Updated on 2025/07/01

写真a

 
KAKU Masaru
 
Organization
Academic Assembly Institute of Medicine and Dentistry SHIGAKU KEIRETU Associate Professor
Faculty of Dentistry Department of Dentistry Associate Professor
Graduate School of Medical and Dental Sciences Oral Life Science Oral Health Science Associate Professor
Title
Associate Professor
External link

Degree

  • 歯学博士（歯学） ( 2004.3   東京医科歯科大学 )

Research Areas

  • Life Science / Regenerative dentistry and dental engineering

  • Life Science / Oral biological science

  • Life Science / Prosthodontics

Research History (researchmap)

  • Niigata University

    2020.5

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  • University of Texas McGovern Medical School at Houston   Department of Pediatrics   Adjunct Associate Professor

    2017.5 - 2018.8

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  • Niigata University   Graduate School of Medical and Dental Sciences Oral Life Science Oral Health Science   Associate Professor

    2012.9

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  • Niigata University   Faculty of Dentistry School of Dentistry   Associate Professor

    2012.9

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  • Niigata University   University Medical and Dental Hospital Dental Health   Lecturer

    2010.12 - 2012.8

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  • Niigata University   University Medical and Dental Hospital Dental Health   Assistant Professor

    2009.4 - 2010.11

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  • University of North Carolina at Chapel Hill   Dental Research Center   Researcher

    2004.6 - 2009.3

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  • Tokyo Medical and Dental University   Graduate School of Medical and Dental Sciences

    2004.4 - 2008.3

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Research History

  • Niigata University   Graduate School of Medical and Dental Sciences Oral Life Science Oral Health Science   Associate Professor

    2012.9

  • Niigata University   Faculty of Dentistry School of Dentistry   Associate Professor

    2012.9

  • Niigata University   University Medical and Dental Hospital Dental Health   Lecturer

    2010.12 - 2012.8

  • Niigata University   University Medical and Dental Hospital Dental Health   Assistant Professor

    2009.4 - 2010.11

Education

  • Tokyo Medical and Dental University   医歯学総合研究科

    2000.4 - 2004.3

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    Country： Japan

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  • Tokyo Medical and Dental University   Faculty of Dentistry   歯学科

    1994.4 - 2000.3

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    Country： Japan

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Professional Memberships

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Qualification acquired

  • Dentist

 

Papers

  • Wnt/β-catenin Promotes Cementum Apposition in Periodontal Regeneration Reviewed

    Y. Ono, M. Kaku, L. Thant, H. Iwama, M. Arai, M. Mizukoshi, A. Dobashi, M. Kitami, M. M. Taketo, A. Ohazama, I. Saito, K. Uoshima

    Journal of Dental Research   104 ( 2 )   183 - 192   2025.2

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：SAGE Publications  

    DOI： 10.1177/00220345241286490

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    Other Link： https://journals.sagepub.com/doi/full-xml/10.1177/00220345241286490

  • A loss of primary cilia by a reduction in mTOR signaling correlates with age-related deteriorations in condylar cartilage Reviewed International journal

    Megumi Kitami, Masaru Kaku, Lay Thant, Takeyasu Maeda

    GeroScience   46 ( 6 )   5995 - 6007   2024.12

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s11357-024-01143-x

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  • descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques Reviewed International journal

    Kohei Otomo, Takaki Omura, Yuki Nozawa, Steven J. Edwards, Yukihiko Sato, Yuri Saito, Shigehiro Yagishita, Hitoshi Uchida, Yuki Watakabe, Kiyotada Naitou, Rin Yanai, Naruhiko Sahara, Satoshi Takagi, Ryohei Katayama, Yusuke Iwata, Toshiro Shiokawa, Yoku Hayakawa, Kensuke Otsuka, Haruko Watanabe-Takano, Yuka Haneda, Shigetomo Fukuhara, Miku Fujiwara, Takenobu Nii, Chikara Meno, Naoki Takeshita, Kenta Yashiro, Juan Marcelo Rosales Rocabado, Masaru Kaku, Tatsuya Yamada, Yumiko Oishi, Hiroyuki Koike, Yinglan Cheng, Keisuke Sekine, Jun Ichiro Koga, Kaori Sugiyama, Kenichi Kimura, Fuyuki Karube, Hyeree Kim, Ichiro Manabe, Tomomi Nemoto, Kazuki Tainaka, Akinobu Hamada, Hjalmar Brismar, Etsuo A. Susaki

    Nature Communications   15 ( 1 )   4941 - 4941   2024.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41467-024-49131-1

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  • 細胞外マトリックスのプロテオーム解析と歯周組織再生 Reviewed

    加来 賢

    BIO Clinica   39 ( 10 )   55 - 58   2024.9

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  • Acellular Extrinsic Fiber Cementum Is Invariably Present in the Superficial Layer of Apical Cementum in Mouse Molar. Reviewed International journal

    Hajime Iwama, Masaru Kaku, Lay Thant, Masaru Mizukoshi, Moe Arai, Yoshiki Ono, Kohei Kitami, Isao Saito, Katsumi Uoshima

    The journal of histochemistry and cytochemistry   72 ( 2 )   109 - 120   2024.2

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    The cementum is a highly mineralized tissue that covers the tooth root. The regional differences among the types of cementum, especially in the extrinsic fibers that contribute to tooth support, remain controversial. Therefore, this study used second harmonic generation imaging in conjunction with automated collagen extraction and image analysis algorithms to facilitate the quantitative examination of the fiber characteristics and the changes occurring in these fibers over time. Acellular extrinsic fiber cementum (AEFC) was invariably observed in the superficial layer of the apical cementum in mouse molars, indicating that this region of the cementum plays a crucial role in supporting the tooth. The apical AEFC exhibited continuity and fiber characteristics comparable with the cervical AEFC, suggesting a common cellular origin for their formation. The cellular intrinsic fiber cementum present in the inner layer of the apical cementum showed consistent growth in the apical direction without layering. This study highlights the dynamic nature of the cementum in mouse molars and underscores the requirement for re-examining its structure and roles. The findings of the present study elucidate the morphophysiological features of cementum and have broader implications for the maintenance of periodontal tissue health.

    DOI： 10.1369/00221554241229130

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  • Multiomics analysis of cultured mouse periodontal ligament cell-derived extracellular matrix Reviewed International journal

    Masaru Kaku, Lay Thant, Azusa Dobashi, Yoshiki Ono, Megumi Kitami, Masaru Mizukoshi, Moe Arai, Hajime Iwama, Kohei Kitami, Yoshito Kakihara, Masaki Matsumoto, Isao Saito, Katsumi Uoshima

    Scientific Reports   14 ( 1 )   354 - 354   2024.1

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-023-51054-8

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  • 歯根膜由来細胞シートにおける細胞外マトリックス Reviewed

    加来 賢, 魚島 勝美

    Precision Medicine   7 ( 1 )   51 - 54   2024.1

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    Authorship：Lead author,　Corresponding author   Language：Japanese   Publisher：(株)北隆館  

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  • Effect of Sparc knockout on the extracellular matrix of mouse periodontal ligament cells. Reviewed International journal

    Moe Arai, Masaru Kaku, Lay Thant, Megumi Kitami, Yoshiki Ono, Azusa Dobashi, Hajime Iwama, Masaru Mizukoshi, Kohei Kitami, Masaki Matsumoto, Isao Saito, Katsumi Uoshima

    Biochemical and biophysical research communications   692   149364 - 149364   2023.12

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    The periodontal ligament (PDL) is a critical component in maintaining tooth stability. It is composed of cells and an extracellular matrix (ECM), each with unique roles in tissue function and homeostasis. Secreted protein acidic and rich in cysteine (SPARC), a calcium-binding matricellular glycoprotein, plays a crucial role in regulating ECM assembly and turnover, alongside facilitating cellular-ECM interactions. In the present study, mass spectrometry-based proteomics was used to assess the impacts of Sparc-knockout (KO) on PDL-derived cells. Results demonstrated that Sparc-KO significantly reduces ECM production and alters its composition with increased levels of type I collagen. Despite this increase in Sparc-KO, type I collagen was not likely to be effectively integrated into the fibrils due to collagen cross-linking impairment. Furthermore, the pathway and process enrichment analyses suggested that SPARC plays a protective role against ECM degradation by antagonistically interacting with cell-surface collagen receptors. These findings provide detailed insights into the multifaceted role of SPARC in ECM organization, including its impact on ECM production, collagen regulation, and interactions with various cellular compartments. A better understanding of these complex mechanisms is crucial for comprehending the causes of periodontal disease and tissue regeneration, where precise control of ECM organization is necessary.

    DOI： 10.1016/j.bbrc.2023.149364

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  • Bundling of collagen fibrils influences osteocyte network formation during bone modeling Reviewed International journal

    Mana Hashimoto, Haruka Takahashi, Kaori Tabata-Okubo, Noriyuki Nagaoka, Kazuaki Tokunaga, Haruka Matsumori, Yoshihito Ishihara, Masaru Kaku, Tadahiro Iimura, Toru Hara, Hiroshi Kamioka

    Scientific Reports   13 ( 1 )   22028 - 22028   2023.12

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    Osteocytes form a cellular network by gap junctions between their cell processes. This network is important since intercellular communication via the network is essential for bone metabolism. However, the factors that influence the formation of this osteocyte network remain unknown. As the early stage of osteocyte network formation occurs on the bone surface, we observed a newly formed trabecular bone surface by orthogonal focused ion beam-scanning electron microscopy. The embedding late osteoblast processes tended to avoid bundled collagen fibrils and elongate into sparse collagen fibrils. Then, we examined whether the inhibition of bundling of collagen fibrils using a potent lysyl oxidase inhibitor, β-aminopropionitrile (BAPN) changed the cellular network of the chick calvaria. The osteocyte shape of the control group was spindle-shape, while that of the BAPN group was sphere-shaped. In addition, the osteocyte processes of the control group were elongated vertically to the long axis of the cell body, whereas the osteocyte processes of the BAPN group were elongated radially. Therefore, it was suggested that the bundling of collagen fibrils influences normal osteocyte network formation during bone modeling.

    DOI： 10.1038/s41598-023-48786-y

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  • Involvement of Rab11 in osteoblastic differentiation: Its up-regulation during the differentiation and by tensile stress. Reviewed International journal

    Lay Thant, Yoshito Kakihara, Masaru Kaku, Megumi Kitami, Kohei Kitami, Masaru Mizukoshi, Takeyasu Maeda, Isao Saito, Makio Saeki

    Biochemical and biophysical research communications   624   16 - 22   2022.10

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    Rab GTPases, the largest group of small monomeric GTPases, have been shown to participate in membrane trafficking involving many cellular processes. However, their roles during osteoblastic differentiation remain to be elucidated. In this study, we investigated Rab GTPase involvement in osteoblastic differentiation. Protein levels of a series of Rabs (Rab4, Rab5, Rab7, Rab9a, Rab11a/b, and Rab27) were increased during osteoblastic differentiation of MC3T3-E1 cells, and the Rab11a/b levels were particularly pronounced in the presence of Rho-associated coiled-coil-containing protein kinase (ROCK) inhibitor, an activator of osteoblastogenesis. We subsequently investigated the functional contribution of Rab11a and Rab11b during osteoblastic differentiation. The alkaline phosphatase (ALP) levels were reduced by Rab11b depletion but not by Rab11a depletion. Because our result suggested that Rab11a and Rab11b could be regulated downstream of Runx2 (Runt-related transcription factor 2), a key transcription factor for osteoblastic differentiation, we investigated the effects of the double knockdown of Runx2 and Rab11a or Rab11b on osteoblastic phenotypes. The double knockdown significantly reduced ALP activity as well as collagen deposition compared with single Runx2 knockdown. Furthermore, the Rab11a and Rab11b response to mechanical stress in vivo was investigated using a mouse orthodontic tooth movement model. Rab11a and Rab11b expression was enhanced in the periodontal ligament, where bone formation is activated by tensile stress. This study shows that Rab11a and Rab11b are regulated downstream of Runx2 in osteoblastic differentiation, and their expressions are also controlled by tensile stress.

