料学会

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  • Characteristics of mefenacet degrading bacteria isolated from Japanese paddy soil and bark compost

    HARADA Naoki, TAKAGI Kazuhiro, HARAZONO Aiko, IWASAKI Akio

    2004.3  Pesticide Science Society of Japan

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  • 6-4 光合成細菌の接種が水田土壌からのメタン発生に与える影響(6.土壌生物)

    原田 直樹, 西山 雅也, 大塚 重人, 松本 聰

    日本土壌肥料学会講演要旨集  2002.3  一般社団法人日本土壌肥料学会

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  • 6-19 稲わら施用湛水土壌における光合成細菌に関する研究(第2報) : 光合成細菌の単離とその性質(6.土壌生物)

    原田 直樹, 西山 雅也, 松本 聰

    日本土壌肥料学会講演要旨集  2000.3  一般社団法人日本土壌肥料学会

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  • 6-19 稲わら施用湛水土壌における光合成細菌に関する研究(第1報) : メタン生成阻害下でのアセチレン還元活性(ARA)の増大(6.土壌生物)

    原田 直樹, 西山 雅也, 松本 聰

    日本土壌肥料学会講演要旨集  1999.7  一般社団法人日本土壌肥料学会

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  • Arbuscular Mycorrhizal Fungal diversity in saline-alkaline soils of Turkey International conference

    Tomoyuki KAIDZU, Oguz Can TURGAY, Naoki HARADA, Masanori NONAKA

    The 7th International Conference on Mycorrhiza  2013.1 

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  • Weed control in organic rice farming: effect of surface application of organic materials International conference

    Yamaga Y, Harada N, Nonaka M

    The 5th international meeting for the development of integrated pest management (IPM) in Asia and Africa  2012.12 

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  • 原発事故から1年10ヵ月、ゆうきの里東和ふるさとづくり協議会との協働の復興・研究活動を振り返る・問われる科学者の姿勢！

    野中昌法, 原田直樹, 小松崎将一, 金子信博, 木村園子ドロテア

    第13回日本有機農業学会大会  2012.12 

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  • 有機稲作が水田土壌の細菌群集構造に与える影響の解析

    工藤航, 野中昌法, 原田直樹

    2012年度日本土壌肥料学会関東支部大会  2012.12 

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  • 石灰窒素深層施肥によるダイズ畑からの亜酸化窒素発生抑制効果の検証

    池澤弘, 野中昌法, 原田直樹

    2012年度日本土壌肥料学会関東支部大会  2012.12 

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  • Availability of sulfate and electron donating substrates influences transformation of diphenylarsinic acid in anaerobic soil International conference

    Ling GUAN, Shihoko HISATOMI, Kunihiko FUJII, Masanori NONAKA, Naoki HARADA

    SoilRem2012 & SiteRem2012  2012.9 

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  • Biotransformation of diphenylarsinic acid under anaerobic soil conditions International conference

    Naoki HARADA, Ling GUAN, Shihoko HISATOMI, Mami NAKAJIMA, Kunihiko FUJII, Masanori NONAKA

    SoilRem2012 & SiteRem2012  2012.9 

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  • Determination of a sulfur containing arsenic metabolite in the anaerobic soil culture contaminated with diphenylarsinic acid International conference

    Shihoko HISATOMI, Ling GUAN, Kunihiko FUJII, Mami NAKAJIMA, Masanori NONAKA, Naoki HARADA

    SoilRem2012 & SiteRem2012  2012.9 

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  • 母材および管理の異なるトルコ耕地土壌中の土着アーバスキュラー菌根菌の多様性

    鈴木一輝, 原田直樹, 野中昌法, Oguz Can Turgay

    2012年度日本土壌肥料学会島根大会  2012.9 

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  • 還元的土壌中におけるジフェニルアルシン酸の変換生成物の同定

