Updated on 2024/05/26

写真a

 
MATSUNO Yoko
 
Organization
University Medical and Dental Hospital Medical Laboratory Division Lecturer
Graduate School of Medical and Dental Sciences Community Disease Control Lecturer
Title
Lecturer
External link

Degree

  • 博士(医学) ( 1990.7   山口大学 )

Research Areas

  • Life Science / Genome biology

Research History

  • Niigata University   University Medical and Dental Hospital Medical Laboratory Division   Lecturer

    2008.9

  • Niigata University   Graduate School of Medical and Dental Sciences Community Disease Control   Lecturer

    2008.9

  • Niigata University   Graduate School of Medical and Dental Sciences Biomedical Sciences   Lecturer

    2008.9

 

Papers

  • Versatility of RNA-Binding Proteins in Living Cells Reviewed

    Riki Kurokawa, Akihide Takeuchi, Nobuyuki Shiina, Masato Katahira, Takefumi Yamashita, Yoko Matsuno

    Biomedical Sciences   5 ( 1 )   7 - 13   2019.8

     More details

    Language:English   Publishing type:Research paper (conference, symposium, etc.)  

    DOI: 10.11648/j.bs.20190501.12

    researchmap

  • Convergence in LINE-1 nucleotide variations can benefit redundantly forming triplexes with lncRNA in mammalian X-chromosome inactivation Reviewed

    Yoko Matsuno, Takefumi Yamashita, Michiru Wagatsuma, Hajime Yamakage

    Mobile DNA   13 ( 1 )   2019.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1186/s13100-019-0173-4

    researchmap

    Other Link: http://link.springer.com/article/10.1186/s13100-019-0173-4/fulltext.html

  • Multiplicity in long noncoding RNA in living cells Reviewed

    Kurokawa R, Komiya R, Oyoshi T, Matsuno Y

    Biomedical Sciences   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    researchmap

  • X-inactivation in female human embryonic stem cells is in a nonrandom pattern and prone to epigenetic alterations Reviewed

    Yin Shen, Youko Matsuno, Shaun D. Fouse, Nagesh Rao, Sierra Root, Renhe Xu, Matteo Pellegrini, Arthur D. Riggs, Guoping Fan

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   105 ( 12 )   4709 - 4714   2008.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATL ACAD SCIENCES  

    X chromosome inactivation (XCI) is an essential mechanism for dosage compensation of X-linked genes in female cells. We report that subcultures from lines of female human embryonic stem cells (hESCs) exhibit variation (0-100%) for XCI markers, including XIST RNA expression and enrichment of histone H3 lysine 27 trimethylation (H3K27me3) on the inactive X chromosome (Xi). Surprisingly, regardless of the presence or absence of XCI markers in different cultures, all female hESCs we examined (H7, H9, and HSF6 cells) exhibit a monoallelic expression pattern for a majority of X-linked genes. Our results suggest that these established female hESCs have already completed XCI during the process of derivation and/or propagation, and the XCI pattern of lines we investigated is already not random. Moreover, XIST gene expression in subsets of cultured female hESCs is unstable and subject to stable epigenetic silencing by DNA methylation. In the absence of XIST expression, approximate to 12% of X-linked promoter CpG islands become hypomethylated and a portion of X-linked alleles on the Xi are reactivated. Because alterations in dosage compensation of X-linked genes could impair somatic cell function, we propose that XCI status should be routinely checked in subcultures of female hESCs, with cultures displaying XCI markers better suited for use in regenerative medicine.

    DOI: 10.1073/pnas.0712018105

    Web of Science

    researchmap

  • Inhibition of Atm and/or Atr disrupts gene silencing on the inactive X chromosome Reviewed

    OY Yan, J Salstrom, S Diaz-Perez, S Nahas, Y Matsuno, D Dawson, MA Teitell, S Horvath, AD Riggs, RA Gatti, Y Marahrens

