記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: This study aimed to investigate the relationship between dental metal allergy, periodontitis, and palmoplantar pustulosis among patients from a dental metal allergy clinic over a period of 8 years. METHODS: This study included 436 patients who visited our dental metal allergy clinic between April 1, 2009 and March 31, 2016. Diagnoses of skin diseases, periodontal records, dental metal series patch test results, and electron probe microanalysis (EPMA) data were obtained from medical records. Relative risk (RR) values were estimated from these data. RESULTS: Of the 359 patients who underwent the patch test, 241 showed a positive reaction. Of the 187 patients who underwent EPMA, 113 had allergenic metals in their dental prostheses. These patients were suspected to have a dental metal allergy. Furthermore, 150 of the 436 patients were diagnosed with palmoplantar pustulosis (PPP). The RR of metal allergy between patients with PPP and healthy subjects was 3.88. The RR of periodontal disease between patients with PPP and PPP-negative patients in the national average was 2.54. CONCLUSIONS: In this study, both dental metal allergy and periodontitis showed a high RR for PPP.

DOI： 10.2186/jpr.JPR_D_20_00307

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY  

OBJECTIVES: Failed implant removal using a high-frequency electrosurgical device (HFED) has been reported to be less invasive than other surgical techniques. We sought to clarify the mechanism of removal torque reduction in an implant by heating with HFED. MATERIALS AND METHODS: Sixty-eight Wistar rats received titanium implants on the maxillary bone 4 weeks after extraction of the first and second molars. The control group was sacrificed 6 weeks after implant installation. In the experimental group, the implant was heated by HFED for 10 s using three different power outputs, and samples were collected at 3, 7, and 14 days after heating. Removal torque measurement and histological analysis were performed in the control and experimental groups. Implant surfaces were observed using an electron-probe microanalyzer (EPMA). Data were analyzed using Mann-Whitney U test at a significance level of 5%. RESULTS: The removal torque could not be measured in the control group due to fracture of the implant. After heating, the removal torque was measurable without fracture and decreased significantly at 14 days as compared with that at 3 days (p < .05). Heating with "min" power output resulted in a significantly smaller blank lacunae area and fewer osteoclasts at 14 days after heating (p < .05). EPMA revealed bone matrix adherence to outer surface of heated implant. CONCLUSIONS: After heating, an enlarged area of blank lacunae around the implant and an increased number of osteoclasts into the bone marrow cavity were observed, which may have contributed to the reduction in removal torque.

DOI： 10.1111/clr.13793

Web of Science

Scopus

PubMed

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

症例の概要:患者は52歳の女性、下顎両側臼歯部欠損による咀嚼障害と下顎前歯部歯列不正と診断された。矯正治療終了後、すれ違い咬合傾向と咬合高径低下が認められた。プロビジョナルブリッジと治療用義歯によって咬合高径を回復し、インプラントプロビジョナルブリッジにより咬合支持を得て、硬質レジン前装冠ブリッジおよび、陶材焼付前装冠ブリッジにより最終補綴を実施した。考察:すれ違い咬合傾向を示す本症例においてはインプラントにより適切な下顎位や咬合支持を回復し、難易度の上昇と咬合崩壊のリスクを回避した。結論:最終補綴により咀嚼機能、審美性の回復、口腔関連QOLの向上が認められた。(著者抄録)

DOI： 10.2186/ajps.12.296

CiNii Article

CiNii Books

researchmap

その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J05334&link_issn=&doc_id=20200818330013&doc_link_id=130007887395&url=http%3A%2F%2Fci.nii.ac.jp%2Fnaid%2F130007887395&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE  

Osteoclast and osteoblast are essential for proper bone development and remodeling as well as recovery of bone fracture. In this study, we seek chemical compounds that enhance turnover of bone metabolism for promoting bone healing. First, we screen a chemical library which includes 378 compounds by using murine pre-osteoclastic RAW264.7 cells to identify compounds that promote osteoclastic differentiation. We find that two ROCK (Rho-associated coiled-coil kinase) inhibitors, HA-1077 (Fasudil) and Y-27632, enhance osteoclastogenesis. Subsequently, we identify that these two compounds also increase osteoblastic differentiation of MC3T3-E1 cells. Finally, our in vivo experiment shows that the local administration of ROCK inhibitors accelerate the bone healing of the rat calvarial defect.

DOI： 10.1016/j.bbrc.2020.03.033

Web of Science

Scopus

PubMed

researchmap

記述言語：日本語   出版者・発行元：大学教育学会  

CiNii Article

CiNii Books

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

The micro- and nanosize surface topography of dental implants has been shown to affect the growth of surrounding cells. In this study, standardized and controlled periodic nanopatterns were fabricated with nanosized surface roughness on titanium substrates, and their influence on bone marrow stromal cells investigated. Cell proliferation assays revealed that the bare substrate with a 1.7 nm surface roughness has lower hydrophilicity but higher proliferation ability than that with a 0.6 nm surface roughness. Further, with the latter substrate, directional cell growth was observed for line and groove patterns with a width of 100 nm and a height of 50 or 100 nm, but not for those with a height of 10 or 25 nm. With the smooth substrate, time-lapse microscopic analyses showed that more than 80% of the bone marrow cells on the line and groove pattern with a height of 100 nm grew and divided along the lines. As the nanosized grain structure controls the cell proliferation rate and the nanosized line and groove structure (50-100 nm) controls cell migration, division, and growth orientation, these standardized nanosized titanium structures can be used to elucidate the mechanisms by which surface topography regulates tissue responses to biomaterials.

