2021/10/20 更新

写真a

イノウエ カヨコ
井上 佳世子
INOUE Kayoko (NOZAWA-INOUE Kayoko)
所属
教育研究院 医歯学系 特任准教授
医歯学総合研究科 特任准教授
職名
特任准教授
通称等の別名
野澤-井上 佳世子
外部リンク

学位

  • 博士(歯学) ( 1998年3月   新潟大学 )

研究キーワード

  • 口腔外科学

  • 口腔組織学

  • Oral histology

  • Oral and maxillofacial surgery

研究分野

  • ライフサイエンス / 外科系歯学

  • ライフサイエンス / 常態系口腔科学

経歴(researchmap)

  • :滑膜表層B型細胞におけるカベオラの存在意義に関する研究に従事.

    2004年 - 現在

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  • :ラット顎関節の発生,および滑膜表層細胞におけるストレス応答経路に関する研究に従事.

    2003年 - 現在

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  • :ラット顎関節滑膜におけるエストロゲン受容体の発現に関する免疫細胞化学的研究に従事.

    2001年 - 2003年

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  • :英国レスター大学にて免疫細胞化学を用いた形態学的研究に従事.

    1999年 - 2001年

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  • :ラットおよびヒト顎関節滑膜における熱ショックタンパクの発現に関する免疫細胞化学的研究に従事.

    1998年 - 現在

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  • :正常ラット顎関節滑膜における細胞結合に関する免疫細胞化学的研究に従事.

    1997年 - 現在

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  • :ラット顎関節炎モデルにおける滑膜の病理組織学的変化の観察および免疫細胞化学的研究に従事.

    1994年 - 現在

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▶ 全件表示

経歴

  • 新潟大学   医歯学総合研究科   特任准教授

    2009年1月 - 現在

  • 新潟大学   医歯学総合研究科 口腔生命科学専攻 摂食環境制御学   准教授

    2006年4月 - 2008年12月

  • 新潟大学   医歯学総合研究科 口腔生命科学専攻 摂食環境制御学   助手

    2001年4月 - 2006年3月

  • 新潟大学   歯学部 歯学科   助手

    1998年4月 - 2001年3月

学歴

  • 新潟大学   歯学研究科   歯学臨床系

    - 1998年

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    国名: 日本国

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  • 新潟大学

    - 1998年

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  • 新潟大学

    - 1994年

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  • 新潟大学   歯学部   歯学

    - 1994年

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    国名: 日本国

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所属学協会

取得資格

  • 歯科医師

 

論文

  • Differential response properties of peripherally and cortically evoked swallows by electrical stimulation in anesthetized rats 査読

    Takanori Tsujimura, Kojun Tsuji, Jin Magara, Shogo Sakai, Taku Suzuki, Yuki Nakamura, Kayoko Nozawa-Inoue, Makoto Inoue

    BRAIN RESEARCH BULLETIN   122   12 - 18   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    We compared onset latency, motor-response patterns, and the effect of electrical stimulation of the cortical masticatory area between peripherally and cortically evoked swallows by electrical stimulation in anesthetized rats. The number of swallows and the motor patterns were determined using electromyographic recordings from the thyrohyoid, digastric, and masseter muscles. The onset latency of the first swallow evoked by electrical stimulation of the cortical swallowing area (Cx) was significantly longer than that evoked by stimulation of the superior laryngeal nerve (SLN). The duration of thyrohyoid burst activity associated with SLN-evoked swallows was significantly longer than that associated with either Cx-evoked or spontaneous swallows. Combining Cx with SLN stimulation increased the number of swallows at low levels of SLN stimulation. Finally, A-area (the orofacial motor cortex) stimulation inhibited Cx-evoked swallows significantly more than it inhibited SLN-evoked swallows. These findings suggest that peripherally and cortically evoked swallows have different response properties and are affected differently by the mastication network. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.brainresbull.2016.02.015

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  • Contribution of synovial lining cells to synovial vascularization of the rat temporomandibular joint 査読

    Kayoko Nozawa-Inoue, Fumiko Harada, Jin Magara, Atsushi Ohazama, Takeyasu Maeda

    JOURNAL OF ANATOMY   228 ( 3 )   520 - 529   2016年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The lining layer of the synovial membrane in the temporomandibular joint (TMJ) contains two types of lining cells: macrophage-like type A and fibroblast-like type B cells. The type B cells are particularly heterogeneous in their morphology and immunoreactivity, so that details of their functions remain unclear. Some of the type B cells exhibit certain resemblances in their ultrastructure to those of an activated capillary pericyte at the initial stage of the angiogenesis. The articular surface, composed of cartilage and the disc in the TMJ, has few vasculatures, whereas the synovial lining layer is richly equipped with blood capillaries to produce the constituent of synovial fluid. The present study investigated at both the light and electron microscopic levels the immunocytochemical characteristics of the synovial lining cells in the adult rat TMJ, focusing on their contribution to the synovial vascularization. It also employed an intravascular perfusion with Lycopersicon esculentum (tomato) lectin to identify functional vessels in vivo. Results showed that several type B cells expressed desmin, a muscle-specific intermediate filament which is known as the earliest protein to appear during myogenesis as well as being a marker for the immature capillary pericyte. These desmin-positive type B cells showed immunoreactions for vimentin and pericyte markers (neuron-glial 2; NG2 and PDGFRb) but not for the other markers of myogenic cells (MyoD and myogenin) or a contractile apparatus (aSMA and caldesmon). Immunoreactivity for RECA-1, an endothelial marker, was observed in the macrophage-like type A cells. The arterioles and venules inside the synovial folds extended numerous capillaries with RECA-1-positive endothelial cells and desmin-positive pericytes to distribute densely in the lining layer. The distal portion of these capillaries showing RECA-1-immunoreactivity lacked lectin-staining, indicating a loss of blood-circulation due to sprouting or termination in the lining layer. The desmin-positive type B and RECA-1-positive type A cells attached to this portion of the capillaries. Some capillaries in the lining layer also expressed ninein, a marker for sprouting endothelial cells, called tip cells. Since an activated pericyte, macrophage and tip cell are known to act together at the forefront of the vessel sprout during angiogenesis, the desmin-positive type B cell and RECA-1-positive type A cell might serve as these angiogenic cells in the synovial lining layer. Tomato lectin perfusion following decalcification would be a highly useful tool for research on the vasculature of the mineralized tissue. Use of this technique combined with immunohistochemistry should permit future extensive investigations on the presence of the physiological angiogenesis and on the function of the lining cells in the synovial membrane. Key words: angiogenesis; synovial lining cell; synovial membrane; temporomandibular joint; tomato lectin.

    DOI: 10.1111/joa.12426

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  • Excess NF-κB induces ectopic odontogenesis in embryonic incisor epithelium. 査読

    Blackburn J, Kawasaki K, Porntaveetus T, Kawasaki M, Otsuka-Tanaka Y, Miake Y, Ota MS, Watanabe M, Hishinuma M, Nomoto T, Oommen S, Ghafoor S, Harada F, Nozawa-Inoue K, Maeda T, Peterková R, Lesot H, Inoue J, Akiyama T, Schmidt-Ullrich R, Liu B, Hu Y, Page A, Ramírez Á, Sharpe PT, Ohazama A

    Journal of dental research   94 ( 1 )   121 - 128   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/0022034514556707

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  • A histologic study of deformation of the mandibular condyle caused by distraction in a rat model 査読

    Naoko Sakagami, Tadaharu Kobayashi, Kayoko Nozawa-Inoue, Kimimitsu Oda, Taku Kojima, Takeyasu Maeda, Chikara Saito

    ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY   118 ( 3 )   284 - 294   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Objective. This study investigated the bone resorption process of the rat mandibular condyle after mandibular distraction.
    Study Design. Male Wistar rats at 10 weeks of age underwent unilateral mandibular distraction at 0.175 mm per 12 hours for 10 days. Histologic and histochemical analyses were performed at postoperative day 1 and weeks 1 and 3.
    Results. High-resolution computed tomography (micro-CT) observations showed that deformation of the condyle occurred in the anterior region, where a discontinuity of the condylar cartilage layer was found in histologic sections. This destroyed area gathered many osteoclasts. In the central region, disorganization with a thin hypertrophic cell layer was recognizable by day 1 but later thickened. Morphologic recovery of the mandibular condyle could be attained by week 3 in this animal model.
    Conclusions. These morphologic findings indicate that rapid deformation of the condyle, with destruction of the cartilage layer and bone resorption, was caused by artificial distraction.

    DOI: 10.1016/j.0000.2014.05.003

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  • Zoledronic acid impairs re-epithelialization through down-regulation of integrin αvβ6 and transforming growth factor beta signalling in a three-dimensional in vitro wound healing model. 査読

    Saito T, Izumi K, Shiomi A, Uenoyama A, Ohnuki H, Kato H, Terada M, Nozawa-Inoue K, Kawano Y, Takagi R, Maeda T

    International journal of oral and maxillofacial surgery   43 ( 3 )   373 - 380   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.ijom.2013.06.016

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  • Distinct expression patterns and roles of aldehyde dehydrogenases in normal oral mucosa keratinocytes: differential inhibitory effects of a pharmacological inhibitor and RNAi-mediated knockdown on cellular phenotype and epithelial morphology 査読

    Hiroko Kato, Kenji Izumi, Taro Saito, Hisashi Ohnuki, Michiko Terada, Yoshiro Kawano, Kayoko Nozawa-Inoue, Chikara Saito, Takeyasu Maeda

    HISTOCHEMISTRY AND CELL BIOLOGY   139 ( 6 )   847 - 862   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Aldehyde dehydrogenases (ALDHs), enzymes responsible for detoxification and retinoic acid biosynthesis, are considered a potent functional stem cell marker of normal and malignant cells in many tissues. To date, however, there are no available data on ALDH distributions and functions in oral mucosa. This study aims to clarify the levels and types of ALDH expression using immunohistochemistry with accompanying mRNA expression as well as an ALDEFLUOR assay, and to assess phenotypic and histological changes after manipulation of the ALDH activity of oral keratinocytes to increase the potency of a tissue-engineered oral mucosa by a specific ALDH inhibitor, diethylaminobenzaldehyde (DEAB), together with small interfering RNA of ALDH1A3 and ALDH3A1. Results showed the mRNA and cytoplasmic protein expression of ALDH1A3 and ALDH3A1 to be mostly localized in the upper suprabasal layer although no ALDH1A1 immunoreaction was detected throughout the epithelium. Oral keratinocytes with high ALDH activity exhibited a profile of differentiating cells. By pharmacological inhibition, the phenotypic analysis revealed the proliferating cell-population shifting to a more quiescent state compared with untreated cells. Furthermore, a well-structured epithelial layer showing a normal differentiation pattern and a decrease in Ki-67 immunopositive basal cells was developed by DEAB incubation, suggesting a slower turnover rate efficient to maintain undifferentiated cells. Histological findings of a regenerated oral epithelium by ALDH1A3 siRNA were similar to those when treated with DEAB while ALDH3A1 siRNA eradicated the epithelial regenerative capacity. These observations suggest the effects of phenotypic and morphological alterations by DEAB on oral keratinocytes are mainly consequent to the inhibition of ALDH1A3 activity.

    DOI: 10.1007/s00418-012-1064-7

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  • Construction and characterization of a tissue-engineered oral mucosa equivalent based on a chitosan-fish scale collagen composite 査読

    Michiko Terada, Kenji Izumi, Hisashi Ohnuki, Taro Saito, Hiroko Kato, Marie Yamamoto, Yoshiro Kawano, Kayoko Nozawa-Inoue, Haruhiko Kashiwazaki, Toshiyuki Ikoma, Junzo Tanaka, Takeyasu Maeda

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS   100B ( 7 )   1792 - 1802   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    This study was designed to (1) assess the in vitro biocompatibility of a chitosancollagen composite scaffold (C3) constructed by blending commercial chitosan and tilapia scale collagen with oral mucosa keratinocytes, (2) histologically and immunohistochemically characterize an ex vivo-produced oral mucosa equivalent constructed using the C3 (EVPOME-C), and (3) compare EVPOME-C with oral mucosa constructs utilizing AlloDerm (R) (EVPOME-A), BioMend (R) Extend (TM) (EVPOME-B), and native oral mucosa. C3 scaffold had a well-developed fibril network and a sufficiently small porosity to prevent keratinocytes from growing inside the scaffold after cell-seeding. The EVPOME oral mucosa constructs were fabricated in a chemically defined culture system. After culture at an air-liquid interface, EVPOME-C and EVPOME-B had multilayered epithelium with keratinization, while EVPOME-A had a more organized stratified epithelium. Ki-67 and p63 immunolabeled cells in the basal layer of all EVPOMEs suggested a regenerative ability. Compared with native oral mucosa, the keratin 15 and 10/13 expression patterns in all EVPOMEs showed a less-organized differentiation pattern. In contrast to the beta 1-integrin and laminin distribution in EVPOME-A and native oral mucosa, the subcellular deposition in EVPOME-C and EVPOME-B indicated that complete basement membrane formation failed. These findings demonstrated that C3 has a potential application for epithelial tissue engineering and provides a new potential therapeutic device for oral mucosa regenerative medicine. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.

    DOI: 10.1002/jbm.b.32746

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  • Immunoexpression of aquaporin-1 in the rat periodontal ligament during experimental tooth movement 査読

    Tomoko Yoshii, Fumiko Harada, Isao Saito, Kayoko Nozawa-Inoue, Yoshiro Kawano, Takeyasu Maeda

    BIOMEDICAL RESEARCH-TOKYO   33 ( 4 )   225 - 233   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    This study examined the immunoexpression pattern of aquaporin-1 (AQP1), first identified as a water channel protein, in the periodontal ligament of rat molars during experimental tooth movement to clarify its role in periodontal responses in an overloaded model by the insertion of a piece of elastic band. In the control group without any treatment, the cementoblasts and osteogenic cells as well as the vascular endothelial cells showed AQP1 immunoreaction. In the experimental group, hyalinized tissue and intensely AQP1 positive amorphous structures which were identified as degenerated endothelial cells by immunoelectron microscopy, occurred at the compression side on Days 1 and 3. AQP1 immunoreaction came to be stronger in the intact endothelial cells around the hyalinized tissue. The hyalinized tissue had almost disappeared by Day 5 when many macrophages reactive to acid phosphatase activity appeared. The periodontal width on Day 7 became almost the same as that in the control group. These findings indicate that the hyalinized tissue and damaged AQP1 positive endothelial cells are phagocytized by macrophages which have temporally migrated, and suggest that the surviving endothelial cells with intense AQP1 reaction are involved in periodontal regeneration by capillary sprouting.

    DOI: 10.2220/biomedres.33.225

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  • Zoledronic acid induces S-phase arrest via a DNA damage response in normal human oral keratinocytes 査読

    Hisashi Ohnuki, Kenji Izumi, Michiko Terada, Taro Saito, Hiroko Kato, Akiko Suzuki, Yoshiro Kawano, Kayoko Nozawa-Inoue, Ritsuo Takagi, Takeyasu Maeda

    ARCHIVES OF ORAL BIOLOGY   57 ( 7 )   906 - 917   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: This study aimed to clarify the effects of zoledronic acid (ZOL) on human primary oral mucosal keratinocytes growing in a monolayer culture and on a tissue-engineered oral mucosal construct.
    Design: Changes in the viability and proliferation of oral keratinocytes incubated with ZOL were measured. Following treatment with 10 mu M ZOL, histological examinations and immunohistochemical analyses for Ki-67, Geminin, and phospho-histone (gamma)-H2A.X were performed on tissue-engineered oral mucosa. Cell cycle distribution and the degree of apoptosis were also measured by flow cytometry. Additionally, we measured the expression of cell cycle regulatory proteins as well as phospho-Chk1 and -Chk2.
    Results: ZOL treatment suppressed cell viability and proliferation in a dose-dependent manner. Compared with untreated tissue-engineered oral mucosa, ZOL treatment resulted in a thinner epithelium in which the basal cells appeared less-organised. This is consistent with the observed significant reduction in the Ki-67 labelling index. In contrast, the Geminin labelling index was significantly higher than that in the untreated sample. In spite of the presence of a few apoptotic cells, ZOL induced an arrest in S-phase, which was confirmed by our observed alterations in the expression levels of cyclin A, B1, p27(KIP1), Rb and phospho-Rb. When the proteasome inhibitor MG132 was added to the ZOL-treated cells, we observed a partial restoration of the expression of cyclin A, cyclin B1, and p27(KIP1). Expression of phospho-Chk1 was detected, and a significant increase in the labelling index of gamma-H2A.X was also seen.
    Conclusions: These results indicate that a 10-mu M ZOL treatment induces a DNA damage response in oral keratinocytes that activates the ubiguitin-mediated proteolysis of cell cycle regulators, resulting in cell cycle arrest and repressive effects on cell viability, proliferation, and epithelial turnover. (C) 2011 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.archoralbio.2011.11.015

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  • Alterations in intermediate filaments expression in disc cells from the rat temporomandibular joint following exposure to continuous compressive force 査読

    Jin Magara, Kayoko Nozawa-Inoue, Akiko Suzuki, Yoshiro Kawano, Kazuhiro Ono, Shuichi Nomura, Takeyasu Maeda

    JOURNAL OF ANATOMY   220 ( 6 )   612 - 621   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The articular disc in the temporomandibular joint (TMJ) that serves in load relief and stabilizing in jaw movements is a dense collagenous tissue consisting of extracellular matrices and disc cells. The various morphological configurations of the disc cells have given us diverse names, such as fibroblasts, chondrocyte-like cells and fibrochondrocytes; however, the characteristics of these cells have remained to be elucidated in detail. The disc cells have been reported to exhibit heterogeneous immunoreaction patterns for intermediate filaments including glial fibrillary acidic protein (GFAP), nestin and vimentin in the adult rat TMJ. Because these intermediate filaments accumulate in the disc cells as tooth eruption proceeds during postnatal development, it might be surmised that the expression of these intermediate filaments in the disc cells closely relates to mechanical stress. The present study was therefore undertaken to examine the effect of a continuous compressive force on the immunoexpression of these intermediate filaments and an additional intermediate filament muscle-specific desmin in the disc cells of the TMJ disc using a rat experimental model. The rats wore an appliance that exerts a continuous compressive load on the TMJ. The experimental period with the appliance was 5 days as determined by previous studies, after which some experimental animals were allowed to survive another 5 days after removal of the appliance. Histological observations demonstrated that the compressive force provoked a remarkable acellular region and a decrease in the thickness of the condylar cartilage of the mandible, and a sparse collagen fiber distribution in the articular disc. The articular disc showed a significant increase in the number of desmin-positive cells as compared with the controls. In contrast, immunopositive cells for GFAP, nestin and vimentin remained unchanged in number as well as intensity. At 5 days after removal of the appliance, both the disc and cartilage exhibited immunohistological and histological features in a recovery process. These findings indicate that the mature articular cells are capable of producing desmin instead of the other intermediate filaments against mechanical stress. The desmin-positive disc cells lacked a-smooth muscle actin (a-SMA) in this study, even though desmin usually co-exists with a-SMA in the vascular smooth muscle cells or pericytes. Because the precursor of a pericyte has such an immunoexpression pattern during angiogenesis, there is a further possibility that the formation of new vessels commenced in response to the extraordinary compressive force.

    DOI: 10.1111/j.1469-7580.2012.01501.x

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  • Phenotypes of articular disc cells in the rat temporomandibular joint as demonstrated by immunohistochemistry for nestin and GFAP 査読

    Hitoshi Miyako, Akiko Suzuki, Kayoko Nozawa-Inoue, Jin Magara, Yoshiro Kawano, Kazuhiro Ono, Takeyasu Maeda

    JOURNAL OF ANATOMY   219 ( 4 )   472 - 480   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The articular disc is a dense collagenous tissue containing disc cells that are phenotypically described as chondrocyte-like cells or fibrochondrocytes. Despite the possible existence of these phenotypes in systemic joints, little is known about the detailed classification of the articular disc cells in the temporomandibular joint. In this immunocytochemical study we examined the localization and distribution patterns of nestin and glial fibrillary acidic protein (GFAP) in the articular disc of the rat temporomandibular joint at postnatal day 1, and weeks 1, 2, 4 and 8, based on the status of tooth eruption and occlusion. Nestin and GFAP are intermediate filament proteins whose expression patterns are closely related to cell differentiation and cell migration. Both types of immunopositive cell greatly increased postnatally to a stable level after postnatal week 4, but they showed different distribution patterns and cell morphologies. Nestin-reactive disc cells, which were characterized by a meagre cytoplasm and thin cytoplasmic processes, were scattered in the articular disc, whereas GFAP-positive cells, characterized by broader processes, existed exclusively in the deeper area. In mature discs, the major proportion of articular disc cells exhibited GFAP immunoreactivity. Furthermore, a double-immunostaining demonstrated that the nestin-negative cells, consisting of GFAP-positive and -negative cells, exhibited immunoreactions for heat shock protein 25. These findings indicate that the articular disc cells comprise at least three types in the rat temporomandibular joint and suggest that their expressions closely relate to mechanical loading forces within the joint, including occlusal force, as observed through postnatal development.

    DOI: 10.1111/j.1469-7580.2011.01404.x

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  • A Morphological Analysis on the Osteocytic Lacunar Canalicular System in Bone Surrounding Dental Implants 査読

    Maiko Haga, Kayoko Nozawa-Inoue, Minqi Li, Kimimitsu Oda, Sumio Yoshie, Norio Amizuka, Takeyasu Maeda

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY   294 ( 6 )   1074 - 1082   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Osseointegration is the most preferable interface of dental implants and newly formed bone. However, the cavity preparation for dental implants often gives rise to empty lacunae or pyknotic osteocytes in bone surrounding the dental implant. This study aimed to examine the chronological alternation of osteocytes in the bone surrounding the titanium implants using a rat model. The distribution of the osteocytic lacunar canalicular system (OLCS) in bone around the titanium implants was examined by silver impregnation according to Bodian's staining. We also performed double staining for alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), as well as immunohistochemistry for fibroblast growth factor (FGF) 23-a regulator for the serum concentration of phosphorus-and sclerostin, which has been shown to inhibit osteoblastic activities. Newly formed bone and the injured bone at the early stage exhibited an irregularly distributed OLCS and a few osteocytes positive for sclerostin or FGF23, therefore indicating immature bone. Osteocytes in the surrounding bone from Day 20 to Month 2 came to reveal an intense immunoreactivity for sclerostin. Later on, the physiological bone remodeling gradually replaced such immature bone into a compact profile bearing a regularly arranged OLCS. As the bone was remodeled, FGF23 immunoreactivity became more intense, but sclerostin became less so in the well-arranged OLCS. In summary, it seems likely that OLCS in the bone surrounding the dental implants is damaged by cavity formation, but later gradually recovers as bone remodeling takes place, ultimately inducing mature bone. Anat Rec, 294:1074-1082, 2011. (C) 2011 Wiley-Liss, Inc.

