Updated on 2026/07/08

写真a

 
MITSUI Toshiaki
 
Organization
Social Cooperation Promotion Organization Specially Appointed Professor
Graduate School of Science and Technology Life and Food Sciences Specially Appointed Professor
Faculty of Agriculture Department of Agriculture Specially Appointed Professor
Title
Specially Appointed Professor
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The Best Research Achievement in Research Career

    • 【Papers】 The rice α-amylase glycoprotein is targeted from the Golgi apparatus through the secretory pathway to the plastids.  2009.9

Degree

  • 農学博士 ( 1986.3   名古屋大学 )

Research Areas

  • Life Science / Applied molecular and cellular biology

Research History (researchmap)

  • Niigata University   Specially Appointed Professor

    2024.4

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  • Niigata University   Faculty of Agriculture Department of Agriculture   Professor

    2017.4 - 2024.3

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  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Professor

    2004.4 - 2024.3

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  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry   Professor

    2002.4 - 2017.3

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  • Niigata University   Faculty of Agriculture   Associate Professor (as old post name)

    1993.4 - 2002.3

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  • Niigata University   Faculty of Agriculture   Assistant

    1986.7 - 1993.3

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Research History

  • Niigata University   Life and Food Sciences, Graduate School of Science and Technology   Specially Appointed Professor

    2024.4

  • Niigata University   Department of Agriculture, Faculty of Agriculture   Specially Appointed Professor

    2024.4

  • Niigata University   Social Cooperation Promotion Organization   Specially Appointed Professor

    2024.4

  • Niigata University   Faculty of Agriculture Department of Agriculture   Professor

    2017.4 - 2024.3

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Professor

    2004.4 - 2024.3

  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry   Professor

    2002.4 - 2017.3

  • Niigata University   Faculty of Agriculture   Associate Professor (as old post name)

    1993.4 - 2002.3

  • Niigata University   Faculty of Agriculture   Research Assistant

    1986.7 - 1993.3

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Professional Memberships

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Qualification acquired

  • Chief Person of Radiation Handling （first and second kind）

 

Papers

  • Maternal organellar inheritance and cytosine patterning shape salinity responses in rice hybrids: a multi‐omics perspective

    Murat Aycan, Rasit Asiloglu, Toshiaki Mitsui

    The Plant Journal   126 ( 4 )   2026.5

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    SUMMARY

    Maternally inherited extranuclear genomes (ptDNA and mtDNA) and epigenetic mechanisms are known to affect offspring phenotypes; nevertheless, their integrated function in stress adaptation remains incompletely understood. Classical breeding strategies have mostly ignored these non‐Mendelian influences by focusing exclusively on nuclear genome inheritance. We developed reciprocal rice hybrids from selected salt‐tolerant and salt‐sensitive progenitors and utilized a multi‐omics strategy—comprising whole‐genome sequencing, WGS‐derived cytosine content profiling, transcriptomics, and phenotypic evaluations—to understand the maternal contribution to salinity tolerance of rice offspring. Despite sharing a broadly biparental nuclear genetic background, maternal‐tolerant rice hybrids demonstrated improved performance, including better growth, photosynthesis, ion homeostasis, and yield relative to maternal‐sensitive rice hybrids under salinity stress. Organellar genome sequencing revealed predominantly maternal inheritance. Cytosine profiling indicated CpG and CHG content similarity to the maternal genotype. Transcriptomic data revealed maternal‐aligned expression of stress‐responsive and organelle‐associated genes, consistent with enhanced performance in maternal‐tolerant hybrids. Our results from a multi‐omics approach support a strong association between maternal cytoplasmic inheritance and differential stress responses in rice hybrids. This non‐Mendelian model of stress tolerance, informed by multi‐omics integration, provides innovative insights into maternal regulation of stress resilience in rice and proposes an effective strategy for breeding salt‐tolerant crops in climate‐challenged areas.

    DOI： 10.1111/tpj.70954

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.70954

  • Plant–microbiome interactions are associated with enhanced salinity tolerance and methane emissions in rice

    Murat Aycan, Dorra Fakhet, Pedro J. Picazo, Seda Bodur, Hirohiko Nagano, Rasit Asiloglu, Iker Aranjuelo, Toshiaki Mitsui

    Plant Physiology and Biochemistry   111324 - 111324   2026.4

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.plaphy.2026.111324

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  • Salinity Stress in Rice: Physiological and Molecular Mechanisms with a Focus on the Role of the hst1 Gene and OsRR22 in Enhancing Salt Tolerance

    Murat Aycan, Toshiaki Mitsui

    Crop Design   100129 - 100129   2025.12

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.cropd.2025.100129

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  • Development of an Application Method for Volatile Compounds Derived from Mushroom Fungi Beds as Plant Growth-Promoting Biostimulants

    Clever N. Kanga, Yui Okisaka, Shigeru Hanamata, Daijiro Ueda, Tsutomu Sato, Toshiaki Mitsui, Kimiko Itoh

    Methods and Protocols   8 ( 2 )   29 - 29   2025.3

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Volatile compounds (VCs) from fungi can promote plant growth, but their application methods are limited. Edible mushroom fungi beds (FBs) provide a readily available alternative source of fungal VCs, although their biostimulatory functions remain unvalidated. In this study, a novel, non-contact exposure method for applying VCs emitted from FBs to rice seedlings was developed. This marks the first evaluation of mushroom FBs as a direct source of bioactive VCs for plant growth promotion. Volatiles from two different edible mushroom FBs promoted shoot growth and increased biomass for rice seedlings. VCs from shiitake FBs significantly increased biomass by 67.4% while VCs from enokitake FBs by 39.5% compared to the control. The biomass-increasing effects were influenced by the quantity of shiitake FBs applied, with significant increases at 15 g, 30 g and 60 g applications. The VCs effects remained significant even when the FBs were covered with two types of gas-permeable polymer film. Chemical analysis of VCs from FBs identified several organic compounds and subsequent bioassays using synthetic VCs determined key bioactive VCs contributing to biomass increase at specific concentrations. This study presents a utilization method of waste mushroom FBs as sustainable, scalable, and cost-effective agricultural biostimulants.

    DOI： 10.3390/mps8020029

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  • Integrating Biofertilizers with Organic Fertilizers Enhances Photosynthetic Efficiency and Upregulates Chlorophyll-Related Gene Expression in Rice

    Pretty Mthiyane, Murat Aycan, Toshiaki Mitsui

    Sustainability   16 ( 21 )   9297 - 9297   2024.10

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Biofertilizers offer a sustainable method for improving rice growth and productivity, yet their effects on the interaction between plant growth, photosynthetic activity, and gene expression remain under-researched. This study examines how biofertilizer influences rice physiology, focusing on photosynthetic regulation and expression of chlorophyll-related genes. Eight fertilizer treatments were applied: control (CNT), biofertilizer (BF), deactivated biofertilizer (DABF), rice straw (RS), rice straw with biofertilizer (RS+BF), organic fertilizer (OF), organic fertilizer with biofertilizer (OF+BF), and inorganic fertilizer (IOF). Plant height, tiller number, SPAD, NDVI, chlorophyll content, and photosynthesis rates were measured, while gene expression analysis was conducted using RT-qPCR. The OF+BF treatment produced the most significant results, leading to a 31% increase in plant height, a 135% increase in tiller number, and a 42% increase in chlorophyll content (SPAD values) compared to the control. Additionally, OF+BF enhanced photosynthetic efficiency by 74%, with the highest net photosynthetic rate of 48.23 μmol CO2 m⁻2 s⁻1. Gene expression analysis revealed that OF+BF upregulated key photosynthesis-related genes, such as OsChlD and OsCHLM, showing a 70% and 90% increase in expression. These findings highlight the potential of integrating biofertilizers with organic fertilizers to sustainably boost rice growth and productivity, contributing to global food security and climate change mitigation.

    DOI： 10.3390/su16219297

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  • Regulation of Common Early and Late Stress Responses in Rice by Transcriptional and Antioxidant Mechanisms Under Salt Stress

    Murat Aycan, Toshiaki Mitsui

    Journal of Plant Growth Regulation   2024.7

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00344-024-11409-5

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    Other Link： https://link.springer.com/article/10.1007/s00344-024-11409-5/fulltext.html

  • Exogenous proline suppresses endogenous proline and proline-production genes but improves the salinity tolerance capacity of salt-sensitive rice by stimulating antioxidant mechanisms and photosynthesis

    Yunus Emre Koc, Murat Aycan, Toshiaki Mitsui

    Plant Physiology and Biochemistry   108914 - 108914   2024.7

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.plaphy.2024.108914

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  • Strategic Advancements in Rice Cultivation: Combating Heat Stress through Genetic Innovation and Sustainable Practices—A Review Reviewed

    Pretty Mthiyane, Murat Aycan, Toshiaki Mitsui

    Stresses   4   452 - 480   2024.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/stresses4030030

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  • Transgenerational salinity tolerance plasticity in rice: Unraveling nongenetic phenotypic modifications and environmental influences

    Murat Aycan, Lutfun Nahar, Marouane Baslam, Toshiaki Mitsui

    Journal of Experimental Botany   2024.5

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    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Transgenerational plasticity in plants enables rapid adaptation to environmental changes, allowing organisms and their offspring to adapt to the environment without altering their underlying DNA. In this study, we investigated the plasticity of transgenerational salinity tolerance of rice plants using a reciprocal transplant experimental strategy. Our aim was to assess whether nongenetic environment-induced phenotypic modifications and transgenerational salinity affect the salinity tolerance of progeny while excluding nuclear genomic factors for two generations. Using salt-tolerant and salt-sensitive rice genotypes, we observed that the parentally salt-stressed salt-sensitive genotype displayed greater growth performance, photosynthetic activity, yield performance, and transcriptional responses than did the parentally nonstressed salt-sensitive plants under salt stress conditions. Surprisingly, salt stress-exposed salt-tolerant progeny did not exhibit as much salinity tolerance as salt stress-exposed salt-sensitive progeny under salt stress. Our findings indicate that the phenotypes of offspring plants differed based on the environment experienced by their ancestors, resulting in heritable transgenerational phenotypic modifications in salt-sensitive genotypes via maternal effects. These results elucidated the mechanisms underlying transgenerational plasticity in salinity tolerance, providing valuable insights into how plants respond to changing environmental conditions.

    DOI： 10.1093/jxb/erae211

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  • Assessing Contrasting Wheat (Triticum aestivum L.) Cultivars Responsiveness to Salinity at the Seedling Stage and Screening of Tolerance Marker Traits

    Murat Aycan, Marouane Baslam, Toshiaki Mitsui, Mustafa Yildiz

    Journal of Plant Growth Regulation   2024.4

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00344-024-11295-x

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    Other Link： https://link.springer.com/article/10.1007/s00344-024-11295-x/fulltext.html

  • Self-Defense Mechanism in Rice to Salinity: Proline Reviewed

    Yunus Emre Koc, Murat Aycan, Toshiaki Mitsui

    J   7   103 - 115   2024.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/j7010006

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  • The Promising B−Type Response Regulator hst1 Gene Provides Multiple High Temperature and Drought Stress Tolerance in Rice

    Ermelinda Maria Lopes Lopes Hornai, Murat Aycan, Toshiaki Mitsui

    International Journal of Molecular Sciences   25 ( 4 )   2385 - 2385   2024.2

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    High temperatures, drought, and salt stresses severely inhibit plant growth and production due to the effects of climate change. The Arabidopsis ARR1, ARR10, and ARR12 genes were identified as negative salt and drought stress regulators. However, in rice, the tolerance capacity of the hst1 gene, which is orthologous to the ARR1, ARR10, and ARR12 genes, to drought and multiple high temperature and drought stresses remains unknown. At the seedling and reproductive stages, we investigated the drought (DS) high temperature (HT) and multiple high temperature and drought stress (HT+DS) tolerance capacity of the YNU31−2−4 (YNU) genotype, which carries the hst1 gene, and its nearest genomic relative Sister Line (SL), which has a 99% identical genome without the hst1 gene. At the seedling stage, YNU demonstrated greater growth, photosynthesis, antioxidant enzyme activity, and decreased ROS accumulation under multiple HT+DS conditions. The YNU genotype also demonstrated improved yield potential and grain quality due to higher antioxidant enzyme activity and lower ROS generation throughout the reproductive stage under multiple HT+DS settings. Furthermore, for the first time, we discovered that the B−type response regulator hst1 gene controls ROS generation and antioxidant enzyme activities by regulating upstream and downstream genes to overcome yield reduction under multiple high temperatures and drought stress. This insight will help us to better understand the mechanisms of high temperature and drought stress tolerance in rice, as well as the evolution of tolerant crops that can survive increased salinity to provide food security during climate change.

    DOI： 10.3390/ijms25042385

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  • Functional traits of field-droughted contrasting rice genotypes reveal multiple independent genomic adaptations and metabolic responses

    Marouane Baslam, Takeshi Takamatsu, Murat Aycan, Dorra Fakhet, Fatima Zahra Rezzouk, Bertrand Gakière, José Luis Araus, Iker Aranjuelo, Toshiaki Mitsui

    Environmental and Experimental Botany   215   105483 - 105483   2023.11

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.envexpbot.2023.105483

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  • Tolerance with High Yield Potential Is Provided by Lower Na+ Ion Accumulation and Higher Photosynthetic Activity in Tolerant YNU31-2-4 Rice Genotype under Salinity and Multiple Heat and Salinity Stress. International journal

    Lutfun Nahar, Murat Aycan, Ermelinda Maria Lopes Hornai, Marouane Baslam, Toshiaki Mitsui

    Plants (Basel, Switzerland)   12 ( 9 )   2023.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    The yield-reduction effect of abiotic stressors such as salinity and heat stresses with the growing world population threatens food security. Although adverse effects of salinity and heat stress on plant growth and production parameters have been documented, in nature, abiotic stresses occur sequentially or simultaneously. In this study, the stress tolerance and yield capacity of Yukinkomai, YNU31-2-4, and YNU SL rice genotypes tested under control (26 °C, 0 mM NaCl), salinity (26 °C, 75 mM NaCl), heat (31 °C, 0 mM NaCl), and heat and salinity (31 °C, 75 mM NaCl) stress combinations at vegetative and reproductive stages with six different scenarios. The results show that salinity and the heat and salinity combination stresses highly reduce plant growth performance and yield capacity. Heat stress during reproduction does not affect the yield but reduces the grain quality. The YNU31-2-4 genotype performs better under heavy salt and heat and salinity stress then the Yukinkomai and YNU SL genotypes. YNU31-2-4 genotypes accumulate less Na+ and more K+ under salt and multiple stresses. In the YNU31-2-4 genotype, low Na+ ion accumulation increases photosynthetic activity and pigment deposition, boosting the yield. Stress lowers the glucose accumulation in dry seeds, but the YNU31-2-4 genotype has a higher glucose accumulation.

    DOI： 10.3390/plants12091910

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  • Genomics-Assisted Use of Genetic Resources for Environmentally Adaptive Plant Breeding

    Murat Aycan, Marouane Baslam, Toshiaki Mitsui, Mustafa Yildiz

    Cereal Crops   157 - 174   2023.4

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    Publishing type：Part of collection (book)   Publisher：CRC Press  

    DOI： 10.1201/9781003250845-8

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  • B-type response regulator hst1 controls salinity tolerance in rice by regulating transcription factors and antioxidant mechanisms

    Murat Aycan, Lutfun Nahar, Marouane Baslam, Toshiaki Mitsui

    Plant Physiology and Biochemistry   2023.2

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.plaphy.2023.02.008

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  • The TaGSK1, TaSRG, TaPTF1, and TaP5CS Gene Transcripts Confirm Salinity Tolerance by Increasing Proline Production in Wheat (Triticum aestivum L.)

    Murat Aycan, Marouane Baslam, Toshiaki Mitsui, Mustafa Yildiz

    Plants   11 ( 23 )   3401 - 3401   2022.12

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Salinity is an abiotic stress factor that reduces yield and threatens food security in the world’s arid and semi-arid regions. The development of salt-tolerant genotypes is critical for mitigating yield losses, and this journey begins with the identification of sensitive and tolerant plants. Numerous physiologic and molecular markers for detecting salt-tolerant wheat genotypes have been developed. One of them is proline, which has been used for a long time but has received little information about proline-related genes in wheat genotypes. In this study, proline content and the expression levels of proline-related genes (TaPTF1, TaDHN, TaSRG, TaSC, TaPIMP1, TaMIP, TaHKT1;4, TaGSK, TaP5CS, and TaMYB) were examined in sensitive, moderate, and tolerant genotypes under salt stress (0, 50, 150, and 250 mM NaCl) for 0, 12, and 24 h. Our results show that salt stress increased the proline content in all genotypes, but it was found higher in salt-tolerant genotypes than in moderate and sensitive genotypes. The salinity stress increased gene expression levels in salt-tolerant and moderate genotypes. While salt-stress exposure for 12 and 24 h had a substantial effect on gene expression in wheat, TaPTF1, TaPIMP1, TaMIP, TaHKT1;4, and TaMYB genes were considerably upregulated in 24 h. The salt-tolerant genotypes showed a higher positive interaction than a negative interaction. The TaPTF1, TaP5CS, TaGSK1, and TaSRG genes were found to be more selective than the other analyzed genes under salt-stress conditions. Despite each gene’s specific function, increasing proline biosynthesis functioned as a common mechanism for separating salt tolerance from sensitivity.

    DOI： 10.3390/plants11233401

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  • Evaluation of Young Date Palm Tolerance to Salinity Stress under Arbuscular Mycorrhizal Fungi and Compost Application

    Mohamed Ait-El-Mokhtar, Raja Ben-Laouane, Abderrahim Boutasknit, Mohamed Anli, Abdessamad Fakhech, Youssef Ait-Rahou, Toshiaki Mitsui, Said Wahbi, Marouane Baslam, Abdelilah Meddich

    Environmental Sciences Proceedings   2022.6

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/environsciproc2022016015

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  • Impact of Single and Combined Salinity and High-Temperature Stresses on Agro-Physiological, Biochemical, and Transcriptional Responses in Rice and Stress-Release

    Lutfun Nahar, Murat Aycan, Shigeru Hanamata, Marouane Baslam, Toshiaki Mitsui

    Plants   11 ( 4 )   501 - 501   2022.2

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Here, for the first time, we aimed to identify in rice the key mechanisms and processes underlying tolerance to high-temperature (HT) or salt stress (SS) alone, the co-occurrence of both stresses, and recovery using physiological and biochemical measurements and gene expression analysis. We also investigated whether recovery from the two stressors depended on the relative intensities/relief of each stressor. Wild type (‘Yukinkomai’) rice plants were found to be more susceptible to salinity or heat applied individually. SS leads to a depletion of cellular water content, higher accumulation of Na+, and alterations in photosynthetic pigments. The stress-tolerant cultivar ‘YNU31-2-4’ (YNU) displayed a lower Na+/K+ ratio, higher water content in cells and improved photosynthetic traits, antioxidant system, and expression of defence genes. Strikingly, the SS + HT combination provided a significant level of protection to rice plants from the effects of SS alone. The expression pattern of a selected set of genes showed a specific response and dedicated pathways in plants subjected to each of the different stresses, while other genes were explicitly activated when the stresses were combined. Aquaporin genes were activated by SS, while stress-related (P5CS, MSD1, HSPs, and ions transporters) genes were shaped by HT. Hierarchical clustering and principal component analyses showed that several traits exhibited a gradually aggravating effect as plants were exposed to the combined stresses and identified heat as a mitigating factor, clearly separating heat + salt-stressed from salt-non-heat-stressed plants. Furthermore, seedling recovery was far more dependent on the relative intensities of stressors and cultivars, demonstrating the influence of one stressor over another upon stress-release. Taken together, our data show the uniqueness and complexity of the physiological and molecular network modules used by rice plants to respond to single and combined stresses and recovery.

    DOI： 10.3390/plants11040501

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  • The Native Arbuscular Mycorrhizal Fungi and Vermicompost-Based Organic Amendments Enhance Soil Fertility, Growth Performance, and the Drought Stress Tolerance of Quinoa. International journal

    Wissal Benaffari, Abderrahim Boutasknit, Mohamed Anli, Mohamed Ait-El-Mokhtar, Youssef Ait-Rahou, Raja Ben-Laouane, Hela Ben Ahmed, Toshiaki Mitsui, Marouane Baslam, Abdelilah Meddich

    Plants (Basel, Switzerland)   11 ( 3 )   2022.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    The present study aimed to determine the effects of biostimulants on the physicochemical parameters of the agricultural soil of quinoa under two water regimes and to understand the mode of action of the biostimulants on quinoa for drought adaptation. We investigated the impact of two doses of vermicompost (5 and 10 t/ha) and arbuscular mycorrhizal fungi applied individually, or in joint application, on attenuating the negative impacts of water shortage and improving the agro-physiological and biochemical traits of quinoa, as well as soil fertility, under two water regimes (well-watered and drought stress) in open field conditions. Exposure to drought decreased biomass, leaf water potential, and stomatal conductance, and increased malondialdehyde and hydrogen peroxide content. Mycorrhiza and/or vermicompost promoted plant growth by activating photosynthesis machinery and nutrient assimilation, leading to increased total soluble sugars, proteins, and antioxidant enzyme activities in the leaf and root. After the experiment, the soil's total organic matter, phosphorus, nitrogen, calcium, and soil glomalin content improved by the single or combined application of mycorrhiza and vermicompost. This knowledge suggests that the combination of mycorrhiza and vermicompost regulates the physiological and biochemical processes employed by quinoa in coping with drought and improves the understanding of soil-plant interaction.

    DOI： 10.3390/plants11030393

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  • Root Reinforcement Improved Performance, Productivity, and Grain Bioactive Quality of Field-Droughted Quinoa (Chenopodium quinoa). International journal

    Salma Toubali, Mohamed Ait-El-Mokhtar, Abderrahim Boutasknit, Mohamed Anli, Youssef Ait-Rahou, Wissal Benaffari, Hela Ben-Ahmed, Toshiaki Mitsui, Marouane Baslam, Abdelilah Meddich

    Frontiers in plant science   13   860484 - 860484   2022

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    Language：English   Publishing type：Research paper (scientific journal)  

    Modern agriculture is facing multiple and complex challenges and has to produce more food and fiber to feed a growing population. Increasingly volatile weather and more extreme events such as droughts can reduce crop productivity. This implies the need for significant increases in production and the adoption of more efficient and sustainable production methods and adaptation to climate change. A new technological and environment-friendly management technique to improve the tolerance of quinoa grown to maturity is proposed using native microbial biostimulants (arbuscular mycorrhizal fungi; AMF) alone, in the consortium, or in combination with compost (Comp) as an organic matter source under two water treatments (normal irrigation and drought stress (DS)). Compared with controls, growth, grain yield, and all physiological traits under DS were significantly decreased while hydrogen peroxide, malondialdehyde, and antioxidative enzymatic functions were significantly increased. Under DS, biofertilizer application reverted physiological activities to normal levels and potentially strengthened quinoa's adaptability to water shortage as compared to untreated plants. The dual combination yielded a 97% improvement in grain dry weight. Moreover, the effectiveness of microbial and compost biostimulants as a biological tool improves grain quality and limits soil degradation under DS. Elemental concentrations, particularly macronutrients, antioxidant potential (1,1-diphenyl-2-picrylhydrazyl radical scavenging activity), and bioactive compounds (phenol and flavonoid content), were accumulated at higher levels in biofertilizer-treated quinoa grain than in untreated controls. The effects of AMF + Comp on post-harvest soil fertility traits were the most positive, with significant increases in total phosphorus (47%) and organic matter (200%) content under drought conditions. Taken together, our data demonstrate that drought stress strongly influences the physiological traits, yield, and quality of quinoa. Microbial and compost biostimulation could be an effective alternative to ensure greater recovery capability, thereby maintaining relatively high levels of grain production. Our study shows that aboveground stress responses in quinoa can be modulated by signals from the microbial/compost-treated root. Further, quinoa grains are generally of higher nutritive quality when amended and inoculated with AMF as compared to non-inoculated and compost-free plants.

    DOI： 10.3389/fpls.2022.860484

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  • Assemblage of indigenous arbuscular mycorrhizal fungi and green waste compost enhance drought stress tolerance in carob (Ceratonia siliqua L.) trees. International journal

    Abderrahim Boutasknit, Marouane Baslam, Mohamed Ait-El-Mokhtar, Mohamed Anli, Raja Ben-Laouane, Youssef Ait-Rahou, Toshiaki Mitsui, Allal Douira, Cherkaoui El Modafar, Said Wahbi, Abdelilah Meddich

    Scientific reports   11 ( 1 )   22835 - 22835   2021.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    In the current study, an eco-friendly management technology to improve young carob (Ceratonia siliqua L.) tree tolerance to water deficit was set up by using single or combined treatments of arbuscular mycorrhizal fungi (AMF) and/or compost (C). Two groups of young carob have been installed: (i) carob cultivated under well-watered conditions (WW; 70% field capacity (FC)) and (ii) where the plants were drought-stressed (DS; 35% FC) during 2, 4, 6, and 8 months. The effect of used biofertilizers on the course of growth, physiological (photosynthetic traits, water status, osmolytes, and mineral content), and biochemical (hydrogen peroxide (H2O2), oxidative damage to lipids (malondialdehyde (MDA), and membrane stability (MS)) traits in response to short- and long-term droughts were assessed. The dual application of AMF and C (C + AMF) boosted growth, physiological and biochemical parameters, and nutrient uptake in carob under WW and DS. After eight months, C + AMF significantly enhanced stomatal conductance by 20%, maximum photochemical efficiency of PSII by 7%, leaf water potential by 23%, chlorophyll and carotenoid by 40%, plant uptake of mineral nutrients (P by 75%, N by 46%, K+ by 35%, and Ca2+ by 40%), concentrations of soluble sugar by 40%, and protein content by 44% than controls under DS conditions. Notably, C + AMF reduced the accumulation of H2O2 and MDA content to a greater degree and increased MS. In contrast, enzyme activities (superoxide dismutase, catalase, peroxidase, and polyphenoloxidase) significantly increased in C + AMF plants under DS. Overall, our findings suggest that the pairing of C + AMF can mediate superior drought tolerance in young carob trees by increasing leaf stomatal conductance, cellular water content, higher solute concentration, and defense response against oxidative damage during the prolonged period of DS.

    DOI： 10.1038/s41598-021-02018-3

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  • Direct contribution of the maternal genotype on the transgenerational salinity tolerance in wheat (Triticum aestivum L.)

    Murat Aycan, Marouane Baslam, Bayram Ozdemir, Rasit Asiloglu, Toshiaki Mitsui, Mustafa Yildiz

    Environmental and Experimental Botany   104648 - 104648   2021.9

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.envexpbot.2021.104648

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  • Development of new high-salt tolerant bread wheat (Triticum aestivum L.) genotypes and insight into the tolerance mechanisms

    Murat Aycan, Marouane Baslam, Rasit Asiloglu, Toshiaki Mitsui, Mustafa Yildiz

    Plant Physiology and Biochemistry   166   314 - 327   2021.9

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.plaphy.2021.05.041

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  • Effects of Temperature and Duration of Soaking Dormant Rice Seed on Germination and α-Amylase Expression

    Shigeto Itayagoshi, Masakazu Iwatsu, Seiichi Mizusawa, Rozan Fukushima, Hiroshi Shibukawa, Toshiaki Mitsui

    Japanese Journal of Crop Science   90 ( 3 )   269 - 276   2021.7

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    Publishing type：Research paper (scientific journal)   Publisher：Crop Science Society of Japan  

    DOI： 10.1626/jcs.90.269

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  • Management of puddled soil through organic amendments for post-rice mungbean

    Md Arafat Hossain, Masud Rana, S M Hisam Al Rabbi, Toshiaki Mitsui

    Asian Journal of Agriculture and Biology   2021 ( 1 )   2021.1

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    DOI： 10.35495/ajab.2020.04.255

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  • Recent Advances in Carbon and Nitrogen Metabolism in C3 Plants

    Marouane Baslam, Toshiaki Mitsui, Kuni Sueyoshi, Takuji Ohyama

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   22 ( 1 )   2021.1

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  • Potential of Native Arbuscular Mycorrhizal Fungi, Rhizobia, and/or Green Compost as Alfalfa (Medicago sativa) Enhancers under Salinity

    Raja Ben-Laouane, Marouane Baslam, Mohamed Ait-El-Mokhtar, Mohamed Anli, Abderrahim Boutasknit, Youssef Ait-Rahou, Salma Toubali, Toshiaki Mitsui, Khalid Oufdou, Said Wahbi, Abdelilah Meddich

    MICROORGANISMS   8 ( 11 )   2020.11

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  • Biofertilizers as Strategies to Improve Photosynthetic Apparatus, Growth, and Drought Stress Tolerance in the Date Palm

    Mohamed Anli, Marouane Baslam, Abdelilah Tahiri, Anas Raklami, Sarah Symanczik, Abderrahim Boutasknit, Mohamed Ait-El-Mokhtar, Raja Ben-Laouane, Salma Toubali, Youssef Ait Rahou, Mustapha Ait Chitt, Khalid Oufdou, Toshiaki Mitsui, Mohamed Hafidi, Abdelilah Meddich

    FRONTIERS IN PLANT SCIENCE   11   2020.10

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    DOI： 10.3389/fpls.2020.516818

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  • Alleviation of Detrimental Effects of Salt Stress on Date Palm (Phoenix dactylifera L.) by the Application of Arbuscular Mycorrhizal Fungi and/or Compost

    Mohamed Ait-El-Mokhtar, Marouane Baslam, Raja Ben-Laouane, Mohamed Anli, Abderrahim Boutasknit, Toshiaki Mitsui, Said Wahbi, Abdelilah Meddich

    FRONTIERS IN SUSTAINABLE FOOD SYSTEMS   4   2020.9

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  • Photosynthesis in a Changing Global Climate: Scaling Up and Scaling Down in Crops

    Marouane Baslam, Toshiaki Mitsui, Michael Hodges, Eckart Priesack, Matthew T. Herritt, Iker Aranjuelo, Alvaro Sanz-Saez

    FRONTIERS IN PLANT SCIENCE   11   2020.7

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  • Evaluation of the palatability and biofunctionality of brown rice germinated in red onion solution

    Sumiko Nakamura, Kentaro Kaneko, Toshiaki Mitsui, Ken'ichi Ohtsubo

    CEREAL CHEMISTRY   97 ( 4 )   836 - 848   2020.7

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    DOI： 10.1002/cche.10305

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  • Functional Analysis of Rice Long-Chain Acyl-CoA Synthetase 9 (OsLACS9) in the Chloroplast Envelope Membrane Reviewed

    Aya Kitajima-Koga, Marouane Baslam, Yuuki Hamada, Namiko Ito, Tomoko Taniuchi, Takeshi Takamatsu, Kazusato Oikawa, Kentaro Kaneko, Toshiaki Mitsui

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   21 ( 6 )   2223 - 2223   2020.3

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    DOI： 10.3390/ijms21062223

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  • Arbuscular Mycorrhizal Fungi Mediate Drought Tolerance and Recovery in Two Contrasting Carob (Ceratonia siliqua L.) Ecotypes by Regulating Stomatal, Water Relations, and (In)Organic Adjustments Reviewed

    Abderrahim Boutasknit, Marouane Baslam, Mohamed Ait-El-Mokhtar, Mohamed Anli, Raja Ben-Laouane, Allal Douira, Cherkaoui El Modafar, Toshiaki Mitsui, Said Wahbi, Abdelilah Meddich

    PLANTS-BASEL   9 ( 1 )   80 - 80   2020.1

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    DOI： 10.3390/plants9010080

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  • Proteomic for Quality: Mining the Proteome as a Strategy to Elucidate the Protein Complex Applied for Quality Improvement Reviewed

    Marouane Baslam, Toshiaki Mitsui

    The Future of Rice Demand: Quality Beyond Productivity   473 - 494   2020

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    DOI： 10.1007/978-3-030-37510-2_20

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  • iTRAQ-Based Proteomic Analysis of Rice Grains

    Marouane Baslam, Kentaro Kaneko, Toshiaki Mitsui

    Methods in Molecular Biology   2139   405 - 414   2020

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    DOI： 10.1007/978-1-0716-0528-8_29

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  • Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation Reviewed International journal

    Yuri SERA, Shigeru HANAMATA, Shingo SAKAMOTO, Seijiro ONO, Kentaro KANEKO, Yuudai MITSUI, Tomoko KOYANO, Naoko FUJITA, Ai SASOU, Takehiro MASUMURA, Hikaru SAJI, Ken-Ichi Nonomura, Nobutaka MITSUDA, Toshiaki MITSUI, Takamitsu KURUSU, Kazuyuki KUCHITSU

    Scientific Reports   9 ( 18544 )   1 - 14   2019.12

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    Autophagy plays crucial roles in the recycling of metabolites, and is involved in many developmental processes. Rice mutants defective in autophagy are male sterile due to immature pollens, indicating its critical role in pollen development. However, physiological roles of autophagy during seed maturation had remained unknown. We here found that seeds of the rice autophagy-deficient mutant Osatg7-1, that produces seeds at a very low frequency in paddy fields, are smaller and show chalky appearance and lower starch content in the endosperm at the mature stage under normal growth condition. We comprehensively analyzed the effects of disruption of autophagy on biochemical properties, proteome and seed quality, and found an abnormal activation of starch degradation pathways including accumulation of α-amylases in the endosperm during seed maturation in Osatg7-1. These results indicate critical involvement of autophagy in metabolic regulation in the endosperm of rice, and provide insights into novel autophagy-mediated regulation of starch metabolism during seed maturation.

    DOI： 10.1038/s41598-019-54361-1

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  • Utilization of the Proteome Data Deposited in SRMAtIas for Validating the Existence of the Human Missing Proteins in GPM Reviewed

    Amr Elguoshy, Yoshitoshi Hirao, Keiko Yamamoto, Bo Xu, Naohiko Kinoshita, Toshiaki Mitsui, Tadashi Yamamoto

    JOURNAL OF PROTEOME RESEARCH   18 ( 12 )   4197 - 4205   2019.12

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  • Plant responses to fungal volatiles involve global posttranslational thiol redox proteome changes that affect photosynthesis Reviewed

    Kinia Ameztoy, Marouane Baslam, Angela Maria Sanchez-Lopez, Francisco Jose Munoz, Abdellatif Bahaji, Goizeder A. Magro, Pablo Garcia-Gomez, Edurne Baroja-Fernandez, Nuria De Diego, Jan F. Humplik, Lydia Ugena, Lukas Spichal, Karel Dolezal, Kentaro Kaneko, Toshiaki Mitsui, Francisco Javier Cejudo, Javier Pozueta-Romero

    PLANT CELL AND ENVIRONMENT   42 ( 9 )   2627 - 2644   2019.9

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    DOI： 10.1111/pce.13601

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  • Optimized Nuclear Pellet Method for Extracting Next-Generation Sequencing Quality Genomic DNA from Fresh Leaf Tissue Reviewed

    Md Masud RANA, Murat AYCAN, Takeshi TAKAMATSU, Kentaro KANEKO, Toshiaki MITSUI, Kimiko ITOH

    Method and protocols   2 ( 2 )   1 - 11   2019.6

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    Next-generation sequencing (NGS) is a revolutionary advancement allowing large-scale discovery of functional molecular markers that has many applications, including plant breeding. High-quality genomic DNA (gDNA) is a prerequisite for successful NGS library preparation and sequencing; however, few reliable protocols to obtain such plant gDNA exist. A previously reported nuclear pellet (NP) method enables extraction of high-yielding gDNA from fresh leaf tissue of maize (Zea mays L.), but the quality does not meet the stringent requirements of NGS. In this study, we optimized the NP method for whole-genome sequencing of rice (Oryza sativa L.) through the integration of simple purification steps. The optimized NP method relied on initial nucleus enrichment, cell lysis, extraction, and subsequent gDNA purification buffers. The purification steps used proteinase K, RNase A, phenol/chloroform/isoamyl alcohol (25:24:1), and chloroform/isoamyl alcohol (24:1) treatments for protein digestion and RNA, protein, and phenol removal, respectively. Our data suggest that this optimized NP method allowed extraction of consistently high-yielding and high-quality undegraded gDNA without contamination by protein and RNA. Moreover, the extracted gDNA fulfilled the quality metrics of NGS library preparation for the Illumina HiSeq X Ten platform by the TruSeq DNA PCR-Free Library Prep Kit (Illumina). We provide a reliable step-by-step guide to the extraction of high-quality gDNA from fresh leaf tissues of rice for molecular biologists with limited resources.

