2021/12/04 更新

写真a

ヒシダ リユウイチ
菱田 竜一
HISHIDA Ryuichi
所属
脳研究所 生命科学リソース研究センター 准教授
職名
准教授
外部リンク

学位

  • 博士(理学) ( 1997年3月   京都大学 )

  • 修士(理学) ( 1992年3月   京都大学 )

研究キーワード

  • フラビン蛋白蛍光イメージング

  • 脳機能イメージング

  • 高次連合野

  • マウス

  • ゼブラフィッシュ

  • 脳生理学

  • 病態生理学

  • 老化

  • アフリカメダカ

  • ミトコンドリア

  • 加齢関連疾患

研究分野

  • ライフサイエンス / 神経科学一般

  • ライフサイエンス / 分子生物学

経歴(researchmap)

  • 新潟大学 脳研究所 生命科学リソース研究センター / 脳科学リソース研究部門 脳病態解析分野   准教授

    2020年4月 - 現在

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  • 新潟大学   脳研究所 基礎神経科学部門 システム脳生理学分野   准教授

    2007年7月 - 2020年3月

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  • 新潟大学   脳研究所 基礎神経科学部門 システム脳生理学分野   助教

    1999年4月 - 2007年6月

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経歴

  • 新潟大学   脳研究所 生命科学リソース研究センター   准教授

    2020年4月 - 現在

  • 新潟大学   脳研究所 基礎神経科学部門   准教授

    2007年7月 - 2020年3月

  • 新潟大学   医歯学総合研究科 生体機能調節医学専攻   准教授

    2007年7月 - 2020年3月

  • 新潟大学   医歯学総合研究科 医科学専攻   准教授

    2007年7月 - 2020年3月

  • 新潟大学   脳研究所 基礎神経科学部門   助教

    1999年4月 - 2007年6月

学歴

  • 京都大学大学院   理学研究科 博士後期課程 (生物物理学専攻)

    1992年4月 - 1997年3月

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  • 京都大学大学院   理学研究科 修士課程 (生物物理学専攻)

    1990年4月 - 1992年3月

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  • 京都大学 理学部

    1986年4月 - 1990年3月

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所属学協会

 

論文

  • Auditory cortical activity elicited by infrared laser irradiation from the outer ear in Mongolian gerbils. 査読 国際誌

    Yuta Tamai, Yuki Ito, Takafumi Furuyama, Kensuke Horinouchi, Nagomi Murashima, Itsuki Michimoto, Ryuichi Hishida, Katsuei Shibuki, Shizuko Hiryu, Kohta I Kobayasi

    PloS one   15 ( 10 )   e0240227   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Infrared neural stimulation has been studied for its potential to replace an electrical stimulation of a cochlear implant. No studies, however, revealed how the technic reliably evoke auditory cortical activities. This research investigated the effects of cochlear laser stimulation from the outer ear on auditory cortex using brain imaging of activity-dependent changes in mitochondrial flavoprotein fluorescence signal. An optic fiber was inserted into the gerbil's ear canal to stimulate the lateral side of the cochlea with an infrared laser. Laser stimulation was found to activate the identified primary auditory cortex. In addition, the temporal profile of the laser-evoked responses was comparable to that of the auditory responses. Our results indicate that infrared laser irradiation from the outer ear has the capacity to evoke, and possibly manipulate, the neural activities of the auditory cortex and may substitute for the present cochlear implants in future.

    DOI: 10.1371/journal.pone.0240227

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  • Reciprocal connectivity between secondary auditory cortical field and amygdala in mice. 査読 国際誌

    Tsukano H, Hou X, Horie M, Kitaura H, Nishio N, Hishida R, Takahashi K, Kakita A, Takebayashi H, Sugiyama S, Shibuki K

    Scientific reports   9 ( 1 )   19610 - 19610   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-019-56092-9

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  • Associative responses to visual shape stimuli in the mouse auditory cortex 査読

    Ogi M, Yamagishi T, Tsukano H, Nishio N, Hishida R, Takahashi K, Horii A, Shibuki K

    PLoS One   14 ( 10 )   e0223242   2019年9月

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  • Acute spatial spread of NO-mediated potentiation during hindpaw ischaemia in mice. 査読 国際誌

    Onishi T, Watanabe T, Sasaki M, Kamiya Y, Horie M, Tsukano H, Hishida R, Kohno T, Takebayashi H, Baba H, Shibuki K

    The Journal of Physiology   597 ( 13 )   3441 - 3455   2019年5月

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    記述言語:英語   掲載種別:学位論文(博士)  

    DOI: 10.1113/JP277615

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  • Feedback inhibition derived from the posterior parietal cortex regulates the neural properties of the mouse visual cortex. 査読

    Hishida R, Horie M, Tsukano H, Tohmi M, Yoshitake K, Meguro R, Takebayashi H, Yanagawa Y, Shibuki K

    The European journal of neuroscience   50   2970 - 2987   2019年4月

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    担当区分:筆頭著者, 責任著者  

    DOI: 10.1111/ejn.14424

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  • Direct Relay Pathways from Lemniscal Auditory Thalamus to Secondary Auditory Field in Mice. 査読

    Ohga S, Tsukano H, Horie M, Terashima H, Nishio N, Kubota Y, Takahashi K, Hishida R, Takebayashi H, Shibuki K

    Cerebral cortex (New York, N.Y. : 1991)   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/cercor/bhy234

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  • Higher visual responses in the temporal cortex of mice. 査読 国際誌

    Nishio N, Tsukano H, Hishida R, Abe M, Nakai J, Kawamura M, Aiba A, Sakimura K, Shibuki K

    Scientific reports   8 ( 1 )   11136 - 11136   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-018-29530-3

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  • Molecular diversity of clustered protocadherin-α required for sensory integration and short-term memory in mice. 査読

    Yamagishi T, Yoshitake K, Kamatani D, Watanabe K, Tsukano H, Hishida R, Takahashi K, Takahashi S, Horii A, Yagi T, Shibuki K

    Scientific reports   8 ( 1 )   9616   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-018-28034-4

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    その他リンク: http://www.nature.com/articles/s41598-018-28034-4

  • Tomographic optical imaging of cortical responses after crossing nerve transfer in mice. 査読

    Maniwa K, Yamashita H, Tsukano H, Hishida R, Endo N, Shibata M, Shibuki K

    PloS one   13 ( 2 )   e0193017   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1371/journal.pone.0193017

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  • Reconsidering Tonotopic Maps in the Auditory Cortex and Lemniscal Auditory Thalamus in Mice 査読

    Hiroaki Tsukano, Masao Horie, Shinpei Ohga, Kuniyuki Takahashi, Yamato Kubota, Ryuichi Hishida, Hirohide Takebayashi, Katsuei Shibuki

    FRONTIERS IN NEURAL CIRCUITS   11   14   2017年2月

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    記述言語:英語   出版者・発行元:FRONTIERS MEDIA SA  

    The auditory thalamus and auditory cortex (AC) are pivotal structures in the central auditory system. However, the thalamocortical mechanisms of processing sounds are largely unknown. Investigation of this process benefits greatly from the use of mice because the mouse is a powerful animal model in which various experimental techniques, especially genetic tools, can be applied. However, the use of mice has been limited in auditory research, and thus even basic anatomical knowledge of the mouse central auditory system has not been sufficiently collected. Recently, optical imaging combined with morphological analyses has enabled the elucidation of detailed anatomical properties of the mouse auditory system. These techniques have uncovered fine AC maps with multiple frequency-organized regions, each of which receives pointto- point thalamocortical projections from different origins inside the lemniscal auditory thalamus, the ventral division of the medial geniculate body (MGv). This precise anatomy now provides a platform for physiological research. In this mini review article, we summarize these recent achievements that will facilitate physiological investigations in the mouse auditory system.

