Graduate School of Science and Technology Life and Food Sciences Life Sciences Professor
Faculty of Science Department of Science Professor
Updated on 2024/03/29
理学博士 ( 1987.12 北海道大学 )
Developmental Biology
発生生物学
Life Science / Developmental biology
Niigata University Graduate School of Science and Technology Professor
2012
Niigata University Faculty of Science Associate Professor
2007 - 2012
Associate Prof, Niigata University, Faculty
1994
Niigata University Faculty of Science Associate Professor (as old post name)
1994 - 2007
米国立がん研究所 フレデリックリサーチセンター 研究員
1990 - 1994
Visiting fellow, NIH, USA
1990 - 1994
Nihon University School of Medicine Research Assistant
1986 - 1990
Assistante prof., Nihon Univesity, School of
1986 - 1990
Med.
of Sc.
Niigata University Faculty of Science Department of Biology Professor
2012.4
Niigata University Graduate School of Science and Technology Life and Food Sciences Life Sciences Professor
2012.4
Niigata University Faculty of Science Department of Biology Associate Professor
2004.4 - 2012.3
Hokkaido University 理学研究科博士課程 動物学
1984 - 1986
Hokkaido University Graduate School, Division of Natural Science
- 1984
Hokkaido University 理学研究科修士課程 動物学
1982 - 1984
Country: Japan
Hokkaido University Faculty of Science Department of Zoology
- 1982
Hokkaido University School of Science 生物学科
1977 - 1982
Country: Japan
日本発生生物学会
日本動物学会
A <i>myeloperoxidase</i> enhancer drives myeloid cell‐specific labeling in a transgenic frog line
Shiori Yamada‐Kondo, Ayame Ogawa, Miku Fukunaga, Yumi Izutsu, Takashi Kato, Mitsugu Maéno
Development, Growth & Differentiation 64 ( 7 ) 362 - 367 2022.9
Specific activation of the hb4 gene in the Xenopus oocyte through a Nobox-binding element located at the proximal promoter sequence. Reviewed
Nakamigawa M, Kondo T, Maéno M
Zygote 27 ( 4 ) 195 - 202 2019.6
Multiple origins of embryonic and tadpole myeloid cells in Xenopus laevis Reviewed
Yasutaka Imai, Keisuke Ishida, Maya Nemoto, Keisuke Nakata, Takashi Kato, Mitsugu Maeno
CELL AND TISSUE RESEARCH 369 ( 2 ) 341 - 352 2017.8
Nkx2.5 is involved in myeloid cell differentiation at anterior ventral blood islands in the Xenopus embryo Reviewed
Hiroyuki Sakata, Mitsugu Maeno
DEVELOPMENT GROWTH & DIFFERENTIATION 56 ( 8 ) 544 - 554 2014.10
Expression and localization of Rdd proteins in Xenopus embryo Reviewed
Jong-Chan Lim, Sayaka Kurihara, Rie Tamaki, Yutaka Mashima, Mitsugu Maéno
Anatomy and Cell Biology 47 ( 1 ) 18 - 27 2014
Distinct mechanisms control the timing of differentiation of two myeloid populations in Xenopus ventral blood islands Reviewed
Mitsugu Maeno, Kyogo Komiyama, Yoko Matsuzaki, Junichi Hosoya, Sayaka Kurihara, Hiroyuki Sakata, Yumi Izutsu
DEVELOPMENT GROWTH & DIFFERENTIATION 54 ( 2 ) 187 - 201 2012.2
A novel approach for a toxicity prediction model of environmental pollutants by using a quantitative structure-activity relationship method based on toxicogenomics. Reviewed
Hosoya J, Tamura K, Muraki N, Okumura H, Ito T, Maeno M
ISRN toxicology 2011 515724 2011