    DOI： 10.3390/mps2020054

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  • Salt Tolerance Improvement in Rice through Efficient SNP Marker-Assisted Selection Coupled with Speed-Breeding. Reviewed International journal

    Md Masud Rana, Takeshi Takamatsu, Marouane Baslam, Kentaro Kaneko, Kimiko Itoh, Naoki Harada, Toshie Sugiyama, Takayuki Ohnishi, Tetsu Kinoshita, Hiroki Takagi, Toshiaki Mitsui

    International journal of molecular sciences   20 ( 10 )   1 - 22   2019.5

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    Salinity critically limits rice metabolism, growth, and productivity worldwide. Improvement of the salt resistance of locally grown high-yielding cultivars is a slow process. The objective of this study was to develop a new salt-tolerant rice germplasm using speed-breeding. Here, we precisely introgressed the hst1 gene, transferring salinity tolerance from "Kaijin" into high-yielding "Yukinko-mai" (WT) rice through single nucleotide polymorphism (SNP) marker-assisted selection. Using a biotron speed-breeding technique, we developed a BC3F3 population, named "YNU31-2-4", in six generations and 17 months. High-resolution genotyping by whole-genome sequencing revealed that the BC3F2 genome had 93.5% similarity to the WT and fixed only 2.7% of donor parent alleles. Functional annotation of BC3F2 variants along with field assessment data indicated that "YNU31-2-4" plants carrying the hst1 gene had similar agronomic traits to the WT under normal growth condition. "YNU31-2-4" seedlings subjected to salt stress (125 mM NaCl) had a significantly higher survival rate and increased shoot and root biomasses than the WT. At the tissue level, quantitative and electron probe microanalyzer studies indicated that "YNU31-2-4" seedlings avoided Na+ accumulation in shoots under salt stress. The "YNU31-2-4" plants showed an improved phenotype with significantly higher net CO2 assimilation and lower yield decline than WT under salt stress at the reproductive stage. "YNU31-2-4" is a potential candidate for a new rice cultivar that is highly tolerant to salt stress at the seedling and reproductive stages, and which might maintain yields under a changing global climate.

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  • Use of organic and biological fertilizers as strategies to improve crop biomass, yields and physicochemical parameters of soil

    Abdelilah Meddich, Khalid Oufdou, Abderrahim Boutasknit, Anas Raklami, Abdelilah Tahiri, Raja Ben-Laouane, Mohamed Ait-El-Mokhtar, Mohamed Anli, Toshiaki Mitsui, Said Wahbi, Marouane Baslam

    Nutrient Dynamics for Sustainable Crop Production   247 - 288   2019.1

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    DOI： 10.1007/978-981-13-8660-2_9

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  • Proteomics Analysis Reveals Non-Controlled Activation of Photosynthesis and Protein Synthesis in a Rice npp1 Mutant under High Temperature and Elevated CO2 Conditions Reviewed

    Takuya Inomata, Marouane Baslam, Takahiro Masui, Tsutomu Koshu, Takeshi Takamatsu, Kentaro Kaneko, Javier Pozueta-Romero, Toshiaki Mitsui

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   19 ( 9 )   1 - 18   2018.9

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  • Novel molecular and cell biological insights into function of rice α-amylase Reviewed

    Toshiaki Mitsui, Akihito Ochiai, Hiromoto Yamakawa, Kentaro Kaneko, Aya Kitajima-Koga, Marouane Baslam

    Amylase   2 ( 1 )   30 - 38   2018.7

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    <title>Abstract</title>
    α-Amylases have been of interest in diverse fields for many years because of their importance in basic biology, agriculture, and industry. Starch hydrolysis in plants has been studied extensively in germinating cereal seeds. It is generally accepted that α-amylases are secretory enzymes with a pivotal role in the breakdown of starch reserves in the endosperm. Intriguingly, however, recent investigations reveal that some α-amylases degrade starch in the plastids of living cells. The recent solving of the crystal structure of rice AmyI-1 isoform shows that the binding pocket of starch binding site 1 situated outside of the active site cleft interacts with the substances other than oligosaccharides. These findings provided novel insights into structural and cell biological aspects of α-amylase functions in intracellular transport, organelle targeting, and organ-specific actions. Under global warming, abnormal high temperatures during rice grain filling increase grain chalkiness, resulting in yield loss. Intensive “omics” analyses of developing caryopses and mature grains grown under heat stress showed the downregulation of starch synthesis enzymes and the upregulation of α-amylases. Transgenic studies using ectopic overexpression and suppression of α-amylase revealed that α-amylase is a key factor in grain chalkiness. Here we discuss unique new functions of α-amylase in rice cells.

    DOI： 10.1515/amylase-2018-0004

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  • Optimized Method of Extracting Rice Chloroplast DNA for High-Quality Plastome Resequencing and de Novo Assembly Reviewed

    Takeshi Takamatsu, Marouane Baslam, Takuya Inomata, Kazusato Oikawa, Kimiko Itoh, Takayuki Ohnishi, Tetsu Kinoshita, Toshiaki Mitsui

    FRONTIERS IN PLANT SCIENCE   9 ( 266 )   1 - 13   2018.2

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    DOI： 10.3389/fpls.2018.00266

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  • Proteomic analysis of rice golgi membranes isolated by floating through discontinuous sucrose density gradient Reviewed

    Kazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko, Toshiaki Mitsui

    Methods in Molecular Biology   1696   91 - 105   2018

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    DOI： 10.1007/978-1-4939-7411-5_6

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  • Identification and Validation of Human Missing Proteins and Peptides in Public Proteome Databases: Data Mining Strategy Reviewed

    Amr Elguoshy, Yoshitoshi Hirao, Bo Xu, Suguru Saito, Ali F. Quadery, Keiko Yamamoto, Toshiaki Mitsui, Tadashi Yamamoto

    JOURNAL OF PROTEOME RESEARCH   16 ( 12 )   4403 - 4414   2017.12

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    DOI： 10.1021/acs.jproteome.7b00423

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  • Physiological mechanism of growth-promoting effects of abscisic acid on rice mesocotyl

    Watanabe Hajime, Kamimura Kazuma, Adachi Yusuke, Itayagoshi Shigeto, Takamatsu Sou, Toshiaki Mitsui

    Abstracts of Meeting of the CSSJ   244   125 - 125   2017

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    DOI： 10.14829/jcsproc.244.0_125

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  • N-glycomic and microscopic subcellular localization analyses of NPP1, 2 and 6 strongly indicate that trans-golgi compartments participate in the golgi to plastid traffic of nucleotide pyrophosphatase/phosphodiesterases in rice Reviewed

    Kentaro Kaneko, Takeshi Takamatsu, Takuya Inomata, Kazusato Oikawa, Kimiko Itoh, Kazuko Hirose, Maho Amano, Shin-Ichiro Nishimura, Kiminori Toyooka, Ken Matsuoka, Javier Pozueta-Romero, Toshiaki Mitsui

    Plant and Cell Physiology   57 ( 8 )   1610 - 1628   2016.8

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    DOI： 10.1093/pcp/pcw089

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  • N-Glycomic and Microscopic Subcellular Localization Analyses of NPP1, 2 and 6 Strongly Indicate that trans-Golgi Compartments Participate in the Golgi to Plastid Traffic of Nucleotide Pyrophosphatase/Phosphodiesterases in Rice Reviewed

    Kentaro Kaneko, Takeshi Takamatsu, Takuya Inomata, Kazusato Oikawa, Kimiko Itoh, Kazuko Hirose, Maho Amano, Shin-Ichiro Nishimura, Kiminori Toyooka, Ken Matsuoka, Javier Pozueta-Romero, Toshiaki Mitsui

    PLANT AND CELL PHYSIOLOGY   57 ( 8 )   1610 - 1628   2016.8

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  • Proteomic and Glycomic Characterization of Rice Chalky Grains Produced Under Moderate and High-temperature Conditions in Field System Reviewed

    Kentaro Kaneko, Maiko Sasaki, Nanako Kuribayashi, Hiromu Suzuki, Yukiko Sasuga, Takeshi Shiraya, Takuya Inomata, Kimiko Itoh, Marouane Baslam, Toshiaki Mitsui

    RICE   9 ( 1 )   26   2016.5

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  • Effect of Adding Appropriate Mixture of NPK and Chicken Manure on Growth and Yield of TR-9 Paddy Variety on Beach Ridges Interspersed with Swales (BRIS) Soil

    Azwan AWANG, Mohamadu Boyie JALLOH, Pang Su KUAN, Kimiko ITOH, Toshiaki MITSUI,, Dandan ALIDIN

    新潟大学農学部研究報告   68   43 - 48   2016.2

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  • Molecular physiological aspects of chalking mechanism in rice grains under high-temperature stress Invited Reviewed

    Toshiaki Mitsui, Hiromoto Yamakawa, Tohru Kobata

    PLANT PRODUCTION SCIENCE   19 ( 1 )   22 - 29   2016

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    DOI： 10.1080/1343943X.2015.1128112

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  • Golgi-to-plastid trafficking of proteins through secretory pathway: Insights into vesicle-mediated import toward the plastids Reviewed

    Marouane Baslam, Kazusato Oikawa, Aya Kitajima-Koga, Kentaro Kaneko, Toshiaki Mitsui

    PLANT SIGNALING & BEHAVIOR   11 ( 9 )   e1221558   2016

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    DOI： 10.1080/15592324.2016.1221558

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  • Alpha-amylase expression and germination in low temperature soaking condition of rice seeds

    Itayagoshi Shigeto, Fukushima Rozan, Iwatsu Masakazu, Mitsui Toshiaki

    Abstracts of Meeting of the CSSJ   241   114 - 114   2016

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  • Golgi/plastid-type manganese superoxide dismutase involved in heat-stress tolerance during grain filling of rice Reviewed

    Takeshi Shiraya, Taiki Mori, Tatsuya Maruyama, Maiko Sasaki, Takeshi Takamatsu, Kazusato Oikawa, Kimiko Itoh, Kentaro Kaneko, Hiroaki Ichikawa, Toshiaki Mitsui

    PLANT BIOTECHNOLOGY JOURNAL   13 ( 9 )   1251 - 1263   2015.12

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    DOI： 10.1111/pbi.12314

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  • Suppressive Effects of Low Seed-Soaking Temperatures on Germination of Long-Term-Stored Rice Seeds Reviewed

    Shigeto Itayagoshi, Seiichi Mizusawa, Osamu Kawakami, Hiroshi Shibukawa, Takeshi Takamatsu, Maiko Sasaki, Kentaro Kaneko, Toshiaki Mitsui

    PLANT PRODUCTION SCIENCE   18 ( 4 )   455 - 463   2015.10

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  • Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis (vol 1, 15035, 2015) Reviewed

    Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa, Mikio Nishimura

    NATURE PLANTS   1 ( 4 )   2015.4

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  • Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis Reviewed

    Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa, Mikio Nishimura

    NATURE PLANTS   1 ( 4 )   2015.3

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  • Germination Disorder and the Improvement of Seed Pretreatment of Stored Rice Seeds (Second Report) : Effects of Initial Seed-soaking Temperature as Germination Inhibiter

    ITAYAGOSHI Shigeto, MIZUSAWA Sei-ichi, SHIBUKAWA Hiroshi, TAKAMATSU Takeshi, MITSUI Toshiaki

    The Hokuriku Crop Science   50   32 - 35   2015.3

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  • Determination of genomic location and structure of the transgenes in marker-free rice-based cholera vaccine by using whole genome resequencing approach Reviewed

    Mio Mejima, Koji Kashima, Masaharu Kuroda, Natsumi Takeyama, Shiho Kurokawa, Yoshiko Fukuyama, Hiroshi Kiyono, Kimiko Itoh, Toshiaki Mitsui, Yoshikazu Yuki

    PLANT CELL TISSUE AND ORGAN CULTURE   120 ( 1 )   35 - 48   2015.1

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  • A proteomic analysis of Nipponia nippon (ID#162) Reviewed

    Mitsuru Oyanagi, Kentaro Kaneko, Yoshinori Kaneko, Maiko Sasaki, Chizuko Nishida, Yoichi Matsuda, Toshiaki Mitsui

    ANIMAL SCIENCE JOURNAL   85 ( 8 )   814 - 832   2014.8

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  • HPLC-MS/MS Analyses Show That the Near-Starchless aps1 and pgm Leaves Accumulate Wild Type Levels of ADPglucose: Further Evidence for the Occurrence of Important ADPglucose Biosynthetic Pathway(s) Alternative to the pPGI-pPGM-AGP Pathway Reviewed

    Abdellatif Bahaji, Edurne Baroja-Fernandez, Angela Maria Sanchez-Lopez, Francisco Jose Munoz, Jun Li, Goizeder Almagro, Manuel Montero, Pablo Pujol, Regina Galarza, Kentaro Kaneko, Kazusato Oikawa, Kaede Wada, Toshiaki Mitsui, Javier Pozueta-Romero

    PLOS ONE   9 ( 8 )   e104997   2014.8

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  • Stress enhances the gene expression and enzyme activity of phenylalanine ammonia-lyase and the endogenous content of salicylic acid to induce flowering in pharbitis

    Wada, K.C., Mizuuchi, K., Koshio, A., Kaneko, K., Mitsui, T., Takeno, K.

    Journal of Plant Physiology   171 ( 11 )   895 - 902   2014.7

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  • Stress enhances the gene expression and enzyme activity of phenylalanine ammonia-lyase and the endogenous content of salicylic acid to induce flowering in pharbitis Reviewed

    Kaede C. Wada, Kaori Mizuuchi, Aya Koshio, Kentaro Kaneko, Toshiaki Mitsui, Kiyotoshi Takeno

    JOURNAL OF PLANT PHYSIOLOGY   171 ( 11 )   895 - 902   2014.7

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  • Crystal structure of alpha-amylase from Oryza sativa: molecular insights into enzyme activity and thermostability Reviewed

    Akihito Ochiai, Hiroshi Sugai, Kazuki Harada, Seiya Tanaka, Yohei Ishiyama, Kosuke Ito, Takaaki Tanaka, Toshio Uchiumi, Masayuki Taniguchi, Toshiaki Mitsui

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   78 ( 6 )   989 - 997   2014.6

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  • alpha-Amylase is a potential growth inhibitor of Porphyromonas gingivalis, a periodontal pathogenic bacterium Reviewed

    A. Ochiai, K. Harada, K. Hashimoto, K. Shibata, Y. Ishiyama, T. Mitsui, T. Tanaka, M. Taniguchi

    JOURNAL OF PERIODONTAL RESEARCH   49 ( 1 )   62 - 68   2014.2

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  • Nucleotide Pyrophosphatase/Phosphodiesterase 1 Exerts a Negative Effect on Starch Accumulation and Growth in Rice Seedlings under High Temperature and CO2 Concentration Conditions Reviewed

    Kentaro Kaneko, Takuya Inomata, Takahiro Masui, Tsutomu Koshu, Yukiho Umezawa, Kimiko Itoh, Javier Pozueta-Romero, Toshiaki Mitsui

    PLANT AND CELL PHYSIOLOGY   55 ( 2 )   320 - 332   2014.2

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  • Nucleotide pyrophosphatase/phosphodiesterase 1 exerts a negative effect on starch accumulation and growth in rice seedlings under high temperature and CO2 concentration conditions

    Kentaro Kaneko, Takuya Inomata, Takahiro Masui, Tsutomu Koshu, Yukiho Umezawa, Kimiko Itoh, Javier Pozueta-Romero, Toshiaki Mitsui

    Plant and Cell Physiology   55 ( 2 )   320 - 332   2014.2

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  • Quantitative proteomic analysis of intact plastids Reviewed

    Takeshi Shiraya, Kentaro Kaneko, Toshiaki Mitsui

    Methods in Molecular Biology   1072   469 - 480   2014

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    DOI： 10.1007/978-1-62703-631-3_32

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  • Rapid and high-throughput N-glycomic analysis of plant glycoproteins Reviewed

    Kentaro Kaneko, Takeshi Shiraya, Toshiaki Mitsui, Shin-Ichiro Nishimura

    Methods in Molecular Biology   1072   645 - 653   2014

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    DOI： 10.1007/978-1-62703-631-3_44

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  • A proteomic analysis of Nipponia nippon (ID#162)

    Mitsuru Oyanagi, Kentaro Kaneko, Yoshinori Kaneko, Maiko Sasaki, Chizuko Nishida, Yoichi Matsuda, Toshiaki Mitsui

    Animal Science Journal   85 ( 8 )   814 - 832   2014

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    DOI： 10.1111/asj.12214

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  • Arabidopsis glycerol-3-phosphate permease 4 is localized in the plastids and involved in the accumulation of seed oil Reviewed

    Hiromitsu Kawai, Toshiki Ishikawa, Toshiaki Mitsui, Shin Kore-eda, Maki Kawai-Yamada, Jun-ichi Ohnishi

    PLANT BIOTECHNOLOGY   31 ( 2 )   159 - U80   2014

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    DOI： 10.5511/plantbiotechnology.14.0222a

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  • Identification of oxidatively modified proteins in salt-stressed arabidopsis: A carbonyl-targeted proteomics approach Reviewed

    Jun'ichi Mano, Mitsuaki Nagata, Shoutarou Okamura, Takeshi Shiraya, Toshiaki Mitsui

    Plant and Cell Physiology   55 ( 7 )   1233 - 1244   2014

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    DOI： 10.1093/pcp/pcu072

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  • Effects of Water Temperature during Soaking Period of Rice Seeds Stored for a Long Term on Sucrose Synthase Expression during Germination

    ITAYAGOSHI Shigeto, KANEKO Kentaro, SASAKI Maiko, HIROSE Tatsuro, MITSUI Toshiaki

    82   136 - 137   2013.9

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  • Characteristics of Opaque and Translucent Parts of High Temperature Stressed Grains of Rice Reviewed

    K. Tsutsui, K. Kaneko, I. Hanashiro, K. Nishinari, T. Mitsui

    Journal of Applied Glycoscience   60 ( 1 )   61 - 67   2013.4

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  • Proteonnics of rice grain under high temperature stress

    Toshiaki Mitsui, Takeshi Shiraya, Kentaro Kaneko, Kaede Wada

    FRONTIERS IN PLANT SCIENCE   4 ( 36 )   2013.3

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    DOI： 10.3389/fpls.2013.00036

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  • Recent Progress in α-Amylase Biosynthesis Reviewed

    Takashi Akazawa, Toshiaki Mitsui, Makoto Hayashi

    The Biochemistry of Plants: A Comprehensive Treatise   14   465 - 492   2012.12

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    DOI： 10.1016/B978-0-08-092615-5.50017-5

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  • Suppression of α-amylase genes improves quality of rice grain ripened under high temperature. Reviewed

    M. Hakata, M. Kuroda, T. Miyashita, T. Yamaguchi, M. Kojima, H. Sakakibara, T. Mitsui, H. Yamakawa

    Plant Biotechnology Journal   10 ( 9 )   1110 - 1117   2012.10

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  • A null mutation of ROS1a for DNA demethylation in rice is not transmittable to progeny Reviewed

    Akemi Ono, Katsushi Yamaguchi, Sachiko Fukada-Tanaka, Rie Terada, Toshiaki Mitsui, Shigeru Iida

    PLANT JOURNAL   71 ( 4 )   564 - 574   2012.8

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    DOI： 10.1111/j.1365-313X.2012.05009.x

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  • A 50-Kilodalton Cry2A Peptide Is Lethal to Bombyx mori and Lymantria dispar

    Masataka Ohsawa, Miki Tanaka, Kenta Moriyama, Mitsuaki Shimazu, Shin-ichiro Asano, Kazuhisa Miyamoto, Kohsuke Haginoya, Toshiaki Mitsui, Tomoaki Kouya, Masayuki Taniguchi, Hidetaka Hori

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   78 ( 13 )   4755 - 4757   2012.7

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    DOI： 10.1128/AEM.07123-11

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  • Rice kinesin O12 is identical to kinesin OsKCH1 Reviewed

    Umezu, N., Umeki, N., Mitsui, T., Kondo, K., Maruta, S.

    Journal of Biochemistry   152 ( 2 )   207 - 208   2012.5

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    DOI： 10.1093/jb/mvs076

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  • Involvement of Endomembrane-type Superoxide Dismutase in High Temperature Stress Tolerance during Grain Filling of Rice(proceedings) Invited Reviewed

    T. Mitsui, T. Shiraya, T. Mori, K. Kaneko

    Frontiers in Agriculture Proteome Reseach   56 - 61   2012.3

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  • Salicylic acid is involved in the regulation of starvation stress-induced flowering in Lemna paucicostata Reviewed

    Shimakawa, A., Shiraya, T., Ishizuka, Y., Wada, K.C., Mitsui, T., Takeno, K.

    Journal of Plant Physiology   169 ( 10 )   987 - 991   2012.2

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    DOI： 10.1016/j.jplph.2012.02.009

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  • A chemically inducible gene expression system and its application to inducible gene suppression in rice Reviewed

    Tatsuro Hirose, Rie Mizutani, Toshiaki Mitsui, Tomio Terao

    Plant Production Science   15 ( 2 )   73 - 78   2012

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    DOI： 10.1626/pps.15.73

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  • Elution behavior analysis of starch degrading enzymes during rice cooking with specific antibodies Reviewed

    Mika Tsuyukubo, Tetsuya Ookura, Shinya Tsukui, Toshiaki Mitsui, Midori Kasai

    Food Science and Technology Research   18 ( 5 )   659 - 666   2012

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    DOI： 10.3136/fstr.18.659

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  • Analysis of quantitative proteomics in rice <i>npp1</i>under the high temperature high CO<sub>2</sub> condition

    Kaneko Kentaro, Shiraya Takeshi, Inomata Takuya, Koshu Tsutomu, Masui Takahiro, Kitajima-Koga aya, Mitsui Toshiaki

    Abstracts for Annual Meeting of Japanese Proteomics Society   2012   152 - 152   2012

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    DOI： 10.14889/jhupo.2012.0.152.0

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  • Proteomic Analysis of White Immature Grains of Rice Caused by High Temperature Stress during Grain Filling

    Mitsui Toshiaki, Sasaki Maiko, Maruyama Tatsuya, Shiraya Takeshi, Kaneko Kentaro

    Abstracts for Annual Meeting of Japanese Proteomics Society   2012   61 - 61   2012

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    DOI： 10.14889/jhupo.2012.0.61.0

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  • Quantitative proteomic analysis of white immature rice grain caused by high temperature stress

    Sasaki Maiko, Maruyama Tatsuya, Shiraya Takeshi, Kaneko Kentaro, Mitsui Toshiaki

    Abstracts for Annual Meeting of Japanese Proteomics Society   2012   170 - 170   2012

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  • Proteomic Analysis of Rice Golgi Apparatus

    Ishiyama Ryuichi, Ito Namiko, Nakayama Yuki, Mitsui Toshiaki

    Abstracts for Annual Meeting of Japanese Proteomics Society   2012   118 - 118   2012

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    DOI： 10.14889/jhupo.2012.0.118.0

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  • Characterization of the Nuclear- and Plastid-Encoded secA-Homologous Genes in the Unicellular Red Alga Cyanidioschyzon merolae Reviewed

    Yosuke Koyama, Koji Takimoto, Asuka Kojima, Kei Asai, Satoshi Matsuoka, Toshiaki Mitsui, Kouji Matsumoto, Hiroshi Hara, Niji Ohta

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   75 ( 10 )   2073 - 2078   2011.10

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    DOI： 10.1271/bbb.110338

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  • Midgut juice of Plutella xylostella highly resistant to Bacillus thuringiensis Cry1Ac contains a three times larger amount of glucosinolate sulfatase which binds to Cry1Ac compared to that of susceptible strain Reviewed

    Takanori Yamazaki, Toshiki Ishikawa, Ganesh N. Pandian, Keiichi Okazaki, Kohsuke Haginoya, Yuka Tachikawa, Toshiaki Mitsui, Kazuhisa Miyamoto, Chanan Angusthanasombat, Hidetaka Hori

    PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY   101 ( 2 )   125 - 131   2011.10

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    DOI： 10.1016/j.pestbp.2011.09.001

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  • Biochemical characterization of the novel rice kinesin K23 and its kinetic study using fluorescence resonance energy transfer between an intrinsic tryptophan residue and a fluorescent ATP analogue Reviewed

    Nozomi Umezu, Nobue Hanzawa, Masafumi D. Yamada, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta

    JOURNAL OF BIOCHEMISTRY   149 ( 5 )   539 - 550   2011.5

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    DOI： 10.1093/jb/mvr012

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  • Ethylene production during clubroot development in turnip

    T.Ishikawa, K. Okazaki, K. Itoh, T. Mitsui, H. Hori

    新潟大学農学部研究報告   63 ( 2 )   83 - 87   2011.3

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  • Changes in rice Golgi apparatus in response to sugar starvation

    T.Mitsui, T. Asakura, K. Takayama, Y. Nakayama, R. Ishiyama, S. Hirose, H. Katamine, M. Aoyama, K. Kaneko, K. Itoh, H. Hori

    新潟大学農学部研究報告   63 ( 2 )   63 - 68   2011.3

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    Other Link： http://hdl.handle.net/10191/18614

  • Bacillus thuringiensis cytotoxin, Cyt2Aa killed Myzus persicae (Sulzer) (Hemiptera: Aphididae)

    N. Koyama, Y. Sano, M. Tanaka, K. Haginoya, B. Promodonkoy, C. Angsuthanasombat, T. Mitsui, T. Kohya, M. Taniguchi, K. Okazaki, H. Hori

    新潟大学農学部研究報告   63 ( 2 )   77 - 81   2011.3

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  • Interaction between Bacillus thuringiensis Cyt2Aa toxin and phosphatidylcholine-liposome

    N. Koyama, K. Haginoya, M. Tanaka, B. Promodonkoy, C. Angsuthanasobat, T. Mitsui, M. Taniguchi, T. Kohya, K. Itoh, H. Hori

    新潟大学農学部研究報告   63 ( 2 )   69 - 76   2011.3

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  • Characterization of the Ry3378c Gene Product, a New Diterpene Synthase for Producing Tuberculosinol and (13R, S)-Isotuberculosinol (Nosyberkol), from the Mycobacterium tuberculosis H37Rv Genome Reviewed

    Chiaki Nakano, Takahiro Ootsuka, Kazutoshi Takayama, Toshiaki Mitsui, Tsutomu Sato, Tsutomu Hoshino

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   75 ( 1 )   75 - 81   2011.1

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    DOI： 10.1271/bbb.100570

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  • Characterization of a novel rice kinesin O12 with a calponin homology domain Reviewed

    Nozomi Umezu, Nobuhisa Umeki, Toshiaki Mitsui, Kazunori Kondo, Shinsaku Maruta

    JOURNAL OF BIOCHEMISTRY   149 ( 1 )   91 - 101   2011.1

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    DOI： 10.1093/jb/mvq122

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  • Differential localizations and functions of rice nucleotide pyrophosphatase/phosphodiesterase isozymes 1 and 3 Reviewed

    Kentaro Kaneko, Chie Yamada, Ai Yanagida, Tsutomu Koshu, Yukiho Umezawa, Kimiko Itoh, Hidetaka Hori, Toshiaki Mitsui

    PLANT BIOTECHNOLOGY   28 ( 1 )   69 - 76   2011

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    DOI： 10.5511/plantbiotechnology.10.1228a

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  • Search and functional analysis of genes involved in high temperature tolerance during grain filling of rice

    Shiraya Takeshi, Mori Taiki, Okubo Ena, Maruyama Tatsuya, Kaneko Kentaro, ??? ???

    Abstracts for Annual Meeting of Japanese Proteomics Society   2011   188 - 188   2011

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    DOI： 10.14889/jhupo.2011.0.188.0

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  • Quantitative proteome analysis of rice grain filling under high temperature stress

    Shiraya Takeshi, Mori Taiki, Okubo Ena, Maruyama Tatsuya, Kaneko Kentaro, ??? ???

    Abstracts for Annual Meeting of Japanese Proteomics Society   2011   214 - 214   2011

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    DOI： 10.14889/jhupo.2011.0.214.0

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  • Physico-chemical Properties of White-core Rice Kernels

    Tsutsui Kazumi, Hirokami Rina, Kaneko Kentaro, Nishinari Katsuyoshi, Mitsui Toshiaki

    Journal of Applied Glycoscience Supplement   2011   27 - 27   2011

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    DOI： 10.11541/jsag.2011.0.27.0

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  • 1M1436 Kinetic characterization of the Rice Plant Kinesins using Fluorescent-ATP Analogue(Molecular motor 2,The 49th Annual Meeting of the Biophysical Society of Japan)

    Umezu Nozomi, Kondo Kazunori, Mitsui Toshiaki, Maruta Shinsaku

    Seibutsu Butsuri   51   S64   2011

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    DOI： 10.2142/biophys.51.S64_2

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  • Formation of macromolecule complex with bacillus thuringiensis Cry1A toxins and chlorophyllide binding 252-kDa lipocalin-like protein locating on bombyx mori midgut membrane Reviewed

    Pandian, G.N., Ishikawa, T., Vaijayanthi, T., Hossain, D.M., Yamamoto, S., Nishiumi, T., Angsuthanasombat, C., Haginoya, K., Mitsui, T., Hori, H.

    Journal of Membrane Biology   237 ( 2-3 )   125 - 136   2010.11

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    DOI： 10.1007/s00232-010-9314-x

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  • Proteomic analysis of Theobroma cacao pod husk Reviewed

    A. Awang, R. Karim, T. Mitsui

    Journal of Applied Glycoscience   57 ( 4 )   245 - 264   2010.11

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  • Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K16 Reviewed

    K. Tanaka, N. Umeki, T. Mitsui, Z. Fujimoto, S. Maruta

    Biochemical and Biological Research Communications   401 ( 2 )   251 - 256   2010.9

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  • イネゴルジ体プロテオーム : N－アセチルグルコサミニルトランスフェラーゼ－I様タンパク質の同定と機能解析

    63 ( 1 )   19 - 27   2010.8

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  • Isoform-specific localization of Brassica rapa nitrilases in root infected with Plasmodiophora brassicae revealed using in situ hybridization probes improved with locked nucleic acids Reviewed

    Toshiki Ishikawa, Keiichi Okazaki, Tomohiko Nagaoka, Kimiko Itoh, Toshiaki Mitsui, Hidetaka Hori

    Journal of Plant Growth Regulation   29 ( 2 )   210 - 222   2010.6

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    DOI： 10.1007/s00344-009-9131-6

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  • Synthesis of high quality probe using locked nucleic acid (LNA) and exploitation for ISH

    T. Ishikawa, K. Okazaki, K. Itoh, T. Mitsui, H. Hori

    Bulletin of the Faculty of Agriculture, Niigata University   63 ( 1 )   35 - 39   2010.3

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  • Rice Golgi proteome: Identification and characterization of N-acetylglucosaminyltransferase-I-like protein

    T.Mitsui, T. Asakura, Y. Nakayama, K. Takayama, S. Hirose, H. Katamine, M. Aoyama, A. Karahashi, K. Kaneko, R. Ishiyama, K. Itoh, H. Hori, H. Kajiura, K. Fujiyama

    新潟大学農学部研究報告   63 ( 1 )   19 - 27   2010.3

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  • Studies on plastid targeting signal of rice α-amylase I-1

    Mizutani Rie, Okada Hisao, Hamada Yuki, Okubo Ena, Itoh Taeko, Itoh Kimiko, Mitsui Tosiaki

    Journal of Applied Glycoscience Supplement   2010   33 - 33   2010

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  • Study for physicochemical property of milky white rice kernel

    Tsutsui Kazumi, Harada Ayumi, Kaneko Kentaro, Nishinari Katsuyoshi, Mitsui Toshiaki

    Journal of Applied Glycoscience Supplement   2010   47 - 47   2010

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  • 2P181 Kinetic studies on the novel rice kinesin K23 utilizing FRET between the intrinsic tryptophan residue and fluorescent ATP analogue(The 48th Annual Meeting of the Biophysical Society of Japan)

    Umezu Nozomi, Hanzawa Nobue, Kondo Kazunori, Mitsui Toshiaki, Maruta Shinsaku

    Seibutsu Butsuri   50 ( 2 )   S114   2010

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    DOI： 10.2142/biophys.50.S114_3

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  • Glycoproteomic analysis of chloroplasts isolated from rice mature leaves

    Y. Hamada, A. Kitajima, H. Okada, E. Okubo, A. Awang, K. Kaneko, K. Takayama, T. Mitsui

    新潟大学農学部研究報告   62 ( 1 )   25 - 30   2009.12

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  • The rice α-amylase glycoprotein is targeted from the Golgi apparatus through the secretory pathway to the plastids. Reviewed

    A. Kitajima, S. Asatsuma, H. Okada, Y. Hamada, K. Kaneko, Y. Nanjo, Y. Kawagoe, K. Toyooka, K. Matsuoka, A. Nakano, T. Mitsui

    The Plant Cell   21 ( 9 )   2844 - 2858   2009.9

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  • Carbon and nitrogen transport during grain filling in rice under high-temperature conditions Reviewed

    S. Ito, T. Hara, Y. Kawakami, T. Watanabe, K. Thiraporn, N. Otake, K. Sueyoshi, T. Mitsui, T. Fukuyama, Y. Takahashi, T. Sato, A. Sato, T. Ohyama

    Journal of Agronomy and Crop Science   195 ( 5 )   368 - 376   2009.4

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  • A Mobile Secretory Vesicle Cluster Involved in Mass Transport from the Golgi to the Plant Cell Exterior Reviewed

    Kiminori Toyooka, Yumi Goto, Satoru Asatsuma, Masato Koizumi, Toshiaki Mitsui, Ken Matsuoka

    PLANT CELL   21 ( 4 )   1212 - 1229   2009.4

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    DOI： 10.1105/tpc.108.058933

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  • 2P-111 Interaction of the novel rice kineisn O12 that has the calponin homology domain with actin(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Umezu Nozomi, Kondo Kazunori, Mitsui Toshiaki, Maruta Shinsaku

    Seibutsu Butsuri   49   S123   2009

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    DOI： 10.2142/biophys.49.S123_4

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  • 2P-112 Characterization of the novel rice kinesin K23(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Hanzawa Nobue, Umezu Nozomi, Kondo Kazunori, Mitsui Toshiaki, Maruta Shinsaku

    Seibutsu Butsuri   49   S123 - S124   2009

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    DOI： 10.2142/biophys.49.S123_5

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  • Plastidial localization of a potato "Nudix" hydrolase of ADPglucose linked to starch biosynthesis Reviewed

    F.J. Munoz, E. Baroja-Fernandez, M. Ovecka, J. Li, T. Mitsui, M.T. Sesma, M. Montero, A. Bahaji, I. Ezquer, J. Pozeta-Romero

    Plant and Cell Physiology   49 ( 11 )   1734 - 1746   2008.4

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  • Functional Analysis of Rice Nucleotide Pyrophosphatase/Phosphodiesterase

    Yanagida Ai, Kaneko Kentaro, Yamada Chie, Itoh Kimiko, Mitsui Toshiaki

    Journal of Applied Glycoscience Supplement   2008   87 - 87   2008

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    DOI： 10.11541/jsag.2008.0.87.0

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  • 2P-170 Kinetic characterization of the novel rice kineisn L05 and D04 using fluorescent ATP analogue(The 46th Annual Meeting of the Biophysical Society of Japan)

    Umezu Nozomi, Kubo Yuko, Kondo Kazunori, Mitsui Toshiaki, Maruta Shinsaku

    Seibutsu Butsuri   48   S101   2008

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    DOI： 10.2142/biophys.48.S101_4

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  • alpha-amylase production is induced by sulfuric acid in rice aleurone cells Reviewed

    Shin-ichiro Mitsunaga, Midori Kobayashi, Satoe Fukui, Kayoko Fukuoka, Osamu Kawakami, Junji Yamaguchi, Masahiro Ohshima, Toshiaki Mitsui

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   45 ( 12 )   922 - 925   2007.12

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  • Histochemical analysis of Bacillus thuringiensis Cry1A toxin binding to midgut epithelial cells of Bombyx mori Reviewed

    D. M. Hossain, T. Hayakawa, Y. Shitomi, K. Itoh, T. Mitsui, R. Sato, H. Hori

    Pesticide Biochemistry and Physiology   87 ( 1 )   30 - 38   2007.12

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  • Loadingof Fura-2 into liquid organ cultured adventitious root of turnip（Brassica rapa L.) Resistant to clubroot pathogen Plasmodiophora brassicae and determination of [Ca2+] cyt.