DOI： 10.1038/s41598-020-59395-4

Web of Science

Scopus

PubMed

researchmap

researchmap

DOI： 10.1111/jdv.15434

Scopus

PubMed

researchmap

記述言語：日本語   出版者・発行元：公益社団法人 日本補綴歯科学会  

<p> この度，4年ぶりに歯科補綴学専門用語集第4版が改訂され，2019年3月25日に第5版が発刊された．第5版では学問と時代の変化に伴って新たな用語が多く収載されたこと，また旧版収載用語のうち，用語やその意味が変わったものが多々ある．</p><p> 本稿では，用語集第5版の改訂の流れ，新規収載用語ならびに変更内容を解説するとともに，今後の用語検討の在り方に関する委員会案についても紹介する．</p><p> 本稿を通じて読者の皆様が新たな歯科補綴学専門用語集を存分に活用していただければ幸いである．</p>

DOI： 10.2186/ajps.11.309

CiNii Article

CiNii Books

researchmap

その他リンク： http://search.jamas.or.jp/link/ui/2020054514

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE  

Bone augmentation therapy is used in dental implantation. While techniques to induce bone formation are generally successful, the maintenance of bone mass is more difficult. Therefore, it is important to understand the mechanisms that regulate this process. Insulin-like growth factor-1 (IGF-1) is one of the most abundant growth factors that regulate bone mass, promote osteoblast differentiation, and accelerate bone formation. The activity of IGF-1 is regulated by IGF-binding proteins (IGFBPs). IGFBP-3 forms a ternary complex with IGF-1, extending its half-life in the circulating system. Therefore, IGFBP-3 acts as a stabilizer and transporter of IGF-1. Recent studies reported new IGF-1-independent functions of IGFBP-3 related with bone metabolism. In this study, we investigated the function of IGFBP-3 in osteoblast differentiation. Our results showed that IGFBP-3 decreases the expression of osteoblast differentiation markers, whose expression is enhanced by bone morphogenetic protein-2 (BMP-2). IGFBP-3 also reduced BMP-2 effect on ALP activity and mineral nodule formation. In addition, IGFBP-3 suppresses the activity of the Smad Binding Element (SBE) reporter, induced by BMP-2 signaling. These results suggest that IGFBP-3 inhibits osteoblast differentiation through the BMP-2 signal pathway, and that IGFBP-3 might play a role in bone mass maintenance in an IGF-1-dependent and -independent manner.

DOI： 10.1016/j.bbrc.2018.11.065

Web of Science

Scopus

PubMed

researchmap

掲載種別：研究論文（学術雑誌）  

DOI： 10.4236/ojst.2018.82005

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PUBLIC LIBRARY SCIENCE  

Fibrillar type I collagen, the predominant organic component in bone, is stabilized by lysyl oxidase (LOX)-initiated covalent intermolecular cross-linking, an important determinant of bone quality. However, the impact of collagen cross-linking on the activity of bone cells and subsequent tissue remodeling is not well understood. In this study, we investigated the effect of collagen cross-linking on bone cellular activities employing a loss-of-function approach, using a potent LOX inhibitor, β-aminopropionitrile (BAPN). Osteoblastic cells (MC3T3-E1) were cultured for 2 weeks in the presence of 0-2 mM BAPN to obtain low cross-linked collagen matrices. The addition of BAPN to the cultures diminished collagen cross-links in a dose-dependent manner and, at 1 mM level, none of the major cross-links were detected without affecting collagen production. After the removal of cellular components from these cultures, MC3T3-E1, osteoclasts (RAW264.7), or mouse primary bone marrow-derived stromal cells (BMSCs) were seeded. MC3T3-E1 cells grown on low cross-link matrices showed increased alkaline phosphatase (ALP) activity. The number of multinucleate tartrate-resistant acid phosphatase (TRAP)-positive cells increased in RAW264.7 cells. Initial adhesion, proliferation, and ALP activity of BMSCs also increased. In the animal experiments, 4-week-old C57BL/6 mice were fed with BAPN-containing diet for 8 weeks. At this point, biochemical analysis of bone demonstrated that collagen cross-links decreased without affecting collagen content. Then, the diet was changed to a control diet to minimize the direct effect of BAPN. At 2 and 4 weeks after the change, histological samples were prepared. Histological examination of femur samples at 4 weeks showed a significant increase in the number of bone surface osteoblasts, while the bone volume and surface osteoclast numbers were not significantly affected. These results clearly demonstrated that the extent of collagen cross-linking of bone matrix affected the differentiation of bone cells, underscoring the importance of collagen cross-linking in the regulation of cell behaviors and tissue remodeling in bone. Characterization of collagen cross-linking in bone may be beneficial to obtain insight into not only bone mechanical property, but also bone cellular activities.