Involvement of Neptune in induction of the hatching gland and neural crest in the Xenopus embryo Reviewed
Takayuki Kurauchi, Yumi Izutsu, Mitsugu Maeno
DIFFERENTIATION 79 ( 4-5 ) 251 - 259 2010.4
Identification and expression of ventrally associated leucine-zipper (VAL) in Xenopus embryo Reviewed
Yuko Saito, Yuhta Takahashi, Yumi Izutsu, Mitsugu Maeno
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 54 ( 1 ) 203 - 208 2010
The keratin-related Ouroboros proteins function as immune antigens mediating tail regression in Xenopus metamorphosis Reviewed
Katsuki Mukaigasa, Akira Hanasaki, Mitsugu Maeno, Hiroshi Fujii, Shin-ichiro Hayashida, Mari Itoh, Makoto Kobayashi, Shin Tochinai, Masayuki Hatta, Kazuya Iwabuchi, Masanori Taira, Kazunori Onoe, Yumi Izutsu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106 ( 43 ) 18309 - 18314 2009.10
Involvement of AP-2rep in morphogenesis of the axial mesoderm in Xenopus embryo Reviewed
Yoshinari Saito, Masanori Gotoh, Yasutaka Ujiie, Yumi Izutsu, Mitsugu Maeno
CELL AND TISSUE RESEARCH 335 ( 2 ) 357 - 369 2009.2
A role of D domain-related proteins in differentiation and migration of embryonic cells in Xenopus laevis Reviewed
Tomoko Shibata, Yuhta Takahashi, Junichi Tasaki, Yuko Saito, Yumi Izutsu, Mitsugu Maeno
MECHANISMS OF DEVELOPMENT 125 ( 3-4 ) 284 - 298 2008.3
Nuclear envelope precursor vesicle targeting to chromatin is stimulated by protein phosphatase 1 in Xenopus egg extracts Reviewed
Hiromi Ito, Yuhei Koyama, Makoto Takano, Kohei Ishii, Mitsugu Maeno, Kazuhiro Furukawa, Tsuneyoshi Horigome
EXPERIMENTAL CELL RESEARCH 313 ( 9 ) 1897 - 1910 2007.5
Characterization of myeloid cells derived from the anterior ventral mesoderm in the Xenopus laevis embryo Reviewed
Sumihisa Tashiro, Ayako Sedohara, Makoto Asashima, Yumi Izutsu, Mitsugu Maeno
DEVELOPMENT GROWTH & DIFFERENTIATION 48 ( 8 ) 499 - 512 2006.10
Neptune is involved in posterior axis and tail formation in Xenopus embryogenesis Reviewed
M Takeda, T Kurauchi, T Yamazaki, Y Izutsu, M Maeno
DEVELOPMENTAL DYNAMICS 234 ( 1 ) 63 - 73 2005.9
Analyses of immune responses to ontogeny-specific antigens using an inbred strain of Xenopus laevis (J strain). Reviewed
Izutsu Y, Maéno M
Methods in molecular medicine 105 149 - 158 2005
Xenopus laevis macrophage migration inhibitory factor is essential for axis formation and neural development Reviewed
M Suzuki, Y Takamura, M Maeno, S Tochinai, D Iyaguchi, Tanaka, I, J Nishihira, T Ishibashi
JOURNAL OF BIOLOGICAL CHEMISTRY 279 ( 20 ) 21406 - 21414 2004.5
A BMP-4-dependent transcriptional control element in the 5 ' flanking region of Xenopus SCL gene Reviewed
T Sanada, M Park, A Araki, M Gotoh, Y Izutsu, M Maeno
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 310 ( 4 ) 1160 - 1167 2003.10
Regulatory signals and tissue interactions in the early hematopoietic cell differentiation in Xenopus laevis embryo Reviewed
M Maeno
ZOOLOGICAL SCIENCE 20 ( 8 ) 939 - 946 2003.8
Complementary expression of AP-2 and AP-2rep in ectodermal derivatives of Xenopus embryos Reviewed