    H. Takahashi, T. Ishikawa, T. Hayakawa, K. Itoh, T. Mitsui, H. Hori

    新潟大学農学部研究報告   60 ( 1 )   61 - 66   2007.6

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  • Evaluation of roles of amidase which converts indole-3-acetamide to indole-3-acetic acid, in formation of clubroot in turnip.

    T. Ishikawa, H Kuroda, K. Okazaki, K. Itoh, T.Mitsui, H. Hori

    新潟大学農学部研究報告   60 ( 1 )   53 - 60   2007.6

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  • Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development

    Ishikawa, T., Okazaki, K., Kuroda, H., Itoh, K., Mitsui, T., Hori, H.

    Molecular Plant Pathology   8 ( 5 )   623 - 637   2007.2

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    DOI： 10.1111/j.1364-3703.2007.00414.x

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  • Proteomic characterization of tissue expansion of rice scutellum stimulated by abscisic acid Reviewed

    Tsuyoshi Asakura, Shota Hirose, Satoru Asatsuma, Yohei Nanjo, Tetsuyo Nakaizumi, Kimiko Itoh, Hidetaka Hori, Setsuko Komatsu, Toshiaki Mitsui

    Bioscience, Biotechnology and Biochemistry   71 ( 5 )   1260 - 1268   2007

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    DOI： 10.1271/bbb.60675

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  • 2P154 Biochemical analysis of the Rice Kinesin 012 using Fluorescent-ATP Analogue(Molecular motors,Poster Presentations)

    Umezu Nozomi, Umeki Nobuhisa, Kondo Kazunori, Mitsui Toshiaki, Maruta Shinsaku

    Seibutsu Butsuri   47   S151   2007

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    DOI： 10.2142/biophys.47.S151_3

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  • Conformational change of the loop l5 in rice kinesin motor domain induced by nucleotide binding Reviewed

    Umeki, N., Mitsui, T., Kondo, K., Maruta, S.

    Journal of Biochemistry   139 ( 5 )   857 - 864   2006.12

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    DOI： 10.1093/jb/mvj102

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  • Plasmodiophora brassicae-induced cell death and medium alkalization in clubroot-resistant cultured roots of Brassica rapa Reviewed

    Takahashi, H., Ishikawa, T., Kaido, M., Takita, K., Hayakawa, T., Okazaki, K., Itoh, K., Mitsui, T., Hori, H.

    Journal of Phytopathology   154 ( 3 )   156 - 162   2006.12

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    DOI： 10.1111/j.1439-0434.2006.01076.x

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  • alpha-amylase affects starch accumulation in rice grain Reviewed

    S. Asatsuma, C. Sawada, A. Kitajima, T. Asakura, T. Mitsui

    J. Appl. Glycosci.   53 ( 3 )   187 - 192   2006.12

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  • Preparation and characterization of a novel rice plant-specific kinesin Reviewed

    Umeki, N., Mitsui, T., Umezu, N., Kondo, K., Maruta, S.

    Journal of Biochemistry   139 ( 4 )   645 - 654   2006.12

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    DOI： 10.1093/jb/mvj074

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  • Intermolecular cross-linking of a novel rice kinesin K16 motor domain with a photoreactive ATP derivative Reviewed

    Umeki, N., Mitsui, T., Koike, Y., Maruta, S.

    Journal of Biochemistry   139 ( 5 )   831 - 836   2006.12

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    DOI： 10.1093/jb/mvj098

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  • Rice plastidial N-glycosylated nucleotide pyrophosphatase/phosphodiesterase is transported from the ER-golgi to the chloroplast through the secretory pathway Reviewed

    Yohei Nanjo, Hiromasa Oka, Noriko Ikarashi, Kentaro Kaneko, Aya Kitajima, Toshiaki Mitsui, Francisco José Muñoz, Milagros Rodríguez-López, Edurne Baroja-Fernandez, Javier Pozueta-Romero

    Plant Cell   18 ( 10 )   2582 - 2592   2006.10

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    DOI： 10.1105/tpc.105.039891

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  • Bacillus thuringiensis Cry1Ab, but not Cry1Aa or Cry1Ac, disrupts liposomes Reviewed

    Taisuke Kato, Masahiro Higuchi, Ryo Endo, Takeshi Maruyama, Kousuke Haginoya, Yasuyuki Shitomi, Tohru Hayakawa, Toshiaki Mitsui, Ryoichi Sato, Hidetaka Hori

    Pesticide Biochemistry and Physiology   84 ( 1 )   1 - 9   2006.1

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    DOI： 10.1016/j.pestbp.2005.02.001

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  • Isolation and characterization of GAMYB mutant rice.

    Y. Tanaka, K. Nakano, T. Mitsui, H. Hori, K. Itoh

    新潟大学農学部研究報告   58 ( 2 )   103 - 108   2006.1

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  • Isolation and proteomic analysis of rice Golgi membranes: Cis-Golgi membranes labeled with GFP-SYP31 Reviewed

    Tsuyoshi Asakura, Shota Hirose, Hiroki Katamine, Aya Kitajima, Hidetaka Hori, Masa H. Sato, Masayuki Fujiwara, Ko Shimamoto, Toshiaki Mitsui

    Plant Biotechnology   23 ( 5 )   475 - 485   2006

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    DOI： 10.5511/plantbiotechnology.23.475

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  • 2P219 Interaction of a novel rice kinesin O12 with fluorescent ATP analogue(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Umezu Nozomi, Kubo Yuko, Umeki Nobuhisa, Kazunori Kondo, Mitsui Toshiaki, Maruta Shinsaku

    Seibutsu Butsuri   46 ( 2 )   S350   2006

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    DOI： 10.2142/biophys.46.S350_3

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  • 2P216 Nucleotide dependent intermolecular cross-linking of rice kinesin by photo reactive ATP analogue(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Koike Yutaka, Umeki Nobuhisa, Mitsui Toshiaki, Sugimoto Yasunobu, Wakabayashi Katsuzo, Maruta Shinsaku

    Seibutsu Butsuri   46 ( 2 )   S349   2006

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    DOI： 10.2142/biophys.46.S349_4

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  • Localization of a novel 252-kDa plasma membrane protein that binds Cry1A toxins in the midgut epothelia of Bombyx mori. Reviewed

    D.M. Hossain, Y. Shitomi, Y. Nanjo, D. Takano, T. Nishiumi, T. Hayakawa, T. Mitsui, R. Sato, H. Hori

    Appl. Entomol. Zool.   40 ( 1 )   125 - 135   2005.12

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    DOI： 10.1303/aez.2005.125

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  • Measurement of the concentration of bioactive gibberellin in germinating rice seed using the α-amylase induction from aleurone cells. Reviewed

    Shin-ichiro Mitsunaga, Osamu Kawakami, Junji Yamaguchi, Toshiaki Mitsui

    J. Appl. Glycosci.   52   399 - 402   2005.12

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  • Involvement of α-amylase I-1 in starch degradation in rice chloroplasts Reviewed

    Asatsuma, S., Sawada, C., Itoh, K., Okito, M., Kitajima, A., Mitsui, T.

    Plant and Cell Physiology   46 ( 6 )   858 - 869   2005.12

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    DOI： 10.1093/pcp/pci091

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  • Preparation of stable liposomes using sucrose density gradient centrifugation and their interaction with insecticidal Cry1A toxins of Bacillus thuringiensis

    K. Haginoya, T. Kato, M. Higuchi, Y. Shitomi, T. Asakura, T. Hayakawa, T. Mitsui, H. Hori

    新潟大学農学部研究報告   57 ( 2 )   115 - 120   2005.1

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  • Characterization of a novel plasma membrane protein, expressed in the midgut epithelia of Bombyx mori, that binds to Cry1A toxins Reviewed

    Hossain, D.M., Shitomi, Y., Moriyama, K., Higuchi, M., Hayakawa, T., Mitsui, T., Sato, R., Hori, H.

    Applied and Environmental Microbiology   70 ( 8 )   4604 - 4612   2004.12

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    DOI： 10.1128/AEM.70.8.4604-4612.2004

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  • Proteomic identification of α-amylase isoforms encoded by RAmy3B/3C from germinating rice seeds Reviewed

    Nanjo, Y., Asatsuma, S., Itoh, K., Hori, H., Mitsui, T.

    Bioscience, Biotechnology and Biochemistry   68 ( 1 )   112 - 118   2004.12

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    DOI： 10.1271/bbb.68.112

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  • Two rice GRAS family genes responsive to N-acetylchitooligosaccharide elicitor are induced by phytoactive gibberellins: Evidence for cross-talk between elicitor and gibberellin signaling in rice cells Reviewed

    Day, R.B., Tanabe, S., Koshioka, M., Mitsui, T., Itoh, H., Ueguchi-Tanaka, M., Matsuoka, M., Kaku, H., Shibuya, N., Minami, E.

    Plant Molecular Biology   54 ( 2 )   261 - 272   2004.12

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    DOI： 10.1023/B:PLAN.0000028792.72343.ee

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  • Proteomics of the rice cell: systematic identification of the protein populations in subcellular compartments. Reviewed

    N. Tanaka, M. Fujita, H. Handa, S. Murayama, M. Uemura, Y. Kawamura, T. Mitsui, S. Mikami, Y. Tozawa, T. Yoshianga, S. Komatsu

    Mol. Genet. Gen.   271 ( 5 )   566 - 576   2004.12

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  • Incorporation of an azobenzene derivative into the energy transducing site of skeletal muscle myosin results in photo-induced conformational changes Reviewed

    N Umeki, T Yoshizawa, Y Sugimoto, T Mitsui, K Wakabayashi, S Maruta

    JOURNAL OF BIOCHEMISTRY   136 ( 6 )   839 - 846   2004.12

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    DOI： 10.1093/jb/mvh194

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  • Posttranscriptional regulation of alpha-amylase II-4 expression by gibberellin in germinating rice seeds Reviewed

    Y Nanjo, S Asatsuma, K Itoh, H Hori, T Mitsui, Y Fujisawa

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   42 ( 6 )   477 - 484   2004.6

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    DOI： 10.1016/j.plaphy.2004.04.005

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  • Posttranscriptional regulation of a-amylase expression by giberellin in germinating rice seeds

    Yohei Nanjo, Satoru Asatsuma, Kimiko Itoh, Hidetaka Hori, Toshiaki Mitsui

    Plant Physiol. Biochem   68 ( 1 )   112 - 118   2004.3

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  • Introduction of Wx transgene into rice wx mutants leads to both high- and low-amylose rice. Reviewed

    K. Itoh, H. Ozaki, K. Okada, H. Hori, Y. Takeda, T. Mitsui

    Plant Cell Physiol.   44 ( 5 )   473 - 480   2003.12

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  • Activity of phosphatidylinositol/UDP-GlcNAc: GlcNAc transferase in midgut tissue of Bombyx mori

    D Takano, T Kishimoto, T Hayakawa, T Mitsui, H Hori

    Bulletin of the Faculty of Agriculture-Niigata University (Japan)   55 ( 2 )   123 - 132   2003.3

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  • Expression and glycosylation of glutelin in suspension-cultured cells of rice (Oryza sativa L.)

    S Kimura, T Mitsui, H Hori

    Bulletin of the Faculty of Agriculture-Niigata University (Japan)   55 ( 2 )   143 - 150   2003.3

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  • Differential extraction of the proteins localizing on cell membranes of midgut from Bombyx mori with triton X-114

    D Takano, T Kishimoto, T Hayakawa, M Higuchi, Y Shitomi, T Mitsui, H Hori

    Bulletin of the Faculty of Agriculture-Niigata University (Japan)   55 ( 2 )   133 - 142   2003.3

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  • Phosphate Modulates Ca2+ Uptake and ± Amylase Secretion in Suspension-Cultured Cells of Rice Reviewed

    Satoru Asatsuma, Yohei Nanjo, Mohammad A. Kashem, Kimiko Itoh, Hidetaka Hori, Masahiro Ohshima, Toshiaki Mitsui

    Plant Biotechnology   20 ( 3 )   187 - 194   2003

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    DOI： 10.5511/plantbiotechnology.20.187

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  • Increase in Indole 3-acetic Acid (IAA) level and Nitrilase Activity in Turnips Induced by Plasmodiophora brassicae Infection Reviewed

    Tsutomu Ugajin, Kazuo Takita, Hideyuki Takahashi, Toshiaki Mitsui, Tohru Hayakawa, Hidetaka Hori, Satoko Muraoka, Takctoshi Tada, Takuji Ohyama

    Plant Biotechnology   20 ( 3 )   215 - 220   2003

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    DOI： 10.5511/plantbiotechnology.20.215

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  • Ca<sup>2+</sup> is required by clubroot resistant turnip cells for transient increases in PAL activity that follow inoculation with Plasmodiophora brassicae Reviewed

    Takahashi, H., Takita, K., Kishimoto, T., Mitsui, T., Hori, H.

    Journal of Phytopathology   150 ( 10 )   529 - 535   2002.12

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    DOI： 10.1046/j.1439-0434.2002.00797.x

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  • A Novel ADP-glucose pyrophosphatase from rice seedlings(proceedings) Reviewed

    Y. Nanjo, S. Kurokawa, T. Mitsui, J. Pozueta-Romero

    Proceedings of 1st Spanish Congress on Physiology, Biochemistry and Molecular Biology of Carbohydrates   87 - 88   2002.12

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  • Structure and function of the Golgi complex in rice [Oryza sativa] cells: Ultrastructure of Golgi complex and localization of PNA [Peanut lectin]-recognized glycoproteins

    S. Kimura, T. Hayakawa, I. Igaue, S. Mikami, H. Hori, T. Mitsui

    Bulletin of the Faculty of Agriculture-Niigata University (Japan)   54 ( 2 )   133 - 149   2002.3

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  • Posttranscriptional regulation of alpha-amylase expression by gibberellin in germinating rice seeds Reviewed

    Y Nanjo, S Asatsuma, S Mikami, H Hori, K Itoh, T Mitsui

    PLANT AND CELL PHYSIOLOGY   43 ( 6 )   S133 - S133   2002

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  • Technical improvement to 2D-page of rice organelle membrane proteins Reviewed

    Satoshi Mikami, Tadashi Kishimoto, Hidetaka Hori, Toshiaki Mitsui

    Bioscience, Biotechnology and Biochemistry   66 ( 5 )   1170 - 1173   2002

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    DOI： 10.1271/bbb.66.1170

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  • Comparative study on capability of binding of BBMV proteins from Plutella xylostella which were highly resistant and susceptible to Cry1Ac using Bacillus thuringiensis Cry1A toxins

    Higuchi Masahiro, Maruyama Takeshi, Endo Ryo, Shitomi Yasuyuki, Hayakawa Tohru, Mitsui Toshiaki, Sato Ryoichi, Hori Hidetaka

    Abstracts of the Annual Meeting, The Japanese Society of Sericultural Science   jsss72   66 - 66   2002

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    CHAPS-soluble proteins from BBMV of Plutella xylostella which were highly resistant and susceptible to Cry1Ac of Bacillus thuringiensis were used to determine the capability of binding with Cry1A toxins. Both ligand and western blot analyses with the proteins and Cry1A toxins showed that major three 120, 110 and 100 kDa proteins bound with Cry1Aa and Ac, but not with Cry1Ab. Furthermore, the proteins were positive with anti aminopeptidase N antibody and the intensity and pattern of the binding were almost the same, respectively in the both straihs. Cry1Aa and Ac were shown to bind with the proteins at about 0.7 ng toxin/mm<SUP>2</SUP> membrane/10 min in both the strains using plasmon resonance. Their binding curves were almost the same each other. Cry1Ab, however, was not shown to bind with the BBMV proteins in both the strains.

    DOI： 10.11416/jsss.jsss72.0.66.0

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  • Polymorphism in rice amylases at an early stage of seed germination Reviewed

    Mitsunaga, S.-I., Kawakami, O., Numata, T., Yamaguchi, J., Fukui, K., Mitsui, T.

    Bioscience, Biotechnology and Biochemistry   65 ( 3 )   662 - 665   2001.12

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    DOI： 10.1271/bbb.65.662

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  • 新聞紙混合超高速食品汚泥発行法の研究―オガクズ混合法との比較研究―

    堀秀隆, 岩渕健一, Azwan Awang, 三ツ井敏明

    新潟大学農学部研究報告   53 ( 2 )   133 - 143   2001.11

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  • Development of monoclonal antiboby that recognizes N-linked complex glycan of plant glycoprotein.

    M. Watanabe, T. Kishimoto, H. Hori, T. Mitsui

    新潟大学農学部研究報告   53 ( 2 )   145 - 150   2001.3

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  • Enhancement of germination of sports from obligatory plant pathogen, Plasmodiophora brassicae causing clubroot disease

    S. Ogawa, H. Takahashi, T. Hayakawa, K. Ito, T. Mitsui, H. Hori, A. Kiso

    新潟大学農学部研究報告   54 ( 1 )   35 - 43   2001.3

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  • Rice α-mannosidase digesting the high mannose glycopeptide of glutelin Reviewed

    T. Kishimoto, H. Hori, D. Takano, Y. Nakano, M. Watanabe, T. Mitsui

    Physiologia Plantarum   112 ( 1 )   15 - 24   2001

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    DOI： 10.1034/j.1399-3054.2001.1120103.x

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  • Lipids of brush border membrane vesicles (BBMV) from Plutella xylostella resistant and susceptible to Cry1Ac δ-endotoxin of Bacillus thuringiensis Reviewed

    N. Selvamuthu Kumaraswami, Takeshi Maruyama, Sakiko Kurabe, Tadashi Kishimoto, Toshiaki Mitsui, Hidetaka Hori

    Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology   129 ( 1 )   173 - 183   2001

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    DOI： 10.1016/S1096-4959(01)00327-X

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  • Purification and Characterization of Golgi Membrane-Bound Nucleoside Diphosphatase from Suspension- Cultured Cells of Sycamore (Acer pseudoplatanus L.) Reviewed

    Satoshi Mikami, Ryo Suganuma, Hidetaka Hori, Toshiaki Mitsui

    Plant Biotechnology   18 ( 4 )   259 - 265   2001

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    DOI： 10.5511/plantbiotechnology.18.259

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  • Resting Spore of Plasmodiophora brassicae Proliferates only in the Callus of Clubroot Disease-Susceptible Turnip but Increases the PAL Activity in the Callus of Clubroot Disease-Resistant Turnip Reviewed

    Hideyuki Takahashi, Satoko Muraoka, I. T.O. Kimiko, Toshiaki Mitsui, Hidetaka Hori, Akira Kiso

    Plant Biotechnology   18 ( 4 )   267 - 274   2001

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    DOI： 10.5511/plantbiotechnology.18.267

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  • Separation of distinct compartments of rice Golgi complex by sucrose density gradient centrifugation Reviewed

    Satoshi Mikami, Hidetaka Hori, Toshiaki Mitsui

    Plant Science   161 ( 4 )   665 - 675   2001

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    DOI： 10.1016/S0168-9452(01)00455-1

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  • Alteration of starch-sucrose transition in germinating wheat seed under sodium chloride salinity. Reviewed

    M.A. Kashem, N. Sultana, T. Ikeda, T. Mitsui

    J. Plant Biol.   43 ( 3 )   121 - 127   2000.12

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  • Characterization of midgut fluids from Plutella xylostella resistant and susceptible to CrylAc toxin of Bacillus thuringiensis

    K. Ohtsu, T. Maruyama, H. Hama, T. Mitsui, H. Hori

    Bulletin of the Faculty of Agriculture, Niigata University   52 ( 2 )   115 - 127   2000.3

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  • Differential glycosylation of secretory and vacuolar glycoproteins in rice cell culture

    T. Mitsui, M. Watanabe, T. Kishimoto, S. Mikami, H. Hori, S. Kimura, K. Tomisawa, K. Takahashi, T. Hayakawa, I. Igaue

    新潟大学農学部研究報告   52 ( 2 )   101 - 147   2000

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  • Basic subunit of glutelin, rice major storage protein, has N-linked sugar side chain Reviewed

    Tadashi Kishimoto, Hidetaka Hori, Mayumi Watanabe, Toshiaki Mitsui

    Plant Biotechnology   17 ( 3 )   217 - 224   2000

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    DOI： 10.5511/plantbiotechnology.17.217

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  • GA3 and proline promote germination of wheat seeds by stimulating α-amylase at unfavorable temperatures Reviewed

    N. Sultana, T. Ikeda, T. Mitsui

    Plant Production Science   3 ( 3 )   232 - 237   2000

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    DOI： 10.1626/pps.3.232

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  • Possible involvement of phosphoinositide-Ca2+ signaling in the regulation of α-amylase expression and germination of rice seed (Oryza satira L.) Reviewed

    Mohammad A. Kashem, Kimiko Itoh, Satoru Iwabuchi, Hidetaka Hori, Toshiaki Mitsui

    Plant and Cell Physiology   41 ( 4 )   399 - 407   2000

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    DOI： 10.1093/pcp/41.4.399

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  • Role of the N-terminal region of the regulatory light chain in the dephosphorylation of myosin by myosin light chain phosphatase

    Ikebe, R., Reardon, S., Mitsui, T., Ikebe, M.

    Journal of Biological Chemistry   274 ( 42 )   30122 - 30126   1999.12

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    DOI： 10.1074/jbc.274.42.30122

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  • Differences in sugar regulation between two isoforms of alpha-amylase in suspension-cultured cells of rice. Reviewed

    N. Geshi, T. Mitsui, T. Akazawa, J. Yamaguchi

    J. Appl. Glycosci.   46 ( 2 )   111 - 119   1999.12

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  • Glutelin basic subunits have a mammalian mucin-type O-linked disaccharide side chain Reviewed

    T Kishimoto, M Watanabe, T Mitsui, H Hori

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   370 ( 2 )   271 - 277   1999.10

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  • Involvement of phosphoinositide-signaling on the regulation of α-Amylase secretion in suspension-cultured cells of rice

    T. Mitsui, M. A. Kashem, S. Iwabuchi, I kamimura, H. Hori, Y. Yoshida

    新潟大学農学部研究報告   52 ( 1 )   29 - 40   1999.5

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  • Sugar-controlled Ca2+ uptake and α-amylase secretion in cultured cells of rice (Oryza sativa L.) Reviewed

    Toshiaki Mitsui, Tadeusz Loboda, Akira Itoh, Taro Ikarashi

    Plant and Cell Physiology   40 ( 8 )   884 - 893   1999

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    DOI： 10.1093/oxfordjournals.pcp.a029618

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  • Identification and characterization of a novel α-Amylase isofome’N’ in germinating rice seed

    R. Datta, M. A. Kashem, T. Takamuki, T. Mitsui

    新潟大学農学部研究報告   51 ( 2 )   131 - 138   1999

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  • Sucrose-controlled transport and turnover of α-amylase in rice (Oryza sativa L.) cells Reviewed

    Toshiaki Mitsui, Tadeusz Loboda, Ikuko Kamimura, Hidetaka Hori, Kimiko Itoh, Shin-Ichiro Mitsunaga

    Plant and Cell Physiology   40 ( 8 )   773 - 783   1999

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    DOI： 10.1093/oxfordjournals.pcp.a029605

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  • Effects of (+)-8',8',8'-trifluoroabscisic acid on α-amylase expression and sugar accumulation in rice cells Reviewed

    Kashem, M.A., Hori, H., Itoh, K., Hayakawa, T., Todoroki, Y., Hirai, N., Ohigashi, H., Mitsui, T.

    Planta   205 ( 3 )   319 - 326   1998.12

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    DOI： 10.1007/s004250050326

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  • Two distinct types of silencing show different tissue specificity and stability in Wx gene silencing（proceedings) Reviewed

    K. Itoh, H. Ozaki, T. Mitsui, K. Shimamoto

    Agriculture Biotechnology: Laboratory, Field and Market   221 - 222   1998.12

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  • Effects of 8′,8′,8′-trifluoroabscisic acid on α-amylase secretion and sugar accumulation in rice cells Reviewed

    M. A. Kashem, K. Itoh, T. Hayakawa, T. Mitsui, Y. Todorold, N. Hirai, H. Ohigashi

    Nippon Nogeikagaku Kaishi   71   1 - 5   1997

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    DOI： 10.1271/nogeikagaku1924.71.sup_1

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  • The α-amylase multigene family

    Toshiaki Mitsui, Kimiko Itoh

    Trends in Plant Science   2 ( 7 )   255 - 261   1997

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    DOI： 10.1016/S1360-1385(97)86347-9

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  • Phosphate modulates sugar-controlled α-amylase secretion from rice suspension cell culture

    T. Loboda, T. Mitsui

    新潟大学農学部研究報告   49 ( 2 )   67 - 74   1997

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  • Synthesis, biological activity, and metabolism of 8′,8′,8′-trideuteroabscisic acid Reviewed

    Yasushi Todoroki, Sei-Ichi Nakano, Nobuhiro Hirai, Toshiaki Mitsui, Hajime Ohigashi

    Bioscience, Biotechnology and Biochemistry   61 ( 11 )   1872 - 1876   1997

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    DOI： 10.1271/bbb.61.1872

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  • Physicochemical and serological characterization of rice alpha-amylase isoforms and identification of their corresponding genes

    T Mitsui, J Yamaguchi, T Akazawa

    PLANT PHYSIOLOGY   110 ( 4 )   1395 - 1404   1996.4

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  • PURIFICATION OF PHOSPHOINOSITOL KINASE FROM SUSPENSION-CULTURED CELLS OF RICE (ORYZA-SATIVA L)

    K YOTSUSHIMA, T MITSUI, T HAYAKAWA

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   59 ( 10 )   1953 - 1955   1995.10

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  • A cell biochemical study on sugar-controlled alpha-amylase secretion in rice

    T Mitsui, K Yotsushima, Y Nabekura

    SUCROSE METABOLISM, BIOCHEMISTRY, PHYSIOLOGY AND MOLECULAR BIOLOGY   14   254 - 265   1995

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  • CORRELATION BETWEEN HIGH-TEMPERATURE DEPENDENCE OF SMOOTH-MUSCLE MYOSIN LIGHT-CHAIN PHOSPHATASE-ACTIVITY AND MUSCLE-RELAXATION RATE

    T MITSUI, T KITAZAWA, M IKEBE

    JOURNAL OF BIOLOGICAL CHEMISTRY   269 ( 8 )   5842 - 5848   1994.2

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  • Structure and function of the Golgi complex in rice cells. II.Purification and characterization of Golgi membrane-bound nucleoside diphosphatase

    T. Mitsui, M. Honma, T. Kondo, N. Hashimoto, S. Kimura, I. Igaue

    Plant Physiology   106 ( 1 )   119 - 125   1994

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    DOI： 10.1104/pp.106.1.119

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  • BIOSYNTHESIS AND SECRETION OF ALPHA-AMYLASE BY RICE SUSPENSION-CULTURED CELLS - PURIFICATION AND CHARACTERIZATION OF ALPHA-AMYLASE ISOZYME-H

    T MITSUI, Y UEKI, IGAUE, I

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   31 ( 6 )   863 - 874   1993.11

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  • STRUCTURE AND FUNCTION OF THE GOLGI-COMPLEX IN RICE CELLS - CHARACTERIZATION OF GOLGI MEMBRANE-GLYCOPROTEINS

    S KIMURA, M YAMADA, IGAUE, I, T MITSUI

    PLANT AND CELL PHYSIOLOGY   34 ( 6 )   855 - 863   1993.9

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  • PURIFICATION AND CHARACTERIZATION OF MEMBRANE-BOUND INOSITOL PHOSPHOLIPID-SPECIFIC PHOSPHOLIPASE-C FROM SUSPENSION-CULTURED RICE (ORYZA-SATIVA L) CELLS - IDENTIFICATION OF A REGULATORY FACTOR

    K YOTSUSHIMA, T MITSUI, T TAKAOKA, T HAYAKAWA, GAUE, I

    PLANT PHYSIOLOGY   102 ( 1 )   165 - 172   1993.5

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  • PURIFICATION OF BETA-GLUCAN SYNTHASE-II FROM SUSPENSION-CULTURED RICE CELLS

    KURIBAYASHI, I, T MORITA, T MITSUI, IGAUE, I

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   57 ( 4 )   682 - 684   1993.4

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  • PURIFICATION, CHARACTERIZATION AND LOCALIZATION OF RICE UDP-GLUCOSE PYROPHOSPHORYLASE

    S KIMURA, T MITSUI, T MATSUOKA, IGAUE, I

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   30 ( 6 )   683 - 693   1992.11

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  • LOCALIZATION OF BINDING-SITES FOR PEANUT, ULEX-EUROPAEUS, AND CASTOR BEAN LECTIN IN THE RICE GOLGI-COMPLEX

    S KIMURA, T MITSUI, IGAUE, I

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   56 ( 10 )   1656 - 1657   1992.10

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  • PURIFICATION AND CHARACTERIZATION OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C IN SUSPENSION-CULTURED CELLS OF RICE (ORYZA-SATIVA L)

    K YOTSUSHIMA, K NAKAMURA, T MITSUI, IGAUE, I

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   56 ( 8 )   1247 - 1251   1992.8

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  • PURIFICATION AND CHARACTERIZATION OF SMOOTH-MUSCLE MYOSIN-ASSOCIATED PHOSPHATASE FROM CHICKEN GIZZARDS

    T MITSUI, M INAGAKI, M IKEBE

    JOURNAL OF BIOLOGICAL CHEMISTRY   267 ( 23 )   16727 - 16735   1992.8

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  • PREPARATION OF GOLGI MEMBRANE-FRACTIONS CONTAINING LECTIN-BINDING SITES FROM SUSPENSION-CULTURED RICE CELLS

    S KIMURA, K MATSUDA, T MITSUI, IGAUE, I

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   56 ( 2 )   340 - 341   1992.2

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  • Ultrastructure of Golgi complex and partially coated reticulum in suspension-cultured cell of rice

    S. Kimura, T. Mitsui, I, Igaue

    新潟大学農学部研究報告   43 ( 43 )   83 - 91   1991

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  • STRUCTURE AND BIOSYNTHESIS OF THE XYLOSE-CONTAINING CARBOHYDRATE MOIETY OF RICE ALPHA-AMYLASE

    M HAYASHI, A TSURU, T MITSUI, N TAKAHASHI, H HANZAWA, Y ARATA, T AKAZAWA

    EUROPEAN JOURNAL OF BIOCHEMISTRY   191 ( 2 )   287 - 295   1990.7

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  • ISOLATION AND CHARACTERIZATION OF GOLGI MEMBRANES FROM SUSPENSION-CULTURED CELLS OF RICE (ORYZA-SATIVA-L)

    T MITSUI, S KIMURA, IGAUE, I

    PLANT AND CELL PHYSIOLOGY   31 ( 1 )   15 - 25   1990.1

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  • Trichosporon penicillatum のペクチナーゼによる植物葉肉プロトプラストの単離.

    三ツ井敏明, 橋本憲明, 出口恭平, 平野真弓, 伊賀上郁夫

    植物培養細胞   7 ( 1 )   14 - 18   1990

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Plant Cell and Molecular Biology  

    Plant mesophyll protoplasts with a endo-polygalacturonase produced by <i>Trichosporon penicillatum</i> SNO-3 was isolated. The following mesophyll tissues of shoot were used for the isolation: <i>Tagetes minuta</i>, <i>Brassica rapa</i>, <i>Raphanus sativus</i>, <i>Lactuca sativa</i>, <i>Triticum aestivum</i>, <i>Secale cereale</i>, <i>Hordeum vulgare</i>, <i>Panicum crusgalli</i>, <i>Avena sativa</i>, <i>Zeamays</i> and <i>Oryza sativa</i>. Pectinase SE-150, a partially purified endo-polygalacturonase, combined with cellulase ONOZUKA RS liberated protoplasts from all mesophyll tissues tested.<br>The protoplast yields were 1-4×10<sup>6</sup> per gram fresh weight. Cell division and colony formation from lettuce protoplasts obtained with a pectinase SE-150 enzyme system were also observed. The percentage of colony formation on the 14th day after incubation in a culturing medium was estimated to be approximately 18%.

    DOI： 10.5511/plantbiotechnology1984.7.14

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  • SUGAR NUCLEOTIDE CONTENTS IN SUSPENSION-CULTURED RICE CELLS

    T MITSUI, T SADO, A TERADA, IGAUE, I

    AGRICULTURAL AND BIOLOGICAL CHEMISTRY   53 ( 7 )   1991 - 1993   1989.7

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  • REGULATORY ROLE OF CA-2+ ON THE LEVEL OF ALPHA-AMYLASE MESSENGER-RNA IN RICE SEED

    M HAYASHI, T MITSUI, T AKAZAWA

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   27 ( 3 )   349 - 354   1989.5

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  • ENZYMATIC MECHANISM OF STARCH BREAKDOWN IN GERMINATING RICE SEEDS .21. PREFERENTIAL SECRETION OF R-TYPE ALPHA-AMYLASE MOLECULES IN RICE SEED SCUTELLUM AT HIGH-TEMPERATURES

    T MITSUI, T AKAZAWA

    PLANT PHYSIOLOGY   82 ( 4 )   880 - 884   1986.12

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  • GOLGI-SPECIFIC LOCALIZATION OF TRANSGLYCOSYLASES ENGAGED IN GLYCOPROTEIN-BIOSYNTHESIS IN SUSPENSION-CULTURED CELLS OF SYCAMORE (ACER-PSEUDOPLATANUS L)

    MS ALI, T MITSUI, T AKAZAWA

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   251 ( 2 )   421 - 431   1986.12

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  • SECONDARY MODIFICATION OF CARBOHYDRATE CHAINS IN ALPHA-AMYLASE MOLECULES SYNTHESIZED IN RICE SCUTELLUM

    T MITSUI, T AKAZAWA

    PHYSIOLOGIE VEGETALE   24 ( 6 )   629 - 638   1986.11

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  • Possible roles of calcium and calmodulin in the biosynthe sis and secretion of α-amylase in rice scutellar epithelium

    T. Mitsui, J.T. Christeller, I. Hara-Nishimura, T. Akazawa

    Plant Physiol   75 ( 1 )   880 - 884   1985

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  • ISOLATION AND CHARACTERIZATION OF GOLGI MEMBRANES FROM SUSPENSION-CULTURED CELLS OF SYCAMORE (ACER-PSEUDOPLATANUS L)

    MS ALI, M NISHIMURA, T MITSUI, T AKAZAWA, K KOJIMA

    PLANT AND CELL PHYSIOLOGY   26 ( 6 )   1119 - 1133   1985

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  • BIOSYNTHESIS OF RICE SEED ALPHA-AMYLASE - 2 PATHWAYS OF AMYLASE SECRETION BY THE SCUTELLUM

    T MITSUI, T AKAZAWA, JT CHRISTELLER, AM TARTAKOFF

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   241 ( 1 )   315 - 328   1985

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  • ENZYMIC MECHANISM OF STARCH BREAKDOWN IN GERMINATING RICE SEEDS .13. POSSIBLE ROLES OF CALCIUM AND CALMODULIN IN THE BIOSYNTHESIS AND SECRETION OF ALPHA-AMYLASE IN RICE SEED SCUTELLAR EPITHELIUM Reviewed

    T MITSUI, JT CHRISTELLER, HARANISHIMURA, I, T AKAZAWA

    PLANT PHYSIOLOGY   75 ( 1 )   21 - 25   1984

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  • ENZYMIC MECHANISM OF STARCH BREAKDOWN IN GERMINATING RICE SEEDS .15. IMMUNOCHEMICAL STUDY ON MULTIPLE FORMS OF AMYLASE

    J DAUSSANT, S MIYATA, T MITSUI, T AKAZAWA

    PLANT PHYSIOLOGY   71 ( 1 )   88 - 95   1983

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  • バイオスティミュラントハンドブック : 植物の生理活性プロセスから資材開発、適用事例まで

    Marouane BASLAM, Murat AYCAN, 三ツ井敏明（ Role： Joint author ,  第1編 第5章 第3節(pp.165-172) 三ツ井敏明 第2編 第5章 第5節(pp.381-390)Marouane BASLAM, Murat AYCAN, 三ツ井敏明）

    エヌ・ティー・エス  2022.4  （ ISBN:9784860437695 ）

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    Total pages：iii, xiii, 460, xiip, 図版vip   Language：Japanese

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  • The Future of Rice Demand: Quality Beyond Productivity: Chapter20 Proteomic for Quality: Mining the Proteome as a Strategy to Elucidate the Protein Complex Applied for Quality Improvement

    Marouane BASLAM, Toshiaki MITSUI（ Role： Joint author ,  Chapter20 Proteomic for Quality: Mining the Proteome as a Strategy to Elucidate the Protein Complex Applied for Quality Improvement）

    Springer  2020.3 

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  • Nutrient Dynamics for Sustainable Crop: Production :Use of Organic and Biological Fertilizers as Strategies to Improve Crop Biomass, Yields and Physicochemical Parameters of Soil

    Abdelilah MEDDICH, Khalid OUFDOU, Abderrahim BOUTASKNIT, Anas RAKLAMI, Abdelilah TAHIRI, Raja BEN-LAOUANE, Mohamed AIT-EL-MOKHTAR, Mohamed ANLI, Toshiaki MITSUI, Said WAHBI, Marouane BASLAM（ Role： Joint author）

    Springer  2019.9  （ ISBN:9789811386596 ）

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    Total pages：18   Responsible for pages：247-288   Language：English Book type：Scholarly book

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  • Soil Biology 52, Root Biology Chapter9: Optimizing growth and tolerance of date palm (Phoenix dactylifera L.) to drought, salinity and vascular fusarium-induced wilt (Fusarium oxysporum) by application of Arbuscular Mycorrhizal Fungi (AMF)

    Abdelilaf MEDDICH, Mohamed Ait EL-MOKHTAR, Widad BOURZIK, Toshiaki MITSUI, Marouane BASLAM, Mohamed HAFIDI（ Role： Joint author）

    Springer-Verlag GmbH  2018.5 

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  • 米の外観品質・食味－最新研究と改善技術－

    三ツ井敏明, 金古堅太郎, 白矢武士（ Role： Sole author ,  第15章 高温耐性イネの開発戦略－澱粉代謝関連酵素の細胞分子生物学の視点から－）

    養賢堂  2018.2 

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    Total pages：478   Responsible for pages：223-236   Language：Japanese Book type：Scholarly book

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  • Plant Membrane Proteomics: Metods and protocols Chapter6:Proteomic Analysis of Rice Golgi Membranes Isolated by Floating Through Discontinuous Sucrose Density Gradient

    Kazusato OIKAWA, Takuya INOMATA, Yoshitoshi HIRAO, Tadashi YAMAMOTO, Marouane BASLAM, Kentaro KANEKO, Toshiaki MITSUI（ Role： Joint author）

    Humana Press  2017.11 

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    Total pages：280   Responsible for pages：91-105   Language：English Book type：Scholarly book

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  • Plant Proteomics Methods and Protocols 2nd edition Chapter44:Rapid and high-throughput N-glycomic analysis of plant glycoproteins.