    DOI： 10.1016/j.bbrc.2022.07.061

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  • Extracellular Matrix-Oriented Proteomic Analysis of Periodontal Ligament Under Mechanical Stress Reviewed International journal

    Lay Thant, Masaru Kaku, Yoshito Kakihara, Masaru Mizukoshi, Megumi Kitami, Moe Arai, Kohei Kitami, Daiki Kobayashi, Yutaka Yoshida, Takeyasu Maeda, Isao Saito, Katsumi Uoshima, Makio Saeki

    Frontiers in Physiology   13   899699 - 899699   2022.5

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    The periodontal ligament (PDL) is a specialized connective tissue that provides structural support to the tooth and is crucial for oral functions. The mechanical properties of the PDL are mainly derived from the tissue-specific composition and structural characteristics of the extracellular matrix (ECM). The ECM also plays key roles in determining cell fate in the cellular microenvironment thus crucial in the PDL tissue homeostasis. In the present study, we determined the comprehensive ECM profile of mouse molar PDL using laser microdissection and mass spectrometry-based proteomic analysis with ECM-oriented data curation. Additionally, we evaluated changes in the ECM proteome under mechanical loading using a mouse orthodontic tooth movement (OTM) model and analyzed potential regulatory networks using a bioinformatics approach. Proteomic changes were evaluated in reference to the novel second harmonic generation (SHG)-based fiber characterization. Our ECM-oriented proteomics approach succeeded in illustrating the comprehensive ECM profile of the mouse molar PDL. We revealed the presence of type II collagen in PDL, possibly associated with the load-bearing function upon occlusal force. Mechanical loading induced unique architectural changes in collagen fibers along with dynamic compositional changes in the matrisome profile, particularly involving ECM glycoproteins and matrisome-associated proteins. We identified several unique matrisome proteins which responded to the different modes of mechanical loading in PDL. Notably, the proportion of type VI collagen significantly increased at the mesial side, contributing to collagen fibrogenesis. On the other hand, type XII collagen increased at the PDL-cementum boundary of the distal side. Furthermore, a multifaceted bioinformatics approach illustrated the potential molecular cues, including PDGF signaling, that maintain ECM homeostasis under mechanical loading. Our findings provide fundamental insights into the molecular network underlying ECM homeostasis in PDL, which is vital for clinical diagnosis and development of biomimetic tissue-regeneration strategies.

    DOI： 10.3389/fphys.2022.899699

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  • The academic value of JPR is steadily increasing.

    Masaru Kaku

    Journal of prosthodontic research   66 ( 4 )   vi-vii   2022

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    DOI： 10.2186/jpr.JPR_66_4_ed

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  • In vivo cell proliferation analysis and cell-tracing reveal the global cellular dynamics of periodontal ligament cells under mechanical-loading. Reviewed International journal

    Masaru Mizukoshi, Masaru Kaku, Lay Thant, Kohei Kitami, Moe Arai, Isao Saito, Katsumi Uoshima

    Scientific reports   11 ( 1 )   9813 - 9813   2021.5

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Periodontal ligament (PDL) is a uniquely differentiated tissue that anchors the tooth to the alveolar bone socket and plays key roles in oral function. PDL cells can respond rapidly to mechanical stimuli, resulting in accelerated tissue remodeling. Cell proliferation is an initial event in tissue remodeling and participates in maintaining the cell supply; therefore, analyzing cell-proliferative activity might provide a comprehensive view of cellular dynamics at the tissue level. In this study, we investigated proliferating cells in mouse molar PDL during orthodontic tooth movement (OTM)-induced tissue remodeling. Our results demonstrated that the mechanical stimuli evoked a dynamic change in the proliferative-cell profile at the entire PDL. Additionally, cell-tracing analysis revealed that the proliferated cells underwent further division and subsequently contributed to tissue remodeling. Moreover, OTM-induced proliferating cells expressed various molecular markers that most likely arise from a wide range of cell types, indicating the lineage plasticity of PDL cells in vivo. Although further studies are required, these findings partially elucidated the global views of the cell trajectory in mouse molar PDL under mechanical-loading conditions, which is vital for understanding the cellular dynamics of the PDL and beneficial for dental treatment in humans.

    DOI： 10.1038/s41598-021-89156-w

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  • Molecular Cloning of Mouse Homologue of Enamel Protein C4orf26 and Its Phosphorylation by FAM20C. Reviewed International journal

    Nattanan Govitvattana, Masaru Kaku, Yoshio Ohyama, Haytham Jaha, I-Ping Lin, Hanna Mochida, Prasit Pavasant, Yoshiyuki Mochida

    Calcified tissue international   109 ( 4 )   445 - 454   2021.4

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    It is widely accepted that cellular processes are controlled by protein phosphorylation and has become increasingly clear that protein degradation, localization and conformation as well as protein-protein interaction are the examples of subsequent cellular events modulated by protein phosphorylation. Enamel matrix proteins belong to members of the secretory calcium binding phosphoprotein (SCPP) family clustered on chromosome 4q21, and most of the SCPP phosphoproteins have at least one S-X-E motifs (S; serine, X; any amino acid, E; glutamic acid). It has been reported that mutations in C4orf26 gene, located on chromosome 4q21, are associated with autosomal recessive type of Amelogenesis Imperfecta (AI), a hereditary condition that affects enamel formation/mineralization. The enamel phenotype observed in patients with C4orf26 mutations is hypomineralized and partially hypoplastic, indicating that C4orf26 protein may function at both secretory and maturation stages of amelogenesis. The previous in vitro study showed that the synthetic phosphorylated peptide based on C4orf26 protein sequence accelerates hydroxyapatite nucleation. Here we show the molecular cloning of Gm1045, mouse homologue of C4orf26, which has 2 splicing isoforms. Immunohistochemical analysis demonstrated that the immunolocalization of Gm1045 is mainly observed in enamel matrix in vivo. Our report is the first to show that FAM20C, the Golgi casein kinase, phosphorylates C4orf26 and Gm1045 in cell cultures. The extracellular localization of C4orf26/Gm1045 was regulated by FAM20C kinase activity. Thus, our data point out the biological importance of enamel matrix-kinase control of SCPP phosphoproteins and may have a broad impact on the regulation of amelogenesis and AI.

    DOI： 10.1007/s00223-021-00847-y

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  • FAM20C directly binds to and phosphorylates Periostin. Reviewed International journal

    Ju-Hsien Lin, I-Ping Lin, Yoshio Ohyama, Hanna Mochida, Akira Kudo, Masaru Kaku, Yoshiyuki Mochida

    Scientific reports   10 ( 1 )   17155 - 17155   2020.10

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    It is widely accepted that FAM20C functions as a Golgi casein kinase and has large numbers of kinase substrates within the secretory pathway. It has been previously reported that FAM20C is required for maintenance of healthy periodontal tissues. However, there has been no report that any extracellular matrix molecules expressed in periodontal tissues are indeed substrates of FAM20C. In this study, we sought to identify the binding partner(s) of FAM20C. FAM20C wild-type (WT) and its kinase inactive form D478A proteins were generated. These proteins were electrophoresed and the Coomassie Brilliant Blue (CBB)-positive bands were analyzed to identify FAM20C-binding protein(s) by Mass Spectrometry (MS) analysis. Periostin was found by the analysis and the binding between FAM20C and Periostin was investigated in cell cultures and in vitro. We further determined the binding region(s) within Periostin responsible for FAM20C-binding. Immunolocalization of FAM20C and Periostin was examined using mouse periodontium tissues by immunohistochemical analysis. In vitro kinase assay was performed using Periostin and FAM20C proteins to see whether FAM20C phosphorylates Periostin in vitro. We identified Periostin as one of FAM20C-binding proteins by MS analysis. Periostin interacted with FAM20C in a kinase-activity independent manner and the binding was direct in vitro. We further identified the binding domain of FAM20C in Periostin, which was mapped within Fasciclin (Fas) I domain 1-4 of Periostin. Immunolocalization of FAM20C was observed in periodontal ligament (PDL) extracellular matrix where that of Periostin was also immunostained in murine periodontal tissues. FAM20C WT, but not D478A, phosphorylated Periostin in vitro. Consistent with the overlapped expression pattern of FAM20C and Periostin, our data demonstrate for the first time that Periostin is a direct FAM20C-binding partner and that FAM20C phosphorylates Periostin in vitro.

    DOI： 10.1038/s41598-020-74400-6

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  • 咬合支持域の減少による咀嚼障害をインプラントにて補綴した症例 Reviewed

    加来 賢

    日本補綴歯科学会誌   12 ( 1 )   103 - 106   2020.1

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  • Response to Letter to the Editor: Concerns on modeling postmenopausal osteoporosis in young female rats. Reviewed International journal

    Juan Marcelo Rosales Rocabado, Masaru Kaku, Kosuke Nozaki, Takako Ida, Megumi Kitami, Yujin Aoyagi, Katsumi Uoshima

    Journal of orthopaedic surgery and research   14 ( 1 )   451 - 451   2019.12

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    Authorship：Corresponding author   Language：English  

    DOI： 10.1186/s13018-019-1485-2

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  • Collagen Crosslinks and Bone Metabolism Involved in Osseointegration Invited Reviewed

    KAKU Masaru

    32 ( 2 )   26 - 32   2019.6

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  • Valproic Acid contribute to Bone Cavity Healing in Rats. Reviewed

    Rashid M, Akiba Y, Eguchi K Akiba N, Kaku M, Nagasawa M, Uoshima K

    Dentistry   3   539 - 546   2019.4

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  • IFT20 is required for the maintenance of cartilaginous matrix in condylar cartilage. Reviewed International journal

    Megumi Kitami, Hiroyuki Yamaguchi, Masayuki Ebina, Masaru Kaku, Di Chen, Yoshihiro Komatsu

    Biochemical and biophysical research communications   509 ( 1 )   222 - 226   2019.1

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    DOI： 10.1016/j.bbrc.2018.12.107

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  • Regeneration of Bone/ Periodontal ligament and Mechanical load ± ([SORULQJ WKH VLJQL¿FDQFH LQ 3URVWKRGRQWLF 7UHDWPHQWV ± Reviewed

    Katsumi Uoshima, Masaru Kaku, Masako Nagasawa

    Ann Jpn Prosthodont Soc   11 ( 1 )   14 - 19   2019.1

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  • A multi-factorial analysis of bone morphology and fracture strength of rat femur in response to ovariectomy. Reviewed International journal

    Juan Marcelo Rosales Rocabado, Masaru Kaku, Kosuke Nozaki, Takako Ida, Megumi Kitami, Yujin Aoyagi, Katsumi Uoshima

    Journal of orthopaedic surgery and research   13 ( 1 )   318 - 318   2018.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s13018-018-1018-4

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  • VWC2 Increases Bone Formation Through Inhibiting Activin Signaling. Reviewed International journal