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   337 ( 3 )   875 - 880   2005.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    ATM and ATR are well documented for their roles in maintaining the integrity of genomic DNA by responding to DNA damage and preparing the cell for repair. Since ATM and ATR have been reported to exist in complexes with historic deacetylases, we asked whether Atm and Atr might also uphold gene silencing by heterochromatin. We show that the Atm/Atr inhibitor 2-aminopurine causes the inactive X chromosome to accumulate abnormal chromatin and undergo unwanted gene reactivation. We provide evidence that this gene expression from the inactive X chromosome is not a byproduct of the accumulation of DNA breaks. Individually inhibiting Atm and Atr by either small interfering RNA or the expression of dominant-negative ATM and ATR constructs also compromised X-inactivation. Atm and Atr, therefore, not only function in responding to DNA damage but perhaps also are involved in gene silencing via the maintenance of heterochromatin. (c) 2005 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2005.09.122

    Web of Science

    researchmap

  • 診断検査についてのEvidenceの収集を目指したWebベースシステムの構築 Reviewed

    石田博, 北村聖, 三宅一徳, 西堀眞弘, 松野容子, 井上裕二

    医療情報学   24   89 - 97   2004

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • 救命センター内ICUへの自動血液培養装置導入の背景 Reviewed

    山下 進, 松野容子, 鶴田良介

    日本集中治療医学会雑誌   2001

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • NO2 and No3 Measurement for the detection of Escherichia coli in blood samples Reviewed

    Tsuruta R, Matsuno Y, Mizuno H, Sadamitsu D, Maekawa T

    The Bulletin of the Yamaguchi Medical school   46 ( 3-4 )   93 - 97   2000

     More details

    Language:English  

    researchmap

  • 臨床検査データベースの有効利用 付加価値に対する検査室の今後の取り組み Reviewed

    松野容子, 井上祐二, 水野秀一, 大場雄三

    臨床病理   47   501 - 508   1999

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • 使い易い蛋白分画の提示法 ~日常診療への有効利用を目指して~ Reviewed

    松野容子, 藤井俊子, 三浦啓子, 敷地恭子, 田中浩

    日本臨床化学会四国支部会誌   16   33 - 44   1999

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • 敗血症に対する検査室の今後の取り組み Reviewed

    松野容子, 鶴田良介, 坂部武史

    臨床病理   47   501 - 508   1999

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • QC手法を用いた輸血当直業務に関する現状解析 Reviewed

    丸田雄一, 境一, 中野かおり, 敷地恭子, 水野秀一, 江角智子, 亀岡満子, 松野容子

    医学検査   47   916 - 922   1998

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • 微生物検査情報の品質管理と院内感染防止対策への活用 Reviewed

    松野容子, 井上裕二, 水野秀一, 大庭雄三

    臨床病理   46   1097 - 1102   1998

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • Helicobacter pylori (H. pylori) の感染診断: 培養法, Rapid Urease Test, 鏡検法および抗H. pylori Ig-G抗体法の比較 Reviewed

    中村弘毅, 柳井秀雄, 西川潤, 時山裕, 吉田智治, 多田正弘, 沖田極, 松野容子, 高橋睦夫, 星井嘉信

    山口医学   46   231 - 236   1997

     More details

    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

    researchmap

  • 抗酸菌遺伝子検査に関する意識調査と院内導入後の問題点 Reviewed

    松野容子, 水野秀一, 常岡えい子, 大庭雄三

    臨床病理   45   141 - 147   1997

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • 病院エレベーターの押しボタンと手指を対象とした菌の伝播に関する一考察 Reviewed

    松野容子, 水野秀一, 大徳優子

    環境感染   11   123 - 127   1996

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • 菌の生息場所としての病院環境と院内感染対策について Reviewed

    松野

    Laboratory and Clinical Practice   12   142 - 143   1994

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • A POSSIBLE EXAMPLE OF GENE CONVERSION WITH A COMMON BETA-THALASSEMIA MUTATION AND CHI-SEQUENCE PRESENT IN THE BETA-GLOBIN GENE Reviewed

    Y MATSUNO, Y YAMASHIRO, K YAMAMOTO, Y HATTORI, K YAMAMOTO, Y OHBA, T MIYAJI

    HUMAN GENETICS   88 ( 3 )   357 - 358   1992.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER VERLAG  