    Kentaro KANEKO, Takeshi SHIRAYA, Toshiaki MITSUI, Shin-ichiro NISHIMURA（ Role： Joint author）

    Humana press, New Jersey  2013.10  （ ISBN:9781627036306 ）

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    Responsible for pages：645-653   Language：English Book type：Scholarly book

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  • Plant Proteomics Methods and Protocols 2nd edition Chapter32:Quantitative proteomic analysis of intact plastids.

    Takeshi SHIRAYA, Kentaro KANEKO, Toshiaki MITSUI（ Role： Joint author）

    Humana press, new Jersey  2013.10  （ ISBN:9781627036306 ）

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    Responsible for pages：469-480   Language：English Book type：Scholarly book

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  • プロテオミクス辞典 日本プロテオーム学会編

    三ツ井敏明, 金古堅太郎ほ（ Role： Joint author）

    講談社  2013.9  （ ISBN:9784061538931 ）

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  • 発芽生物学

    種生物学会（ Role： Joint author ,  貯蔵デンプン分解の分子機構）

    文一総合出版  2009.4 

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  • Research Communications in Biochemistry, Cell and Molecular Biology

    Komatsu, S, Ahsan, N, Mitsui, T, Nozu, Y（ Role： Joint author）

    PJD publications limited  2009.3 

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  • 朝倉植物生理学講座1．植物細胞

    三ツ井敏明（ Role： Joint author ,  ゴルジ体）

    朝倉書店  2002.9  （ ISBN:4254176554 ）

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  • 最新運動生理科学実験法-分子・細胞・組織レベルからのアプローチ-

    三ツ井敏明, 光永伸一郎（ Role： Joint author ,  モノクローナル抗体の作成法とその応用）

    大修館書店  1998.7  （ ISBN:446926394X ）

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  • 植物細胞工学シリーズ９. 植物のタンパク質実験プロトコール−遺伝子と組織から迫るタンパク質の機能と構造

    三ツ井敏明, 岸本正（ Role： Joint author ,  173-178 糖タンパク質の分析）

    秀潤社  1998.3 

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  • 種子のバイオサイエンス

    種子生理生化学研究会編（ Role： Joint author ,  糖鎖分解酵素遺伝子の発現と分泌）

    学会出版センター  1995.3  （ ISBN:476228789X ）

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  • The Biochemistry of Plants. A Comprehensive Treatise. Vol.14 ,Carbohydrates.

    Takashi Akazawa, Toshiaki Mitsui, Makoto Hayashi（ Role： Joint author ,  Recent Progress in alpha-amylase Biosynthesis）

    Academic Press,Inc  1988.3  （ ISBN:0126754144 ）

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  • New Approaches to Research on Cereal Carbohydrate

    Takashi Akazawa, Toshiaki Mitsui（ Role： Joint author）

    Elsevier Science Publishers  1985 

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  • グリコーゲン代謝酵素の欠損及びイネデンプン合成酵素の発現が大腸菌のグリコーゲン構造と代謝に及ぼす影響の研究

    伊藤可那, 伊藤可那, 福島真美, 松木順子, 花城勲, ZABALZA Goizeder Almagro, 高橋秀行, 金古堅太郎, 三ツ井敏明, POZUETA=ROMERO Javier, 伊藤紀美子

    応用糖質科学   10 ( 4 )   2020

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  • Applied Molecular and Cell Biological Studies of Starch-degrading Enzymes in Rice Invited

    Toshiaki MITSUI

    Bulletin of Appllied Glycoscience   9 ( 1 )   3 - 10   2019.2

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  • イネの種子登熟におけるオートファジーの役割

    瀬良ゆり, 花俣繁, 坂本真吾, 小野聖二郎, 三井悠大, 金古堅太郎, 佐生愛, 北畑信隆, 佐治光, 藤田直子, 増村威宏, 三ツ井敏明, 野々村賢一, 光田展隆, 来須孝光, 朽津和幸

    超分散知能システム研究部門平成29年度研究成果概要集   33 - 36   2018.3

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  • イネ由来α-アミラーゼの触媒反応機構に対する構造的洞察

    落合秋人, 菅井寛, 田中孝明, 谷口正之, 三ツ井敏明

    日本農芸化学会大会講演要旨集(Web)   2018   2018

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  • Chalking Mechanism of Rice Grain under High Temperature Stress

    112 ( 5 )   323 - 329   2017.5

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  • イネ由来α-アミラーゼの糖結合部位に対する機能解析

    山田大貴, 落合秋人, 荻原寛和, 田中孝明, 三ツ井敏明, 谷口正之

    日本生物工学会大会講演要旨集   69th   2017

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  • A Molecular Physiological Mechanism of Rice Grain Chalking by High Temperature Stress Invited Reviewed

    Toshiaki MITSUI

    KAGAKU TO SEIBUTSU   54 ( 4 )   254 - 259   2016.3

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  • 植物細胞内の代謝を効率化させるオルガネラ相互作用

    及川和聡, 細川陽一郎, 三ツ井敏明, 西村幹夫

    応用糖質科学   6 ( 3 )   2016

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  • 次世代シーケンス解析に向けた高純度のイネ葉緑体DNA精製法

    高松壮, 大西孝幸, 猪俣拓也, 及川和聡, 木下哲, 三ツ井敏明

    日本農芸化学会大会講演要旨集(Web)   2016   2016

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  • イネ由来α-アミラーゼの立体構造とその多機能性の解析（ミニレビュー）

    落合秋人, 谷口正之, 三ツ井敏明

    応用糖質科学   5 ( 3 )   162 - 164   2015.8

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  • Ca-5 質量分析器を用いた澱粉物性の異なるイネ品種間のプロテオーム解析(澱粉の生合成,一般講演,一般社団法人日本応用糖質科学会平成27年度大会(第64回))

    伊藤 紀美子, 渡邊 茉莉, 金古 堅太郎, 二瓶 正崇, 三ツ井 敏明

    応用糖質科学 : 日本応用糖質科学会誌   5 ( 3 )   B47   2015.8

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  • Aa-6 登熟期間のCO_2濃度がコメ外観品質に与える影響(澱粉の性質,一般講演,一般社団法人日本応用糖質科学会平成27年度大会(第64回))

    猪俣 拓也, 金古 堅太郎, 涌井 翔太郎, 若松 和, 三ツ井 敏明

    応用糖質科学 : 日本応用糖質科学会誌   5 ( 3 )   B36   2015.8

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  • Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis (vol 1, 15035, 2015)

    Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa, Mikio Nishimura

    NATURE PLANTS   1 ( 4 )   2015.3

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  • プラスチド局在スーパーオキシドジスムターゼはイネ登熟期における高温被害米の発生を低減する

    金古堅太郎, 佐々木麻衣子, 白矢武士, 高松壮, 及川和聡, 古川豊和, 三ツ井敏明

    日本農芸化学会大会講演要旨集(Web)   2015   2015

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  • 光合成を効率化するオルガネラ相互作用

    及川和聡, 真野昌二, 三ツ井敏明, 細川陽一郎, 西村幹夫

    応用糖質科学   5 ( 3 )   2015

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  • ゴルジ体-葉緑体間タンパク質輸送に関与するイネ膜タンパク質TMP1の解析

    及川和聡, 伊東七実子, 中山勇希, 石山隆一, 金古堅太郎, 古賀彩, 谷内智子, 高松壮, 三ツ井敏明, 三ツ井敏明

    日本植物生理学会年会要旨集   56th   2015

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  • 形態変化を伴ったオルガネラ間相互作用の解析~オルガネラ間接着力測定の試み~

    及川和聡, 真野昌二, 近藤真紀, 坂本亘, 三ツ井敏明, 飯野敬矩, 細川陽一郎, 西村幹夫

    日本植物学会大会研究発表記録   79th   2015

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  • Aa-1 イネのヌクレオチドピロホスファターゼ/ホスホジエステラーゼ1(NPP1)の欠損が糖代謝に及ぼす影響(澱粉の生合成,一般講演,日本応用糖質科学会平成26年度大会(第63回))

    増井 貴広, 猪俣 拓也, 涌井 翔太郎, 金古 堅太郎, 三ツ井 敏明

    応用糖質科学 : 日本応用糖質科学会誌   4 ( 3 )   B32   2014.8

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  • FS-2 イネ由来α-アミラーゼの立体構造とその多機能性の解析(第3回応用糖質フレッシュシンポジウム,日本応用糖質科学会平成26年度大会(第63回))

    落合 秋人, 谷口 正之, 三ツ井 敏明

    応用糖質科学 : 日本応用糖質科学会誌   4 ( 3 )   B64   2014.8

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  • Involvement of Golgi/Plastid-type Superoxide Dismutase in High Temperature Stress Tolerance during Grain Filling of Rice

    三ツ井敏明, 三ツ井敏明, 白矢武士, 佐々木麻衣子, 丸山達也, 高松壮, 及川和聡, 金古堅太郎

    日本プロテオーム学会大会プログラム・抄録集   2014 (Web)   2014

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  • イネ由来α-アミラーゼの立体構造とその熱安定性に関与する構造要因の解析

    落合秋人, 菅井寛, 伊東孝祐, 内海利男, 田中孝明, 谷口正之, 三ツ井敏明

    日本生化学会大会(Web)   87th   2014

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  • 2P-042 Crystal Structure of α-Amylase AmyI-1 from Oryza sativa

    Ochiai Akihito, Sugai Hiroshi, Harada Kazuki, Itoh Kousuke, Uchiumi Toshio, Tanaka Takaaki, Taniguchi Masayuki, Mitsui Toshiaki

    66   117 - 117   2014

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  • Regulation of α-amylase expression in rice and grain quality of rice

    Mitsui Toshiaki

    48   12 - 12   2013.10

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  • Bp-3 乳白米粒の澱粉・プロテオーム解析(澱粉の生合成,一般講演,日本応用糖質科学会平成25年度大会(第62回))

    佐々木 麻衣子, 金古 堅太郎, 丸山 達也, 白矢 武士, 澤田 隆行, 三ツ井 敏明

    応用糖質科学 : 日本応用糖質科学会誌   3 ( 3 )   B35   2013.8

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  • Dissection of the mechanism underlying production of chalky rice grains by ripening under high temperature

    YAMAKAWA Hiromoto, HAKATA Makoto, KURODA Masaharu, MIYASHITA Tomomi, YAMAGUCHI Takeshi, KOJIMA Mikiko, SAKAKIBARA Hitoshi, MITSUI Toshiaki

    1 ( 4 )   24 - 27   2013.4

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  • Development of Rice Plants with Reduced Chalky Grains by Manipulation of Starch Metabolism Synthesis or Degradation, That Is the Question

    YAMAKAWA Hiromoto, HAKATA Makoto, KURODA Masaharu, MIYASHITA Tomomi, YAMAGUCHI Takeshi, KOJIMA Mikiko, SAKAKIBARA Hitoshi, MITSUI Toshiaki

    82   460 - 461   2013.3

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  • Proteomic Analysis of Rice Seeds Grown Under High Temperature Stress during Grain Filling

    MITSUI Toshiaki, KANEKO Kentaro, MARUYAMA Tatsuya, SASAKI Maiko, SHIRAYA Takeshi, YAMAKAWA Hiromoto

    82   456 - 457   2013.3

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  • 朱鷺のプロテオーム解析

    小柳充, 小柳充, 小柳充, 金古堅太郎, 金子良則, 山城秀昭, 山城秀昭, 山田宜永, 山田宜永, 杉山稔恵, 杉山稔恵, 佐々木麻衣子, 松田洋一, 三ツ井敏明

    日本生化学会大会(Web)   86th   2013

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  • 83. Regulation of poor-nutrition stress-induced fl owering by salicylic acid and PnFT2 in Pharbitis nil

    Wada Kaede, Yamada Mizuki, Koshio Aya, Kaneko Kentaro, Shiraya Takeshi, Hoshino Atsushi, Sakai Tatsuya, Mitsui Toshiaki, Kato Akira, Takeno Kiyotosi

    47   100 - 100   2012.10

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  • Cloning, expression, and intracellular localization of rice SUMO genes

    Ikarashi Yuta, Noguchi Natsuki, Attia Kotb, Kitajima-Koga Aya, Mitsui Toshiaki, Itoh Kimiko

    新潟大学農学部研究報告   65 ( 1 )   77 - 83   2012.9

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    Small ubiquitin-related modifier (SUMO) is a type of ubiquitin-like proteins and regulates various protein functions through post-translational modification. The SUMO precursor proteins are processed by C-terminal cleavage reaction at the end of the di-glycine(GG) motif, and then activated and bind to substrate proteins by a series of enzymatic reaction. We cloned SUMO1-3 genes in rice, and analyzed the transient expression and intracellular localization of the DsRed fusion proteins, DsRed:SUMO1, 2, and 3 in onion epidermal cells by using confocal laser scanning microscopy. The DsRed signals from DsRed:SUMO1 and DsRed:SUMO2 were detected both in nuclei and cytoplasmic location, but not in nucleoli. In the case of DsRed:SUMO3, the DsRed signal was detected mainly in nucleus, and formed sub-nuclear domain like structure. We also tested effect of the GG motif on intracellular localization of SUMO proteins. The GG deletion mutant vectors, pDsRed:SUMO1ΔGG, pDsRed:SUMO2ΔGG, and pDsRed:SUMO3ΔGG were constructed and transiently expressed in onion epidermal cells. Result showed that the deletion mutation of GG motif suppressed the accumulation of the DsRed:SUMOΔGG proteins in the nucleus. These results indicated that the C-terminal processing of OsSUMO precursor proteins are necessary for OsSUMO localization to nucleus in onion epidermal cells.Small-ubiquitin related modifier (SUMO)はユビキチン様タンパク質の一種であり、翻訳後修飾により多様なタンパク質の機能を調節する。SUMO 前駆体タンパク質はＣ末側に存在するジグリシン（GG）モチーフの末端でプロセシングされ、一連の酵素反応により活性化され基質タンパク質に結合する。私たちはイネSUMO1-3遺伝子を単離し、DsRed 融合タンパク質であるDsRed:SUMO1, ２、および３をタマネギ表皮細胞において一過的に発現させ、その細胞局在性を共焦点レーザー顕微鏡により解析した。DsRed:SUMO1およびDsRed:SUMO2のシグナルは核と細胞質にも分布したが、核小体には観察されなかった。DsRed:SUMO3の場合は、主に核に局在し、そのシグナルは細胞核ドメイン様構造を形成した。私たちはまた、GGモチーフ欠失変異細胞内局在性に関わる影響をテストした。GG モチーフ欠失変異を持つベクター、pDsRed:SUMO1ΔGG、pDsRed:SUMO2Δ GG、およびpDsRed:SUMO3ΔGG を構築し、タマネギ表皮細胞において発現させた。GG モチーフの欠失変異はDsRed:SUMO Δ GG タンパク質の核への集積を抑制した。これらの結果から、OsSUMO 前駆体タンパク質のＣ末端のプロセシングがタマネギ表皮細胞におけるOsSUMO タンパク質の核への局在性に必要であることが示唆された。

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  • Bp1-6 高温ストレスにより発生した白未熟米粒の定量的プロテオーム解析(澱粉の生合成と代謝,一般講演,日本応用糖質科学会平成24年度大会(第61回))

    丸山 達也, 佐々木 麻衣子, 白矢 武士, 金古 堅太郎, 三ツ井 敏明

    応用糖質科学 : 日本応用糖質科学会誌   2 ( 3 )   B36   2012.8

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  • FS-3 α-アミラーゼの抑制はイネの高温登熟障害を軽減する(第1回応用糖質フレッシュシンポジウム〜若手研究者ネットワークの構築に向けて〜,日本応用糖質科学会平成24年度大会(第61回))

    羽方 誠, 黒田 昌治, 三ツ井 敏明, 山川 博幹

    応用糖質科学 : 日本応用糖質科学会誌   2 ( 3 )   B62   2012.8

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  • 米の外観品質・食味研究の最前線―高温耐性イネの開発戦略（澱粉代謝関連酵素の細胞分子生物学の視点から）

    三ツ井敏明

    農業と園芸   8 ( 6 )   627 - 634   2012.6

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  • Biochemical Characterization of the Rice Kinesin O12 and N14 Belonging to Kinesin-14 Family

    Nozomi Umezu, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta

    BIOPHYSICAL JOURNAL   102 ( 3 )   369A - 369A   2012.1

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  • イネヌクレオチドピロホスファターゼ/ホスホジエステラーゼのプラスチド局在に関する研究

    梅澤幸歩, 金古堅太郎, 甲州努, 石本卓也, 伊藤紀美子, 林八寿子, 豊岡公徳, 三ツ井敏明

    日本農芸化学会大会講演要旨集(Web)   2012   2012

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  • Characterization of the Plant-Specific Rice Kinesins

    Nozomi Umezu, Nobuhisa Umeki, Nobue Hanzawa, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta

    BIOPHYSICAL JOURNAL   100 ( 3 )   121 - 121   2011.2

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  • Plastid targeting of NPP glycoprotein from the golgi apparatus through the secretory pathway in rice cell

    Kaneko Kentaro, Kosyu Tsutomu, Umezawa Yukiho, Ishimoto Takuya, Amano Maho, Nishimura Shin-Ichro, Mitsui Toshiaki

    Abstracts for Annual Meeting of Japanese Proteomics Society   2011 ( 0 )   139 - 139   2011

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    DOI： 10.14889/jhupo.2011.0.139.0

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  • <i>N</i>-glycome of rice plastidial nucleotide pyrophosphatase/phosphodiesterases: Functional glycoproteins are transported from the late compartments of the Golgi apparatus to the plastid

    Mitsui Toshiaki, Pozueta-Romero Javier, Kaneko Kentaro, Koushu Tsutomu, Umezawa Sachiho, Koga-Kitajima Aya, Shiraya Takeshi, Hirose Kazuko, Amano Maho, Nishimura Shin-ichiro

    Abstracts for Annual Meeting of Japanese Proteomics Society   2011 ( 0 )   65 - 65   2011

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    DOI： 10.14889/jhupo.2011.0.65.0

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  • イネヌクレオチドピロホスファターゼ/ホスホジエステラーゼのプラスチド局在化機構に関する研究

    梅澤幸歩, 金古堅太郎, 甲州努, 石本卓也, 伊藤紀美子, 林八寿子, 豊岡公徳, 三ツ井敏明

    生化学   2011

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  • Biosynthesis and degradation of starch

    「Toshiaki Mitsui」「Kimiko Itoh」「Hidetaka Hori」「Hiroyuki Ito」

    新潟大学農学部研究報告   62 ( 2 )   49 - 73   2010.5

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  • イネにおけるプラスチド局在糖タンパク質ヌクレオチドピロホスファターゼの糖鎖構造解析

    金古堅太郎, 甲州努, 梅澤幸歩, 北嶋(古賀)彩, 天野麻穂, 西村紳一郎, 豊岡公徳, 伊藤紀美子, 三ツ井敏明

    生化学   82 ( 9 )   2010

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  • Heat shock and grain damage under high temperature during ripening of rice

    MITSUI Toshiaki, YAMAKAWA Hiromoto

    32 ( 7 )   20 - 25   2009.7

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  • GAMYB遺伝子の転写後・翻訳後調節の解析

    五十嵐雄太, 宮嵜敬弘, 近藤尚, 永井理子, 南條洋平, 三ツ井敏明, 伊藤紀美子

    生化学   4P-0786   2008

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  • イネ糖タンパク質α-アミラーゼI-1のゴルジ体-プラスチド間輸送に関する研究

    北嶋彩, 岡田久夫, 浜田侑紀, 豊岡公徳, 浅妻悟, 浅妻悟, 松岡健, 松岡健, 中野明彦, 中野明彦, 三ツ井敏明

    生化学   2008

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  • イネ糖タンパク質α-アミラーゼI-1のプラスチドターゲティング:ゴルジ-プラスチド間輸送の解析

    北嶋彩, 唐橋あゆみ, 高田頌, 豊岡公徳, 浅妻悟, 松岡健, 松岡健, 中野明彦, 中野明彦, 三ツ井敏明

    日本植物生理学会年会要旨集   49th   2008

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  • Secretory Vesicle Cluster:トランスゴルジネットワーク由来の分泌系後期で働く新奇構造体

    松岡健, 松岡健, 豊岡公徳, 浅妻悟, 浅妻悟, 後藤友美, 三ツ井敏明

    日本植物生理学会年会要旨集   49th   2008

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  • Light-responsive changes in intracellular localization of rice homolog of CTR9, a component of yeast PAF1 complex

    Takeshi Shiraya, Aya Kitajima, Maho Tozawa, Changhong Guo, Hiroshi Ban, Naoki Yamamoto, Toshiaki Mitsui, Toshisuke Iwasaki

    2007.12

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  • タンパク質のプラスチドターゲティング

    三ツ井敏明

    化学と生物   45   461 - 467   2007.7

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  • Novel Chloroplast Protein Targeting System

    MITSUI Toshiaki

    Bioscience & industry   65 ( 6 )   296 - 298   2007.6

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  • 新しい葉緑体タンパク質輸送システム

    三ツ井敏明

    バイオサイエンスとインダストリー   65   30 - 32   2007.6

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  • Crystal structure of ADP bound motor domain of the rice kinesin K16 reveals a novel conformation

    Shinsaku Maruta, Nobuhisa Umeki, Nozomi Umezu, Yuko Kubo, Zui Fujimoto, Toshiaki Mitsui

    BIOPHYSICAL JOURNAL   497A - 497A   2007.1

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  • Characterization of the rice specific kinesins and interaction with fluorescent ATP analogue

    Nozomi Umezu, Yoko Kubo, Nobuhisa Umeki, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta

    BIOPHYSICAL JOURNAL   499A - 499A   2007.1

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  • Rice Golgi proteome: Characteristics of N-acetylglucosaminyltransferase I associated Golgi membranes in cells overexpressing glycoproteins

    Tsuyoshi Asakura, Hiroki Katamine, Makoto Aoyama, Shota Hirose, Yumi Namekata, Toshiaki Mitsui

    PLANT AND CELL PHYSIOLOGY   48   S23 - S23   2007

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  • N‐結合型糖鎖を有するNucleotide Pyrophosphatase/Phosphodiesterase(OsNNP1)のプラスチド局在について

    金古堅太郎, 岡宏匡, 五十嵐憲子, 南條洋平, JAVIER Pozueta‐Romero, 三ツ井敏明

    日本植物生理学会年会要旨集   47th   168   2006.3

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  • Investigation of transport pathway of rice alpha-amylase to plastid

    S Asatsuma, A Kitajima, C Sawada, M Takeuchi, A Nakano, T Mitsui

    PLANT AND CELL PHYSIOLOGY   47   S89 - S89   2006

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  • Rice Golgi proteome: Analysis of GFP-syp31 labeled cis Golgi membrane

    T Asakura, S Hirose, H Katamine, M Sato, M Fujiwara, K Shimamoto, F Hori, T Mitsui

    PLANT AND CELL PHYSIOLOGY   47   S26 - S26   2006

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  • Characterization of a plastidial N-glycosylated nucleotide pyrophosphatase/phospliodiesterase in rice

    K Kaneko, H Oka, N Ikarashi, Y Nanjo, J Pozueta-Romero, T Mitsui

    PLANT AND CELL PHYSIOLOGY   47   S89 - S89   2006

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  • デンプン代謝からみた白未熟粒発生メカニズム(研究の現状)．

    三ツ井敏明, 福山利範

    農業技術   60 ( 10 )   447 - 452   2005.12

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  • Purification and characterization of the plant specific kinesin

    N Umeki, Y Nakayama, K Kondo, T Mitsui, S Maruta

    BIOPHYSICAL JOURNAL   88 ( 1 )   651A - 651A   2005.1

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  • Conformational change of the unique loop L5 of rice kinesin

    N Umeki, Y Nakayama, K Kondo, T Mitsui, S Maruta

    BIOPHYSICAL JOURNAL   88 ( 1 )   648A - 648A   2005.1

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  • Functional analysis of rice nucleotide pyrophosphatase

    Y Nanjo, N Ikarashi, H Oka, K Itoh, J Pozueta-Romero, T Mitsui

    PLANT AND CELL PHYSIOLOGY   46   S106 - S106   2005

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  • Characterization of rice nucleotide pyrophosphatase isoform

    Y Kondo, Y Nanjo, K Itoh, T Mitsui

    PLANT AND CELL PHYSIOLOGY   46   S106 - S106   2005

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  • Investigation of intracellular localization of rice alpha-amylase isoforms

    S Asatsuma, C Sawada, A Kitajima, T Mitsui

    PLANT AND CELL PHYSIOLOGY   46   S107 - S107   2005

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  • Involvement of alpha-amylase I-1 for starch metabolism in rice chloroplasts

    C Sawada, S Asatsuma, A Kitajima, K Itoh, T Mitsui

    PLANT AND CELL PHYSIOLOGY   46   S106 - S106   2005

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  • Mechanism of starch breakdown in rice chloroplasts

    T Mitsui, S Asatsuma, C Sawada, A Kitajima

    PLANT AND CELL PHYSIOLOGY   46   S1 - S1   2005

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  • Expression and biochemical analysis of rice kinesin

    N Umeki, Y Nakayama, K Kondo, T Mitsui, S Maruta

    PLANT AND CELL PHYSIOLOGY   46   S235 - S235   2005

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  • Proteomic analysis of rice Golgi complex: AtSYP-31/GFP-labeled Golgi membrenes

    S Hirose, T Asakura, M Sato, H Kaji, H Hori, T Mitsui

    PLANT AND CELL PHYSIOLOGY   46   S142 - S142   2005

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  • イネの花成におけるジベレリンシグナルの関与

    西村貫, 宮崎敬弘, ISLAM S. M. Shahinul, 三ツ井敏明, 三ツ井敏明, 横井修司, 島本功, 伊藤紀美子, 伊藤紀美子

    日本分子生物学会年会講演要旨集   28th   2005

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  • Regulatory mechanisms of α-amylase in germinating rice seeds.

    Shin-ichiro Mitsunaga, Mohammad A. Kashem, Masahiro Ohshima, Toshiaki Mitsui

    Current Topics in Plant Biology.   5   16 - 38   2004.12

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  • Comparative studies on biochemical analysis of mouse, C. elegans and rice kinesins.

    Y Nakayama, N Umeki, K Kondo, T Mitsui, S Maruta

    BIOPHYSICAL JOURNAL   86 ( 1 )   408A - 408A   2004.1

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  • Purification and cDNA cloning of rice nucleotide pyrophosphatase

    Y Nanjo, S Kurokawa, Y Kondo, K Itoh, J Potueta-Romero, T Mitsui

    PLANT AND CELL PHYSIOLOGY   45   S139 - S139   2004

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  • Analysis of alpha I-1 co-suppression line in rice

    S Asatsuma, C Sawada, M Ohshima, K Itoh, H Hori, T Mitsui

    PLANT AND CELL PHYSIOLOGY   45   S105 - S105   2004

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  • Proteome analysis of the regulation of rice seed germination

    T Asakura, T Nakaizumi, S Hirose, O Yato, H Hori, T Mitsui

    PLANT AND CELL PHYSIOLOGY   45   S148 - S148   2004

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  • The synthesis of α-amylase is induced by sulfuric acid-treatment in rice aleurone cells.

    MITSUNAGA S., KOBAYASHI M., OHSHIMA M., MITSUI T.

    4 ( 1 )   265 - 265   2002.3

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  • Interaction between Plasmodiophora brassicae and turnip callus resistant to clubroot disease - PAL activity and role of [Ca2+](cyt)

    H Takahashi, K Takita, T Mitsui, H Hori

    PLANT AND CELL PHYSIOLOGY   43   S237 - S237   2002

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  • Proteome analysis of proteins expressed in scutellar tissues of germinating rice seeds

    T Asakura, S Kimura, S Mikami, H Hori, T Mitsui

    PLANT AND CELL PHYSIOLOGY   43   S133 - S133   2002

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  • Two rice family of GRAS genes responsive to N-acetylchitooligosaccharide elicitor and phytoactive gibberellin

    R Day, M Koshioka, T Mitsui, M Ueguchi-Tanaka, M Matsuoka, N Shibuya, E Minami

    PLANT AND CELL PHYSIOLOGY   43   S155 - S155   2002

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  • Phosphate stimulates Ca2+ uptake and alpha-amylase II-4 secretion in rice cells.

    S Asatsuma, Y Nanjo, H Hori, T Mitsui

    PLANT AND CELL PHYSIOLOGY   43   S222 - S222   2002

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  • Identification and characterization of PNA-recognized glycoproteins associated with the Golgi complex in rice cells

    S Mikami, T Mitsui, H Hori

    PLANT AND CELL PHYSIOLOGY   43   S222 - S222   2002

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  • Bacillus thuringiensis specific to scarabaeid beetles: A review

    Hidetaka Hori, N. Selvamuthu Kumaraswami, Tohru Hayakawa, Toshiaki Mitsui

    Insect Science   7 ( 4 )   359 - 376   2000

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    DOI： 10.1111/j.1744-7917.2000.tb00235.x

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  • Characterization of Inositol 1,4,5-Trisphosphate-Receptor Like Glycoprotein in Rice Cells :

    Iwabuchi Satoru, Watanabe Satoko, Ito Kimiko, Hori Hidetaka, Mitsui Toshiaki

    Plant and cell physiology   41   s65   2000

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    Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=183786

  • Molecular Mechanism of Regulation of Rice Seed Germination

    MITSUI Toshiaki

    Nippon Nōgeikagaku Kaishi   73 ( 12 )   1273 - 1281   1999.12

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI： 10.1271/nogeikagaku1924.73.1273

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    Other Link： https://jlc.jst.go.jp/DN/JALC/00057907703?from=CiNii

  • Initial Reaction in the Infection of Turnip Calli with PlasmodioPhora brassicae

    Takahashi H., Muraoka S., Mitsui T., Hori H., Kiso A.

    Annals of the Phytopathological Society of Japan   65 ( 3 )   326 - 326   1999.6

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  • Activity and metabolism of 8',8',8'-trideuteroabscisic acid.

    Y Todoroki, S Nakano, N Hirai, T Mitsui, H Ohigashi

    PLANT PHYSIOLOGY   114 ( 3 )   797 - 797   1997.7

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  • イネ種子貯蔵タンパク質グルテリンの糖鎖について : 植物

    岸本 正, 三ツ井 敏明

    日本農藝化學會誌   69   74 - 74   1995.7

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  • 植物ゴルジ複合体における機能局在

    三ツ井敏明

    RADIOISOTOPES   43 ( 12 )   813 - 814   1994.12

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  • 植物培養細胞におけるβ-グルカンシンターゼ研究

    伊賀上郁夫, 栗林郁夫, 森田卓二, 三ツ井敏明

    日本農芸化学会誌   66 ( 12 )   1780 - 1783   1992.12

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  • BETA-GLUCAN SYNTHASES FROM CULTURED PLANT-CELLS

    IGAUE, I, KURIBAYASHI, I, T MORITA, T MITSUI

    NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY   66 ( 12 )   1780 - 1783   1992.12

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  • STRUCTURE AND FUNCTION OF GOLGI-COMPLEX IN CULTURED PLANT-CELLS

    T MITSUI, S KIMURA, IGAUE, I

    NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY   66 ( 12 )   1784 - 1787   1992.12

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  • 植物培養細胞におけるゴルジ複合体の構造と機能

    三ツ井敏明, 木村信, 伊賀上郁夫

    日本農芸化学会誌   66 ( 12 )   1784 - 1787   1992.12

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  • REGULATION OF SMOOTH-MUSCLE ACTOMYOSIN FUNCTION

    M IKEBE, T MITSUI, S MARUTA

    REGULATION OF SMOOTH MUSCLE CONTRACTION   304   25 - 36   1991

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  • PURIFICATION OF MYOSIN LIGHT CHAIN PHOSPHATASE FROM SMOOTH-MUSCLE ACTOMYOSIN

    M INAGAKI, T MITSUI, M IKEBE

    BIOPHYSICAL JOURNAL   57 ( 2 )   A145 - A145   1990.2

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  • イネα-アミラーゼの分泌

    三ツ井敏明

    化学と生物   27 ( 7 )   423 - 424   1989.7

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  • 分泌性酵素の生合成

    三ツ井敏明

    蛋白質核酸酵素 別冊30号   386 - 397   1987.7

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  • Biosynthesis and intracellular transport of α-amylase in germinating rice seeds.

    T. Akazawa, T. Mitsui

    Role of Biochemistry in Food and Energy production   223 - 228   1985.3

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Presentations

  • The hst1 gene promotes the growth performance of rice (Oryza sativa L.) genotypes under high temperature and drought stress

    E M. L. HORNAI, M. AYCAN, T. MITSUI

    The Japanese Society of Plant Physiologists  2023.3 

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    Event date： 2023.3

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  • Transgenerational salt plasticity improves the salinity-tolerance capacity of salt-sensitiveoffspring in rice (Oryza sativa L.)