    DOI: 10.3389/fncir.2017.00014

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  • Independent tonotopy and thalamocortical projection patterns in two adjacent parts of the classical primary auditory cortex in mice 査読

    Hiroaki Tsukano, Masao Horie, Kuniyuki Takahashi, Ryuichi Hishida, Hirohide Takebayashi, Katsuei Shibuki

    NEUROSCIENCE LETTERS   637   26 - 30   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Amid recent amendment of delineation of a mouse auditory cortical map, a caudal auditory field, originally defined as the primary auditory cortex (AI), was divided into the AI and dorsomedial field (DM), based on distinct high frequency areas. A low frequency area was not previously established in the DM because responses to low frequency tones were weak in this area. This may lead to the misconception that the DM is an atypical region that lacks a low frequency band. In the current study, we confirmed that the DM has a low frequency area that is completely independent from the AI. First, we conducted flavoprotein fluorescence imaging with improved signal to noise ratio and revealed the presence of two separated low frequency areas in the AI and DM. Next, we injected a retrograde neural tracer along the tonotopic axis of the AI or DM to reveal the thalamic origins in the ventral division of the medial geniculate body (MGv). We found that neurons projecting to low frequency areas of the AI and DM occupied different locations within the MGv and mutually independent topographic organizations consisting of thalamic neurons projecting to the AI or DM. These results indicate that the AI and DM have distinct low frequency areas with distinct thalamic projections from the MGv. Our findings reaffirm that the AI and DM should be regarded as independent regions in the mouse auditory cortex. (C) 2016 The Authors. Published by Elsevier Ireland Ltd.

    DOI: 10.1016/j.neulet.2016.11.062

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  • Auditory cortical field coding long-lasting tonal offsets in mice. 査読

    Baba H, Tsukano H, Hishida R, Takahashi K, Horii A, Takahashi S, Shibuki K

    Scientific reports   6 ( 1 )   34421   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep34421

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    その他リンク: http://www.nature.com/articles/srep34421.pdf

  • Quantitative map of multiple auditory cortical regions with a stereotaxic fine-scale atlas of the mouse brain. 査読

    Tsukano H, Horie M, Hishida R, Takahashi K, Takebayashi H, Shibuki K

    Scientific reports   6   22315   2016年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep22315

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  • Spinal mechanisms underlying potentiation of hindpaw responses observed after transient hindpaw ischemia in mice. 査読 国際誌

    Watanabe T, Sasaki M, Komagata S, Tsukano H, Hishida R, Kohno T, Baba H, Shibuki K

    Scientific reports   5   11191 - 11191   2015年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep11191

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  • Delineation of a frequency-organized region isolated from the mouse primary auditory cortex. 査読

    Tsukano H, Horie M, Bo T, Uchimura A, Hishida R, Kudoh M, Takahashi K, Takebayashi H, Shibuki K

    Journal of neurophysiology   113 ( 7 )   2900 - 20   2015年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1152/jn.00932.2014

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  • Impaired clustered protocadherin-α leads to aggregated retinogeniculate terminals and impaired visual acuity in mice. 査読

    Meguro R, Hishida R, Tsukano H, Yoshitake K, Imamura R, Tohmi M, Kitsukawa T, Hirabayashi T, Yagi T, Takebayashi H, Shibuki K

    Journal of neurochemistry   133 ( 1 )   66 - 72   2015年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/jnc.13053

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  • Multimodal cortical sensory pathways revealed by sequential transcranial electrical stimulation in mice. 査読

    Hishida R, Kudoh M, Shibuki K

    Neuroscience research   87   49 - 55   2014年10月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.neures.2014.07.004

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  • The Extrageniculate Visual Pathway Generates Distinct Response Properties in the Higher Visual Areas of Mice 査読

    Manavu Tohmi, Reiko Meguro, Hiroaki Tsukano, Ryuichi Hishida, Katsuei Shibuki

    CURRENT BIOLOGY   24 ( 6 )   587 - 597   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

    Background: Visual information conveyed through the extra-geniculate visual pathway, which runs from the retina via the superior colliculus (SC) and the lateral posterior nucleus (LPN) of the thalamus to the higher visual cortex, plays a critical role in the visual capabilities of many mammalian species. However, its functional role in the higher visual cortex remains unclear. Here, we observed visual cortical area activity in anesthetized mice to evaluate the role of the extrageniculate pathway on their specialized visual properties.
    Results: The preferred stimulus velocities of neurons in the higher visual areas (lateromedial [LM], anterolateral [AL], anteromedial [AM], and rostrolateral [RL] areas) were measured using flavoprotein fluorescence imaging and two-photon calcium imaging and were higher than those in the primary visual cortex (V1). Further, the velocity-tuning properties of the higher visual areas were different from each other. The response activities in these areas decreased after V1 ablation; however, the visual properties' differences were preserved. After SC destruction, these preferences for high velocities disappeared, and their tuning profiles became similar to that of the V1, whereas the tuning profile of the V1 remained relatively normal. Neural tracer experiments revealed that each of these higher visual areas connected with specific subregions of the LPN.
    Conclusions: The preservation of visual property differences among the higher visual areas following V1 lesions and their loss following SC lesions indicate that pathways from the SC through the thalamus to higher cortical areas are sufficient to support these differences.

    DOI: 10.1016/j.cub.2014.01.061

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  • Visual map shifts based on whisker-guided cues in the young mouse visual cortex 査読

    Kohei Yoshitake, Hiroaki Tsukano, Manavu Tohmi, Seiji Komagata, Ryuichi Hishida, Takeshi Yagi, Katsuei Shibuki

    Cell Reports   5 ( 5 )   1365 - 1374   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Mice navigate nearby space using their vision and whiskers, and young mice learn to integrate these heterogeneous inputs in perceptual space. We found that cortical responses were depressed in the primary visual cortex of young mice after wearing a monocular prism. This depression was uniformly observed in the primary visual cortex and was eliminated by whisker trimming or lesions in the posterior parietal cortex. Compensatory visual map shifts of responses elicited via the eye that had worn the prism were also observed. As a result, cortical responses elicited via each eye were clearly separated when a visual stimulus was placed in front of the mice. A comparison of response areas before and after prism wearing indicated that the map shifts were produced by depression with spatial eccentricity. Visual map shifts based on whisker-guided cues may serve as a model for investigating the cellular and molecular mechanisms underlying higher sensory integration in the mammalian brain.

    DOI: 10.1016/j.celrep.2013.11.006

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  • Age-related deterioration of cortical responses to slow FM sounds in the auditory belt region of adult C57BL/6 mice 査読

    Hiroaki Tsukano, Masao Horie, Yuusuke Honma, Shinpei Ohga, Ryuichi Hishida, Hirohide Takebayashi, Sugata Takahashi, Katsuei Shibuki

    NEUROSCIENCE LETTERS   556   204 - 209   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    To compare age-related deterioration of neural responses in each subfield of the auditory cortex in C57BL/6 mice, we evaluated amplitudes of tonal responses in young (5-11 weeks old) and adult (16-23 weeks old) groups using transcranial flavoprotein fluorescence imaging. Cortical responses to 20-kHz amplitude-modulated (AM) sounds, which were mainly found in the anterior auditory field (AAF) and the primary auditory cortex (AI) of the core region, were not markedly different between the two groups. In contrast, cortical responses to direction reversal of slow frequency-modulated (FM) sounds, which were mainly found in the ultrasonic field (UF), were significantly disrupted in the adult group compared with those in the young group. To investigate the mechanisms underlying such age-related deterioration, biotinylated dextran amine (BDA) was injected into UF. The number of retrograde labeled neurons in the dorsal division of the medial geniculate body (MGd) was markedly reduced in the adult group compared with that in the young group. These results strongly suggest that cortical responses to FM direction reversal in UF of adult C57BL/6 mice are mainly deteriorated by loss of non-lemniscal thalamic inputs from MGd to UF due to aging. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2013.10.015

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  • Dual compartments of the ventral division of the medial geniculate body projecting to the core region of the auditory cortex in C57BL/6 mice 査読

    Masao Horie, Hiroaki Tsukano, Ryuichi Hishida, Hirohide Takebayashi, Katsuei Shibuki

    NEUROSCIENCE RESEARCH   76 ( 4 )   207 - 212   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    We investigated precise projection patterns from the ventral division of the medial geniculate body (MGv) projecting to the core region of the auditory cortex in C57BL/6 mice. The core region in mice comprises two different tonotopically organized areas, the anterior auditory field (AAF) and the primary auditory cortex (AI). In the present study, AAF and AI were functionally identified using flavoprotein fluorescence imaging. Biotinylated dextran amine (BDA) was injected iontophoretically into the tonotopic bands to 5 kHz and 20 kHz in AAF, and those to 5 kHz, 10 kHz, and 20 kHz in AI for staining MGv neurons projecting to the injected sites. MGv neurons projecting to AAF were found in the medial part of MGv, while MGv neurons projecting to AI were found in the lateral part. In the medial part of MGv, areas projecting to 5-20 kHz bands in AAF were aligned along the medio lateral axis. In the lateral part of MGv, areas projecting to 5-20 kHz bands in AI were aligned along the dorso ventral axis. These results indicate that AAF and AI receive auditory information via two different MGv compartments with independent tonotopic axes, respectively. (C) 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/j.neures.2013.05.004