    Web of Science

    researchmap

  • 3 BETA-THALASSEMIA MUTATIONS IN THE JAPANESE - IVS-II-1 (G-]A), IVS-II-848 (C-]G), AND CODON-90 (GAG-]TAG) Reviewed

    Y HATTORI, K YAMAMOTO, Y YAMASHIRO, Y OHBA, S MIYAMURA, K YAMAMOTO, Y MATSUNO, M MORISHITA, T MIYAJI, T ERA

    HEMOGLOBIN   16 ( 1-2 )   93 - 97   1992

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MARCEL DEKKER INC  

    Web of Science

    researchmap

  • Legionella Pneumoniaの1剖検例 Reviewed

    河村俊治, 松野容子

    病院病理   10   124 - 124   1992

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • LPIAによるFDP-E測定の尿検体への応用 Reviewed

    松野容子, 米原ヤス子, 宮地隆興

    医学と薬学   23   613 - 618   1990

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • Prolonged neuromuscular and cardiovascular effects of succinylcholine in a patient homologous for the silent gene Reviewed

    Yamamoto M, Wakuta K, Oshita S, Hiraoka I, Takeshita, Matsuno Y, Maekawa T

    The Balletin of the Yamaguchi Medical School   37 ( 3-4 )   119 - 122   1990

     More details

    Language:English   Publisher:Yamaguchi University Graduate School of Medicine  

    CiNii Article

    CiNii Books

    researchmap

    Other Link: http://search.jamas.or.jp/link/ui/1992141629

  • 3 JAPANESE FAMILIES WITH HB H-DISEASE - GENE ANALYSES AND THEIR CHARACTERIZATIONS Reviewed

    Y HATTORI, M MORISHITA, Y YAMASHIRO, K YAMAMOTO, K YAMAMOTO, Y MATSUNO, Y OHBA, T MIYAJI

    HEMOGLOBIN   14 ( 5 )   559 - 567   1990

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MARCEL DEKKER INC  

    Web of Science

    researchmap

  • 日本人に新しく見出されたβ0サラミセア遺伝子 : コドン41-42における4塩基欠失変異 Reviewed

    松野容子

    山口医学   39   203 - 215   1990

     More details

    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

    researchmap

  • 日本人に見出された遺伝性HbH病 1家系とその遺伝子分析 Reviewed

    服部幸夫, 山城 安啓, 山本邦光, 森下 待子, 宮地 隆興, 山本きよみ, 松野容子

    臨床血液   31 ( 2 )   183 - 188   1990

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:(一社)日本血液学会-東京事務局  

    researchmap

  • プローブを用いた日本人βサラセミアの簡単な遺伝子診断 Reviewed

    服部幸夫, 山城安啓, 山本きよみ, 森下待子, 山本邦光, 松野容子

    臨床病理   1989

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • ANOTHER EXAMPLE OF JAPANESE BETA-THALASSEMIA [-31 CAP (A-]G)] Reviewed

    Y YAMASHIRO, Y HATTORI, Y MATSUNO, Y OHBA, T MIYAJI, K YAMAMOTO, K YAMAMOTO, Y NAKAYAMA, Y ABE

    HEMOGLOBIN   13 ( 7-8 )   761 - 767   1989

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MARCEL DEKKER INC  

    Web of Science

    researchmap

  • CHARACTERIZATION OF BETA-THALASSEMIA MUTATIONS AMONG THE JAPANESE Reviewed

    Y HATTORI, A YAMANE, Y YAMASHIRO, Y MATSUNO, K YAMAMOTO, K YAMAMOTO, Y OHBA, T MIYAJI

    HEMOGLOBIN   13 ( 7-8 )   657 - 670   1989

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MARCEL DEKKER INC  

    Web of Science

    researchmap

  • 血清ALP-Ig陽性者の末梢血リンパ球サブセットの解析 Reviewed

    松野容子, 服部幸夫, 山本邦夫

    医学と生物   116   149 - 152   1988

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • 自己免疫性溶血性貧血(AIHA)に合併したALP(アルカリフォスファターゼ)-IgGの一例 Reviewed

    服部幸夫, 山本邦光, 松野容子

    と   115   229 - 301   1987

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • A BETA+-THALASSEMIA (CODON-24, GGT-]GGA) FOUND IN A JAPANESE Reviewed