    Ahmad Almehmadi, Yoshio Ohyama, Masaru Kaku, Ahmed Alamoudi, Dina Husein, Michitsuna Katafuchi, Yuji Mishina, Yoshiyuki Mochida

    Calcified tissue international   103 ( 6 )   663 - 674   2018.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00223-018-0462-9

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  • BRCA1 and BRCA2 tumor suppressors in neural crest cells are essential for craniofacial bone development. Reviewed International journal

    Kohei Kitami, Megumi Kitami, Masaru Kaku, Bin Wang, Yoshihiro Komatsu

    PLoS genetics   14 ( 5 )   e1007340   2018.5

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    DOI： 10.1371/journal.pgen.1007340

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  • A paradigm shift for bone quality in prosthetic dentistry Reviewed

    Kuroshima Shinichiro, Kaku Masaru, Ishimoto Takuya, Sasaki Muneteru, Nakano Takayoshi, Sawase Takashi

    Annals of Japan Prosthodontic Society   10 ( 1 )   1 - 15   2018.1

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Prosthodontic Society  

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  • Extracellular matrix with defective collagen cross-linking affects the differentiation of bone cells. Reviewed International journal

    Takako Ida, Masaru Kaku, Megumi Kitami, Masahiko Terajima, Juan Marcelo Rosales Rocabado, Yosuke Akiba, Masako Nagasawa, Mitsuo Yamauchi, Katsumi Uoshima

    PloS one   13 ( 9 )   e0204306   2018

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0204306

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  • A paradigm shift for bone quality in dentistry: A literature review. Reviewed International journal

    Shinichiro Kuroshima, Masaru Kaku, Takuya Ishimoto, Muneteru Sasaki, Takayoshi Nakano, Takashi Sawase

    Journal of prosthodontic research   61 ( 4 )   353 - 362   2017.10

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    DOI： 10.1016/j.jpor.2017.05.006

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  • An ENU-induced splice site mutation of mouse Col1a1 causing recessive osteogenesis imperfecta and revealing a novel splicing rescue. Reviewed International journal

    Koichi Tabeta, Xin Du, Kei Arimatsu, Mai Yokoji, Naoki Takahashi, Norio Amizuka, Tomoka Hasegawa, Karine Crozat, Tomoki Maekawa, Sayuri Miyauchi, Yumi Matsuda, Takako Ida, Masaru Kaku, Kasper Hoebe, Kinji Ohno, Hiromasa Yoshie, Kazuhisa Yamazaki, Eva Marie Y Moresco, Bruce Beutler

    Scientific reports   7 ( 1 )   11717 - 11717   2017.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-017-10343-9

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  • Recruitment of bone marrow-derived cells to the periodontal ligament via the stromal cell-derived factor-1/C-X-C chemokine receptor type 4 axis Reviewed

    M. Kaku, M. Kitami, J. M. Rosales Rocabado, T. Ida, Y. Akiba, K. Uoshima

    Journal of Periodontal Research   52 ( 4 )   686 - 694   2017.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/jre.12433

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  • Functional Diversity of Ciliary Proteins in Bone Development and Disease. Reviewed International journal

    Masaru Kaku, Yoshihiro Komatsu

    Current osteoporosis reports   15 ( 2 )   96 - 102   2017.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s11914-017-0351-6

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  • Medication-related osteonecrosis of the jaw; what should we do as prosthodontists? Reviewed International journal

    Shinichiro Kuroshima, Masaru Kaku, Takashi Matsuura, Ikiru Atsuta, Yasunori Ayukawa, Takashi Sawase

    Journal of prosthodontic research   60 ( 4 )   229 - 230   2016.10

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  • Prolonged Survival of Transplanted Osteoblastic Cells Does Not Directly Accelerate the Healing of Calvarial Bone Defects. Reviewed International journal

    Megumi Kitami, Masaru Kaku, Juan Marcelo Rosales Rocabado, Takako Ida, Nami Akiba, Katsumi Uoshima

    Journal of cellular physiology   231 ( 9 )   1974 - 82   2016.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jcp.25302

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  • Prosthodontics: A multidisciplinary field in dentistry. Reviewed International journal

    Masaru Kaku

    Journal of prosthodontic research   60 ( 3 )   143 - 4   2016.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jpor.2016.05.001

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  • FAM20A binds to and regulates FAM20C localization. Reviewed International journal

    Yoshio Ohyama, Ju-Hsien Lin, Nattanan Govitvattana, I-Ping Lin, Sundharamani Venkitapathi, Ahmed Alamoudi, Dina Husein, Chunying An, Hak Hotta, Masaru Kaku, Yoshiyuki Mochida

    Scientific reports   6   27784 - 27784   2016.6

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    DOI： 10.1038/srep27784

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  • Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development. Reviewed International journal

    Kazuo Noda, Megumi Kitami, Kohei Kitami, Masaru Kaku, Yoshihiro Komatsu

    Proceedings of the National Academy of Sciences of the United States of America   113 ( 19 )   E2589-97 - E2597   2016.5

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    DOI： 10.1073/pnas.1519458113

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  • Mechanical Loading Stimulates Expression of Collagen Cross-Linking Associated Enzymes in Periodontal Ligament. Reviewed International journal

    Masaru Kaku, Juan Marcelo Rosales Rocabado, Megumi Kitami, Takako Ida, Yosuke Akiba, Mitsuo Yamauchi, Katsumi Uoshima

    Journal of cellular physiology   231 ( 4 )   926 - 33   2016.4

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    DOI： 10.1002/jcp.25184

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  • Correlation Between Stress Distributions and Biological Reactions in Bone Surrounding Implants That Support Cantilevers in Supraocclusal Contact in Rats. Reviewed International journal

    Ryohei Takano, Masako Nagasawa, Megumi Kitami, Juan Marcelo Rosales Rocabado, Masaru Kaku, Roxana Stegaroiu, Katsumi Uoshima

    Implant dentistry   25 ( 2 )   204 - 13   2016.4

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    DOI： 10.1097/ID.0000000000000369

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  • Immunohistochemical localization of tenascin-C in rat periodontal ligament with reference to alveolar bone remodeling. Reviewed

    Rei Sato, Hiroki Fukuoka, Tamaki Yokohama-Tamaki, Masaru Kaku, Shunichi Shibata

    Anatomical science international   91 ( 2 )   196 - 206   2016.3

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    DOI： 10.1007/s12565-015-0285-y

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  • Cell-based bone regeneration for alveolar ridge augmentation--cell source, endogenous cell recruitment and immunomodulatory function. Reviewed International journal

    Masaru Kaku, Yosuke Akiba, Kentaro Akiyama, Daisuke Akita, Masahiro Nishimura

    Journal of prosthodontic research   59 ( 2 )   96 - 112   2015.4

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    DOI： 10.1016/j.jpor.2015.02.001

    Web of Science

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  • Analysis of Patients Visiting Niigata University Medical and Dental Hospital with Chief Complaints of Metal Allergy and/or Focal Infection in the Previous 8 Years Reviewed

    Akiba Y, Tomizuka K, Kaku M, Kawasaki M, Nagasawa M, Takano R, Uoshima K.

    The Indonesian Journal of Dental Research   1 ( 2 )   2015

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  • Mechano-regulation of collagen biosynthesis in periodontal ligament. Reviewed International journal

    Masaru Kaku, Mitsuo Yamauchi

    Journal of prosthodontic research   58 ( 4 )   193 - 207   2014.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jpor.2014.08.003

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  • ヒストン脱アセチル化酵素阻害剤(HDACI)を用いたエピジェネティクス制御による細胞分化制御を利用した新規骨増成法に関する研究

    秋葉 陽介, 江口 香里, Rashid Md. Mamunur, 加来 賢, 秋葉 奈美, 魚島 勝美

    日本歯科医学会誌   33   44 - 48   2014.3

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  • Royal jelly affects collagen crosslinking in bone of ovariectomized rats Reviewed

    Masaru Kaku, Juan Marcelo Rosales Rocabado, Megumi Kitami, Takako Ida, Katsumi Uoshima

    Journal of Functional Foods   7 ( 1 )   398 - 406   2014

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    DOI： 10.1016/j.jff.2014.01.019

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  • Osteoblastic differentiation and mineralization ability of periosteum-derived cells compared with bone marrow and calvaria-derived cells Reviewed

    Rosales-Rocabado, J. M., Kaku, M., Kitami, M., Akiba, Y., Uoshima, K.

    J Oral Maxillofac Surg   72 ( 4 )   694.e1 - 694.e9   2014

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    DOI： 10.1016/j.joms.2013.12.001

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  • 【力を診る-歯列を守る力のマネジメント-】力が加わったら生体はどう変化するか 生体力学と力の生物学 歯根膜のメカノバイオロジー Reviewed

    魚島 勝美, 加来 賢

    補綴臨床   別冊 ( 力を診る-歯列を守る力のマネジメント- )   46 - 51   2012.12

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  • Identification and characterization of neural crest-derived cells in adult periodontal ligament of mice Reviewed

    Kaku, M., Komatsu, Y., Mochida, Y., Yamauchi, M., Mishina, Y., Ko, C. C.

    Arch Oral Biol   57 ( 12 )   1668 - 1675   2012

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    DOI： 10.1016/j.archoralbio.2012.04.022

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  • Distribution and relative activity of matrix metalloproteinase-2 in human coronal dentin. Reviewed International journal

    Lee W Boushell, Masaru Kaku, Yoshiyuki Mochida, Mitsuo Yamauchi

    International journal of oral science   3 ( 4 )   192 - 9   2011.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.4248/IJOS11070

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  • Podocan-like protein: a novel small leucine-rich repeat matrix protein in bone. Reviewed International journal

    Yoshiyuki Mochida, Masaru Kaku, Keiko Yoshida, Michitsuna Katafuchi, Phimon Atsawasuwan, Mitsuo Yamauchi

    Biochemical and biophysical research communications   410 ( 2 )   333 - 8   2011.7

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    DOI： 10.1016/j.bbrc.2011.05.150

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  • Effect of cyclic mechanical loading on osteoclast recruitment in periodontal tissue Reviewed

    K. Nozaki, M. Kaku, Y. Yamashita, M. Yamauchi, H. Miura

    Journal of Periodontal Research   45 ( 1 )   8 - 15   2010.2

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    DOI： 10.1111/j.1600-0765.2008.01193.x

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  • Lysyl oxidase binds transforming growth factor-beta and regulates its signaling via amine oxidase activity. Reviewed International journal

    Phimon Atsawasuwan, Yoshiyuki Mochida, Michitsuna Katafuchi, Masaru Kaku, Keith S K Fong, Katalin Csiszar, Mitsuo Yamauchi

    The Journal of biological chemistry   283 ( 49 )   34229 - 40   2008.12

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    DOI： 10.1074/jbc.M803142200

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  • Immunohistochemical localization of matrixmetalloproteinase-2 in human coronal dentin. Reviewed International journal

    Lee W Boushell, Masaru Kaku, Yoshiyuki Mochida, Robert Bagnell, Mitsuo Yamauchi

    Archives of oral biology   53 ( 2 )   109 - 16   2008.2

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    DOI： 10.1016/j.archoralbio.2007.09.012

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  • 1,25(OH)2D3 regulates collagen quality in an osteoblastic cell culture system Reviewed

    Nagaoka, H., Mochida, Y., Atsawasuwan, P., Kaku, M., Kondoh, T., Yamauchi, M.