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  • Auditory Cortical Areas Activated by Slow Frequency-Modulated Sounds in Mice 査読

    Yuusuke Honma, Hiroaki Tsukano, Masao Horie, Shinsuke Ohshima, Manavu Tohmi, Yamato Kubota, Kuniyuki Takahashi, Ryuichi Hishida, Sugata Takahashi, Katsuei Shibuki

    PLOS ONE   8 ( 7 )   e68113   2013年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Species-specific vocalizations in mice have frequency-modulated (FM) components slower than the lower limit of FM direction selectivity in the core region of the mouse auditory cortex. To identify cortical areas selective to slow frequency modulation, we investigated tonal responses in the mouse auditory cortex using transcranial flavoprotein fluorescence imaging. For differentiating responses to frequency modulation from those to stimuli at constant frequencies, we focused on transient fluorescence changes after direction reversal of temporally repeated and superimposed FM sweeps. We found that the ultrasonic field (UF) in the belt cortical region selectively responded to the direction reversal. The dorsoposterior field (DP) also responded weakly to the reversal. Regarding the responses in UF, no apparent tonotopic map was found, and the right UF responses were significantly larger in amplitude than the left UF responses. The half-max latency in responses to FM sweeps was shorter in UF compared with that in the primary auditory cortex (A1) or anterior auditory field (AAF). Tracer injection experiments in the functionally identified UF and DP confirmed that these two areas receive afferent inputs from the dorsal part of the medial geniculate nucleus (MG). Calcium imaging of UF neurons stained with fura-2 were performed using a two-photon microscope, and the presence of UF neurons that were selective to both direction and direction reversal of slow frequency modulation was demonstrated. These results strongly suggest a role for UF, and possibly DP, as cortical areas specialized for processing slow frequency modulation in mice.

    DOI: 10.1371/journal.pone.0068113

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  • Restoration of Contralateral Representation in the Mouse Somatosensory Cortex after Crossing Nerve Transfer 査読

    Haruyoshi Yamashita, Shanlin Chen, Seiji Komagata, Ryuichi Hishida, Takuji Iwasato, Shigeyoshi Itohara, Takeshi Yagi, Naoto Endo, Minoru Shibata, Katsuei Shibuki

    PLOS ONE   7 ( 4 )   e35676   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Avulsion of spinal nerve roots in the brachial plexus (BP) can be repaired by crossing nerve transfer via a nerve graft to connect injured nerve ends to the BP contralateral to the lesioned side. Sensory recovery in these patients suggests that the contralateral primary somatosensory cortex (S1) is activated by afferent inputs that bypassed to the contralateral BP. To confirm this hypothesis, the present study visualized cortical activity after crossing nerve transfer in mice through the use of transcranial flavoprotein fluorescence imaging. In naive mice, vibratory stimuli applied to the forepaw elicited localized fluorescence responses in the S1 contralateral to the stimulated side, with almost no activity in the ipsilateral S1. Four weeks after crossing nerve transfer, forepaw stimulation in the injured and repaired side resulted in cortical responses only in the S1 ipsilateral to the stimulated side. At eight weeks after crossing nerve transfer, forepaw stimulation resulted in S1 cortical responses of both hemispheres. These cortical responses were abolished by cutting the nerve graft used for repair. Exposure of the ipsilateral S1 to blue laser light suppressed cortical responses in the ipsilateral S1, as well as in the contralateral S1, suggesting that ipsilateral responses propagated to the contralateral S1 via cortico-cortical pathways. Direct high-frequency stimulation of the ipsilateral S1 in combination with forepaw stimulation acutely induced S1 bilateral cortical representation of the forepaw area in naive mice. Cortical responses in the contralateral S1 after crossing nerve transfer were reduced in cortex-restricted heterotypic GluN1 (NMDAR1) knockout mice. Functional bilateral cortical representation was not clearly observed in genetically manipulated mice with impaired cortico-cortical pathways between S1 of both hemispheres. Taken together, these findings strongly suggest that activity-dependent potentiation of cortico-cortical pathways has a critical role for sensory recovery in patients after crossing nerve transfer.

    DOI: 10.1371/journal.pone.0035676

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  • Detection of virtual pitch up to 5 kHz by mice 査読

    Hiroaki Tsukano, Ryuichi Hishida, Katsuei Shibuki

    NEUROSCIENCE RESEARCH   71 ( 2 )   140 - 144   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Natural sounds consist of a component at the fundamental frequency (f0) and its overtones. Pitch is perceived at f0, even when spectral energy at f0 is missing. This missing f0, or 'virtual pitch', is thought to be detected in the auditory cortex and related cortical areas, but the precise neural mechanisms are unknown. One possibility is that virtual pitch can be retrieved from the periodicity of sound waveforms. However, this mechanism requires the temporal accuracy in periodicity detection, and so far the detection of virtual pitch has only been demonstrated at frequencies lower than 1 kHz. We investigated the ability of mice to detect virtual pitch up to 5 kHz using a two-step sound discrimination test. In the first step of this test, mice were trained to discriminate between tone bursts at 2.5 and 5 kHz. In the second step, we tested the ability of mice to discriminate between virtual pitches at 2.5 kHz and at 5 kHz. It was demonstrated that the performance of mice to discriminate between virtual pitches at 2.5 and 5 kHz was significantly affected by previous discrimination learning between tone bursts, indicating that mice can detect virtual pitch up to 5 kHz. (C) 2011 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/j.neures.2011.06.005

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  • Transcranial electrical stimulation of cortico-cortical connections in anesthetized mice 査読

    Ryuichi Hishida, Kenji Watanabe, Masaharu Kudoh, Katsuei Shibuki

    JOURNAL OF NEUROSCIENCE METHODS   201 ( 2 )   315 - 321   2011年10月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We developed a technique of transcranial electrical stimulation (TES) to investigate cortico-cortical connections in mice. After the skull was shaved with the blade of a dental bar, a blunt tip of a needle was gently pushed onto the thinned skull. The skull was deformed by the force, and the subarachnoid space between the skull and the cortex was minimized around the needle tip. Under these conditions, stimulus currents applied to the needle directly flowed into the cortex through the thinned skull. Cortico-cortical functional connections stimulated by this method were visualized by transcranial flavoprotein fluorescence imaging. The cortical responses evoked by TES exhibited spatial and temporal activity patterns comparable to those elicited by a conventional method, in which an electrode is directly inserted into superficial cortical layers. A comparison of the two methods revealed that TES required a slightly stronger stimulus intensity and preferentially activated superficial layers of the cortex compared with the conventional method. Using the new method, we revealed the presence of reciprocal cortico-cortical functional connections between lateral and medial parts of higher visual cortices in mice. This new method combined with transcranial flavoprotein fluorescence imaging allowed us to activate cortico-cortical pathways arising from the primary sensory areas and investigate sensory information flow in the mouse cerebral cortex. (C) 2011 Elsevier B.V. All rights reserved.