    久富志穂子, Guan Ling, 藤井邦彦, 中島真美, 野中昌法, 原田直樹

    2012年度日本土壌肥料学会島根大会  2012.9 

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  • Screening of dieldrin-degrading fungi from dieldrin-residual and healthy upland soils International conference

    Emiko SAKAIRI, Masanori NONAKA, Naoki HARADA

    The 4th International Symposium on “Modern Agriculture and Rural Economic Development in Asia”  2012.7 

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  • Diversity of indigenous arbuscular mycorrizal fungi in saline-alkaline soil on Heilongjiang, China International conference

    Taishi NARISAWA, Naoki HARADA, Masanori NONAKA, Zhenjiang SI, Yan HUANG

    The 4th International Symposium on “Modern Agriculture and Rural Economic Development in Asia”  2012.7 

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  • 福島第一原発事故後の新潟県内水田における土壌中放射性セシウムの垂直及び水平分布

    原田直樹, 野中昌法

    第49回 アイソトープ・放射線 研究発表会  2012.7 

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  • 福島第一原発事故による里山－農地生態系の放射性セシウム汚染 ～福島県二本松市における水田土壌及び水稲の放射能濃度～

    宮本昇平, 原田直樹, 野中昌法

    第49回 アイソトープ・放射線 研究発表会  2012.7 

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  • トルコ地中海地域アダナの長期有機連用圃場におけるアーバスキュラー菌根菌の多様性

    武藤拓也, 鈴木一輝, 成沢大志, 海津朋之, 原田直樹, 野中昌法, Oguz Can Turgay, Ibrahim Ortas

    日本土壌微生物学会2012年度大会  2012.6 

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  • アーバスキュラー菌根菌に及ぼす高pHの影響

    成沢大志, 小林由季, 原田直樹, 野中昌法

    日本土壌微生物学会2012年度大会  2012.6 

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  • Arbuscular Mycorrhizal fungal diversity under long-term inorganic and organic fertilization practices in Mediterranean Turkey International conference

    Tomoyuki KAIDZU, Oguz Can TURGAY, Kazuki SUZUKI, Taishi NARISAWA, Takuya BUTO, Naoki HARADA, Masanori NONAKA, Ibrahim ORTAS

    8th International Soil Science Congress, “Land Degradation and Challenges in Soil Management”  2012.5 

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  • 福島および茨城における耕起・不耕起による放射能汚染の土層分布の変化

    小松崎将一, 野中昌法, 原田直樹, 東達哉

    日本農作業学会第47回講演会  2012.3 

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  • 二本松東和地域の里山・水田の放射能汚染の実態と取り組み

    野中昌法, 原田直樹, 小松崎将一

    第12回日本有機農業学会大会  2011.12 

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  • Degradation of diphenylarsinic acid in anaerobic paddy soil and its effects on soil microbial communities International conference

    Ling Guan, Yasushi Ono, Takuya Takahashi, Naoki Harada, Kunihiko Fujii, Xiaolan Liu, Masanori Nonaka

    International Symposium on Environmental Biogeochemistry XX  2011.9 

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  • Expression of nifH genes in roots of rice, Oryza sativa L., under conventional and organic managements International conference

    Naoki Harada, Yasuko Kikuchi, Masanori Nonaka

    International Symposium on Environmental Biogeochemistry XX  2011.9 

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  • Arbuscular mycorrhizal fungi colonizing with coastal plants in Niigata, Japan International conference

    Tomoyuki KAIDZU, Masuro SAKATA, Takuya BUTO, Naoki HARADA, Masanori NONAKA

    International Symposium on Environmental Biogeochemistry XX  2011.9 

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  • 有機稲作における窒素固定菌群の解析（第2報） イネ根から抽出したRNAを用いたnifHの多様性解析

    原田直樹, 菊地靖子, 野中昌法

    日本土壌肥料学会大会  2011.8 

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  • Analysis of soil microbes engaging in anaerobic transformation of diphenylarsinic acid