M Gotoh, Y Izutsu, M Maeno
DEVELOPMENT GENES AND EVOLUTION 213 ( 7 ) 363 - 367 2003.7
Ontogenic emergence and localization of larval skin antigen molecule recognized by adult T cells of Xenopus laevis: Regulation by thyroid hormone during metamorphosis Reviewed
M Watanabe, M Ohshima, M Morohashi, M Maeno, Y Izutsu
DEVELOPMENT GROWTH & DIFFERENTIATION 45 ( 1 ) 77 - 84 2003.2
Larval antigen molecules recognized by adult immune cells of inbred Xenopus laevis: Partial characterization and implication in metamorphosis Reviewed
Y Izutsu, S Tochinai, M Maeno, K Iwabuchi, K Onoe
DEVELOPMENT GROWTH & DIFFERENTIATION 44 ( 6 ) 477 - 488 2002.12
Common and distinct signals specify the distribution of blood and vascular cell lineages in Xenopus laevis embryos Reviewed
F Iraha, Y Saito, K Yoshida, M Kawakami, Y Izutsu, IO Daar, M Maeno
DEVELOPMENT GROWTH & DIFFERENTIATION 44 ( 5 ) 395 - 407 2002.10
Two-step induction of primitive erythrocytes in Xenopus laevis embryos: signals from the vegetal endoderm and the overlying ectoderm Reviewed
M Kikkawa, M Yamazaki, Y Izutsu, M Maeno
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 45 ( 2 ) 387 - 396 2001.4
Expression and function of Xmsx-2B in dorso-ventral axis formation in gastrula embryos Reviewed
Onitsuka, I, M Takeda, M Maeno
ZOOLOGICAL SCIENCE 17 ( 8 ) 1107 - 1113 2000.11
Involvement of BMP-4/msx-1 and FGF pathways in neural induction in the Xenopus embryo Reviewed
A Ishimura, R Maeda, M Takeda, M Kikkawa, IO Daar, M Maeno
DEVELOPMENT GROWTH & DIFFERENTIATION 42 ( 4 ) 307 - 316 2000.8
Xenopus msx-1 regulates dorso-ventral axis formation by suppressing the expression of organizer genes. Reviewed
Takeda M, Saito Y, Sekine R, Onitsuka I, Maeda R, Maéno M
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 126 ( 2 ) 157 - 168 2000.6
Immunodetection of Xenopus bone morphogenetic protein-1 in adult and embryonic cells Reviewed
A Ishimura, GL Princler, JJ Lin, HF Kung, M Maeno
GROWTH FACTORS 16 ( 3 ) 171 - 177 1999
GATA-1 inhibits the formation of notochord and neural tissue in Xenopus embryo Reviewed
K Shibata, A Ishimura, M Maeno
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 252 ( 1 ) 241 - 248 1998.11
Xmsx-1 modifies mesodermal tissue pattern along dorsoventral axis in Xenopus laevis embryo Reviewed
R Maeda, A Kobayashi, R Sekine, JJ Lin, HF Kung, M Maeno
DEVELOPMENT 124 ( 13 ) 2553 - 2560 1997.7
XBMP-1B (Xtld), a Xenopus homolog of dorso-ventral polarity gene in Drosophila, modifies tissue phenotypes of ventral explants Reviewed
JJ Lin, R Maeda, RC Ong, JB Kim, LM Lee, HF Kung, M Maeno
DEVELOPMENT GROWTH & DIFFERENTIATION 39 ( 1 ) 43 - 51 1997.2
The role of BMP-4 and GATA-2 in the induction and differentiation of hematopoietic mesoderm in Xenopus laevis Reviewed
M Maeno, PE Mead, C Kelley, RH Xu, HF Kung, A Suzuki, N Ueno, LI Zon
BLOOD 88 ( 6 ) 1965 - 1972 1996.9
Involvement of Ras/Raf/AP-1 in BMP-4 signaling during Xenopus embryonic development Reviewed
RH Xu, ZG Dong, M Maeno, J Kim, A Suzuki, N Ueno, D Sredni, NH Colburn, HF Kung