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  • Nociceptive cortical responses during capsaicin-induced tactile allodynia in mice with spinal dorsal column lesioning 査読

    Seiji Komagata, Keisuke Tamaki, Ryuichi Hishida, Nobuaki Takeshita, Katsuei Shibuki

    NEUROSCIENCE RESEARCH   69 ( 4 )   348 - 351   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    We investigated nociceptive cortical responses using transcranial flavoprotein fluorescence imaging in anesthetized mice with capsaicin-induced allodynia. Tactile stimuli applied to the hindpaw produced fluorescence increases in the contralateral somatosensory cortex of naive mice. Lesioning of the ipsilateral dorsal column in the spinal cord abolished most of the cortical responses. However, the responses to the same tactile stimuli appeared again after capsaicin was injected into the hindpaw.The capsaicin treatment reduced the thresholds of the hindpaw withdrawal responses. These findings strongly suggest that the responses to tactile stimuli in the lesioned mice after capsaicin injection represented nociceptive cortical responses. (c) 2011 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

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  • Initial phase of neuropathic pain within a few hours after nerve injury in mice. 査読

    Komagata Seiji, Chen Shanlin, Suzuki Akiko, Yamashita Haruyoshi, Hishida Ryuichi, Maeda Takeyasu, Shibata Minoru, Shibuki Katsuei

    J Neurosci   31 ( 13 )   4896 - 4905   2011年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We tested a hypothesis that the spinal plasticity induced within a few hours after nerve injury may produce changes in cortical activities and an initial phase of neuropathic pain. Somatosensory cortical responses elicited by vibratory stimulation were visualized by transcranial flavoprotein fluorescence imaging in mice. These responses were reduced immediately after cutting the sensory nerves. However, the remaining cortical responses mediated by nearby nerves were potentiated within a few hours after nerve cutting. Nerve injury induces neuropathic pain. In the present study, mice exhibited tactile allodynia 1-2 weeks after nerve injury. Lesioning of the ipsilateral dorsal column, mediating tactile cortical responses, abolished somatic cortical responses to tactile stimuli. However, nontactile cortical responses appeared in response to the same tactile stimuli within a few hours after nerve injury, indicating that tactile allodynia was acutely initiated. We investigated the trigger mechanisms underlying the cortical changes. Endogenous glial cell line-derived neurotrophic factor (GDNF), found in the Meissner corpuscles, induced basal firing approximately 0.1 Hz or less in its

    DOI: 10.1523/JNEUROSCI.6753-10.2011

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  • Timing-dependent effects of whisker trimming in thalamocortical slices including the mouse barrel cortex. 査読

    Watanabe K, Kamatani D, Hishida R, Shibuki K

    Brain Res   1385   93 - 106   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.brainres.2011.02.026

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  • Cortical depression in the mouse auditory cortex after sound discrimination learning 査読

    Shinsuke Ohshima, Hiroaki Tsukano, Yamato Kubota, Kuniyuki Takahashi, Ryuichi Hishida, Sugata Takahashi, Katsuei Shibuki

    NEUROSCIENCE RESEARCH   67 ( 1 )   51 - 58   2010年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Cortical responses after sound discrimination learning were investigated using transcranial flavoprotein fluorescence imaging in mice. Water-deprived mice were trained to discriminate between rewarded (S+) and unrewarded (S-) sound stimuli. After the learning, they were anesthetized, and cortical responses to S+ and S- were recorded in the right auditory cortex. When a pure tone (PT) at 10 kHz and a 10 kHz amplitude-modulated (AM) sound were used as S+ and S-, the cortical responses to S using AM were significantly depressed but those to S- using PT were not. The cortical responses to S+ showed no significant change. Upward frequency-modulated sounds from 5 kHz to 40 kHz (FM up arrow) and downward frequency-modulated sounds from 40 kHz to 5 kHz (FM down arrow) were also used as S+ and S. Cortical responses to S- using FM up arrow and FM down arrow were significantly depressed after learning, while those to S+ were unchanged. No significant change of cortical responses to S using FMs was observed in the left auditory cortex after learning. The learning-induced depression of S using FMs was most clearly observed in the medial part of the tonotopic band to 40 kHz in the right primary auditory cortex, which might be involved in processing FM sounds. (C) 2010 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

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  • Transcranial imaging of somatotopic map plasticity after tail cut in mice 査読

    Hiroki Kitaura, Ryuichi Hishida, Katsuei Shibuki

    BRAIN RESEARCH   1319   54 - 59   2010年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Peripheral afferent denervation induces reorganization of somatotopic maps in the primary somatosensory cortex (S1). In the present study, we investigated somatotopic map plasticity after tail cut. Neonatal mice at postnatal days (P) 2-3 and adult mice at eight weeks of age were anesthetized with ether, and approximately two thirds of the tail was cut from the tip. Both groups of mice were anesthetized with urethane (1.7 g/kg, i.p.) at 10 weeks of age, and transcranial flavoprotein fluorescence imaging was performed in the Si. Neural activities in the Si were elicited by vibratory stimulation applied to the contralateral hindpaw or the tail in control mice. The cortical areas activated by hindpaw, tail base, and tail tip stimuli were placed in this order according to the medial and posterior direction. In mice with tail cut, the tail base area moved to the more medial and posterior area corresponding to the tail tip in control mice. The shift of the tail base area was observed in both neonatal and adult tail cut mice, indicating the absence of a critical period before eight weeks. Medial and posterior shift of the tail base area with regard to the bregma was confirmed in tail cut mice. These data suggest that transcranial flavoprotein fluorescence imaging is a useful technique for investigating somatosensory map plasticity in mice. (C) 2010 Elsevier B.V. All rights reserved.

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  • Flavoprotein fluorescence imaging of experience-dependent cortical plasticity in rodents. 査読

    Katsuei Shibuki, Ryuichi Hishida, Manavu Tohmi, Kuniyuki Takahashi, Hiroki Kitaura, Yamato Kubota

    In Vivo Optical Imaging of Brain Function. 2nd edition. (Frostig RD, ed), Chapter 7, Frontiers in Neuroscience   193 - 220   2009年5月

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    記述言語:英語   掲載種別:論文集(書籍)内論文  

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  • Transcranial flavoprotein fluorescence imaging of mouse cortical activity and plasticity 査読

    Manavu Tohmi, Kuniyuki Takahashi, Yamato Kubota, Ryuichi Hishida, Katsuei Shibuki

    JOURNAL OF NEUROCHEMISTRY   109 ( Suppl 1 )   3 - 9   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Endogenous fluorescence signals derived from mitochondria reflect activity-dependent changes in brain metabolism and may be exploited in functional brain imaging. Endogenous flavoprotein fluorescence imaging in mice is especially important because many genetically manipulated strains of mice are available and the transparent skull of mice allows transcranial fluorescence imaging of cortical activities. In the primary sensory areas of mice, cortical activities and experience-dependent plasticity have been investigated using transcranial fluorescence imaging. Furthermore, differential imaging, based on stimulus specificity of cortical areas, distinguished activities in higher visual areas around the primary visual cortex from those in primary visual cortex. The combination of transcranial fluorescence imaging with the suppression of cortical activities using photobleaching of flavoproteins is expected to aid in elucidating the roles of sensory cortices including higher areas in mice.

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  • Transcranial photo-inactivation of neural activities in the mouse auditory cortex 査読

    Yamato Kubota, Daiki Kamatani, Hiroaki Tsukano, Shinsuke Ohshima, Kuniyuki Takahashi, Ryuichi Hishida, Masaharu Kudoh, Sugata Takahashi, Katsuei Shibuki

    NEUROSCIENCE RESEARCH   60 ( 4 )   422 - 430   2008年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Flavoprotein fluorescence in the brain is intimately coupled with neuronal aerobic energy metabolism. If flavoproteins are photobleached, neural activities may be affected owing to dysfunction in aerobic energy metabolism in mitochondria. We tested this possibility in cortical slices from mice, and found that exposure to blue light (lambda = 475 nm) derived from a 20 mW diode laser for 50 min suppresses trans-synaptic components of field potentials. This finding formed the basis of a transcranial photo-inactivation technique, that was used to investigate auditory signal transmission between the anterior auditory field (AAF) and the primary auditory cortex (AI) in anesthetized mice. Cortical responses in AAF and AI, elicited by 5 kHz tonal stimuli, were visualized using transcranial flavoprotein fluorescence imaging. After determining responsive areas in AAF and AI, the auditory cortex was exposed to the blue diode laser via the intact skull, while either AAF or AI was protected with a piece of carbon paper. Although the photo-inactivation of AI had no significant effect on the fluorescence responses in AAF, the photo-inactivation of AAF significantly reduced the fluorescence responses in AI, indicating the presence of auditory signal transmission from AAF to AI. (C) 2008 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

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  • Long-term depression induced by local tetanic stimulation in the rat auditory cortex 査読

    Kenji Watanabe, Daiki Kamatani, Ryuichi Hishida, Masaharu Kudoh, Katsuei Shibuki

    BRAIN RESEARCH   1166   20 - 28   2007年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    In sensory cortices, synaptic plasticities such as long-term potentiation (LTP) and long-term depression (LTD) have important roles in the development of neural circuits and sensory information processing. However, the differential roles and mechanisms of the various types of LTP and LTD are not clear. In the present study, we investigated LTP and two types of LTD in slices obtained from the rat auditory cortex. Supragranular field potentials elicited by layer VI stimulation were recorded through a metal electrode. Transsynaptic field potentials exhibited marked LTP after tetanic stimulation (TS, 100 Hz for 1 s) was applied to layer VI. The same field potential components exhibited LTD after low-frequency stimulation (LFS, 1 Hz for 900 s) was applied to layer VI. LTD of supragranular field potentials was also induced by local TS applied to supragranular layers 0.3 mm from the recording site. Neither LTP nor LTD of either type was induced in the presence of 50 mu M D-(-)-2 -amino-5-phosphonovalerate (APV), an NMDA receptor antagonist. However, 500 mu M (+)-alpha-methyl-4-carboxyphenylglycine (MCPG), an antagonist of metabotropic glutamate receptors, had no effect. LTD induced by LFS and that induced by local TS were suppressed in the presence of 3 mu M bicuculline, an antagonist of GABA(A) receptors. Each of these forms of LTD occluded the other. These results and intracellular recordings in supragranular pyramidal neurons during LFS and local TS strongly suggest that the two types of LTD share common neural circuits for their induction. (c) 2007 Published by Elsevier B.V.