    Ling GUAN, Naoki HARADA, Masanori NONAKA

    第16回ヒ素シンポジウム  2011.2 

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  • Effects of intentional winter fallow flooding on bacterial community structures of rice soil in Niigata Plain, Japan International conference

    Naoki HARADA, Daiki TAKAYAMA, Masanori NONAKA

    The 4th international meeting for the development of integrated pest management (IPM) in Asia and Africa  2011.1 

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  • Impacts of diphenylarsinic acid on microbial diversities in paddy soil amended with rice straw International conference

    Ling GUAN, Naoki HARADA, Masanori NONAKA

    The 4th international meeting for the development of integrated pest management (IPM) in Asia and Africa  2011.1 

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  • Inoculation effects of arbuscular mycorrhizal fungi on regeneration of shallow landslide scar with seed bank soil in Niigata-Japan forest area International conference

    Takuya BUTO, Naoki HARADA, Masanori NONAKA

    1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation  2010.9 

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  • 食品残渣の土壌処理に伴う微生物群集構造変化

    飯尾真也, 原田直樹, 野中昌法

    日本土壌微生物学会  2010.6 

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  • 新潟県の海岸砂丘地植物と共生するアーバスキュラー菌根菌

    海津朋之, 武藤拓也, 原田直樹, 野中昌法

    日本土壌微生物学会  2010.6 

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  • Diversities of diazotrophic communities in rice soil revealed by molecular-based ecological approaches International conference

    Naoki HARADA, Yasuko KIKUCHI, Masanori NONAKA

    The 3th international meeting for the development of integrated pest management (IPM) in Asia and Africa  2009.12 

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  • Regeneration of shallow landslide slope by seed bank soil and indigenous arbuscular mycorrhizal(AM) fungi in Niigata forest area International conference

    Takuya BUTO, Naoki HARADA, Masanori NONAKA

    The 3rd International Symposium on Strategies for Sustainability in Food Production, Agriculture and the Environment in Asia  2009.9 

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  • Changes in chemical properties and bacterial communities in soil amended with food waste International conference

    Shinya IIO, Naoki HARADA, Masanori NONAKA

    The 3rd International Symposium on Strategies for Sustainability in Food Production, Agriculture and the Environment in Asia  2009.9 

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  • Comparative nitrogen dynamics in organic and conventional tomato cultivation in Niigata prefecture International conference

    Yui FUJINO, Naoki HARADA, Masanori NONAKA

    The 3rd International Symposium on Strategies for Sustainability in Food Production, Agriculture and the Environment in Asia  2009.9 

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  • Arbuscular mycorrhizal fungi (AMF) colonizing with coastal pants in Niigata International conference

    Tomoyuki KAIDZU, Naoki HARADA, Masanori NONAKA

    The 3rd International Symposium on Strategies for Sustainability in Food Production, Agriculture and the Environment in Asia  2009.9 

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  • アーバスキュラー菌根菌と森林土壌埋土種子由来の植物を用いた自然環境の修復

    武藤拓也, 原田直樹, 野中昌法

    日本土壌肥料学会大会  2009.9 

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  • 冬期湛水水田における土壌及び稲わらの細菌群集構造の解析

    原田直樹, 高山大輝, 野中昌法

    日本土壌肥料学会大会  2009.9 

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  • 有機稲作における窒素固定菌群の解析（第１報） イネ根におけるnifHの多様性

    菊地靖子, 山家悠平, 飯尾真也, 原田直樹, 野中昌法

    日本土壌肥料学会大会  2009.9 

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  • Bacterial degradation of persistent agrochemicals and application of charcoal as a tool for in situ bioremediation International conference

    Naoki HARADA

    The 2nd international meeting for the development of integrated pest management (IPM) in Asia and Africa  2008.12 

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  • Does inoculation of anoxygenic photosynthetic bacteria contribute to rice production? International conference