    Biochem Biophys Res Commun   377 ( 2 )   674 - 678   2008

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    DOI： 10.1016/j.bbrc.2008.10.036

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  • The effect of hydroxyapatite/gemosil nanocomposites on extracellular matrix patterning through the promotion of osteoblast self-organization Reviewed

    Ching Chang Ko, Alice Ma, Masaru Kaku, Kai Li Liu, T. J.M. Luo, J. F.C. Tulloch, W. S. Hu

    8th World Biomaterials Congress 2008   2   611   2008

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  • Post-translational modifications of collagen upon BMP-induced osteoblast differentiation. Reviewed International journal

    Masaru Kaku, Yoshiyuki Mochida, Phimon Atsawasuwan, Duenpim Parisuthiman, Mitsuo Yamauchi

    Biochemical and biophysical research communications   359 ( 3 )   463 - 8   2007.8

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    DOI： 10.1016/j.bbrc.2007.05.109

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  • Effects of formalin fixation and collagen cross-linking on T2 and magnetization transfer in bovine nasal cartilage. Reviewed International journal

    Kenneth W Fishbein, Yehezkiel A Gluzband, Masaru Kaku, Hasina Ambia-Sobhan, Sue A Shapses, Mitsuo Yamauchi, Richard G Spencer

    Magnetic resonance in medicine   57 ( 6 )   1000 - 11   2007.6

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    DOI： 10.1002/mrm.21216

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  • Nephrocan, a novel member of the small leucine-rich repeat protein family, is an inhibitor of transforming growth factor-beta signaling. Reviewed International journal

    Yoshiyuki Mochida, Duenpim Parisuthiman, Masaru Kaku, Jun-ichi Hanai, Vikas P Sukhatme, Mitsuo Yamauchi

    The Journal of biological chemistry   281 ( 47 )   36044 - 51   2006.11

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    DOI： 10.1074/jbc.M604787200

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  • Investigation of periodontal ligament reaction upon excessive occlusal load--osteopontin induction among periodontal ligament cells. Reviewed International journal

    Masaru Kaku, Katsumi Uoshima, Yasuo Yamashita, Hiroyuki Miura

    Journal of periodontal research   40 ( 1 )   59 - 66   2005.2

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    DOI： 10.1111/j.1600-0765.2004.00773.x

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▶ display all

Books

  • 新・力を診る : 臨床と研究の接点

    （ Role： Contributor ,  歯根膜のメカノバイオロジー（魚島勝美，加来 賢））

    医歯薬出版  2024.1  （ ISBN:9784263461778 ）

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    Total pages：217p   Language：Japanese

    CiNii Books

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MISC

  • マウス臼歯根尖側の最表層には外部性線維性セメント質が存在する

    岩間 基, 加来 賢, Thant Lay, 新井 萌生, 水越 優, 北見 公平, 魚島 勝美, 齋藤 功

    新潟歯学会雑誌   53 ( 2 )   97 - 97   2024.1

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  • Periostin knockout歯根膜細胞における細胞外マトリックスの解析

    土橋 梓, 加来 賢, Thant Lay, 小林 水輝, 小野 喜樹, 魚島 勝美

    新潟歯学会雑誌   53 ( 2 )   99 - 99   2024.1

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  • Consideration of quantitative evaluation of tooth carving products.

    江口香里, 加来賢, ROSALES Juan Marcelo, 秋葉陽介, 秋葉奈美, 魚島勝美

    日本補綴歯科学会誌(Web)   16   2024

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  • 骨芽細胞の分化過程における細胞外マトリックスの組成変化 プロテオームによる網羅的定量解析(Changes in extracellular matrix composition during osteoblast differentiation: Comprehensive quantitative analysis by proteomics)

    ライン・プィン・ピュ, 加来 賢, レイ・タント, 土橋 梓, 小林 水輝, 小野 喜樹, 魚島 勝美

    日本補綴歯科学会誌   15 ( 関越支部学術大会特別号 )   20 - 20   2023.11

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  • マウス歯根膜細胞における主要なLamininの同定とその機能解析

    小林 水輝, 加来 賢, 土橋 梓, Thant Lay, 小野 喜樹, 魚島 勝美

    日本結合組織学会学術大会プログラム・抄録集   55回   127 - 127   2023.6

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  • 化学的消化法によりECMの可溶化効率を向上させた培養歯根膜細胞と歯根膜組織のプロテオーム解析

    土橋 梓, 加来 賢, Thant Lay, 小野 喜樹, 小林 水輝, 魚島 勝美

    日本結合組織学会学術大会プログラム・抄録集   55回   130 - 130   2023.6

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  • 培養歯根膜細胞における細胞外マトリックスのマルチオミックス解析

    土橋 梓, 加来 賢, Thant Lay, 小林 水輝, 小野 喜樹, 魚島 勝美

    日本補綴歯科学会誌   15 ( 特別号 )   263 - 263   2023.5

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  • マウス歯根膜細胞の主要LamininであるLaminin-411は細胞接着を促進させる

    小林 水輝, 加来 賢, 土橋 梓, Thant Lay, 小野 喜樹, 魚島 勝美

    日本補綴歯科学会誌   15 ( 特別号 )   264 - 264   2023.5

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  • Wnt/β-cateninシグナルは歯周組織再生に寄与する

    小野 喜樹, 加来 賢, 土橋 梓, 小林 水輝, 魚島 勝美

    日本補綴歯科学会誌   15 ( 特別号 )   268 - 268   2023.5

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  • SPARCの欠失が歯根膜細胞の線維形成に及ぼす影響のプロテオーム解析

    新井 萌生, 加来 賢, Lay Thant, 土橋 梓, 岩間 基, 水越 優, 北見 公平, 魚島 勝美, 齋藤 功

    新潟歯学会雑誌   52 ( 2 )   93 - 93   2022.12

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  • 歯周組織再生過程におけるWnt/β-cateninシグナルの亢進はセメント質の形成を促進する

    小野 喜樹, 加来 賢, 土橋 梓, 魚島 勝美

    新潟歯学会雑誌   52 ( 2 )   96 - 96   2022.12

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  • 加齢によりマウス臼歯の根尖側セメント質表層に生じる無細胞セメント質様組織の解析

    岩間 基, 加来 賢, Thant Lay, 新井 萌生, 水越 優, 北見 公平, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   81回   167 - 167   2022.10

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  • 培養歯根膜細胞から分泌された細胞外マトリックスのプロテオーム解析

    新井 萌生, 加来 賢, Thant Lay, 岩間 基, 水越 優, 北見 公平, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   81回   163 - 163   2022.10

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  • 加齢によりマウス臼歯の根尖側セメント質表層に生じる無細胞セメント質様組織の解析

    岩間 基, 加来 賢, Thant Lay, 新井 萌生, 水越 優, 北見 公平, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   81回   167 - 167   2022.10

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  • 培養歯根膜細胞から分泌された細胞外マトリックスのプロテオーム解析

    新井 萌生, 加来 賢, Thant Lay, 岩間 基, 水越 優, 北見 公平, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   81回   163 - 163   2022.10

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  • プライマリーシリアを介した下顎頭軟骨老化メカニズムの探索(Exploration of mandibular condylar chondrocytes aging mechanism by primary cilia)

    北見 恩美, 加来 賢

    Journal of Oral Biosciences Supplement   2022   352 - 352   2022.9

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  • Periostin knockout歯根膜細胞のマルチオミックス解析

    土橋 梓, 加来 賢, 小野 喜樹, 魚島 勝美

    日本補綴歯科学会誌   14 ( 特別号 )   150 - 150   2022.7

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  • Wnt/β-cateninシグナルが歯根膜細胞によるセメント質形成に及ぼす影響

    小野 喜樹, 加来 賢, 土橋 梓, 魚島 勝美

    日本補綴歯科学会誌   14 ( 特別号 )   281 - 281   2022.7

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  • Wnt/β-cateninシグナルの亢進が歯周組織再生に及ぼす影響

    小野 喜樹, 加来 賢, 土橋 梓, 魚島 勝美

    日本結合組織学会学術大会プログラム・抄録集   54回   128 - 128   2022.6

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  • Periostin knockout歯根膜細胞における細胞外基質のオミックス解析

    土橋 梓, 加来 賢, Thant Lay, 小野 喜樹, 魚島 勝美

    日本結合組織学会学術大会プログラム・抄録集   54回   120 - 120   2022.6

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  • 創傷治癒に伴うセメント質添加過程におけるβ-catenin発現細胞の局在

    小野 喜樹, 加来 賢, 土橋 梓, 魚島 勝美

    日本口腔インプラント学会誌   34 ( 特別号 )   ポスター - 35   2021.12

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  • 力学的刺激に対する歯根膜の耐性獲得に関わる分子ネットワークの解析

    土橋 梓, 加来 賢, Rocabado Juan Marcelo Rosales, 小野 喜樹, 魚島 勝美

    日本補綴歯科学会誌   13 ( 関越支部学術大会特別号 )   13 - 13   2021.11

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  • マウス臼歯歯根膜におけるLabel Retaining Cellの局在と特性

    水越 優, 加来 賢, 北見 公平, 新井 萌生, 岩間 基, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   80回   151 - 151   2021.11

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  • 矯正的歯の移動時の歯根膜におけるSPARCの局在について

    新井 萌生, 北見 公平, 加来 賢, 水越 優, Thant Lay, 岩間 基, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   80回   148 - 148   2021.11

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  • 加齢によりマウス臼歯の有細胞セメント質表層に生じる無細胞セメント質様組織の解析

    岩間 基, 北見 公平, 加来 賢, Thant Lay, 新井 萌生, 水越 優, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   80回   147 - 147   2021.11

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  • 歯根膜の組織修復過程におけるWnt/β-cateninシグナルの解析

    小野 喜樹, 加来 賢, 土橋 梓, 魚島 勝美

    日本補綴歯科学会誌   13 ( 関越支部学術大会特別号 )   13 - 13   2021.11

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  • 先端テクノロジーが拓く2040年の補綴治療～バックキャスティング思考によるイノベーション戦略～ メカニカルストレスの制御を基盤とした歯科補綴治療戦略

    加来 賢, 魚島 勝美

    日本歯科医師会雑誌   74 ( 4 )   341 - 341   2021.7

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  • メカニカルストレス下における歯根膜細胞外基質タンパクのプロテオーム解析

    Thant Lay, 加来 賢, 柿原 嘉人, 水越 優, 北見 恩美, 北見 公平, 小林 大記, 吉田 豊, 魚島 勝美, 齋藤 功, 佐伯 万騎男

    日本結合組織学会学術大会プログラム・抄録集   53回   137 - 137   2021.6

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  • Prosthodontics as biological science-mesenchymal stem cell perspective-

    熱田生, 秋山謙太郎, 加来賢

    日本補綴歯科学会誌(Web)   13   2021

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  • 矯正的歯の移動時における歯根膜増殖期細胞の特性

    水越 優, 加来 賢, 北見 公平, 新井 萌生, 井田 貴子, 魚島 勝美, 齋藤 功

    新潟歯学会雑誌   49 ( 2 )   84 - 84   2019.12

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  • 矯正学的歯の移動時における一次繊毛を有する歯根膜細胞の局在

    北見 公平, 水越 優, 新井 萌生, 加来 賢, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   78回   179 - 179   2019.11

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  • 矯正的歯の移動時のマウス歯根膜における増殖期細胞の局在と特性

    水越 優, 加来 賢, 北見 公平, 井田 貴子, 新井 萌生, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   78回   172 - 172   2019.11