    Y HATTORI, Y MATSUNO, Y YAMASHIRO, Y OHBA, K YAMAMOTO, K YAMAMOTO, T MIYAJI

    HEMOGLOBIN   11 ( 6 )   644 - 644   1987

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MARCEL DEKKER INC  

    Web of Science

    researchmap

▶ display all

Books

  • 臨床検査薬情報担当者 研修テキストⅡ 血液学検査

    松野容子( Role: Joint author)

    森三樹雄監修 日本臨床検査薬協会編 薬事日報社  2018 

     More details

    Language:Japanese Book type:Textbook, survey, introduction

    researchmap

  • 臨床検査薬情報担当者 研修テキストⅡ 一般検査

    松野容子( Role: Joint author)

    森三樹雄監修 日本臨床検査薬協会編 薬事日報社  2018 

     More details

    Language:Japanese Book type:Scholarly book

    researchmap

  • 臨床検査薬情報担当者 研修テキストⅡ

    松野容子( Role: Sole author)

    森三樹雄監修 日本臨床検査薬協会編 薬事日報社  2012 

     More details

    Responsible for pages:p115-123   Language:Japanese Book type:Textbook, survey, introduction

    researchmap

  • 臨床検査薬情報担当者 研修テキストⅡ 改訂版

    松野容子( Role: Sole author)

    河合忠監修 日本臨床検査薬協会編 薬事日報社  2006 

     More details

    Responsible for pages:p1-24   Language:Japanese Book type:Textbook, survey, introduction

    researchmap

  • 発熱した時に受ける検査

    松野容子( Role: Sole author)

    河野均也監修 日本衛生検査所協会  2001 

     More details

    Responsible for pages:p112-117   Language:Japanese Book type:Scholarly book

    researchmap

  • 臨床検査薬情報担当者 研修テキストⅡ

    松野容子( Role: Joint author)

    河合忠監修 日本臨床検査薬協会編 薬事日報社  1998 

     More details

    Responsible for pages:p1-19   Language:Japanese Book type:Textbook, survey, introduction

    researchmap

  • Pseudomonas aeruginosa による肺炎

    水野秀一, 松野容子( Role: Joint author)

    医歯薬出版  1996 

     More details

    Responsible for pages:98-101   Language:Japanese Book type:Scholarly book

    researchmap

▶ display all

MISC

  • 血液・生化学検査 血液生化学検査に及ぼす因子 Invited

    松野容子

    Journal of Otolaryngology, Head and Neck Surgery   29 ( 9 )   1381 - 1387   2013

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    researchmap

  • 多峰性M蛋白に関する症例 Invited

    松野

    電気泳動の自動化に関するオープンセミナーQ&A集(電気泳動自動化研究会編)   161 - 162   2009

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (other)  

    researchmap

  • 腸管出血性大腸菌感染症 Invited

    松野容子

    Medical Technology   27   1391 - 1394   1999

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    researchmap

  • Biclonal gammopathy Invited

    松野容子

    Progress in AES '98   88 - 89   1998

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (other)  

    researchmap

  • リウマチ(SLE,膠原病)と蛋白分画との関係 Invited

    松野容子, 田中浩

    検査と技術   26   920 - 923   1998

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    researchmap

  • Comparison of Culture Studies, Rapid Urease Tests, Histological Studies and Helicobacter pylori-specific IgG Antibody for Detection of Helicobacter pylori.

    中村弘毅, 柳井秀雄, 西川潤, 時山裕, 吉田智治, 多田正弘, 沖田極, 松野容子, 星井嘉信

    山口医学   46 ( 4 )   231 - 236   1997.8

     More details

  • 日常遭遇する特異検体の処理と測定上の解決法 ―とくに微生物検査において― Invited

    松野容子, 水野秀一

    Medical Technology   24   139 - 145   1996

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    researchmap

  • 細菌検査の最前線 DNA診断法 1) in situ hybridization (ISH)法 Invited

    松野容子

    Medical Technology   23   233 - 238   1995

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    researchmap

  • 電解質と血液ガス検査 Invited

    松野容子

    臨床看護   21   845 - 854   1995

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    researchmap

  • カテーテル感染 Invited

    松野容子

    検査と技術   21   463 - 464   1993

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    researchmap

▶ display all

Presentations

  • Redundant triplex interactions as a model of lncRNA−chromatin associations in X-chromosome inactivation Invited