    DOI: 10.1002/ar.21391

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  • Detection of acid-sensing ion channel 3 (ASIC3) in periodontal Ruffini endings of mouse incisors 査読

    Farhana Rahman, Fumiko Harada, Isao Saito, Akiko Suzuki, Yoshiro Kawano, Kenji Izumi, Kayoko Nozawa-Inoue, Takeyasu Maeda

    NEUROSCIENCE LETTERS   488 ( 2 )   173 - 177   2011年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    The acid-sensing ion channel 3 (ASIC3), a member of the epithelial sodium channel/degenerin (ENaC/DEG) superfamily, has been reported to participate in acid sensing, mechanosensation, and nociception. However, no information is available regarding the precise localization and function of this molecule in the periodontal ligament, which contains abundant sensory nerves originating from the trigeminal ganglion. The present study examined the expression of ASIC3 in the lingual periodontal ligament of mouse incisors by immunohistochemistry. Furthermore, the expression of ASIC3 in the trigeminal ganglion which innervates the periodontal ligament - was investigated at protein (immunohistochemistry and quantitative analysis) and mRNA levels (RT-PCR technique and in situ hybridization histochemistry). Immunohistochemistry for ASIC3 was able to demonstrate dendritic profiles of the periodontal Ruffini endings in the mouse incisors. No thin fibers terminating as nociceptive free nerve endings exhibited ASIC3 immunoreactivity. Double immunofluorescent staining revealed ASIC3 immunoreaction in the axoplasm but not in the ordinary Schwann cells - including the associated terminal Schwann cells. Observation of the trigeminal ganglia showed variously sized neurons expressing ASIC3 immunoreaction; the most intense immunopositivity was found in the small and medium-sized neurons, as confirmed by in situ hybridization histochemistry using a specific cRNA probe. Quantitative analysis on trigeminal ganglion neurons showed that 38.0% of ASIC3 neurons could be categorized as medium-sized neurons which mediate mechanotransduction. These findings suggest that ASIC3 functions as a molecule for mechanosensation in the periodontal Ruffini endings. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

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  • Immunohistochemical detection of ENaC beta in the terminal Schwann cells associated with the periodontal Ruffini endings of the rat incisor 査読

    Yasumasa Hitomi, Akiko Suzuki, Yoshiro Kawano, Kayoko Nozawa-Inoue, Makoto Inoue, Takeyasu Maeda

    BIOMEDICAL RESEARCH-TOKYO   30 ( 2 )   113 - 119   2009年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    Epithelial sodium channels (ENaCs) are a subfamily of ion channels within the degenerin/ENaC (DEG/ENaC) superfamily. Previous studies have shown the immunolocalization of ENaC in the neural elements of the Cutaneous mechanoreceptors as well as dorsal root and trigeminal ganglion neurons, indicating the involvement of this molecule in mechanotransduction. The present study examined the expression of ENaC beta, a major component of ENaC protein, in the mechanoreceptive Ruffini endings in the periodontal ligament of the rat incisors by immunohistochemistry. The expression of ENaC beta in the trigeminal ganglion-which innervates the periodontal Ruffini endings-was also investigated at the mRNA and protein levels. Furthermore, double staining and a nerve injury experiment were applied to clarify its detailed localization in the periodontal Ruffini endings. ENaC beta immunoreaction in the trigeminal ganglion was recognizable in the comparatively large neurons which have been considered to mediate mechanotransduction. Immunohistochemistry for ENaC beta demonstrated dendritic ramifications of the Ruffini endings as well as the rounded cells in the periodontal ligament. Double staining with ENaC beta and either PGP9.5 or S-100 protein showed immunoreaction for ENaC beta in both the axonal and glial elements in the periodontal ligament. Some ENaC beta positive cells with rounded profiles were reactive to non-specific cholinesterase activity. Furthermore, a transection of the inferior alveolar nerve failed to eliminate the rounded cells with ENaC beta reaction, indicating that they were the terminal Schwann cells associated with the periodontal Ruffini endings. These findings suggest that ENaC beta is a key mechanotransducing channel in the periodontal Ruffini endings. Probably, the terminal Schwann cells together with the axon terminals regulate mechanotransduction in the periodontal endings.

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  • Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint 査読

    Akiko Suzuki, Kayoko Nozawa-Inoue, Norio Amizuka, Kazuhiro Ono, Takeyasu Maeba

    Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology   288 ( 6 )   646 - 652   2006年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Previous studies have pointed out a lack of adhesion structures in the synovial lining layer of the rat temporomandibular joint (TMJ) despite showing an epithelial arrangement. CD44, a major cell adhesion molecule, plays crucial roles as an anchor between cells and extracellular matrices by binding hyaluronan (HA) for the development of organs or the metastasis of tumors. The present study examined the localization of CD44 in the synovial membrane of the rat TMJ by immunocytochemistry for OX50, ED1, and Hsp25, which are markers for the rat CD44, macrophage-like type A, and fibroblast-like type B synoviocytes, respectively. Histochemistry for HA-binding protein (HABP) was also employed for the detection of HA. OX50 immunoreactions were found along the cell surface and, in particular, accumulated along the surface of the articular cavity. Observations by a double immunostaining and immunoelectron microscopy revealed that all the OX50-immunopositive cells were categorized as fibroblastic type B cells, which had many caveolae and a few vesicles reactive to intense OX50. However, the macrophage-like type A cells did not have any OX50 immunoreaction in the synovial lining layer. A strong HABP reaction was discernable in the extracellular matrix surrounding both OX50-positive and -negative cells in the synovial lining layers, exhibiting a meshwork distribution, but weak in its sublining layer. This localization pattern of CD44 and HABP might be involved in the formation of the epithelial arrangement of the synovial lining layer. Furthermore, OX50 immunonegativity in the type A cells suggests their low phagocytotic activity in the rat TMJ under normal conditions. © 2006 Wiley-Liss, Inc.

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  • Expression of caveolin-1 in the rat temporomandibular joint 査読

    Nozawa-Inoue Kayoko, Akiko Suzuki, Norio Amizuka, Takeyasu Maeda

    Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology   288 ( 1 )   8 - 12   2006年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This immunocytochemical study revealed the expression of caveolin-1, a major protein of caveolae, in the rat temporomandibular joint. In the synovial lining layer, immunoreactive products for caveolin-1 were detected on the cell membrane of the fibroblast-like type B cells, as confirmed by immunocytochemistry for heat shock protein 25. The cells in the articular disk, the articular layer, and zone of proliferation of the mandibular condyle also showed intense immunoreactions for caveolin-1. © 2005 Wiley-Liss, Inc.

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  • Neurotrophin-4/5-depletion induces a delay in maturation of the periodontal Ruffini endings in mice 査読

    Y Maruyama, F Harada, S Jabbar, Saito, I, M Aita, Y Kawano, A Suzuki, K Nozawa-Inoue, T Maeda

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   68 ( 4 )   267 - 288   2005年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    Neurotrophin-4/5 (NT-4/5)-a member of the neurotrophic factors-is a ligand for TrkB, which has been reported to be expressed in the mechanoreceptive Ruffini endings of the periodontal ligament. The present study examined developmental changes in the terminal morphology and neural density in homozygous mice with a targeted disruption of the nt-4/5 gene and wild-type mice by immunohistochemistry for protein gene product 9.5 (PGP 9.5), a general neuronal marker, and by quantitative analysis using an image analyzer. Postnatal development of terminal Schwann cells was also investigated by enzymatic histochemistry for non-specific cholinesterase activity (ChE). Furthermore, the immuno-expression of TrkB and low affinity nerve growth factor receptor (p75-NGFR) was surveyed in the periodontal Ruffini endings as well as trigeminal ganglion. At postnatal 1 week, the lingual periodontal ligament of both types of mice contained PGP 9.5-positive nerve fibers showing a tree-like ramification with axonal swellings in their course. In both types of mice at 2 weeks of age, comparatively thick nerve fibers with a smooth outline increased in number, and frequently ramified to form nerve terminals with dendritic profiles. However, no typical Ruffini endings with irregular outlines observed in the adult wild-type mice were found in the periodontal ligament at this stage. At postnatal 3 weeks, typical Ruffini endings with irregular outlines were discernable in the periodontal ligament of the wild-type mice while the dendritic endings showing smooth outlines were restricted to the homozygous mice. After postnatal 8 weeks, both types of mice showed an increase in the number of Ruffini endings, but the morphology differed between the wildtype and NT-4/5 homozygous mice. In the wild-type mice, a major population of the Ruffini endings expanded their axonal branches and developed many microprojections, resulting in a reduction of endings with smooth outlines. In contrast, we failed to find such typical Ruffini endings in the periodontal ligament of the homozygous mice: A majority of the periodontal Ruffini endings continued to show smooth outlines at postnatal 12 weeks. Quantitative analysis on neural density demonstrated a reduction in the homozygous mice with a significant difference by postnatal 8 weeks. Enzymatic histochemistry for non-specific ChE did not exhibit a distinct difference in the distribution and density of terminal Schwann cells between wild-type and homozygous mice. Furthermore, TrkB and p75-NGFR mRNA and proteins did not differ in the trigeminal ganglion between the two types. The periodontal Ruffini endings also displayed immunoreactivities for TrkB and p75-NGFR in both phenotypes. These findings suggest that the nt-4/5 gene depletion caused a delay in the formation and maturation of the periodontal Ruffini endings in the mice by inhibiting the growth of the periodontal nerves at an early stage, and indicate that multiple neurotrophins such as NT-4/5 and BDNF might play roles in the development and/or maturation of the periodontal Ruffini endings.

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  • Development of the articular cavity in the rat temporomandibular joint with special reference to the behavior of endothelial cells and macrophages 査読

    Akiko Suzuki, Kayoko Nozawa-Inoue, Nobuyuki Ikeda, Norio Amizuka, Kazuhiro Ono, Ritsuo Takagi, Takeyasu Maeda

    Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology   286 ( 2 )   908 - 916   2005年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Previous developmental studies on the temporomandibular joint (TMJ) have proposed several hypotheses on the formation of its articular cavity. However, detailed information is meager. The present study examined the formation process of the articular cavity in the rat TMJ by immunocytochemistry for CD31, RECA-1, and ED1, which are useful cellular markers for endothelial cells and monocyte/macrophage lineages, respectively. The upper articular cavity formation had begun by embryonic day 21 (E21) and was completed at postnatal day 1 (P1) in advance of the lower cavitation
    the latter took place from P1 to P3. The occurrence and distribution pattern of the CD31-, RECA-1-, and ED1-positive cells differed between the upper and lower articular cavity-forming areas: the ED1-positive cells exclusively occurred in the area of the prospective upper articular cavity prior to its formation, while no ED1-positive cell appeared in the lower cavity-forming area. In contrast, the CD31- and RECA-1-positive endothelial cells were restricted to the lower cavity-forming area (never the prospective upper cavity) at E19 and diminished thereafter. Throughout the cavity formation, we failed to find any apoptotic cells in the cavity formation area, indicating no involvement of apoptosis in the cavity formation in TMJ. The present findings on the behaviors of endothelial cells and ED1-positive cells show a possibility of different mechanism in the cavity formation between the upper and lower articular cavities in the rat TMJ. The appearance of ED1-reactive cells and temporal vascularization may play crucial roles in the upper and lower articular cavity formation, respectively. © 2005 Wiley-Liss, Inc.

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  • Immunocytochemical localization of MAPKAPK-2 and Hsp25 in the rat temporomandibular joint 査読

    Kayoko Nozawa-Inoue, Norio Amizuka, Akiko Suzuki, Takeyasu Maeda

    Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology   284 ( 2 )   522 - 528   2005年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    One series of our research has shown an intense expression of immunoreaction for heat shock protein 25 (Hsp25) in various cellular elements in the rat temporomandibular joint (TMJ). This protein is the major substrate of mitogen-activated protein kinase-activated protein kinase-2 (MAPKAPK-2), which mediates an intracellular stress-activated signaling pathway to stimulate cytosolic actin reorganization under various stresses. The present study was undertaken to examine the localization of MAPKAPK-2 in the rat TMJ by immunocytochemical techniques. Furthermore, confocal microscopy with double staining was employed to demonstrate the colocalization of MAPKAPK-2 and Hsp25. Immunocytochemistry for MAPKAPK-2 showed an intense immunoreaction in the cytoplasm of the synovial lining cells, the endothelial cells, and the fibroblasts in the synovial membrane of the rat TMJ. Double immunostaining under a confocal microscope succeeded in demonstrating the colocalization of MAPKAPK-2 and Hsp25 immunoreactions in the cytoplasm of fibroblastic type B synoviocytes in the TMJ. On the other hand, the macrophage-like type B-cells expressed MAPKAPK-2 immunoreactions but lacked Hsp25 immunoreactivity. The cells in the articular disk and the chondrocytes in the maturative and hypertrophic layer of the mandibular cartilage also showed intense immunoreactions for MAPKAPK-2 and Hsp25. In addition to cytoplasmic localization, MAPKAPK-2 immunoreactions were found in the nucleus of some synovial lining cells, cells in the articular disk, and chondrocytes. Current observations imply the presence of the phosphorylation of Hsp25 via activated MAPKAPK-2 in the cytoplasm. MAPKAPK-2 and Hsp25 possibly participate in the induction of cytoskeletal changes to the various cellular elements in rat TMJ under normal conditions. © 2005 Wiley-Liss, Inc.

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  • Reduced osteoblastic population and defective mineralization in osteopetrotic (op/op) mice 査読

    N Sakagami, N Amizuka, MQ Li, K Takeuchi, M Hoshino, M Nakamura, K Nozawa-Inoue, N Udagawa, T Maeda

    MICRON   36 ( 7-8 )   688 - 695   2005年

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Osteopetrotic (op/op) mice fail to exhibit bone remodeling because of a defective osteoclast formation due to a lack of macrophage colony-stimulating factor. In this study, we investigated the femora of op/op mice to clarify whether the osteoblastic population and bone mineralization are involved in osteoclasts or their bone resorption. The op/op mice extended the meshwork of trabecular bones from the chondro-osseous junction to the diaphyseal region. In the femoral metaphyses of op/op mice, intense alkaline phosphatase (ALPase)-positive osteoblasts were observed on the metaphyseal bone in close proximity to the erosion zone of the growth plates. Von Kossa's staining revealed scattered mineralized nodules and a fine meshwork of mineralized bone matrices while the wild-type littermates developed well-mineralized trabeculae parallel to the longitudinal axis. In contrast to the metaphysis, some op/op diaphyses showed flattened osteoblasts with weak ALPase-positivity, and the other diaphyses displayed bone surfaces without a covering by osteoblasts. It is likely, therefore, that the osteoblastic population and activity were lessened in the op/op diaphyses. Despite the osteopetrotic model, von Kossa's staining demonstrated patchy unmineralized areas in the op/op diaphyses, indicating that a lower population and/or the activity of osteoblasts resulted in defective mineralization in the bone. Transmission electron microscopy disclosed few osteoblasts on the diaphyseal bones, and instead, bone marrow cells and vascular endothelial cells were often attached to the unmineralized bone. Osteocytes were embedded in the unmineralized bone matrix. Thus, osteoclasts appear to be involved in the osteoblastic population and activity as well as subsequent bone mineralization. (c) 2005 Elsevier Ltd. All rights reserved.

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  • Expression of estrogen receptor alpha (ER alpha) in the rat temporomandibular joint. 査読

    Yamada K, Nozawa-Inoue K, Kawano Y, Kohno S, Amizuka N, Iwanaga T, Maeda T

    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology   274 ( 2 )   934 - 941   2003年10月

  • Temporal expression of immunoreactivity for heat shock protein 25 (Hsp25) in the rat periodontal ligament following transection of the inferior alveolar nerve 査読

    K Iijima, F Harada, K Hanada, K Nozawa-Inoue, M Aita, Y Atsumi, S Wakisaka, T Maeda

    BRAIN RESEARCH   979 ( 1-2 )   146 - 152   2003年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The present study examined the immunohistochemical localization of heat shock protein 25 (Hsp25) during the regeneration of nerve fibers and Schwann cells in the periodontal ligament of the rat lower incisor following transection of the inferior alveolar nerve. In the untreated control group, the periodontal ligament of rat incisor did not contain any Hsp25-immunoreaction. On postoperative day 3 (PO 3d), a small number of Schwann cells with slender cytoplasmic processes exhibited Hsp25-immunoreactivity. From PO 5d to PO 21d, Hsp25-positive nerve fibers and Schwann cells drastically increased in number in the alveolar half of the ligament. Although the axons of some regenerating Ruffini-like endings also showed Hsp25-immunoreactions, the migrated Schwann cells were devoid of Hsp25-immunoreaction. Thereafter, Hsp25-positive structures decreased in number gradually to disappear from the periodontal ligament by PO 56d. This temporal expression of Hsp25 in the periodontal ligament well-reflected the regeneration process of the nerve fibers. Hsp25 in the regenerating nerve fibers and denervated Schwann cells most likely serves in modulating actin dynamics and as a cellular inhibitor of apoptosis, respectively. (C) 2003 Elsevier B.V. All rights reserved.

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  • Convergence of selected inputs premotor neurons with possible from sensory afferents to trigeminal projections to masseter motoneurons in the rabbit 査読

    M Inoue, K Nozawa-Inoue, R Donga, Y Yamada

    BRAIN RESEARCH   957 ( 1 )   183 - 191   2002年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Peripheral input convergence on trigeminal premotor neurons in the vicinity of trigeminal motor nucleus has been investigated. Thirty neurons were identified by their antidromic responses to microstimulation of the masseteric subnucleus of trigeminal motor nucleus (NVmot-mass). Peripheral receptive fields were found in the buccal mucosae, periodontal ligaments, palate, tongue and vibrissae for 16 neurons located in the intertrigeminal area (NVint), supratrigeminal area (NVs), main sensory trigeminal nucleus (NVsnpr) and subnucleus gamma of the oral nucleus of the spinal trigeminal tract (NVspo-gamma). Eleven neurons in the NVint, NVs and NVspo-gamma responded to passive jaw opening: nine neurons were activated and two were inhibited. None of the neurons responded to both the orofacial mechanical stimulation and passive jaw opening. Forty-six percent of neurons (13 out of 28 tested) received inputs from the inferior alveolar nerve (IAN) and 53% of neurons (8 out of 15 tested) received inputs from the infraorbital nerve (ION). Out of 15 neurons tested for inputs from the IAN and ION, 7 neurons in the NVsnpr and NVspo-gamma received input from both. Sixteen percent of neurons (4 out of 25) received inputs from the masseteric nerve (MassN). None of the neurons with inputs from IAN and/or ION also received inputs from the MassN. We suggest that trigeminal premotor interneurons with projections to the NVmot-mass fall into two broad categories, those with inputs from the IAN and/or ION and those with inputs from the MassN, possibly muscle spindle afferents, and no neuron receiving inputs from both. (C) 2002 Elsevier Science B.V. All rights reserved.

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  • Expression of 25 kDa heat shock protein by synovial type B cells of the mouse temporomandibular joint 査読

    E Andoh, Y Kawano, H Ajima, K Nozawa-Inoue, S Kohno, T Maeda

    ARCHIVES OF ORAL BIOLOGY   46 ( 10 )   947 - 954   2001年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Earlier studies have demonstrated immunoreactivity for heat shock protein 25 (Hsp25) in type B synovial lining cells of the rat temporomandibular joint, and also the presence of characteristic cytoplasmic processes in these cells, but it is unclear whether or not the type B cells in other animals possess such elaborate cytoplasmic projections and as there is as yet no evidence for the synthesis of this protein by these cells. For these reasons, the expression of Hsp25 was investigated in the synovial membrane of the mouse temporomandibular joint by immunocytochemistry and by in situ hybridization using a specific cRNA probe, Intense immunoreaction for Hsp25 was found in the cytoplasm of certain synovial lining cells that were identified as type B by immunoelectron-microscopy. These Hsp25-positive cells had slender cytoplast-nic processes, either projecting towards or covering the synovial surface. Morphological differences between cytoplasmic processes seemed to depend on the location of the type B cell bodies. In situ hybridization showed intense signals for Hsp25 mRNA in the synovial lining cells, suggesting that the type B cells produce, rather than resorb, Hsp25. These findings indicate that Hsp25 is a useful marker for the identification of the synovial type B cells in the temporomandibular joint. It is further hypothesized that Hsp25 in type B cells is involved in maintaining their specific profile and epithelial-like arrangement, and in protecting against mechanical stress. (C) 2001 Elsevier Science Ltd. All rights reserved.

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  • Changes in jaw reflexes by stimulation of the hypothalamus in anesthetized rabbits 査読

    M Inoue, K Nozawa-Inoue, Y Miyaoka, Y Yamada

    NEUROSCIENCE RESEARCH   41 ( 1 )   61 - 65   2001年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    Changes in the masseteric monosynaptic reflex (MMR) and jaw-opening reflex (JOR) responses resulting from conditioning stimulation in the hypothalamus were studied in anesthetized rabbits. Stimulation of the lateral hypothalamus evoked a facilitation of the MMR and an inhibitory or facilitatory effect on the JOR. The facilitatory effect on JOR was stronger than that on the MMR. The facilitatory effective site for the JOR was in the dorsal and lateral directions as compared to the inhibitory field. The results suggest two functionally distinct regions in the lateral hypothalamus that separately project to the jaw-opening muscles. (C) 2001 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

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  • Immunocytochemical detection of superoxide dismutases (SODs) in the periodontal Ruffini endings of the rat incisor 査読

    H Yamamoto, S Hayashi, K Nakakura-Ohshima, Y Kawano, K Nozawa-Inoue, H Ohshima, T Maeda

    BRAIN RESEARCH   905 ( 1-2 )   232 - 235   2001年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The expression of immunoreactivities for superoxide dismutases (SODs), Mn-SOD and Cu/Zn-SOD. was immunohistochemically investigated in the lingual periodontal ligament and toe pads of adult rats. Immunocytochemistry for SODs revealed that the axon terminals of both the periodontal Ruffini endings and cutaneous Meissner's corpuscles showed mitochondrial Mn-SOD immunoreactivity, but not cytosolic Cu/Zn-SOD immunoreactivity, indicating Mn-SOD is a useful marker for identifying the mechanoreceptors. It is likely that Mn-SOD in the axon terminals of mechanoreceptors exerts protective action against nerve injury and neuronal death under severe conditions, serving to scavenge free radicals from the axon terminals. (C) 2001 Elsevier Science B.V. All rights reserved.