DOI： 10.1371/journal.pone.0204306

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   出版者・発行元：WILEY  

Web of Science

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Background/Objectives: The periodontal ligament (PDL) is a non-mineralized connective tissue that exists between the alveolar bone and root surface cementum and plays important roles in tooth function. The PDL harbors a remarkable reserve of multipotent stem cells, which maintain various types of cells. However, the sources of these stem cells, other than their developmental origin, are not well understood. Material and Methods: To elucidate the recruitment of bone marrow (BM)-derived stem cells in the PDL, green fluorescent protein (GFP)-expressing BM-derived cells were transplanted into the femoral BM of immunodeficient rats, and the distribution and expression of stem cell markers in the PDL were analyzed in vivo. To evaluate the functional significance of BM-derived cells to the PDL, tooth replantation was performed and the expression of stromal cell-derived factor (SDF)-1, a critical chemotactic signal for mesenchymal stem cell recruitment, was analyzed. To confirm the SDF-1-dependency of BM-derived cell migration to the PDL, PDL-conditioned medium (CM) was prepared, and BM-derived cell migration was analyzed using a transwell culture system. Results: Four weeks after cell transplantation, GFP-positive cells were detected in the PDL, and some of them were also positive for stem cell markers (i.e., CD29, SSEA4, and αSMA). Seven days after tooth replantation, the number of GFP- and SDF-1-positive cells significantly increased in PDL. Concurrently, the concentration of SDF-1 and the number of colony-forming units of fibroblasts in peripheral blood were increased. BM-derived cell migration increased in PDL-CM and was inhibited by an inhibitor of C-X-C chemokine receptor type 4 (CXCR4), an SDF-1 receptor. Conclusion: These results indicate that stem cells and their progeny in PDL are not only derived from their developmental origin but are also supplied from the BM via the blood as the need arises. Moreover, this BM-derived cell recruitment appears to be regulated, at least partially, by the SDF-1/CXCR4 axis.

DOI： 10.1111/jre.12433

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY  

PURPOSE: Zirconia is a good candidate material in the dental field. In this study, we evaluated biological responses against a zirconia drill using a bone cavity healing model. MATERIALS AND METHODS: Zirconia drills, stainless steel drills, and the drilled bone surface were observed by scanning electron microscopy (SEM), before and after cavity preparation. For the bone cavity healing model, the upper first and second molars of Wistar rats were extracted. After 4 weeks, cavities were prepared with zirconia drills on the left side. As a control, a stainless steel drill was used on the right side. At 3, 7, and 14 days after surgery, micro-CT images were taken. Samples were prepared for histological staining. RESULTS: SEM images revealed that zirconia drills maintained sharpness even after 30 drilling procedures. The bone surface was smoother with the zirconia drill. Micro-CT images showed faster and earlier bone healing in the zirconia drill cavity. On H-E staining, at 7 days, the zirconia drill defect had a smaller blank lacunae area. At 14 days, the zirconia drill defect was filled with newly formed bone. CONCLUSIONS: The zirconia drill induces less damage during cavity preparation and is advantageous for bone healing. (197 words).

DOI： 10.1111/cid.12452

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY  

Type I collagen, a major extracellular component of the periodontal ligament (PDL), is post-translationally modified by a series of specific enzymes. Among the collagen-modifying enzymes, lysyl oxidase (LOX) is essential to initiate collagen cross-linking and lysyl hydroxylases (LHs) to regulate the cross-linking pathways that are important for tissue specific mechanical properties. The purpose of this study was to investigate the effects of mechanical loading on the expression of collagen-modifying enzymes and subsequent tissue changes in PDL. Primary human PDL cells were subjected to mechanical loading in a 3D collagen gel, and gene expression and collagen component were analyzed. Wistar rats were subjected to excessive occlusal loading with or without intra-peritoneal injection of a LOX inhibitor, β-aminopropionitrile (BAPN). Upon mechanical loading, gene expression of LH2 and LOX was significantly elevated, while that of COL1A2 was not affected on hPDL-derived cells. The mechanical loading also elevated formation of collagen α-chain dimers in 3D culture. The numbers of LH2 and LOX positive cells in PDL were significantly increased in an excessive occlusal loading model. Notably, an increase of LH2-positive cells was observed only at the bone-side of PDL. Intensity of picrosirius red staining was increased by excessive occlusal loading, but significantly diminished by BAPN treatment. These results demonstrated that mechanical loading induced collagen maturation in PDL by up-regulating collagen-modifying enzymes and subsequent collagen cross-linking which are important for PDL tissue maintenance. J. Cell. Physiol. 231: 926-933, 2016. © 2015 Wiley Periodicals, Inc.

DOI： 10.1002/jcp.25184

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bbrep.2015.12.011

Scopus

PubMed

researchmap

記述言語：英語   出版者・発行元：(公社)日本薬理学会  

Web of Science

researchmap

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

researchmap

記述言語：英語   出版者・発行元：(公社)日本補綴歯科学会  

Alveolar ridge plays a pivotal role in supporting dental prosthesis particularly in edentulous and semi-dentulous patients. However the alveolar ridge undergoes atrophic change after tooth loss. The vertical and horizontal volume of the alveolar ridge restricts the design of dental prosthesis; thus, maintaining sufficient alveolar ridge volume is vital for successful oral rehabilitation. Recent progress in regenerative approaches has conferred marked benefits in prosthetic dentistry, enabling regeneration of the atrophic alveolar ridge. In order to achieve successful alveolar ridge augmentation, sufficient numbers of osteogenic cells are necessary; therefore, autologous osteoprogenitor cells are isolated, expanded in vitro, and transplanted to the specific anatomical site where the bone is required. Recent studies have gradually elucidated that transplanted osteoprogenitor cells are not only a source of bone forming osteoblasts, they appear to play multiple roles, such as recruitment of endogenous osteoprogenitor cells and immunomodulatory function, at the forefront of bone regeneration. This review focuses on the current consensus of cell-based bone augmentation therapies with emphasis on cell sources, transplanted cell survival, endogenous stem cell recruitment and immunomodulatory function of transplanted osteoprogenitor cells. Furthermore, if we were able to control the mobilization of endogenous osteoprogenitor cells, large-scale surgery may no longer be necessary. Such treatment strategy may open a new era of safer and more effective alveolar ridge augmentation treatment options.