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  • 本学歯学部学生の歯冠修復学実習における支台歯形成自己評価能力について

    長澤麻沙子, 秋葉奈美, 秋葉陽介, 加来賢, 青柳裕仁, ROCABADO J, M Rosales, 江口香里, 魚島勝美

    日本歯科医学教育学会総会・学術大会プログラム・抄録集   38回   94 - 94   2019.6

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  • 【海外の基礎研究はインプラント治療をどう変えたか?:From Bench to Clinic】オッセオインテグレーションの獲得に関わる骨代謝とコラーゲン架橋

    加来 賢, 井田 貴子, 長澤 麻沙子, 魚島 勝美

    日本口腔インプラント学会誌   32 ( 2 )   108 - 115   2019.6

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  • 歯根膜発生過程における細胞周期動態のin vivo解析

    井田 貴子, 加来 賢, 水越 優, 北見 公平, 魚島 勝美

    日本補綴歯科学会誌   11 ( 特別号 )   250 - 250   2019.5

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  • Regeneration of Bone/ Periodontal ligament and Mechanical load - Exploring the significance in Prosthodontic Treatments -

    魚島勝美, 加来賢, 長澤麻沙子

    日本補綴歯科学会誌(Web)   11 ( 1 )   14‐19(J‐STAGE)   2019

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  • 矯正的歯の移動時のマウス歯根膜における増殖/静止期細胞の局在

    水越 優, 加来 賢, 北見 公平, 井田 貴子, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   77回   231 - 231   2018.10

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  • 海外の基礎研究はインプラント治療をどう変えたか? From Bench to Clinic 骨質の概念をコラーゲンの生合成から理解する

    加来 賢

    日本口腔インプラント学会学術大会抄録集   48回   342 - 342   2018.9

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  • A paradigm shift for bone quality in prosthetic dentistry

    黒嶋伸一郎, 黒嶋伸一郎, 加来賢, 加来賢, 石本卓也, 佐々木宗輝, 中野貴由, 澤瀬隆

    日本補綴歯科学会誌(Web)   10 ( 1 )   2018

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  • がん抑制遺伝子BRCA1は顎顔面骨の形態形成に必須である

    北見 公平, 北見 恩美, 加来 賢, 小松 義広, 齋藤 功

    新潟歯学会雑誌   46 ( 2 )   112 - 113   2016.12

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  • マウス頭蓋骨発生におけるIFT20の役割

    野田 和男, 北見 恩美, 北見 公平, 加来 賢, 小松 義広

    日本骨代謝学会学術集会プログラム抄録集   34回   193 - 193   2016.7

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  • SDF-1/CXCR4による歯根膜への骨髄由来細胞の誘導

    加来 賢, 北見 恩美, Rosales Rocavado J.M., 井田 貴子, 秋葉 陽介, 魚島 勝美

    日本補綴歯科学会誌   8 ( 特別号 )   237 - 237   2016.7

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  • 骨質研究がもたらす歯科補綴の治療イノベーション

    加来賢, 石本卓也, 佐々木宗輝

    日本補綴歯科学会誌(Web)   8   103 (WEB ONLY)   2016.7

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  • 卵巣除去ラットに対する皮質骨量の増加による大腿骨の破壊強度の増大(Fracture Strength of Femoral Bone Increased by Gain of Cortical Bone Volume on Ovariectmized Rats)

    ロサレス・ロカバド・ホアン・マルセロ, 加来 賢, 野崎 浩佑, 井田 貴子, 魚島 勝美

    日本補綴歯科学会誌   8 ( 特別号 )   262 - 262   2016.7

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  • 骨質研究がもたらす歯科補綴の治療イノベーション 骨質としてのコラーゲン翻訳後修飾と架橋形成

    加来 賢

    日本補綴歯科学会誌   8 ( 特別号 )   103 - 103   2016.7

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  • SDF-1/CXCR4による歯根膜への骨髄由来細胞の誘導

    加来賢, 北見恩美, ROCAVADO JM Rosales, 井出貴子, 秋葉陽介, 魚島勝美

    日本補綴歯科学会誌(Web)   8   2016

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  • 骨強度に重要なコラーゲン架橋の変化は骨代謝に影響を及ぼす

    井田 貴子, 加来 賢, 北見 恩美, ロサレス・ロカバド・ホアン・マルセロ, 魚島 勝美

    新潟歯学会雑誌   45 ( 1 )   25 - 26   2015.6

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  • コラーゲン・クロスリンクの変化は骨代謝に影響を及ぼす

    井田 貴子, 加来 賢, 北見 恩美, Rosales Rocabado Juan Marcelo, 魚島 勝美

    日本結合組織学会学術大会プログラム・抄録集   47回   49 - 49   2015.5

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  • 硬軟組織の難治性疾患に対する病因解明と治療方法開発に向けての取り組み

    熱田 生, 松浦 尚志, 加来 賢

    日本補綴歯科学会誌   7 ( 特別号 )   108 - 108   2015.5

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  • コラーゲン架橋の変化は骨強度のみならず骨代謝に影響を及ぼす

    井田 貴子, 加来 賢, 北見 恩美, Rosales Rocabado Juan Marcelo, 魚島 勝美

    日本補綴歯科学会誌   7 ( 特別号 )   237 - 237   2015.5

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  • 矯正的歯の移動における歯根膜中のコラーゲン修飾酵素の発現

    北見 公平, 加来 賢, 魚島 勝美, 齋藤 功

    日本矯正歯科学会大会プログラム・抄録集   73回   197 - 197   2014.10

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  • コラーゲン・クロスリンクの変化が骨芽細胞、破骨細胞に及ぼす影響

    井田 貴子, 加来 賢, 北見 恩美, 魚島 勝美

    Journal of Oral Biosciences Supplement   2014   141 - 141   2014.9

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  • 多機能性エピジェネティクス化合物による骨増成法への多面的アプローチ

    秋葉 陽介, 江口 香里, 秋葉 奈美, 北見 恩美, マルセロ・ロサレス, 加来 賢, 魚島 勝美

    日本補綴歯科学会誌   6 ( 特別号 )   264 - 264   2014.5

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  • 歯根膜には大腿骨骨髄に由来する幹細胞が存在する

    加来 賢, 北見 恩美, Rosales Rocabado Juan Marcelo, 井田 貴子, 秋葉 陽介, 魚島 勝美

    日本補綴歯科学会誌   6 ( 特別号 )   104 - 104   2014.5

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  • コラーゲン・クロスリンクが骨芽細胞分化、破骨細胞分化に及ぼす影響

    井田 貴子, 加来 賢, 北見 恩美, Rosales Rocabado Juan, Marcelo Rosales, 加来 咲子, 魚島 勝美

    日本補綴歯科学会誌   6 ( 特別号 )   259 - 259   2014.5

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  • コラーゲン・クロスリンクが骨芽細胞に及ぼす影響

    井田 貴子, 加来 賢, 北見 恩美, ロサレス・マルセロ, 魚島 勝美

    日本補綴歯科学会誌   6 ( 2 )   E65 - E65   2014.4

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  • 幹細胞を用いた組織再生法の新機軸-内在性幹細胞の動員-

    加来賢, 秋山謙太郎, 秋葉陽介, 秋田大輔

    日本補綴歯科学会学術大会プログラム・抄録集   123rd   2014

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  • HDACi処理細胞移植による骨増成法の検討

    江口 香里, 秋葉 陽介, 秋葉 奈美, 北見 恩美, 加来 賢, 魚島 勝美

    Journal of Oral Biosciences Supplement   2013   189 - 189   2013.9

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  • 移植細胞の初期動態とストレスタンパク質HSP27導入による影響

    北見 恩美, 加来 賢, 井田 貴子, 秋葉 陽介, 魚島 勝美

    Journal of Oral Biosciences Supplement   2013   155 - 155   2013.9

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  • 歯根膜における骨髄由来細胞の局在と幹細胞マーカーの発現

    加来 賢, 北見 恩美, 井田 貴子, 秋葉 陽介, 魚島 勝美

    Journal of Oral Biosciences Supplement   2013   134 - 134   2013.9

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  • 歯根膜におけるプライマリー・シリアの出現率と過剰咬合による変化

    井田 貴子, 加来 賢, 北見 恩美, 魚島 勝美

    Journal of Oral Biosciences Supplement   2013   142 - 142   2013.9

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  • 移植細胞の初期動態とHSP27過剰発現骨芽細胞に関する分析

    北見 恩美, 加来 賢, 井田 貴子, 秋葉 陽介, 魚島 勝美

    新潟歯学会雑誌   43 ( 1 )   73 - 74   2013.6

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  • ラットの骨治癒に及ぼすバルプロ酸(VPA)の効果(Effect of Valproic Acid (VPA) on Bone Healing in Rat)

    Rashid Md. Mamunur, Akiba Yosuke, Kaku Masaru, Akiba Nami, Uoshima Katsumi

    新潟歯学会雑誌   43 ( 1 )   74 - 74   2013.6

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  • 歯根膜における骨髄由来細胞の局在と幹細胞マーカーの発現

    加来 賢, 野澤 恩美, Rosales Juan Marcelo, 井田 貴子, 秋葉 陽介, 魚島 勝美

    日本補綴歯科学会誌   5 ( 特別号 )   222 - 222   2013.5

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  • 各種HDACiが骨分化能、骨形成能に与える影響に関する研究

    江口 香里, 秋葉 奈美, Rosales Juan Marcelo, 野澤 恩美, 加来 賢, 秋葉 陽介, 魚島 勝美

    日本補綴歯科学会誌   5 ( 特別号 )   275 - 275   2013.5

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  • ジルコニア製インプラントドリルの生物学的評価に関する研究

    秋葉 陽介, 江口 香里, 秋葉 奈美, Rosales Juan Marcelo, 野澤 恩美, 加来 賢, 魚島 勝美

    日本補綴歯科学会誌   5 ( 特別号 )   167 - 167   2013.5

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  • 移植細胞の初期動態とHSP27の導入による細胞移植法の検討

    野澤 恩美, 加来 賢, Rosales Juan Marcelo, 井田 貴子, 秋葉 陽介, 魚島 勝美

    日本補綴歯科学会誌   5 ( 特別号 )   248 - 248   2013.5

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  • 歯根膜の部位によるプライマリー・シリア出現率の違いと過剰咬合による変化

    井田 貴子, 加来 賢, 野澤 恩美, Rosales Juan Marcelo, 加来 咲子, 魚島 勝美

    日本補綴歯科学会誌   5 ( 特別号 )   209 - 209   2013.5

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  • Early Behaviors of Transplanted Cells and the Effect of HSP27 on the Cell Survival

    Megumi Kitami, Masaru Kaku, Takako Ida, J. M. Rosales, Kastumi Uoshima

    JOURNAL OF BONE AND MINERAL RESEARCH   28   2013.2

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  • ラットにおけるHDACI全身投与による骨形成促進作用の検討

    秋葉 陽介, ラシッド・マムヌール, 秋葉 奈美, 野澤 恩美, 加来 賢, 魚島 勝美

    日本補綴歯科学会誌   5 ( 1 )   E128 - E128   2013.1

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  • ローヤルゼリー摂取による卵巣摘出ラットの骨量、骨質への影響

    加来 賢, Rosales Rocavado J.M., 野澤 恩美, 魚島 勝美

    日本補綴歯科学会誌   5 ( 1 )   E127 - E127   2013.1

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  • 移植細胞の初期動態とストレスタンパク質HSP27導入による影響