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  • Transient expression of heat shock protein (Hsp) 25 in the dental pulp and enamel organ during odontogenesis in the rat incisor 査読

    H Ohshima, H Ajima, Y Kawano, K Nozawa-Inoue, S Wakisaka, T Maeda

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   63 ( 4 )   381 - 395   2000年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    The expression of heat shock protein (Hsp) 25 during odontogenesis in the dental pulp and enamel organ of rat incisors was investigated by immunocytochemistry and confocal microscopy. In the process of dentin formation, immature odontoblasts first exhibited Hsp 25-immunoreactivity, and increased in immunointensity with the advance of their differentiation. In the dental pulp, in contrast, intense immunoreaction in the mesenchymal cells became weak or negative in parallel with the progress of cell differentiation. The immunoreaction for Hsp 25 in the enamel organ revealed a characteristic stage-related alteration during amelogenesis. In secretory ameloblasts, the immunoreaction for Hsp 25 was found throughout their cell bodies, intense reactivity being located near the proximal and distal terminal webs. At the maturation stage, ruffle-ended ameloblasts (RA) consistently showed Hsp 25-immunoreactivity throughout the cell bodies, whereas smooth-ended ameloblasts (SA) lacking a ruffled border were weak in immunoreaction at the distal cytoplasm. Other cellular elements of the enamel organ were negative. The subcellular localization of Hsp 25-immunoreactivity in this study appeared essentially identical to that of actin filaments as demonstrated by confocal microscopy using rhodamine-labeled phalloidin. These immunocytochemical data suggest that the Hsp 25 molecule is involved in reinforcement of the cell layer following cell movement during odontogenesis and in the formation and maintenance of the ruffled border of RA.

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  • Morphology and functional roles of synoviocytes in the joint 査読

    T Iwanaga, M Shikichi, H Kitamura, H Yanase, K Nozawa-Inoue

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   63 ( 1 )   17 - 31   2000年3月

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    記述言語:英語   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    The joint capsule exhibits a unique cellular lining in the luminal surface of the synovial membrane. The synovial intimal cells, termed synoviocytes, are believed to be responsible for the production of synovial fluid components, for absorption from the joint cavity, and for blood/synovial fluid exchanges, but their detailed structure and function as well as pathological changes remain unclear. Two types of synoviocytes, macrophagic cells (type A cells) and fibroblast-like cells (type B cells) have been identified. Type A synoviocytes are non-fixed cells that can phagocytose actively cell debris and wastes in the joint cavity, and possess an antigen-presenting ability. These type a cells, derived from blood-borne mononuclear cells, can be considered resident macrophages (tissue macrophages) like hepatic Kupffer cells. Type B synoviocytes are characterized by the rich existence of rough endoplasmic reticulum, and dendritic processes which form a regular network in the luminal surface of the synovial membrane. Their complex three-dimensional architecture was first revealed by our recent scanning electron microscopy of macerated samples. The type B cells, which are proper synoviocytes, are involved in production of specialized matrix constituents including hyaluronan, collagens and fibronectin for the intimal interstitium and synovial fluid. The proliferative potentials of type B cells in loco are much higher than type B cells, although the transformation of subintimal fibroblasts into type B cells can not be excluded. In some mammals, type B cells show features suggesting endocrine and sensory functions, but these are not recognized in other species. The synoviocytes, which form a discontinuous cell layer, develop both fragmented basement membranes around the cells and junctional apparatus such as desmosomes and gap junctions. For an exact understanding of the mechanism of arthritis, we need to establish the morphological background of synoviocytes as well as their functions under normal conditions.

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  • Immunocytochemical demonstration of heat shock protein 25 in the rat temporomandibular joint 査読

    K Nozawa-Inoue, H Ohshima, Y Kawano, H Yamamoto, R Takagi, T Maeda

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   62 ( 5 )   483 - 491   1999年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    The expression of heat shock protein 25 (Hsp 25) was investigated in the rat temporomandibular joint by immunocytochemistry combined with confocal and electron microscopy. Immunostaining with an antibody to Hsp25 was able to demonstrate various cellular elements in the synovial membrane of the joint. Intense immunoreaction for Hsp25 was recognized in certain cells comprising the synovial lining layer. Confocal microscopic observation revealed two characteristic profiles of the Hsp25-positive cells with cytoplasmic processes: one extended thick and long processes towards the articular cavity, and the other prejected horizontally slender processes which covered the synovial membrane. Under the electron microscope, the immunoreactive synovial lining cells were characterized by a well-developed rough endoplasmic reticulum and secretory granules, suggesting that they can be categorized as fibroblastic type B cells. The covering by the cytoplasmic extensions was confirmed by immune-electron microscopic observations. This cytoplasmic covering presumably performs a barrier function and expedites the effective secretion/resorption of synovial fluids. Since it has been proposed that Hsp 25 is associated with an estrogen receptor, the immunopositive synovial lining cells were considered estrogen-target cells. Immunoreactivity for Hsp25 was also observed in the chondrocytes of the maturative and hypertrophic cell layers as well as in the cells of the articular disk. A suggestion was made that Hsp 25 might be involved in the inhibition of apoptosis of those cells.

    DOI: 10.1679/aohc.62.483

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  • Immunocytochemical demonstration of laminin in the synovial lining layer of the rat temporomandibular joint 査読

    K Nozawa-Inoue, H Ajima, R Takagi, T Maeda

    ARCHIVES OF ORAL BIOLOGY   44 ( 6 )   531 - 534   1999年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    This immunocytochemical study describes the distribution of laminin in the synovial lining of the rat temporomandibular joint. Laminin immunostaining was present around some synovial lining cells and blood vessels. Ultrastructurally, immunoreactive products for laminin were deposited around cells with a well-developed rough endoplasmic reticulum and secretory granules, suggesting that they were type B synovial lining cells. The localization of laminin immunoreactivity was not uniform around the cell membrane, the most intense immunoreaction being present on the basal aspect membrane as is seen in the basement membrane of epithelia. In contrast, macrophage-like synovial lining type A cells did not show laminin immunoreactivity. This different immunostaining pattern suggests that laminin acts as an adhesion molecule for the type B cells in their epitheliallike arrangement. (C) 1999 Elsevier Science Ltd. All rights reserved.

    DOI: 10.1016/S0003-9969(99)00021-7

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書籍等出版物

  • 井上佳世子:I編 人体の構造と機能 1章 解剖学.歯科衛生士 書き込み式学習ノート①専門基礎科目編,

    井上 佳世子( 範囲: 4-42頁)

    医歯薬出版  2018年 

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  • 井上佳世子:9章 顎関節.ネッター 頭頸部・口腔顎顔面の臨床解剖学アトラス(原著第3版改訂)(前田健康監訳)

    井上 佳世子( 担当: 共訳 ,  範囲: 239-253頁)

    医歯薬出版  2018年 

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  • 野澤-井上佳世子,前田健康:第8章 III.顎関節の発生.口腔組織・発生学 第2版(脇田 稔,前田健康,中村浩彰,網塚憲生編)

    井上 佳世子( 担当: 共著 ,  範囲: 246-252頁)

    医歯薬出版  2018年 

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  • 顎関節の発生.口腔組織・発生学 第2版(脇田 稔,前田健康,中村浩彰,網塚憲生編)

    井上 佳世子( 範囲: 246-252頁)

    医歯薬出版  2015年 

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  • 顎関節.最新歯科衛生士教本 歯・口腔の構造と機能 口腔解剖学・口腔組織発生学・口腔生理学(前田健康,遠藤圭子,畠中能子編)

    井上 佳世子( 範囲: 42-45頁)

    医歯薬出版  2011年 

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  • 消化器の構造.最新歯科衛生士教本 人体の構造と機能1 解剖学・組織発生学・生理学(前田健康,山田小枝子編)

    井上 佳世子( 担当: 共著 ,  範囲: 87-98頁)

    医歯薬出版  2010年 

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  • 細胞と組織.最新歯科衛生士教本 人体の構造と機能1 解剖学・組織発生学・生理学(前田健康,山田小枝子編)

    井上 佳世子( 担当: 共著 ,  範囲: 14-31頁)

    医歯薬出版  2010年 

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    担当ページ:14-31頁   著書種別:教科書・概説・概論

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  • 野澤-井上佳世子,前田健康:顎関節の構造(下野正基,前田健康,溝口 到編).歯の移動の臨床バイオメカニクス-骨と歯根膜のダイナミズム-,173-187頁

    医歯薬出版  2006年 

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  • 野澤-井上佳世子,前田健康:顎関節の発生(脇田 稔,明坂年隆,前田健康,山下靖雄編).口腔組織・発生学,294-300頁

    医歯薬出版  2006年 

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MISC

  • 3D口腔粘膜モデルを用いたビスフォスフォネート製剤が創閉鎖に及ぼす影響の組織学的・免疫組織化学的検討

    齋藤 太郎, 泉 健次, 上野山 敦士, 塩見 晶, 大貫 尚志, 加藤 寛子, 寺田 典子, 河野 芳朗, 野澤 佳世子, 高木 律男, 前田 健康

    新潟歯学会雑誌   42 ( 2 )   142 - 143   2012年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • Histological and immunohistological analyses of epithelial regeneration and wound healing affected by Bisphosphonates (BPs) using a living oral mucosa equivalent model

    T. Saito, K. Izumi, A. Shiomi, H. Ohnuki, H. Kato, M. Terada, Y. Kawano, K. Nozawa-Inoue, R. Takagi, T. Maeda

    JOURNAL OF INVESTIGATIVE DERMATOLOGY   132   S135 - S135   2012年5月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:NATURE PUBLISHING GROUP  

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  • ゾレドロン酸がヒト口腔粘膜上皮細胞に及ぼす影響に関する研究

    大貫 尚志, 泉 健次, 加藤 寛子, 齋藤 太郎, 寺田 典子, 河野 芳郎, 野澤 佳世子, 高木 律男, 前田 健康

    新潟歯学会雑誌   41 ( 2 )   119 - 120   2011年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • アクアポリン-1を指標にしたラット歯根膜硝子様変性組織の出現・消失過程

    吉居 朋子, 原田 史子, 河野 芳朗, 野澤 佳世子, 齋藤 功, 前田 健康

    新潟歯学会雑誌   41 ( 2 )   127 - 128   2011年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • 実験的矯正移動にともなうアクアポリン1陽性細胞の観察

    Binte Anwar Humayra, 河野 芳朗, 木下 承子, 野澤 佳世子, 齋藤 功, 前田 健康

    Journal of Oral Biosciences   53 ( Suppl. )   151 - 151   2011年9月

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    記述言語:英語   出版者・発行元:(一社)歯科基礎医学会  

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  • 歯科インプラント周囲における骨細胞・骨細管系の組織化学的変化

    羽下 麻衣子, 野澤 佳世子, 上, 李 敏啓, 吉江 紀夫, 網塚 憲生, 前田 健康

    Journal of Oral Biosciences   52 ( Suppl )   128 - 128   2010年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • GDNF Expression in Terminal Schwann Cells Associated With the Periodontal Ruffini Endings of the Rat Incisors During Nerve Regeneration

    Megumi Ohishi, Fumiko Harada, Farhana Rahman, Isao Saito, Yoshiro Kawano, Kayoko Nozawa-Inoue, Takeyasu Maeda

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY   292 ( 8 )   1182 - 1191   2009年8月

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    記述言語:英語   出版者・発行元:WILEY  

    The terminal Schwann cells (TSCs) which play crucial roles in regeneration of the periodontal Ruffini endings (RE) exhibit immunoreaction for glial cell line-derived neurotrophic factor (GDNF). However, no information is available regarding the role of GDNF in the periodontal RE during nerve regeneration. This study was undertaken to examine the changes in GDNF expression in the rat periodontal RE following transection of the inferior alveolar nerve (IAN) using immunohistochemistry for GDNF and S-100 protein, a marker for the TSCs. We additionally investigated the changes in expression of GDNF in the trigeminal ganglion (TG) at protein and mRNA levels. A transection to IAN induced a disappearance of the TSCs from the alveolus-related part (ARP), followed by a migration of spindle-shaped cells with S-100 but without GDNF immunoreactions into the tooth-related part (TRP) by postoperative (PO) week 2. At PO week 2, GDNF immunoreacted cellular elements increased in number in the ARP although the spindle-shaped cells without GDNF reaction remained in the TRP After PO week 4, many GDNF-positive TSCs appeared in the ARP though the spindle-shaped cells vanished from the TRP. A real time RT-PCR analysis demonstrated the highest elevation of GDNT mRNA in the TG at PO week 2. These findings suggested the involvement of this molecule in the maturation and maintenance of the periodontal RE during regeneration. Taken together with our previous and current studies, it appears that the regeneration of the periodontal RE is controlled by multiple neurotrophins in a stage-specific manner. Anat Rec, 292:1182-1191, 2009. (C) 2009 Wiley-Liss, Inc.

    DOI: 10.1002/ar.20931

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  • GDNF Expression in Terminal Schwann Cells Associated With the Periodontal Ruffini Endings of the Rat Incisors During Nerve Regeneration

    Megumi Ohishi, Fumiko Harada, Farhana Rahman, Isao Saito, Yoshiro Kawano, Kayoko Nozawa-Inoue, Takeyasu Maeda

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY   292 ( 8 )   1182 - 1191   2009年8月

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    記述言語:英語   出版者・発行元:WILEY  

    The terminal Schwann cells (TSCs) which play crucial roles in regeneration of the periodontal Ruffini endings (RE) exhibit immunoreaction for glial cell line-derived neurotrophic factor (GDNF). However, no information is available regarding the role of GDNF in the periodontal RE during nerve regeneration. This study was undertaken to examine the changes in GDNF expression in the rat periodontal RE following transection of the inferior alveolar nerve (IAN) using immunohistochemistry for GDNF and S-100 protein, a marker for the TSCs. We additionally investigated the changes in expression of GDNF in the trigeminal ganglion (TG) at protein and mRNA levels. A transection to IAN induced a disappearance of the TSCs from the alveolus-related part (ARP), followed by a migration of spindle-shaped cells with S-100 but without GDNF immunoreactions into the tooth-related part (TRP) by postoperative (PO) week 2. At PO week 2, GDNF immunoreacted cellular elements increased in number in the ARP although the spindle-shaped cells without GDNF reaction remained in the TRP After PO week 4, many GDNF-positive TSCs appeared in the ARP though the spindle-shaped cells vanished from the TRP. A real time RT-PCR analysis demonstrated the highest elevation of GDNT mRNA in the TG at PO week 2. These findings suggested the involvement of this molecule in the maturation and maintenance of the periodontal RE during regeneration. Taken together with our previous and current studies, it appears that the regeneration of the periodontal RE is controlled by multiple neurotrophins in a stage-specific manner. Anat Rec, 292:1182-1191, 2009. (C) 2009 Wiley-Liss, Inc.

    DOI: 10.1002/ar.20931

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  • Immunohistochemical detection of ENaC beta in the terminal Schwann cells associated with the periodontal Ruffini endings of the rat incisor

    Yasumasa Hitomi, Akiko Suzuki, Yoshiro Kawano, Kayoko Nozawa-Inoue, Makoto Inoue, Takeyasu Maeda

    BIOMEDICAL RESEARCH-TOKYO   30 ( 2 )   113 - 119   2009年4月

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    記述言語:英語   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    Epithelial sodium channels (ENaCs) are a subfamily of ion channels within the degenerin/ENaC (DEG/ENaC) superfamily. Previous studies have shown the immunolocalization of ENaC in the neural elements of the Cutaneous mechanoreceptors as well as dorsal root and trigeminal ganglion neurons, indicating the involvement of this molecule in mechanotransduction. The present study examined the expression of ENaC beta, a major component of ENaC protein, in the mechanoreceptive Ruffini endings in the periodontal ligament of the rat incisors by immunohistochemistry. The expression of ENaC beta in the trigeminal ganglion-which innervates the periodontal Ruffini endings-was also investigated at the mRNA and protein levels. Furthermore, double staining and a nerve injury experiment were applied to clarify its detailed localization in the periodontal Ruffini endings. ENaC beta immunoreaction in the trigeminal ganglion was recognizable in the comparatively large neurons which have been considered to mediate mechanotransduction. Immunohistochemistry for ENaC beta demonstrated dendritic ramifications of the Ruffini endings as well as the rounded cells in the periodontal ligament. Double staining with ENaC beta and either PGP9.5 or S-100 protein showed immunoreaction for ENaC beta in both the axonal and glial elements in the periodontal ligament. Some ENaC beta positive cells with rounded profiles were reactive to non-specific cholinesterase activity. Furthermore, a transection of the inferior alveolar nerve failed to eliminate the rounded cells with ENaC beta reaction, indicating that they were the terminal Schwann cells associated with the periodontal Ruffini endings. These findings suggest that ENaC beta is a key mechanotransducing channel in the periodontal Ruffini endings. Probably, the terminal Schwann cells together with the axon terminals regulate mechanotransduction in the periodontal endings.

    DOI: 10.2220/biomedres.30.113

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  • Immunohistochemical detection on ENaCβ in the terminal Schwann cells associated with the periodontal Ruffini endings of the rat incisor.

    Hitomi Y, Suzuki A, Kawano Y, Nozawa-Inoue K, Inoue M, Maeda T

    Biomed. Res.   30 ( 2 )   113 - 119   2009年4月

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  • Differential cell-specific location of Cav-1 and Ca2+-ATPase in terminal Schwann cells and mechanoreceptive Ruffini endings in the periodontal ligament of the rat incisor

    Naoyuki Iizuka, Akiko Suzuki, Kayoko Nozawa-Inoue, Yoshiro Kawano, B. G. T. L. Nandasena, Takashi Okiji, Takeyasu Maeda

    JOURNAL OF ANATOMY   214 ( 2 )   267 - 274   2009年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Caveolae are involved in clathrin-independent endocytosis, transcytosis, signal transduction, and tumor suppression - all of which depend on their main constituent protein caveolin families. The periodontal Ruffini ending has been reported to develop a caveola-like structure on the cell membrane of both the axon terminals and Schwann sheaths, suggesting the existence of an axon-Schwann cell interaction in the periodontal Ruffini endings. However, little information is available concerning the functional significance of these caveolae. The present study was undertaken to examine the immunolocalization of caveolin-1, -3 (Cav-1, Cav-3) and Ca2+-ATPase in the periodontal Ruffini endings of the rat incisor. Decalcified sections of the upper jaws were processed for immunocytochemistry at the levels of light and electron microscopy. Some immunostained sections were treated with histochemistry for nonspecific cholinesterase (nChE) activity. Observations showed the periodontal Ruffini endings were immunopositive for Cav-1, but not Cav-3. Immunoreactive products for Cav-1 were confined to caveola-like structures in the cell membranes of the cytoplasmic extensions and cell bodies of the terminal Schwann cells associated with the periodontal Ruffini endings. However, the axonal membranes of the terminals did not express any Cav-1 immunoreaction. Double staining with Ca2+-ATPase and either protein gene product 9.5 (PGP 9.5) or S-100 protein disclosed the co-localization of immunoreactions in the axonal branches of the periodontal Ruffini endings, but not in the terminal Schwann cells. As Ca2+ plays an important role in mechanotransduction, these characteristic immunolocalizations show Cav-1/Ca2+-ATPase might be involved in the quick elimination of intracellular Ca2+ in mechanotransduction.

    DOI: 10.1111/j.1469-7580.2008.01029.x

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  • Differential cell-specific location of Cav-1 and Ca2+-ATPase in terminal Schwann cells and mechanoreceptive Ruffini endings in the periodontal ligament of the rat incisor

    Naoyuki Iizuka, Akiko Suzuki, Kayoko Nozawa-Inoue, Yoshiro Kawano, B. G. T. L. Nandasena, Takashi Okiji, Takeyasu Maeda

    JOURNAL OF ANATOMY   214 ( 2 )   267 - 274   2009年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Caveolae are involved in clathrin-independent endocytosis, transcytosis, signal transduction, and tumor suppression - all of which depend on their main constituent protein caveolin families. The periodontal Ruffini ending has been reported to develop a caveola-like structure on the cell membrane of both the axon terminals and Schwann sheaths, suggesting the existence of an axon-Schwann cell interaction in the periodontal Ruffini endings. However, little information is available concerning the functional significance of these caveolae. The present study was undertaken to examine the immunolocalization of caveolin-1, -3 (Cav-1, Cav-3) and Ca2+-ATPase in the periodontal Ruffini endings of the rat incisor. Decalcified sections of the upper jaws were processed for immunocytochemistry at the levels of light and electron microscopy. Some immunostained sections were treated with histochemistry for nonspecific cholinesterase (nChE) activity. Observations showed the periodontal Ruffini endings were immunopositive for Cav-1, but not Cav-3. Immunoreactive products for Cav-1 were confined to caveola-like structures in the cell membranes of the cytoplasmic extensions and cell bodies of the terminal Schwann cells associated with the periodontal Ruffini endings. However, the axonal membranes of the terminals did not express any Cav-1 immunoreaction. Double staining with Ca2+-ATPase and either protein gene product 9.5 (PGP 9.5) or S-100 protein disclosed the co-localization of immunoreactions in the axonal branches of the periodontal Ruffini endings, but not in the terminal Schwann cells. As Ca2+ plays an important role in mechanotransduction, these characteristic immunolocalizations show Cav-1/Ca2+-ATPase might be involved in the quick elimination of intracellular Ca2+ in mechanotransduction.