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  • Experience-dependent formation of activity propagation patterns at the somatosensory S1 and S2 boundary in rat cortical slices 査読

    Dalki Kamatani, Ryuichi Hishida, Masaharu Kudoh, Katsuei Shibuki

    NEUROIMAGE   35 ( 1 )   47 - 57   2007年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Somatosensory information is serially processed by the primary (S1) and secondary (S2) cortices, which can be identified in fresh cortical slices. We visualized activity propagation between S1 and S2 in rat cortical slices using flavoprotein fluorescence imaging. When S1 was stimulated, fluorescence responses extended into S2, while responses hardly propagated to S1 following S2 stimulation. The dominant activity propagation pattern from S1 to S2 was not affected by antagonists of glutamate or GABA(A) receptors. Ca2+ imaging and electrophysiological recordings confirmed the anisotropic activity propagation pattern. This pattern could be formed as a result of serial information processing in SI and S2. To test this hypothesis, activity propagation was investigated in cortical slices prepared 2 weeks or 3 days after trimming contralateral whiskers that provide massive inputs to SI. Supragranular activities in the barrel cortex were clearly suppressed. Furthermore, activities elicited in the rostral small vibrissae/mouth area of S1 near the border between S I and S2 spread into the adjacent barrel cortex rather than into S2. Behavioral effects of whisker trimming were evaluated using a test, in which rats chose one of two bridges that had a wall on the right or left side only. Immediately after hemilateral whisker trimming, rats preferred to use the bridge with a wall close to the intact side. However, this preference disappeared 3 days after trimming. Modified activities observed in cortical slices after whisker trimming might be mechanisms for this behavioral compensation. These findings suggest experience-dependent formation of activity propagation patterns in the somatosensory cortex. (c) 2006 Elsevier Inc. All rights reserved.

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  • Functional local connections with differential activity-dependence and critical periods surrounding the primary auditory cortex in rat cerebral slices 査読

    Ryuichi Hishida, Daiki Kamatani, Hiroki Kitaura, Masaharu Kudoh, Katsuei Shibuki

    NEUROIMAGE   34 ( 2 )   679 - 693   2007年1月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Sensory information is processed in neural networks connecting the primary sensory cortices with surrounding higher areas. Here, we investigated the properties of local connections between the primary auditory cortex (area 41) and surrounding areas (areas 20, 36, 18a and 39) in rat cerebral slices. Neural activities elicited by repetitive electrical stimulation were visualized using the activity-dependent changes in endogenous fluorescence derived from mitochondrial flavoproteins, which mostly reflect activities produced by polysynaptic glutamatergic transmission. Polysynaptic feedforward propagation was dominant compared with the corresponding polysynaptic feedback propagation between the primary (area 41) and secondary (areas 20 and 36) auditory cortices, while such a tendency was less clear in other pathways. Long inter-areal (> 1 mm) propagation with the same dominancy was observed after laver V stimulation between areas 41 and 20, and was not affected by cutting the underlying white matter. Activity-dependent changes in neural activities induced by low-frequency stimulation in the presence of I mu M bicuculline were investigated using Ca2+ imaging. Significant potentiation of the polysynaptic Ca2+ activities was only observed in polysynaptic feedforward pathways from the primary to secondary auditory cortices. Experience-dependence of the connections between areas 41 and 20 was investigated using flavoprotein fluorescence imaging. The activities from areas 41 to 20 were reduced by cochlear lesions produced at P12 but not at P28, while the activities from areas 20 to 41 were reduced by the lesions at P28, suggesting the critical period for the polysynaptic feedforward connection was before P28, while for the polysynaptic feedback connection was after P28. (c) 2006 Elsevier Inc. All rights reserved.

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  • Coupling of brain function and metabolism: Endogenous flavoprotein fluorescence imaging of neural activities by local changes in energy metabolism 査読

    K. Shibuki, R. Hishida, H. Kitaura, K. Takahashi, M. Tohmi

    Handbook of Neurochemistry and Molecular Neurobiology: Brain Energetics. Integration of Molecular and Cellular Processes   5   321 - 342   2007年

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer US  

    The close coupling of neuronal activities to glucose and oxygen metabolism is well established. The imaging of activity-dependent changes in the endogenous fluorescence of NADH and mitochondrial flavoproteins provides the basis for many experimental approaches to visualize brain activities based on local changes in cellular energy metabolism. This chapter summarizes the results of the novel experimental applications of flavoprotein fluorescence for imaging local dynamic coupling of the functional activities of brain cells and temporal linkage to different events (such as electrical activity, calcium flux, and redox changes). These points are discussed in comparison with other methods using endogenous signals (such as deoxygenation of hemoglobin or blood flow changes) and with those using exogenous probes that are sensitive to voltage, calcium, or pH. The technical merits of flavoprotein fluorescence imaging for investigating plastic changes in neural activities and visualizing mouse cortical activities through the intact skull are discussed. Flavoprotein fluorescence imaging is an excellent tool for investigating neural plasticity, and may be a complementary method of functional brain imaging that can be used to understand brain functions. © 2007 Springer Science+Business Media, LLC.

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  • Requirement of the auditory association cortex for discrimination of vowel-like sounds in rats 査読

    Masaharu Kudoh, Yoko Nakayama, Ryuichi Hishida, Katsuei Shibuki

    NEUROREPORT   17 ( 17 )   1761 - 1766   2006年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    We investigated the roles of the auditory cortex in discrimination learning of vowel-like sounds consisting of multiple formants. Rats were trained to discriminate between synthetic sounds with four formants. Bilateral electrolytic lesions including the primary auditory cortex and the dorsal auditory association cortex impaired multiformant discrimination, whereas they did not significantly affect discrimination between sounds with a single formant or between pure tones. Local lesions restricted to the dorsal/rostral auditory association cortex were sufficient to attenuate multiformant discrimination learning, and lesions restricted to the primary auditory cortex had no significant effects. These findings indicate that the dorsal/rostral auditory association cortex but not the primary auditory cortex is required for discrimination learning of vowel-like sounds with multiple formants in rats.

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  • Endogenous fluorescence imaging of somatosensory cortical activities after discrimination learning in rats 査読

    K Shibuki, K Ono, R Hishida, M Kudoh

    NEUROIMAGE   30 ( 3 )   735 - 744   2006年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Aerobic energy metabolism in the brain is reflected as changes in the green fluorescence of mitochondrial flavoproteins, and the activity-dependent changes in endogenous fluorescence are applicable for functional brain imaging. To understand the roles of cortical plasticity in discrimination learning, we used flavoprotein fluorescence imaging to visualize changes of neural activities in the rat primary somatosensory cortex (SI) after learning. Rats were trained to discriminate floor vibration at rewarded and unrewarded frequencies. After this discrimination learning was accomplished in 3-5 days, the rats were anesthetized with urethane (1.5 g/kg, i.p.), and neural responses were recorded in SI during flutter stimuli applied to the contralateral hindpaw. The fluorescence responses to the stimuli at unrewarded frequencies were selectively depressed in the trained rats, which had behaviorally neglected unrewarded stimuli. The depression of cortical responses was not observed in the rats trained with rewarded stimuli only. Therefore, the stimulus-specific depression in SI might explain a part of neural mechanisms underlying discrimination behavior. To reproduce the stimulus-specific depression of cortical responses in anesthetized rats, tetanic cortical stimulation was paired with flutter stimulation applied to the hindpaw. Selective depression of fluorescence responses or field potentials in SI was induced by the paired stimulation. Our findings suggest that some intracortical circuits in SI are specifically tuned to and modulated by unrewarded stimuli of a particular frequency while SI neurons are responsive to both of rewarded and unrewarded stimuli. The present results indicate the usefulness of flavoprotein fluorescence imaging for investigating somatosensory cortical plasticity after learning. (c) 2005 Elsevier Inc. All rights reserved.