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 93 ( 2 ) 834 - 838 1996.1
EXPRESSION OF AN AMPHIBIAN HOMOLOG OF THE EPH FAMILY OF RECEPTOR TYROSINE KINASES IS DEVELOPMENTALLY-REGULATED Reviewed
TL JONES, KARAVANOVA, I, M MAENO, RC ONG, HF KUNG, IO DAAR
ONCOGENE 10 ( 6 ) 1111 - 1117 1995.3
EXPRESSION OF THE HUMAN INTERLEUKIN-6 RECEPTOR ALPHA-CHAIN IN XENOPUS-LAEVIS OOCYTES Reviewed
M EGAWA, M MAENO, HF KUNG, M SCHWABE
CYTOKINE 7 ( 1 ) 83 - 88 1995.1
A TRUNCATED BONE MORPHOGENETIC PROTEIN-4 RECEPTOR ALTERS THE FATE OF VENTRAL MESODERM TO DORSAL MESODERM - ROLES OF ANIMAL POLE TISSUE IN THE DEVELOPMENT OF VENTRAL MESODERM Reviewed
M MAENO, RC ONG, A SUZUKI, N UENO, HF KUNG
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 91 ( 22 ) 10260 - 10264 1994.10
REGULATION OF PRIMARY ERYTHROPOIESIS IN THE VENTRAL MESODERM OF XENOPUS-GASTRULA EMBRYO - EVIDENCE FOR THE EXPRESSION OF A STIMULATORY FACTOR(S) IN ANIMAL POLE TISSUE Reviewed
M MAENO, RC ONG, Y XUE, S NISHIMATSU, N UENO, HF KUNG
DEVELOPMENTAL BIOLOGY 161 ( 2 ) 522 - 529 1994.2
CLONING AND EXPRESSION OF CDNA-ENCODING XENOPUS-LAEVIS BONE MORPHOGENETIC PROTEIN-1 DURING EARLY EMBRYONIC-DEVELOPMENT Reviewed
M MAENO, Y XUE, TI WOOD, RC ONG, HF KUNG
GENE 134 ( 2 ) 257 - 261 1993.12
MURINE STEM-CELL FACTOR STIMULATES ERYTHROPOIETIC DIFFERENTIATION OF VENTRAL MESODERM IN XENOPUS GASTRULA EMBRYO Reviewed
RC ONG, M MAENO, HF KUNG
EXPERIMENTAL CELL RESEARCH 205 ( 2 ) 326 - 330 1993.4
POSITIVE AND NEGATIVE REGULATION OF THE DIFFERENTIATION OF VENTRAL MESODERM FOR ERYTHROCYTES IN XENOPUS-LAEVIS Reviewed
M MAENO, RC ONG, HF KUNG
DEVELOPMENT GROWTH & DIFFERENTIATION 34 ( 5 ) 567 - 577 1992.10
ANALYSIS OF ALLOTOLERANCE IN THYMECTOMIZED XENOPUS RESTORED WITH SEMIALLOGENEIC THYMUS GRAFTS Reviewed
M MAENO, T NAKAMURA, S TOCHINAI, C KATAGIRI
TRANSPLANTATION 44 ( 2 ) 308 - 314 1987.8
TOLERANCE INDUCED BY GRAFTING SEMI-ALLOGENEIC ADULT SKIN TO LARVAL XENOPUS-LAEVIS - POSSIBLE INVOLVEMENT OF SPECIFIC SUPPRESSOR-CELL ACTIVITY Reviewed
T NAKAMURA, M MAENO, S TOCHINAI, C KATAGIRI
DIFFERENTIATION 35 ( 2 ) 108 - 114 1987
DIFFERENTIAL COMMITMENT OF HEMATOPOIETIC STEM-CELLS LOCALIZED IN DISTINCT COMPARTMENTS OF EARLY XENOPUS EMBRYOS Reviewed
C KATAGIRI, M MAENO, S TOCHINAI
CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY 20 315 - 323 1986
DIFFERENTIAL PARTICIPATION OF VENTRAL AND DORSOLATERAL MESODERMS IN THE HEMATOPOIESIS OF XENOPUS, AS REVEALED IN DIPLOID TRIPLOID OR INTERSPECIFIC CHIMERAS Reviewed
M MAENO, S TOCHINAI, C KATAGIRI
DEVELOPMENTAL BIOLOGY 110 ( 2 ) 503 - 508 1985
THE LOCALIZATION OF PRECURSOR CELLS FOR LARVAL AND ADULT HEMATOPOIETIC-CELLS OF XENOPUS-LAEVIS IN 2 REGIONS OF EMBRYOS Reviewed
M MAENO, A TODATE, C KATAGIRI
DEVELOPMENT GROWTH & DIFFERENTIATION 27 ( 2 ) 137 - 148 1985
ELICITATION OF WEAK IMMUNE-RESPONSE IN LARVAL AND ADULT XENOPUS-LAEVIS BY ALLOGRAFTED PITUITARY Reviewed
M MAENO, C KATAGIRI
TRANSPLANTATION 38 ( 3 ) 251 - 255 1984
Analyses of Immune Responses to Ontogeny-Specific Antigens using an Inbred Strain of Xenopus laevis (J Strain).