    Naoki HARADA

    The 2nd international meeting for the development of integrated pest management (IPM) in Asia and Africa  2008.12 

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  • 食品残渣投入後の土壌化学性及び微生物性の変化

    飯尾真也, 渡辺 聡, 宮内直美, 原田直樹, 野中昌法

    日本土壌肥料学会関東支部大会  2008.11 

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  • 新潟県の海岸砂丘植物のアーバスキュラー菌根菌の解析

    海津朋之, 坂田益朗, 原田直樹, 野中昌法

    日本土壌肥料学会関東支部大会  2008.11 

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  • Relationship between nucleotide sequences of triazine hydrolase genes and substrate specificities of s-triazines from two Nocardioides species International conference

    Kenichi Yamazaki, Kazuhiro Takagi, Akio Iwasaki, Kunihiko Fujii, Koji Satsuma, Naoki Harada, Tai Uchimura

    1st International Symposium on Water in Agriculture and Forestry: Challenges, Technological Solutions and Innovations  2008.10 

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  • Simultaneous biodegradation of chloro- and methylthio-s-triazines using charcoal enriched with a newly developed bacterial consortium International conference

    Kazuhiro Takagi, Kenichi Yamazaki, Kunihiko Fujii, Akio Iwasaki, Naoki Harada

    The 3rd International Conference on Soil Pollution and Remediation  2008.10 

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  • Different substrate specificities between two strains of Nocardioides in degradation of s-triazines International conference

    Kenichi Yamazaki, Kazuhiro Takagi, Akio Iwasaki, Kunihiko Fujii, Koji Satsuma, Naoki Harada, Tai Uchimura

    The 3rd International Conference on Soil Pollution and Remediation  2008.10 

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  • Nocardioides属細菌2菌株のトリアジン系除草剤に対する基質分解特異性 第１報 Triazine hydrolase 遺伝子(trzN)の塩基配列と基質特異性の関連性

    山崎健一, 高木和広, 藤井邦彦, 岩崎昭夫, 薩摩孝次, 原田直樹, 内村泰

    日本農薬学会第33回大会  2008.3 

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  • Nocardioides属細菌2菌株のトリアジン系除草剤に対する基質分解特異性 第２報 大腸菌で発現された野生型及びキメラtrzNの基質特異性

    藤井邦彦, 高木和広, 薩摩孝次, 山崎健一, 原田直樹, 岩崎昭夫

    日本農薬学会第33回大会  2008.3 

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  • Metabolic pathways of hexachlorobenzene (HCB), quintozene (PCNB) and pentachlorophenol (PCP) by a newly isolated strain Nocardiodes sp. PD653 under aerobic conditions International conference

    Kazuhiro TAKAGI, Yuichi YOSHIOKA, Akio IWASAKI, Ichiro KAMEI, Naoki HARADA

    Dioxin 2007  2007.9 

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  • ジフェニルアルシン酸分解菌Shinorhizobium sp. L2406株による有機ヒ素化合物の無機化

    原田直樹, 高木和広, 馬場浩司

    日本土壌肥料学会大会  2007.8 

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  • 水田土壌からのジフェニルアルシン酸分解菌単離の試み

    原田直樹, 高木和広

    日本土壌肥料学会大会  2006.9 

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  • Aerobic mineralization of hexachlorobenzene by Nocardioides sp. PD653 International conference

    Takagi K, Iwasaki A, Satsuma K, Masuda M, Harada N

    The 11th IUPAC International Congress of Pesticide Chemistry  2006.8 

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  • Bacterial degradation of simetryn, a moderately persistent methylthio-substituted s-triazine herbicide International conference