DOI： 10.1016/j.jpor.2015.02.001

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：W B SAUNDERS CO-ELSEVIER INC  

PURPOSE: Clinically, bone marrow stromal cells (BMCs) are the most common source of osteoprogenitor cells. Its harvest process, however, is invasive to patients. Previous reports have shown the potential advantages of using periosteum-derived cells (PDCs) as a source of cell-based transplant therapy. The objective of our study was to characterize the osteoblastic differentiation and mineralization ability of PDCs versus BMCs and osteoblasts (OBs). MATERIALS AND METHODS: BMCs, OBs, and PDCs were isolated from 4-week-old male Wistar rats. To characterize the differentiation ability of the cells, MTS assay, alkaline phosphatase (ALP) activity staining, picrosirius red staining, and alizarin red staining were performed. Immunohistochemistry was performed on paraffin sections of calvarial periosteum to determine the presence of mesenchymal stem cells. RESULTS: PDCs showed the greatest proliferation rate compared with BMCs and OBs. Matured collagenous matrix formation was observed in PDCs and BMCs. ALP-positive cells and in vitro mineralization were evident in all cell types analyzed; however, that of PDCs was not comparable to that of the OBs and BMCs. Immunohistochemistry revealed the presence of STRO-1-and CD105-positive cells in the cambium layer of the periosteum. CONCLUSIONS: PDCs have remarkable proliferative ability, but contain only a small population of osteogenic cells compared with BMCs and OBs. Although cell activity can be affected by various factors, such as age, culture condition, additives, and so forth, PDCs are likely not the source of OBs, although they might provide matrices that indirectly aid in bone formation.

DOI： 10.1016/j.joms.2013.12.001

Web of Science

Scopus

PubMed

researchmap

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本歯科医学会  

ヒストン脱アセチル化酵素阻害剤(HDACI)は抗癌剤、抗てんかん薬として臨床応用される薬剤である。HDACIは遺伝子発現過程においてクロマチンリモデリングに関与し、遺伝子発現を活性化することが知られている。骨形成関連においてはRunx2の安定化に関与し骨芽細胞の分化を促進する作用が報告されている。そこで本研究では、はじめに上顎骨円筒形骨欠損修復モデルを用いてHDACIの全身投与における骨形成能の賦活化を検討することとした。HDACIにはバルプロ酸(Valproic acid(VPA))を使用し、投与法は腹腔内投与、投与期間は円筒形骨欠損形成前7日間とした。VPA全身投与群では窩洞内新生骨形成能促進、円筒形骨欠損治癒促進を示す像が窩洞形成14日から21日後にかけて観察された。次に頭蓋骨限界径骨欠損細胞移植モデルを作製し、大腿骨より採取した骨髄間質細胞をVPA処理し欠損部へ移植した。VPA処理細胞移植窩洞では対照群と比較して窩洞内の石灰化の亢進が観察された。本研究より骨増成法におけるHDACIの有効性が示され、更にHDACIの多機能性を応用した新規骨増成法開発の可能性が示唆された。(著者抄録)

J-GLOBAL

researchmap

その他リンク： https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792214/

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.joms.2013.12.001

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Defining the molecular mechanisms that underlie development and maintenance of neuronal phenotypic diversity in the CNS is a fundamental challenge in developmental neurobiology. The vast majority of olfactory bulb (OB) interneurons are GABAergic and this neurotransmitter phenotype is specified in migrating neuroblasts by transcription of either or both glutamic acid decarboxylase 1 (Gad1) and Gad2. A subset of OB interneurons also co-express dopamine, but transcriptional repression of tyrosine hydroxylase (Th) suppresses the dopaminergic phenotype until these neurons terminally differentiate. In mature OB interneurons, GABA and dopamine levels are modulated by odorant-induced synaptic activity-dependent regulation of Gad1 and Th transcription. The molecular mechanisms that specify and maintain the GABAergic and dopaminergic phenotypes in the OB are not clearly delineated. In this report, we review previous studies and present novel findings that provide insight into the contribution of epigenetic regulatory mechanisms for controlling expression of these neurotransmitter phenotypes in the OB. We show that HDAC enzymes suppress the dopaminergic phenotype in migrating neuroblasts by repressing Th transcription. In the mature interneurons, both Th and Gad1 transcription levels are modulated by synaptic activity-dependent recruitment of acetylated Histone H3 on both the Th and Gad1 proximal promoters. We also show that HDAC2 has the opposite transcriptional response to odorant-induced synaptic activity when compared to Th and Gad1. These findings suggest that HDAC2 mediates, in part, the activity-dependent chromatin remodeling of the Th and Gad1 proximal promoters in mature OB interneurons.