    DOI: 10.1111/j.1469-7580.2008.01029.x

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  • Detailed Process of Bone Remodeling After Achievement of Osseointegration in a Rat Implantation Model

    Maiko Haga, Noritaka Fujii, Kayoko Nozawa-Inoue, Shuichi Nomura, Kimimitsu Oda, Katsumi Uoshima, Takeyasu Maeda

    Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology   292 ( 1 )   38 - 47   2009年1月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Osseointegration is regarded as the most appropriate implant-bone interface in dental implantation. However, damaged bone with empty osteocytic lacunae driven by implant cavity preparation remains even after the completion of osseointegration. Although previous studies have suggested the occurrence of bone remodeling around implants, information on its detailed process is meager. Our study aimed to examine the fate of bone around titanium implants after the establishment of osseointegration on an animal model using the rat maxilla. Titanium implants were inserted into prepared bone cavities of the rat maxilla. Bone formation and maturation processes were evaluated by double staining for alkaline phosphatase and tartrate-resistant acid phosphatase, immunohistochemistry for bone matrix proteins, vital staining with calcein, and elemental mapping with an electron probe microanalyzer. Bone with empty osteocytic lacunae or pyknosis remained between the intact preexisting and newly formed woven bones at post 1 month. It gradually decreased to disappear completely by active bone remodeling with a synchronized coordination of alkaline phosphatase-positive osteoblasts and tartrate-resistant acid phosphatase-reactive osteoclasts at post 3 months, thickening to be replaced by compact bone. Dynamic labeling showed two clear lines in the newly formed bone around the implant through this experimental period. Electron probe microanalyzer analysis demonstrated chronologically increased levels of Ca and P in the newly formed bone identical to those in the surrounding bone at post 2.5 months. These findings indicate that continuous bone remodeling after the achievement of osseointegration causes replacement of the damaged bone by compact bone as well as an improvement in bone quality. Anat Rec,292:38-47, 2009. (c) 2008 Wiley-Liss, Inc.

    DOI: 10.1002/ar.20748

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  • Immunohistochemical detection of nestin in the periodontal Ruffini endings of the rat incisor

    Shion Saito, Akiko Suzuki, Kayoko Nozawa-Inoue, Yoshiro Kawano, Masaaki Hoshino, Chikara Saito, Takeyasu Maeda

    NEUROSCIENCE LETTERS   449 ( 3 )   195 - 200   2009年1月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    Nestin is an intermediate filament which was first identified in neuroepithelial stem cells. This expression has also been reported in restricted locations in adults. Previous studies have suggested that the periodontal Ruffini endings remain immature in nature even in adulthood. The present study reports on a characteristic expression of immunoreaction for nestin in the periodontal Ruffini endings during postnatal development. RT-PCR analysis detected nestin mRNA in a reverse transcripted cDNA sample from both the rat trigeminal ganglion and periodontal ligament. The nestin immunoreaction existed in the periodontal ligament at postnatal day 3 (PO 3 days), when many spindle-shaped Schwann cells were positive for nestin immunoreaction. At PO 1 week, when periodontal nerve fibers displayed a dendritic fashion, the round cells came to show the nestin immunoreaction. These immunopositive cells were also reactive for S-100 protein and non-specific cholinesterase, indicating that these cells could be categorized as terminal Schwann cells associated with the periodontal Ruffini endings. Some ordinary Schwann cells also exhibited nestin immunoreaction. From PO 2 to 3 weeks, nestin positive terminal Schwarm cells increased in number in accordance with the postnatal development of the periodontal Ruffini endings, while this immuno-expression pattern remained unchanged. Nestin immunoreaction was also recognizable in the satellite cells - but never in the neurons - in the trigeminat ganglion throughout this observation period. This immuno-expression pattern suggests that nestin serves as an intermediate filament for mechanical stability in the periodontal Ruffini endings against external stimuli. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2008.11.001

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  • Immunohistochemical detection of nestin in the periodontal Ruffini endings of the rat incisor

    Shion Saito, Akiko Suzuki, Kayoko Nozawa-Inoue, Yoshiro Kawano, Masaaki Hoshino, Chikara Saito, Takeyasu Maeda

    NEUROSCIENCE LETTERS   449 ( 3 )   195 - 200   2009年1月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    Nestin is an intermediate filament which was first identified in neuroepithelial stem cells. This expression has also been reported in restricted locations in adults. Previous studies have suggested that the periodontal Ruffini endings remain immature in nature even in adulthood. The present study reports on a characteristic expression of immunoreaction for nestin in the periodontal Ruffini endings during postnatal development. RT-PCR analysis detected nestin mRNA in a reverse transcripted cDNA sample from both the rat trigeminal ganglion and periodontal ligament. The nestin immunoreaction existed in the periodontal ligament at postnatal day 3 (PO 3 days), when many spindle-shaped Schwann cells were positive for nestin immunoreaction. At PO 1 week, when periodontal nerve fibers displayed a dendritic fashion, the round cells came to show the nestin immunoreaction. These immunopositive cells were also reactive for S-100 protein and non-specific cholinesterase, indicating that these cells could be categorized as terminal Schwann cells associated with the periodontal Ruffini endings. Some ordinary Schwann cells also exhibited nestin immunoreaction. From PO 2 to 3 weeks, nestin positive terminal Schwarm cells increased in number in accordance with the postnatal development of the periodontal Ruffini endings, while this immuno-expression pattern remained unchanged. Nestin immunoreaction was also recognizable in the satellite cells - but never in the neurons - in the trigeminat ganglion throughout this observation period. This immuno-expression pattern suggests that nestin serves as an intermediate filament for mechanical stability in the periodontal Ruffini endings against external stimuli. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2008.11.001

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  • Detailed Process of Bone Remodeling After Achievement of Osseointegration in a Rat Implantation Model

    Maiko Haga, Noritaka Fujii, Kayoko Nozawa-Inoue, Shuichi Nomura, Kimimitsu Oda, Katsumi Uoshima, Takeyasu Maeda

    Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology   292 ( 1 )   38 - 47   2009年1月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Osseointegration is regarded as the most appropriate implant-bone interface in dental implantation. However, damaged bone with empty osteocytic lacunae driven by implant cavity preparation remains even after the completion of osseointegration. Although previous studies have suggested the occurrence of bone remodeling around implants, information on its detailed process is meager. Our study aimed to examine the fate of bone around titanium implants after the establishment of osseointegration on an animal model using the rat maxilla. Titanium implants were inserted into prepared bone cavities of the rat maxilla. Bone formation and maturation processes were evaluated by double staining for alkaline phosphatase and tartrate-resistant acid phosphatase, immunohistochemistry for bone matrix proteins, vital staining with calcein, and elemental mapping with an electron probe microanalyzer. Bone with empty osteocytic lacunae or pyknosis remained between the intact preexisting and newly formed woven bones at post 1 month. It gradually decreased to disappear completely by active bone remodeling with a synchronized coordination of alkaline phosphatase-positive osteoblasts and tartrate-resistant acid phosphatase-reactive osteoclasts at post 3 months, thickening to be replaced by compact bone. Dynamic labeling showed two clear lines in the newly formed bone around the implant through this experimental period. Electron probe microanalyzer analysis demonstrated chronologically increased levels of Ca and P in the newly formed bone identical to those in the surrounding bone at post 2.5 months. These findings indicate that continuous bone remodeling after the achievement of osseointegration causes replacement of the damaged bone by compact bone as well as an improvement in bone quality. Anat Rec,292:38-47, 2009. (c) 2008 Wiley-Liss, Inc.

    DOI: 10.1002/ar.20748

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  • マウス顎関節関節腔形成過程における血管内皮細胞とマクロファージの動態

    池田順行, 井上佳世子, 鈴木晶子, 庭野将広, 高木律男, 前田健康

    日本顎関節学会雑誌   20   100   2008年7月

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  • ラット顎関節滑膜表層細胞の発育過程における筋特異型カベオリン-3タンパクの発現

    庭野 将広, 野澤 佳世子, 上, 鈴木 晶子, 池田 順行, 安島 久雄, 高木 律男, 前田 健康

    日本顎関節学会雑誌   20 ( 1 )   62 - 63   2008年4月

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    記述言語:日本語   出版者・発行元:(一社)日本顎関節学会  

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  • Immunocytochemical localization of caveolin-3 in the synoviocytes of the rat temporomandibular joint during development

    Masahiro Niwano, Kayoko Nozawa-Inoue, Akiko Suzuki, Nobuyuki Ikeda, Ritsuo Takagi, Takeyasu Maeda

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY   291 ( 3 )   233 - 241   2008年3月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Caveolins-caveolin-1, -2, -3 (Cav1, 2, 3)-are major components of caveolae, which have diverse functions. Our recent study on the temporomandibular joint (TMJ) revealed expressions of Cav1 and muscle-specific Cav3 in some synovial fibroblast-like type B cells with well-developed caveolae. However, the involvement of Cav3 expression in the differentiation and maturation of type B cells remains unclear. The present study therefore examined the chronological alterations in the localization of Cav3 in the synovial lining cells of the rat TMJ during postnatal development by immunocytochemical techniques. Observations showed immature type B cells possessed a few caveolae with Cav1 but lacked Cav3 protein at postnatal day 5 (P5). At P14, Cav3-immunopositive type B cells first appeared in the synovial lining layer. They increased in number and immunointensity from P14 to P21 as occlusion became active. In immunoelectron microscopy and double immunolabeling with heat shock protein 25 (Hsp25) and Cav3, coexpressed type B cells developed rough endoplasmic reticulum and numerous caveolae, while the Cav3-immunonegative type B cell with Hsp25 immunoreaction possessed few of these. Results suggest that Cav3 expression, which is closely related to added functional stimuli, reflects the differentiation of the type B synoviocytes.

    DOI: 10.1002/ar.20655

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  • Immunocytochemical localization of caveolin-3 in the synoviocytes of the rat temporomandibular joint during development

    Masahiro Niwano, Kayoko Nozawa-Inoue, Akiko Suzuki, Nobuyuki Ikeda, Ritsuo Takagi, Takeyasu Maeda

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY   291 ( 3 )   233 - 241   2008年3月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Caveolins-caveolin-1, -2, -3 (Cav1, 2, 3)-are major components of caveolae, which have diverse functions. Our recent study on the temporomandibular joint (TMJ) revealed expressions of Cav1 and muscle-specific Cav3 in some synovial fibroblast-like type B cells with well-developed caveolae. However, the involvement of Cav3 expression in the differentiation and maturation of type B cells remains unclear. The present study therefore examined the chronological alterations in the localization of Cav3 in the synovial lining cells of the rat TMJ during postnatal development by immunocytochemical techniques. Observations showed immature type B cells possessed a few caveolae with Cav1 but lacked Cav3 protein at postnatal day 5 (P5). At P14, Cav3-immunopositive type B cells first appeared in the synovial lining layer. They increased in number and immunointensity from P14 to P21 as occlusion became active. In immunoelectron microscopy and double immunolabeling with heat shock protein 25 (Hsp25) and Cav3, coexpressed type B cells developed rough endoplasmic reticulum and numerous caveolae, while the Cav3-immunonegative type B cell with Hsp25 immunoreaction possessed few of these. Results suggest that Cav3 expression, which is closely related to added functional stimuli, reflects the differentiation of the type B synoviocytes.

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  • ラット臼歯発生におけるカルサイクリンの発現

    河野芳朗, 河野承子, 井上佳世子, 鈴木晶子, 前田健康

    解剖学雑誌   83 ( Supplement )   183   2008年3月

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  • ラット顎関節滑膜の発育過程における筋特異型カベオリン-3タンパクの発現

    庭野 将広, 野澤 佳世子, 上, 鈴木 晶子, 高木 律男, 前田 健康

    解剖学雑誌   83 ( Suppl. )   181 - 181   2008年3月

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    記述言語:日本語   出版者・発行元:(一社)日本解剖学会  

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  • ラット顎関節滑膜の発育過程におけるカベオリン-3タンパクの発現

    庭野 将広, 野澤 佳世子, 上, 高木 律男, 前田 健康

    新潟歯学会雑誌   37 ( 2 )   251 - 251   2007年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • Bone remodeling after achievement of osseointegration by titanium implantation in rat maxillae

    M. Haga, N. Fujii, K. Nozawa-Inoue, K. Uoshima, S. Nomura, T. Maeda

    JOURNAL OF BONE AND MINERAL RESEARCH   22   S262 - S262   2007年9月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC BONE & MINERAL RES  

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  • Identification of calcyclin expression in rat amelogenesis using Acp Ddrt-per method

    Y. Kawano, S. Kinosita-Kawano, K. Nozawa-Inoue, A. Suzuki, T. Maeda

    JOURNAL OF BONE AND MINERAL RESEARCH   22   S262 - S262   2007年9月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC BONE & MINERAL RES  

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  • ラット顎関節滑膜の発育過程における筋特異型カベオリン-3タンパクの局在変化

    庭野 将広, 野澤 佳世子, 上, 鈴木 晶子, 池田 順行, 安島 久雄, 高木 律男, 前田 健康

    Journal of Oral Biosciences   49 ( Suppl. )   114 - 114   2007年8月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • Immunolocalization of aquaporin-1 in the mechanoreceptive Ruffini endings in the periodontal ligament

    Batheegama Gammacharige Tharanga Lakmali Nandasena, Akiko Suzuki, Megumi Aita, Yoshiro Kawano, Kayoko Nozawa-Inoue, Takeyasu Maeda

    BRAIN RESEARCH   1157   32 - 40   2007年7月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Previous ultrastructural studies have suggested an axon-Schwarm cell interaction in the periodontal Ruffini ending, a primary mechanoreceptor. However, no information is available on the transport mechanism between them. The present study examined the immunolocalization of aquaporin-1 (AQP1) and -4 (AQP4), a member of the water-selective channel, in the periodontal Ruffini endings of the rat incisors and trigeminal ganglion. In addition, the expression of mRNA for AQP1 and 4 was detected in the trigeminal ganglion by a RT-PCR technique. A single PCR product of the sizes anticipated for AQP1 and 4 was detectable in a reverse transcripted cDNA sample from the trigeminal ganglion, whose neurons innervate the periodontal Ruffini endings. An AQP1 immunoreaction was recognizable in the axon terminals of the periodontal Ruffini endings as well as their associated terminal Schwann cells, as confirmed with a double staining with AQP1 and either PGP9.5 or S-100 protein. However, no immunoreaction for AQP4 was found in periodontal Ruffini endings. Although the AQP4 immunoreaction was localized in some satellite cells - but never in neurons - of the trigeminal ganglion, 16.1% trigeminal neurons showed the AQP1 immunoreaction. Furthermore, the AQP1 immunoreaction was found in certain satellite cells which surrounded AQP1-positive or -negative neurons. An analysis of a cross-sectional area of these positive neurons demonstrated that approximately 66.9% of the positive neurons were 400-1000 mu m(2) (671.4 +/- 172.4 mu m(2)), indicating that they could be categorized as medium-sized neurons which mediate mechanotransduction. These findings suggest that AQP1 controls water transport in the periodontal Ruffini endings. (c) 2007 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.brainres.2007.04.033

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  • Immunolocalization of aquaporin-1 in the mechanoreceptive Ruffini endings in the periodontal ligament

    Batheegama Gammacharige Tharanga Lakmali Nandasena, Akiko Suzuki, Megumi Aita, Yoshiro Kawano, Kayoko Nozawa-Inoue, Takeyasu Maeda

    BRAIN RESEARCH   1157 ( 32-40 )   32 - 40   2007年7月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Previous ultrastructural studies have suggested an axon-Schwarm cell interaction in the periodontal Ruffini ending, a primary mechanoreceptor. However, no information is available on the transport mechanism between them. The present study examined the immunolocalization of aquaporin-1 (AQP1) and -4 (AQP4), a member of the water-selective channel, in the periodontal Ruffini endings of the rat incisors and trigeminal ganglion. In addition, the expression of mRNA for AQP1 and 4 was detected in the trigeminal ganglion by a RT-PCR technique. A single PCR product of the sizes anticipated for AQP1 and 4 was detectable in a reverse transcripted cDNA sample from the trigeminal ganglion, whose neurons innervate the periodontal Ruffini endings. An AQP1 immunoreaction was recognizable in the axon terminals of the periodontal Ruffini endings as well as their associated terminal Schwann cells, as confirmed with a double staining with AQP1 and either PGP9.5 or S-100 protein. However, no immunoreaction for AQP4 was found in periodontal Ruffini endings. Although the AQP4 immunoreaction was localized in some satellite cells - but never in neurons - of the trigeminal ganglion, 16.1% trigeminal neurons showed the AQP1 immunoreaction. Furthermore, the AQP1 immunoreaction was found in certain satellite cells which surrounded AQP1-positive or -negative neurons. An analysis of a cross-sectional area of these positive neurons demonstrated that approximately 66.9% of the positive neurons were 400-1000 mu m(2) (671.4 +/- 172.4 mu m(2)), indicating that they could be categorized as medium-sized neurons which mediate mechanotransduction. These findings suggest that AQP1 controls water transport in the periodontal Ruffini endings. (c) 2007 Elsevier B.V. All rights reserved.

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  • Involvement of neurotrophin-4/5 in regeneration of the periodontal Ruffini endings at the early stage

    Shahiqul Jabbar, Fumiko Harada, Megumi Aita, Megumi Ohishi, Isao Saito, Yoshiro Kawano, Akiko Suzuki, Kayoko Nozawa-Inoue, Takeyasu Maeda

    JOURNAL OF COMPARATIVE NEUROLOGY   501 ( 3 )   400 - 412   2007年3月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Little is known about the role of neurotrophin-4/5 (NT-4/5) in the regeneration of mechano-receptors. Therefore, the present study examined the regeneration process of Ruffini endings in the periodontal ligament in nt-4/5-deficient and wildtype mice following transection of the inferior alveolar nerve by immunohistochemistry for protein gene product 9.5 (PGP 9.5), a general neuronal marker, and by computer-assisted quantitative image analysis. Furthermore, rescue experiments by a continuous administration of recombinant NT-4/5 were performed and analyzed quantitatively. At postoperative day 3 (PO 3d), almost all PGP 9.5-positive neural elements had disappeared; they began to appear in both types of animals at PO 7d. At PO 10d, almost all nerve fibers showed a beaded appearance, with fewer ramifications in both types of mice. Although the regeneration proceeded in the wildtype, a major population of the periodontal Ruffini endings continued to display smooth outlines at PO 28d in the nt-4/5 homozygous mice. The reduction ratio of neural density reached a maximum at PO 3d, decreased at PO 10d, and later showed a plateau. In a rescue experiment, an administration of NT-4/5 showed an acceleration of nerve regeneration in the homozygous mice. These findings indicate that the nt-4/5depletion causes a delay in the regeneration of the periodontal Ruffini endings, but the delay is shortened by an exogenous administration of NT-4/5. Combined with our previous findings of bdnf-deficient mice (Harada et al. [2003] Arch Histol Cytol 66:183-194), these morphological and numerical data suggest that multiple neurotrophins such as NT-4/5 and brain-derived neurotrophic factor (BDNF) play roles in their regeneration in a stage-specific manner.

    DOI: 10.1002/cne.21256

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  • Expression of caveolin-3 in the fibroblast-like type B synoviocytes in the rat temporomandibular joint

    Kayoko Nozawa-Inoue, Akiko Suzuki, Masahiro Niwano, Yoshiro Kawano, Takeyasu Maeda

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY   290 ( 3 )   238 - 242   2007年3月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The present study revealed that the fibroblast-like type B synoviocytes (covering the surface of the synovial membrane in the rat temporomandibular joint) had muscle-specific caveolin-3 protein in their caveolae. The existence of two kinds of type B synoviocytes (with and without caveolin-3-immunoreactions even in the synovial lining layer) might reflect the functional difference between them.

    DOI: 10.1002/ar.20506

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  • Involvement of neurotrophin-4/5 in regeneration of the periodontal Ruffini endings at the early stage

    Shahiqul Jabbar, Fumiko Harada, Megumi Aita, Megumi Ohishi, Isao Saito, Yoshiro Kawano, Akiko Suzuki, Kayoko Nozawa-Inoue, Takeyasu Maeda

    JOURNAL OF COMPARATIVE NEUROLOGY   501 ( 3 )   400 - 412   2007年3月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Little is known about the role of neurotrophin-4/5 (NT-4/5) in the regeneration of mechano-receptors. Therefore, the present study examined the regeneration process of Ruffini endings in the periodontal ligament in nt-4/5-deficient and wildtype mice following transection of the inferior alveolar nerve by immunohistochemistry for protein gene product 9.5 (PGP 9.5), a general neuronal marker, and by computer-assisted quantitative image analysis. Furthermore, rescue experiments by a continuous administration of recombinant NT-4/5 were performed and analyzed quantitatively. At postoperative day 3 (PO 3d), almost all PGP 9.5-positive neural elements had disappeared; they began to appear in both types of animals at PO 7d. At PO 10d, almost all nerve fibers showed a beaded appearance, with fewer ramifications in both types of mice. Although the regeneration proceeded in the wildtype, a major population of the periodontal Ruffini endings continued to display smooth outlines at PO 28d in the nt-4/5 homozygous mice. The reduction ratio of neural density reached a maximum at PO 3d, decreased at PO 10d, and later showed a plateau. In a rescue experiment, an administration of NT-4/5 showed an acceleration of nerve regeneration in the homozygous mice. These findings indicate that the nt-4/5depletion causes a delay in the regeneration of the periodontal Ruffini endings, but the delay is shortened by an exogenous administration of NT-4/5. Combined with our previous findings of bdnf-deficient mice (Harada et al. [2003] Arch Histol Cytol 66:183-194), these morphological and numerical data suggest that multiple neurotrophins such as NT-4/5 and brain-derived neurotrophic factor (BDNF) play roles in their regeneration in a stage-specific manner.

    DOI: 10.1002/cne.21256

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  • Expression of caveolin-3 in the fibroblast-like type B synoviocytes in the rat temporomandibular joint

    Kayoko Nozawa-Inoue, Akiko Suzuki, Masahiro Niwano, Yoshiro Kawano, Takeyasu Maeda

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY   290 ( 3 )   238 - 242   2007年3月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The present study revealed that the fibroblast-like type B synoviocytes (covering the surface of the synovial membrane in the rat temporomandibular joint) had muscle-specific caveolin-3 protein in their caveolae. The existence of two kinds of type B synoviocytes (with and without caveolin-3-immunoreactions even in the synovial lining layer) might reflect the functional difference between them.