Developmental Hematopoiesis: Methods in Molecular Medicine, The Humana Press Inc. 2004
中胚葉系組織の分化機構-筋肉・血球の分化を誘導する分子相互作用-
両生類の発生生物学(北海道大学出版会) 1998
Physiological importance of Xenopus Neptune in development of neural and neural crest cells
Takayuki Kurauchi, Ai Araki, Yumi Izutsu, Mitsugu Maeno
ZOOLOGICAL SCIENCE 22 ( 12 ) 1464 - 1464 2005.12
Characterization of embryonic non-lymphoid leukocytes in Xenopus laevis
Sumihisa Tashiro, Yumi Izutsu, Mitsugu Maeno
ZOOLOGICAL SCIENCE 21 ( 12 ) 1301 - 1301 2004.12
Physiological function of AP-2rep in early Xenopus embryo
Yasutaka Ujiie, Masanori Gotoh, Yumi Izutsu, Mitsugu Maeno
ZOOLOGICAL SCIENCE 21 ( 12 ) 1298 - 1298 2004.12
Isolation of adult immune cells rejected larval tail skin grafts from inbred strain of Xeaopus
Ichiro Okano, Koki Fujiwara, Mitsugu Maeno, Yumi Izutsu
ZOOLOGICAL SCIENCE 21 ( 12 ) 1340 - 1340 2004.12
Fujii Hiroshi, Maeno Mitsugu, Izutsu Yumi
Zoological science 20 ( 12 ) 1560 - 1560 2003
Saito Y., Kawakami M., Takeda M., Maeno M.
Zoological science 16 81 - 81 1999
両生類胚における造血発生
細胞工学(透潤社) 17 1716 - 1721 1998
両生類胚における赤血球分化のプログラム「発生・神経研究の最前線96-'97」
実験医学(羊土社)増刊号 14 ( 8 ) 82 - 86 1996
MECHANISM OF ALLOTOLERANCE IN XENOPUS .1. CLONAL ABORTION IN EARLY THYMECTOMIZED FROGS RECONSTITUTED BY TRANSPLANTATION OF IRRADIATED SEMIALLOGENEIC THYMUS
M MAENO, T NAKAMURA, S TOCHINAI, C KATAGIRI
ZOOLOGICAL SCIENCE 2 ( 6 ) 902 - 902 1985
MECHANISM OF ALLOTOLERANCE IN XENOPUS .2. ACTIVE SUPPRESSION OF RESPONSE AGAINST SKINS GRAFTED IN LARVAL STAGES
T NAKAMURA, M MAENO, S TOCHINAI, C KATAGIRI
ZOOLOGICAL SCIENCE 2 ( 6 ) 902 - 902 1985
DIFFERENTIATION POTENCY OF EMBRYONIC XENOPUS CELLS AS REVEALED BY INJECTION INTO BLASTOCOEL OF EARLY GASTRULAE
M MAENO, C KATAGIRI
DEVELOPMENT GROWTH & DIFFERENTIATION 27 ( 4 ) 517 - 517 1985
ANALYSES OF TOLERANT STATE INDUCED BY INJECTION OF BLASTOMERES INTO GASTRULAE OF XENOPUS-LAEVIS
M MAENO, C KATAGIRI
ZOOLOGICAL SCIENCE 1 ( 6 ) 900 - 900 1984
EMBRYONIC LOCALIZATION OF LYMPHOID AND ERYTHROID PRECURSOR CELLS IN XENOPUS-LAEVIS
M MAENO, A TODATE, C KATAGIRI
DEVELOPMENT GROWTH & DIFFERENTIATION 26 ( 4 ) 394 - 394 1984
Regulation of amphibian hematopoiesis: the distribution of hematopoietic stem cells in the liver, spleen, and bone marrow.