DOI： 10.1016/j.ijdevneu.2012.11.009

Web of Science

Scopus

PubMed

researchmap

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SOC NEUROSCIENCE  

A recent study proposed that differentiation of dopaminergic neurons requires a conserved "dopamine motif" (DA-motif) that functions as a binding site for ETS DNA binding domain transcription factors. In the mammalian olfactory bulb (OB), the expression of a set of five genes [including tyrosine hydroxylase (Th)] that are necessary for differentiation of dopaminergic neurons was suggested to be regulated by the ETS-domain transcription factor ER81 via the DA-motif. To investigate this putative regulatory role of ER81, expression levels of these five genes were compared in both olfactory bulbs of adult wild-type mice subjected to unilateral naris closure and the olfactory bulbs of neonatal Er81 wild-type and mutant mice. These studies found that ER81 was necessary only for Th expression and not the other cassette genes. Chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays (EMSA) experiments showed that ER81 bound directly to a consensus binding site/DA-motif in the rodent Th proximal promoter. However, the ER81 binding site/DA-motif in the Th proximal promoter is poorly conserved in other mammals. Both ChIP assays with canine OB tissue and EMSA experiments with the human Th proximal promoter did not detect ER81 binding to the Th DA-motif from these species. These results suggest that regulation of Th expression by the direct binding of ER81 to the Th promoter is a species-specific mechanism. These findings indicate that ER81 is not necessary for expression of the OB dopaminergic gene cassette and that the DA-motif is not involved in differentiation of the mammalian OB dopaminergic phenotype.

DOI： 10.1523/JNEUROSCI.0419-10.2010

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE  

Most olfactory bulb (OB) interneurons are derived from neural stem cells in the subventricular zone (SVZ) and migrate to the OB via the rostral migratory stream (RMS). Mature dopaminergic interneurons in the OB glomerular layer are readily identified by their synaptic activity-dependent expression of tyrosine hydroxylase (TH). Paradoxically, TH is not expressed in neural progenitors migrating in the RMS, even though ambient GABA and glutamate depolarize these progenitors. In forebrain slice cultures prepared from transgenic mice containing a GFP reporter gene under the control of the Th 9kb upstream regulatory region, treatment with histone deacetylase (HDAC) inhibitors (either sodium butyrate, Trichostatin A or Scriptaid) induced Th-GFP expression specifically in the RMS independently of depolarizing conditions in the culture media. Th-GFP expression in the glomerular layer was also increased in slices treated with Trichostatin A, but this increased expression was dependent on depolarizing concentrations of KCl in the culture media. Th-GFP expression was also induced in the RMS in vivo by intra-peritoneal injections with either sodium butyrate or valproic acid. Quantitative RT-PCR analysis of neurosphere cultures confirmed that HDAC inhibitors de-repressed Th expression in SVZ-derived neural progenitors. Together, these findings suggest that HDAC function is critical for regulating Th expression levels in both neural progenitors and mature OB dopaminergic neurons. However, the differential responses to the combinatorial exposure of HDAC inhibitors and depolarizing culture conditions indicate that Th expression in mature OB neurons and neural progenitors in the RMS are regulated by distinct HDAC-mediated mechanisms.

DOI： 10.1016/j.bbrc.2010.02.054

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   出版者・発行元：OXFORD UNIV PRESS  

Web of Science

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY  

gamma-Aminobutyric acid (GABA) regulates the proliferation and migration of olfactory bulb (OB) interneuron progenitors derived from the subventricular zone (SVZ), but the role of GABA in the differentiation of these progenitors has been largely unexplored. This study examines the role of GABA in the differentiation of OB dopaminergic interneurons using neonatal forebrain organotypic slice cultures prepared from transgenic mice expressing green fluorescent protein (GFP) under the control of the tyrosine hydroxylase (Th) gene promoter (ThGFP). KCl-mediated depolarization of the slices induced ThGFP expression. The addition of GABA to the depolarized slices further increased GFP fluorescence by inducing ThGFP expression in an additional set of periglomerular cells. These findings show that GABA promoted differentiation of SVZ-derived OB dopaminergic interneurons and suggest that GABA indirectly regulated Th expression and OB dopaminergic neuron differentiation through an acceleration of the maturation rate for the dopaminergic progenitors. Additional studies revealed that the effect of GABA on ThGFP expression required activation of L- and P/Q-type Ca2+ channels as well as GABA(A) and GABA(B) receptors. These voltage-gated Ca2+ channels and GABA receptors have previously been shown to be required for the coexpressed GABAergic phenotype in the OB interneurons. Together, these findings suggest that Th expression and the differentiation of OB dopaminergic interneurons are coupled to the coexpressed GABAergic phenotype and demonstrate a novel role for GABA in neurogenesis.

DOI： 10.1002/jnr.22055

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

In the adrenal medulla, binding of the immediate early gene (IEG) proteins, EGR-1 (ZIF-268/KROX-24/NGFI-A) and AP-1, to the tyrosine hydroxylase (Th) proximal promoter mediate inducible Th expression. The current study investigated the potential role of EGR-1 in inducible Th expression in the olfactory bulb (OB) since IEGs bound to the AP-1 site in the Th proximal promoter are also necessary for activity-dependent OB TH expression. Immunohistochemical analysis of a naris-occluded mouse model of odor deprivation revealed weak EGR-1 expression levels in the OB glomerular layer that were activity-dependent. Immunofluorescence analysis indicated that a majority of glomerular cells expressing EGR-1 also co-expressed TH, but only small subset of TH-expressing cells contained EGR-1. By contrast, granule cells, which lack TH, exhibited EGR-1 expression levels that were unchanged by naris closure. Together, these finding suggest that EGR-1 mediates activity-dependent TH expression in a subset of OB dopaminergic neurons, and that there is differential regulation of EGR-1 in periglomerular and granule cells.