    北見恩美, 北見恩美, 加来賢, 井田貴子, 秋葉陽介, 秋葉陽介, 魚島勝美, 魚島勝美

    Journal of Oral Biosciences Supplement (Web)   2013   2013

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  • 歯根膜における骨髄由来細胞の局在と幹細胞マーカーの発現

    加来賢, 北見恩美, 井田貴子, 秋葉陽介, 秋葉陽介, 魚島勝美, 魚島勝美

    Journal of Oral Biosciences Supplement (Web)   2013   2013

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  • HDACi処理細胞移植による骨増成法の検討

    江口香里, 秋葉陽介, 秋葉陽介, 秋葉奈美, 秋葉奈美, 北見恩美, 北見恩美, 加来賢, 魚島勝美, 魚島勝美

    Journal of Oral Biosciences Supplement (Web)   2013   2013

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  • 荷重負担下ラットインプラント周囲骨の骨応力分布と骨組織変化の関連性

    高野 遼平, 長澤 麻沙子, 野澤 恩美, Rosales Rocabado Juan Marcelo, 加来 賢, Stegaroiu Roxana, 魚島 勝美

    新潟歯学会雑誌   42 ( 2 )   135 - 135   2012.12

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  • 機械的刺激に誘導されるコラーゲン修飾酵素が歯根膜組織に及ぼす影響

    加来 賢, 野澤 恩美, 秋葉 陽介, 魚島 勝美

    Journal of Oral Biosciences Supplement   2012   156 - 156   2012.9

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  • ヒストン脱アセチル化阻害剤全身投与による骨形成促進作用の検討

    秋葉 陽介, 野澤 恩美, 加来 賢, 魚島 勝美

    Journal of Oral Biosciences Supplement   2012   164 - 164   2012.9

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  • HDACIによる骨代謝制御機構を応用した骨造成法

    秋葉 陽介, 加来 賢, 魚島 勝美

    日本歯科医師会雑誌   65 ( 5 )   620 - 620   2012.8

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  • 機械的刺激によるコラーゲン翻訳後修飾を介した歯根膜組織安定化機構

    加来 賢, 野澤 恩美, Rosales J.M. Marcelo, 秋葉 陽介, 魚島 勝美

    日本歯科医師会雑誌   65 ( 5 )   617 - 617   2012.8

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  • 一口腔単位での問題発見・解決能力の涵養を目的とした治療方針立案演習

    秋葉 陽介, 加来 賢, 秋葉 奈美, 長澤 麻沙子, 魚島 勝美

    日本歯科医学教育学会総会・学術大会プログラム・抄録集   31回   68 - 68   2012.7

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  • ラットにおけるHDACI全身投与による骨再生

    秋葉 陽介, ラシッド・マムヌール, 秋葉 奈美, 加来 賢, 魚島 勝美

    日本補綴歯科学会誌   4 ( 特別号 )   198 - 198   2012.5

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  • ローヤルゼリーはコラーゲン翻訳後修飾およびクロスリクを介して骨質の改善に寄与する

    Rosales-Rocabado J.M., 加来 賢, 野澤 恩美, 魚島 勝美

    日本補綴歯科学会誌   4 ( 特別号 )   200 - 200   2012.5

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  • メカニカルストレスに対する生体応答 補綴学的意義と展望 メカニカルストレスと歯根膜の恒常性維持

    加来 賢

    日本補綴歯科学会誌   4 ( 特別号 )   41 - 41   2012.5

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  • Heat Shock Protin27の過剰発現が骨芽細胞の抗アポトーシス能・分化能に及ぼす影響

    野澤 恩美, 加来 賢, Rosales-Rocabado J.M., 魚島 勝美

    日本補綴歯科学会誌   4 ( 特別号 )   142 - 142   2012.5

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  • ヒストン脱アセチル化阻害剤全身投与による骨形成促進作用の検討

    秋葉陽介, 野澤恩美, 加来賢, 魚島勝美

    Journal of Oral Biosciences Supplement (Web)   2012   2012

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  • 機械的刺激に誘導されるコラーゲン修飾酵素が歯根膜組織に及ぼす影響

    加来賢, 野澤恩美, 秋葉陽介, 魚島勝美, 魚島勝美

    Journal of Oral Biosciences Supplement (Web)   2012   2012

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  • In vitroにおける骨膜由来細胞の造骨細胞分化と石灰化能(Osteoblastic Differentiation and Mineralization Ability of Periosteum Derived Cells in vitro)

    Rocabado Juan Marcelo Rosales, 加来 賢, 野沢 恩美, 秋葉 陽介, 魚島 勝美

    新潟歯学会雑誌   41 ( 2 )   129 - 129   2011.12

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  • ヒストン脱アセチル化酵素阻害薬は骨膜由来細胞に骨原性細胞分化能を与える(Histone Deacetylase inhibitors have osteogenic differentiation effect on periosteum derived cells)

    Bhuiyan Al-Amin Md., 秋葉 陽介, 加来 賢, Rocabado Juan Marcelo Rosales, 魚島 勝美

    新潟歯学会雑誌   41 ( 2 )   129 - 129   2011.12

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  • 骨膜・歯根膜細胞に対してヒストン脱アセチル化酵素阻害薬は細胞分化を活性化し、骨形成を促進する効果がある(Histone Deacetylase inhibitors have osteogenic differentiation effect on periosteum and periodontal ligament cells)

    Bhuiyan Al-Amin, Akiba Yosuke, Kaku Masaru, Takano Ryoheii, Uoshima Katsum

    日本口腔インプラント学会誌   24 ( 特別号 )   165 - 165   2011.8

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  • ラット骨再生に対してヒストン脱アセチル化酵素阻害薬が与える影響(Effect of Histone Deacetylase Inhibitor on Bone Regeneration in Rat)

    Mamunur Rashid M.D., Akiba Yosuke, Kaku Masaru, Nagasawa Masako, Uoshima Katsumi

    日本補綴歯科学会誌   3 ( 特別号 )   144 - 144   2011.5

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  • 歯根膜におけるXII型コラーゲンの局在と石灰化能への影響

    加来 賢, Rosales Rocavado J.M., 野澤 恩美, 魚島 勝美

    日本補綴歯科学会誌   3 ( 特別号 )   308 - 308   2011.5

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  • 骨芽細胞の分化過程におけるsmall Heat Shock Proteinの発現

    野澤 恩美, 加来 賢, Rosales Rocavado J.M., 魚島 勝美

    日本補綴歯科学会誌   3 ( 特別号 )   313 - 313   2011.5

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  • 歯根膜をモデルとしたメカノバイオロジー研究の推進 コラーゲン・クロスリンクを介した組織安定化機構

    加来 賢, 魚島 勝美

    日本歯科医学会誌   30   82 - 82   2011.3

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  • Stem Cell Capability of Neural Crest Derived Cells in Adult PDL

    KAKU M, KOMATSU Y, MISHINA Y, UOSHIMA K, KO Ching-Chang

    1 ( 118 )   80 - 80   2010.8

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  • 機械的刺激は骨芽細胞においてコラーゲン修飾/分解酵素の発現を特異的に制御する

    加来 賢, 山内 三男, 魚島 勝美

    日本補綴歯科学会誌   2 ( 2 )   E64 - E64   2010.4

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  • 間葉系幹細胞の骨形成系細胞誘導におけるヒストン脱アセチル化阻害剤の影響について

    秋葉陽介, アルアミン ブイアン, 川崎真依子, 加来賢, 加来賢, 魚島勝美, 魚島勝美

    日本補綴歯科学会学術大会プログラム・抄録集   119th   2010

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  • 咀嚼能率検査とQOLアンケートによる固定性インプラント義歯と部分床義歯の比較

    川崎真依子, 吉田恵子, 加来賢, 秋葉陽介, 長澤麻沙子, 藤井規孝, ROSALES M J.M., AL-AMIN MD.B, RASHID MD.M, 魚島勝美

    日本補綴歯科学会学術大会プログラム・抄録集   119th   2010

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  • コラーゲン修飾酵素の特異的発現が機械的刺激による歯根膜組織の安定化を制御する

    加来賢, 加来賢, 川崎真依子, 秋葉陽介, ROSALES M, RASHID M, 魚島勝美, 魚島勝美

    日本補綴歯科学会学術大会プログラム・抄録集   119th   2010

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  • インプラント咬合動物実験モデルにおける骨の組織学的観察

    長澤麻沙子, 加来賢, 秋葉陽介, 吉田恵子, 川崎真依子, ROSALES Marcelo, AL‐AMIN Buiyan, 魚島勝美, 前田健康

    J Oral Biosci   51 ( Suppl. )   101 - 101   2009.8

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    J-GLOBAL

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  • 繰返し荷重時の歯周組織応答を検討する実験モデル確立の試み

    野崎 浩佑, 加来 賢, 渡辺 希江, 木津喜 裕子, 公田 有子, 山下 靖雄, 三浦 宏之

    日本補綴歯科学会雑誌   51 ( 116回特別 )   102 - 102   2007.5

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  • 歯科用セラミックスの繰返し疲労と静疲労

    高橋 英和, 金 景月, 加来 賢, 中里 孝宏, 北崎 祐之, 岩崎 直彦, 青柳 裕仁, 三浦 宏之, 黒崎 紀正

    歯界展望   特別号 ( 健康な心と身体は口腔から-発ヨコハマ2004- )   316 - 316   2005.6

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  • 歯科用セラミックスの繰返し疲労と静疲労

    高橋 英和, 金 景月, 加来 賢, 中里 孝宏, 北崎 祐之, 岩崎 直彦, 青柳 裕仁, 三浦 宏之, 黒崎 紀正

    日本歯科医師会雑誌   57 ( 4 )   422 - 422   2004.7

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  • Excessive occlusal load induces apoptosis among periodontal ligament.