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  • Transcranial fluorescence imaging of auditory cortical plasticity regulated by acoustic environments in mice 査読

    K Takahashi, R Hishida, Y Kubota, M Kudoh, S Takahashi, K Shibuki

    EUROPEAN JOURNAL OF NEUROSCIENCE   23 ( 5 )   1365 - 1376   2006年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Functional brain imaging using endogenous fluorescence of mitochondrial flavoprotein is useful for investigating mouse cortical activities via the intact skull, which is thin and sufficiently transparent in mice. We applied this method to investigate auditory cortical plasticity regulated by acoustic environments. Normal mice of the C57BL/6 strain, reared in various acoustic environments for at least 4 weeks after birth, were anaesthetized with urethane (1.7 g/kg, i.p.). Auditory cortical images of endogenous green fluorescence in blue light were recorded by a cooled CCD camera via the intact skull. Cortical responses elicited by tonal stimuli (5, 10 and 20 kHz) exhibited mirror-symmetrical tonotopic maps in the primary auditory cortex (AI) and anterior auditory field (AAF). Depression of auditory cortical responses regarding response duration was observed in sound-deprived mice compared with naive mice reared in a normal acoustic environment. When mice were exposed to an environmental tonal stimulus at 10 kHz for more than 4 weeks after birth, the cortical responses were potentiated in a frequency-specific manner in respect to peak amplitude of the responses in AI, but not for the size of the responsive areas. Changes in AAF were less clear than those in AI. To determine the modified synapses by acoustic environments, neural responses in cortical slices were investigated with endogenous fluorescence imaging. The vertical thickness of responsive areas after supragranular electrical stimulation was significantly reduced in the slices obtained from sound-deprived mice. These results suggest that acoustic environments regulate the development of vertical intracortical circuits in the mouse auditory cortex.

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  • Short-term plasticity visualized with flavoprotein autofluorescence in the somatosensory cortex of anaesthetized rats 査読

    H Murakami, D Kamatani, R Hishida, T Takao, M Kudoh, T Kawaguchi, R Tanaka, K Shibuki

    EUROPEAN JOURNAL OF NEUROSCIENCE   19 ( 5 )   1352 - 1360   2004年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING LTD  

    In the present study, short-term plasticity of somatosensory neural responses was investigated using flavoprotein autofluorescence imaging in rats anaesthetized with urethane (1.5 g/kg, i.p.) Somatosensory neural activity was elicited by vibratory skin stimulation (50 Hz for 1 s) applied on the surface of the left plantar hindpaw. Changes in green autofluorescence (lambda = 500-550 nm) in blue light (lambda = 450-490 nm) were elicited in the right somatosensory cortex. The normalised maximal fluorescence responses (DeltaF/F) was 2.0 +/- 0.1% (n = 40). After tetanic cortical stimulation (TS), applied at a depth of 1.5-2.0 mm from the cortical surface, the responses elicited by peripheral stimulation were significantly potentiated in both peak amplitude and size of the responsive area (both P < 0.02; Wilcoxon signed rank test). This potentiation was clearly observed in the recording session started 5 min after the cessation of TS, and returned to the control level within 30 min. However, depression of the responses was observed after TS applied at a depth of 0.5 mm. TS-induced changes in supragranular field potentials in cortical slices showed a similar dependence on the depth of the stimulated sites. When TS was applied on the ipsilateral somatosensory cortex, marked potentiation of the ipsilateral responses and slight potentiation of the contralateral responses to peripheral stimulation were observed after TS, suggesting the involvement of commissural fibers in the changes in the somatosensory brain maps. The present study clearly demonstrates that functional brain imaging using flavoprotein autofluorescence is a useful technique for investigating neural plasticity in vivo.

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  • Activity-dependent persisting modification of polysynaptic neural circuits involving layer V pyramidal neurons in rat auditory cortex in vitro 査読

    H Kitaura, R Hishida, M Kudoh, K Shibuki

    EUROPEAN JOURNAL OF NEUROSCIENCE   19 ( 2 )   356 - 364   2004年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING LTD  

    Synaptic plasticity in polysynaptic neural circuits permits modulation of the dynamic properties of these circuits. We investigated the properties of polysynaptic potentiation in pyramidal neurons in layer V of rat auditory cortex (AC) slices using the perforated patch clamp technique. The GABA(A) receptor inhibitor bicuculline was used to facilitate polysynaptic activity. The amplitude and duration of the polysynaptic activity were both gradually potentiated with repetitive stimulation (RS) at 12 s intervals. Potentiation was saturated within 10 min of the onset of RS. After the cessation of RS, the polysynaptic responses returned to control levels within 30 min. RS-induced potentiation was confirmed by fluorescence imaging of slices loaded with the Ca2+ indicator rhod-2. Such potentiation was not induced by stimulation at 60 s intervals. The magnitude of the RS-induced potentiation in layer V pyramidal neurons in the AC was greater than that in either layer II/III pyramidal neurons in the AC or layer V pyramidal neurons in the visual cortex. The NMDA receptor antagonist APV (100 mum), inhibited RS-induced potentiation. When stimulated at 1 Hz, the potentiated response appeared rapidly. In the absence of bicuculline, RS consisting of five pulses at 30 ms intervals, repeated at 12 s intervals for 10 min, elicited potentiation of firing activity, suggesting that the potentiation is independent of bicuculline. The present study demonstrates the dynamic properties of polysynaptic circuits involving layer V pyramidal neurons in the AC are strongly affected by activity-dependent synaptic potentiation.

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  • Dynamic imaging of somatosensory cortical activity in the rat visualized by flavoprotein autofluorescence 査読

    K Shibuki, R Hishida, H Murakami, M Kudoh, T Kawaguchi, M Watanabe, S Watanabe, T Kouuchi, R Tanaka

    JOURNAL OF PHYSIOLOGY-LONDON   549 ( 3 )   919 - 927   2003年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CAMBRIDGE UNIV PRESS  

    We used autofluorescence of mitochondrial flavoproteins to image cortical neural activity in the rat. Green autofluorescence in blue light was examined in slices obtained from rat cerebral cortex. About half of the basal autofluorescence was modulated by the presence or absence of 0, or glucose in the medium. Repetitive electrical stimulation at 20 Hz for 1 s produced a localized fluorescence increase in the slices. The amplitude of the increase was 27 +/- 2 % (mean +/- S.D., n = 35). Tetrodotoxin or diphenyleneiodonium, an inhibitor of flavoproteins, blocked the autofluorescence responses. The autofluorescence responses were not observed in slices perfused with calcium-, glucose- or O-2-free medium. In the primary somatosensory cortex of rats anaesthetized with urethane (1.5 g kg(-1), i.p.), an activity-dependent increase in autofluorescence of 20 +/- 4 % (n = 6) was observed after electrical cortical stimulation at 100 Hz for 1 s, and an increase of 2.6 +/- 0.5 % (n = 33) after vibratory skin stimulation at 50 Hz for 1 s applied to the plantar hindpaw. These responses were large enough to allow visualization of the neural activity without having to average a number of trials. The distribution of the fluorescence responses after electrical or vibratory skin stimulation was comparable to that of the cortical field potentials in the same rats. The fluorescence responses were followed by an increase in arterial blood flow. The former were resistant to an inhibitor of nitric oxide synthase, while the latter was inhibited. Thus, activity-dependent changes in the autofluorescence of flavoproteins are useful for functional brain imaging in vivo.