DOI： 10.1007/s10735-009-9217-2

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   出版者・発行元：OXFORD UNIV PRESS  

Web of Science

researchmap

記述言語：英語   出版者・発行元：OXFORD UNIV PRESS  

Web of Science

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：国際組織細胞学会  

Root resorption lacunae are principally formed by odontoclasts. While these cells develop from the same origin as osteoclasts, odontoclasts normally have fewer nuclei and a less clear zone compared with osteoclasts. We therefore, hypothesized that odontoclasts possess less differentiation in matrix resorption characteristics than osteoclasts. To test our hypothesis, we compared the TRAP-positive area and the expression patterns of two important proteolytic enzymes, cathepsin K and matrix metalloproteinase-9 (MMP-9), between odontoclasts and osteoclasts. We focused on physiological root resorption in the rat molar, which is a useful experimental model for estimating odontoclasts and osteoclasts. Observations showed the number of nuclei and the TRAP-positive area of odontoclasts to be significantly less compared with osteoclasts. Using in situ hybridization and double labeling fluorescence in situ hybridization showed the majority of odontoclasts to express both cathepsin K and MMP-9, especially 4 and 5 weeks of age, when physiological root resorption occurs actively. Moreover, putative precursor cells of odontoclasts, which typically appeared in the middle of the periodontal ligament at 3 weeks of age, expressed both enzymes. In contrast, the majority of matured osteoclasts expressed only cathepsin K but not MMP-9. We suggest that odontoclasts are comparable to osteoclasts with less differentiation with regard to the expression of proteolytic enzymes.

DOI： 10.1679/aohc.71.89

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-LISS  

The mechanisms underlying dopamine (DA) phenotypic differentiation in the olfactory bulb (OB) have not yet been fully elucidated and are the subject of some controversy. OB DA interneurons destined for the glomerular layer were shown to originate in the subventricular zone (SVZ) and in the rostral migratory stream (RMS). The current study investigated whether calcium/calmodulin-dependent protein kinase IV (CaMKIV) either alone or together with the Ets transcription factor ER81 was necessary for phenotypic determination during migration of progenitors. In most brain areas, including the OB, CaMKIV and ER81 displayed a reciprocal distribution. In the SVZ, only ER81 could be demonstrated. In the RMS, a subpopulation of progenitors contained ER81, but few, if any, contained CaMKIV. In OB, CaMKIV expression, restricted to deep granule cells, showed limited overlap with ER81. ER81 expression was weak in deep granule cells. Strong labeling occurred in the mitral and glomerular layers, where ER81 colabeled dopaminergic periglomerular cells that expressed either tyrosine hydroxylase (TH) or green fluorescent protein, the latter reporter gene under control of 9-kb of 5' TH promoter. Odor deprivation resulted in a significant 5.2-fold decline in TH immunoreactivity, but ER81 exhibited a relatively small 1.7-fold decline in immunoreactivity. TH expression as well as brain and bulb size were unchanged in CaMKIV knockout mice. These data suggest that ER81 may be required but is not sufficient for DA neuron differentiation and that CaMKIV is not directly involved in TH gene regulation.

DOI： 10.1002/cne.21293

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER/PLENUM PUBLISHERS  

The mammalian olfactory bulb (OB) is among the few regions in adult brain which generates interneurons. A subpopulation of these phenotypically diverse interneurons is dopaminergic (DA) periglomerular cells. Full phenotypic development as indicated by expression of tyrosine hydroxylase (TH), the first enzyme in DA biosynthesis, requires afferent activity or equivalent depolarizing conditions. To investigate the hypothesis that cFOS regulates TH expression, this study analyzed OB slice cultures obtained from neonatal transgenic mice expressing 9 kb of TH promoter directing expression of green fluorescent protein (TH/GFP). Cultures were depolarized with 50 mM potassium chloride (KCl), the calcium channel blocker, nifedipine (10 mu M) with KCl, or an equimolar concentration of sodium chloride (NaCl). Depolarization increased cFOS expression 6-fold peaking at about 3 h. Staining decreased rapidly returning to control, NaCl, levels by 48 h post-stimulation when TH/GFP expression was highest. Nifedipine blocked the increase in TH and cFOS suggesting that similar signal transduction pathways mediate both responses.

DOI： 10.1007/s11064-006-9134-7

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

The gene expression for seven phosphatidylinositol phosphate kinases (PIPKs)-types Ialpha, Ibeta, Igamma, types IIalpha, IIbeta, IIgamma, and type III-was examined using in situ hybridization histochemistry, in the mouse brain during normal development. In the embryonic mouse brain, positive expression signals were detected only for the genes encoding PIPK Igamma and PIPK IIbeta in both the cerebral ventricular and mantle zones, with weaker signals in the former zone. On the other hand, the genes encoding all PIPKs were essentially detected in the external granule cell layer which represents the germinal zone for the neuronal granule cells. In the postnatal brain, among the seven PIPKs, the expression for genes encoding PIPK Igamma and IIbeta is evident in most gray matter, while the expression for the other five types was weak in the cortical gray matter and negligible in most non-cortical gray matter such as the diencephalon and brain stem nuclei. While the expression for most PIPKs in the mature hippocampus was distinct, the expression in the CA3 and the dentate gyrus was less definite for the genes encoding PIPK Ialpha and IIgamma, respectively. The distinct expression for the gene encoding PIPK IIalpha was detected in the postnatal white matter such as the cerebellar medulla, the corpus callosum, the hippocampal fimbriae, and the internal capsule.