    M. Kaku, K. Uoshima, Y. Yamashita, H. Miura

    JOURNAL OF DENTAL RESEARCH   82   B287 - B287   2003.6

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    Web of Science

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  • キャスタブルセラミックスにおける静疲労及び繰返し疲労の影響について

    加来 賢, 高橋 英和, 北崎 祐之, 三浦 宏之

    日本補綴歯科学会雑誌   46 ( 108回特別 )   155 - 155   2002.10

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  • Establishment of the Rat Experiment Model to Reference the Mechanism of Occlusal Trauma Pathogenesis

    KAKU M, UOSHIMA K, YAMASHITA Y, MIURA H

    46 ( 107 )   83 - 83   2002.5

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  • 生理的歯牙変位様相と環境因子との関連について

    アマルサイハン・バザル, 益田 高行, 岡田 大蔵, 石原 弘文, 新木 敏之, 菅野 多真, 徳田 彩子, 加来 賢, 三浦 宏之

    日本補綴歯科学会雑誌   45 ( 2 )   377 - 377   2001.4

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Presentations

  • メカニカルストレスの制御を基盤とした歯科補綴治療戦略 先端テクノロジーが拓く2040年の補綴治療 〜バックキャスティング思考によるイノベーション戦略〜 Invited

    第24回日本歯科医学会学術大会  2021.9 

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  • 間葉系幹細胞による歯根膜維持メカニズムの解明と展望 〜間葉系幹細胞の研究から考える生物科学としての補綴歯科治療〜 Invited

    第130回日本補綴歯科学会学術大会  2021.6 

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  • 骨質をコラーゲンの生合成から理解する. 海外の基礎研究はインプラント治療をどう変えたか？ Invited

    第48回公益社団法人日本口腔インプラント学会学術大会  2018 

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  • コラーゲンと細胞の多様性から見た歯根膜 Invited

    BioForum@Dental School 岡山大学  2017 

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  • Mechano-regulations of Collagen Biosynthesis and Related Cell Behaviors in Periodontal Ligament. Invited

    International Symposium on Development of Human Resources in Practical Oral Health and Treatment.  2016 

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  • 骨質研究がもたらす歯科補綴の治療イノベーション Invited

    第125回日本補綴歯科学会学術大会  2016 

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  • 硬軟組織難治性疾患に対する組織再建と研究戦略 Invited

    第124回日本補綴歯科学会学術大会  2015 

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  • コラーゲン生合成における翻訳語修飾とその分子機構 Invited

    第11回北里大学農医連携シンポジウム  2015 

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  • Mechanoregulations of Collagen Biosynthesis in Periodontal Ligament, Invited

    International Symposium on Mechanobiology 2014  2014 

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  • 歯根膜には大腿骨骨髄に由来する幹細胞が存在する Invited

    第123回日本補綴歯科学会  2014 

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  • 幹細胞を用いた組織再生法の新機軸−内在性幹細胞の動員− Invited

    第123回日本補綴歯科学会  2014 

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  • Mechanical Stress Mediated Tissue Homeostasis in Periodontal Ligament, Invited

    International Symposium on Human Resource Development towards Global Initiative  2013 

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  • メカニカルストレスと歯根膜の恒常性維持 Invited

    第121回日本補綴歯科学会  2012 

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  • 機械的刺激による骨組織の安定化機構―コラーゲン修飾酵素によるクロスリンクの生成― Invited

    第27回 歯科医学を中心とした総合的な研究を推進する集い  2011 

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Awards

  • 優秀発表賞（若手セミナー）

    2025.6   日本結合組織学会  

    小林水輝,加来賢,他

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  • 優秀論文賞

    2025.5   日本補綴歯科学会  

    小野喜樹,加来賢,他

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  • 課題講演賞

    2025.5   日本補綴歯科学会  

    小野喜樹,加来賢,他

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  • 優秀発表賞（若手セミナー）

    2024.6   日本結合組織学会  

    小野喜樹,加来賢,他

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  • 優秀演題賞

    2024.3   日本再生医療学会  

    小野喜樹, 加来 賢,他

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  • 優秀演題賞

    2024.3   日本再生医療学会  

    小林水輝, 加来 賢,他

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  • Young Investigator Award

    2023.6   日本結合組織学会  

    土橋 梓, 加来 賢,他

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  • デンツプライ賞

    2023.5   日本補綴歯科学会  

    土橋 梓, 加来 賢,他

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  • 新潟歯学会奨励賞

    2023.3   新潟歯学会  

    新井萌生, 加来 賢

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  • 学術奨励賞

    2022.10   日本矯正歯科学会  

    水越 優, 加来 賢,他

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  • 優秀賞

    2022.8   先端歯学スクール  

    小野喜樹, 加来 賢,他

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  • 課題口演賞

    2022.7   日本補綴歯科学会  

    土橋 梓, 加来 賢,他

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  • Young Investigator Award

    2022.6   日本結合組織学会  

    土橋 梓, 加来 賢,他

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  • Paper Award

    2018  

    Ida T, Kaku M, Kitami M, Terajima M, Rosales Rocabado JM, Akiba Y, Nagasawa M, Yamauchi M, Uoshima K

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  • 新潟歯学会賞

    2017.3   新潟歯学会  

    北見公平, 加来 賢

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  • Best Oral Presentation Award

    2014.7   Asian Academy of Osseointegration  

    Ida T, Kaku M, et al.

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  • 課題口演優秀賞

    2014.5   日本補綴歯科学会  

    加来 賢

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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  • Best Oral Presentation Award

    2013.4   CPS-JPS-KAP  

    Kaku M

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    Award type：Award from international society, conference, symposium, etc.  Country：Japan

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  • Poster Presentation Award 1st Place

    2012.12   Asian Academy of Prosthodontics  

    Nozawa M, Kaku M, et al.

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  • Poster Presentation Award 1st Place

    2011.10   Asian Academy of Prosthodontics  

    Nozawa M, Kaku M, et al.

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  • デンツプライ賞

    2002.10   日本補綴歯科学会  

    加来 賢

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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Research Projects

  • Extracellular matrix controlled stem cell differentiation and its application toward the regeneration of periodontal ligament

    Grant number：24K02630

    2024.4 - 2027.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\18590000 （ Direct Cost: \14300000 、 Indirect Cost：\4290000 ）

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  • 加齢マウス歯根膜組織の深層プロテオーム解析：組織応答を担う細胞外環境の変化

    Grant number：23K09412

    2023.4 - 2026.3

    System name：科学研究費助成事業

    Research category：基盤研究(C)

    Awarding organization：日本学術振興会

    北見 公平, 加来 賢, 齋藤 功

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

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  • 骨芽細胞のⅠ型コラーゲンと基質小胞の分泌経路におけるRabタンパク質の機能解明

    Grant number：23K09117

    2023.4 - 2026.3

    System name：科学研究費助成事業

    Research category：基盤研究(C)

    Awarding organization：日本学術振興会

    柿原 嘉人, 加来 賢, 三上 剛和

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

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  • Development of predictive autotransplantation based on the mechanism of periodontal ligament homeostasis

    Grant number：23K09293

    2023.4 - 2026.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\4810000 （ Direct Cost: \3700000 、 Indirect Cost：\1110000 ）

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  • Elucidation of stem cell differentiation mechanism by genetic cell tracing and gene network analysis

    Grant number：21K19895

    2021.7 - 2024.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Research category：Grant-in-Aid for Challenging Research (Exploratory)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\6240000 （ Direct Cost: \4800000 、 Indirect Cost：\1440000 ）

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  • Proteome analysis of periodontal ligament matrix and development of regenerative material

    Grant number：21H03127

    2021.4 - 2024.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\17420000 （ Direct Cost: \13400000 、 Indirect Cost：\4020000 ）

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  • Trans-omics analysis of the difference between Cortical and Trabecular bone.

    Grant number：21K09998

    2021.4 - 2024.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

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  • 移植骨の骨細胞ネットワーク再構築と骨質に着目した自家骨移植の至適条件探索

    Grant number：20H03876

    2020.4 - 2024.3

    System name：科学研究費助成事業 基盤研究(B)

    Research category：基盤研究(B)

    Awarding organization：日本学術振興会

    魚島 勝美, 加来 賢, 長澤 麻沙子, 秋葉 陽介

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    Grant amount：\15600000 （ Direct Cost: \12000000 、 Indirect Cost：\3600000 ）

    インプラント適用や補綴装置適用の際に当該部位の骨が不足する場合、比較的簡便であること、コストが低いことなどの理由で、臨床的には口腔内からの最小限の骨採取で骨増生に一定の効果がある自家骨移植が現在広く行われている。本研究の目的は、従来まったく着目されていない、移植骨の骨細胞ネットワークの状態と移植骨の生着および長期予後との関係を見出すことである。そのためには、実験動物を用いて適切な実験モデルを構築することが重要で、先ず初年度はラットを用いた実験モデルを構築した。その後ラットで行った方法がマウスでも同様に効果的であるか否かを検証する必要があったが、COVID19による影響で、実験が中断した。そこで、今年度はマウスを用いて同様のモデルを構築するために、ラットと同じ手法で実験を行ったが個体の大きさが異なるために難渋し、年度の広範囲なって初めて実験モデルを構築することに成功した。その後、実験期間を1週、2週、4週として脱灰薄切標本を作製し、移植骨と母骨が良好に結合し、両者間の間隙に良好な骨形成が得られていることを確認した。さらに、骨形成ネットワークの再生を観察するために、採取した組織に透明化処理（CUBIC法）を施し、骨細胞ネットワークをPhalloidinにて染色した後に、二光子顕微鏡により移植骨を取り巻く骨細胞ネットワークの3次元的解析を行うための準備をした。また、実験動物に対して標本採取の直前にSulforhodamine101(8mM)を投与して血漿成分を蛍光標識し、二光子顕微鏡（本学脳研究所に設置）によって毛細血管の再生を立体的に可視化する試みも行っている。

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  • Investigation of the mechanism of ortho- and non-keratinization pattern using different stiffness of scaffold and its application for fabrication of tissue-engineered oral mucosa

    Grant number：20H03870

    2020.4 - 2023.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    Izumi Kenji

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    Grant amount：\17680000 （ Direct Cost: \13600000 、 Indirect Cost：\4080000 ）

    Although histology of oral mucosa often demonstrates dramatic change of keratinization pattern, for instance, at muco-gingival fold. However, the mechanism is not well-elucidated. This study aimed to investigate how the histological differences are regulated in terms of mechanobiology. Using human primary oral keratinocytes culturing on two types of collagen matrices with different stiffness and regular culture dishes, we examined micro-array analyses, histological observation of tissue-engineered oral mucosa and measurement of Young’s modulus. The gene expression analysis revealed that there are many genes that are up- and down-regulated depending on the stiffness. However, there are few genes specific to biological functions of oral keratinocytes. Furthermore, there were scarce differences of histological appearances and Young's modulus among cells grown on matirces with different stiffness. It may be required to increase the number of samples and matrix stiffness to be examined.

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  • in vivo analysis of the occlusal force-responsive tissue stem cells in periodontal ligament

    Grant number：19K10200

    2019.4 - 2021.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

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  • Development of a novel cellulose scaffold to potentiate the transplanted cells survival for bone regeneration

    Grant number：18K09680

    2018.4 - 2021.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    Rosales Marcelo

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

    In this investigation, we used Methylcellulose (MC) to develop and optimize a novel scaffold material for bone cell transplantation. A series of MC scaffolds with different porosity, cross-link density, and size were fabricated. Results showed that Sodium Chloride (NaCl) has a great effect on the homogeneity and porosity of the scaffold in a dose-dependent manner. Crosslinking using carbonyldiimidazole (CDI) at different concentrations showed no significant changes in the scaffold’s characteristics. As a result of the lyophilization procedure, the thickness of the scaffold was significantly reduced; consequently, affecting the scaffold's structure and compromising the mechanical strength needed for tissue transplantation. Although the production of MC scaffolds was achieved, homogeneity between samples was rather difficult to obtain. Thus, further optimization is required for the production of viable cell transplantation scaffolds using methylcellulose.

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  • Elucidation of fate path and regulatory factors of periodontal ligament tissue stem cells

    Grant number：18H02989

    2018.4 - 2021.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    Masaru Kaku

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\17420000 （ Direct Cost: \13400000 、 Indirect Cost：\4020000 ）

    The periodontal ligament (PDL) plays a vital role in oral function and has a multi-layered structure in which cell groups with contradictory characteristics of calcification / non-calcification are stacked. However, the details of the cells constituting each layer are still unclear. An accurate and integrated understanding of the PDL’s multilayer structure, especially the reproduction of the non-mineralized region characteristic of the PDL, will be fundamental for enabling PDL regeneration in natural teeth and the development of the dental implant with PDL. In this study, we used cell proliferation / metabolic activity as an index to clarify the fate and differentiation of PDL tissue stem cells and some of the regulatory mechanisms.