    DOI: 10.1113/jphysiol.2003.040709

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  • Anisotropic functional connections between the auditory cortex and area 18a in rat cerebral slices 査読

    R Hishida, K Hoshino, M Kudoh, M Norita, K Shibuki

    NEUROSCIENCE RESEARCH   46 ( 2 )   171 - 182   2003年6月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    We developed a new method to visualize the myeloarchitecture in fresh slices, and investigated the properties of the functional neural connections around the boundary between the primary auditory cortex (area 41) and area 18a in rat cerebral slices. A fresh slice illuminated by near-vertical light was observed with a CCD camera. The translucent images of the slice showed contrast patterns very similar to mycloarchitecture. The boundary between these areas was identified by the well-developed layer IV/V in area 41 but not in area 18a. Antidromic/presynaptic components of the field potentials stimulated and recorded across the areal boundary showed symmetric distribution, while the postsynaptic field potentials in the direction from area 41 to 18a were more prominent than those in the opposite direction in layer II/III. In contrast, the dominant direction of propagation of postsynaptic potentials was from area 18a to 41 in layer V. In the presence of 1 muM bicuculline, an inhibitor of GABA(A) receptors, the polysynaptic activities propagating from area 18a into 41 via layer V were elicited by stimulation of area 18a. The propagation measured by Ca2+ imaging or field potential recordings was potentiated after both areas 18a and 41 were alternately stimulated several times. (C) 2003 Elsevier Science Ireland Ltd and Japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/S0168-0102(03)00059-2

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  • hch-1, a gene required for normal hatching and normal migration of a neuroblast in C-elegans, encodes a protein related to TOLLOID and BMP-1 査読

    R Hishida, T Ishihara, K Kondo, Katsura, I

    EMBO JOURNAL   15 ( 16 )   4111 - 4122   1996年8月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS UNITED KINGDOM  

    Proteins of the tolloid/bone morphogenetic protein (BMP)-1 family play important roles in the differentiation of cell fates, Among those proteins are BMP-1, which plays a role in cartilage and bone formation in mammals, the TOLLOID protein, which is required for the establishment of the dorsoventral axis of Drosophila embryos and BP10/SpAN, which are thought to act in the morphogenesis of sea urchins, These proteins have some properties in common, First, they contain the astacin metalloprotease domain, the CUB domain and the epidermal growth factor-like domain, Second, they are expressed in embryos at stages expected for their role in cell differentiation. Third, at least BMP-1 and TOLLOID are thought to interact with proteins of the transforming growth factor-beta family. We report that the hch-1 gene of the nematode Caenorhabditis elegans encodes a tolloid/BMP-1 family protein, The protein has the characteristic domains common to the tolloid/BMP-1 family, Like other members of the family, it is expressed in embryos, However, the phenotype of hch-1 mutants shows that it is required for normal hatching and normal migration of a post-embryonic neuroblast. Furthermore, in spite of its expression in embryogenesis, it is not required for the viability of embryos, These results show new functions of the tolloid/BMP-1 family proteins and give insight into their evolution.

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  • THE ROLE OF ACIDIC RESIDUES IN THE FUSION SEGMENT OF INFLUENZA-A VIRUS HEMAGGLUTININ IN LOW-PH-DEPENDENT MEMBRANE-FUSION 査読

    E NOBUSAWA, R HISHIDA, M MURATA, K KAWASAKI, S OHNISHI, K NAKAJIMA

    ARCHIVES OF VIROLOGY   140 ( 5 )   865 - 875   1995年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG WIEN  

    To clarify the role of acidic amino acid residues in the ''fusion segment'' of hemagglutinin (HA) of influenza A virus (H1N1) in pH-dependent membrane fusion, we have constructed and expressed five mutant HA cDNAs in CV-1 cells by SV40-HA virus vectors (SVHA). Fusion activities of the five mutant HAs were examined by lipid mixing and polykaryon formation assays. In spite of the substitution of Gly and Lys for the acidic residues, all the mutants were found to retain their low-pH-dependent fusion activity by lipid mixing assay. Although SVHA-G19(HA(2)19D-->G), -K11(HA(2)11E-->K) and -K19(HA(2)19D-->K) induced polykaryon formation at low pH as wild type HA did, SVHA-G11(HA(2)11E-->G) induced limited polykaryon formation and SVHA-G11,19 (HA(2)11E-->G, 19D-->G) did not. The substitution of Gly for Glu at position 11 inhibited widening of the initial fusion pore. However, Lys mutants induced the formation of an initial fusion pore and widened it at low pH where Lys residues might have positive charges. These results suggest that the neutralization of the charges on acidic residues in the ''fusion segment'' at low pH is not important for interaction of the ''fusion segment'' with the target lipid bilayer or for triggering the membrane fusion.

    DOI: 10.1007/BF01314963

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  • SPECIFICITY OF AMPHIPHILIC ANIONIC PEPTIDES FOR FUSION OF PHOSPHOLIPID-VESICLES 査読

    M MURATA, S TAKAHASHI, Y SHIRAI, S KAGIWADA, R HISHIDA, S OHNISHI

    BIOPHYSICAL JOURNAL   64 ( 3 )   724 - 734   1993年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOPHYSICAL SOCIETY  

    We have synthesized five amphiphilic anionic peptides derived from E5 peptide [Murata, M., Takahashi, S., Kagiwada, S., Suzuki, A., Ohnishi, S. 1992. Biochemistry 31:1986-1992. E5NN and E5CC are duplications of the N-terminal and the C-terminal halves of E5, respectively, and E5CN is an inversion of the N- and the C-terminal halves. E5P contains a Pro residue in the center of E5 and E8 has 8 Glu residues and 9 Leu residues, We studied fusion of dioleoylphosphatidylcholine (DOPC) large unilamellar vesicles assayed by fluorescent probes. The peptides formed alpha-helical structure with different degrees; E5NN, E5CN, and E8 with high helical content and E5CC and E5P with low helical content. These peptides bound to DOPC vesicles at acidic pH in proportion to the helical content of peptide. The peptides caused leakage of DOPC vesicles which increased with decreasing pH. The leakage was also proportional to the helicity of peptide. Highly helical peptides E5NN, E5CN, and E8 caused hemolysis at acidic pH but not at neutral pH. The fusion activity was also dependent on the helicity of peptides. In fusion induced by an equimolar mixture of E5 analogues and K5 at neutral pH, E8, E5NN, and E5CN were most active but E5CC did not cause fusion. In fusion induced by E5-analogue peptides alone, E5CN was active at acidic pH but not at neutral pH. Other peptides did not cause fusion. Amphiphilic peptides also appear to require other factors to cause fusion.

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  • INVITRO FUSION OF RABBIT LIVER GOLGI MEMBRANES WITH LIPOSOMES 査読

    S KAGIWADA, M MURATA, R HISHIDA, M TAGAYA, S YAMASHINA, S OHNISHI

    JOURNAL OF BIOLOGICAL CHEMISTRY   268 ( 2 )   1430 - 1435   1993年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Fusion of Golgi membranes isolated from rabbit liver with liposomes was studied by lipid mixing of fluorescent lipid analogues and internal content mixing and by electron microscopic observation of transfer of horseradish peroxidase from liposomes into Golgi membranes. A monoclonal antibody was used to confirm fusion of Golgi membranes but not other contaminating vesicles. Fusion was rapid and efficient, reaching about 20% of the maximum after a 5-min incubation using small or large unilamellar dioleoylphosphatidylcholine vesicles. The fusion was dependent on temperature, decreasing at lower temperatures, and becoming nearly zero below 10-degrees-C. The addition of ATP, GTP, cytosolic factors, or N-ethylmaleimide did not affect fusion. Treatments of Golgi membranes with 0.1 M Na2CO3 or 1 M KCl did not cause any changes in fusion. However, treatment with proteases inhibited fusion. These results suggest that Golgi integral membrane protein(s) are involved in fusion. Changing the medium to an isoosmotic substance, sucrose, in place of KCl or NaCl inhibited fusion. The binding assay of fluorescent liposomes to Golgi membranes showed that lowering the temperature or replacing salts with sucrose did not affect binding. However, treatment of Golgi membranes with proteases inhibited binding. Addition of phosphatidylserine or phosphatidylethanolamine to dioleoylphosphatidylcholine liposomes caused a 2-fold increase in binding and fusion. Fusion between Golgi membranes by themselves did not occur. These results provide some information on the mechanism of intracellular vesicular transport.