    M. AYCAN, L. NAHAR, M. BASLAM, T. MITSUI

    The Japanese Society of Plant Physiologists  2023.3 

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  • Control of α-Amylase Expression Improves Rice Grain Quality under Elevated Temperature. Invited

    Toshiaki Mitsui

    Advances in Plants, Microbes and Agricultural Sciences  2023.3 

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  • Sdr4-k導入による酒造好適米'五百万石'の胚乳形成への影響

    箭内創, 氏原巧貴, 金澤伸矢, 熊谷夢都, 宮本託志, 大竹憲邦, 三ツ井敏明

    日本応用糖質科学会2024年（第73回）  2024.9 

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  • Enhancing Plant Growth and Productivity with Tokyo8 Biofertiliser in Sustainable Agriculture: A Study on Koshihikari Ric

    P. Mthiyane, T. Mitsui, N. Harada, K. Suzuki

    2024.9 

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  • Sdr4-k導入による酒造好適米“五百万石”の高温登熟耐性向上

    箭内創, 氏原巧貴, 金澤伸矢, 熊谷夢都, 宮本託志, 大竹憲邦, 三ツ井敏明

    日本作物学会第257回講演会  2024.3 

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  • Sdr4-kを用いた酒造好適米‘五百万石’の心白形成制

    箭内創, 氏原巧貴, 金澤伸矢, 熊谷夢都, 宮本託志, 大竹憲邦, 三ツ井敏明

    応用糖質科学会東日本支部ミニシンポジウム  2023.10 

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  • イネの澱粉代謝制御研究－応用分子細胞生物学から新新種開発へ Invited

    三ツ井敏明

    第63回新潟生化学懇話会  2023.7 

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  • Proteomics of Starch Metabolism in Rice Under High Temperature and Elevated CO2 Conditions Invited International conference

    MITSUI Toshiaki

    13th MALAYSIA INTERNATIONAL GENETICS CONGRESS  2019.11 

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    Venue：Malaysia  

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  • 遺伝子発現マーカーによるイネの白未熟粒発生予測技術の開発

    白矢武士, 太田沙由理, 三ツ井敏明, 土田徹

    第56回北陸作物・育種学会  2019.7 

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    Venue：福井市AOSSA  

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  • Insights into the mechanisms involved in the improvement of yields and quality of Rice exposed to volatile compounds emitted by phytopathogens under climate change. International conference

    Marouane BASLAM, Kimiko ITOH, Kaneko KENTARO, Edurne BAROJA-FERNANDEZ, Francisco JOSE MUNOZ, Mohammad-Reza HAJIREZAEI, Karel DOLEZAL, Javier POZUETA-ROMERO, Toshiaki MITSUI

    XXIII Meeting of the Spanish Society of Plant Physiology/XVI Spanish Portuguese Congress of Plant Physiology  2019.6 

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    Venue：Pamplona,Spain  

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  • イネにおける澱粉代謝関連酵素の応用分子細胞生物学的研究 Invited

    三ツ井 敏明

    平成30年度 日本応用糖質科学会北海道支部 シンポジウム  2019.1  日本応用糖質科学会北海道支部

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    Venue：札幌市  

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  • Proteomic and Glycomic Characterization of Rice Chalky Grains Produced under Moderate and High-Temperature Conditions in Field System International conference

    Toshiaki MITSUI, Kentaro KANEKO, Maiko SASAKI, Nanako KURIBAYASHI, Hiromu SUZUKI, Yukiko SASUGA, Takeshi SHIRAYA, Takuya INOMATA, Kimiko ITOH, Marouane BASLAM

    Joint symposium of the 8th International Agriculture Congress 2018 And 6th International symposium for food & Agriculture 2018  2018.11 

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    Venue：Malaysia  

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  • Phytopathogens: A Good Opportunity to Improve Rice Culture Under Changing Environmental Conditions International conference

    Marouane BASLAM, Kimiko ITOH, Edurne BAROJA-FERN?NDEZ, Mohammad-Reza HAJIREZAEI, Karel DOLEZAL, Javier POZUETA-ROMERO, Toshiaki MITSUI

    Joint symposium of the 8th International Agriculture Congress 2018 And 6th International symposium for food & Agriculture 2019  2018.11 

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    Venue：Malaysia  

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  • EIG CONCERT-Japan POISE/IRUEC プロジェクト紹介

    三ツ井敏明, 伊藤紀美子, Baslam Marouane, 高松壮, 猪俣拓也

    サイエンスアゴラ2018（JST）  2018.11 

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    Venue：東京都テレコムビル  

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  • Roles of autophagy in endosperm development during rice seed maturation International conference

    KAAB International Symposium 2018  2018.9 

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    Venue：Niigata university  

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  • Phytopathogens: A Good Opportunity to Improve Rice Culture Under Changing Environmental Conditions International conference

    Shigeru HANAMATA, Yuri SERA, Shingo SAKAMOTO, Seijiro ONO, Kentaro KANEKO, Yuudai MITSUI, Tomoko KOYANO, Naoko FUJITA, Takehiro MASUMURA, Hikaru SAJI, Ken-ichi NONOMURA, Nobutaka MITSUDA, Toshiaki MITSUI, Takamitsu KURUSU, Kazuyuki KUCHITSU

    KAAB International Symposium 2018  2018.9 

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    Venue：Niigata university  

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  • Rapid improvement of salinity tolerance in an elite rice cultivar through an efficient SNP marker-aided backcrossing coupled with speed breeding technique International conference

    Md Masud RANA, Takeshi TAKAMATSU, Marouane BASLAM, Kentaro KANEKO, Kimiko ITOH, Naoki HARADA, Toshie SUGIYAMA, Takayuki OHNISHI, Tetsu KINOSHITA, Akira ABE, Hiroki TAKAGI, Toshiaki MITSUI

    KAAB International Symposium 2018  2018.9 

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    Venue：Niigata university  

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  • Optimized method of extracting rice chloroplast DNA for high-quality plastome resequencing and de novo assembly International conference

    Takeshi TAKAMATSU, Marouane BASLAM, Takuya INOMATA, Kazusato OIKAWA, Kimiko ITOH, Takayuki OHNISHI, Tetsu KINOSHITA, Toshiaki MITSUI

    KAAB International Symposium 2018  2018.9 

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    Venue：Niigata university  

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  • イネにおける澱粉代謝関連酵素の応用分子細胞生物学的研究（ Invited

    三ツ井 敏明

    平成30年度大会（第67回）日本応用糖質科学会全員集会、授賞式  2018.9  日本応用糖質科学会

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    Venue：秋田県立大学  

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  • Optimized Method of Extracting Rice Chloroplast DNA for High-Quality Plastome Resequencing and de Novo Assembly International conference

    Takeshi TAKAMATSU, Marouane BASLAM, Takuya INOMATA, Kazusato OIKAWA, Kimiko ITOH, Takayuki OHNISHI, Tetsu KINOSHITA, Toshiaki MITSUI

    ISRFG  2018.9 

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    Venue：東京農業大学  

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  • 酒米の高温登熱被害によるメタボローム変化

    太田奈々恵, 椎名将平, 金古堅太郎, 花城勲, 三ツ井敏明

    第36回日本植物細胞分子生物学会  2018.8 

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    Venue：金沢商工会議所会館  

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  • 炊飯米物性の変化に関係する細胞壁合成遺伝子の同定

    白矢武士, 太田沙由理, 三ツ井敏明

    第８回北陸植物学会  2018.6 

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    Venue：富山大学  

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  • 白未熟粒発生による米の食味低下に関係するタンパク質の同定

    白矢武士, 太田沙由理, 三ツ井敏明, 佐藤徹, 東聡志

    第59回日本植物生理学会年会  2018.3 

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    Venue：札幌コンベンションセンター  

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  • Microbial volatiles modulate rapid responses in Arabidopsis through thiol oxidation of cysteines as revealed by quantitative sitespecific redox proteomics

    Marouane BASLAM, Kinia AMEZTOY-DEL AMO, Kentaro KANEKO, Francisco Jos?, MUNOZ, Angela Maria, SANCHEZ-LOPEZ, Edurne BAROJA-FERNANDEZ, Toshiaki MITSUI, Javier POZUETA-ROMERO

    第59回日本植物生理学会年会  2018.3 

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    Venue：札幌コンベンションセンター  

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  • 玄米外観品質における高CO2の影響

    若松 和, 齋藤 誠志, 猪俣 拓也, 金古 堅太郎, Marouane BASLAM, 三ツ井 敏明

    日本農芸化学会2018年度大会  2018.3 

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    Venue：名城大学  

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  • H2O2プライミングはイネの高温不稔と品質低下を改善する

    流石 有希子, 三井 悠大, 金古 堅太郎, Marouane BASLAM, 三ツ井 敏明

    日本農芸化学会2018年度大会  2018.3 

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    Venue：名城大学  

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  • 膜交通を利用したプラスチドタンパク質蓄積技術の開発：TMN1のプラスチドターゲティング機構

    三ッ井 敏明, 河田 圭介, 新井 琢也, 古賀 彩, 高松 壮, 猪俣 拓也, 金古 堅太郎, バスラム マロワン

    日本農芸化学会2018年度大会  2018.3 

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    Venue：名城大学  

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  • イネ由来α−アミラーゼの触媒反応機構に対する構造的洞察

    落合 秋人, 菅井 寛, 田中 孝明, 谷口 正之, 三ツ井 敏明

    日本農芸化学会2018年度大会  2018.3 

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    Venue：名城大学  

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  • 高温登熟による炊飯米物性の変化に関係する細胞壁合成遺伝子の同定 International conference

    白矢武士, 太田沙由理, 三ツ井敏明, 佐藤 徹, 東 聡志

    ConBio2017  2017.12 

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    Venue：神戸ポートアイランド  

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  • EIG CONCERT-Japan POISE/IRUEC プロジェクト紹介 International conference

    三ツ井敏明, 伊藤紀美子, Baslam Marouane, 高松壮, 猪俣拓也

    サイエンスアゴラ2017（JST）  2017.11 

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    Venue：テレコムセンタービル  

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  • Hydrogen peroxide priming induces high temperature tolerance in seedling growth and heading stages International conference

    Yukiko SASUGA, Yudai MITSUI, Kentaro KANEKO, Takeshi TAKAMATSU, Marouane BASLAM, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Rice transmembrane nine 1 is involved in membrane traffic through secretory pathway to plastids International conference

    Keisuke KAWATA, Kazusato OIKAWA, Aya KOGA, Takeshi TAKAMATSU, Kentaro KANEKO

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Smart breeding: Pyramiding genes for salt-, heat- and drought-tolerant rice cultivar using Marker Assisted Backcrossing (MABC) International conference

    Md masud RANA, Takeshi TAKAMATSU, Takuya INOMATA, Kentaro KANEKO, Marouane BASLAM, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • CONCERT Japan Project POISE&IRUEC: to find solutions ensuring stable, high quality and sustainable food production against changing global climate International conference

    Kimiko ITOH, Marouane BASLAM, Kentaro KANEKO, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Rice transmembrane nine protein 1 is involved in membrane trafficking to plastid through secretory pathway International conference

    Toshiaki MITSUI, Kazusato OIKAWA, Yuki NAKAYAMA, Aya KITAJIMA-KOGA, Takeshi TAKAMATSU, Takuya INOMATA, Namiko ITO, Reo TANAKA, Marouane BASLAM, Kentaro KANEKO, Leszek A. KLECZKOWSKI

    KAAB International Symposium 2017  2017.9 

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  • Phytopathogens: A good opportunity to improve crop yields and quality under changing environmental conditions (POISE) International conference

    Marouane BASLAM, Kimiko ITOH, Edurne BAROJA-FERNÁNDEZ, Mohammad-Reza HAJIREZAEI, Karel DOLEZAL, Lukas SPÍCHAL, Nuria DE DIEGO, Javier POZUETA-ROMERO, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Towards a multi-approach study focused on improving resource use efficiency in cereals under climate change (IRUEC) International conference

    Marouane BASLAM, José Luis ARAUS, Bertr, GAKIÈRE, Eckart, PRIESACK, Iker ARANJUELO, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Functional analysis of late embryogenesis abundant (LEA) proteins in heat stress-induced chalky grain of rice International conference

    Ayuka KATOH, Yuuki SATOH, Kentaro KANEKO, Ignacio EZQUER, Marouane BASLAM, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Effect of Elevated CO2 (ECO2) on appearance quality of rice grains International conference

    Nodoka WAKAMATSU, Masashi SAITO, Takuya INOMATA, Kentaro KANEKO, Marouane BASLAM, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Function and Molecular Structure of Oryza sativa α-Amylase Ⅰ-1 International conference

    Hirokazu OGIHARA, Aya KOGA-KITAJIMA, Kentaro KANEKO, Akihito OCHIAI, Masayuki TANIGUCHI, Ken HANZAWA, Syunji NATSUKA, Kimiko ITOH, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Evidence for the occurrence of starch degradation and cycling in illuminated arabidopsis leaves International conference

    Marouane BASLAM, Edurne BAROJA-FERNÁNDEZ, Adriana RICARTE-BERMEJO, Ángela maría, SÁNCHEZ LÓPEZ, Iker ARANJUELO, Abdellatif BAHAJI, Francisco José Muñoz, Goizeder ALMAGRO, Pablo PUJOL, Regina GALARZA, Toshiaki MITSUI, Pilar TEIXIDOR, Javier POZUETA-ROMERO

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Microbial volatiles modulate rapid responses in arabidopsis through thiol oxidation of cysteines as revealed by quantitative site-specific redox proteomics International conference

    Kinia AMEZTOY DEL AMO, Marouane BASLAM, Kentaro KANEKO, Francisco José MUÑOZ, Ángela maría, SÁNCHEZ-LÓPEZ, Abdellatif BAHAJI, Goizeder ALMAGRO, Edurne BAROJA-FERNÁNDEZ, Toshiaki MITSUI, Javier POZUETA-ROMERO

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Influence of nitrogen nutrition on photosynthetic redox homeostasis and energy balance in rice plants exposed to elevated CO2 condition International conference

    Marouane BASLAM, Kentaro KANEKO, Kazusato OIKAWA, Takuya INOMATA, Takeshi TAKAMATSU, Md Masud RANA, Iker ARANJUELO, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Identification of molecular processing events and degradome pattern in Oryza sativa subsp.Japonica at International conference

    Riho AKATSUKA, Marouane BASLAM, Javier POZUETA-ROMERO, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • Redox sensing proteome analysis in hydrogen peroxide priming treatment International conference

    Yudai MITSUI, Yukiko SASUGA, Kentaro KANEKO, Marouane BASLAM, Toshiaki MITSUI

    KAAB International Symposium 2017  2017.9 

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    Venue：Niigata Univ.  

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  • アブシジン酸（ABA）の中茎伸長作用における生理機構－胚乳養分の分解と可溶性糖類の消長－

    渡邊肇, 上村一真, 安達祐介, 板谷越重人, 高松壮, 三ツ井敏明

    日本作物学会第244回講演会  2017.9 

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    Venue：岐阜大学  

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  • イネ由来α-アミラーゼの糖結合部位に対する機能解析

    山田大貴, 落合秋人, 荻原寛和, 田中孝明, 三ツ井敏明, 谷口正之

    第69回生物工学会年次大会  2017.9 

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    Venue：早稲田大学  

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  • イネの種子登熟におけるオートファジーの役割

    瀬良ゆり, 花俣繁, 坂本真吾, 小野聖二郎, 三井悠大, 金古堅太郎, 北畑信隆, 三ツ井敏明, 野々村賢一, 光田展隆, 来須孝光, 朽津和幸

    日本植物学会第８１回大会  2017.9 

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    Venue：東京理科大野田キャンパス  

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  • 米粒白濁化メカニズムの解明－澱粉粒構造と澱粉鎖長分布の解析－

    金古堅太郎, 椎名将平, 太田奈々恵, 徳永成就, 野見山奨, 花城勲, 三ツ井敏明

    日本応用糖質学会平成29年度大会  2017.9 

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    Venue：日本大学湘南キャンパス  

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  • Comprehensive bioinformatic approach for analyzing and detecting human missing proteins

    Amr ELGUOSHY, Yoshitoshi HIRAO, Bo XU, Suguru SAITO, Ali F.QUADERY, Keiko YAMAMOTO, Toshiaki MITSUI, Tadashi YAMAMOTO

    日本プロテオーム学会2017年大会  2017.7 

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    Venue：ホテル阪急エキスポパーク  

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  • Comprehensive bioinformatic approach for analyzing and detecting human missing proteins

    Amr ELGUOSHY, Yoshitoshi HIRAO, Bo XU, Suguru SAITO, Ali F.QUADERY, Keiko YAMAMOTO, Toshiaki MITSUI, Tadashi YAMAMOTO

    日本プロテオーム学会2017年大会  2017.7 

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    Venue：ホテル阪急エキスポパーク  

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  • Microbial volátiles modulate rapid responses in Arabidopsis through thiol oxidation of cysteines as revealed by quantitative site-specific redox proteomics International conference

    Kinia AMEZTOY DEL AMO, Marouane BASLAM, Francisco José MUÑOZ, Ángela María, SÁNCHEZ-LÓPEZ, Abdellatif BAHAJI, Goizeder ALMAGRO, Edurne BAROJA-FERNÁNDEZ, Toshiaki MITSUI, Javier POZUETA-ROMERO

    FV2017  2017.6 

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    Venue：Barcelona (Spain)  

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  • Rice transmembrane nine protein 1 is involved in membrane trafficking through secretory pathway to plastid Invited International conference

    Toshiaki MITSUI, Kazusato OIKAWA, Aya KITAJIMA-KOGA, Takeshi TAKAMATSU, Keisuke KAWATA, Kentaro KANEKO, Marouane BASLAM

    Plant Biology 2017  2017.6 

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    Venue：Hawaii Convention Center, Honolulu  

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  • 高温登熟により発生した白未熟米粒の定量的プロテオーム解析

    白矢武士, 三ツ井敏明

    北陸植物学会  2017.6 

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  • Rice α-Amylase Involved in Grain Chalking under Heat Stress Invited International conference

    Toshiaki MITSUI, Kentaro KANEKO, Hirokazu OGIHARA, Yukiko SASUGA, Shouhei SHIINA, Yudai MITSUI, Marouane BASLAM, Takeshi TAKAMATSU, Takuya INOMATA

    Green for Good IV: Biotechnology of Plant Products  2017.6 

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    Venue：palacký Univ., Olomouc, Czech Republic  

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  • Effects of nitrogen nutrition on chloroplast homeostasis and photosynthesis in rice plants exposed to Elevated CO2 condition International conference

    Marouane BASLAM, Kentaro KANEKO, Kazusato OIKAWA, Takuya INOMATA, Iker ARANJUELO, Toshiaki MITSUI

    Photosynthesis Workshop  2017.5 

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    Venue：Pamplona (Spain).  

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  • The role of protein system in NPP1 mutant under high temperature and elevated CO2 condition

    Marouane BASLAM, Takuya INOMATA, Kentaro Kaneko, Nodoka WAKAMATSU, Toshiaki MITSUI

    日本農芸化学会2017年度大会  2017.3 

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    Venue：京都女子大学  

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  • 玄米外観品質における高CO2の影響

    若松和, 涌井翔太郎, 猪俣拓也, 金古堅太郎, Baslam Marouane, 三ツ井敏明

    日本農芸化学会2017年度大会  2017.3 

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    Venue：京都女子大学  

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  • 酒米における白濁化メカニズムの解明：走査型電子顕微鏡による解析

    椎名将平, 栗林なな子, 金古堅太郎, 三ツ井敏明

    日本農芸化学会2017年度大会  2017.3 

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  • 高温登熟乳白部位特異的プロテオーム解析

    金古堅太郎, 佐々木麻衣子, 佐藤友紀, 椎名将平, 三ツ井敏明

    日本農芸化学会2017年度大会  2017.3 

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    Venue：京都女子大学  

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  • イネの生殖・プログラム細胞死・代謝制御におけるオートファジーの役割

    朽津和幸, 瀬良ゆり, 澤田隼平, 陶文紀, 小野聖二郎, 花俣繁, 坂本真吾, 光田展隆, 三ツ井敏明

    第58回日本植物生理学会年会  2017.3 

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    Venue：鹿児島大学  

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  • 高温登熟による米の食感の低下に関係する遺伝子の同定

    白矢武士, 太田沙由理, 三ツ井 敏明, 佐藤徹, 東聡志

    第58回日本植物生理学会年会  2017.3 

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    Venue：鹿児島大学  

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  • Effects of Nitrogen nutrition on photosynthetic redox homeostasis in rice plants exposed to Elevated CO2 condition International conference

    Marouane Baslam, Kentaro Kaneko, Kazusato Oikawa, Takuya Inomata, Iker Aranjuelo, Toshiaki Mitsui

    第58回日本植物生理学会年会  2017.3 

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    Venue：鹿児島大学  

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  • 植物細胞におけるTMN1 のタンパク質局在及び分泌経路機能の解析

    河田圭介, 及川和聡, 古賀彩, BaslamMarouane, 高松壮, 猪俣拓也, 金古堅太郎, 伊藤紀美子

    第58回日本植物生理学会年会  2017.3 

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    Venue：鹿児島大学  

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  • 米粒の白濁化メカニズム Invited

    三ツ井敏明

    東京理科大学セミナー  2016.12 

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    Venue：東京理科大学  

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  • 高温登熟による米の食味・食感の低下に関係する遺伝子の同定

    白矢武士, 太田沙由理, 三ツ井敏明, 佐藤徹, 東聡志

    第39回日本分子生物学会年会  2016.11 

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    Venue：パシフィコ横浜  

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  • Life Science Seminar in Univ. Milan Invited International conference

    Toshiaki Mitsui

    The Cell Biological Study of Rice alpha-Amylase ～ Plastid-targeting of glycoproteins through secretory pathway ～  2016.11 

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    Venue：Università degli Studi di Milano, Milan  

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  • Molecular mechanism of Golgi-to-Plastid traffic in rice cell Invited International conference

    Toshiaki Mitsui

    Cutting Edge Seminar  2016.11 

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    Venue：KBC lecture hall, Umeå  

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  • Salinity Tolerance of Japonica Rice at Seedling Stage International conference

    Rana MD MASUD, Takeshi TAKAMATSU, Takuya INOMATA, Yukiko SASUGA, Marouane BASLAM, Kentaro KANEKO, Kazusato OIKAWA, Kimiko ITOH, Toshiaki MITSUI

    KAAB International Symposium 2016  2016.9  KAAB

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  • eduction of rice grain quality grown under elevated CO2 concentration International conference

    Nodoka WAKAMATSU, Shotaro WAKUI, Takuya INOMATA, Kentaro KANEKO, Toshiaki MITSUI

    KAAB International Symposium 2016  2016.9  KAAB

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    Venue：Niigata Univ.  

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  • Function and Molecular Structure of Oryza sativa α-Amylase I-1 International conference

    Hirokazu OGIHARA, Aya KOGA-KITAJIMA, Kentaro KANEKO, Akihito OCHIAI, Masayuki TANIGUCHI, Ken HANZAWA, Syunji NATSUKA, Kimiko ITO, Toshiaki MITSUI

    KAAB International Symposium 2016  2016.9  KAAB

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    Venue：Niigata Univ.  

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  • Hydrogen peroxide priming induces high temperature tolerance of rice plant International conference

    Yukiko SASUGA, Hiromu SUZUKI, Kentaro KANEKO, Toshiaki MITSUI

    KAAB International Symposium 2016  2016.9  KAAB

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    Venue：Niigata Univ.  

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  • Functional analysis of TMN1 on transporting of Amylase I-1 to plastids via secretory pathway International conference

    Kazusato OIKAWA, Yuki NAKAYAMA, Namiko ITO, Takuya INOMATA, Aya KOGA, Takeshi TAKAMATSU, Baslam MAROUANE, Kentarou KANEKO, Kimiko ITO, Toshiaki MITSUI

    KAAB International Symposium 2016  2016.9  KAAB

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    Venue：Niigata Univ.  

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  • Imaging analysis of rice α-Amylase transport to plastids through the secretory pathway International conference

    Keisuke KAWATA, Aya KOGA, Kazusato OIKAWA, Tomoko TANIUCHI, Takeshi TAKAMATSU

    KAAB International Symposium 2016  2016.9  KAAB

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    Venue：Niigata Univ.  

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  • Whole-genome sequencing reveals a large deletion on chromosome 6 in a rice wx mutant International conference

    Nao NOMURA, Takeshi TAKAMATSU, Hidekazu SHIMIZU, Toshiaki MITSUI, Kimiko ITOH

    KAAB International Symposium 2016  2016.9  KAAB

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    Venue：Niigata Univ.  

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  • Effect of Compost Supply and Mycorrhizal Inoculation on Growth of Date Palm (Phoenix dactylifera L.) Plants International conference

    Abdelilah MEDDICH, Toshiaki MITSUI, Marouane BASLAM

    KAAB International Symposium 2016  2016.9  KAAB

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    Venue：Niigata Univ.  

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  • Phytopathogens: a good opportunity to improve crop yields and quality; a case study of RICE International conference

    Marouane BASLAM, Kentaro KANEKO, Kazusato OIKAWA, Takeshi TAKAMATSU, Javier POZUETA-ROMERO, Toshiaki MITSUI

    KAAB International Symposium 2016  2016.9  KAAB

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    Venue：Niigata Univ.  

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  • Effects of Nitrogen nutrition on photosynthetic redox homeostasis in rice plants exposed to Elevated CO2 condition International conference

    Marouane BASLAM, Kentaro KANEKO, Kazusato OIKAWA, Takuya INOMATA, Iker ARANJUELO, Toshiaki MITSUI

    KAAB International Symposium 2016  2016.9  KAAB

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    Venue：Niigata Univ.  

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  • イネα-アミラーゼⅠ-1の構造と機能に関する研究

    荻原寛和, 古賀彩, 金古堅太郎, 落合秋人, 谷口正之, 半澤健, 長束俊治, 伊藤紀美子, 三ツ井敏明

    日本応用糖質科学会平成28年度大会（第６５回）  2016.9 

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    Venue：福山大学  

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  • 新潟大学における農業分野等でのバイオサイエンス研究 Invited

    三ツ井敏明

    バイオサイエンスシンポジウムin新潟  2016.8  新潟県

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    Venue：ホテルオークラ  

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  • 高温登熟による米の食味・食感の変化に関係する遺伝子の同定

    白矢武士, 太田沙由理, 佐藤徹, 東聡志, 三ツ井敏明

    北陸植物学会 平成28年度大会  2016.6 

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    Venue：富山大学  

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  • 低濃度過酸化水素プライミングはイネに高温ストレス耐性を付与する

    流石有希子, 鈴木浩武, 栗林なな子, 金古堅太郎, 三ツ井敏明

    第57回新潟生化学懇話会（平成28年度）  2016.6 

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    Venue：新潟大学医歯学総合病院新潟医療人育成センター  

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  • 植物オルガネラのダイナミクスを視る（接着～分解）

    及川和聡, 細川陽一郎, 西村幹夫, 三ツ井敏明

    第57回新潟生化学懇話会（平成28年度）  2016.6 

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    Venue：新潟大学医歯学総合病院新潟医療人育成センター  

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  • 高CO2ストレスによる玄米外観品質低下メカニズムの解析

    若松和, 涌井翔太郎, 猪俣拓也, 金古堅太郎, 三ツ井敏明

    第57回新潟生化学懇話会（平成28年度）  2016.6 

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    Venue：新潟大学医歯学総合病院新潟医療人育成センター  

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  • 植物の生存戦略に果たすオルガネラ相互作用と分解機構の役割

    及川和聡, 真野昌二, 三ツ井敏明, 細川陽一郎, 西村幹夫

    第68回日本細胞生物学会大会  2016.6 

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    Venue：京都テルサ  

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  • 高温登熟乳白と酒米心白の生化学的比較解析による白濁化メカニズムの解明

    栗林なな子, 佐々木麻衣子, 鈴木浩武, 流石有希子, 金古堅太郎, 三ツ井敏明

    日本農芸化学会2016年度大会  2016.3 

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    Venue：札幌  

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  • イネにおいて過酸化水素プライミングは環境ストレスの抵抗性を付加する

    流石有希子, 鈴木浩武, 栗林なな子, 金古堅太郎, 佐々木麻衣子, 丸山達也, 森太紀, 白矢武士, 三ツ井敏明

    日本農芸化学会2016年度大会  2016.3 

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    Venue：札幌  

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  • 次世代シーケンス解析に向けた高純度のイネ葉緑体DNA精製法

    高松壮, 大西孝幸, 猪俣拓也, 及川和聡, 木下哲, 三ツ井敏明

    日本農芸化学会2016年度大会  2016.3 

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    Venue：札幌  

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  • 葉緑体包膜に局在するOsLACS9の解析

    谷内智子, 濱田侑紀, 高松壮, 石山隆一, 及川和聡, 古賀彩, 三ツ井敏明

    第57回日本植物生理学会年会  2016.3 

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    Venue：岩手大学  

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  • 分泌経路を介して葉緑体タンパク質輸送に関するTMPsの解析 International conference

    及川和聡, 猪俣拓也, 中山勇希, 伊東七実子, 石山隆一, 谷内智子, 高松壮, 古賀彩, 金古堅太郎, 三ツ井敏明

    第57回日本植物生理学会年会  2016.3 

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    Venue：岩手大学  

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  • コシヒカリの高温登熟種子におけるタンパク質及び遺伝子の発現解析 International conference

    白矢武士, 佐藤徹, 東聡志, 三ツ井敏明

    第57回日本植物生理学会年会  2016.3 

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    Venue：岩手大学  

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  • 分泌経路を介してプラスチドへ輸送されるイネTMN1の解析

    及川和聡, 伊東七実子, 中山勇希, 石山隆一, 金古堅太郎, 古賀-北嶋彩, 谷内智子, 高松壮, 三ツ井敏明

    BMB2015 (第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会)  2015.12 

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    Venue：神戸  

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  • 稲の高温登熟障害発生を診断できる遺伝子発現マーカーの開発

    白矢武士, 佐藤徹, 東聡志, 三ツ井敏明

    BMB2015 (第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会)  2015.12 

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    Venue：神戸  

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  • 分泌経路を介してプラスチドへ輸送されるイネTMN1の解析

    及川和聡, 伊東七実子, 中山勇希, 石山隆一, 金古堅太郎, 古賀-北嶋彩, 谷内智子, 高松壮, 三ツ井敏明

    BMB2015 (第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会)  2015.12 

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    Venue：神戸  

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  • 水稲種子休眠とその浸種条件が種子発芽及びα-アミラーゼの発現に及ぼす影響

    板谷越 重人, 水澤 誠一, 澁川 洋, 三ツ井 敏明

    第36回種子生理生化学研究会年会  2015.11 

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    Venue：新潟  

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  • イネ澱粉代謝の応用分子細胞生物学～新たな酒米開発に向けて～（特別講演） International conference

    三ツ井敏明

    醸造試験場報告会  2015.10 

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    Venue：新潟  

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  • Functional analysis of TMN1 in transporting of rice α-Amylase to plastids via secretory pathway International conference

    Kazusato OIKAWA, Yuki NAKAYAMA, Namiko ITO, Ryuichi ISHIYAMA, Tomoko TANIUCHI, Aya KITAJIMA-KOGA, Takeshi TAKAMATSU, Kentro KANEKO, Kimiko ITO, Toshiaki MITSUI

    KAAB International Symposium 2015  2015.9 

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    Venue：新潟  

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  • Production and characterization of novel glycogen-based polymer ~Joint research by Public University of Navarra and Niigata University International conference

    Mamiko FUKUSHIMA, Goizeder ALMAGRO,Isao HANASHIRO,Javier, POZUETA ROMERO

    KAAB International Symposium 2015  2015.9 

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    Venue：Niigata  

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  • Clarification of chalking mechanism of rice grains caused by normal and high temperature during grain filling International conference

    Nanako KURIBAYASHI, Maiko SASAKI, Hiromu SUZUKI, Yukiko SASUGA, Kentaro KANEKO, Toshiaki MITSUI

    KAAB International Symposium 2015 Poster Session  2015.9 

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    Venue：Niigata  

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  • Comparative proteomics of rice endosperm proteins from seven cultivars, differences in physicochemical properties of the starches International conference

    Masataka NIHEI, Mari WATANABE, Kentaro KANEKO, Kimiko ITOH, Toshiaki MITSUI

    KAAB International Symposium 2015 Poster Session  2015.9 

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    Venue：Niigata  

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  • Protein and gene expression analysis of grains under high temperature stress in rice variety ‘Koshihikari’ International conference

    Takeshi SHIRAYA, Toshiaki MITSUI

    KAAB International Symposium 2015 Poster Session  2015.9 

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    Venue：Niigata  

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  • Plastid targeting of alpha-amylaseⅠ-1 in rice cells International conference

    Hirokazu OGIHARA, Aya KITAJIMA-KOGA, Takashi TAKAMATSU, Kazusato OIKAWA, Kentaro KANEKO, Toshiaki MITSUI

    KAAB International Symposium 2015 Poster Session  2015.9 

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    Venue：Niigata  

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  • Functional analysis of OsLACS9 at chloroplasts envelope membrane International conference

    Tomoko TANIUCHI, Yuki HAMADA, Takeshi TAKAMATSU, Namiko ITO, Ryuichi ISHIYAMA, Aya KOGA, Kazusato OIKAWA, Toshiaki MITSUI

    KAAB International Symposium 2015 Poster Session  2015.9 

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    Venue：Niigata  

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  • N-glycomic and microscopic subcellular localization analyses of NPP1, 2 and 6 strongly indicate that trans-Golgi compartments participate in the Golgi-to-plastid traffic of nucleotide pyrophosphatase/phosphodiesterases in rice International conference

    Kentaro KANEKO, Takeshi TAKAMATSU, Takuya INOMATA, Kazusato OIKAWA, Kimiko ITOH, Javier POZUETA-ROMERO, Toshiaki MITSUI

    KAAB International Symposium 2015 Poster Session  2015.9 

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    Venue：Niigata  

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  • Suppressive effects of low seed-soaking temperatures on germination of long-term-stored rice seeds International conference

    Shigeto ITAYAGOSHI, Seiichi MIZUSAWA, Osamu KAWAKAMI, Hiroshi SHIBUKAWA, Takeshi TAKAMATSU, Maiko SASAKI, Kentaro KANEKO, Toshiaki MITSUI

    KAAB International Symposium 2015 Poster Session  2015.9 

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    Venue：Niigata  

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  • 質量分析器を用いた澱粉物性の異なるイネ品種間のプロテオーム解析

    伊藤紀美子, 渡邊茉莉, 金古堅太郎, 二瓶正崇, 三ツ井敏明

    応用糖質科学会  2015.9  応用糖質科学会

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    Venue：奈良  

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  • 登熟期間のCO２濃度がコメ外観品質に与える影響

    猪俣拓也, 金古堅太郎, 涌井翔太郎, 若松和, 三ツ井敏明

    第4回応用糖質フレッシュシンポジウム  2015.9 

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    Venue：大阪  

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  • 光合成を効率化するオルガネラ相互作用

    及川和聡, 真野昌二, 三ツ井敏明, 細川陽一郎, 西村幹夫

    応用糖質科学会フレッシュシンポジウム  2015.9 

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    Venue：大阪  

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  • 高CO2濃度による玄米白濁化に関する研究

    涌井翔太郎, 若松 和, 猪俣拓也, 金古堅太郎, 三ツ井敏明

    第4回応用糖質フレッシュシンポジウム  2015.9 

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    Venue：大阪  

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  • Volatile compounds emitted by the plant pathogen Alternaria alternata enhance photosynthesis and promote growth of Arabidopsis plants by increasing cytokinin production

    Baslam Marouane, Sánchez-López Angela, De Diego Nuria, Li Jun, Baroja-Fernández Edurne, Bahaji Abdellatif, Ricarte-Bermejo Adriana, Sesma Maite Teresa, Muñoz Francisco José, Novák Ondřej, Spíchal Lukas, Doležal Karel, Pozueta-Romero Javier