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  • Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings

    Yasushi Igarashi, Megumi Aita, Akiko Suzuki, Tharanga Nandasena, Yoshiro Kawano, Kayoko Nozawa-Inoue, Takeyasu Maeda

    NEUROSCIENCE LETTERS   412 ( 3 )   222 - 226   2007年2月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    Our recent study revealed an intense immunoreaction for GDNF and its receptors in the Ruffini endings, primary mechanoreceptors in the periodontal ligament, of young rats. However, no information is available for the expression of GDNF and its receptors during their development. The present study aimed to reveal postnatal changes in the immuno-expression of GDNF, GFR alpha l and RET in the periodontal Ruffini endings of the rat incisors by double immunofluorescent staining. At postnatal day 3 (PO 3d), no structure with GDNF-, GFR alpha l-, or RET-immunoreaction existed in the periodontal ligament. The PGP 9.5-positive nerve fibers without GDNF- and RET-immunoreaction displayed a dendritic fashion at PO lw, with a GFR alpha l-reaction found around these nerves. At PO 2w, GDNF-positive terminal Schwann cells occurred near the thick and dendritic axons, a part of which showed a RET-reaction, with no reactive cells near the thin nerves. The terminal Schwann cells became positive for GFR alpha l, but lacked RET-immunoreaction. At PO 3w, when the formation of the periodontal Ruffini endings had proceeded, GDNF-positive terminal Schwann cells began to increase in number. This stage-specific immuno-expression pattern suggests that GDNF is a key molecule for the maturation and maintenance of the periodontal Ruffini endings. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2006.11.012

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  • Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings

    Yasushi Igarashi, Megumi Aita, Akiko Suzuki, Tharanga Nandasena, Yoshiro Kawano, Kayoko Nozawa-Inoue, Takeyasu Maeda

    NEUROSCIENCE LETTERS   412 ( 3 )   222 - 226   2007年2月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    Our recent study revealed an intense immunoreaction for GDNF and its receptors in the Ruffini endings, primary mechanoreceptors in the periodontal ligament, of young rats. However, no information is available for the expression of GDNF and its receptors during their development. The present study aimed to reveal postnatal changes in the immuno-expression of GDNF, GFR alpha l and RET in the periodontal Ruffini endings of the rat incisors by double immunofluorescent staining. At postnatal day 3 (PO 3d), no structure with GDNF-, GFR alpha l-, or RET-immunoreaction existed in the periodontal ligament. The PGP 9.5-positive nerve fibers without GDNF- and RET-immunoreaction displayed a dendritic fashion at PO lw, with a GFR alpha l-reaction found around these nerves. At PO 2w, GDNF-positive terminal Schwann cells occurred near the thick and dendritic axons, a part of which showed a RET-reaction, with no reactive cells near the thin nerves. The terminal Schwann cells became positive for GFR alpha l, but lacked RET-immunoreaction. At PO 3w, when the formation of the periodontal Ruffini endings had proceeded, GDNF-positive terminal Schwann cells began to increase in number. This stage-specific immuno-expression pattern suggests that GDNF is a key molecule for the maturation and maintenance of the periodontal Ruffini endings. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

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  • 歯根膜ルフィニ神経終末の発生過程におけるGDNFおよび同受容体の発現

    相田恵, 鈴木晶子, タランガ ナンダセーナ, 井上佳世子, 前田健康

    J Oral Biosci   48 ( Supplement )   125   2006年9月

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  • Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint

    A Suzuki, K Nozawa-Inoue, N Amizuka, K Ono, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   288A ( 6 )   646 - 652   2006年6月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Previous studies have pointed out a lack of adhesion structures in the synovial lining layer of the rat temporomandibular joint (TMJ) despite showing an epithelial arrangement. CD44, a major cell adhesion molecule, plays crucial roles as an anchor between cells and extracellular matrices by binding hyaluronan (HA) for the development of organs or the metastasis of tumors. The present study examined the localization of CD44 in the synovial membrane of the rat TMJ by immunocytochemistry for OX50, ED1, and Hsp25, which are markers for the rat CD44, macrophage-like type A, and fibroblast-like type B synoviocytes, respectively. Histochemistry for HA-binding protein (HABP) was also employed for the detection of HA. OX50 immunoreactions were found along the cell surface and, in particular, accumulated along the surface of the articular cavity. Observations by a double immunostaining and immunoelectron microscopy revealed that all the OX50-immunopositive cells were categorized as fibroblastic type B cells, which had many caveolae and a few vesicles reactive to intense OX50. However, the macrophage-like type A cells did not have any OX50 immunoreaction in the synovial lining layer. A strong HABP reaction was discernable in the extracellular matrix surrounding both OX50-positive and -negative cells in the synovial lining layers, exhibiting a meshwork distribution, but weak in its sublining layer. This localization pattern of CD44 and HABP might be involved in the formation of the epithelial arrangement of the synovial lining layer. Furthermore, OX50 immunonegativity in the type A cells suggests their low phagocytotic activity in the rat TMJ under normal conditions.

    DOI: 10.1002/ar.a.20331

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  • Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint

    A Suzuki, K Nozawa-Inoue, N Amizuka, K Ono, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   288A ( 6 )   646 - 652   2006年6月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Previous studies have pointed out a lack of adhesion structures in the synovial lining layer of the rat temporomandibular joint (TMJ) despite showing an epithelial arrangement. CD44, a major cell adhesion molecule, plays crucial roles as an anchor between cells and extracellular matrices by binding hyaluronan (HA) for the development of organs or the metastasis of tumors. The present study examined the localization of CD44 in the synovial membrane of the rat TMJ by immunocytochemistry for OX50, ED1, and Hsp25, which are markers for the rat CD44, macrophage-like type A, and fibroblast-like type B synoviocytes, respectively. Histochemistry for HA-binding protein (HABP) was also employed for the detection of HA. OX50 immunoreactions were found along the cell surface and, in particular, accumulated along the surface of the articular cavity. Observations by a double immunostaining and immunoelectron microscopy revealed that all the OX50-immunopositive cells were categorized as fibroblastic type B cells, which had many caveolae and a few vesicles reactive to intense OX50. However, the macrophage-like type A cells did not have any OX50 immunoreaction in the synovial lining layer. A strong HABP reaction was discernable in the extracellular matrix surrounding both OX50-positive and -negative cells in the synovial lining layers, exhibiting a meshwork distribution, but weak in its sublining layer. This localization pattern of CD44 and HABP might be involved in the formation of the epithelial arrangement of the synovial lining layer. Furthermore, OX50 immunonegativity in the type A cells suggests their low phagocytotic activity in the rat TMJ under normal conditions.

    DOI: 10.1002/ar.a.20331

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  • 生後マウスにおける下顎頭軟骨の経時的観察

    池田順行, HOSSAIN Kazi Sazzad, 井上佳世子, 鈴木晶子, 高木律男, 前田健康, 網塚憲生

    日本顎関節学会雑誌   18 ( 1 )   42   2006年4月

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    記述言語:日本語  

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  • Histochemical evidence of osteoclastic degradation of extracellular matrix in osteolytic metastasis originating from human lung small carcinoma (SBC-5) cells

    MQ Li, N Amizuka, K Takeuchi, PHL Freitas, Y Kawano, M Hoshino, K Oda, K Nozawa-Inoue, T Maeda

    MICROSCOPY RESEARCH AND TECHNIQUE   69 ( 2 )   73 - 83   2006年2月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The aim of this study was to assess the dynamics of osteoclast migration and the degradation of unmineralized extracellular matrix in an osteolytic metastasis by examining a well-standardized lung cancer metastasis model of nude mice. SBC-5 human lung small carcinoma cells were injected into the left cardiac ventricle of 6-week-old BALB/c nu/nu mice under anesthesia. At 25-30 days after injection, the animals were sacrificed and their femora and/or tibiae were removed for histochemical analyses. Metastatic lesions were shown to occupy a considerable area extending from the metaphyses to the bone marrow region. Tartrate resistant acid phosphatase (TRAPase)-positive osteoclasts were found in association with an alkaline phosphatase (ALPase)positive osteoblastic layer lining the bone surface, but could also be localized in the ALPase-negative stromal tissues that border the tumor nodules. These stromal tissues were markedly positive for osteopontin, and contained a significant number of TRAPase-positive osteoclasts expressing immunoreactivity for CD44. We thus speculated that, mediating its affinity for CD44, osteopontin may serve to facilitate osteoclastic migration after their formation associated with ALPase-positive osteoblasts. We next examined the localization of cathepsin K and matrix metallo-proteinase-9 (MMP-9) in osteoclasts. Osteoclasts adjacent to the bone surfaces were positive for both proteins, whereas those in the stromal tissues in the tumor nests showed only MMP-9 immunoreactivity. Immunoelectron microscopy disclosed the presence of MMP-9 in the Golgi apparatus and in vesicular structures at the baso-lateral cytoplasmic region of the osteoclasts found in the stromal tissue. MMP-9-positive vesicular structures also contained fragmented extracellular materials. Thus, osteoclasts appear to either select an optimized function, namely secreting proteolytic enzymes from ruffled borders during bone resorption, or recognize the surrounding extracellular matrix by mediating osteopontin/CD44 interaction, and internalize the extracellular matrices.

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  • Histochemical evidence of osteoclastic degradation of extracellular matrix in osteolytic metastasis originating from human lung small carcinoma (SBC-5) cells

    MQ Li, N Amizuka, K Takeuchi, PHL Freitas, Y Kawano, M Hoshino, K Oda, K Nozawa-Inoue, T Maeda

    MICROSCOPY RESEARCH AND TECHNIQUE   69 ( 2 )   73 - 83   2006年2月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The aim of this study was to assess the dynamics of osteoclast migration and the degradation of unmineralized extracellular matrix in an osteolytic metastasis by examining a well-standardized lung cancer metastasis model of nude mice. SBC-5 human lung small carcinoma cells were injected into the left cardiac ventricle of 6-week-old BALB/c nu/nu mice under anesthesia. At 25-30 days after injection, the animals were sacrificed and their femora and/or tibiae were removed for histochemical analyses. Metastatic lesions were shown to occupy a considerable area extending from the metaphyses to the bone marrow region. Tartrate resistant acid phosphatase (TRAPase)-positive osteoclasts were found in association with an alkaline phosphatase (ALPase)positive osteoblastic layer lining the bone surface, but could also be localized in the ALPase-negative stromal tissues that border the tumor nodules. These stromal tissues were markedly positive for osteopontin, and contained a significant number of TRAPase-positive osteoclasts expressing immunoreactivity for CD44. We thus speculated that, mediating its affinity for CD44, osteopontin may serve to facilitate osteoclastic migration after their formation associated with ALPase-positive osteoblasts. We next examined the localization of cathepsin K and matrix metallo-proteinase-9 (MMP-9) in osteoclasts. Osteoclasts adjacent to the bone surfaces were positive for both proteins, whereas those in the stromal tissues in the tumor nests showed only MMP-9 immunoreactivity. Immunoelectron microscopy disclosed the presence of MMP-9 in the Golgi apparatus and in vesicular structures at the baso-lateral cytoplasmic region of the osteoclasts found in the stromal tissue. MMP-9-positive vesicular structures also contained fragmented extracellular materials. Thus, osteoclasts appear to either select an optimized function, namely secreting proteolytic enzymes from ruffled borders during bone resorption, or recognize the surrounding extracellular matrix by mediating osteopontin/CD44 interaction, and internalize the extracellular matrices.

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  • Expression of caveolin-1 in the rat temporomandibular joint

    K Nozawa-Inoue, A Suzuki, N Amizuka, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   288A ( 1 )   8 - 12   2006年1月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    This immunocytochemical study revealed the expression of caveolin-1, a major protein of caveolae, in the rat temporomandibular joint. In the synovial lining layer, immunoreactive products for caveolin-1 were detected on the cell membrane of the fibroblast-like type B cells, as confirmed by immunocytochemistry for heat shock protein 25. The cells in the articular disk, the articular layer, and zone of proliferation of the mandibular condyle also showed intense immunoreactions for caveolin-1. (C) 2005 Wiley-Liss, Inc.

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  • Expression of caveolin-1 in the rat temporomandibular joint

    K Nozawa-Inoue, A Suzuki, N Amizuka, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   288A ( 1 )   8 - 12   2006年1月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    This immunocytochemical study revealed the expression of caveolin-1, a major protein of caveolae, in the rat temporomandibular joint. In the synovial lining layer, immunoreactive products for caveolin-1 were detected on the cell membrane of the fibroblast-like type B cells, as confirmed by immunocytochemistry for heat shock protein 25. The cells in the articular disk, the articular layer, and zone of proliferation of the mandibular condyle also showed intense immunoreactions for caveolin-1. (C) 2005 Wiley-Liss, Inc.

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  • 顎関節関節腔形成における血管内皮細胞とマクロファージの動態

    鈴木 晶子, 野澤 佳世子, 上, 網塚 憲生, 前田 健康

    新潟歯学会雑誌   35 ( 2 )   246 - 246   2006年1月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • Synovial lining cells in the temporomandibular joint.

    Nozawa-Inoue K

    J. Oral Biosci.   48 ( 3 )   198 - 209   2006年

  • Development of the articular cavity in the rat temporomandibular joint with special reference to the behavior of endothelial cells and macrophages

    A Suzuki, K Nozawa-Inoue, N Ikeda, N Amizuka, K Ono, R Takagi, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   286A ( 2 )   908 - 916   2005年10月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Previous developmental studies on the temporomandibular joint (TMJ) have proposed several hypotheses on the formation of its articular cavity. However, detailed information is meager. The present study examined the formation process of the articular cavity in the rat TMJ by immunocytochemistry for CD31, RECA-1, and ED1, which are useful cellular markers for endothelial cells and monocyte/macrophage lineages, respectively. The upper articular cavity formation had begun by embryonic day 21 (E21) and was completed at postnatal day 1 (P1) in advance of the lower cavitation; the latter took place from P1 to P3. The occurrence and distribution pattern of the CD31-, RECA-1-, and ED1-positive cells differed between the upper and lower articular cavity-forming areas: the ED1-positive cells exclusively occurred in the area of the prospective upper articular cavity prior to its formation, while no ED1-positive cell appeared in the lower cavity-forming area. In contrast, the CD31- and RECA-1-positive endothelial cells were restricted to the lower cavity-forming area (never the prospective upper cavity) at E19 and diminished thereafter. Throughout the cavity formation, we failed to find any apoptotic cells in the cavity formation area, indicating no involvement of apoptosis in the cavity formation in TMJ. The present findings on the behaviors of endothelial cells and ED1-positive cells show a possibility of different mechanism in the cavity formation between the upper and lower articular cavities in the rat TMJ. The appearance of ED1-reactive cells and temporal vascularization may play crucial roles in the upper and lower articular cavity formation, respectively. (C) 2005 Wiley-Liss, Inc.

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  • Development of the articular cavity in the rat temporomandibular joint with special reference to the behavior of endothelial cells and macrophages

    A Suzuki, K Nozawa-Inoue, N Ikeda, N Amizuka, K Ono, R Takagi, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   286A ( 2 )   908 - 916   2005年10月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Previous developmental studies on the temporomandibular joint (TMJ) have proposed several hypotheses on the formation of its articular cavity. However, detailed information is meager. The present study examined the formation process of the articular cavity in the rat TMJ by immunocytochemistry for CD31, RECA-1, and ED1, which are useful cellular markers for endothelial cells and monocyte/macrophage lineages, respectively. The upper articular cavity formation had begun by embryonic day 21 (E21) and was completed at postnatal day 1 (P1) in advance of the lower cavitation; the latter took place from P1 to P3. The occurrence and distribution pattern of the CD31-, RECA-1-, and ED1-positive cells differed between the upper and lower articular cavity-forming areas: the ED1-positive cells exclusively occurred in the area of the prospective upper articular cavity prior to its formation, while no ED1-positive cell appeared in the lower cavity-forming area. In contrast, the CD31- and RECA-1-positive endothelial cells were restricted to the lower cavity-forming area (never the prospective upper cavity) at E19 and diminished thereafter. Throughout the cavity formation, we failed to find any apoptotic cells in the cavity formation area, indicating no involvement of apoptosis in the cavity formation in TMJ. The present findings on the behaviors of endothelial cells and ED1-positive cells show a possibility of different mechanism in the cavity formation between the upper and lower articular cavities in the rat TMJ. The appearance of ED1-reactive cells and temporal vascularization may play crucial roles in the upper and lower articular cavity formation, respectively. (C) 2005 Wiley-Liss, Inc.

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  • ラット顎関節におけるカベオリン-1の局在

    野澤 佳世子, 井上, 鈴木 晶子, 網塚 憲生, 前田 健康

    Journal of Oral Biosciences   47 ( Suppl. )   102 - 102   2005年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 顎関節滑膜におけるCD44の局在

    鈴木 晶子, 野澤 佳世子, 上, 網塚 憲生, 前田 健康

    Journal of Oral Biosciences   47 ( Suppl. )   102 - 102   2005年9月

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  • Histochemical evidences on the chronological alterations of the hypertrophic zone of mandibular condylar cartilage

    KS Hossain, N Amizuka, N Ikeda, K Nozawa-Inoue, A Suzuki, MQ Li, K Takeuchi, M Aita, Y Kawano, M Hoshino, K Oda, R Takagi, T Maeda

    MICROSCOPY RESEARCH AND TECHNIQUE   67 ( 6 )   325 - 335   2005年8月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The hypertrophic chondrocytes lack the ability to proliferate, thus permitting matrix mineralization as well as vascular invasion from the bone in both the mandibular condyle and the epiphyseal cartilage. This study attempted to verify whether the histological appearance of the hypertrophic chondrocytes is in a steady state during postnatal development of the mouse mandibular condyle. Type X collagen immunohistochemistry apparently distinguished the fibrous layer described previously as the "articular zone," "articular layer," and "resting zone" from the hypertrophic zone. Interestingly, the ratio of the type X collagen-positive hypertrophic zone in the entire condyle seemed higher in the early stages but decreased in the later stages. Some apparently compacted cells in the hypertrophic zone showed proliferating cell nuclear antigen (PCNA) immunoreaction, indicating the potential for cell proliferation at the early stages. As the mice matured, in contrast, they further enlarged and assumed typical features of hypertrophic chondrocytes. Apoptotic cells were also discernible in the hypertrophic zone at the early but not later stages. Consistent with morphological configurations of hypertrophic chondrocytes, immunoreactions for alkaline phosphatase, osteopontin, and type I collagen were prominent at the later stage, but not the early stage. Cartilaginous matrices demonstrated scattered patches of mineralization at the early stage, but increased in their volume and connectivity at the later stage. Thus, the spatial and temporal occurrence of these immunoreactions as well as apoptosis likely reflect the prematurity of hypertrophying cells at the early stage, and imply a physiological relevance during the early development of the mandibular condyles.

    DOI: 10.1002/jemt.20211

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  • Histochemical evidences on the chronological alterations of the hypertrophic zone of mandibular condylar cartilage

    KS Hossain, N Amizuka, N Ikeda, K Nozawa-Inoue, A Suzuki, MQ Li, K Takeuchi, M Aita, Y Kawano, M Hoshino, K Oda, R Takagi, T Maeda

    MICROSCOPY RESEARCH AND TECHNIQUE   67 ( 6 )   325 - 335   2005年8月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The hypertrophic chondrocytes lack the ability to proliferate, thus permitting matrix mineralization as well as vascular invasion from the bone in both the mandibular condyle and the epiphyseal cartilage. This study attempted to verify whether the histological appearance of the hypertrophic chondrocytes is in a steady state during postnatal development of the mouse mandibular condyle. Type X collagen immunohistochemistry apparently distinguished the fibrous layer described previously as the "articular zone," "articular layer," and "resting zone" from the hypertrophic zone. Interestingly, the ratio of the type X collagen-positive hypertrophic zone in the entire condyle seemed higher in the early stages but decreased in the later stages. Some apparently compacted cells in the hypertrophic zone showed proliferating cell nuclear antigen (PCNA) immunoreaction, indicating the potential for cell proliferation at the early stages. As the mice matured, in contrast, they further enlarged and assumed typical features of hypertrophic chondrocytes. Apoptotic cells were also discernible in the hypertrophic zone at the early but not later stages. Consistent with morphological configurations of hypertrophic chondrocytes, immunoreactions for alkaline phosphatase, osteopontin, and type I collagen were prominent at the later stage, but not the early stage. Cartilaginous matrices demonstrated scattered patches of mineralization at the early stage, but increased in their volume and connectivity at the later stage. Thus, the spatial and temporal occurrence of these immunoreactions as well as apoptosis likely reflect the prematurity of hypertrophying cells at the early stage, and imply a physiological relevance during the early development of the mandibular condyles.

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  • Immunocytochemical localization of MAPKAPK-2 and Hsp25 in the rat temporomandibular joint

    K Nozawa-Inoue, N Amizuka, A Suzuki, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   284A ( 2 )   522 - 528   2005年6月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    One series of our research has shown an intense expression of immuno-reaction for heat shock protein 25 (Hsp25) in various cellular elements in the rat temporomandibular joint (TMJ). This protein is the major substrate of mitogen-activated protein kinase-activated protein kinase-2 (MAPKAPK-2), which mediates an intracellular stress-activated signaling pathway to stimulate cytosolic actin reorganization under various stresses. The present study was undertaken to examine the localization of MAPKAPK-2 in the rat TMJ by immunocytochemical techniques. Furthermore, confocal microscopy with double staining was employed to demonstrate the colocalization of MAPKAPK-2 and Hsp25. Immunocytochemistry for MAPKAPK-2 showed an intense immunoreaction in the cytoplasm of the synovial lining cells, the endothelial cells, and the fibroblasts in the synovial membrane of the rat TMJ. Double immunostaining under a confocal microscope succeeded in demonstrating the colocalization of MAPKAPK-2 and Hsp25 immunoreactions in the cytoplasm of fibroblastic type B synoviocytes in the TMJ. On the other hand, the macrophage-like type A-cells expressed MAPKAPK-2 immunoreactions but lacked Hsp25 immunoreactivity. The cells in the articular disk and the chondrocytes in the maturative and hypertrophic layer of the mandibular cartilage also showed intense immunoreactions for MAPKAPK-2 and Hsp25. In addition to cytoplasmic localization, MAPKAPK-2 immunoreactions were found in the nucleus of some synovial lining cells, cells in the articular disk, and chondrocytes. Current observations imply the presence of the phosphorylation of Hsp25 via activated MAPKAPK-2 in the cytoplasm. MAPKAPK-2 and Hsp25 possibly participate in the induction of cytoskeletal changes to the various cellular elements in rat TMJ under normal conditions. © 2005 Wiley-Liss, Inc.