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  • INTERACTION OF THE GOLGI MEMBRANES ISOLATED FROM RABBIT LIVER WITH MICROTUBULES INVITRO 査読

    M MURATA, TJ ITOH, S KAGIWADA, R HISHIDA, H HOTANI, S OHNISHI

    BIOLOGY OF THE CELL   75 ( 2 )   127 - 134   1992年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:EDITIONS SCIENTIFIQUES ELSEVIER  

    We have developed a reconstituted model system to study the interaction of the Golgi membranes isolated from rabbit liver with taxol-stabilized bovine-brain microtubules without microtubule-associated proteins (MAPs). The Golgi membranes are associated with microtubules. The sheets of vesicles and the membranous tubules are observed along microtubules by direct visualization using differential-interference-contrast, dark field, or fluorescence microscopy. The monoclonal antibody against Golgi membranes suggests that the Golgi membranes, but not the contaminating vesicles, are interacting with microtubules. The degree of association is assayed quantitatively using rhodamine-labeled microtubules after separation of the complex from unbound microtubules by centrifugation upon sucrose gradient, The association is inhibited by crude MAPs, purified MAP2, or 1.0 mM ATP. However, the association neither requires the cytosol from rat liver or bovine brain nor N-ethylmaleimide, brefeldin A, or GTP-gamma-S. The association is mediated by trypsin-sensitive peripheral protein(s) on the Golgi membranes.

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  • MODIFICATION OF THE N-TERMINUS OF MEMBRANE FUSION-ACTIVE PEPTIDES BLOCKS THE FUSION ACTIVITY 査読

    M MURATA, S KAGIWADA, R HISHIDA, R ISHIGURO, S OHNISHI, S TAKAHASHI

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   179 ( 2 )   1050 - 1055   1991年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1016/0006-291X(91)91925-3

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MISC

  • マウス大脳皮質感覚野の経験による修飾

    澁木克栄, 吉武講平, 駒形成司, 塚野浩明, 大島伸介, 渡邉健児, 任海学, 菱田竜一

    生体の科学   60   75 - 80   2009年

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    記述言語:日本語  

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  • 感覚野経験依存的可塑性の経頭蓋蛍光イメージング

    澁木克栄, 駒形成司, 吉武講平, 塚野浩明, 菱田竜一

    蛋白質 核酸 酵素 増刊号   53   512 - 517   2008年

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    記述言語:日本語  

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  • Flavoprotein autofluorescence.

    Katsuei Shibuki, Ryuichi Hishida

    Physiology News   54   18 - 19   2004年12月

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  • 聴覚野神経活動の内因性酸化還元蛍光によるイメージング 招待

    菱田竜一, 工藤雅治, 澁木克栄

    日本生理学雑誌   64   15 - 17   2002年12月

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    担当区分:筆頭著者   記述言語:日本語  

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共同研究・競争的資金等の研究

  • 痛覚刺激による注意干渉の回路メカニズム:マウス皮質イメージングを用いた解析

    研究課題/領域番号:18K06518  2018年4月 - 2022年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    菱田 竜一

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    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

    1、注意緩衝領域として同定したマウス頭頂連合野と、視覚のコントラスト感受性に関する解析を麻酔下フラビンイメージングによって解析した。頭頂連合野から一次視覚野への抑制投射経路を皮質切断により遮断したマウスに異なるコントラストの視覚刺激を与え、視覚野応答を切断前後で比較した。興味深いことに、高コントラストの視覚刺激に対しては皮質の視覚応答が増強する一方、逆に低コントラストの視覚刺激に対しては抑制されるという現象を見いだし、頭頂連合野が視覚野の興奮・抑制バランスの制御に関与しているとの示唆を得た。この結果は、刺激強度の強い感覚刺激に対しては過剰な皮質応答を押さえ、強度の弱い刺激に対しては応答を底上げし、皮質回路において適切なダイナミックレンジ内に感覚応答を納めるべく頭頂連合野が機能していると解釈される。
    2、2光子顕微鏡によるカルシーウムイメージングの結果を解析し、頭頂連合野が視覚野の細胞応答に及ぼす効果を調べた。4方位・8方向の線分刺激をマウスに提示し、一次視覚野の興奮性細胞および抑制性細胞が示す視覚応答を、コントロールマウス、頭頂連合野から一次視覚野への抑制投射経路を皮質切断により遮断した直後のマウス(急性遮断マウス)、遮断から3週間以上の回復期を経たマウス(慢性遮断マウス)の3群で比較した。方向選択性に対する効果を解析したところ、急性および慢性遮断マウスにおいて、方向選択性の低下が見られた。その時間的経過を解析したところ、急性遮断マウスと慢性遮断マウスで差異が見られた。急性遮断マウスでは、興奮の極初期での抑制が解除され、視覚野興奮性細胞の興奮性そのものが亢進していると解釈できた。一方、慢性遮断マウスでは、興奮の後半期での抑制が解除されており、方向選択性の生成メカニズムに抑制投射経路が関与しているとの示唆を得た。

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  • 注意の選択フィルターを形成するマウス頭頂連合野の機能解析

    研究課題/領域番号:26430011  2014年4月 - 2018年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    菱田 竜一

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    配分額:5070000円 ( 直接経費:3900000円 、 間接経費:1170000円 )

    マウス頭頂連合野から一次視覚野への逆行性投射が存在し、一次視覚野を抑制することを見出したが、その生理学的役割は不明であった。そこで、「頭頂連合野からの投射が一次視覚野に選択的注意の初期選択フィルターを形成しているのでは?」という仮説を立て解析を進めたところ、①一次視覚野抑制の可塑性は、視覚刺激への特異性を有していること、②一次視覚野興奮性細胞の方位選択性を修飾すること、③抑制機能は頭頂連合野にマッピングされた、等を明らかにした。これらの結果は、仮説を支持するものと考えられ、さらに踏み込んだ解析を勇気づけるものであった。

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  • マウス帯状回による注意の神経回路メカニズムの解明

    研究課題/領域番号:23500383  2011年 - 2013年

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    菱田 竜一

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    配分額:5200000円 ( 直接経費:4000000円 、 間接経費:1200000円 )

    高次領野からの逆行性投射は、皮質の感覚機能を修飾するとされるが、その詳しい機序は不明である。そのメカニズムを解明するため、マウス高次領野から一次視覚野への抑制性投射を経頭蓋フラビン蛋白質イメージングにより解析した。トレーサー実験によれば、その抑制性投射は直接経路と間接経路により構成されていることが示唆された。阻害実験によりその投射が、一次視覚野を常時抑制していること、視覚のコントラスト感受性に関与することが明らかにした。

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  • 逐次経頭蓋電気刺激法によるマウス頭頂連合野の機能解析

    2008年4月 - 2010年3月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    菱田 竜一

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    資金種別:競争的資金

    新たに開発した逐次経頭蓋電気刺激法による、マウス大脳皮質での感覚情報伝達経路の探索および解析。主に、視覚・聴覚・体性感覚という3つの異なるモダリティの感覚情報が統合される領野の探索。

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  • ラット視覚野・聴覚野に隣接する高次領野を介した視聴覚連合機構の解析

    2002年4月 - 2005年3月

    日本学術振興会  科学研究費助成事業  若手研究(B)

    菱田 竜一

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    資金種別:競争的資金

    配分額:2400000円 ( 直接経費:2400000円 )

    異なる領野間の機能的結合の特性と可塑性を解析した。解析対象は、41野(1次聴覚野)とその周辺の高次感覚野、20野(2次聴覚野)・36野(2次聴覚野)・18a野(2次視覚野)・39野(2次体性感覚野)である。スライス切断面を調整することで、41野といずれかの高次感覚野を含む脳スライスを調整、解析して次の4つの結果を得た。
    (1)領野間の機能的結合は、場所と方向により性質が異なる。
    (2)領野間の長距離機能的結合には、白質を介さず、灰白質内だけを通るものもある。
    (3)領野間の機能的結合には、反復刺激により増強するものがある。
    (4)領野間の機能的結合の可塑性には、固有の感受性期がある。

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担当経験のある授業科目(researchmap)

  • 脳神経学

    機関名:新潟医療技術専門学校

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  • 生理機能検査学

    機関名:新潟医療技術専門学校

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  • 解剖生理学実験

    機関名:悠久山栄養調理専門学校

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  • 医学研究実習

    機関名:新潟大学

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  • 医科学研究法

    機関名:新潟大学

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  • 生理学

    機関名:新潟大学

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