    Naoki HARADA, Kazuhiro TAKAGI, Kunihiko FUJII, Akio IWASAKI

    International Chemical Congress of Pacific Basin Societies  2005.12 

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  • シメトリン分解菌の単離とその性質

    原田直樹, 高木和広, 藤井邦彦, 岩崎昭夫

    日本土壌肥料学会大会  2005.9 

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  • Monitoring of a simazine-degrading bacterium during in situ bioremediation study by the use of specific DNA probes International conference

    wasaki A, Takagi K, Yoshioka Y, Kojima Y, Harada N

    The 2nd International Conference on Soil Pollution and Remediation  2004.11 

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  • リン酸キレート繊維によるカドミウム汚染水田土壌の浄化1：－リン酸キレート繊維の特徴－

    原田直樹, 須田誠, 斎藤陽子, 薮崎克己

    日本土壌肥料学会大会  2004.9 

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  • リン酸キレート繊維によるカドミウム汚染水田土壌の浄化2：－カドミウム除去能の評価－

    原田直樹, 須田誠, 斎藤陽子, 薮崎克己

    日本土壌肥料学会大会  2004.9 

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  • リン酸キレート繊維による重金属汚染土壌の浄化に関する基礎検討

    原田直樹, 須田誠, 斎藤陽子, 薮崎克己

    第10回地下水・土壌汚染とその防止対策に関する研究集会  2004.7 

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  • 土壌等から単離したmefenacet分解菌の特徴

    原田直樹, 高木和広, 原園愛子, 岩崎昭夫

    日本農薬学会第29回大会  2004.3 

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  • Isolation and characterization of novel simazine-degrading bacteria International conference

    Akio IWASAKI, Kazuhiro TAKAGI, Yuichi YOSHIOKA, Naoki HARADA

    The Third International Conference on Contaminants in the Soil Environment in the Australasia-Pacific Region  2003.11 

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  • 光合成細菌の接種が水田土壌からのメタン発生に与える影響

    原田直樹, 西山雅也, 大塚重人, 松本聰

    日本土壌肥料学会大会  2002.4 

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Research Projects

  • イネとホロビオントを構成する細菌群集はどのように決定・制御されているのか？

    Grant number：24K01651

    2024.4 - 2028.3

    System name：科学研究費助成事業

    Research category：基盤研究(B)

    Awarding organization：日本学術振興会

    原田 直樹, アシルオグル ムハンメットラシット, 鈴木 一輝

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    Grant amount：\18460000 （ Direct Cost: \14200000 、 Indirect Cost：\4260000 ）

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  • Characteristics of rice rhizomicrobiota and carbon/nitrogen dynamics in alkaline paddy fields

    Grant number：23KK0123

    2023.9 - 2027.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Fund for the Promotion of Joint International Research (International Collaborative Research)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\21060000 （ Direct Cost: \16200000 、 Indirect Cost：\4860000 ）

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  • Removal of Cesium in Soil Solution by Zeolite Produced from Fly Ash

    Grant number：21K12290

    2021.4 - 2024.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

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  • Establishment of a separation and quantification method for microplastics in agricultural soil and clarification of the actual contamination status

    Grant number：20K21348

    2020.7 - 2023.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Research category：Grant-in-Aid for Challenging Research (Exploratory)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\6370000 （ Direct Cost: \4900000 、 Indirect Cost：\1470000 ）

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  • Behavior of radiocesium derived from irrigation water in paddy fields and its effects on radioactivity in rice

    Grant number：19H03072

    2019.4 - 2022.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    HARADA NAOKI

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    Grant amount：\17030000 （ Direct Cost: \13100000 、 Indirect Cost：\3930000 ）

    The purpose of this study was to elucidate the mechanisms by which the inflow of irrigation water containing low concentrations of radiocesium (rCs) increases rCs concentration in rice plants near the water inlet. 137Cs in irrigation water was monitored in an agricultural channel in Hamadori district, Fukushima, and the stock of 137Cs in the water vegetation was estimated. The tracer experiment confirmed that dissolved 137Cs migrates from the base of rice plants to the paddy. 137Cs in the irrigation water-derived sediments added to the soil surface was shown to be absorbed by rice plants. A model rice field filled with non-contaminated soil installed in Namie Town showed the two-dimension deposition of 137Cs in irrigation water onto the soil surface. It was concluded that dissolved 137Cs bottomed up rice 137Cs concentration, while suspended 137Cs was transferred to rice plants after deposition on the soil surface near the water inlet, further increasing the rice 137Cs concentration.