    Yoko Matsuno

    International Conference on Liquid Crystals, Liquid Crystalline Polymers and Nanosystems  2019.12 

     More details

    Event date: 2019.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    researchmap

  • Characteristic convergent variation in LINE-1 nucleotides can benefit redundantly forming triplexes with lncRNA in mammalian X-chromosome inactivation International conference

    Yoko Matsuno, Takefumi Yamashita, Michiru Wagatsuma, Hajime Yamakage

    Keystone Symposia―Long Noncoding RNAs: From Molecular Mechanism to Functional Genetics  2019.2  Keystone Symposia on Molecular and Cellular Biology

     More details

    Language:English   Presentation type:Poster presentation  

    Venue:Whistler Conference Center, Whistler, British Columbia, Canada  

    researchmap

  • Characteristic convergent variation in LINE-1 nucleotides can benefit redundantly forming triplexes with lncRNA in mammalian X-chromosome inactivation Invited International conference

    Yoko Matsuno, Takefumi Yamashita, Michiru Wagatsuma, Hajime Yamakage

    Keystone Symposia―Long Noncoding RNAs: From Molecular Mechanism to Functional Genetics conference & RNA‒Protein Interactions conference  2019.2  Keystone Symposia on Molecular and Cellular Biology

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Whistler Conference Center, Whistler, British Columbia, Canada  

    researchmap

  • Characteristic converging variations in nucleotide compositions of mammalian LINEs can benefit forming triplexes with lncRNA in X chromosome inactivation International conference

    Yoko Matsuno, Hajime Yamakage, Peter Borger

    Keystone Symposia on Molecular and Cellular Biology  2018.2 

     More details

    Language:English   Presentation type:Poster presentation  

    Venue:Santa Fe, New Mexico, USA  

    researchmap

  • lncRNA-クロマチン会合モデルからQCMを用いたoligo DNAs-RNA triplexの検証へ Invited

    松野容子

    第11 回QCM研究会 『QCMによる分子間相互作用の測定』  2017.8  ULVAC, Inc. Japan

     More details

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:機械振興会館  

    researchmap

  • A model for lncRNA-chromatin association: Xist/XIST RNA redundantly associates with chromatin through triplex formation Invited

    Yoko Matsuno, Paul Salvaterra, Pieter Borger

    第39回日本分子生物学会年会  2016.11 

     More details

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:パシフィコ横浜  

    有胎盤哺乳類は、雌の胎生初期に性染色体XXの一方をランダムに不活化し、X関連遺伝子の発現を雌雄間で同等にする遺伝子量補正の仕組みを有する。このX染色体不活化機構において、lncRNAであるXist(17kb)/XIST(19kb) RNA は一方のX染色体から発現し、150 Mb以上に及ぶゲノム領域にcisにspreadingしてクロマチンと会合し、染色体単位の不活化を誘導する。しかしながら、Xist/XIST RNAのゲノム上の会合塩基配列は未だ解明されていない。我々はまず、ゲノムとXist/XIST RNAの塩基配列解析から、マウスとヒトに共通して推定される会合モチーフを抽出し、それらの配列特性から、Xist/XIST RNAがH-DNA (intramolecular triplex) 形成の可能なAG-rich simple repeats (SRs)末端を標的とし、dsDNA-RNA (intermolecular triplex)を形成する、lncRNA-クロマチン会合モデルを提唱している。各モチーフはゲノム上に約2000~4000 copy、Xist/XIST RNAにはそれぞれ約200~250 copy存在する。このモデルを検証するため、ex vivo解析としてLNA (locked nucleic acid) 阻害法

    researchmap

  • Localization of Xist/XIST RNAs and their interaction with chromatin Invited International conference

    Yoko Matsuno

    MicroRNAs/Non-Coding RNAs & Genome Edditing Europe-2016 Meeting  2016.11  GeneExpression Systems & Appasani Research Conferences USA & University of Cambridge

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:University of Cambridge, UK  