Grant number:20K06729
2020.4 - 2023.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )
両生類で発見された新たな胚体起源に由来する骨髄球の性状と発生意義
2016 - 2018
System name:基盤研究(C)
前野 貢
Authorship:Principal investigator Grant type:Competitive
Effects of neonicotinoid insecticides on paddy organisms, covering from individuals to biocommunity level
Grant number:26281050
2014.4 - 2017.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
Sekijima Tsuneo
Grant amount:\16640000 ( Direct Cost: \12800000 、 Indirect Cost:\3840000 )
The effects of insecticides for paddy rice on paddy organisms were evaluated from individuals to biocommunity level. Neonicotinoid insecticide, Clothanidin, gave several negative effects to paddy biocommunity, and particularly caused a decrease of the amount of midges, dragonflies, and water fleas. Also, Nereistoxin insecticide, Cartap, indused several severe abnormalities on earlier development of frog embryo including Xenopus. The DNA microarray analysis suggested that disturbance of the genes expression related to morphogenesis and immune system was involved in teratogenic process of frogs.
両生類胚における血島の分化制御に関する総合的研究
2012 - 2014
System name:基盤研究(C)
前野 貢
Authorship:Principal investigator Grant type:Competitive
An acquired immune system involved in the tissue remodeling through self antigens
Grant number:23370059
2011.4 - 2015.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
IZUTSU YUMI, IWABUCHI Kazuya, ITO Michihiko, OKA Atsuko, MAENO Mitsugu
Grant amount:\19890000 ( Direct Cost: \15300000 、 Indirect Cost:\4590000 )
Vertebrate ontogenesis, including Xenopus frogs, shows morphological changes from embryonic- (larval-) organs into the adult counterparts. This process is called tissue remodeling. The most drastic remodeling is seen in the tadpole tail regression during amphibian metamorphosis. We have previously proposed a new model that the adult-type immune cells, which are newly differentiated during metamorphosis, recognize and remove own tail tissues as non-self. We have isolated two genes encoding the antigen proteins, Ouro1 and Ouro2, as targets for adult immune T cells. In this study, to clarify function of T cells vs these Ouro1 and Ouro2 antigens, we analyzed gain- and loss-of function of T cells. Results suggest that T cells are nessesorry and sufficient for the regression of tadpole tails expressing both Ouro1 and Ouro2 antigen proteins.
両生類胚の腹部血島の分化にかかわる新たな因子の同定と解析
2009 - 2011
System name:基盤研究(C)
前野 貢
Authorship:Principal investigator Grant type:Competitive
組織特異的発現を示すKruppel型転写因子の胚発生における役割
2004 - 2005
System name:基盤研究(C)
前野 貢
Authorship:Principal investigator Grant type:Competitive
Regulation of blood and vascular cell differentiation in embryos
Grant number:13680802
2001 - 2002
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
MAENO Mitsugu
Grant amount:\2500000 ( Direct Cost: \2500000 )
In order to determine the tissue interactions that propagate blood formation in amphibian embryogenesis, we used embryos that had the vegetal cytoplasm removed. These embryos displayed a marked enhancement of globin expression. Several lines of experimental evidence demonstrate the critical role of animal pole-derived ectoderm in blood cell formation: 1) Mesoderm derived from dorsal blastomeres injected with galactosidase mRNA (as a lineage tracer) expressed globin when interfaced with an animal pole-derived ectodermal layer: 2) Embryos in which the animal pole tissue had been removed by dissection at the blastula stage failed to express globin: 3) Exogastrulated embryos that lacked an interaction between the mesodermal and ectodermal layers failed to form blood cells, while, muscle cells were observed in these embryos. These observations suggest that default mesodermal cells need an additional BMP-4-dependent factor from the overlying ectoderm for further differentiation into a blood cell lineage.