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  • Analysis of the molecular mechanism of the mechanical stress response in osteoblasts through primary cilia

    Grant number：17K11639

    2017.4 - 2020.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    Kakihara Yoshito

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    Grant type：Competitive

    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

    In this study, we investigated how osteoblasts, which are involved in bone formation, perceive and respond to external stress. Molecular chaperones are known to be involved in a variety of stress responses. We focused our research on Pontin-Reptin chaperone, which is one of the emerging molecular chaperone. Our results suggest that the Pontin-Reptin mediates between cell cycle and primary cilia formation in osteoblasts.

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  • Analysis of the new role of a collagen network for the morphogenesis during the bone modeling

    Grant number：16H05549

    2016.4 - 2019.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    KAMIOKA HIROSHI, ADACHI Taiji, JAGER Edwin

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    Grant type：Competitive

    Grant amount：\17160000 （ Direct Cost: \13200000 、 Indirect Cost：\3960000 ）

    We performed three-dimensional construction of collagen fibers in the osteogenic phase by using FIB-SEM. Furthermore, since this analysis extends to a cubic region of 25 micrometers per side, it was possible to capture a cellular network containing multiple osteocytes simultaneously. From these observations, processes extending from the osteoblasts to the matrix side have a certain regularity so as to avoid bundled collagen fibers. Furthermore, the bones pretreated with BAPN, which inhibits collagen fiber aggregation, cell processes extending from osteoblasts have no specific orientation. Thus, it has been revealed that changing the nature of bone matrix affects osteocyte network formation.

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  • Investigation of Bone Marrow-derived Stem Cells in Periodontal Ligament(Fostering Joint International Research)

    Grant number：15KK0337

    2016 - 2019

    System name：Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    Research category：Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    Awarding organization：Japan Society for the Promotion of Science

    Kaku Masaru

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    Grant amount：\14430000 （ Direct Cost: \11100000 、 Indirect Cost：\3330000 ）

    Periodontal ligament plays important roles in the occlusal function therefore, its maintenance/regeneration is essential for achieving healthy longevity of life. It has been speculated that the growth factors and extracellular matrix proteins secreted by periodontal ligament-constituting cells regulate the maintenance/differentiation of periodontal ligament in a site-specific manner. However, the site-specificity of the periodontal ligament-constituting cells are still not well characterized. The present study revealed that not only the cells existing from the developmental stage but also the hematogenously supplied bone marrow-derived cells are present in the periodontal ligament. These cells contribute to the maintenance of periodontal ligament homeostasis.

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  • Development of optimally cross-linked collagen scaffold directed to differentiation control of stem cells

    Grant number：15K15704

    2015.4 - 2019.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Research category：Grant-in-Aid for Challenging Exploratory Research

    Awarding organization：Japan Society for the Promotion of Science

    Kaku Masaru

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    Grant amount：\3640000 （ Direct Cost: \2800000 、 Indirect Cost：\840000 ）

    This study is based on the idea that control of collagen cross-linking in collagen-based cell transplantation carrier not only changes the mechanical properties of the material but also can promote osteoblast differentiation of mesenchymal stem cells. The aim of this project was to analyze the effect of collagen crosslinking on osteoblast differentiation of mesenchymal stem cells. The addition of a crosslinking inhibitor in the osteoblast culture system under certain conditions has succeeded in selectively reducing collagen crosslinking without affecting the amount of collagen produced. Furthermore, it was revealed that changes in collagen crosslinking affect proliferation, initial adhesion, and osteoblast differentiation of mesenchymal stem cells.

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  • Investigation of Bone Marrow-derived Stem Cells in Periodontal Ligament

    Grant number：26293407

    2014.4 - 2018.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    KAKU Masaru

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\16640000 （ Direct Cost: \12800000 、 Indirect Cost：\3840000 ）

    The periodontal ligament (PDL) plays important roles in tooth function. The PDL harbors a remarkable reserve of multipotent stem cells, which maintain various types of cells. However, the sources of these stem cells, other than their developmental origin, are not well understood. In the present study, to elucidate the recruitment of bone marrow (BM)-derived stem cells in the PDL, green fluorescent protein (GFP)-expressing BM-derived cells were transplanted into the femoral BM of immunodeficient rats. Four weeks after cell transplantation, GFP-positive cells were detected in the PDL. To evaluate the functional significance of these BM-derived cells to the PDL, tooth replantation was performed. Seven days after tooth replantation, the number of GFP-positive cells significantly increased in PDL. These results indicate that the stem cells and their progeny in PDL are not only derived from their developmental origin but are also supplied from the BM via the blood as the need arises.

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  • Research on the Effect of Bone Quaility (Collagen) on Bone Metabolism and Its Mechanism

    Grant number：26293408

    2014.4 - 2018.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    Uoshima Katsumi, MUBARAK Suliman

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    Grant type：Competitive

    Grant amount：\15860000 （ Direct Cost: \12200000 、 Indirect Cost：\3660000 ）

    An in Vitro experiment using cultured cells was first conducted in order to analyze the influence of bone tissue collagen crosslink formation failure on osteoblast / osteoclast activity. As a result, in the cell culture system, the decrease in the amount of crosslink in the matrix increased the activity of osteoblasts and decreased the activity of osteoclasts. Serum bone formation markers increased in vivo and bone resorption markers tended to decrease. Furthermore, bone grafting experiments and implant installation experiments using an animal model of cross link formation failure resulted in bone formation around transplanted bone faster than control. A large amount of immature bone was formed around the implants earlier than the control.

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  • biological evaluation of curcumin contributions for the bone formation

    Grant number：25462988

    2013.4 - 2017.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    Akiba Nami

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    Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）

    Osteoporosis is one of the risk factor for implant therapy. Curcumin is known as a functional healthy food and able to remove active oxygen spices. Curcumin thought to be contributed to help bone formation and save people from osteoporosis. The aim of this study is to elucidate the mechanisms of acceleration for bone formation by curcumin. We fed curcumin to the normal rat and OVX rat, then observed maxillary cylindrical cavity healing, calvarias defect and femoral defect. Among these all samples, there are no significant difference could be seen.

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  • Mechanical Stress Regulates Collagen Crosslinking in Periodontal Ligament

    Grant number：24792068

    2012.4 - 2014.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Research category：Grant-in-Aid for Young Scientists (B)

    Awarding organization：Japan Society for the Promotion of Science

    KAKU Masaru

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

    The purpose of this study was to investigate the effects of excessive mechanical loading on the expression of collagen-modifying enzymes and subsequent crosslinking in PDL. Upon mechanical loading, gene expression of LH2, a collagen telopeptide specific Lysyl hydroxylase, was significantly upregulated, while that of COL1A2, encoding type I collagen was not affected. The expression of LH2 in PDL cells was also confirmed on an excessive occlusal loading rat model and expression was limited on the alveolar bone side of PDL in vivo. These results indicate that mechanical loading induces expression of collagen-modifying enzyme and controls subsequent collagen cross-linking, which are important for achieving long-term tissue maintenance and stability of PDL.

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  • Development of Cementum/PDL complex using human neural crest derived stem cells from PDL.

    Grant number：22791875

    2010 - 2011

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Research category：Grant-in-Aid for Young Scientists (B)

    Awarding organization：Japan Society for the Promotion of Science

    KAKU Masaru

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    Grant amount：\3900000 （ Direct Cost: \3000000 、 Indirect Cost：\900000 ）

    The purpose of this study was to characterize the neural crest(NC) derived cells in adult periodontal ligament by using NC-traceable transgenic mice. Our study reveled that the distribution and prevalence of NC derived cells in adult PDL. Furthermore, the distribution of NC stem cell marker and mesenchymal stem cell marker positive cells were distinct.

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  • 新規幹細胞源として期待される神経堤由来幹細胞の歯根膜からの分離解析

    Grant number：21890081

    2009 - 2010

    System name：科学研究費助成事業 研究活動スタート支援

    Research category：研究活動スタート支援

    Awarding organization：日本学術振興会

    加来 賢

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    Grant amount：\2600000 （ Direct Cost: \2000000 、 Indirect Cost：\600000 ）

    歯根膜は発生段階において間葉のみならず神経堤にも由来しているが、これまでに歯根膜において同定された幹細胞は間葉に由来するものばかりであった。そこで申請者らは神経堤由来細胞を追跡可能な遺伝子改変マウスを用いて免疫組織学的に検索を行ったところ、神経堤由来幹細胞の存在を強く示唆する知見を得る事に成功し、この研究では神経堤由来幹細胞を分離抽出し、その分化能および力学的刺激への応答能を解析し、歯周組織再建に向けた新たな幹細胞源を明らかにすることを目的とした。我々は組織標本上にて歯根膜細胞中のさらなる幹細胞マーカーの発現を検索し、HNK-1/CD57,SOX10の発現が、神経堤由来細胞において高頻度で見られることを明らかにした。現在マウスおよびヒト歯根膜由来細胞を用い、神経堤幹細胞について幹細胞能を維持した状態での培養の至適条件を検索中である。当初予定していた血清を含まない培養条件では生細胞が得られなかったため、神経幹細胞培養に用いられる添加物を使用した条件を検討中であるている。これまでのところ異なる培養条件において異なる形態を示す細胞群の分離に成功しており、さらなる条件の絞り込みをすすめている。また、使用を予定した遺伝子改変マウスはミシガン大学からの移管が大幅に遅れたため、東京医科歯科大学より移管する事とした。現在動物実験施設に於いて手続き中である。

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Teaching Experience (researchmap)

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Teaching Experience

  • 口腔インプラント学

    2024
    Institution name：新潟大学

  • 口腔インプラント治療学演習IA

    2024
    Institution name：新潟大学

  • 補綴歯科学臨床総合コースII

    2024
    Institution name：新潟大学

  • 口腔インプラント治療学演習IIA

    2024
    Institution name：新潟大学

  • 口腔インプラント治療学演習IIB

    2024
    Institution name：新潟大学

  • 口腔インプラント治療学演習IB

    2024
    Institution name：新潟大学

  • 補綴歯科学臨床総合コースI

    2024
    Institution name：新潟大学

  • デジタル技術を用いた補綴臨床治療学IA

    2024
    Institution name：新潟大学

  • 補綴歯科学基礎総合コースII

    2024
    Institution name：新潟大学

  • 補綴歯科学基礎総合コースI

    2024
    Institution name：新潟大学

  • デジタル技術を用いた補綴臨床治療学IIA

    2024
    Institution name：新潟大学

  • 生涯にわたる歯と咬合

    2022
    -
    2023
    Institution name：新潟大学

  • 総合模型実習

    2021
    Institution name：新潟大学

  • 固定性補綴治療学IB

    2021
    Institution name：新潟大学

  • 固定性補綴治療学IIB

    2021
    Institution name：新潟大学

  • 固定性補綴治療学IA

    2021
    Institution name：新潟大学

  • 固定性補綴治療学IIA

    2021
    Institution name：新潟大学

  • 歯の形態学

    2020
    Institution name：新潟大学

  • 固定性補綴治療学ⅠA

    2018
    Institution name：新潟大学

  • 欠損補綴学Ⅱ

    2014
    -
    2015
    Institution name：新潟大学

  • 歯冠修復学

    2011
    -
    2017
    Institution name：新潟大学

  • 早期臨床実習Ⅱ

    2011
    -
    2015
    Institution name：新潟大学

  • 選択実習Ⅱ

    2011
    -
    2013
    Institution name：新潟大学

  • 歯の形態と機能

    2010
    -
    2016
    Institution name：新潟大学

  • 生体材料学

    2010
    -
    2014
    Institution name：新潟大学

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