    Xist/XIST RNA is a long noncoding RNA (lncRNA) that associates with and spreads over the entire X chromosome as a trigger in the process of X chromosome inactivation (XCI), subsequently leading to gene silencing and chromatin conformational change. Using a bioinformatics approach, we identified associations between Xist/XIST RNA and high-frequency AG-rich simple repeats (SRs) that can form H-DNA (intramolecular triplex) structures in both humans and mice. Our data argues for their interaction mediated by termed Junction (J)-motifs preferentially localized to AG-rich SR boundaries and redundant UC-motifs identified in various parts of the Xist/XIST RNA sequences. To examine interactions of these two types of motifs, we performed locked nucleic acid (LNA) interference assay and measurements of circular dichroism (CD) spectrum and quartz crystal microbalance (QCM). LNAs targeting the J-motifs significantly inhibited Xist RNA localization in inducible Xist-transgenic mouse ES cells. Using oligomer dsDNAs and RNAs containing these motifs, CD spectrum and QCM demonstrated the formation of dsDNA-RNA triplexes with a low binding affinity. These results allow for a new model in which Xist/X

    researchmap

  • A model for lncRNA-chromatin association: Xist/XIST RNA redundantly associates with chromatin through triplex formation Invited International conference

    Yoko Matsuno

    MicroRNAs/Noncoding RNAs & Editing Europe & European Neurodegenerative Diseases Europe-2015 meeting  2015.11  GeneExpression Systems & Appasani Research Conferences USA & University of Cambridge

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:University of Cambridge, UK  

    In early female embryos, mouse and human long noncoding Xist/XIST RNA, expressed from the future inactive X chromosome (Xi), triggers X-chromosome inactivation (XCI) by associating with and spreading over the Xi, subsequently leading to gene silencing and structural conversion into heterochromatin. To investigate its association sites on Xi, we initially assumed triplex formation at Xist/XIST RNA repeat E via Hoogsteen hydrogen bonds with dsDNA, and then expanded the possible binding sites to the entire Xist/XIST RNA by defining “redundant UC-motifs”, which are clustered most densely at repeat E and scattered over the remaining regions. We targeted locked nucleic acids (LNAs) at predicted triplex forming sites, termed Junction (J)-motifs, by concatenating two types of AG-rich simple repeats (SRs), resulting in inhibition of Xist/XIST RNA localization in mouse and human cells. DNase I digestion of fixed cells combined with triplex-destabilizer distamycin treatment resulted in enhancement of Xist/XIST RNA signal intensity in RNA FISH when using probes targeting redundant UC-motifs. Here, we propose a model where Xist/XIST RNA and AG-rich SRs bring together reciprocally redundant

    researchmap

  • Xist/XIST RNA and possible triplex formation for localization to chromatin with redundancy International conference

    Matsuno Y, Wutz A, Brown CJ, Salvaterra P

    Keystone Symposia on Molecular and Cellular Biology  2014.2 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Constructing a quick microscopic diagnosis system: a new approach for multi-purposes. International conference

    Matsuno Y, Sadamitsh D, Tamashita S, Tsuruta R, Maekawa T

    The 6th Asian Conference of Clinical Pathology  2000.9 

     More details

    Language:English   Presentation type:Oral presentation (general)  

    Venue:South Korea  

    researchmap

  • Moleculr diagnosis in Laboratories: Prospective roles and problems in Japan Invited International conference

    Matsuno Y, Okayama N

    6th Asian Conference of Clinica Pathology  2000.9 

     More details

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

▶ display all

Research Projects

  • Studies of data-collecting strategies for the literature database on diagnostic characteristics of laboratory examination and structural design of the database

    Grant number:13672436

    2001 - 2003

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    ISHIDA Haku, NISHIBORI Masahiro, MIYAKE Kazunori, KITAMURA Kiyoshi, MATSUNO Youko

      More details

    Grant amount:\3600000 ( Direct Cost: \3600000 )