    DOI: 10.1002/ar.a.20191

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  • Immunocytochemical localization of MAPKAPK-2 and Hsp25 in the rat temporomandibular joint

    K Nozawa-Inoue, N Amizuka, A Suzuki, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   284A ( 2 )   522 - 528   2005年6月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    One series of our research has shown an intense expression of immuno-reaction for heat shock protein 25 (Hsp25) in various cellular elements in the rat temporomandibular joint (TMJ). This protein is the major substrate of mitogen-activated protein kinase-activated protein kinase-2 (MAPKAPK-2), which mediates an intracellular stress-activated signaling pathway to stimulate cytosolic actin reorganization under various stresses. The present study was undertaken to examine the localization of MAPKAPK-2 in the rat TMJ by immunocytochemical techniques. Furthermore, confocal microscopy with double staining was employed to demonstrate the colocalization of MAPKAPK-2 and Hsp25. Immunocytochemistry for MAPKAPK-2 showed an intense immunoreaction in the cytoplasm of the synovial lining cells, the endothelial cells, and the fibroblasts in the synovial membrane of the rat TMJ. Double immunostaining under a confocal microscope succeeded in demonstrating the colocalization of MAPKAPK-2 and Hsp25 immunoreactions in the cytoplasm of fibroblastic type B synoviocytes in the TMJ. On the other hand, the macrophage-like type A-cells expressed MAPKAPK-2 immunoreactions but lacked Hsp25 immunoreactivity. The cells in the articular disk and the chondrocytes in the maturative and hypertrophic layer of the mandibular cartilage also showed intense immunoreactions for MAPKAPK-2 and Hsp25. In addition to cytoplasmic localization, MAPKAPK-2 immunoreactions were found in the nucleus of some synovial lining cells, cells in the articular disk, and chondrocytes. Current observations imply the presence of the phosphorylation of Hsp25 via activated MAPKAPK-2 in the cytoplasm. MAPKAPK-2 and Hsp25 possibly participate in the induction of cytoskeletal changes to the various cellular elements in rat TMJ under normal conditions. © 2005 Wiley-Liss, Inc.

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  • 骨吸収型骨転移病巣で破骨細胞が産生するcathepsin KとMMP-9の免疫局在

    李 敏啓, 網塚 憲生, 井上 佳世子, 河野 芳郎, 竹内 亀一, 前田 健康

    解剖学雑誌   80 ( Suppl. )   161 - 161   2005年3月

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    記述言語:日本語   出版者・発行元:(一社)日本解剖学会  

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  • Ultrastructural Images of Enamel Tufts in Human Permanent Teeth

    Norio Amizuka, Takashi Uchida, Kayoko Nozawa-Inoue, Yoshiro Kawano, Akiko Suzuki, Minqi Li, Makiko Nasu, Taku Kojima, Naoko Sakagami, Hidehiro Ozawa, Takeyasu Maeda

    Journal of Oral Biosciences   47 ( 1 )   33 - 41   2005年

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    記述言語:英語  

    Human enamel tufts appeared as corrugated ribbon-like structures located on the dentin parallel to the tooth axis when observed under the binocular microscope and scanning electron microscope (SEM). SEM observation disclosed enamel tufts as bundles of well-extended tubular structures attributable to hypomineralized enamel sheaths. Plate-like structures, previously referred to as “tuft-root” ran in the center of the enamel tufts, connecting the dentin surface. When observing under the transmission electron microscope, the plates of tufts extended from the superficial layer of the dentin, penetrating the hypermineralized zone adjacent to the dentin-enamel (D-E) junction, and then, reaching the tuft region. In the tuft region, the plates of tufts ran mainly along the enamel sheaths and partially across the enamel prisms. The immuno-gold technique verified an intense immunoreactivity for amelogenin in the superficial layer of the dentin as well as the enamel prisms in the tufts, although no reaction was found over the “plates of tufts”. The immunoreactivity for 13–17 kd sheath proteins, also denoted as sheathlin, ameloblastin or amelin, was detected over the filamentous structures closely associated with the enamel sheaths in the enamel tuft. Thus, our study demonstrated that enamel tufts consist of both well extended hypomineralized enamel prisms and “plates of tufts”. The major organic substance of the enamel tufts is suggested to be 13–17 kd sheath proteins rather than amelogenin. © 2005, Japanese Association for Oral Biology. All rights reserved.

    DOI: 10.2330/joralbiosci.47.33

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  • Synovial Lining Cells in the Temporomandibular Joint

    Nozawa-Inoue Kayoko

    journal of oral biosciences   48 ( 3 )   198 - 209   2005年

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    記述言語:英語  

    The synovial membrane in the temporomandibular joint (TMJ) consists of a lining and connective tissue sublining layers. Its synovial lining layer contains two types of synovial lining cells-macrophage-like type A and fibroblast-like type B cells. Ultrastructurally, the type A cell is characterized by numerous vacuoles, lysosomes, nuclei with rich heterochromatin, and cell surface filopodia while the type B cell possesses a well-developed rough endoplasmic reticulum, a nucleus with less chromatin, cytoplasmic projections, and cell surface caveolae. However, they are hardly distinguishable under the light microscope without specific cell markers. Immunocytochemistry and in situ hybridization histochemistry have revealed that fibroblast-like type B cells in the murine TMJ show an intense reaction for 25 kDa-heat shock protein (Hsp25), indicating that this protein is a useful cell marker for the type B cells. On the other hand, the macrophage-like type A cells react with ED1, which recognizes the macrophage/monocyte lineage because the type A cells are derived from monocyte. Double immunostaining for Hsp25 and ED1 demonstrated regional differences in the population of the lining cells among the synovial membrane of rat TMJ-the type A cell is predominant in the anterior portion while the type B cell is abundant in the posterior synovial folds. Hsp25 protein is the major substrate of mitogen-activated protein kinase-activated protein kinase-2 (MAPKAPK-2), which mediates a stress-activated signaling pathway. The activation of Hsp25 by MAPKAPK-2 has been considered to regulate cytoskeletal actin organization, resulting in cellular morphological changes. MAPKAPK-2 and Hsp25 are co-localized in the cytoplasm of the fibroblast-like type B cells in the rat TMJ, implying that these proteins possibly participate in the induction of morphological changes of the type B cells. © 2006, Japanese Association for Oral Biology. All rights reserved.

    DOI: 10.2330/joralbiosci.48.198

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  • Ultrastructural Images of Enamel Tufts in Human Permanent Teeth

    Norio Amizuka, Takashi Uchida, Kayoko Nozawa-Inoue, Yoshiro Kawano, Akiko Suzuki, Minqi Li, Makiko Nasu, Taku Kojima, Naoko Sakagami, Hidehiro Ozawa, Takeyasu Maeda

    Journal of Oral Biosciences   47 ( 1 )   33 - 41   2005年

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    記述言語:英語  

    Human enamel tufts appeared as corrugated ribbon-like structures located on the dentin parallel to the tooth axis when observed under the binocular microscope and scanning electron microscope (SEM). SEM observation disclosed enamel tufts as bundles of well-extended tubular structures attributable to hypomineralized enamel sheaths. Plate-like structures, previously referred to as “tuft-root” ran in the center of the enamel tufts, connecting the dentin surface. When observing under the transmission electron microscope, the plates of tufts extended from the superficial layer of the dentin, penetrating the hypermineralized zone adjacent to the dentin-enamel (D-E) junction, and then, reaching the tuft region. In the tuft region, the plates of tufts ran mainly along the enamel sheaths and partially across the enamel prisms. The immuno-gold technique verified an intense immunoreactivity for amelogenin in the superficial layer of the dentin as well as the enamel prisms in the tufts, although no reaction was found over the “plates of tufts”. The immunoreactivity for 13–17 kd sheath proteins, also denoted as sheathlin, ameloblastin or amelin, was detected over the filamentous structures closely associated with the enamel sheaths in the enamel tuft. Thus, our study demonstrated that enamel tufts consist of both well extended hypomineralized enamel prisms and “plates of tufts”. The major organic substance of the enamel tufts is suggested to be 13–17 kd sheath proteins rather than amelogenin. © 2005, Japanese Association for Oral Biology. All rights reserved.

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  • Immunohistochemical localization of periostin in tooth and its surrounding tissues in mouse mandibles during development

    H Suzuki, N Amizuka, Kii, I, Y Kawano, K Nozawa-Inoue, A Suzuki, H Yoshie, A Kudo, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   281A ( 2 )   1264 - 1275   2004年12月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Previous reports have shown expression of immunoreactivity for periostin, originally identified as osteoblast-specific factor-2, in the periosteum and periodontal ligament. However, the developmental changes in its expression and the detailed immunolocalization have remained veiled. The present study was undertaken to examine the spatiotemporal expression of this protein in teeth and their associated tissues of mice during development at light and electron microscopic levels. In tooth germs at cap stage, periostin immunoreactivity was recognizable in the interface between inner enamel epithelium and preodontoblasts as well as in the mesenchymal tissues around cervical loop. Dental follicles around tooth germs at bell stage localized periostin immunopositivity in addition to the immunopositive areas observed in cap-staged tooth germs, although the functional significance of periostin has remained unclear in tooth development. Furthermore, periostin immunoreactivity was also found in the alveolar bone surface. In the incisors of both 7- and 21-day-old mice, immunoreaction for periostin was discernible in the lingual periodontal ligament and labial fibrous tissue adjacent to the papillary layer. After postnatal day 7, immunoreaction for periostin came to be restricted to the fibrous bundles in the periodontal ligament in accordance with the organization of the periodontal fibers, indicating its localization matched the morphogenesis of the periodontal ligament. Immunoelectron microscopic observation of the mature periodontal ligament verified the localization of periostin between the cytoplasmic processes of periodontal fibroblasts and cementoblasts and the adjacent collagen fibrils. Our findings suggest that periostin is involved at the sites of the cell-to-matrix interaction, serving as adhesive equipment for bearing mechanical forces, including occlusal force and tooth eruption. (C) 2004 Wiley-Liss, Inc.

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  • Immunohistochemical localization of periostin in tooth and its surrounding tissues in mouse mandibles during development

    H Suzuki, N Amizuka, Kii, I, Y Kawano, K Nozawa-Inoue, A Suzuki, H Yoshie, A Kudo, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   281A ( 2 )   1264 - 1275   2004年12月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    Previous reports have shown expression of immunoreactivity for periostin, originally identified as osteoblast-specific factor-2, in the periosteum and periodontal ligament. However, the developmental changes in its expression and the detailed immunolocalization have remained veiled. The present study was undertaken to examine the spatiotemporal expression of this protein in teeth and their associated tissues of mice during development at light and electron microscopic levels. In tooth germs at cap stage, periostin immunoreactivity was recognizable in the interface between inner enamel epithelium and preodontoblasts as well as in the mesenchymal tissues around cervical loop. Dental follicles around tooth germs at bell stage localized periostin immunopositivity in addition to the immunopositive areas observed in cap-staged tooth germs, although the functional significance of periostin has remained unclear in tooth development. Furthermore, periostin immunoreactivity was also found in the alveolar bone surface. In the incisors of both 7- and 21-day-old mice, immunoreaction for periostin was discernible in the lingual periodontal ligament and labial fibrous tissue adjacent to the papillary layer. After postnatal day 7, immunoreaction for periostin came to be restricted to the fibrous bundles in the periodontal ligament in accordance with the organization of the periodontal fibers, indicating its localization matched the morphogenesis of the periodontal ligament. Immunoelectron microscopic observation of the mature periodontal ligament verified the localization of periostin between the cytoplasmic processes of periodontal fibroblasts and cementoblasts and the adjacent collagen fibrils. Our findings suggest that periostin is involved at the sites of the cell-to-matrix interaction, serving as adhesive equipment for bearing mechanical forces, including occlusal force and tooth eruption. (C) 2004 Wiley-Liss, Inc.

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  • ラット顎関節関節腔の形成における血管新生の関与

    鈴木 晶子, 野澤-井上, 佳世子, 池田 順行, 網塚 憲生, 前田 健康

    Journal of oral biosciences   46 ( 5 )   406 - 406   2004年9月

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    記述言語:日本語   出版者・発行元:歯科基礎医学会  

    CiNii Article

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  • 発生期マウス下顎関節突起における肥大層の組織化学的変化(Histochemical changes of the hypertrophic zone in developing mouse mandibular condyle)

    Hossain Kazi Sazzad, 池田 順行, 野澤 佳世子, 網塚 憲生, 織田 公光, 高木 律男, 前田 健康

    Journal of Oral Biosciences   46 ( 5 )   396 - 396   2004年9月

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    記述言語:英語   出版者・発行元:(一社)歯科基礎医学会  

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  • Development of the synovial membrane in the rat temporomandibular joint as demonstrated by immunocytochemistry for heat shock protein 25

    N Ikeda, K Nozawa-Inoue, R Takagi, T Maeda

    ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY   279A ( 1 )   623 - 635   2004年7月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The synovial lining layer of the temporomandibular joint (TMJ) consists of macrophage-like type A cells and fibroblast-like type B cells. Until now, little information has been available on the development of the synovial membrane in TMJ. In the present study we examined the development of the synovial lining layer in the rat TMJ by light- and electron-microscopic immunocytochemistry for heat shock protein (Hsp) 25, which is a useful marker for type B cells. At embryonic day 19 (E19), a few Hsp25-positive cells first appeared in the upper portion of the developing condyle. During the formation of the upper articular cavity (E21 to postnatal day 1 (P1)), a few positive cells were arranged on its surface. Immunoelectron microscopy demonstrated that these cells had ultrastructural features of fibroblast-like type B cells. In addition, some Hsp25-positive cells moved to the deep portion by extending their cytoplasmic processes toward the articular cavity at P3. At that time, the presence of typical macrophage-like type A cells in the lining layer was confirmed by immunoelectron microscopy. The slender processes of Hsp25-positive cells showed a continuous covering with the synovial surface at P7, followed by a drastic increase in the Hsp25-positive cells at P15 and later, when active jaw movement occurred. These findings suggested that the arrangement and morphological maturation of type B cells are closely related to the formation of the articular cavity in the embryonic period and the commencement of active jaw movement after birth, respectively. (C) 2004 Wiley-Liss, Inc.

    DOI: 10.1002/ar.a.20043

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  • ラット顎関節におけるエストロゲン受容体αの局在

    野澤 佳世子, 井上, 網塚 憲生, 前田 健康, 山田 一穂, 小澤 英浩

    THE BONE   18 ( 3 )   251 - 255   2004年5月

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    記述言語:日本語   出版者・発行元:(株)メディカルレビュー社  

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  • ラット顎関節滑膜表層B型細胞の発生

    池田 順行, 野澤 佳世子, 上, 高木 律男, 前田 健康

    日本顎関節学会雑誌   16 ( 1 )   39 - 39   2004年4月

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    記述言語:日本語   出版者・発行元:(一社)日本顎関節学会  

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  • ラット顎関節滑膜B型表層細胞の発生過程

    池田 順行, 野澤 佳世子, 上, 高木 律男, 前田 健康

    新潟歯学会雑誌   33 ( 2 )   296 - 297   2004年1月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • Development of the synovial membrane in the rat temporomandibular joint as demonstrated by immunocytochemistry for heat shock protein 25.

    Anat. Rec.   279A: 623-635   2004年

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  • Synovial Type B Cells in the Temporomandibular Joint

    Kayoko Nozawa-Inoue, Nobuyuki Ikeda, Akiko Suzuki, Norio Amizuka, Takeyasu Maeda

    Journal of Oral Biosciences   46 ( 6 )   519 - 522   2004年

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    記述言語:英語  

    The synovial lining layer in the temporomandibular joint (TMJ) consists of macrophage-like type A cells and fibroblast-like type B cells. Their identification, however, has been difficult because of the lack of a specific cell marker. This review summarizes the characteristic configuration and development of the type B cell in the murine TMJ. Immunocytochemistry for 25kDa-heat shock protein (Hsp25) revealed two profiles of the fibroblast-like type B cells with cytoplasmic processes in the adult rat TMJ: one projected horizontally slender processes which covered the synovial membrane, and the other extended a thick, long process towards the articular cavity. The former appeared earlier than the latter during the development of synovial membrane, in close relation with the formation of the articular cavity and the commencement of active jaw movement. Since immature type B cells with Hsp25-immunoreactivity were found in the mesenchymal tissue which corresponded to the future articular cavity, type B cells may differentiate directly from mesenchymal cells. © 2004, Japanese Association for Oral Biology. All rights reserved.

    DOI: 10.2330/joralbiosci.46.519

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  • Synovial Type B Cells in the Temporomandibular Joint

    Kayoko Nozawa-Inoue, Nobuyuki Ikeda, Akiko Suzuki, Norio Amizuka, Takeyasu Maeda

    Journal of Oral Biosciences   46 ( 6 )   519 - 522   2004年

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    記述言語:英語  

    The synovial lining layer in the temporomandibular joint (TMJ) consists of macrophage-like type A cells and fibroblast-like type B cells. Their identification, however, has been difficult because of the lack of a specific cell marker. This review summarizes the characteristic configuration and development of the type B cell in the murine TMJ. Immunocytochemistry for 25kDa-heat shock protein (Hsp25) revealed two profiles of the fibroblast-like type B cells with cytoplasmic processes in the adult rat TMJ: one projected horizontally slender processes which covered the synovial membrane, and the other extended a thick, long process towards the articular cavity. The former appeared earlier than the latter during the development of synovial membrane, in close relation with the formation of the articular cavity and the commencement of active jaw movement. Since immature type B cells with Hsp25-immunoreactivity were found in the mesenchymal tissue which corresponded to the future articular cavity, type B cells may differentiate directly from mesenchymal cells. © 2004, Japanese Association for Oral Biology. All rights reserved.

    DOI: 10.2330/joralbiosci.46.519

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  • Morphological Approach to Biological Action of PTHrP and Vitamin D3 on Endochondral Ossification

    Norio Amizukam, Minqi Li, Kayoko Nozawa-Inoue, Yoshiro Kawano, Akiko Suzuki, Takeyasu Maeda, Kimimitsu Oda, Norio Amizuka, Janet E. Henderson, Kimimitsu Oda, Takeyasu Maeda, Janet E. Henderson, David Goltzman, John H. White, Andrew C. Karaplis

    Journal of Oral Biosciences   46 ( 2 )   79 - 96   2004年

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    記述言語:英語  

    Mice with a targeted deletion of parathyroid hormone (PTH) -related peptide (PTHrP) develop a form of dyschondroplasia resulting from diminished proliferation and precocious maturation of chondrocytes. The hypertrophic zone of the mutant epiphyseal cartilage revealed aberrant heterogeneous populations of chondrocytes, i.e., non-hypertrophic cells at different stages of differentiation. Therefore, PTHrP appears to play a central role in modulating cell proliferation and differentiation. The biological function of PTHrP is mediated by signaling pathway linked to the PTH/PTHrP receptor. However, the amino acids at 87–107 positions of PTHrP show a putative nucleolar targeting signal, and thought to involve in the resistance to apoptosis. Chondrocytic cell line, CFK2, transfected with truncated forms of PTHrP cDNA showed this peptide in the nucleoli mediated by translation initiating from AUG-codon and alternatively initiating from CUG codons. Thereby, PTHrP appears to act as a bipartite modulator of chondrocyte proliferation/differentiation, both through signal transduction linked to the PTH/PTHrP receptor and by its direct action in the nucleolus. Meanwhile, PTH/PTHrP receptor expression is controlled by two promoters in mouse, and the downstream promoter acts predominantly in bone and cartilage. We found 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] downregulated the activity of the downstream promoter in osteoblasts, but not in chondrocytes. This indicates that the interplay between PTH and 1,25(OH)2D3 is specific to their overlapping roles of serum calcium regulation in bone, and not to their complementary effects on proliferation/differentiation of chondrocytes. Thus, we will review our recent examinations on cartilage development in conjunction with the biological role in PTHrP, PTH/PTHrP receptor and 1,25(OH)2D3. © 2004, Japanese Association for Oral Biology. All rights reserved.

    DOI: 10.2330/joralbiosci.46.79

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  • Morphological Approach to Biological Action of PTHrP and Vitamin D3 on Endochondral Ossification

    Norio Amizukam, Minqi Li, Kayoko Nozawa-Inoue, Yoshiro Kawano, Akiko Suzuki, Takeyasu Maeda, Kimimitsu Oda, Norio Amizuka, Janet E. Henderson, Kimimitsu Oda, Takeyasu Maeda, Janet E. Henderson, David Goltzman, John H. White, Andrew C. Karaplis

    Journal of Oral Biosciences   46 ( 2 )   79 - 96   2004年

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    記述言語:英語  

    Mice with a targeted deletion of parathyroid hormone (PTH) -related peptide (PTHrP) develop a form of dyschondroplasia resulting from diminished proliferation and precocious maturation of chondrocytes. The hypertrophic zone of the mutant epiphyseal cartilage revealed aberrant heterogeneous populations of chondrocytes, i.e., non-hypertrophic cells at different stages of differentiation. Therefore, PTHrP appears to play a central role in modulating cell proliferation and differentiation. The biological function of PTHrP is mediated by signaling pathway linked to the PTH/PTHrP receptor. However, the amino acids at 87–107 positions of PTHrP show a putative nucleolar targeting signal, and thought to involve in the resistance to apoptosis. Chondrocytic cell line, CFK2, transfected with truncated forms of PTHrP cDNA showed this peptide in the nucleoli mediated by translation initiating from AUG-codon and alternatively initiating from CUG codons. Thereby, PTHrP appears to act as a bipartite modulator of chondrocyte proliferation/differentiation, both through signal transduction linked to the PTH/PTHrP receptor and by its direct action in the nucleolus. Meanwhile, PTH/PTHrP receptor expression is controlled by two promoters in mouse, and the downstream promoter acts predominantly in bone and cartilage. We found 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] downregulated the activity of the downstream promoter in osteoblasts, but not in chondrocytes. This indicates that the interplay between PTH and 1,25(OH)2D3 is specific to their overlapping roles of serum calcium regulation in bone, and not to their complementary effects on proliferation/differentiation of chondrocytes. Thus, we will review our recent examinations on cartilage development in conjunction with the biological role in PTHrP, PTH/PTHrP receptor and 1,25(OH)2D3. © 2004, Japanese Association for Oral Biology. All rights reserved.

    DOI: 10.2330/joralbiosci.46.79

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  • 骨代謝回転に応じた骨基質の微細構造変化と骨原性細胞の分化.日本骨粗鬆症学会雑誌

    Osteoporosis Japan   12(4) : 14-18   2004年

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  • Synovial membrane in the temporomandibular joint - Its morphology, function and development

    K Nozawa-Inoue, N Amizuka, N Ikeda, A Suzuki, Y Kawano, T Maeda

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   66 ( 4 )   289 - 306   2003年10月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    This paper reviews recent findings of the synovial membrane, in particular the morphology, function and development of synovial lining cells, in the temporomandibular joint (TMJ). Electron microscopic studies have confirmed the synovial membrane in TMJ consists of macrophage-like type A cells and fibroblast-like type B cells identical to those in other systematic joints. The macrophage-like type A cells react with anti-macrophage and macrophage-derived substances including the major histocompatibility class IT molecule, and show a drastic increase in their number in the inflamed synovial membrane. In addition, they have the ability to produce substances involved in the progression of TMJ inflammation such as nitric oxide and inducible nitric oxide synthase. Observation of osteopetrotic mice revealed that macrophage-like type A cells in TMJ are derived from monocyte lineage. Immunocytochemistry for 25kDa heat shock protein was able to depict the entire shape of fibro-blast-like type B cells including their unique processes. The expression of an estrogen receptor alpha-immunoreaction in the fibroblast-like type B cells may explain the etiology of temporomandibular disorders at a higher frequency in females than in males, suggesting that TMJ is a target tissue for estrogen. Furthermore, fibroblast-like type B cells are equipped with a basement membrane to serve as an adhesion molecule for the fibroblast-like type B cells to keep their epithelial arrangement. A clear understanding of the morphology of the intact synovial membrane will serve to clarify the etiology and development of temporomandibular disorders.