Further experiments were done to elucidate the relationship between blood and vascular cell differentiation in the ventral blood island mesoderm. Using Xtie-2, as a vascular cell maker, it was shown that BMP (bone morphogenetic protein) signal is essential for both blood and vascular cell differentiation. On the other hand, FGF (fibroblast growth factor) signal was shown to control the distinct localization of vascular and blood cells. Therefore this study suggests that the early steps of blood and vascular cell differentiation are regulated by a common BMP-4-dependent signaling; however, distinct factor(s) such as FGF are involved in different distribution of these two cell lineages.
REGULATORY MECHANISMS OF DORSO-VENTRAL AXIS FORMATION IN AMPHIBIAN EMBRYO.
Grant number:09680721
1997 - 1999
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
MAENO Mitsugu
Grant amount:\2700000 ( Direct Cost: \2700000 )
I have investigated several cDNA clones, which encode for ventralizing agents. Among these factors, the roles of msx-1 and msx-2 in the dorsoventral axis formation have been extensively analyzed. These transcriptional repressor proteins were expressed in presumptive ectoderm and mesoderm ventrally, but not dorsally, at gastrula stage. Both msx-1 and msx-2 possessed a similar ventralizing activity, but that of msx-2 was higher than of msx-1, as revealed by the injection of RNA into dorsal blastomeres. The secondary axis induced by the injection of tBR was rescued by simultaneous injection of msx-1/msx-2. Furthermore, if VP-16/msx-1 fusion RNA, which was expected to function as a dominant negative msx-1, was injected into the ventral blastomeres, partial Secondary axis was formed. Therefore, msx-1 and msx-2 transduce a main tract of BMP-4 signaling. Msx factors also have a role in neural tissue induction in ectodermal cells. If msx-1 was coinjected with neural inducer genes such as noggin or cerberus, the expression of N-CAM was completely suppressed. As also shown in tBR (which directly induces neural tissue in animal cap), VP 16/msx-1 fusion RNA was able to induce neural tissue directly in animal cap cells. The results indicate that msx proteins play a central role in establishing dorso-ventral axis and in neural tissue formation in gastrulating embryo, by antagonizing the expression of organizer genes. The expression and function of GATA-1 (another transcriptional regulator) and BMP-1 (metalloprotease) in Xenopus embryo have been also investigated in this study.
Molecular mechanism of hematopoietic and angiopoietic differentiation in embryo
1992 - 2004
Grant type:Competitive
Experimental Infection and Characterization of Epidemic Non-A, Non-B Hepatitis Virus.
Grant number:63480146
1988 - 1990
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for General Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
SHIKATA Toshio, MAENO Mitsugu, SUZUKI Koyu, ESUMI Mariko, UCHIDA Toshikazu
Grant amount:\7000000 ( Direct Cost: \7000000 )
An attempt was made to establish the animal model of epidemic non-A, non-B hepatitis. Cynomolgus macaca was a good animal model, because if we inoculate enough amount virus intravenously, almost all monkeys developed acute hepatits. Serial transmission experiments were also done successfully. We have found large amount of virus excrete into bile from 10 days after inoculation until peak of transaminase. In early stage, virus scattered in the bile but next stage virus particles spontaneously aggregated probably due to secretory IgA. We already cloned viral RNA from lambda gt11 cDNA library using immunoscreening method. We also confirmed viremia in incubation stage by PCR method. Now we are preparing antibody assay kit using recombinant peptide. We are attempting to debelop recombinant vaccine too.
Serological diagnosis of non-A, non-B hepatitis.
Grant number:62870018
1987 - 1989
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Developmental Scientific Research
Awarding organization:Japan Society for the Promotion of Science
SHIKATA Toshio, MAENO Mitsugu, SUZUKI Koyu, ESUMI Mariko, UCHIDA Toshikazu, SHIMIZU Yohko
Grant amount:\7600000 ( Direct Cost: \7600000 )
An attempt was made to establish an antigen antibody assay system associated with non-A, non-B hepatitis. We have established monoclonal antibody from convalescent chimpanzee lymphocytes by EV virus transformation method. We found the corresponding antigen in the chimpanzee liver infected with non-A, non-B hepatitis virus and never found in the liver of the chimpanzees infected hepatitis A and B virus.