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  • Ecological effects and toxicity caused by neonicotinoid pesticides

    Grant number：18H04137

    2018.4 - 2022.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Research category：Grant-in-Aid for Scientific Research (A)

    Awarding organization：Japan Society for the Promotion of Science

    Sekijima Tsuneo

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    Grant amount：\44070000 （ Direct Cost: \33900000 、 Indirect Cost：\10170000 ）

    A summary of research results for each of the ST1-5 subthemes is provided below.
    ST1；The effects of neonicotinoid pesticides on rice paddy communities and the life histories of rice paddy organisms were investigated using paddy mesocosms. ST2；The effects of neonicotinoid pesticides on soil microbial communities and microbial degradation of clothianidin under submerged condition were examined. ST3；The dynamics of Clothianidin in paddy fields and drainage channels were investigated by observation, experiment, and numerical modeling. ST4；It was detected the existence of secondary human exposure and tertiary exposure from breast milk to infants. ST5；We found that fetal exposure of mice to a neonicotinoid pesticide, clothianidin, caused impaired memory formation and altered gene expression in the brain after growing.

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  • Why is the concentration of radiocesium in rice near water inlet high?

    Grant number：26511007

    2014.4 - 2017.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    Yoshikawa Natsuki

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    Grant amount：\5070000 （ Direct Cost: \3900000 、 Indirect Cost：\1170000 ）

    This study aimed at investigating the phenomenon of higher transfer factor of radiocesium from soil to rice plants near water inlets by quantifying spatial distribution of radiocesium in paddy field water, soil and rice plants at 13 experimental paddy fields in Hama-Dori district in Fukushima prefecture, and developing a numerical simulation model. In consequence, it was suggested that the cause of specifically high concentration of radiocesium in rice near the water inlet may be due to the suspended form of radiocesium accumulated near the water inlet but to the dissolved form. Based on this result，densely transplanting near the water inlet to control the diffusion of suspended solid was attempted as a countermeasure for producing low contamination rice. However,this proposal was found to be failure.
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  • Environmental fate of thionated phenylarsenical compounds

    Grant number：25340084

    2013.4 - 2016.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    HARADA NAOKI

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    Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）

    In this study, we isolated Desulfotomaculum acetoxidans strain DEA14, which could anaerobically transform diphenylarsinic acid (DPAA) to diphenylthioarsinic acid (DPTA). A known sulfate-reducing bacterial strain, Desulfovibrio aerotolerans JCM 12613T could also transform DPAA to DPTA. The findings suggest that H2S produced by sulfate-reducing bacteria has a role in thionation of DPAA under anaerobic soil conditions. In addition, we confirmed that other phenylarsenicals, such as diphenylmethylarsine oxide and phenylarsonic acid, could be thionated by reaction with H2S. Uptake of phenylarsenicals in rice was also investigated under flooded conditions. At the harvest, DPTA was found in the soil and root samples. In the leaf, stem and brown rice samples, As(V), DPMAO, PMAA and/or DPAA were detected. The results indicate that arsenical species found in rice vary at different parts and the aerial parts of rice contain no DPTA.