    第４回応用糖質フレッシュシンポジウム  2015.9 

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    Venue：大阪  

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  • 高温登熟耐性イネ品種NU1号の遺伝子解析

    鈴木浩武, 森 太紀, 丸山達也, 佐々木麻衣子, 栗林なな子, 流石有希子, 金古堅太郎, 高松 壮, 三ツ井敏明

    第４回応用糖質フレッシュシンポジウム  2015.9 

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    Venue：大阪  

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  • コメ外観品質に及ぼす登熟期高CO2濃度の影響

    猪俣拓也, 金古堅太郎, 涌井翔太郎, 若松 和, 三ツ井敏明

    第４回応用糖質フレッシュシンポジウム  2015.9 

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    Venue：大阪  

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  • イネのオルガネラゲノムのリシーケンス

    大西孝幸, 高松壮, 及川和聡, 猪俣拓也, 田中啓介, 古海弘康, 倉田のり, 小林久人, 三ツ井敏明, 木下哲

    日本育種学会第128回講演会  2015.9 

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    Venue：新潟  

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  • 形態変化を伴ったオルガネラ間相互作用の解析～オルガネラ間接着力測定の試み～

    及川和聡, 真野昌二, 近藤真紀, 坂本亘, 三ツ井敏明, 飯野敬短, 細川陽一郎, 西村幹夫

    日本植物学会第79回大会  2015.9 

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    Venue：新潟  

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  • Plastid targeting of alpha-amylaseⅠ-1 in rice cells International conference

    Hirokazu OGIHARA, Aya KITAJIMA-KOGA, Takashi TAKAMATSU, Kazusato OIKAWA, Kentaro KANEKO, Toshiaki MITSUI

    The 5th Asian Conference on Green Technology in Agriculture  2015.7 

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    Venue：Chiang Mai , Thailand  

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  • Clarification of chalking mechanism of rice grains caused by normal and high temperature during grain filling International conference

    Nanako KURIBAYASHI, Maiko SASAKI, Hiromu SUZUKI, Yukiko SASUGA, Kentaro KANEKO, Toshiaki MITSUI

    The 5th Asian Conference on Green Technology in Agriculture  2015.7 

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    Venue：Chiang Mai, Thailand  

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  • Functional analysis of OsLACS9 at chloroplasts envelope membrane International conference

    Tomoko TANIUCHI, Yuki HAMADA, Takeshi TAKAMATSU, Namiko ITO, Ryuichi ISHIYAMA, Aya KOGA, Kazusato OIKAWA, Toshiaki MITSUI

    The 5th Asian Conference on Green Technology in Agriculture  2015.7 

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    Venue：Chiang Mai, Thailand  

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  • イネにおいてヌクレオチドピロホスファターゼ／ホスホジエステラーゼの欠損がデンプンの蓄積と光合成に影響する

    増井貴広, 猪俣拓也, 涌井翔太郎, 金古堅太郎, 三ツ井敏明

    日本農芸化学会2015年度大会  2015.3 

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    Venue：Okayama  

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  • プラスチド局在スーパーオキシドジムスターゼはイネ登熟期における高温被害米の発生を低減する

    金古堅太郎, 佐々木麻衣子, 白矢武士, 高松壮, 及川和聡, 古川豊和, 三ツ井敏明

    日本農芸化学会2015年度大会  2015.3 

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  • 葉緑体包膜に局在するOsLACS9の機能解析

    谷内智子, 濱田侑紀, 高松壮, 伊東七実子, 石山隆一, 古賀彩, 及川和聡, 三ツ井敏明

    第56回日本植物生理学会年会  2015.3 

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  • ゴルジ体‐葉緑体間タンパク質輸送に関与するイネ膜タンパク質TMPの解析

    及川和聡, 伊東七実子, 中山勇希, 石山隆一, 金古堅太郎, 古賀彩, 谷内智子, 高松壮, 三ツ井敏明

    第56回日本植物生理学会年会  2015.3 

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    Venue：Tokyo  

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  • 高温・高CO2条件においてヌクレオチドピロホスファターゼ／ホスホジエステラーゼの欠損がデンプンの蓄積と光合成に影響する

    増井貴広, 猪俣拓也, 涌井翔太郎, 金古堅太郎, 三ツ井敏明

    第56回日本植物生理学会年会  2015.3 

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  • 水稲種子休眠とその浸種条件が種子発芽及びα‐アミラーゼの発現に及ぼす影響

    水稲種子休眠とその浸種条件が種子発芽及び, ‐アミラーゼの発現に及ぼす影響

    第35回種子生理生化  2014.11 

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    Venue：新潟  

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  • イネSUMOパラログに結合するタンパク質の同定

    伊藤紀美子, 串岡拓也, 安宅浩, 今井なつき, 金古堅太郎, 三ツ井敏明

    第35回種子生理生化  2014.11 

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    Venue：新潟  

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  • 遺伝子発現マーカーによる稲高温登熟障害発生診断技術の開発

    白矢武士, 佐藤徹, 佐々木麻衣子, 三ツ井敏明

    日本水稲品質・食味研究会 第6回講演会  2014.11 

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    Venue：京都府立大学  

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  • 高温耐性品種開発に向けた遺伝子レベルでの挑戦 Invited

    三ツ井敏明

    にいがた夢農業・人づくり事業共通講座シンポジウム  2014.10 

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    Venue：新潟だ学  

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  • イネ由来α-アミラーゼの立体構造とその熱安定性に関与する構造要因の解析

    落合秋人, 菅井寛, 伊東孝祐, 内海利男, 田中孝明, 谷口正之, 三ツ井敏明

    第８７回日本生化学会大会  2014.10 

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    Venue：京都  

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  • イネ由来α-アミラーゼの立体構造とその熱安定性に関与する構造要因の解析

    落合秋人, 菅井寛, 伊東孝祐, 内海利男, 田中孝明, 谷口正之, 三ツ井敏明

    第８７回日本生化学会大会  2014.10 

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    Venue：京都  

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  • Chloroplast envelope membrane protein, Oryza sativa LACS9, is targeted through unknown vesicle traffic to chloroplast International conference

    Takeshi Takamatsu, Yuki Hamada, Tomoko Taniuchi, Namiko Ito, Toshiaki Mitsui

    KAAB International Symposium 2014  2014.9 

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    Venue：Niigata  

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  • Light-Dependent Organelles Communication in Photosynthetic Tissues International conference

    Kazusato OIKAWA, Maki KONDO, Shoji MANO, Wataru SAKAMOTO, Takanori IINO, Yoichiroh HOSOKAWA, Toshiaki MITSUI, Mikio NISHIMURA

    KAAB International Symposium 2014  2014.9 

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    Venue：Niigata  

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  • Clarification of generating mechanism of chalky rice grains caused by high temperature during grain filling International conference

    Nanako KURIBAYASHI, Kentaro KANEKO, Takeshi SHIRAYA, Maiko SASAKI, Toshiaki MITSUI

    KAAB International Symposium 2014  2014.9 

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    Venue：Niigata  

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  • A proteomic analysis of female Nipponia nippon International conference

    Takuya INOMATA, Kentaro KANEKO, Takahiro MASUI, Kimiko ITOH, Javier POZUETA-ROMERO, Toshiaki MITSUI

    KAAB International Symposium 2014  2014.9 

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    Venue：Niigata  

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  • Study of subcellular Localization of rice SUMO3 International conference

    Mamiko FUKUSHIMA, Natsuki NOGUCHI, Takuya KUSHIOKA, Kentaro KANEKO, Attia KOTB, Toshiaki MITSUI, Kimiko ITOH

    KAAB International Symposium 2014  2014.9 

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    Venue：Niigata  

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  • Knockout mutation of NPP1 gene enhances starch accumulation and growth in rice seedlings under high temperature and high CO2 concentration conditions

    akuya INOMATA, Kentaro KANEKO, Takahiro MASUI, Kimiko ITOH, Javier POZUETA-ROMERO, Toshiaki MITSUI

    KAAB International Symposium 2014  2014.9 

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  • HPLC-MS/MS analyses of ADPglucose content in Arabidopsis leaves reveal that the starch-deficient aps1 and pgm mutants accumulate wild type ADPglucose content International conference

    Abdellatif BAHAJI, Edurne BAROJA-FERNÀNDEZ, Jun LI, Francisco José MUÑOZ, Ángela María, SÀNCHEZ-LÒPEZ, Goizeder ALMAGRO, Manuel MONTERO, Miroslav OVECKA, Pablo PUJOL, Regina GALARZA, Kentaro KANEKO, Kazusato OIKAWA, Kaede WADA, Toshiaki MITSUI, Javier POZUETA-ROMERO

    KAAB International Symposium 2014  2014.9 

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    Venue：Niigata  

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  • Structure-based functional analysis of α-amylase from rice (Oryza sativa) International conference

    Hiroshi SUGAI, Akihito OCHIAI, Masayuki TANIGUCHI, Toshiaki MITSUI

    KAAB International Symposium 2014  2014.9 

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    Venue：Niigata  

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  • Knockout mutation of Nucleotide Pyrophosphatase/Phosphodiesterase 1 gene stimulates accumulation of starch and photosynthesis International conference

    Takahiro MASUI, Takuya INOMATA, Syotaro WAKUI, Kentaro KANEKO, Toshiaki MITSUI

    KAAB International Symposium 2014  2014.9 

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    Venue：Niigata  

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  • イネのヌクレオチドピロホスファターゼ/ホスホジエステラーゼ1（NPP1）の欠損が糖代謝に及ぼす影響

    増井貴広, 猪俣拓也, 涌井翔太郎, 金古堅太郎, 三ツ井敏明

    応用糖質学会会平成26 年度大会（第63 回）  2014.9 

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    Venue：niigata  

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  • イネのヌクレオチドピロホスファターゼ/ホスホジエステラーゼ1（NPP1）の欠損が糖代謝に及ぼす影響

    増井貴広, 猪俣拓也, 涌井翔太郎, 金古堅太郎, 三ツ井敏明

    応用糖質学会会平成26 年度大会（第63 回  2014.9 

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    Venue：niigata  

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  • NPP1 は高温・高CO2条件下においてイネ芽生えの澱粉蓄積および成長制御に関与する

    猪俣拓也, 金古堅太郎, 増井貴広, 伊藤紀美子, ポズエタ-ロメロ ハビア, 三ツ井敏明

    第３回応用糖質フレッシュシンポジウム  2014.9 

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    Venue：新潟  

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  • イネのプラスチドタンパク質新奇輸送経路に関する研究

    谷内智子, 濱田侑紀, 髙松壮, 伊東七実子, 石山隆一, 古賀彩, 及川和総, 三ツ井敏明

    第３回応用糖質フレッシュシンポジウム  2014.9 

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    Venue：新潟  

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  • NPP1 の欠損変異が光合成と糖代謝に及ぼす影響 Invited

    増井貴広, 猪俣拓也, 涌井翔太郎, 金古堅太郎, 三ツ井敏明

    第３回応用糖質フレッシュシンポジウム  2014.9 

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    Venue：新潟  

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  • イネ由来α-アミラーゼの立体構造とその多機能性の解析

    落合秋人, 谷口正之, 三ツ井敏明

    第３回応用糖質フレッシュシンポジウム  2014.9 

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    Venue：新潟  

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  • イネ由来α-アミラーゼAmyI-1のＸ線結晶構造解析

    落合秋人, 菅井寛, 原田計, 伊東孝祐, 内海利男, 田中孝明, 谷口正之, 三ツ井敏明

    第６６回日本生物工学会 大会  2014.9 

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    Venue：札幌コンベンションセンター  

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  • 稲の高温登熟障害発生を診断できる遺伝子発現マーカーの開発 優秀発表賞（口頭発表部門）

    白矢武士, 三ツ井敏明

    第238回日本作物学会講演会  2014.9 

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    Venue：愛媛大学城北キャンパス  

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  • イネα-アミラーゼと米品質 Invited

    三ツ井敏明

    第38回酒米懇談会  2014.9 

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    Venue：東京  

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  • Establishment of selection marker-free rice-based oral cholera toxin B-subunit vaccine and characterization of location and structure of transgene by using whole genome resequencing analysis International conference

    Yoshikazu YUKI, Mio MEJIMA, Koji KASHIMA, Masaharu KURODA, Toshiaki MITSUI, Hiroshi KIYON

    International association for plant biotechnology congress 2014  2014.8 

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    Venue：Melbourne, Australia  

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  • イネ高温登熟耐性に関与するゴルジ体／プラスチド型スーパーオキシドジスムターゼ

    三ツ井敏明, 白矢武士, 佐々木麻衣子, 丸山達也, 髙松壮, 及川和聡, 金古堅太郎

    日本プロテオーム学会2014年会  2014.7 

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    Venue：つくば国際会議場  

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  • Suppression of α-amylase genes improves quality of rice grain ripened under high temperature

    Hiromoto YAMAKAWA, Makoto HAKATA, Masaharu KURODA, Tomomi MIYASHITA, Takeshi YAMAGUCHI, Mikiko KOJIMA, Hitoshi SAKAKIBARA, Toshiaki MITSUI

    Plant Biology 2014  2014.7 

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    Venue：Portland  

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  • A glycoprotein nucleotide pyrophosphatase/phosphodiesterase 1 exerts a negative effect on starch accumulation in rice

    Takuya INOMATA, Takahiro MASUI, Takeshi TAKAMATSU, Kentaro KANEKO, Javier POZUETA-ROMERO, Toshiaki MITSUI

    Plant Biology 2014  2014.7 

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    Venue：Portland  

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  • 暑さに負けないイネ品種の開発 Invited

    三ツ井敏明

    平成26年度 市民大学前期講座 にいがた市民大学「激変する自然環境下での農業～バイテクによる植物改変からの挑戦～」  2014.6 

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  • イネ葉緑体包膜タンパク質LACS9の輸送機構の研究（ポスター発表優秀賞受賞）

    高松壮, 伊東七実子, 谷内智子, 古賀彩, 濵田侑紀, 三ツ井敏明

    第55回新潟生化学懇話会（平成26年度  2014.6 

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    Venue：長岡技術科学大学  

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  • NPP1 は高温・高CO2条件下において

    猪俣拓也, 金古堅太郎, 増井貴広, 伊藤紀美子, ポズエタ-ロメロ ハビア, 三ツ井敏明

    第55回新潟生化学懇話会（平成26年度）  2014.6 

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    Venue：長岡技術科学大学  

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  • イネSUMO3の細胞内局在性制御の研究

    福島真美子, 野口夏希, 伊藤紀美子, 及川和聡, 三ツ井敏明

    イネSUMO3の細胞内局在性制御の研究（ポスター）  2014.6 

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    Venue：長岡技術科学大学  

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  • NPP1は高温・高CO２環境下における成長およびデンプン蓄積を負に制御する

    金古堅太郎, 猪俣拓也, 増井貴広, 伊藤紀美子, ハビアポズエタ-ロメロ, 三ツ井敏明

    日本農芸化学会2014年度大会  2014.3 

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    Venue：明治大学  

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  • 米型経口コレラワクチンMarker Free MucoRice-CTBのイネゲノム導入部位と導入配列について

    目島未央, 鹿島光司, 黒田昌治, 竹内夏実, 黒河志保, 福山賀子, 清野宏, 三ツ井敏明, 幸義和

    日本農芸化学会2014年度大会  2014.3 

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    Venue：明治大学  

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  • Golgi/plastid-type superoxide dismutase involves in high temperature stress tolerance during grain filling of rice

    Maiko SASAKI, Takeshi SHIRAYA, Tatsuya MARUYAMA, Toshiaki MITSUI

    第55回日本植物生理学会年会  2014.3 

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    Venue：富山大学  

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  • NPP1は高温・高CO2条件下においてイネ芽生えの澱粉蓄積および成長制御に関与する

    猪俣拓也, 金古堅太郎, 増井貴広, 伊藤紀美子, ポズエタ-ロメロ ハビア, 三ツ井敏明

    第55回日本植物生理学会年会  2014.3 

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    Venue：富山大学  

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  • フェムト秒レーザーを用いた光依存的オルガネラ間接着力評価 Invited International conference

    及川和聡, 真野昌二, 林誠, 山田健志, 近藤真紀, 東正一, 渡辺正勝, 三ツ井敏明, 飯野敬矩, 重政彰徳, 細川陽一郎, 西村幹夫

    第55回日本植物生理学会年会  2014.3 

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    Venue：富山大学  

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  • Characteristics of various rice grains stressed at high temperature

    Kazumi TSUTSUI, C. Nishikawa, Toshiaki MITSUI, Kentaro KANEKO, Kazutosi HAYASE, Katsuyoshi NISHINARI

    第23回日本MRS年次大会  2013.12 

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    Venue：横浜  

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  • イネα-アミラーゼの機能発現制御と米品質 Invited

    三ツ井敏明

    植物化学調節学会第48回大会  2013.10 

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    Venue：新潟大学  

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  • 乳白米粒の澱粉・プロテオーム解析

    佐々木麻衣子, 金古堅太郎, 丸山達也, 白矢武士, 澤田隆行, 三ツ井敏明

    日本応用糖質科学会平成25年度大会（第62回）・シンポジウム  2013.9 

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    Venue：鹿児島  

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  • Acceleration of germination of rice seeds by soaking with red onion International conference

    Sumiko NAKAMURA, Kentaro KANEKO, Toshiaki MITSUI, Ken’ichi OHTSUBO

    12th HUPO World Congress 2013  2013.9 

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    Venue：Yokohama  

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  • Comprehensive identification of OsSUMO binding proteins in rice exposed to high temperature stress condition International conference

    Takuya KUSHIOKA, Hiroshi ATAKA, Attia KOTB, Kentaro KANEKO, Toshiaki MITSUI, Kimiko ITOH

    12th HUPO World Congress 2013  2013.9 

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    Venue：Yokohama  

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  • Starch glycomic and proteomic aspects of grain chalkness International conference

    KANEKO, Maiko SASAKI, Tatsuya MARUYAMA

    12th HUPO World Congress 2013  2013.9 

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  • Mutation of nucleotide pyrophosphatase/phosphodiesterase 1 stimulates accumulation of starch in rice seedlings under high CO2 concentration International conference

    akahiro MASUI, Takuya INOMATA, Maiko SASAKI, Kentaro KANEKO, Toshiaki MITSUI

    12th HUPO World Congress 2013  2013.9 

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    Venue：Yokohama  

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  • A proteomic analysis of the japanese Crested Ibis International conference

    Toshiaki MITSUI, Kentaro KANEKO, Yoshinori KANEKO, Hideaki YAMASHIRO, Takahisa YAMADA, Toshie SUGIYAMA, Maiko SASAKI, Chizuo NISHIDA, Yoichi MATSUDA, Mitsuru OYANAGI

    12th HUPO World Congress 2013  2013.9 

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  • Proteomic analysis on eleven Crested Ibis’ tissues using shotgun

    Mitsuru OYANAGI, Kentaro KANEKO, Yoshinori KANEKO, Hideaki YAMASHIRO, Takahisa YAMADA, Toshie SUGIYAMA, Maiko SASAKI, Chizuko NISHIDA, Yoichi MATSUDA, Toshiaki MITSUI

    第86回日本生化学会大会  2013.9 

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  • 高温被害米の澱粉の糊化特性

    筒井和美, 斎藤由佳, 三ツ井敏明, 西成勝好

    第60回記念大会 日本食品科学工学会  2013.8 

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    Venue：東京  

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  • イネのヌクレオチドピロホスファターゼ／ホスホジエステラーゼ1（NPP1)の欠損変異が気孔開閉に及ぼす影響（

    増井 貴広, 猪俣拓也, 金古堅太郎, 三ツ井敏明

    第54回新潟生化学懇話会（平成25年度）  2013.7 

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    Venue：新潟大学脳研究所  

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  • イネSUMOパラログの細胞内局在性と基質の解明

    安宅浩, 串岡拓也, 野口夏希, Kotb Attia, 金古堅太郎, 三ツ井敏明, 伊藤紀美子

    第54回新潟生化学懇話会（平成25年度）  2013.7 

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    Venue：新潟大学脳研究所  

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  • 高温被害米の物理化学的特性の解明

    筒井和美, 金古堅太郎, 花城 勲, 西成勝好, 三ツ井敏明

    日本調理科学会 東海・北陸支部 第10回研究発表会  2013.7 

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    Venue：三重大学  

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  • デンプン代謝改変による乳白粒低減イネ開発の試み‐合成か分解か、それが問題だ

    山川博幹, 羽方誠, 黒田昌治, 宮下朋美, 山口武志, 小嶋美紀子, 榊原均, 三ツ井敏明

    日本作物学会第235回講演会  2013.3 

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    Venue：明治大学  

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  • 水稲高温登熟に関するプロテオーム解析 Invited

    三ツ井敏明, 金古堅太郎, 丸山達也, 佐々木麻衣子, 白矢武士, 山川博幹

    日本作物学会第235回講演会  2013.3 

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    Venue：明治大学  

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  • 貧栄養ストレスに対する応答の異なる品種を用いたアサガオのストレス応答花成制御の解析

    貧栄養ストレスに対する応答の異なる品種を用いたアサガオのストレス応答花成制御の解析

    第54回日本植物生理学会  2013.3 

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    Venue：岡山大学  

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  • イネSUMO／E2パラログの細胞核内における局在性の研究（

    野口夏希, 三ツ井敏明, 一色正之, 伊藤紀美子

    第54回日本植物生理学会  2013.3 

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    Venue：岡山大学  

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  • α-アミラーゼの発現制御は高温登熟で発生するイネの乳白粒を低減させる

    羽方誠, 黒田昌治, 宮下朋美, 山口武志, 小嶋美紀子, 榊原均, 三ツ井敏明, 山川博幹

    第54回日本植物生理学会  2013.3 

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    Venue：岡山大学  

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  • シソの弱光ストレス応答花成を制御する代謝系

    三木聖美, 和田 楓, 金古堅太郎, 三ツ井敏明, 竹能清俊

    第54回日本植物生理学会  2013.3 

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    Venue：岡山大学  

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  • 塩ストレスにより活性カルボニル修飾を受けるタンパク質の同定

    真野純一, 永田光曜, 岡村星多郎, 白矢武士, 三ツ井敏明

    第54回日本植物生理学会  2013.3 

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    Venue：岡山大学  

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  • Diverse localization of rice membrane protein TMN1

    Ryuichi ISHIYAMA, Namiko ITO, Tomoko TANIUCHI, Yuki NAKAYAMA, Kaede WADA, Kentaro KANEKO, Toshiaki MITSUI

    The 54th Annual Meeting of the Japanese Society of Plant Physiologists  2013.2 

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    Venue：Okayama University  

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  • SUMO化によるMYB様転写因子の安定化と局在性の制御 International conference

    野口 夏希, 五十嵐雄太, 岡田理子, 三ツ井敏明, 伊藤紀美子

    日本農芸化学会2012年度（平成24年度）大会 関東支部  2012.10 

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  • 高温登熟により発生した白未熟米粒の定量的プロテオーム解析 International conference

    白矢 武士, 丸山 達也, 佐々木 麻衣子, 金古堅太郎, 三ツ井 敏明

    日本水稲品質・食味研究学会 第4回講演会  2012.10 

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    Venue：旭川  

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  • 高温ストレスにより発生した白未熟米粒の定量プロテオーム解析 International conference

    丸山達也, 佐々木麻衣子, 白矢武士, 金古堅太郎, 三ツ井敏明

    日本応用糖質学会平成24年度大会（第61回）  2012.9 

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  • α-アミラーゼの抑制はイネの高温登熟障害を軽減する International conference

    羽方誠, 黒田昌治, 三ツ井敏明, 山川博幹

    日本応用糖質学会平成24年度大会（第61回）  2012.9 

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  • 高温登熟障害米粒のプロテオーム解析

    三ツ井敏明, 佐々木麻衣子, 丸山達也, 白矢武士, 金古堅太郎

    平成24年度 日本プロテオーム学会年会  2012.7 

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  • イネゴルジ体のプロテオーム解析 International conference

    石山隆一, 伊東七実子, 中山勇希, 三ツ井敏明

    平成24年度 日本プロテオーム学会年会  2012.7 

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    Venue：日本科学未来館  

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  • イネnpp1変異体の高CO2・高温における定量的プロテオミクス解析 International conference

    金古堅太郎, 白矢武士, 猪俣拓也, 甲州努, 増井貴広, 北島‐古賀綾, 三ツ井敏明

    平成24年度 日本プロテオーム学会年会  2012.7 

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    Venue：日本科学未来館  

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  • 高温ストレスにより発生した白未熟米粒の定量的プロテオミクス解析

    佐々木麻衣子, 丸山達也, 白矢武士, 金古堅太郎, 三ツ井敏明

    平成24年度 日本プロテオーム学会年会  2012.7 

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    Venue：日本科学未来館  

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  • イネの高温登熟障害が発生するメカニズムを解明

    三ツ井敏明, 山川博幹

    新農業展開ゲノムプロジェクトシンポジウム  2012.7 

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    Venue：東京大学伊藤国際学術研究センター伊藤謝恩ホール  

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  • イネデンプン集積抑制酵素変異体の高CO2、高温におけるプロテオミクス解析

    金古堅太郎, 白矢武士, 甲州努, 猪俣拓也, 三ツ井敏明

    第53 回新潟生化学懇話会（平成24 年度）  2012.6 

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    Venue：新潟  

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  • イネにおけるヌクレオチドピロホスファターゼ／ホスホジエステラーゼのプラスチド局在化に関する研究

    梅澤幸歩, 金古堅太郎, 甲州努, 石本卓也, 伊藤紀美子, 林八寿子, 豊岡公徳, 三ツ井敏明

    第53回 新潟生化学懇話会  2012.6 

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    Venue：新潟大学  

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  • イネにおけるゴルジ体のプロテオーム解析～Trans Membrane Nine Protein1の細胞内分布

    石山隆一, 伊東七実子, 中山勇希, 三ツ井敏明

    第53回 新潟生化学懇話  2012.6 

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    Venue：新潟大学  

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  • アサガオの貧栄養ストレス応答花成におけるサリチル酸とPnFT2の役割 International conference

    山田瑞樹, 和田楓, 古塩綾, 金古堅太郎, 白矢武士, 星野敦, 酒井達也, 三ツ井敏明, 加藤朗, 竹能清俊

    北陸植物学会 平成24年度大会  2012.6 

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  • 高温耐性コシヒカリの開発

    三ツ井敏明

    新潟大学新技術説明会  2012.6 

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    Venue：JST東京別館ホール  

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  • イネヌクレオチドピロホスファターゼ／ホスホジエステラーゼのプラスチド局在に関する研究

    梅澤幸歩, 金古堅太郎, 甲州努, 石本卓也, 伊藤紀美子, 林八寿子, 豊岡公徳, 三ツ井敏明

    日本農芸化学会2012  2012.3 

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    Venue：京都  

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  • イネ由来抗菌タンパク質の同定とヒト病原菌に対する抗菌活性の評価

    落合秋人, 原田 計, 柴田一駿, 三ツ井敏明, 田中孝明, 谷口正之

    日本農芸化学会2012  2012.3 

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    Venue：京都  

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  • イネゴルジ体プロテオーム解析：Endomembrane protein 70

    中山勇希, 石山隆一, 伊藤七実子, 三ツ井敏明

    第５３回日本植物生理学会  2012.3 

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    Venue：京都  

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  • イネのマンガン型スーパーオキシドジスムターゼ（MSD1）は高温登熟耐性に機能する

    白矢武士, 森 太紀, 大久保英奈, 丸山達也, 金古堅太郎, 三ツ井敏明

    第５３回日本植物生理学会  2012.3 

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  • Regulation of Starch Metabolism in Rice-The Discovery of Golgi-to-plastid Traffic

    Toshiaki MITSUI

    2012 Niigata Graduate Research Forum  2012.1 

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    Venue：Toki Messe, Niigata  

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  • 稲澱粉代謝関連酵素の細胞分子生物学と高温耐性稲の開発戦略

    三ツ井敏明

    H２３年 日本水稲品質・食味研究会 第３回講演会in 新潟  2011.11 

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    Venue：新潟大学大学院自然科学研究科棟大会議室  

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  • Current status of plant proteomics research in Japan International conference

    Toshiaki Mitsui

    Frontiers in Agriculture Proteome Research -Contribution of proteomics technology in agricultural science-  2011.11 

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    Venue：つくば国際会議場エポカル  

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  • Structure and function of nucleotide pyrophosphatase/ phosphodiesterases localized in plastids of rice

    Kentaro KANEKO, Toshiaki MITSUI, Tsutomu KOSHU, Sachiho UMEZAWA, Aya KITAJIMA-KOGA, Takeshi SHIRAYA, Kazuko HIROSE, Maho AMANO, Shin-Ichiro NISHIMURA, Javier POZUETA-ROMERO

    Frontiers in Agriculture Proteome Research -Contribution of proteomics technology in agricultural science-  2011.11 

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    Venue：つくば国際会議場エポカル  

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  • Involvement of Endomembrane-type Superoxide Dismutase in High Temperature Stress Tolerance during Grain Filling of Rice International conference

    Toshiaki MITSUI, Takeshi SHIRAYA, Taiki MORI, Kentaro KANEKO

    Frontiers in Agriculture Proteome Research -Contribution of proteomics technology in agricultural science-  2011.11 

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  • Proteomic Analysis of Ripening Grains of Rice under High Temperature Stress

    Toshiaki Mitsui

    Thai-Niigata Seminar by JENESYS Program 2009(JSPS Invitation Program for East Asian Young Researchers)  2011.10 

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    Venue：Large Meeting Room in Graduate School of Science and Technology  

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  • プラスチド糖タンパク質の局在化メカニズムと生理機能解析

    三ツ井敏明

    第78回北海道大学Plant Science Seminar  2011.9 

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  • イネにおける新規でんぷん集積関連酵素NPPの生理機能解析

    甲州努, 金古堅太郎, 梅澤幸歩, 三ツ井敏明

    日本応用糖質科学会 平成23年度大会(第60回) 第19回糖質関連酵素化学シンポジウム  2011.9  日本応用糖質科学会

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    Venue：北海道大学札幌キャンパス  

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  • 各種乳白粒の物理化学的特性

    筒井和美, 廣神里奈, 金古堅太郎, 西成勝好, 三ツ井敏明

    日本応用糖質科学会平成23年度大会(第60回) 第19回糖質関連酵素化学シンポジウム  2011.9  日本応用糖質科学会

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    Venue：北海道大学札幌キャンパス  

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  • イネヌクレオチドピロホスファターゼ/ホスホジエステラーゼのプラスチド局在化機構に関する研究

    梅澤幸歩, 金古堅太郎, 甲州努, 石本卓也, 伊藤 紀美子, 林八寿子, 豊岡公徳, 三ツ井敏明

    第84回日本生化学会大会  2011.9 

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  • Golgi-to-Plastid traffic in higher plant cells

    Toshiaki Mitsui, Aya Kitajima-Koga, Kentaro Kaneko, Takeshi Shiraya, Akihiko Nakano, Kiminori Toyooka, Ken Matsuoka

    第84回日本生化学会大会  2011.9 

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  • イネにおけるヌクレオチドピロホスファターゼ/ホスホジエステラーゼの生理機能解析

    甲州努, 金古堅太郎, 梅澤幸歩, 三ツ井敏明

    第84回日本生化学会大会  2011.9 

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  • イネの高温登熟耐性におけるマンガン型スーパーオキシドジスムターゼ（MSD1）の機能解析

    白矢武士, 森太紀, 大久保英奈, 丸山達也, 金古堅太郎, 三ツ井敏明

    第84回日本生化学会大会  2011.9 

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  • イネに存在する植物特異的キネシンの生化学的特徴付け

    梅津のぞみ, 近藤和典, 三ツ井敏明, 丸田晋策

    第84回日本生化学会大会  2011.9 

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  • プラスチド局在糖タンパク質NPPの糖鎖構造解析

    金古堅太郎, 甲州努, 梅澤幸歩, 石本卓也, 天野麻穂, 西村紳一郎, 三ツ井敏明

    第84回日本生化学会大会  2011.9 

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  • 定量的プロテオーム解析によるイネの高温登熟耐性メカニズムの解明

    白矢武士, 森太紀, 大久保英奈, 丸山達也, 金古堅太郎, 三ツ井敏明

    平成23年度 日本プロテオーム学会年会  2011.7 

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  • プラスチド局在糖タンパク質NPPのグライコミクス解析

    金古堅太郎, 甲州努, 梅澤幸歩, 石本卓也, 天野麻穂, 西村伸一郎, 三ツ井敏明

    平成23年度 日本プロテオーム学会年会  2011.7 

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  • イネα-アミラーゼI-1過剰発現細胞におけるゴルジ体のプロテオーム解析

    石山隆一, 中山勇希, 高山和俊, 朝倉剛, 三ツ井敏明

    平成23年度 日本プロテオーム学会年会  2011.7 

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  • イネの高温登熟耐性に関係する遺伝子の探索および機能解析

    白矢武士, 森太紀, 大久保英奈, 丸山達也, 金古堅太郎, 三ツ井敏明

    平成23年度 日本プロテオーム学会年会  2011.7 

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  • イネプラスチド局在型ヌクレオチドピロホスファターゼ／ホスホジエステラーゼのN-グライコーム解析：機能糖タンパク質がゴルジ装置の後期区画からプラスチドに輸送される

    三ツ井敏明, 金古堅太郎, 甲州努, 梅澤幸歩, 古賀彩, 白矢武士, 広瀬和子, 天野麻穂, 西村伸一郎, ポズエタ-ロメロ ハビア

    平成23年度 日本プロテオーム学会年会  2011.7 

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  • イネゴルジ体のプロテオーム解析

    石山隆一, 中山勇希, 高山和俊, 朝倉剛, 三ツ井敏明

    平成23年度 北陸植物学会大会  2011.6 

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  • ヌクレオチドピロホスファターゼ/ホスホジエステラーゼがイネにおけるデンプン代謝に与える影響

    甲州努, 金古堅太郎, 梅澤幸歩, 三ツ井敏明

    平成23年度 北陸植物学会大会  2011.6 

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  • イネ糖タンパク質NPPのプラスチド局在化機構に関する研究

    梅澤幸歩, 金古堅太郎, 甲州努, 石本卓也, 伊藤紀美子, 林八寿子, 豊岡公徳, 三ツ井敏明

    平成23年度 北陸植物学会大会  2011.6 

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  • イネα-アミラーゼI-1プラスチド局在化シグナルの解析 International conference

    伊藤多絵子, 古賀彩, 岡田久夫, 濵田侑紀, 金古堅太郎, 三ツ井敏明

    平成23年度 北陸植物学会大会  2011.6 

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  • 高温登熟性の異なるイネ品種の遺伝子発現特性解析(II)

    森太紀, 白矢武士, 大久保英奈, 丸山達也, 三ツ井敏明

    平成23年度 日本農芸化学会大会  2011.3 

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  • イネ高温登熟耐性に関係する新規遺伝子の探索

    白矢武士, 森太紀, 大久保英奈, 丸山達也, 金古堅太郎, 古賀(北島)彩, 濱田侑紀, 水谷理絵, 甲州努, 中山勇希, 石山隆一, 伊藤多絵子, 梅澤幸歩, 木村泉, 石本卓也, 猪俣拓也, 小泉拓真, 三ツ井敏明

    平成23年度 第52回日本植物生理学会年会  2011.3 

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  • 高温ストレスに強いイネを開発する

    三ツ井敏明

    新農業展開ゲノムプロジェクト・富山シンポジウム 2010 「ここまでできた！お米の研究最前線」  2010.12 

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  • The characterization of the novel rice kinesin K23 at kinesin-7 subfamiliy

    Nozomi Umezu, Nobue Hanzawa, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta

    第83回日本生化学会大会 第33回日本分子生物学会年会合同大会  2010.12 

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  • Function analysis of Os ROS1a by homologous recombination-promoted gene targeting.