DOI： 10.1016/S1567-133X(01)00023-0

Scopus

PubMed

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

記述言語：日本語   出版者・発行元：日本バイオマテリアル学会  

デンタルインプラントは優れた治療戦略であり臨床成績も良好だ,オッセオインテグレーションの成立機序の詳細は解明されていない.粗面構造が骨形成を促進することは知られており,構造形成はマイクロレベルからナノレベルにまで及んでいる.しかし現在までに,チタンのどのような構造因子やどのようなチタンへの接着蛋白質が骨形成の促進に関与するかに関する解析は進んでいない.我々は,チタン上に形成される粗面構造が,技術的制約から非規格化構造であることに,原因の一端があると考え,規格化ナノ構造によるオッセオインテグレーション機構解明とその制御を目指した研究を進めている.(著者抄録)

CiNii Books

researchmap

その他リンク： https://ndlsearch.ndl.go.jp/books/R000000004-I032839032

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

CiNii Books

researchmap

その他リンク： https://ndlsearch.ndl.go.jp/books/R000000004-I032288669

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

近年,非金属材料補綴装置の保険収載が進み,歯科金属アレルギーが原因として疑われる皮膚粘膜疾患患者に対し,負担の少ない金属補綴装置の除去置換療法が可能となった.金属除去置換療法による皮膚粘膜症状軽快の報告は多いが,金属除去置換処置で皮膚粘膜症状の軽快,消退が認められなかった患者も存在する.原因はさまざまに想定し得るが,検査,診断,立案した治療方針に問題がある可能性も考えられる.本報告は2016年の日本補綴歯科学会雑誌における総説「歯科金属アレルギーの現状と展望」を補完,または再確認する形で,検査,診断,治療方針や他科連携について提案する.(著者抄録)

researchmap

その他リンク： https://search.jamas.or.jp/default/link?pub_year=2022&ichushi_jid=J05334&link_issn=&doc_id=20220726230004&doc_link_id=10.2186%2Fajps.14.250&url=https%3A%2F%2Fdoi.org%2F10.2186%2Fajps.14.250&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_2.gif

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

記述言語：日本語   出版者・発行元：(一社)日本総合健診医学会  

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

J-GLOBAL

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：日本バイオマテリアル学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)日本歯科医学教育学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(株)第一歯科出版  

CiNii Books

J-GLOBAL

researchmap

その他リンク： https://ndlsearch.ndl.go.jp/books/R000000004-I032530536

記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟歯学会  

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟歯学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟歯学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟歯学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：全日本病院出版会  

歯科において治療対象となる扁平苔癬は口腔扁平苔癬である。口腔扁平苔癬は原因不明の難治性疾患で、潜在性悪性疾患と認識されている。頬、歯肉、舌、口唇などの口腔粘膜に発症し、白色の角化性病変を基本とするが、進行により紅斑を呈し、びらんを生ずることもある。重篤化すると疼痛により摂食困難となる。歯科的対応としては症状の軽減を目的とした口腔衛生指導、含嗽、ステロイド局所塗布などが実施されている。金属アレルギーの関与、金属除去の効果について、現段階では水銀以外は口腔扁平苔癬発症・症状軽快との関連に明確なエビデンスは存在していないとされている。しかし口腔衛生指導、ステロイド局所塗布が奏効しなかった症例において、金属除去によって粘膜症状が軽快した症例も報告されている。(著者抄録)

CiNii Article

CiNii Books

J-GLOBAL

researchmap

その他リンク： http://search.jamas.or.jp/link/ui/2019185227

記述言語：日本語   出版者・発行元：日本歯科医学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)日本歯科医学教育学会  

J-GLOBAL

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：日本歯科医学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(株)北隆館  

歯科に用いられている各種合金が金属アレルギーの原因となり得ることが知られている。ところが、臨床的にはその存在がほぼ確実であるにもかかわらず、基礎研究や臨床研究の遅れにより、明確な診断・治療方法は見出されていない。歯科金属アレルギーはそのほとんどが全身性接触皮膚炎として病態を現す。今後は病態と金属の種類ごとにその発症メカニズムを解明し、診断・治療に資する成果が求められている。本稿ではその歴史、実態、代表的症状と現状での問題点を概説し、歯科金属アレルギーに関する理解を深める一助としたい。(著者抄録)

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)日本歯科医学教育学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)日本歯科理工学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟歯学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)日本骨代謝学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本口腔インプラント学会  

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：公益社団法人 日本補綴歯科学会  

<p> 歯科金属アレルギーは口腔内の金属補綴修復物に含まれる金属元素をアレルゲンとしてアレルギー反応が感作，惹起され，局所性，全身性の接触皮膚炎を病態とする疾患として理解されている．歯科金属アレルギーと関連疾患に対する，検査，診査，診断，治療法などに関する診療ガイドラインは，現在のところ策定されていない．本総説は歯科金属アレルギー診療ガイドライン策定に必要な臨床研究，基礎研究や，歯科金属アレルギーに関する臨床について，現状と展望を解説するものである．</p>

DOI： 10.2186/ajps.8.327

CiNii Article

CiNii Books

researchmap

その他リンク： http://search.jamas.or.jp/link/ui/2017051148

記述言語：日本語   出版者・発行元：(一社)日本骨代謝学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)歯科基礎医学会  