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  • バイオレメディエーションを目指した有機ヒ素化合物の嫌気的微生物変換機構の解明

    2010.4 - 2013.3

    System name：科学研究費助成事業

    Research category：基盤研究(C)

    Awarding organization：日本学術振興会

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    Grant type：Competitive

    Grant amount：\3000000 （ Direct Cost: \2100000 、 Indirect Cost：\900000 ）

    有機ヒ素化合物による土壌汚染は世界各地で大きな問題となっている。その環境動態には微生物の関与が示唆されているが、研究例は非常に少なく未知の点が多い。これまでに我々は、土壌の有機ヒ素汚染物質として最も重要なdiphenylarsinic acid (DPAA)を対象に研究を行い、その好気的分解菌を単離するとともに、さらに還元状態においてはより迅速に微生物変換できることを見出した。そこで本研究では有機ヒ素化合物の還元的微生物変換機構の解明を目的に種々の検討を行う。還元的微生物変換によって毒性が低く土壌吸着しにくい形態のヒ素化合物に変化させることが可能であれば、有機ヒ素汚染土壌に対する新たな修復技術の開発に道を拓くものと期待される。

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  • 稲わら施用量減量による地力低下の程度の確認と水田の冬期湛水化による地力維持の可能性の検討

    2008.8 - 2009.3

    System name：受託研究（一般受託研究）

    Awarding organization：新潟市

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    Grant type：Competitive

    Grant amount：\500000

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  • 植物由来有機物を利用した環境修復の研究

    2008.5 - 2009.3

    System name：共同研究

    Awarding organization：興和株式会社 興和総合科学研究所

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    Grant type：Competitive

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  • アカミミズの飼育法に関する基礎研究

    2007.11 - 2009.3

    System name：共同研究

    Awarding organization：株式会社ミヤトウ野草研究所

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    Grant type：Competitive

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  • 微生物・植物由来バイオマテリアルを用いた環境浄化・改善の研究

    2007.6 - 2008.3

    System name：共同研究

    Awarding organization：興和株式会社 興和総合科学研究所

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    Grant type：Competitive

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Teaching Experience (researchmap)

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Teaching Experience

  • 日本酒学概論I(自然科学)

    2023
    Institution name：新潟大学

  • グローバル農力国際キャリア実習(中期)

    2023
    Institution name：新潟大学

  • グローバル農力国際キャリア実習(長期)

    2023
    Institution name：新潟大学

  • 応用生命・食品科学セミナーIV

    2023
    Institution name：新潟大学

  • スタディ・スキルズAIIa

    2022
    Institution name：新潟大学

  • スタディ・スキルズAIIc

    2022
    -
    2023
    Institution name：新潟大学

  • スタディ・スキルズAIIb

    2022
    -
    2023
    Institution name：新潟大学

  • 統合化学入門

    2022
    Institution name：新潟大学

  • 環境土壌学

    2022
    Institution name：新潟大学

  • 農学入門II

    2021
    Institution name：新潟大学

  • 農学入門I

    2021
    Institution name：新潟大学

  • スタディ・スキルズAIb

    2021
    -
    2022
    Institution name：新潟大学

  • スタディ・スキルズAIa

    2021
    -
    2022
    Institution name：新潟大学

  • グローバル農力

    2021
    -
    2022
    Institution name：新潟大学

  • スタディ・スキルズAIc

    2021
    -
    2022
    Institution name：新潟大学

  • グローバル防災・復興学

    2021
    Institution name：新潟大学

  • 応用生命・食品科学概論

    2021
    Institution name：新潟大学

  • 基礎地学

    2021
    Institution name：新潟大学

  • Topics in Applied Bioresource Chemistry

    2021
    Institution name：新潟大学

  • スタディ・スキルズA b

    2020
    Institution name：新潟大学

  • 資源利用科学特論

    2020
    Institution name：新潟大学

  • 環境土壌学特論

    2019
    -
    2021
    Institution name：新潟大学

  • 農学入門Ⅱ

    2019
    Institution name：新潟大学

  • 農学入門Ⅰ

    2019
    Institution name：新潟大学

  • 土壌学概論

    2018
    Institution name：新潟大学

  • 分析化学(農)

    2018
    Institution name：新潟大学

  • 分析化学実験(農)