    「Akemi Ono」「Katsushi Yamaguchi」「Yasuyo Hisatomi」「Toshiaki Mitsui」「Rie Terada」「Shigeru Iida」

    第83回日本生化学会大会 第33回日本分子生物学会年会合同大会  2010.12 

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  • イネの高温登熟耐性遺伝子の探索

    森 太紀, 白矢武士, 大久保英奈, 三ツ井敏明

    第83回日本生化学会大会 第33回日本分子生物学会年会合同大会  2010.12 

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  • イネの高温登熟耐性遺伝子の探索

    森太紀, 白矢武士, 大久保英奈, 三ツ井敏明

    第83回日本生化学会大会 第33回日本分子生物学会年会合同大会  2010.12 

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  • 高温に強い稲の開発

    三ツ井敏明

    第1回新潟大学・刈羽村先端農業バイオ研究センターフォーラム  2010.11 

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  • Search of Genes Involved in Hgh Temperature Tolerance during Grain Filling of Rice International conference

    Taiki MORI, Takeshi SHIRAYA, Ena OKUBO, Toshiaki MITSUI

    2nd International Symposium on Frontier in Agriculture Proteome Research –Contribution of proteomics technology in agricultural science-  2010.11 

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  • Search of genes involved in high temperature tolerance during grain filling of rice. International conference

    「Taiki Mori」「Takeshi Shiraya」「Ena Okubo」「Toshiaki Mitsui」

    2nd International Symposium on Frontier in Agriculture Proteome Research  2010.11 

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  • イネ糖タンパク質NPPプラスチド局在機構に関する研究

    梅沢幸歩, 金古堅太郎, 甲州努, 北嶋(古賀)彩」, 柳田愛, 山田智恵, 豊岡公徳, 伊藤紀美子, 三ツ井敏明

    植物電子顕微鏡若手ワークショップ  2010.11 

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  • 乳心白粒の物理化学的特性に関する研究

    筒井和美, 原田亜由美, 金古堅太郎, 西成勝好, 三ツ井敏明

    日本応用糖質科学会平成22年度大会(第59回) 第18回糖質関連酵素化学シンポジウム  2010.9 

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  • イネα-アミラーゼ I -1プラスチド局在化シグナルの解析

    水谷理絵, 岡田久夫, 浜田侑紀, 大久保英奈, 伊藤多絵子, 伊藤紀美子, 三ツ井敏明

    日本応用糖質科学会平成22年度大会(第59回) 第18回糖質関連酵素化学シンポジウム  2010.9 

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  • N-glycome in rice plastidial nucleotide pyrophosphatase/phosphodiesterase glycoproteins International conference

    Toshiaki Mitsui

    Plant Biology 2010  2010.7 

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  • イネの高温登熟障害が発生するメカニズムを解明

    三ツ井敏明, 山川博幹

    農林水産技術会議  2010.7 

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  • イネゴルジ体プロテオーム解析 ： ゴルジ体 ー プラスチド間輸送活性化によって誘導されるゴルジ体タンパク質

    中山勇希, 石山隆一, 高山和俊, 朝倉剛, 三ツ井敏明

    日本植物学会北陸支部 平成22年度大会  2010.6 

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  • イネにおける新規デンプン集積抑制酵素・ヌクレオチドピロホスファターゼ/ホスホジエステラーゼの生理機能の解析

    甲州努, 金古堅太郎, 梅澤幸歩, 柳田愛, 伊藤紀美子, 三ツ井敏明

    日本植物学会北陸支部 平成2年度大会  2010.6 

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    Venue：富山大学  

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  • plastid targeting of NPP glycoprotein from the golgi appalatus through the secretory pathway in rice cell International conference

    「Kentarou Kaneko」「, Al Yanagida」「, Tsutomu Koshu」「Yuklho Umezawa」, 「Aya Kitaljma-Koga」「Maho Amano」「Shin-Ichrou Nishimura」「Toshiaki Mitsui」

    ICAR2010( 21st international conference on Arabidopsis Reserch)  2010.6 

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  • 翻訳後修飾因子ＯsSUMOタンパク質の細胞内局在化の研究

    齋藤智世, 五十嵐雄太, 永井理子, 近藤尚, 白矢武士, 北嶋(古賀)彩, 三ツ井敏明, 伊藤紀美子

    平成22年度 日本生化学会関東支部例会 第51回 新潟生化学懇話会 合同研究集会  2010.5 

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  • 翻訳後修飾因子OsSUMO タンパク質の細胞内局在化の研究

    齋藤智世, 五十嵐雄太, 永井理子, 近藤尚, 白矢武士, 北嶋(古賀)彩」, 三ツ井敏明, 伊藤紀美子

    平成22年度 日本生化学会関東支部例会 第51回 新潟生化学懇話会 合同研究集会  2010.5 

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  • プラスチド輸送が活発化しゴルジ体構造が変化したイネ細胞で発現上昇するタンパク質に関する研究

    中山勇希, 石山隆一, 高山和俊, 片峰拓紀, 朝倉剛, 三ツ井敏明

    平成22年度 日本生化学会関東支部例会 第51回 新潟生化学懇話会 合同研究集会  2010.5 

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  • 高温登熟性が異なる稲の発現特性解析

    森太紀, 白矢武士, 木村泉, 大久保英奈, 三ツ井敏明

    平成22年度 日本生化学会関東支部例会 第51回 新潟生化学懇話会 合同研究集会  2010.5 

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  • イネα-アミラーゼ I -1 プラスチド局在化シグナルの解析

    大久保英奈, 岡田久夫, 北嶋(古賀)彩, 濵田侑紀, 水谷理絵, 伊藤多絵子, 金古堅太郎, 白矢武士, 三ツ井敏明

    平成22年度 日本生化学会関東支部例会 第51回 新潟生化学懇話会 合同研究集会  2010.5 

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  • ヌクレオチドピロフォスファターゼ／ホスホジエステラーゼがイネのデンプン代謝に与える生理学的作用の解析

    甲州努, 金古堅太郎, 梅澤幸歩, 三ツ井敏明

    平成22年度 日本生化学会関東支部例会 第51回 新潟生化学懇話会 合同研究集会  2010.5 

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  • イネにおけるプラスチド局在糖タンパク質ヌクレオチドピロホスファターゼの糖鎖構造解析

    金古堅太郎, 甲州努, 梅澤幸歩, 北嶋(古賀)彩, 天野麻穂, 西村伸一郎, 豊岡公徳, 伊藤紀美子, 三ツ井敏明

    平成22年度 日本生化学会関東支部例会 第51回 新潟生化学懇話会 合同研究集会  2010.5 

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  • イネ葉緑体における糖タンパク質の解析

    濵田侑紀, 北嶋(古賀)彩, 水谷理絵, 大久保英奈, 伊藤多絵子, 金古堅太郎, 三ツ井敏明

    平成22年度 日本生化学会関東支部例会 第51回 新潟生化学懇話会 合同研究集会  2010.5 

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  • Mycobacterium tuberculosis H37 Rv3378c酵素の再検討

    大塚崇弘, 仲野千秋, 高山和俊, 三ツ井敏明, 佐藤努, 星野力

    日本農芸化学会2010年度(平成22年度)大会  2010.3 

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  • Plastid targeting of rice glycoprotein from the Golgi apparatus through the secretory pathway

    「Yuki Hamada」「Aya Kitajima-Koga」「Kentaro Kaneko」「Azwan Awang」「Toshiaki Mitsui

    Perspective of Plant Science 2010 (JSPS Invitational Training Program for Advanced Japanese Research Institutes  2010.3 

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  • イネNPP糖タンパク質におけるプラスチド局在化機構

    柳田愛, 金古堅太郎, 甲州努, 古賀(北嶋)彩, 伊藤紀美子, 三ツ井敏明

    日本農芸化学会2010年度(平成22年度)大会  2010.3 

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  • アミロライスの開発とその特性の研究(2)

    阿部克, 関川晶子, 伊地知和久, 花城勲, 峰村貴央, 佐藤かお理, 阿久澤さゆり, 藤田直子, 三ツ井敏明, 伊藤紀美子

    日本育種学会 第117回  2010.3 

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    Venue：京都大学  

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  • Proteome analysis of Theobroma cacao pod husk

    「Awang Azwan」「Rafiah Karim」「Toshiaki Mitsui」

    第51回日本植物生理学会年会  2010.3 

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  • イネにおけるプラスチド局在糖タンパク質ヌクレオチドピロホスファターゼ(NPP) の糖鎖構造解析

    金古堅太郎, 柳田愛, 甲州努, 梅澤幸歩, 古賀(北嶋)彩, 天野麻穂, 西村伸一郎, 伊藤紀美子, 三ツ井敏明

    第51回日本植物生理学会年会  2010.3 

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  • イネにおけるプラスチド局在糖タンパク質ヌクレオチドピロフォスファターゼの糖鎖構造解析

    金古堅太郎, 柳田愛, 甲州努, 天野麻穂, 西村紳一郎, 三ツ井敏明

    第32回日本分子生物学会年会  2009.10 

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  • Proteomics analysis of cocoa pod borer (Conopomorpha cramerella) resistant and susceptible cocoa (Theobroma cacao) varieties

    Azwan AWANG, Rafiah KARIM, 三ツ井敏明

    第32回日本分子生物学会年会  2009.10 

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  • Characterization of the plant rice kinesin O12 that has a calponin homology domain

    Nozomi UMEZU, Kazunori KONDO, Toshiaki MITSUI, Shinsaku MARUTA

    第82回日本生化学会大会  2009.10 

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  • Structural changes of Golgi apparatus in rice cells which Golgi-to-Plastid traffic is actively occurred.

    Kazutoshi TAKAYAMA, Yuki NAKAYAMA, Hiroki KATAMINE, Tsuyoshi ASAKURA, Toshiaki MITSUI

    第82回日本生化学会大会  2009.10 

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  • Identification of proteins from Theobroma cacao, a non-model plant, by de novo sequence analysis of N-terminal derivatized peptides

    Azwan AWANG, Rafiah KARIM, Toshiaki MITSUI

    第82回日本生化学会大会  2009.10 

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  • PROTEOME analysis of Theobroma cacao using de novo sequence analysis of derivatized peptides International conference

    Azwan AWANG, Rafiah KARIM, Toshiaki MITSUI

    The 3rd International Symposium "Sustainability in Food Production, Agriculture and the Environment in Asia"  2009.9 

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  • Rice grain damages caused under higher temperature stress during ripening: A cell biological aspect International conference

    Toshiaki MITSUI, Aya KITAJIMA, Azwan AWANG, Kentaro KANEKO, Ai YANAGIDA, Hisao OKADA, Kazutoshi TAKAYAMA, Yuki HAMADA, Taiki MORI, Rie MIZUTANI, Tsutomu KOSHU, Yuki NAKAYAMA, Ena OHKUBO, Shin KIMURA, Takeshi SHIARAYA

    The 3rd international symposium "Sustainability in food production,agriculture and environment in asia"  2009.9 

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  • イネにおけるプラスチド局在糖タンパク質ヌクレオチドピロホスファターゼの糖鎖構造解析

    金古堅太郎, 柳田愛, 甲州努, 天野麻穂, 西村紳一郎, 三ツ井敏明

    第３回GFRG研究会シンポジウム  2009.8 

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    Venue：北海道大学  

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  • α-アミラーゼ糖タンパク質のプラスチド輸送が活発化されたイネ細胞におけるゴルジ体の構造変化

    高山和俊, 中山勇希, 片峰拓紀, 朝倉剛, 谷口正之, 三ツ井敏明

    第27回日本植物細胞分子生物学会（藤沢）大会  2009.7 

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    Venue：日本大学生物資源科学部湘南 キャンパス  

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  • Secretory carrier membrane protein 2 localizes in trans-Golgi network-derived cluster of vesicles moving separately from the Golgi apparatus. International conference

    Kiminori TOYOOKA, Yumi GOTO, Satoru ASATSUMA, Masato KOIZUMI, Toshiaki MITSUI, Ken MATSUOKA

    Plant Biology 2009  2009.7 

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    Venue：Hawaii Convention Center  

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  • Plastid-targeting of rice alpha-amylase glycoprotein from the Golgi apparatus through the secretory pathway. International conference

    Toshiaki MITSUI

    Plant Biology 2009  2009.7 

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    Venue：Hawaii Convention Center  

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  • Plastid-targeting of rice alpha-amylase glycoprotein from the Golgi apparatus through the secretory pathway International conference

    Aya KITAJIMA, Satoru ASATSUMA, Hisao OKADA, Yuki HAMADA, Kentaro KANEKO, Kiminori TOYOOKA, Ken MATSUOKA, Akihiko NAKANO, Toshiaki MITSUI

    Plant Biology 2009  2009.7 

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    Venue：Hawaii Convention Center  

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  • PROTEOMICS analysis of cocoa pod borer (Conopomorpha cramerella Snellen) resistant and susceptible pods International conference

    Azwan AWANG, Rafiah KARIM, Toshiaki MITSUI

    Malaysian International Cocoa Conference 2009  2009.4 

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  • 高温登熟性の異なるイネ品種の遺伝子発現特性解析（Ⅰ）

    森 太紀, 高山和俊, 片峰拓紀, 北嶋彩, 金古堅太郎, 三ツ井敏明

    日本農芸化学会大会2009年度大会  2009.3 

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    Venue：福岡国際会議場・マリンメッセ福岡  

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  • イネαアミラーゼⅠ-1プラスチド局在化シグナルの解析

    岡田久夫, 北嶋彩, 濱田侑紀, 金古堅太郎, 三ツ井敏明

    日本農芸化学会大会2009年度大会  2009.3 

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    Venue：福岡国際会議場・マリンメッセ福岡  

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  • 高アミロース米の研究

    阿部 克, 関川 晶子, 小澤 由美, 藤田 直子, 三ツ井 敏明, 大坪 研一, 伊藤 紀美子, 岸根 雅宏

    日本農芸化学会大会2009年度大会  2009.3 

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    Venue：福岡国際会議場・マリンメッセ福岡  

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  • イネADP-グルコース分解型NPPの生理機能の解析

    金古堅太郎, 山田智恵, 柳田愛, 北嶋彩, 伊藤紀美子, 三ツ井敏明

    日本植物生理学会第50回年会  2009.3 

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    Venue：名古屋大学東山キャンパス  

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  • アミロライスの開発とその特性の研究 (1)

    阿部 克, 関川 晶子, 小澤 由美, 長谷川 真理

    日本育種学会114回講演会  2008.9 

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  • イネα-アミラーゼI-1プラスチド局在化シグナルの解析：部位特異的変異によるプラスチド局在化と酵素活性への影響

    岡田久夫, 北嶋彩, 金古堅太郎, 濱田侑紀, 伊藤紀美子, 三ツ井敏明

    第31回日本分子生物学会年会、第81回日本生化学会大会  2008.9 

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  • GAMYB遺伝子の転写後、翻訳後調節の解析

    五十嵐雄太, 宮嵜敬弘, 近藤尚, 永井理子, 南條洋平, 三ツ井敏明, 伊藤紀美子

    第31回日本分子生物学会年会、第81回日本生化学会大会  2008.9 

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  • STUDY FOR CELLUER LOCALIZATION OF RICE SUMO1 AND SUNO2 International conference

    Yuta IKARASHI, Nao kONDO, Aya KITAJIMA, Toshiaki MITSUI, Kimiko ITOH

    ZOMESⅤThe Fifth international Symposium on COP9 signalosome,Proteasome and elF3:At the interface between signaling&proteolysis  2008.9 

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  • Comparative proteomics studies between cocoa pod borer (Conopomorpha cramerella Snellen) resistant and susceptible cocoa (Theobroma cacao) clones

    Awang AZWAN, Karin RFIASH, Kazutoshi TAKAYAMA, Douglas FURTEK, Toshiaki MITSUI

    第31回日本分子生物学会年会、第81回日本生化学会大会  2008.9 

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  • イネヌクレオチドピロホスファターゼ/ホスホジエステラーゼ1におけるクロロプラスト局在化に関与する配列の解析

    山田智恵, 金古堅太郎, 柳田愛, 北嶋彩, 伊藤紀美子, 三ツ井敏明

    第31回日本分子生物学会年会、第81回日本生化学会大会  2008.9 

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  • イネヌクレオチドピロホスファターゼ/ホスホジエステラーゼ1の機能解析

    柳田愛, 金古堅太郎, 山田智恵, 北嶋彩, 伊藤紀美子, 三ツ井敏明

    第31回日本分子生物学会年会、第81回日本生化学会大会  2008.9 

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  • イネ糖タンパク質α‐アミラーゼI-1のゴルジ体-プラスチド間輸送に関する研究

    北嶋彩, 岡田久夫, 浜田侑紀, 豊岡公徳, 三ツ井敏明

    第31回日本分子生物学会年会、第81回日本生化学会大会  2008.9 

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  • 高等植物細胞におけるゴルジ‐プラスチド間輸送

    北嶋彩, 唐橋あゆみ, 高田頌, 豊岡公徳, 浅妻 悟, 松岡健, 中野明彦, 三ツ井敏明

    大阪大学蛋白質研究所セミナー  2008.9 

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  • Secretory Vesicle Cluster: トランスゴルジネットワーク由来の分泌系後期で働く新奇構造体

    松岡健, 豊岡公徳, 浅妻悟, 後藤友美, 三ツ井 敏明

    日本植物生理学会第49回年会  2008.9 

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  • イネNPP遺伝子ファミリーの機能解析-NPP1,2及び6は葉緑体に局在する

    金古堅太郎, 山田智恵, 柳田愛, 北嶋彩, 伊藤紀美子, 三ツ井敏明

    日本植物生理学会第49回年会  2008.9 

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  • イネ唐タンパク質α‐アミラーゼI-1のプラスチドターゲティング：ゴルジ‐プラスチド間輸送の解析

    北嶋彩, 唐橋あゆみ, 高田頌, 豊岡公徳, 浅妻 悟, 松岡健, 中野明彦, 三ツ井敏明

    日本植物生理学会第49回年会  2008.9 

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  • イネNPP遺伝子ファミリーの機能解析～糖代謝に及ぼす影響～

    金古堅太郎, 山田智恵, 柳田愛, 伊藤紀美子, 三ツ井敏明

    日本農芸化学会大会2008年度大会  2008.9 

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  • イネにおけるデンプン代謝関連酵素のプラスチドターゲティング～ 米品質低下とのかかわり～

    北嶋 彩, 金古 堅太郎, 山田 智恵, 岡田 久夫, 柳 田愛, 高山 和俊, 森 太紀, 濱田 侑紀, 三ツ井 敏明

    東京理科大学総合研究機構再生工学研究センターシンポジウム  2008.9 

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  • イネヌクレオチドピロホスファターゼ/ホスホジエステラーゼの機能解析

    柳田 愛, 金子 堅太郎, 山田 智恵, 伊藤 紀美子, 三ツ井 敏明

    日本応用糖質科学会平成20年度大会  2008.9 

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  • Rice Golgi Proteome: Structural Changes of Golgi Apparatus in Rice Cells overexpressing α-AmylaseI-1 International conference

    Hiroki KATAMINE, Tsuyoshi ASAKURA, Makoto AOYAMA, Kazutoshi TAKAYAMA, Hitoshi MORI, Toshiaki MITSUI

    International Symposium on Frontier in Plant Protiome Research  2008.3 

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  • Plastid Targeting of Glycoproteins Involved in Starch Metabolism in Higher Plant Calls International conference

    Toshiaki MITSUI, Aya KITAJIMA, Ayumi KARAHASHI, Kentaro KANEKO, Chie YAMADA, Hiroki KATAMINE

    International Symposium on Frontier in Plant Protiome Research  2008.3 

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  • Development of insect-resistant cocoa trees through proteomics International conference

    Awang AZWAN, Douglas FURTEK, Toshiaki MITSUI

    International Symposium on Frontier in Plant Protiome Research  2008.3 

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  • イネゴルジ複合体プロテオーム：糖タンパク質過剰発現細胞におけるN-AcetylglucosaminyltransferaseI結合ゴルジ膜の解析

    朝倉 剛, 片峰拓紀, 青山 慎, 廣瀬将太, 行方由美, 三ツ井 敏明

    日本植物生理学会第48回年会  2007.9 

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  • 無細胞翻訳系で合成したイネα‐アミラーゼI-1の酵素学的諸性質

    唐 橋 あゆみ, 北 嶋 彩, 高 田, 頌, 澤 田 千穂子, 浅 妻, 悟, 三ツ井 敏

    日本農芸化学会大会2007年度大会  2007.9 

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  • 胚乳特異的RNAi法による高アミロース成分米の研究

    関川晶子, 小澤由美, 長谷川 真理, 倉冨裕樹, 花城 勲, 竹田靖史, 三ツ井 敏明, 伊藤 紀美子

    日本農芸化学会大会2007年度大会  2007.9 

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  • Crystal structure of ADP bound motor domain of the rice kinesin K16 reveals a novel conformation. International conference

    Shinsaku MARUTA, Nobuhisa UMEKI, Nozomi UMEZU, Yuko KUBO, Zui FUJIMOTO, Toshiaki MITSUI

    Biophysical Society 51st Annual Meeting  2007.3 

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  • イネゴルジ複合体プロテオーム：GFP-SYP31 標識シスゴルジ膜画分の解析

    朝倉 剛, 廣瀬将太, 片峰拓紀, 佐藤雅彦, 藤原正幸, 島本 功, 堀 秀隆, 三ツ井 敏明

    日本植物生理学会第47回年会  2006.9 

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  • Targeting of rice glycoprotein α-amylaseI-1 into plastid : targeting pathway to plastid International conference

    Aya KITAJIMA, Satoru ASATSUMA, Chihoko SAWADA, Ayumi KARAHASHI, Akihiko NAKANO, Toshiaki MITSUI

    Dynamic Organelles in Plants  2006.9 

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  • Characterization of a plastidial N-glycosylated nucleotide pyrophosphatase/phosphodiesterase in rice. International conference

    Hiromasa OKA, Noriko IKARASHI, Kentaro KANEKO, Yohei NANJO, Javier POZUETA-ROMERO AND, Toshiaki MITSUI

    Dynamic Organelles in Plants  2006.9 

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  • Characterizations of a rice plant specific kinesin O12 International conference

    Nozomi UMEZU, Nobuhisa UMEKI, Kazunori KONDO, Toshiaki MITSUI, Shinsaku MARUTA

    20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress  2006.9 

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Industrial property rights

  • 新大コシヒカリ

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    Application no：商願 2023-026470  Date applied：2023.3

    Patent/Registration no：商標登録第6721358号  Date registered：2023.7 

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  • イネの環境ストレス耐性を付与する方法

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    Application no：特願2019-226340  Date applied：2019.12

    Date announced：2021.6

    Publication no：2021-093933 

    Patent/Registration no：特許第7319671号  Date registered：2023.7 

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Awards

  • 第8回食の新潟国際賞地域未来賞

    2024.11   公益社団法人 食の新潟国際賞  

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  • 第77回新潟日報文化賞 産業技術部門

    2024.10  

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  • 刈羽村村政功労者

    2024.1   刈羽村   村政ならびに産業の発展に貢献

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  • 優秀発表賞

    2021.10   日本作物学会第252回講演会   休眠遺伝子Sdr4-k の導入による酒米の品質向 上

    金澤伸矢、バスラム マロワン、三ツ井敏明他

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  • ポスター賞

    2021.9   日本応用糖質科学会   休眠遺伝⼦Sdr4-k導⼊が酒⽶に与える影響の解析

    金澤伸矢, バスラム マロワン, 三ツ井敏明他

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  • 日本応用糖質科学会 学会賞

    2018.6   日本応用糖質科学会   イネにおける澱粉代謝関連酵素の応用分子細胞生物学的研究

    三ツ井敏明

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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  • 2014年BBB論文賞

    2015.3   公益社団法人 日本農芸化学会   Crystal structure of α-amylase from Oryza sativa: Molecular insights into enzyme activity and thermostability.

    Ochiai, A, Sugai, H, Harada K, Tanaka, S, Ishiyama, Y, Ito, K, Tanaka, T, Uchiumi, T, Taniguchi, M, Mitsui, T

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    Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

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  • 日本作物学会 第238回講演会優秀発表賞（口頭発表部門）

    2014.10   日本作物学会   稲の高温登熟障害発生を診断できる遺伝子発現マーカーの開発

    白矢武士, 三ツ井敏明

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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  • 第55回新潟生化学懇話会ポスター発表優秀賞

    2014.6   新潟生化学懇話会   イネ葉緑体包膜タンパク質LACS9の輸送機構の研究

    高松壮, 伊東七実子, 谷内智子, 古賀彩, 濵田侑紀, 三ツ井敏明

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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  • 2012年 農林水産研究成果10大トピックス 第１位

    2012.12   農林水産技術会議事務局  

    三ツ井敏明

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    Award type：International academic award (Japan or overseas)  Country：Japan

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  • 日本応用糖質学会平成24年度大会（第61回） ポスター賞

    2012.9   日本応用糖質学会  

    丸山達也, 佐々木麻衣子, 白矢武士, 金古堅太郎, 三ツ井敏明

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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  • 鈴木紘一メモリアル賞（優秀プレゼンテーション賞）

    2011.9   第84回日本生化学会大会  

    甲州 努, 金古 堅太郎, 梅澤 幸歩, 三ツ井 敏明

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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  • 鈴木紘一メモリアル賞（優秀プレゼンテーション賞)

    2011.9   第84回日本生化学会大会  

    白矢 武士, 森 太紀, 大久保 英奈, 丸山 達也, 金古 堅太郎, 三ツ井 敏明

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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  • 農芸化学奨励賞

    1999.1   社団法人 日本農芸化学会  

    三ツ井敏明

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    Award type：International academic award (Japan or overseas)  Country：Japan

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Research Projects

  • マルチストレス耐性イネ品種の機能発現メカニズム

    Grant number：24K08825

    2024.4 - 2027.3

    System name：科学研究費助成事業

    Research category：基盤研究(C)

    Awarding organization：日本学術振興会

    三ツ井 敏明

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

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  • 母体の細胞質ゲノム関与による新しい耐塩性イネの育種の確立

    Grant number：23KF0033

    2023.4 - 2025.3

    System name：科学研究費助成事業 特別研究員奨励費

    Research category：特別研究員奨励費

    Awarding organization：日本学術振興会

    三ツ井 敏明

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    Grant amount：\2000000 （ Direct Cost: \2000000 ）

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  • 気候変動下における穀物資源の利用効率化に関する包括研究

    Grant number：16809888

    2017 - 2021

    Research category：国際的な科学技術共同研究などの推進/国際科学技術共同研究推進事業/SICORP/CONCERT-Japan(SICORP)

    Awarding organization：科学技術振興機構

    三ツ井 敏明

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    本研究は、さまざまな環境下における穀物の生産性および品質並びに養分利用効率を制御する作物学的、生理学的、分子的ボトルネックを特定する。また、気候パラメータの変化を考慮した養分利用効率を最適化するための予測モデルの開発を行う。 具体的には、日本側はイネ研究を実施し、スペイン側は小麦・新穀物研究、フランス側はメタボローム研究、そしてドイツ側はモデル構築を実施する。 日欧の研究チームが相互補完的に取り組むことにより、世界人口の増加と変化するグローバル環境に対応する農業作物の高生産性および栄養価の最適化技術の開発が期待される。

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  • High temperature resistance of the grain-filling depending on low grain growth rate and high assimilation in rice

    Grant number：15H04444

    2015.4 - 2019.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    Kobata Tohru

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    Grant amount：\16380000 （ Direct Cost: \12600000 、 Indirect Cost：\3780000 ）

    High temperature resistance in rice grain yield and quality is expected. Cultivars of a less increase in the grain filling under high temperature conditions was selected from rice of the world and Japan rice core collection. The less increase in the grain filling rate was expected to result a high temperature resistance of the grain filling because of relief in a lack of assimilate supply to the grain and keeping starch accumulation in grains. When panicles from these collection cultivars were solution cultured under 30 C, grain filling rate indicated diverse variability. The variability for selected typical cultivars was evidenced under diverse temperature and pot cultivated conditions. Metabolic processes for starch synthesis would relate with the cultivar difference in a response of the grain filling to high temperatures.

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  • Development studies on high temperature and high CO2 tolerant rice during seed ripening stage

    Grant number：15H02486

    2015.4 - 2019.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (A)

    Awarding organization：Japan Society for the Promotion of Science

    MITSUI Toshiaki

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    Grant amount：\41600000 （ Direct Cost: \32000000 、 Indirect Cost：\9600000 ）

    In this study, we performed proteome and starch glycome analyses of high temperature ripened brown rice, and concluded that the chalking of grain was caused by the imbalance between starch synthesis and degradation. In addition, when the effects of strong light ＆ high CO2 concentration and high temperature＆ high CO2 concentration on appearance quality of rice grains were examined, the sensitivity was shown to be high in the early stage of the ripening period from flowering. However, it was found that high CO2 promotes the effects of high temperature stress, although remarkable chalkiness of brown rice does not occur only under high CO2 conditions. Mn-type superoxide dismutase (MSD1) was identified as a key enzyme involved in high temperature tolerance of rice, and it was demonstrated that high temperature ripening tolerance is improved by the constitutively high expression of MSD1 gene, while the suppression of MSD1 gene markedly enhanced the high temperature susceptibility.

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  • Development of palatable and indigestible processed rice and elucidation of mechanism by biological and physical method

    Grant number：15H02891

    2015.4 - 2018.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    Ohtsubo Ken'ichi

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    Grant amount：\17420000 （ Direct Cost: \13400000 、 Indirect Cost：\4020000 ）

    The objective of this research is to develop a palatable and bio-functional (low-digestible) rice product and to elucidate the mechanism. Using ordinary rice, giant-embryo rice or super-hard rice, we evaluated eating quality and low-digestibility, and it became possible to improve the characteristics. In case of the super-hard rice, it was clarified that the starch properties, especially contents of medium to long chains of the amylopectin, affect the eating quality and low-digestability through the physical properties and pasting properties. In the case of giant-embryo rice, it was indicated that germination was accelerated by the addition of red onion through the increase of phyto-hormone like IAA and high-pressure treatment improves palatability through the activation of enzymes and change in starch structure.

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  • イネ澱粉集積抑制酵素変異体における高二酸化炭素・高温応答メカニズムの解明

    Grant number：24114705

    2012.4 - 2014.3

    System name：科学研究費助成事業

    Research category：新学術領域研究(研究領域提案型)

    Awarding organization：日本学術振興会

    三ツ井 敏明

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    Grant amount：\25220000 （ Direct Cost: \19400000 、 Indirect Cost：\5820000 ）

    （１）イネ糖タンパク質のプラスチド局在化機構：イネプラスチド糖タンパク質であるヌクレオチドピロホスファターゼ／ホスホジエステラーゼ１（NPP1）におけるN-結合型糖鎖の結合部位をアーモンドグリコペプチダーゼA／nLC-MS/MSを用いて解析し、326NTTと579NHSが糖鎖結合部位であることが分かった。イネおよびアラビドプシス緑葉から葉緑体をパーコール密度勾配遠心法により単離し、葉緑体N-グライコームをグライコブロッティング／MALDI-TOF-MS法を用いて解析したところ、イネ葉緑体N-グライコームはアラビドプシスのそれに比べ量的・質的に複雑であることが示唆された。
    （２）イネ澱粉集積抑制酵素変異体の体温および光合成能に及ぼす高CO2・高温環境の影響: NPP1遺伝子の生理機能を明らかにするために、NPP1遺伝子のTos17挿入変異体npp1の形質を調べた。npp1変異体の幼芽のADP-グルコース分解活性が野生型の約20％となっていたことから、NPP1がイネおける主要なADP-グルコース分解酵素であることがわかった。npp1変異体では、温度条件、CO2濃度条件にかかわらず、野生型（WT）に比べて成長が早く、澱粉蓄積も高まることがわかった。また、サーモグラフィー観察からnpp1変異体の体温がWTと比較して低いことが見出された。さらに、種々のCO2濃度・温度環境下におけるnpp1変異体のCO2吸収・光合成能を解析した結果、すべての条件においてnpp1変異体のCO2吸収・光合成能が向上していた。特に、高CO2・高温環境下（33℃/28℃；1,600 ppm CO2）においてnpp1変異体のCO2吸収能がWTに比べて高かった。以上の結果から、NPP1遺伝子は気孔開閉、光合成並びに澱粉集積を負に制御する機能を有するものと結論された。

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  • イネを用いた高効率CO2吸収・資源化システムの開発

    Grant number：11102896

    2011 - 2012

    Research category：戦略的な研究開発の推進/戦略的創造研究推進事業/ALCA(先端的低炭素化技術開発)/探索ステージ

    Awarding organization：科学技術振興機構

    三ツ井 敏明

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  • Analysis of plastid-targeting mechanism of glycoproteins involved in starch metabolism of rice

    Grant number：22380186

    2010.4 - 2014.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    MITSUI Toshiaki, ITOH Kimiko, HAYASHI Yasuko, HANASHIRO Isao, OHTSUBO Ken-ichi

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    Grant amount：\18200000 （ Direct Cost: \14000000 、 Indirect Cost：\4200000 ）

    (1) We found that rice AmyI-1, NPP1, NPP2, NPP6, and MSD1 are glycoproteins targeting to plastid via the secretory pathway, and we characterized N-glycome (oligosaccharide structures) of plastidial glycoprotein NPP1. (2) The plastid targeting signal regions (PT) of AmyI-1 and NPP1 were identified. Furthermore, it was suggested that there exists plastid envelope factor(s) that required for plastid targeting of glycoprotein. (3) Abnormal expression of alpha-amylase during seed development led to the poor formation of starch granule. In addition, NPP1 was found to be a negative regulator for starch accumulation in rice leaves.

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  • イネデンプン集積抑制酵素発現制御体の高CO_2応答に関する研究

    Grant number：22114507

    2010 - 2011

    System name：科学研究費助成事業

    Research category：新学術領域研究(研究領域提案型)

    Awarding organization：日本学術振興会

    三ツ井 敏明

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    Grant amount：\21710000 （ Direct Cost: \16700000 、 Indirect Cost：\5010000 ）

    平成23年度の研究成果は下記の通りである。
    (1)イネ澱粉代謝関連糖タンパク質のプラスチド局在化機構の解明:イネヌクレオチドピロホスファターゼ/ホスホジエステラーゼ糖タンパク質アイソザイム(NPP1、2、3、6)の遺伝子にGFPを連結し、イネ細胞に導入、発現させ、それらの葉緑体局在化能をレーザー共焦点蛍光顕微鏡で観察した。NPP2-GFPとNPP6-GFPはNPP1-GFPと同様に葉緑体に局在したが、NPP3-GFPは葉緑体局在化能を持たないことが明らかになった。NPP1、2および6のN-グライコーム解析から、NPP糖タンパク質のプラスチドターゲティングにトランスゴルジが深く関与することが推察された。さらに、トランスゴルジマーカー遺伝子ST-mRFPをイネに導入し、その挙動を観察したところ、NPP1強発現下でST-mRFP標識膜小胞が活発に葉緑体に取り込まれることが見いだされた。これらの結果から、NPP糖タンパク質のプラスチド局在化機構にトランスゴルジ体-プラスチド間の膜交通が重要な役割を果たしていることが強く示唆された。
    (2)イネ澱粉集積抑制酵素機能欠損変異体の澱粉集積特性に及ぼす高CO_2濃度および高温の影響:植物CO_2インキュベータを用いてTos17挿入変異体npp1(日本晴)の初期成長期(10～17d)の生重量と澱粉蓄積量に及ぼす高CO_2濃度(1,200ppm)、高温(昼12h,33℃、夜12h,28℃)の影響を調べた。npp1変異体シュートにおいては、対照条件(光:300μmol/m^2/sec、CO_2濃度:400ppm、温度:昼12h,28℃、夜12h,23℃)から高CO2条件に移すと顕著な生重量と澱粉蓄積量の増加が観察された。澱粉蓄積については高CO_2・高温条件に置くと増加傾向がより高まることが見いだされた。このnpp1変異体の高CO_2応答機構を探るために、iTRAQ標識法を用いた定量的ショットガンプロテオミクス解析を試みた。高CO_2条件下においてnpp1変異体のタンパク質発現は野生型に比べて大きく変動することが観察され、npp1変異体が高CO_2に敏感に応答しているものと考えられた。

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  • 環境適応に関わるゴルジ複合体の構造・機能解析

    Grant number：17051011

    2005 - 2006

    System name：科学研究費助成事業

    Research category：特定領域研究

    Awarding organization：日本学術振興会

    三ツ井 敏明

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    Grant amount：\6000000 （ Direct Cost: \6000000 ）

    本課題研究においては、環境に応答して変化するゴルジ体の形態と構成膜タンパク質を網羅的に解析し、環境適応に関わる植物ゴルジ体特有な構造・機能を解明することを目的として、(1)イネゴルジ複合体構成タンパク質の網羅的解析、(2)デンプン代謝関連酵素であり且つ糖タンパク質であるα-アミラーゼを強発現する形質転換イネにおけるゴルジ体の構造変化について解析を行った。
    (1)ショ糖密度勾配遠心・沈降法およびフローティング法を駆使して、イネゴルジ体膜を単離し、イネゴルジ体膜に存在するタンパク質の同定を試みたところ、糖鎖合成・構築関連酵素タンパク、イオンやpHホメオスタシスにかかわるタンパク、小胞輸送にかかわるタンパク、ゴルジマトリックスタンパク、機能未知なendomembrane protein EMP70などが検出された。(2)α-アミラーゼ(AmyI-1)あるいはシスゴルジマーカー(GFP-SYP31)を強発現する形質転換イネ細胞を作成した。それぞれの形質転換細胞からN-acetylglucosaminyltransferase-I(GNT-I)を指標にゴルジ体膜を分画した。抗イネGNT-I抗体を用いたイムノブロット解析の結果、AmyI-1強発現細胞から調製したGNT-I結合ゴルジ体膜の膜密度が野生型細胞のそれと比較して高くなることが見いだされた。一方、GFP-SYP31発現細胞ではこのような現象は見られなかった。さらに、AmyI-1強発現細胞の電子顕微鏡観察からこの細胞においてはゴルジ装置の数の変化も見られることが分かった。これらの結果は、AmyI-1強発現イネ細胞においてはゴルジ体が野生型細胞とは異なる機能タンパク質や脂質で構成されている可能性を強く示唆した。

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  • Studies for structural change of amylopectin by granule-bound starch synthase I and pasting property of the starch granules.