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2015&ichushi_jid=J05334&link_issn=&doc_id=20150731230004&doc_link_id=%2Fdv5proso%2F2015%2F000703%2F006%2F0234-0235%26dl%3D0&url=http%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdv5proso%2F2015%2F000703%2F006%2F0234-0235%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟歯学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)日本歯科医学教育学会  

J-GLOBAL

researchmap

記述言語：日本語  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：日本歯科医学会  

本研究では、歯科用金属アレルギーの臨床病態と補綴学的対応の効果を明らかにすることを目的に、1)多施設における歯科用金属アレルギーのアンケートによる実態調査、2)歯科用金属アレルギーの疫学研究に関する文献的考察を行った。その結果、歯科用金属アレルギーの臨床病態と歯科的対応の問題点が明らかになった。とくに近年アレルギー陽性率が上昇しているニッケルやパラジウムは歯科用合金にも使用されており、対応が急務であることが示唆された。(著者抄録)

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟歯学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟歯学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)歯科基礎医学会  

researchmap

記述言語：日本語   出版者・発行元：(一社)歯科基礎医学会  

researchmap

記述言語：日本語   出版者・発行元：(一社)歯科基礎医学会  

researchmap

記述言語：英語   出版者・発行元：新潟歯学会  

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟歯学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：新潟大学教育・学生支援機構  

良質な歯科医療には適切な診断、治療計画、治療技術が不可欠である。新潟大学歯学部では臨床教育において早期臨床実習、統合型PBL、総合模型実習、参加実践型臨床実習を行っているが、ーロ腔単位での治療計画立案の機会は十分とはいえない。本演習は、治療計画の立案能力養成を目的に、実際の症例からデータを提供、学生は治療計画立案し、アドバイザーは治療計画の妥当性を評価、助言し、学生は助言を基に再度立案を行う。演習効果について演習終了後にアンケート調査を行う。本演習を通して治療計画立案の基礎を習得、治療方針の多様性に触れ、卒後歯科医師臨床研修における包括的診療能力の獲得と、各実習の有機的連携を期待する。

CiNii Article

CiNii Books

researchmap

その他リンク： http://hdl.handle.net/10191/24051

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)歯科基礎医学会  

researchmap

記述言語：日本語   出版者・発行元：(一社)歯科基礎医学会  

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)日本歯科医学教育学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本歯科医師会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本歯科医師会  

J-GLOBAL

researchmap

記述言語：英語   出版者・発行元：新潟歯学会  

researchmap

記述言語：英語   出版者・発行元：新潟歯学会  

researchmap

記述言語：英語   出版者・発行元：(公社)日本口腔インプラント学会  

researchmap

記述言語：英語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

記述言語：日本語   出版者・発行元：日本歯科医学会  

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(一社)歯科基礎医学会  

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：東北大学歯学会  

I型コラーゲンは骨や結合組織,象牙質に豊富に含まれる線維状タンパクである。I型コラーゲンC-プロペプチドはコラーゲン分子への変換に伴って産生され,コラーゲン分子の会合とコラーゲン線維形成にとって必須である。近年,C-プロペプチド単体に様々な作用があることが報告され,特にin vitroにおいて硬組織形成に関与する前駆細胞の分化に抑制的に働くことが報告されている。つまり,硬組織の発達・形成過程での重要性が示されているが,in vivoにおけるC-プロペプチドの局在については明らかとなっていない。本研究では,抗I型コラーゲンC-プロペプチド抗体を用いた免疫組織染色を行い,骨および象牙質,セメント質といった硬組織領域における,それぞれの発現局在について調べ,抗I型コラーゲン抗体を用いたIHCとプロコラーゲンα2(I)鎖のcRNAプローブを用いたin situ hybridizationの結果と比較検討した。胎生期における下顎骨発生,および歯牙の発生過程におけるC-プロペプチドの局在から,骨,象牙質およびセメント質の活発な形成時期にはその基質において集積が認められた。くわえて,ヘルトウィッヒ上皮鞘周囲に認められたI型コラーゲンC-プロペプチドの集積の所見からも,すでに報告されている分化調節機構が骨組織のみならず,象牙質やセメント質の発達過程においても重要である可能性が示された。

CiNii Article

CiNii Books

J-GLOBAL

researchmap

その他リンク： http://search.jamas.or.jp/link/ui/2008179107

記述言語：日本語   出版者・発行元：東北大学歯学会  

CiNii Article

CiNii Books

researchmap

その他リンク： http://search.jamas.or.jp/link/ui/2005091088

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

researchmap

記述言語：日本語   出版者・発行元：歯科基礎医学会  

CiNii Article

CiNii Books

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

CiNii Article

CiNii Books

researchmap

記述言語：日本語   出版者・発行元：歯科基礎医学会  

CiNii Article

CiNii Books

J-GLOBAL

researchmap

その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2003&ichushi_jid=J00559&link_issn=&doc_id=20030904340293&doc_link_id=%2Fct4orabi%2F2003%2F004505%2F448%2F0332-0332%26dl%3D2&url=http%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fct4orabi%2F2003%2F004505%2F448%2F0332-0332%26dl%3D2&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_5.gif

記述言語：日本語   出版者・発行元：(公社)日本補綴歯科学会  

CiNii Article

CiNii Books

researchmap

J-GLOBAL

researchmap

記述言語：英語   出版者・発行元：(一社)日本解剖学会  

researchmap

researchmap

researchmap