    2018
    Institution name：新潟大学

  • 生態系を知る

    2018
    Institution name：新潟大学

  • 環境土壌学

    2018
    -
    2022
    Institution name：新潟大学

  • グローバル農力国際特別研究(長期)

    2018
    Institution name：新潟大学

  • グローバル農力国際インターンシップ(長期)

    2018
    Institution name：新潟大学

  • 環境汚染物質化学

    2018
    Institution name：新潟大学

  • グローバル農力国際特別研究(中期)

    2018
    Institution name：新潟大学

  • 土と水

    2017
    Institution name：新潟大学

  • Topics in Applied Bioresource Chemistry

    2017
    -
    2021
    Institution name：新潟大学

  • 基礎地学

    2017
    -
    2021
    Institution name：新潟大学

  • グローバル農力(短期)

    2017
    -
    2018
    Institution name：新潟大学

  • 応用生命・食品科学セミナーⅡ

    2017
    Institution name：新潟大学

  • 土壌環境学

    2017
    Institution name：新潟大学

  • 応用生物化学セミナーⅡ

    2017
    Institution name：新潟大学

  • グローバル農力国際インターンシップ(中期)

    2016
    -
    2018
    Institution name：新潟大学

  • 応用生命・食品科学概論

    2015
    -
    2021
    Institution name：新潟大学

  • 応用生命・食品科学特論

    2015
    -
    2018
    Institution name：新潟大学

  • 土壌植物資源科学演習 Ⅰ

    2012
    -
    2016
    Institution name：新潟大学

  • 研究発表演習(中間発表)

    2012
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学特定研究BⅡ

    2012
    -
    2015
    Institution name：新潟大学

  • 文献詳読Ⅱ

    2012
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学特定研究BⅠ

    2012
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学セミナーBⅡ

    2012
    -
    2015
    Institution name：新潟大学

  • 文献詳読Ⅰ

    2012
    -
    2015
    Institution name：新潟大学

  • 応用生命・食品科学演習(学会発表)

    2012
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学セミナーBⅠ

    2012
    -
    2015
    Institution name：新潟大学

  • リサーチキャンプ

    2012
    Institution name：新潟大学

  • 現代日本事情

    2012
    Institution name：新潟大学

  • 現代日本論

    2012
    Institution name：新潟大学

  • 自然科学総論Ⅳ

    2012
    Institution name：新潟大学

  • 生命・食料科学セミナーAⅡ

    2012
    Institution name：新潟大学

  • 生命・食料科学特定研究AⅡ

    2012
    Institution name：新潟大学

  • 土壌植物資源科学演習Ⅰ

    2011
    -
    2019
    Institution name：新潟大学

  • 土壌植物資源科学演習Ⅱ

    2011
    -
    2018
    Institution name：新潟大学

  • 土壌化学

    2011
    -
    2013
    Institution name：新潟大学

  • 土壌植物資源科学実験

    2010
    -
    2018
    Institution name：新潟大学

  • 土壌機能解析学特論

    2010
    -
    2017
    Institution name：新潟大学

  • 応用生物化学科インターンシップ

    2010
    -
    2016
    Institution name：新潟大学

  • 応用生物化学概論

    2010
    -
    2016
    Institution name：新潟大学

  • 科学英語演習

    2010
    -
    2016
    Institution name：新潟大学

  • 地学

    2009
    -
    2015
    Institution name：新潟大学

  • スタディ・スキルズA2

    2009
    Institution name：新潟大学

  • 土壌生化学

    2007
    Institution name：新潟大学

  • 分析化学

    2007
    -
    2017
    Institution name：新潟大学

  • 化学実験

    2007
    -
    2017
    Institution name：新潟大学

  • 土壌微生物学特論

    2007
    -
    2016
    Institution name：新潟大学

  • 土壌化学特論

    2007
    -
    2009
    Institution name：新潟大学

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