    Grant number：16380084

    2004 - 2006

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    ITOH Kimiko, HANASHIRO Isao, MITSUI Toshiaki

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    Grant amount：\15800000 （ Direct Cost: \15800000 ）

    A rice Wx^a gene encoding for a granule-bound starch synthase I (GBSSI) was introduced into japonica-type waxy rice (cultivars Iwaimochi and Musashimochi) and its effect on structures and pasting properties of endosperm starches was examined. Apparent amylose content determined by high-performance size-exclusion chromatography of debranched starch increased from undetectable amount for the parent waxy cultivars to 21.6-22.2% for the transgenic lines. Similar analyses of debranched amylopectin revealed existence of a significant amount (7.5-8.4% by weight) of long unit-chains (LC), which appeared in the same elution position as amylose. Taking into account the LC content, actual amylose content was calculated to be 14.9-16.0% for the transgenic lines. No significant difference was found in chain-length distribution for the A, B_1, and B_2+B_3 fractions, indicating the effect by the transgene on amylopectin structure was essentially specific in LC formation. Since Labelle amylopectin contains LC only slightly (0.6% by weight), as yet determined factor(s) other than GBSS activity is thought to be involved in control of formation and/or the amount of LC formed. LC isolated from debranched amylopectin by 1-butanol precipitation exhibited number-average degree of polymerization (DPn) of 350-390 and DP distribution distinct from amylose. Structure of amylose was slightly different between Labelle and the transgenic lines, and yet the latter amyloses had characteristics of indica rice rather than japonica rice. Pasting properties examined with a Rapid Visco Analyzer showed the GBSS expression caused elevation of pasting temperature, a decrease in peak viscosity by half, a large decrease in breakdown, indicating the altered starch structures resulted in starch granules less susceptible to swelling.
    Wx genomic DNA was analyzed and structural differences were determined between non-LC type cultivar and LC-type cultivar or transgenic lines. The two point mutations were commonly found in genomic structure at non-LC type cultivar and the mutations can lead to changes in amino acids sequence of WX protein.

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  • 葉緑体における新規な局在化メカニズム

    Grant number：16658042

    2004 - 2006

    System name：科学研究費助成事業

    Research category：萌芽研究

    Awarding organization：日本学術振興会

    三ツ井 敏明

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    Grant amount：\3500000 （ Direct Cost: \3500000 ）

    葉緑体は、光合成機能を有する重要な細胞内小器官である。また、葉緑体が分化したアミロプラストは、植物のみならず地球上のすべての生命にとって最も重要なエネルギー蓄積過程の1つである光合成産物のデンプンへの集積を行う。本研究課題は、分子細胞生物学的研究手法を駆使して穀類細胞において核ゲノムにコードされている酵素タンパク質の葉緑体・アミロプラストへのターゲティング機構を再検討し、プラスチド構成タンパク質の新規なターゲティング機構の存在を明らかにすることを目的とした。これまでの研究から、プラスチド局在化に関わる典型的なトランジットペプチドを有しないヌクレオチドピロホスファターゼ/ホスホジエステラーゼ(NPP1)およびα-アミラーゼI-1(AmyI-1)は、ともに分泌経路(小胞体-ゴルジ体系)からプラスチドへ輸送・局在化することが示唆された。そこで、NPPIおよびAmyI-1のプラスチド局在化シグナルを決定するために、これらのポリペプチド鎖の種々の領域をコードする遺伝子と緑色蛍光タンパク質(GFP)遺伝子との融合遺伝子をアグロバクテリウム法あるいはパーテクルガン法によりイネ細胞あるいはタマネギ細胞に導入し、発現したキメラタンパク質のプラスチドへのターゲティングを蛍光顕微鏡、共焦点レーザー顕微鏡を用いて観察した。その結果、NPP1およびAmyI-1前駆体のN-末端にある小胞体シグナル配列のみではプラスチド局在化はできないことが分かった。さらに、AmyI-1において絞り込んだシグナル候補領域に点突然変異を導入し、プラスチド局在化能を解析した結果、プラスチドターゲティングシグナルは特定のアミノ酸配列ではなく、その領域の立体構造が重要であることが示唆された。

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  • Recognition mechanism between insect midgut epithelial cell membrane and insecticidal cry toxins

    Grant number：13306006

    2001 - 2003

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (A)

    Awarding organization：Japan Society for the Promotion of Science

    HORI Hidetaka, MITUI Toshiaki, SATO Ryoichi, HAYAKAWA Tohru

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    Grant amount：\35360000 （ Direct Cost: \27200000 、 Indirect Cost：\8160000 ）

    Interaction between insecticidal Cry toxins and apical cell membrane from the silkworm, Bombyx mori, midgut was investigated.
    Four main results were obtained. 1)Novel P252 which bound Cry1Aa, Cry1Ab and Cry1Ac was discovered from brush border membrane. 2)Novel aminopeptidase, AP, which bound only Cry1Ac was discovered. This novel AP was thought to belong to group 1. 3)Liposomes containing BBM proteins were constructed and shown to have twice higher affinity with Cry1Aa, Cry1Ab and Cry1Ac compared to the liposome without the proteins. 4)Cry1A toxins have been thought to insert their α-helices in domain 1. But nothing is clear about which helices inserted into the membrane. We determined the portion of domain 1 which was inserted into apical cell membrane. About 24 kDa peptides has been thought to be inserted and it was shown to correspond to the stretch from α-helix 2 to α-helix 7. This was first concrete suggestion about the insertion region of domain 1.
    P252 was solubilized with Triton X-100 or CHAPS and thus it was suggested to be localized in non raft region. P252 was thought to be a glycoprotein with homo tetramer. The sugar side chain was suggested to be tri antennal N-linked chain containing bisecting GlcNAc and O-linked sugar side chain with GalNAc. Value of kd of P252 with Cry1Aa, Cry1Ab and Cry1Ac were 10nM, 70nM and 20nM, respectively and which were thought to be one of the strongest bin ding in references. Heterologous binding assay suggested that the site of P252 for Cry1Aa shared by 50% with Cry1Ac and it for Cry1Ac shared by 80% with Cry1Aa. The value of kd of P252 with Cry1Ab was 70 nM and the value still low enough to thought that the binding was physiological event. But interestingly, the site for Cry1Ab was shared with Cry1Aa and Cry1Ab by 50% each and BSA also was shown to inhibit the binding.
    P96 was recognized with antibody of APN 1. P96 was shown to bind only Cry1Ac among three Cry1A toxins and the binding was inhibited with GalNAc. Substrate specificity was surveyed and P96 was suggested to be APN. The reaction required divalent metal cations and optimum pH was 6-8. Reaction speed was enhanced at higher substrate concentration.

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  • Analysis of starch-to-sucrose transition mechanism in germinating rice seed and its application

    Grant number：12660071

    2000 - 2001

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    MITSUI Toshiaki

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    Grant amount：\3500000 （ Direct Cost: \3500000 ）

    Aim of this research project is to clarify the starch-to-sucrose transition mechanism in germinating rice seed and to establish control system of seed germination. The results obtained in this research period are as follow:
    1. Temporal, special and hormone-regulated expression of α-amylase and UDP-glucose pyrophosphorylase (UGPase) in germinating rice seeds: UGPase was concluded to be house-keeping enzyme in the scutellar and aleurone tissues. Temporal and spatial expressions of α-amylase I-1 and II-4 were essentially identical in the aleurone layer, although these were distinguishable in the embryo tissues at the early stage of germinatioa It was found that the post-transcriptional regulation of expression of α-amylase II-4, that lacks the gibberellin (GA) response cis-element in the promoter region of the gene, by GA operates in the aleurone layer of germinating rice seed, which is mediated by Ca^<2+> and calmodulin.
    2. Involvement of histone acetylation in transcription of α-amylase genes: A potent inhibitor for histone deacetylase, Trichostatin A increased the level of α-amylase mRNA in the aleuome without GA, suggesting that the histone acetylation is involved in the regulation of α-amylase gene expression.
    3. Proteome analysis of scuteller tissues: In the scutellar tissues of germinating rice seeds, several proteins that exhibited the time-dependent and abscisic acid (ABA)-inducible expression were listed up. Glycolitic enzymes, such as phosphoglycerate mutase, glyceraldehydes-3P dehydrogenase, etc., were identified in these proteins. GS-129, one of the listed proteins exhibited the following characteristics: (a) GS-129 tightly associated with cell wall materials, (b) GS-129 was glycoprotein bearing both N-linked and O-linked saccharide chains, (c) GS-129 was suggested to be a possible xylan hydrolyzing enzyme.

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  • Molecular mechanism of regulation of rice seed germination

    Grant number：10640628

    1998 - 1999

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    MITSUI Toshiaki

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    Grant amount：\3500000 （ Direct Cost: \3500000 ）

    Aim of this research project is to clarify the molecular mechanism of germination of rice seeds. The results obtained in this research periods are as follow:
    1. The expression of α-amylase in the scutellar tissues and the aleurone layer of germinating rice seeds was differentially regulated by gibberellin (GA), abscisic acid (ABA), and metabolic sugars. The α-amylase expression in aleurone layer was induced by GA and the GA effect was suppressed by ABA, while the α-amylase expression in scutellum was strongly reduced by glucose and ABA but not ABA alone.
    2. Sucrose controlled the intracellular transport of α-amylase and stimulated its turnover in rice cells. Sugar also controlled the CaィイD12+ィエD1 uptake into rice cells, and there were a close relationship between the α-amylase expression and the CaィイD12+ィエD1 uptake.
    3. ABA stimulated the glucose uptake and sucrose synthesis in the scutellar tissues. It was also found that sucrose synthesis occurred in the aleurone layer, and that the sucrose synthesis was inhibited by GA and the GA effect was suppressed by ABA.
    4. The inositol 1,4,5-trisphosphate (IPィイD23ィエD2)-CaィイD12+ィエD1 signal transduction was suggested to be involved in the seed germination and the GA-inducible α-amylase expression. The level of IPィイD23ィエD2 in the aleurone layer was increased by GA. GA also induced the inositol phospholipid-specific phospholipase C in the aleurone.
    Overall results indicated that the close talk of GA, ABA and metabolic sugars is involved in the regulation of α-amylase expression and germination of rice seed.

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  • Studies on structure and generation of volatile formating gene in edible mushrooms

    Grant number：07456154

    1995 - 1997

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    HAYAKAWA Toshiro, JOH Toshio, MITSUI Toshiaki

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    Grant amount：\1100000 （ Direct Cost: \1100000 ）

    Induction of lipoxygenase in mycelia of Shiitake mushroom (Lentinus edodes) was studied.
    Fresh weight of mycelia cultured for 3 weaks in soybean medium, in which soybean oil and flour were respectively used as carbon and nitrogen sources, was 11-fold that in GC medium, in which goucose and casamino acids were respectively used as carbon and nitrogen sources. Mycelial forms of both cultures differed greatly : GCculture, a large spherical form ; soybean soybean culture, a small spherical form or a fibriform. Lipoxygenase activity of mycelia cultured in soybean medium was 26-fold higher than the corresponding activity in GC medium, indicating the induction of lipoxygenase in mycelia by soybean culture. Optimum concentrations of soybean oil and flour in the medium for the growth of mycelia and the induction of lipoxygenase were 60 ml/1 and 12g/1, respectively. Active staining of lipoxygenase revealed that among 4 lipoxygenase isozymes in mycelia of GC culture only one isozyme is induced by soybean culture. The induced lipoxygenase showed high activity below pH 4.5, and was stable at pH 3.0 to 8.5 and below 50ﾟC.The enzyme had a higher specificity against linoleic acid and arachidonic acid than against linolenic acid. Cu^<2+>, N-ethylmaleimide and indoactic acid inhibited the enzyme activity.
    Analysis of Volatile compouds from Maitake mushroom (Grifola frondosa) homogenate was done by gaschromatograph equipped with PEG 20M fused silica capilary column (i.d.0.25 mm x 60 m).
    The capacity of maitgake lipoxygenase and hydroperoxidelyase to generate short-chaine volatile carbonyl compounds depended on the concentrations of enzyme, substrate fatty acids, and duration of incubation period. The major volatile compounds generated from the oxidation of linoleic acid by lipoxygenase and hydroperoxide lyase in maitake homogenate were 1-octen-3-ol (60%), 3-octenol (31%), 2-octenal (3%), n-heptanal (2.2%), methyl cinnamate (1.5%), nerolidol (1.3%), 2-dcanone (0.8%), n-octanal (0.4%), 3-octen-3one, and etc.
    The molecular weight of lipoxigenase from Hiratake mushroom (Pleurotuso streatus), was 87.00 by the use of gel chromatography with Sephacryl S-400.

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  • イネ培養細胞におけるゴルジ複合体膜タンパク質の局在化機構

    Grant number：05760065

    1993 - 1994

    System name：科学研究費助成事業

    Research category：奨励研究(A)

    Awarding organization：日本学術振興会

    三ツ井 敏明

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    Grant amount：\900000 （ Direct Cost: \900000 ）

    イネ培養細胞におけるゴルジ複合体構成膜タンパク質の局在化のメカニズムを解明するために、平成5年度は以下のような研究を行った。
    1.ゴルジ体膜からのピ-ナッツレクチン認識糖タンパク質(PNA-GP)の精製:グリセロール密度勾配遠心分離法を用いてイネ培養細胞のミクロソーム膜画分よりゴルジ体膜を単離し、SDS-PAGEによって31kDa、29kDa、23kDa、及び21kDaのPNA-GPを分離、精製した。21kDaのPNA-GPに対するポリクローナル抗体を作成し、その特異性を調べたところ、1M NaC1存在下においては23kDaと21kDaを、0.5M methy1-alpha-mannoside存在下においては21kDa PNA-GPを特異的に認識することが分かった。この結果は、23kDaと21kDa PNA-GPのN-結合型糖鎖の構造は類似しているが、ポリペプチド鎖は異なることを示している。2.PNA-GPの糖鎖構造と生合成に関する検討:PNA-GPは、PNAによって認識され、アルカリ処理及びO-グルカナーゼ処理によってPNA認識糖鎖の切断が起こることから、PNA-GPはO-結合型糖鎖を有していることが強く示唆された。さらに、PNA-GPの生成及び局在化に及ぼすBrefeldin A、Monensin、及びTunicamycinの影響を調べたところ、Brefeldin Aによってそれらは阻害されるが、MonensinとTunicamycinでは阻害は見られなかった。この結果から、このO-結合糖鎖はゴルジ複合体のシスコンパートメントで生成が始まることが示唆された(PCP(1993)34,855-863)。3.PNA-GPの遺伝子構造に関する検討:イネゴルジ複合体構成膜タンパク質の中で比較的含量の多いPNA-GPのポリペプチド鎖を特異的に認識する抗体が調製できたので、PNA-GPのcDNAクローンの調製及び構造決定を現在進めている。

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  • DYNAMICS OF CELLULAR FUNCTION OF PLANT VACUOLES.

    Grant number：05304005

    1993 - 1994

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Co-operative Research (A)

    Awarding organization：Japan Society for the Promotion of Science

    MAESHIMA Masayoshi, MITSUI Toshiaki, NAKAMURA Kenzo, NISHIMURA Ikuko, OHSUMI Yoshinori, FUTAI Masamitsu

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    Grant amount：\18900000 （ Direct Cost: \18900000 ）

    Vacuole is one on the most conspicuous compartments within a plant cell. We have investigated dynamic aspect of plant vacuoles in function and morphology. The vacuole performs numerous functions vital to cellular homeostasis, including the accumulation of metabolites, storage proteins and inorganic ions. The vacuolar function is supported by the primary and secondary active transport systems. We focused our attention on the vacuolar proton pumps which acidify vacuoles and create a membrane potential. Also, Na^+/H^+antiporter has been characterized as the secondary active transporter. The most important aspect of vacuoles is their role in protein storage. The protein storage vacuoles are derived from central vacuoles in the maturating seeds and transformed to a central vacuole in the germinating seeds. We analyzed the mechanisms for the protein accumulation into these protein-storage vacuoles and for the transformation between the protein-storage vacuole and the central vacuole. And the mechanisms of vacuolar morphogenesis and the molecular basis of autophagic function have been investigated for yeast and plant cells.
    Our investigations revealed the physiological significance of vacuolar proton pumps in the ion accumulation, intracellular sorting of storage protein to vacuole and cell expansion. Nishimura's group found the dense vesicles that act as transport vesicles from ER to vacuoles for storage proteins in seeds. In yeast systems, the essential genes and protein factor for vacuole morphogenesis have been identified. Ohsumi's group found the autophagy in yeast cells and analyzed the process at levels of cell biology and molecular genetics. During this study, some fruitful, cooperative works among the members have been done.

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  • イネ培養細胞におけるゴルジ複合体膜結合型蛋白質の局在化機構

    Grant number：01760064

    1989

    System name：科学研究費助成事業

    Research category：奨励研究(A)

    Awarding organization：日本学術振興会

    三ツ井 敏明

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    Grant amount：\1000000 （ Direct Cost: \1000000 ）

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  • Activity expression of L-myo-inositol-1-phosphate synthase in suspension-cultured rice cells

    Grant number：63560077

    1988 - 1989

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for General Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    IGAUE Ikuo, MITSUI Toshiaki

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    Grant amount：\1900000 （ Direct Cost: \1900000 ）

    Activity expression, activity change, enzyme purification and properties of L-myo-inositol- 1-phosphate synthase(MIPS) were analyzed employing rice cells in suspension-culture. myo-Inositol(MI) biosynthesis and a role in controlling metabolism also were investigated. It was shown that in MI deficient medium at the early logarithmic stage, the activity of MIPS is prominent, but in medium to which MI was, is suppressed. Sigmoid curve of the enzyme activity with critical point, 20 mg MI/1, was observed. Furthermore, the activity change at MI concentration was examined, Km was unchanged but Vmax varied in proportion to the activity. It was, therefore, suggested that the activity regulation by MI was not inhibition of enzyme activity but synthetic regulation of enzyme molecules.
    Using 20 mM Tris-HC1 buffer(pH 7.5) containing 20%(V/V) glycerin, 2 mM 2-mercaptoethanol, the purification of enzyme was performed at 3ﾟC. The enzyme was isolated from the cell extract and purified 600-fold with a 6% yield by ammonium sulfate fractional precipitation, and chromatographies on DEAE-Toyopearl, butyl-Toyopearl, Hydroxylapatite, Cellulofine GCL-2000 and chromatofocusing Attempts to obtain the homogeneous enzyme were unsuccessful.
    On the other hand, due to the presence of the powerful non-specific acid phosphatase in the enzyme, periodate oxidation-malachite green method was adopted instead of bioassay method, and subsequent assay was simplified. NH^+_ accelerates the rate of reaction 4-fold. The enzyme showed pH optimum at 8.0 with Km value of 0.79 mM. The activity was about 15 - 16% NAD^+-independent. The activity was remarkably inhibited by a number of pentose phosphate pathway: 6-phosphogluconate, riburose-5-phosphate, ribose-5-phosphate and fructose-6-phospha-Le. The enzyme activity was inhibited by Ag^+, Cu^<2+>, Zn^<2+>, Ba^<2+> and Fe^<3+>. Some sulfhydryl reagent and nucleotide-coenzyme used also were inhibitory.

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Teaching Experience

  • 日本酒学概論I(自然科学)

    2023
    Institution name：新潟大学

  • スタディ・スキルズAIIb

    2022
    -
    2023
    Institution name：新潟大学

  • スタディ・スキルズAIb

    2022
    -
    2023
    Institution name：新潟大学

  • 統合化学入門

    2022
    Institution name：新潟大学

  • Topics in Biotechnology and Biochemistry

    2022
    Institution name：新潟大学

  • 応用生命・食品科学セミナーIV

    2022
    Institution name：新潟大学

  • 実践型食づくりプロジェクト

    2021
    -
    2023
    Institution name：新潟大学

  • 自然科学総論IV

    2021
    Institution name：新潟大学

  • 農学入門I

    2021
    Institution name：新潟大学

  • 農学入門II

    2021
    Institution name：新潟大学

  • 植物バイオコントロール特論

    2021
    Institution name：新潟大学

  • 食品科学概論

    2020
    -
    2021
    Institution name：新潟大学

  • 領域概説 F (農学)

    2020
    Institution name：新潟大学

  • スタディ・スキルズA b

    2020
    Institution name：新潟大学

  • 植物分子生命科学特論

    2020
    Institution name：新潟大学

  • 応用生命科学セミナー

    2020
    Institution name：新潟大学

  • 生命を知る

    2018
    -
    2023
    Institution name：新潟大学

  • Topics in Biotechnology and Biochemistry

    2018
    -
    2022
    Institution name：新潟大学

  • 基礎化学

    2017
    -
    2023
    Institution name：新潟大学

  • 実践型食づくりプロジェクト

    2015
    -
    2023
    Institution name：新潟大学

  • 応用生命・食品科学概論

    2015
    -
    2018
    Institution name：新潟大学

  • 応用生命・食品科学特論

    2015
    -
    2018
    Institution name：新潟大学

  • Research Agli-Internships

    2015
    Institution name：新潟大学

  • Practical English

    2015
    Institution name：新潟大学

  • 外国語論文解説・討論Ⅰ

    2015
    Institution name：新潟大学

  • 生命・食料科学博士特定研究Ⅲ

    2014
    Institution name：新潟大学

  • 生命・食料科学博士セミナーⅢ

    2014
    Institution name：新潟大学

  • 研究発表演習(中間発表)

    2013
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学特定研究BⅡ

    2013
    -
    2015
    Institution name：新潟大学

  • 文献詳読Ⅱ

    2013
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学特定研究BⅠ

    2013
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学セミナーBⅡ

    2013
    -
    2015
    Institution name：新潟大学

  • 文献詳読Ⅰ

    2013
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学博士特定研究Ⅱ

    2013
    -
    2015
    Institution name：新潟大学

  • 応用生命・食品科学演習(学会発表)

    2013
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学セミナーBⅠ

    2013
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学博士セミナーⅡ

    2013
    Institution name：新潟大学

  • 生命・食料科学博士特定研究Ⅰ

    2012
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学博士セミナーⅠ

    2012
    -
    2015
    Institution name：新潟大学

  • 研究発表

    2012
    Institution name：新潟大学

  • 酵素化学

    2011
    -
    2024
    Institution name：新潟大学

  • 大型機器分析技術

    2011
    -
    2020
    Institution name：新潟大学

  • 分子生命科学演習Ⅰ

    2011
    -
    2019
    Institution name：新潟大学

  • 分子生命科学演習Ⅱ

    2011
    -
    2018
    Institution name：新潟大学

  • 分子生命科学実験

    2010
    -
    2018
    Institution name：新潟大学

  • スタディ・スキルズA2

    2010
    -
    2016
    Institution name：新潟大学

  • 生物化学Ⅰ

    2008
    Institution name：新潟大学

  • 植物バイオコントロール学

    2008
    -
    2020
    Institution name：新潟大学

  • 生物化学Ⅱ

    2008
    -
    2017
    Institution name：新潟大学

  • バイオインフォマテイクス概論

    2008
    Institution name：新潟大学

  • 植物ゲノム機能学特論

    2008
    Institution name：新潟大学

  • 生物化学実験

    2007
    -
    2023
    Institution name：新潟大学

  • 植物代謝制御特論

    2007
    -
    2023
    Institution name：新潟大学

  • 植物生化学

    2007
    -
    2023
    Institution name：新潟大学

  • 植物バイオコントロール特論

    2007
    -
    2021
    Institution name：新潟大学

  • 生命と環境の化学 I

    2007
    -
    2010
    Institution name：新潟大学

  • 化学

    2007
    -
    2009
    Institution name：新潟大学

  • 植物ゲノム科学

    2007
    -
    2008
    Institution name：新潟大学

  • バイオインフォマテイクス特論

    2007
    -
    2008
    Institution name：新潟大学

  • 生物化学I

    2007
    Institution name：新潟大学

  • 生物化学II

    2007
    Institution name：新潟大学

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Media Coverage

  • 「食の新潟国際賞」6人受賞“暑さに強いコメ”新大教授も

    TeNY新潟一番  2024.11

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  • 本学社会連携推進機構の三ツ井敏明特任教授が第８回食の新潟国際賞地域未来賞を受賞しました

    本学社会連携推進機構の三ツ井敏明特任教授が第８回食の新潟国際賞地域未来賞を受賞しました  2024.11

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  • 本学教員2名が第77回新潟日報文化賞を受賞し ました。

    新潟大学トピックス  2024.11

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  • 新潟県の文化や産業の発展に貢献「佐渡を世界遺産にする会」など1団体と4人に『新潟日報文化賞』

    BSN新潟放送  2024.11

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  • 第77回新潟日報文化賞 産業技術部門 新潟大学特任教授三ツ井敏明氏 コメの白濁化の仕組み解明

    新潟日報  2024.10

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  • コメ共創IPベトナムで栽培開発」「NU1号「新大コシヒカリ」農業救う新品種開発

    読売新聞  2024.10

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  • 塩害にも強い「新大コシ」5年後実用化へ三ツ井教授研究

    新潟日報  2024.9

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  • 大規模な“塩害”乗り越え稲実る！去年高温少雨で海水混じった田んぼ…土壌の塩分濃度は問題なし

    NSTNEWS  2024.9

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  • 優れた研究・調査たたえ 食の新潟国際賞 大賞に2人

    新潟日報  2024.8

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  • 猛暑に負けない“新大コシヒカリ”300㎏を子ども食堂に寄付

    UXニュース  2024.8

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  • 新潟大学が夏の高温に耐えるコシヒカリを開発 「新大コシヒカリ」の生みの親三ツ井敏明博士を訪ねました。

    キャレル  2024.8

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  • 刈羽発 新大コシに注目 暑さに強い稲 研究進む

    新潟日報  2024.8

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  • おいしい米で元気に育って 新大、市に新品種コシ寄付

    新潟日報  2024.8

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  • 校外学習「新大コシヒカリを見てみよう、触れてみよう。」

    大鷲小学校  2024.7

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  • 「新大コシ」ってどんな米？ 南区・大鷲小学校が総合学習 三ツ井特任教授が解説 Newspaper, magazine

    新潟日報  2024.7

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  • 【第８回食の新潟国際賞】県内では別府茂氏が「２１世紀希望賞」新谷梨恵子氏と三ツ井敏明氏が「地域未来賞」を受賞

    にいがた経済新聞  2024.7

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  • 学級活動「プランターお米作り体験「新大コシヒカリとは？」」

    新大付属幼稚園  2024.6

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  • 去年の被害大きく…“塩 害に強い”コメ作付け！気候変動に負けない農業を「実り・収量に期待」

    FNNプライムオンライン  NST新潟総合テレビ  2024.5

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  • 「4日連続の雨がほしい」コメ農家は深刻な水不足に苦悩…各地で真夏日100地点越え TV or radio program

    テレビ朝日  報道ステーション  2024.5

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  • コメどころで研究20年 暑さに強いコシヒカリ開発 Promotional material

    広報ねやがわ  2024.5

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  • 猛暑に悩む新潟のコメ 耐高温品種への転換阻む「水問題」 Newspaper, magazine

    日本経済新聞  2024.3

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  • 三ツ井敏明教授、村政功労者表彰 Promotional material

    広報かりわ2月号  2024.2

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  • 猛暑の影響をうけない 安定的に収穫できる農作物 TV or radio program

    日本テレビ  ZIP  2024.1

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  • 「新大コシヒカリ」は猛暑に強い新潟大の開発品種に脚光 多彩人材三ツ井敏明教授 Newspaper, magazine

    日本経済新聞電子版  2023.12

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  • 「コシヒカリ、猛暑に勝て 23年度産「新大コシヒカリ」従来品種上回る品質に」 Newspaper, magazine

    日本経済新聞  2023.12

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  • 新大コシヒカリ栽培実験4年目 TV or radio program

    新潟日報  2023.12

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  • 猛暑で田んぼが枯れて茶色に…“塩分7倍”の田んぼ3カ月経った”土壌”のいま TV or radio program

    NST  Newsタッチ  2023.11

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  • 地球温暖化のなかの米づくり 気候変動に地域社会で取り組む高温耐性「新大コシヒカリ」 Newspaper, magazine

    農業協同組合新聞  2023.10

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  • なぜ？魚沼産コシヒカリが灰色に「農家初めて最悪の年」”新潟一丸”で猛暑に負けないコシヒカリ開発の挑戦 TV or radio program

    TBS  Nスタ  2023.10

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  • 新潟大学が開発した暑さに強いコシヒカリの新品種の新しい名前は？ Newspaper, magazine

    新潟日報  2023.10

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  • 高温で農作物品質低下 気候変動どう備える TV or radio program

    NHK総合  2023.10

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  • コシヒカリ進む品種改良 TV or radio program

    テレビ朝日  羽鳥慎一モーニングショー  2023.10

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  • 暑さに強いコメづくりを Newspaper, magazine

    新潟日報  2023.10

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  • 命名「新大コシヒカリ」教授ら開発、暑さに強く Newspaper, magazine

    読売新聞  2023.9

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  • “米どころ”温暖化からいかに守るか TV or radio program

    NHK総合  新潟610  2023.9

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  • 今週の新潟で何が起こった⁉ TV or radio program

    UX新潟テレビ  まるどり！UP  2023.9

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  • 暑さに強いコシヒカリを コメどころ新潟を襲う猛暑 コシヒカリの品種改良が続く TV or radio program

    TeNY  2023.9

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  • 田んぼに海水 塩害か Newspaper, magazine

    新潟日報  2023.9

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  • 田んぼが茶色に「コシヒカリ」塩害か TV or radio program

    フジテレビ  めざまし８  2023.9

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  • 「このコメは食べられない」なぜ？田んぼが茶色に… 原因は渇水による“塩害”土壌から7倍の塩分検出 TV or radio program

    NST新潟総合テレビ  2023.8

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  • 渇水、塩害 TV or radio program

    BSN新潟放送  ゆうなび  2023.8

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  • 農業のシンカを探る TV or radio program

    UX新潟テレビ21  スーパーJにいがた第3部  2023.8

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  • コシ「NU1号」開発の新大 メタン抑制農法に着手 AIカメラ、センサー設置 稲の生育状況 水温数値化 Newspaper, magazine

    新潟日報  2023.6

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  • 暑さに強いコシNU1号中干し延長環境優しく刈羽村のほ場 水田センサーなど設置 Newspaper, magazine

    柏崎日報  2023.5

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  • 安全規則から災害対策まで 新たな学問確率目指す Newspaper, magazine

    新潟日報  2023.4

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  • 新大開発コシ「NU1号」栽培3年目「整粒」割合の高さ維持一般流通に向け名称募集 Newspaper, magazine

    新潟日報  2022.11

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  • 刈羽中「NU１号」の新米給食 開発者三ツ井教授も Newspaper, magazine

    柏崎日報  2022.11

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  • 暑さに強いコシヒカリとして新潟大学が開発した新品種米 コメのネーミングを募集 TV or radio program

    TeNYテレビ新潟  2022.11

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  • おいしさと、環境へ心配りと 味・手間そのまま 暑さに強い品種開発 Newspaper, magazine

    朝日小学生新聞  2022.11

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  • 新潟のブランド米へ 暑さに強いコメ「ＮＵ1号」の開発 実証実験3年目 ことしの出来栄えは

    TeNYテレビ新潟  新潟一番  2022.11

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  • 新潟大開発のコメ、栽培地域拡大

    日本経済新聞  2022.11

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  • コシヒカリ新潟大学NU1号 高温耐性コシ実証報告 今年も品質良好

    日本農業新聞  2022.10

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  • 新潟大学が高温耐性などを持つ新品種「コシヒカリ新潟大学NU1号」の実証実験の結果を報告

    にいがた経済新聞  2022.10

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  • 暑さに強いコシヒカリの新品種は高品質 新潟大が名称募集へ TV or radio program

    NHK  新潟ニュース610  2022.10

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  • 暑さに強いコシヒカリ「新潟大学NU1号」2年目の田植え

    UX新潟テレビ21  スーパーJにいがた  2021.5

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  • NU1号味わってね 新大研究センター 刈羽小・刈羽中に寄

    柏崎日報  2021.3

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  • 暑さに負けぬコシへ「Nu１号」開発者招き研修会 新潟・岩船地域生産協

    日本農業新聞  2021.3

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  • 新潟大学、新品種コシNU1号を子ども食堂へ寄付

    ＵＸ新潟テレビ21  2021.2

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  • 新品種コシ子ども食堂へ 新大、市社協に100キロ寄付

    新潟日報  2021.2

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  • そのコメはうまいか ～“越の国に光れ”願い紡いで～

    ＴｅＮＹテレビ新潟  2020.12

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  • 新潟大学が来年度実証実験に向けて応援基金『コシヒカリ新潟大学NU1号応援基金』を創設

    にいがた経済新聞  2020.12

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  • 新品種『コシヒカリ新潟大学NU1号』暑さに耐性試験栽培で確認

    朝日新聞  2020.11

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  • 暑さに強いコメ実証 新大開発『ＮＵ1号』刈羽で試食会 味も高評価

    新潟日報  2020.11

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  • 新品種『NU1号』披露 品質や食味に関心

    柏崎日報  2020.11

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  • 暑さに強い『コシヒカリ新潟大学NU1号』農業関係者にお披露目

    ＴｅＮＹテレビ新潟  2020.11

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  • 新大開発『コシNU1号』暑さへの強さ実証

    新潟日報  2020.10

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  • 新潟大発のコメ新品種 暑さへの耐性を確認

    日本経済新聞電子版  2020.10

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  • 新潟大NU1号試験−高温耐性コシ手応え

    日本農業新聞  2020.10

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  • 新潟大学が暑さに強いコシヒカリ新品種を開発

    にいがた経済新聞  2020.10

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  • 暑さに強いコシヒカリ開発

    ＮＨＫ  新潟ニュース６１０  2020.10

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  • 気候変動に向き合うコシヒカリの未来は？

    ＴｅＮＹテレビ新潟  新潟一番  2020.10

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  • 暑さに強い新コシヒカリ

    中国新聞他3社  2020.10

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  • 暑さに強い新コシヒカリ−ブランド維持に期待

    共同通信社  2020.10

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  • 改良コシ生育は順調 新大開発、刈羽で稲刈

    新潟日報  2020.9

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  • コシヒカリ新潟大学NU1号

    柏崎日報  柏崎抄  2020.9

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  • 暑さに強い！新品種『コシ』『NU1号』黄金色の穂 新大と刈羽村期待こめ収穫

    柏崎日報  2020.9

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  • “NU1号”初収穫！1年目の出来は…

    新潟総合テレビ  ＮＳＴNewsタッチ  2020.9

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