    The search strategy in identification of the articles related to diagnostic accuracy and a structure of database were investigated for performing systematic review in laboratory medicine efficiently
    When retrieval of article from MEDLINE was performed to identify articles listed in published systematic reviews, the rate of identification of them varied greatly according to the difference in usage of keyword such as MeSH, subheading term or text words. Therefore, in systematic review, in order to perform highly sensitive search without an overlooking, careful thinking for combination of searching terms such as "sensitiv*", "specificit*", "diagnos*" and nomenclature of diagnostic test itself was required based on the result of retrieval investigated by Haynes and others. Moreover, as the structure of a database for systematic review, the database consisting of the following components was considered as suitable : literature information of primary articles, information about summary of systematic review, additional information on the quality and external validity of literature, diagnostic accuracy, and index information such as MeSH
    Based on the database structure, knowledge database with Web interface was developed in order to perform systematic reviews efficiently and continuously through collaborative actions by researchers and the following functions were mounted : 1) displaying articles that have been appraised critically ; 2) a page for registration of literature information of study reports on the database and a page for critical appraisal ; 3) a page for entry of diagnostic characteristics and a function of automatic calculation of confidential intervals ; 4) an input/output page for group-based systematic review ; 5) extraction of information for meta-analyses and graphs (Forest plot and summary ROC curves) : and 6) a page for registration of unpublished studies and a page for editing abstracts. Moreover, the check list for critical appraisal was improved according to the differences between the previous one and other tools such as STARD initiative
    Members of our study group and other collaborative reviewers entered data into this database to evaluate the integrity of the system and reported the system

    researchmap

  • A multiple aimed, dual directional, and visualized data transfer system for bacterial infection control. - A quick microscopic diagnosis system. -

    Grant number:12672242

    2000 - 2001

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    DAIKAI Sadamitsu, HINODA Yuji, TSURUTA Ryosuke, MAEKAWA Tsuyoshi, MATSUNO Youko, YAMASHITA Susumu

      More details

    Grant amount:\3600000 ( Direct Cost: \3600000 )

    A quick microscopic diagnostic system for bacteria (QMDS) is established in Yamaguchi University Hospital. It is extremely useful to save the life of severe septic patient at clinical sites because suitable antibiotics is able to choose at very early stage of his administration.
    This system contains computers with database memory and is connected to our central laboratory, so that central laboratory personnel supports our diagnosis. Patients database can be downloaded from our hospital database. The QMDS is also useful to educate the medical students and residents. The detail of the QMDS is as follows.
    1. A automated aerobic and anaerobic bacterial culture system is set up in The Advanced Medical Emergency and Critical Care Center (AMEC^3). All doctors is trained to perform Gram stain and to use the system at any time for 24 hours.
    2. The main devices are set up in AMEC^3 and connected to the central laboratory of our hospital. Visual data can be transfer is either way, so that diagnostic supports from the central laboratory is instantly possible. The visual data are effectively usable for medical education.
    3. Many kinds of data, such as stained and visualized bacterial data, patient's database from the file of our center and hospital, bacterial data from the central laboratory, educational database and so on, are able to put into the system.
    This new QMDS is useful to understand the bacterial pathophysiology at clinical site in the infectious diseases. The stored visualized data will be effectively used for education. To send huge amount of data between our AMEC^3 and the central laboratory back and forth, and to secure the patient data, a specified LAN between AMEC^3 and our central laboratory must be consisted in near future. Concerning about the educational parts, the visualized quiz type questions with the other clinical data is very unique and effective, which is not exercised by textbooks.
    In conclusion, this QMDS would contain patient's basic data, clinical laboratory data and clinical course data, beside the visualize bacterial data. Therefore, this QMDS has multi-functions and supports not only clinical field, but also educational area.

    researchmap

  • 効率的で良質な医療を目指した病態検査の系統的再評価の基礎的検討

    Grant number:12897028

    2000

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    石田 博, 松野 容子, 北村 聖, 西堀 眞弘, 三宅 一徳

      More details

    Grant amount:\3400000 ( Direct Cost: \3400000 )