    DOI: 10.1679/aohc.66.289

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  • Regional differences in the distribution and type of immunocompetent cells in the rat oral mucosae

    A Suzuki, K Nozawa-Inoue, Y Kawano, N Amizuka, T Maeda

    BIOMEDICAL RESEARCH-TOKYO   24 ( 5 )   249 - 260   2003年10月

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    記述言語:英語   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    The distribution and type of immunocompetent cells were investigated in rat oral mucosae using immunocytochemistry and enzyme histochemistry, focusing on histological structures. We used two antibodies, OX6 and ED1, which recognize the rat la-antigen and macrophage/monocyte lineage, respectively. Enzymatic histochemistry for acid phosphatase (ACPase) activity and adenosine triphosphatase (ATPase) activity was also employed to identify macrophages and Langerhans cells, respectively. Many OX6-immunopositive cells, dendritic or irregular in shape, were recognizable in the lamina propria of oral mucosae: some cells extended their dendritic processes into the epithelial layer of the buccal and sublingual mucosae. Dendritic cells within the epithelium showed intense ATPase reaction, indicating they could be categorized as Langerhans cells. A small number of ED1-positive cells existed in the lamina propria, but none were present in the epithelial cell layer. Double staining either with OX6 and ED1 or OX6 and ACPase made it possible to divide the immunocompetent cells in the lamina propria into three types: the OX6-positive cells without ED1 or ACPase-reaction, the OX6-negative cells with ED1 and ACPase-reactions, and the OX6-ED1/ACPase-co-expressing cells, each of which possessed characteristic ultrastructural features demonstrated by immunoelectron microscopy. Taking these findings together with previous reports, these three types of cells were regarded as a dendritic-like cell, a macrophage without antigen-presentation ability, and a macrophage with antigen-presentation ability, respectively. There were regional differences in the distribution and density of these immunocompetent cells; they were densely distributed in order of the buccal and sublingual mucosae, the palatal mucosa, and the dorsal surface of tongue. The region-specific distribution and density of the immunocompetent cells might be due to the histological. structure of each oral mucosa, suggesting the presence of different immune-defense systems among each portion of the oral mucosae.

    DOI: 10.2220/biomedres.24.249

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  • Regional differences in the distribution and type of immunocompetent cells in the rat oral mucosae

    A Suzuki, K Nozawa-Inoue, Y Kawano, N Amizuka, T Maeda

    BIOMEDICAL RESEARCH-TOKYO   24 ( 5 )   249 - 260   2003年10月

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    記述言語:英語   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    The distribution and type of immunocompetent cells were investigated in rat oral mucosae using immunocytochemistry and enzyme histochemistry, focusing on histological structures. We used two antibodies, OX6 and ED1, which recognize the rat la-antigen and macrophage/monocyte lineage, respectively. Enzymatic histochemistry for acid phosphatase (ACPase) activity and adenosine triphosphatase (ATPase) activity was also employed to identify macrophages and Langerhans cells, respectively. Many OX6-immunopositive cells, dendritic or irregular in shape, were recognizable in the lamina propria of oral mucosae: some cells extended their dendritic processes into the epithelial layer of the buccal and sublingual mucosae. Dendritic cells within the epithelium showed intense ATPase reaction, indicating they could be categorized as Langerhans cells. A small number of ED1-positive cells existed in the lamina propria, but none were present in the epithelial cell layer. Double staining either with OX6 and ED1 or OX6 and ACPase made it possible to divide the immunocompetent cells in the lamina propria into three types: the OX6-positive cells without ED1 or ACPase-reaction, the OX6-negative cells with ED1 and ACPase-reactions, and the OX6-ED1/ACPase-co-expressing cells, each of which possessed characteristic ultrastructural features demonstrated by immunoelectron microscopy. Taking these findings together with previous reports, these three types of cells were regarded as a dendritic-like cell, a macrophage without antigen-presentation ability, and a macrophage with antigen-presentation ability, respectively. There were regional differences in the distribution and density of these immunocompetent cells; they were densely distributed in order of the buccal and sublingual mucosae, the palatal mucosa, and the dorsal surface of tongue. The region-specific distribution and density of the immunocompetent cells might be due to the histological. structure of each oral mucosa, suggesting the presence of different immune-defense systems among each portion of the oral mucosae.

    DOI: 10.2220/biomedres.24.249

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  • Synovial membrane in the temporomandibular joint - Its morphology, function and development

    K Nozawa-Inoue, N Amizuka, N Ikeda, A Suzuki, Y Kawano, T Maeda

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   66 ( 4 )   289 - 306   2003年10月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    This paper reviews recent findings of the synovial membrane, in particular the morphology, function and development of synovial lining cells, in the temporomandibular joint (TMJ). Electron microscopic studies have confirmed the synovial membrane in TMJ consists of macrophage-like type A cells and fibroblast-like type B cells identical to those in other systematic joints. The macrophage-like type A cells react with anti-macrophage and macrophage-derived substances including the major histocompatibility class IT molecule, and show a drastic increase in their number in the inflamed synovial membrane. In addition, they have the ability to produce substances involved in the progression of TMJ inflammation such as nitric oxide and inducible nitric oxide synthase. Observation of osteopetrotic mice revealed that macrophage-like type A cells in TMJ are derived from monocyte lineage. Immunocytochemistry for 25kDa heat shock protein was able to depict the entire shape of fibro-blast-like type B cells including their unique processes. The expression of an estrogen receptor alpha-immunoreaction in the fibroblast-like type B cells may explain the etiology of temporomandibular disorders at a higher frequency in females than in males, suggesting that TMJ is a target tissue for estrogen. Furthermore, fibroblast-like type B cells are equipped with a basement membrane to serve as an adhesion molecule for the fibroblast-like type B cells to keep their epithelial arrangement. A clear understanding of the morphology of the intact synovial membrane will serve to clarify the etiology and development of temporomandibular disorders.

    DOI: 10.1679/aohc.66.289

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  • The expression of estrogen receptor α (ERα) in the rat temporomandibular joint.

    Anat. Rec.   274A ( 2 )   934 - 941   2003年10月

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  • マウス下顎頭軟骨の経時的組織変化について

    HOSSAIN K S, 池田順行, 井上佳世子, 網塚憲生, 織田公光, 高木律男, 前田健康

    歯科基礎医学会雑誌   45 ( 5 )   304   2003年9月

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    記述言語:日本語  

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  • 口腔粘膜における抗原提示細胞の形態と分布

    鈴木 晶子, 野沢 佳世子[井上], 大島 勇人, 前田 健康

    歯科基礎医学会雑誌   45 ( 5 )   379 - 379   2003年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • ラット顎関節におけるMAPKAPK-2及びHsp25タンパクの局在

    野澤 佳世子, 井上, 河野 芳朗, 網塚 憲生, 前田 健康

    歯科基礎医学会雑誌   45 ( 5 )   303 - 303   2003年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 顎関節滑膜表層B型細胞の形態と機能

    池田 順行, 野澤 佳世子, 上, 高木 律男, 前田 健康

    歯界展望   102 ( 2 )   372 - 373   2003年8月

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    記述言語:日本語   出版者・発行元:医歯薬出版(株)  

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  • Temporal expression of immunoreactivity for heat shock protein 25 (Hsp25) in the rat periodontal ligament following transection of the inferior alveolar nerve

    K Iijima, F Harada, K Hanada, K Nozawa-Inoue, M Aita, Y Atsumi, S Wakisaka, T Maeda

    BRAIN RESEARCH   979 ( 1-2 )   146 - 152   2003年7月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    The present study examined the immunohistochemical localization of heat shock protein 25 (Hsp25) during the regeneration of nerve fibers and Schwann cells in the periodontal ligament of the rat lower incisor following transection of the inferior alveolar nerve. In the untreated control group, the periodontal ligament of rat incisor did not contain any Hsp25-immunoreaction. On postoperative day 3 (PO 3d), a small number of Schwann cells with slender cytoplasmic processes exhibited Hsp25-immunoreactivity. From PO 5d to PO 21d, Hsp25-positive nerve fibers and Schwann cells drastically increased in number in the alveolar half of the ligament. Although the axons of some regenerating Ruffini-like endings also showed Hsp25-immunoreactions, the migrated Schwann cells were devoid of Hsp25-immunoreaction. Thereafter, Hsp25-positive structures decreased in number gradually to disappear from the periodontal ligament by PO 56d. This temporal expression of Hsp25 in the periodontal ligament well-reflected the regeneration process of the nerve fibers. Hsp25 in the regenerating nerve fibers and denervated Schwann cells most likely serves in modulating actin dynamics and as a cellular inhibitor of apoptosis, respectively. (C) 2003 Elsevier B.V. All rights reserved.

    DOI: 10.1016/S0006-8993(03)02889-0

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  • Temporal expression of immunoreactivity for heat shock protein 25 (Hsp25) in the rat periodontal ligament following transection of the inferior alveolar nerve

    K Iijima, F Harada, K Hanada, K Nozawa-Inoue, M Aita, Y Atsumi, S Wakisaka, T Maeda

    BRAIN RESEARCH   979 ( 1-2 )   146 - 152   2003年7月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    The present study examined the immunohistochemical localization of heat shock protein 25 (Hsp25) during the regeneration of nerve fibers and Schwann cells in the periodontal ligament of the rat lower incisor following transection of the inferior alveolar nerve. In the untreated control group, the periodontal ligament of rat incisor did not contain any Hsp25-immunoreaction. On postoperative day 3 (PO 3d), a small number of Schwann cells with slender cytoplasmic processes exhibited Hsp25-immunoreactivity. From PO 5d to PO 21d, Hsp25-positive nerve fibers and Schwann cells drastically increased in number in the alveolar half of the ligament. Although the axons of some regenerating Ruffini-like endings also showed Hsp25-immunoreactions, the migrated Schwann cells were devoid of Hsp25-immunoreaction. Thereafter, Hsp25-positive structures decreased in number gradually to disappear from the periodontal ligament by PO 56d. This temporal expression of Hsp25 in the periodontal ligament well-reflected the regeneration process of the nerve fibers. Hsp25 in the regenerating nerve fibers and denervated Schwann cells most likely serves in modulating actin dynamics and as a cellular inhibitor of apoptosis, respectively. (C) 2003 Elsevier B.V. All rights reserved.

    DOI: 10.1016/S0006-8993(03)02889-0

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  • Immunocytochemical demonstration of MAPKAPK-2 and Hsp25 in the rat temporomandibular joint

    K. Nozawa-Inoue, Y. Kawano, N. Amizuka, T. Maeda

    JOURNAL OF DENTAL RESEARCH   82   B160 - B160   2003年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • ラット顎関節におけるB型細胞の出現

    池田 順行, 野沢 佳世子, 上, 高木 律男, 前田 健康

    日本顎関節学会雑誌   15 ( 1 )   102 - 102   2003年4月

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    記述言語:日本語   出版者・発行元:(一社)日本顎関節学会  

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  • Immunohistochemical localization of periostin in developing long bones of mice

    Y Hirose, H Suzuki, N Amizuka, J Shimomura, Y Kawano, K Nozawa-Inoue, A Kudo, T Maeda

    BIOMEDICAL RESEARCH-TOKYO   24 ( 1 )   31 - 37   2003年2月

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    記述言語:英語   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    The immunolocalization of periostin, previously termed osteoblast-specific factor 2, was investigated in developing long bones of 17-day-old fetal mice and of 1-, 2-, 3- and 8-week-old mice at the light and electron microscopic levels. Fetal femurs showed immunoreactions for periostin in the periosteum, perichondrium, articular surface of the epiphyseal cartilage, joint ligaments, and fascias of surrounding muscles. In particular, intense immunoreactivity for periostin was found in the fibrous layer of the periosteum and perichondrium. At postnatal 1-and 2-weeks, in contrast, the immunoreactivity was restricted to the periosteum and thick fascias of surrounding muscles when compared with the fetal bones. Immunoelectron microscopic observation of the periosteum demonstrated immunoreaction products for periostin at the junction of periosteal fibroblasts and collagen bundles, suggesting its competence in the cell-to-matrix interaction. Mice at 3 and 8 weeks, unlike 2-week-old mice, showed a periostin-immunoreaction dominantly in the osteoblastic layer but not in the fibroblastic layer of the periosteum. Furthermore, the perichondrium and fascias of surrounding muscles were devoid of immunoreaction. Thus, periostin was confirmed to be widely distributed in bone and concomitant tissues at the fetal stage. As mice grew, however, its immunoreactivity gradually came to be restricted to the osteoblastic layer of the periosteum. Our findings suggest that periostin acts at the site of the cell-to-matrix interaction in periosteum, fascias, and joint ligament during morphogenesis of these tissues.

    DOI: 10.2220/biomedres.24.31

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  • Immunohistochemical localization of periostin in developing long bones of mice

    Y Hirose, H Suzuki, N Amizuka, J Shimomura, Y Kawano, K Nozawa-Inoue, A Kudo, T Maeda

    BIOMEDICAL RESEARCH-TOKYO   24 ( 1 )   31 - 37   2003年2月

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    記述言語:英語   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    The immunolocalization of periostin, previously termed osteoblast-specific factor 2, was investigated in developing long bones of 17-day-old fetal mice and of 1-, 2-, 3- and 8-week-old mice at the light and electron microscopic levels. Fetal femurs showed immunoreactions for periostin in the periosteum, perichondrium, articular surface of the epiphyseal cartilage, joint ligaments, and fascias of surrounding muscles. In particular, intense immunoreactivity for periostin was found in the fibrous layer of the periosteum and perichondrium. At postnatal 1-and 2-weeks, in contrast, the immunoreactivity was restricted to the periosteum and thick fascias of surrounding muscles when compared with the fetal bones. Immunoelectron microscopic observation of the periosteum demonstrated immunoreaction products for periostin at the junction of periosteal fibroblasts and collagen bundles, suggesting its competence in the cell-to-matrix interaction. Mice at 3 and 8 weeks, unlike 2-week-old mice, showed a periostin-immunoreaction dominantly in the osteoblastic layer but not in the fibroblastic layer of the periosteum. Furthermore, the perichondrium and fascias of surrounding muscles were devoid of immunoreaction. Thus, periostin was confirmed to be widely distributed in bone and concomitant tissues at the fetal stage. As mice grew, however, its immunoreactivity gradually came to be restricted to the osteoblastic layer of the periosteum. Our findings suggest that periostin acts at the site of the cell-to-matrix interaction in periosteum, fascias, and joint ligament during morphogenesis of these tissues.

    DOI: 10.2220/biomedres.24.31

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  • The expression of estrogen receptor α (ERα) in the rat temporomandibular joint.

    Anat. Rec.   274A, 934-941   2003年

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  • Convergence of selected inputs premotor neurons with possible from sensory afferents to trigeminal projections to masseter motoneurons in the rabbit

    M Inoue, K Nozawa-Inoue, R Donga, Y Yamada

    BRAIN RESEARCH   957 ( 1 )   183 - 191   2002年12月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Peripheral input convergence on trigeminal premotor neurons in the vicinity of trigeminal motor nucleus has been investigated. Thirty neurons were identified by their antidromic responses to microstimulation of the masseteric subnucleus of trigeminal motor nucleus (NVmot-mass). Peripheral receptive fields were found in the buccal mucosae, periodontal ligaments, palate, tongue and vibrissae for 16 neurons located in the intertrigeminal area (NVint), supratrigeminal area (NVs), main sensory trigeminal nucleus (NVsnpr) and subnucleus gamma of the oral nucleus of the spinal trigeminal tract (NVspo-gamma). Eleven neurons in the NVint, NVs and NVspo-gamma responded to passive jaw opening: nine neurons were activated and two were inhibited. None of the neurons responded to both the orofacial mechanical stimulation and passive jaw opening. Forty-six percent of neurons (13 out of 28 tested) received inputs from the inferior alveolar nerve (IAN) and 53% of neurons (8 out of 15 tested) received inputs from the infraorbital nerve (ION). Out of 15 neurons tested for inputs from the IAN and ION, 7 neurons in the NVsnpr and NVspo-gamma received input from both. Sixteen percent of neurons (4 out of 25) received inputs from the masseteric nerve (MassN). None of the neurons with inputs from IAN and/or ION also received inputs from the MassN. We suggest that trigeminal premotor interneurons with projections to the NVmot-mass fall into two broad categories, those with inputs from the IAN and/or ION and those with inputs from the MassN, possibly muscle spindle afferents, and no neuron receiving inputs from both. (C) 2002 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0006-8993(02)03662-4

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  • Convergence of selected inputs premotor neurons with possible from sensory afferents to trigeminal projections to masseter motoneurons in the rabbit

    M Inoue, K Nozawa-Inoue, R Donga, Y Yamada

    BRAIN RESEARCH   957 ( 1 )   183 - 191   2002年12月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Peripheral input convergence on trigeminal premotor neurons in the vicinity of trigeminal motor nucleus has been investigated. Thirty neurons were identified by their antidromic responses to microstimulation of the masseteric subnucleus of trigeminal motor nucleus (NVmot-mass). Peripheral receptive fields were found in the buccal mucosae, periodontal ligaments, palate, tongue and vibrissae for 16 neurons located in the intertrigeminal area (NVint), supratrigeminal area (NVs), main sensory trigeminal nucleus (NVsnpr) and subnucleus gamma of the oral nucleus of the spinal trigeminal tract (NVspo-gamma). Eleven neurons in the NVint, NVs and NVspo-gamma responded to passive jaw opening: nine neurons were activated and two were inhibited. None of the neurons responded to both the orofacial mechanical stimulation and passive jaw opening. Forty-six percent of neurons (13 out of 28 tested) received inputs from the inferior alveolar nerve (IAN) and 53% of neurons (8 out of 15 tested) received inputs from the infraorbital nerve (ION). Out of 15 neurons tested for inputs from the IAN and ION, 7 neurons in the NVsnpr and NVspo-gamma received input from both. Sixteen percent of neurons (4 out of 25) received inputs from the masseteric nerve (MassN). None of the neurons with inputs from IAN and/or ION also received inputs from the MassN. We suggest that trigeminal premotor interneurons with projections to the NVmot-mass fall into two broad categories, those with inputs from the IAN and/or ION and those with inputs from the MassN, possibly muscle spindle afferents, and no neuron receiving inputs from both. (C) 2002 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0006-8993(02)03662-4

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  • ラット顎関節におけるエストロゲンレセプターαの免疫組織化学的研究

    山田 一穂, 河野 正司, 野澤 佳世子, 網塚 憲生, 前田 健康

    新潟歯学会雑誌   32 ( 2 )   353 - 353   2002年12月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • ラット顎関節滑膜B型細胞の発生過程

    池田順行, 井上佳世子, 高木律男, 前田健康

    歯科基礎医学会雑誌   44 ( 5 )   452   2002年10月

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    記述言語:日本語  

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  • ラット顎関節におけるエストロゲン受容体αの微細構造学的局在について

    山田 一穂, 河野 正司, 井上-野澤, 佳世子, 網塚 憲生, 前田 健康

    歯科基礎医学会雑誌   44 ( 5 )   408 - 408   2002年9月

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    記述言語:日本語   出版者・発行元:歯科基礎医学会  

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  • The expression of copper, zinc superoxide dismutase (Cu/Zn-SOD) in the rat submandibular gland

    H Yamamoto, Y Kawano, K Nozawa-Inoue, M Aita, Y Miki, J Sasaki, T Maeda

    BIOMEDICAL RESEARCH-TOKYO   23 ( 2 )   85 - 90   2002年4月

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    記述言語:英語   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    Copper-zinc superoxide dismutase (Cu/Zn-SOD), a scavenger and resolving enzyme of superoxide, an active oxygen species, has the potential to induce aging and various diseases. The present study examined the expression of Cu/Zn-SOD mRNA and the localization of Cu/Zn-SOD protein in the adult rat submandibular gland by in situ hybridization and immunohistochemistry. Both signals for Cu/Zn-SOD mRNA and immunoreactivity were recognizable in all types of ducts including intercalated, striated, granular and excretory ducts, but never in acini. The Cu/Zn-SOD-expressing cells intermingled with some duct cells that had neither a Cu/Zn-SOD mRNA expression nor immunoreaction for Cu/Zn-SOD. These findings suggest that the duct cells in the rat submandibular gland exhibit greater resistance than the acinar cells against oxidative stress.

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  • The expression of copper, zinc superoxide dismutase (Cu/Zn-SOD) in the rat submandibular gland

    H Yamamoto, Y Kawano, K Nozawa-Inoue, M Aita, Y Miki, J Sasaki, T Maeda

    BIOMEDICAL RESEARCH-TOKYO   23 ( 2 )   85 - 90   2002年4月

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    記述言語:英語   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    Copper-zinc superoxide dismutase (Cu/Zn-SOD), a scavenger and resolving enzyme of superoxide, an active oxygen species, has the potential to induce aging and various diseases. The present study examined the expression of Cu/Zn-SOD mRNA and the localization of Cu/Zn-SOD protein in the adult rat submandibular gland by in situ hybridization and immunohistochemistry. Both signals for Cu/Zn-SOD mRNA and immunoreactivity were recognizable in all types of ducts including intercalated, striated, granular and excretory ducts, but never in acini. The Cu/Zn-SOD-expressing cells intermingled with some duct cells that had neither a Cu/Zn-SOD mRNA expression nor immunoreaction for Cu/Zn-SOD. These findings suggest that the duct cells in the rat submandibular gland exhibit greater resistance than the acinar cells against oxidative stress.