However, cDNA coding this antigen hybridized with somatic genome, then this antigen was not coded by the viral genome.
Then we cloned hepatitis C Genome from Japanese donor blood by immunoscreening and sequenced. Sequence of this Japanese clone of HCV is different from Chiron's clone. Hemology is only 74% in region NS5. We established EIA assay system of the antibody against HCV.
And we also established cDNA assay system of HCV genom by PCR method.
Development of a cytofluorometry system for tissue culture cells in vivo
Grant number:62870017
1987 - 1989
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Developmental Scientific Research
Awarding organization:Japan Society for the Promotion of Science
ASHIHARA Tsukasa, KONISHI Eiichi, HOSOKAWA Youhei
Grant amount:\3900000 ( Direct Cost: \3900000 )
Electron microscopic investigation of ribonucleic acid in nuclear particulate aggregates of hepatitis NANB by means of nuclease-gold complexes.
Grant number:62570315
1987 - 1988
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for General Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
KOJIMA Takashi, SUGITANI Masahiko, SUZUKI Koyu, MAENO Mitsugu
Grant amount:\2100000 ( Direct Cost: \2100000 )
The ultrastructural localization of hepatitis delta antioen (HDAg) and ribonucleic acid (RNA) was investigated by immunoperoxidase electron microscopy and by enzyme electron microscopy of RNase-gold complexes on liver biopsies from seven patients with hepatitis D. HDAh was mainly localized in the nucleus, and sometimes in the cytoplasm of hepatocytes. Ultrastructurally, intranuclear HDAg was found on nuclear particulate structures measuring 20-to 30-nm in diameter. Intranuclear RNA visualized with gold particles was found in high amounts in the nucleolus, to a small extent in the chromatin area and in addition also on nuclear particulate structures. These findings suggest that intranuclear aggregates of irregular granular particulate structures in hepatitis D are the internal component of HDV particles in blood.
In order to clarify the hepatitis non-A, non-B virus (H-NANB-V), enzyme electron microscopy of RNase-gold complexes on liver biopsies from two chimpanzees experimentally infected hepatitis HANB. Nuclear particulate structures, curved membrane and double unit membrane were found in all two biopsy speciment. Although the nuclear particulate structures were closely associated with RNA as in hepatitis D, curved membrane and double unit membrane were not related with nucleic acids of RNA and deoxi-ribonucleic acid (DNA). To date, no particulate structures specifically associated with H-NANB-V were demonstrated by enzyme electron microscopy.
地域創生科学演習
生物学実習I
生物学実習II
基礎生物科学実習II
基礎生物科学実習I
理学スタディ・スキルズ
生物学特論II
先端科学技術総論
日本事情自然系A
胚発生学特論
課題研究II(生物学)
生物学総合演習
自然科学基礎実験
課題研究I(生物学)
生物学基礎実習a
生物学基礎演習
生物学特論Ⅲ
科学・技術と社会
Biology Today
生命科学への招待(生物学学習法)
発生生物学II
課題研究I(生物学科)
生命・食料科学特定研究BⅡ
研究発表演習(中間発表)
生命・食料科学セミナーBⅡ
文献詳読Ⅱ
研究発表(博士)演習(学会発表含む)
生命・食料科学特定研究BⅠ
文献詳読Ⅰ
基礎生命科学(博士)演習(中間発表)
生命・食料科学セミナーBⅠ
外国語論文解説・討論Ⅰ
リサーチキャンプ
生命・食料科学博士特定研究Ⅰ
生命・食料科学博士セミナーⅠ
自然科学総論Ⅳ
生物学特論IV B
基礎生物科学実習II
日本事情自然系A
生物学実験 I
発生生物学
生物学特論V A
動物形態発生学実習
発生生物学演習
基礎細胞遺伝学
生物学基礎B
基礎生物科学実習I
胚発生学
胚発生学特論
課題研究II
発生のプログラム
課題研究I
分子生物学実習