    本調査研究では、次の点について実績を得た。
    I.診断検査のEBM確立のための課題の明確化
    診断検査の系統的再評価(systematic review:SR)の手法は未だに開発段階にあり、以下の課題が考えられた。
    A.文献検索手法の模索:文献データベースのindexが不十分なことから、網羅的な検索時には、キーワード検索とフリータームによる検索を行い、論理和で結ぶことが必要である。検査関連用語集や検索例のデータベースを検討すべきである。
    B.一次論文のチェックリストの開発:研究デザインやデータの取り扱いの内的妥当性を検証するもので、投稿論文の採択基準としても活用可能と考えられる。
    C.結果の統合法(メタ分析)の確立:研究結果のばらつきに対してfixed effect、random effectモデルをもとに、サマリーROC(receiver operating characteristics)曲線、尤度比(LR)、オッズ比、識別effect sizeなどの指標で統合されるが、短所・長所があり、使い分けのアルゴリズムなど標準的手法の確立が必要である。
    D.構造化抄録(英文)採用への働きかけ:論文誌における構造化抄録(英文)は一次論文が採択基準に合うかどうかを迅速に見る上で有用である。また、日本語論文の言語バイアスの軽減にもつながると考えられる。
    E.一次研究の登録:診断検査のSRは出版バイアスの影響が大とされるが、根本的な対策はないため、一次研究の登録システムなどを検討すべきである。
    II.データベースとその活用についての戦略
    SRがなされた二次情報を蓄積し公開することで、診断検査のエビデンスを身近なものとし、それらを通して一次研究登録の重要性の認識を高める。また、質の高い多施設間共同研究などを行うために結果登録の可能なデータベースの構築も検討する必要がある。
    III.海外研究グループとの連携
    コクラン共同計画における方法論グループや世界臨床化学会(IFCC)などと連携して効率的にSRを行い、さらにデータベースへの蓄積を行うことで合意し、協力関係を築きつつある。

    researchmap

  • 老化と病的過程における免疫グロブリン 増加とBリンパ球の分化・増殖に関する解析

    Grant number:09772067

    1997 - 1998

    System name:科学研究費助成事業

    Research category:奨励研究(A)

    Awarding organization:日本学術振興会

    松野 容子

      More details

    Grant amount:\2200000 ( Direct Cost: \2200000 )

    I. 免疫グロブリン産生細胞の分離とテロメア長の解析
    (1) 外科的処置により得られたリンパ節より、IgM,IgG,IgAを産生する各形質細胞の分離を行った。昨年度の方法(Fcレセプターを介した非特異的反応を抑制するためのインキュベーション法と通常の抗体を結合させた既製の磁気粒子によるpositive selectionとの組み合わせ)では解析に十分なDNA量を得ることは困難であったが、抗ヒトIg抗体にF(ab')_2を用いて磁気粒子に結合させることで容易にDNAが得られ、テロメア解析に供することが可能となった。
    (2) FI-11-dUTP標識したテロメアプローブ(TTAGGG)_4を用いて、サザンブロットハイリダイゼーションを行い、dioxetane検出系にてテロメア領域を検出し、その平均長をデンシトメーターで計測した。計測方法については、昨年度確立した再現性の良好な方法に従った。
    (3) 本年度に用いた試料でも、昨年度と同様にIgM,IgG,IgA産生細胞の各テロメア長の差は僅かであった。テロメア長短縮の動向は、クラススイッチにより互いに関連すると考えられることから、細胞分裂回数をより詳細に反映できる平均テロメア長以外の解析パラメーターの導入を引き続き試みた。
    II. 免疫グロブリン産生細胞の構成比の解析
    (1) フローサイトメトリーにて免疫グロブリン産生細胞の構成比の解析を行った。解析の再現性は比較的良好であった。この構成比とテロメア長との比較から、Bリンパ球の分化と分裂に関する考察を行った。

    researchmap

 

Teaching Experience

  • 医学研究実習

    2013
    Institution name:新潟大学

  • 早期医学体験実習(EME)

    2012
    -
    2020
    Institution name:新潟大学

  • 統合臨床医学

    2009
    Institution name:新潟大学

  • 臨床実習II(clinical clerkship)

    2009
    -
    2012
    Institution name:新潟大学

  • 医学入門

    2008
    -
    2020
    Institution name:新潟大学

  • 臨床実習IB

    2008
    -
    2020
    Institution name:新潟大学

  • 臨床実習IA

    2008
    -
    2020
    Institution name:新潟大学

▶ display all