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  • Changes in jaw reflexes by stimulation of the hypothalamus in anesthetized rabbits

    M Inoue, K Nozawa-Inoue, Y Miyaoka, Y Yamada

    NEUROSCIENCE RESEARCH   41 ( 1 )   61 - 65   2001年9月

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    記述言語:英語   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    Changes in the masseteric monosynaptic reflex (MMR) and jaw-opening reflex (JOR) responses resulting from conditioning stimulation in the hypothalamus were studied in anesthetized rabbits. Stimulation of the lateral hypothalamus evoked a facilitation of the MMR and an inhibitory or facilitatory effect on the JOR. The facilitatory effect on JOR was stronger than that on the MMR. The facilitatory effective site for the JOR was in the dorsal and lateral directions as compared to the inhibitory field. The results suggest two functionally distinct regions in the lateral hypothalamus that separately project to the jaw-opening muscles. (C) 2001 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/S0168-0102(01)00260-7

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  • Synovial type B cells express 25kDa heat shock protein (Hsp25) in mouse temporomandibular joint. (Jointly authored)

    Arch. Oral Biol.   46(10), 947-954   2001年

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  • Synovial type B cells express 25kDa heat shock protein (Hsp25) in mouse temporomandibular joint. (Jointly authored)

    Arch. Oral Biol.   46(10), 947-954   2001年

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  • Immunocytochemical detection of superoxide dismutases (SODs) in the periodontal Ruffini endings of the rat incisor. (Jointly authored)

    Brain Res.   905(1-2), 232-235   2001年

  • Immunocytochemical detection of superoxide dismutases (SODs) in the periodontal Ruffini endings of the rat incisor. (Jointly authored)

    Brain Res.   905(1-2), 232-235   2001年

  • Changes in jaw reflexes by stimulation of the hypothalamus in anesthetized rabbits. (Jointly authored)

    Neurosci. Res.   41(1), 61-5   2001年

  • Transient expression of heat shock protein (Hsp) 25 in the dental pulp and enamel organ during odontogenesis in the rat incisor

    H Ohshima, H Ajima, Y Kawano, K Nozawa-Inoue, S Wakisaka, T Maeda

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   63 ( 4 )   381 - 395   2000年10月

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    記述言語:英語   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    The expression of heat shock protein (Hsp) 25 during odontogenesis in the dental pulp and enamel organ of rat incisors was investigated by immunocytochemistry and confocal microscopy. In the process of dentin formation, immature odontoblasts first exhibited Hsp 25-immunoreactivity, and increased in immunointensity with the advance of their differentiation. In the dental pulp, in contrast, intense immunoreaction in the mesenchymal cells became weak or negative in parallel with the progress of cell differentiation. The immunoreaction for Hsp 25 in the enamel organ revealed a characteristic stage-related alteration during amelogenesis. In secretory ameloblasts, the immunoreaction for Hsp 25 was found throughout their cell bodies, intense reactivity being located near the proximal and distal terminal webs. At the maturation stage, ruffle-ended ameloblasts (RA) consistently showed Hsp 25-immunoreactivity throughout the cell bodies, whereas smooth-ended ameloblasts (SA) lacking a ruffled border were weak in immunoreaction at the distal cytoplasm. Other cellular elements of the enamel organ were negative. The subcellular localization of Hsp 25-immunoreactivity in this study appeared essentially identical to that of actin filaments as demonstrated by confocal microscopy using rhodamine-labeled phalloidin. These immunocytochemical data suggest that the Hsp 25 molecule is involved in reinforcement of the cell layer following cell movement during odontogenesis and in the formation and maintenance of the ruffled border of RA.

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  • 顎関節滑膜の形態

    野澤 佳世子, 井上, 高木 律男, 前田 健康

    クイントエッセンス   18(9), 3-14 ( 10 )   1965 - 1976   2000年10月

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  • 顎関節におけるHSP 25/27の分布に関する免疫細胞化学的研究

    井上 佳世子, 野澤, 高木 律男, 前田 健康

    日本顎関節学会雑誌   12 ( 1 )   177 - 177   2000年4月

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  • Morphology and functional roles of synoviocytes in the joint

    T Iwanaga, M Shikichi, H Kitamura, H Yanase, K Nozawa-Inoue

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   63 ( 1 )   17 - 31   2000年3月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    The joint capsule exhibits a unique cellular lining in the luminal surface of the synovial membrane. The synovial intimal cells, termed synoviocytes, are believed to be responsible for the production of synovial fluid components, for absorption from the joint cavity, and for blood/synovial fluid exchanges, but their detailed structure and function as well as pathological changes remain unclear. Two types of synoviocytes, macrophagic cells (type A cells) and fibroblast-like cells (type B cells) have been identified. Type A synoviocytes are non-fixed cells that can phagocytose actively cell debris and wastes in the joint cavity, and possess an antigen-presenting ability. These type a cells, derived from blood-borne mononuclear cells, can be considered resident macrophages (tissue macrophages) like hepatic Kupffer cells. Type B synoviocytes are characterized by the rich existence of rough endoplasmic reticulum, and dendritic processes which form a regular network in the luminal surface of the synovial membrane. Their complex three-dimensional architecture was first revealed by our recent scanning electron microscopy of macerated samples. The type B cells, which are proper synoviocytes, are involved in production of specialized matrix constituents including hyaluronan, collagens and fibronectin for the intimal interstitium and synovial fluid. The proliferative potentials of type B cells in loco are much higher than type B cells, although the transformation of subintimal fibroblasts into type B cells can not be excluded. In some mammals, type B cells show features suggesting endocrine and sensory functions, but these are not recognized in other species. The synoviocytes, which form a discontinuous cell layer, develop both fragmented basement membranes around the cells and junctional apparatus such as desmosomes and gap junctions. For an exact understanding of the mechanism of arthritis, we need to establish the morphological background of synoviocytes as well as their functions under normal conditions.

    DOI: 10.1679/aohc.63.17

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  • Morphology and functional roles of synoviocytes in the joint

    T Iwanaga, M Shikichi, H Kitamura, H Yanase, K Nozawa-Inoue

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   63 ( 1 )   17 - 31   2000年3月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    The joint capsule exhibits a unique cellular lining in the luminal surface of the synovial membrane. The synovial intimal cells, termed synoviocytes, are believed to be responsible for the production of synovial fluid components, for absorption from the joint cavity, and for blood/synovial fluid exchanges, but their detailed structure and function as well as pathological changes remain unclear. Two types of synoviocytes, macrophagic cells (type A cells) and fibroblast-like cells (type B cells) have been identified. Type A synoviocytes are non-fixed cells that can phagocytose actively cell debris and wastes in the joint cavity, and possess an antigen-presenting ability. These type a cells, derived from blood-borne mononuclear cells, can be considered resident macrophages (tissue macrophages) like hepatic Kupffer cells. Type B synoviocytes are characterized by the rich existence of rough endoplasmic reticulum, and dendritic processes which form a regular network in the luminal surface of the synovial membrane. Their complex three-dimensional architecture was first revealed by our recent scanning electron microscopy of macerated samples. The type B cells, which are proper synoviocytes, are involved in production of specialized matrix constituents including hyaluronan, collagens and fibronectin for the intimal interstitium and synovial fluid. The proliferative potentials of type B cells in loco are much higher than type B cells, although the transformation of subintimal fibroblasts into type B cells can not be excluded. In some mammals, type B cells show features suggesting endocrine and sensory functions, but these are not recognized in other species. The synoviocytes, which form a discontinuous cell layer, develop both fragmented basement membranes around the cells and junctional apparatus such as desmosomes and gap junctions. For an exact understanding of the mechanism of arthritis, we need to establish the morphological background of synoviocytes as well as their functions under normal conditions.

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  • Transient expression of heat shock protein (Hsp) 25 in the dental pulp and enamel organ during odontogenesis in the rat incisor. (Jointly authored)

    Arch. Histol. Cytol.   63(4), 381-395   2000年

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  • Immunocytochemical demonstration of heat shock protein 25 in the rat temporomandibular joint

    K Nozawa-Inoue, H Ohshima, Y Kawano, H Yamamoto, R Takagi, T Maeda

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   62 ( 5 )   483 - 491   1999年12月

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    記述言語:英語   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    The expression of heat shock protein 25 (Hsp 25) was investigated in the rat temporomandibular joint by immunocytochemistry combined with confocal and electron microscopy. Immunostaining with an antibody to Hsp25 was able to demonstrate various cellular elements in the synovial membrane of the joint. Intense immunoreaction for Hsp25 was recognized in certain cells comprising the synovial lining layer. Confocal microscopic observation revealed two characteristic profiles of the Hsp25-positive cells with cytoplasmic processes: one extended thick and long processes towards the articular cavity, and the other prejected horizontally slender processes which covered the synovial membrane. Under the electron microscope, the immunoreactive synovial lining cells were characterized by a well-developed rough endoplasmic reticulum and secretory granules, suggesting that they can be categorized as fibroblastic type B cells. The covering by the cytoplasmic extensions was confirmed by immune-electron microscopic observations. This cytoplasmic covering presumably performs a barrier function and expedites the effective secretion/resorption of synovial fluids. Since it has been proposed that Hsp 25 is associated with an estrogen receptor, the immunopositive synovial lining cells were considered estrogen-target cells. Immunoreactivity for Hsp25 was also observed in the chondrocytes of the maturative and hypertrophic cell layers as well as in the cells of the articular disk. A suggestion was made that Hsp 25 might be involved in the inhibition of apoptosis of those cells.

    DOI: 10.1679/aohc.62.483

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  • 顎関節滑膜組織の観察 顎関節症状を有さない悪性腫瘍患者について

    小林 龍彰, 安島 久雄, 高木 律男, 野澤 佳世子, 前田 健康

    日本口腔外科学会雑誌   45 ( 13 )   1020 - 1020   1999年12月

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    記述言語:日本語   出版者・発行元:(公社)日本口腔外科学会  

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  • ラット切歯歯髄・エナメル器における低分子熱ショック蛋白の発現について

    大島 勇人, 安島 久雄, 井上 佳世子, 河野 芳朗, 脇坂 聡, 前田 健康

    歯科基礎医学会雑誌   41 ( 5 )   436 - 436   1999年8月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • Immunocytochemical demonstration of laminin in the synovial lining layer of the rat temporomandibular joint

    K Nozawa-Inoue, H Ajima, R Takagi, T Maeda

    ARCHIVES OF ORAL BIOLOGY   44 ( 6 )   531 - 534   1999年6月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    This immunocytochemical study describes the distribution of laminin in the synovial lining of the rat temporomandibular joint. Laminin immunostaining was present around some synovial lining cells and blood vessels. Ultrastructurally, immunoreactive products for laminin were deposited around cells with a well-developed rough endoplasmic reticulum and secretory granules, suggesting that they were type B synovial lining cells. The localization of laminin immunoreactivity was not uniform around the cell membrane, the most intense immunoreaction being present on the basal aspect membrane as is seen in the basement membrane of epithelia. In contrast, macrophage-like synovial lining type A cells did not show laminin immunoreactivity. This different immunostaining pattern suggests that laminin acts as an adhesion molecule for the type B cells in their epitheliallike arrangement. (C) 1999 Elsevier Science Ltd. All rights reserved.

    DOI: 10.1016/S0003-9969(99)00021-7

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  • Immunocytochemical demonstration of laminin in the synovial lining layer of the rat temporomandibular joint

    K. Nozawa-Inoue, H. Ajima, R. Takagi, T. Maeda

    Archives of Oral Biology   44 ( 6 )   531 - 534   1999年6月

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    記述言語:英語  

    This immunocytochemical study describes the distribution of laminin in the synovial lining of the rat temporomandibular joint. Laminin immunostaining was present around some synovial lining cells and blood vessels. Ultrastructurally, immunoreactive products for laminin were deposited around cells with a well-developed rough endoplasmic reticulum and secretory granules, suggesting that they were type B synovial lining cells. The localization of laminin immunoreactivity was not uniform around the cell membrane, the most intense immunoreaction being present on the basal aspect membrane as is seen in the basement membrane of epithelia. In contrast, macrophage-like synovial lining type A cells did not show laminin immunoreactivity. This different immunostaining pattern suggests that laminin acts as an adhesion molecule for the type B cells in their epithelial-like arrangement.

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  • ラット顎関節滑膜に基底膜が存在する

    野澤 佳世子, 前田 健康, 高木 律男, 大橋 靖

    日本顎関節学会雑誌   11 ( 1 )   106 - 106   1999年4月

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    記述言語:日本語   出版者・発行元:(一社)日本顎関節学会  

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  • 当科において最近6年間に経験した顎関節症患者の臨床統計的観察

    井上 達夫, 高木 律男, 小林 龍彰, 野澤 佳世子, 西原 義之, 安島 久雄, 大橋 靖

    日本顎関節学会雑誌   11 ( 1 )   75 - 75   1999年4月

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  • Immunohistochemical localization of S-100β in the dental pulp of the rat molar

    Y. Atsumi, K. Nozawa-Inoue, T. Maeda, K. Kurisu, S. Wakisaka

    Brain Research   818 ( 2 )   515 - 519   1999年2月

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    記述言語:英語  

    The present study was undertaken to reveal whether S-100α or S-100β or both are present in the nerve fibers in the rat molar tooth pulp. No immunoreactivity for S-100α was observed in the molar pulp. In the root pulp, thick smooth-surfaced structures accompanying the blood vessel showed S-100β-like immunoreactivity (-LI), and occasionally a very few thin beaded elements exhibited S-100β-LI. In the coronal pulp, S-100β-like immunoreactive (-IR) structures arborized repeatedly and extensively
    they had a predominantly thick, smooth-surfaced appearance, though parts appeared thin and beaded. Numerous thin varicose S-100β-IR structures ran through the odontoblast cell layer, and further penetrated into the predentin alongside the dentinal tubules. They could be traced for approximately 10-20 μm into the predentin from the pulp-predentin border. Immunoelectron microscopy revealed that the Schwann cells in the root pulp showed S-100β-LI, and that S-100β-LI was present in the axoplasm as well as Schwann cells in the coronal pulp. The S-100β-IR axons were rarely surrounded by S-100β-IR Schwann cells. In the predentin, S-100β-IR nerve fibers terminated in a position close to the odontoblast processes. The present findings indicate that S-100β, not S-100α, is present in the axon in the dental pulp and predentin as well as in the Schwann cells.

    DOI: 10.1016/S0006-8993(98)01221-9

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  • Immunohistochemical localization of S-100β in the dental pulp of the rat molars. "jointly authored"

    ATSUMI Y, NOZAWA‐INOUE K, MAEDA T, KURISU K, WAKISAKA S

    Brain Research (   818 ( 2 )   515 - 519   1999年2月

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  • Imunocytochemical demonstration of heat shock protein 25 in the rat temporomandibular joint.

    Arch. Histol. Cytol.   62(5), 483-491   1999年

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  • 下顎頭に発生した骨軟骨腫の1例

    新潟歯学会雑誌   29(1), 29-32   1999年

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  • Immunocytochemical detection of S-100β in the periodontal Ruffini endings in the rat incisor

    Kuniko Nakakura-Ohshima, Sachiko Hayashi, Yukako Atsumi, Satoshi Wakisaka, Kayoko Nozawa-Inoue, Takeyasu Maeda

    Neuroscience Letters   258 ( 3 )   163 - 166   1998年12月

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    記述言語:英語  

    Subcellular localization of S-100 protein, a kind of calcium binding proteins, was examined immunohistochemically in the Ruffini ending, a primary mechanoreceptor, in the periodontal ligament of the rat incisor. The periodontal ligament of the rat incisor was found to contain many S-100β- immunoreactive (-IR) structures but no S-100α-IR elements. The S-100β-IR structures ramified extensively to form Ruffini endings and were frequently associated with round cells, the terminal Schwann cells, which also showed S- 100β-like immunoreactivity. In many periodontal Ruffini endings, S-100β-IR products were recognized in the cytoplasm of Schwann cells, but not in the axoplasm. However, some axon terminals which had fewer or shorter axonal fingers, were filled with S-100β-IR products. The present findings indicated the existence of S-100β, not S-100α, in axon terminals of the periodontal mechanoreceptive endings which were identified as type II Ruffini endings.

    DOI: 10.1016/S0304-3940(98)00872-6

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  • Intermittent Closed Lockに対するIVROの臨床的検討

    小林 龍彰, 野澤 佳世子, 福田 全孝, 井上 達夫, 西原 義之, 安島 久雄, 高木 律男, 大橋 靖

    日本口腔科学会雑誌   47 ( 5 )   604 - 604   1998年12月

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    記述言語:日本語   出版者・発行元:(NPO)日本口腔科学会  

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  • Immunocytochemical demonstration of the synovial membrane in experimentally induced arthritis of the rat temporomandibular joint

    K Nozawa-Inoue, R Takagi, T Kobayashi, Y Ohashi, T Maeda

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   61 ( 5 )   451 - 466   1998年12月

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    記述言語:英語   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    The present study is first to report an experimental model of adjuvant-induced arthritis in the rat temporomandibular joint (TMJ). Arthritis was induced by simultaneous intradermal administrations of Freund's complete adjuvant, one at the parietal scalp and the other at the base of the tail. In this model, we demonstrated responses of the synovial membrane by immunocytochemistry using antibodies to OX6 and ED1 which recognize Ia antigen in MHC class II antigen-expressing cells and the macrophage/monocyte lineage, respectively. Three weeks after administration, no remarkable signs of inflammation were macroscopically recognizable in the TMJ, but microscopically the synovial membrane in the TMJ revealed marked changes such as enhanced vascularization and hemostasis in the sublining layer and a thickening in the synovial lining cell layer. Intense OX6-immunoreactivity was found in the synovial lining cells at lesions in the experimental group but not in the control group. Immunoelectron microscopy revealed that these OX6-immunopositive synovial lining cells developed dense cytoplasmic processes and numerous vacuoles and vesicles, resembling type A cells. Part of the type A cells also showed ED1-immunoreactivity. The expression of OX6 or ED1 immunoreactivity in the synovial lining cells might be involved in the initial immune responses in this arthritis model because the synovial membranes are exposed to the synovial fluids which have been believed to contain antigenic substances.

    DOI: 10.1679/aohc.61.451

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  • Immunocytochemical demonstration of the synovial membrane in experimentally induced arthritis of the rat temporomandibular joint

    K Nozawa-Inoue, R Takagi, T Kobayashi, Y Ohashi, T Maeda

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   61 ( 5 )   451 - 466   1998年12月

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    記述言語:英語   出版者・発行元:INT SOC HISTOLOGY & CYTOLOGY  

    The present study is first to report an experimental model of adjuvant-induced arthritis in the rat temporomandibular joint (TMJ). Arthritis was induced by simultaneous intradermal administrations of Freund's complete adjuvant, one at the parietal scalp and the other at the base of the tail. In this model, we demonstrated responses of the synovial membrane by immunocytochemistry using antibodies to OX6 and ED1 which recognize Ia antigen in MHC class II antigen-expressing cells and the macrophage/monocyte lineage, respectively. Three weeks after administration, no remarkable signs of inflammation were macroscopically recognizable in the TMJ, but microscopically the synovial membrane in the TMJ revealed marked changes such as enhanced vascularization and hemostasis in the sublining layer and a thickening in the synovial lining cell layer. Intense OX6-immunoreactivity was found in the synovial lining cells at lesions in the experimental group but not in the control group. Immunoelectron microscopy revealed that these OX6-immunopositive synovial lining cells developed dense cytoplasmic processes and numerous vacuoles and vesicles, resembling type A cells. Part of the type A cells also showed ED1-immunoreactivity. The expression of OX6 or ED1 immunoreactivity in the synovial lining cells might be involved in the initial immune responses in this arthritis model because the synovial membranes are exposed to the synovial fluids which have been believed to contain antigenic substances.

    DOI: 10.1679/aohc.61.451

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  • Immunocytochemical detection of S-100β in the periodontal Ruffini endings in the rat incisor. "jointly authored"

    NAKAKURA‐OHSHIMA K, HAYASHI S, ATSUMI Y, WAKISAKA S, NOZAWA‐INOUE K, MAEDA T

    Neuroscience Letters   258 ( 3 )   163 - 166   1998年12月

  • ヒト下顎位置感覚への顎関節受容器および咀嚼筋受容器の関与

    小野 和宏, 高木 律男, 小林 龍彰, 野澤 佳世子, 大橋 靖, 田口 洋

    日本口腔科学会雑誌   47 ( 2 )   199 - 205   1998年

  • 顎関節症患者に対するヒアルロン酸ナトリウム注入療法の評価

    新潟歯学会雑誌   27 ( 2 )   153 - 159   1997年

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  • 下唇粘膜部に生じた粘表皮癌の1例

    野澤 佳世子, 高木 律男, 小野 和宏, 大橋 靖, 入江 太朗, 棟方 隆一

    日本口腔外科学会雑誌   43 ( 4 )   355 - 357   1997年

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受賞

  • 歯科基礎医学会賞

    2005年  

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    受賞国:日本国

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  • Rising Members Award of Japanese Association for Oral Biology

    2005年  

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共同研究・競争的資金等の研究

  • ヒト顎関節オルガノイド作製への挑戦

    2015年4月 - 2017年3月

    日本学術振興会  科学研究費補助金(挑戦的萌芽) 

    井上 佳世子

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    担当区分:研究代表者  資金種別:競争的資金

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  • 顎関節滑膜表層細胞におけるデスミン陽性B型細胞の血管新生への関与

    2012年4月 - 2015年3月

    日本学術振興会  科学研究費補助金(基盤(C)) 

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    担当区分:研究代表者  資金種別:競争的資金

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  • 顎関節滑膜における線維芽細胞様B型細胞の分類確立~筋特異的分子に着目して~

    2009年4月 - 2012年3月

    文部科学省  科学研究費補助金(若手研究(B)) 

    井上 佳世子

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    担当区分:研究代表者  資金種別:競争的資金

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  • 顎関節滑膜表層細胞におけるカベオラの存在意義

    2006年4月 - 2009年3月

    文部科学省  科学研究費補助金(若手研究(B)) 

    井上 佳世子

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    担当区分:研究代表者  資金種別:競争的資金

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  • 顎関節滑膜表層細胞におけるp38ストレス応答経路

    2004年4月 - 2006年3月

    文部科学省  科学研究費補助金(若手研究(B)) 

    井上 佳世子

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    担当区分:研究代表者  資金種別:競争的資金

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  • エストロゲン標的器官としての顎関節滑膜表層細胞―免疫細胞化学的,分子生物学的研究―

    2002年4月 - 2004年3月

    文部科学省  科学研究費補助金(若手研究(B)) 

    井上 佳世子

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    担当区分:研究代表者  資金種別:競争的資金

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  • Immunocytochemical study of the synovial lining cells in the temporomandibular joint

    1994年

    Grant-in-Aid for Scientific Research 

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