Updated on 2024/03/28

写真a

 
KAWASAKI Maiko
 
Organization
Academic Assembly Institute of Medicine and Dentistry SHIGAKU KEIRETU Associate Professor
Graduate School of Medical and Dental Sciences Oral Life Science Oral Biological Science Associate Professor
Title
Associate Professor
External link

Degree

  • 博士(歯学) ( 2010.3   新潟大学 )

Research Areas

  • Life Science / Oral biological science

  • Life Science / Surgical dentistry

  • Life Science / Developmental dentistry

Research History (researchmap)

  • Niigata University   Graduate School of Medical and Dental Sciences   Associate Professor

    2019.4

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  • King’s College London   Craniofacial Development and Stem Cell Biology   Research Fellow

    2011.1 - 2013.5

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  • Niigata University   Graduate School of Medical and Dental Sciences   Assistant Professor

    2010.4 - 2019.3

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Research History

  • Niigata University   Graduate School of Medical and Dental Sciences Oral Life Science Oral Biological Science   Associate Professor

    2019.4

  • Niigata University   Graduate School of Medical and Dental Sciences Oral Life Science Oral Biological Science   Assistant Professor

    2016.4 - 2019.3

  • Niigata University   Graduate School of Medical and Dental Sciences Oral Life Science   Assistant Professor

    2012.11 - 2016.3

  • Niigata University   University Medical and Dental Hospital Occlusal Function Fixed Prosthodontics   Assistant Professor

    2012.11 - 2016.3

  • Niigata University   University Medical and Dental Hospital Dental Health   Assistant Professor

    2010.4 - 2012.11

 

Papers

  • Ift88 regulates enamel formation via involving Shh signaling. Reviewed International journal

    Takehisa Kudo, Maiko Kawasaki, Katsushige Kawasaki, Fumiya Meguro, Jun Nihara, Izumi Honda, Madoka Kitamura, Akira Fujita, Kazuaki Osawa, Kaya Ichikawa, Takahiro Nagai, Yoko Ishida, Paul T Sharpe, Takeyasu Maeda, Isao Saito, Atsushi Ohazama

    Oral diseases   29 ( 4 )   1622 - 1631   2022.2

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    OBJECTIVES: The ciliopathies are a wide spectrum of human diseases, which are caused by perturbations in the function of primary cilia. Tooth enamel anomalies are often seen in ciliopathy patients; however, the role of primary cilia in enamel formation remains unclear. MATERIALS AND METHODS: We examined mice with epithelial conditional deletion of the ciliary protein, Ift88, (Ift88fl / fl ;K14Cre). RESULTS: Ift88fl / fl ;K14Cre mice showed premature abrasion in molars. A pattern of enamel rods which is determined at secretory stage, was disorganized in Ift88 mutant molars. Many amelogenesis-related molecules expressing at the secretory stage, including amelogenin and ameloblastin, enamelin, showed significant downregulation in Ift88 mutant molar tooth germs. Shh signaling is essential for amelogenesis, which was found to be downregulated in Ift88 mutant molar at the secretory stage. Application of Shh signaling agonist at the secretory stage partially rescued enamel anomalies in Ift88 mutant mice. CONCLUSION: Findings in the present study indicate that the function of the primary cilia via Ift88 is critical for the secretory stage of amelogenesis through involving Shh signaling.

    DOI: 10.1111/odi.14162

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  • Expression of R-spondins/Lgrs in development of movable craniofacial organs. Reviewed International journal

    Jun Nihara, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Fumiya Meguro, Takehisa Kudo, Supaluk Trakanant, Takahiro Nagai, Isao Saito, Takeyasu Maeda, Atsushi Ohazama

    Gene expression patterns : GEP   41   119195 - 119195   2021.9

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    Wnt signaling plays a critical role in the development of many organs, including the major movable craniofacial organs tongue, lip, and eyelid. Four members of the R-spondin family (Rspo1-4) bind to Lgr4/5/6 to regulate the activation of Wnt signaling. However, it is not fully understood how Rspos/Lgrs regulate Wnt signaling during the development of movable craniofacial organs. To address this question, we examined the expression of Rspos, Lgrs, and Axin2 (major mediator of canonical Wnt signaling) during tongue, lip, and eyelid development. The expression of Axin2, Rspos and Lgrs was observed in many similar regions, suggesting that Rspos likely activate canonical Wnt signaling through the Lgr-dependent pathway in these regions. Lgr expression was not detected in regions where Axin2 and Rspos were expressed, suggesting that Rspos might activate canonical Wnt signaling through the Lgr-independent pathway in these regions. In addition, the expression of Rspos and Lgrs were observed in some other regions where Axin2 was not expressed, suggesting the possibility that Rspos and/or Lgrs are involved in non-canonical Wnt signaling or the Wnt-independent pathway. Thus, we identified a dynamic spatiotemporal expression pattern of Rspos and Lgrs during the development of the eyelid, tongue, and lip.

    DOI: 10.1016/j.gep.2021.119195

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  • MicroRNAs regulate distal region of mandibular development through Hh signaling. Reviewed International journal

    Supaluk Trakanant, Jun Nihara, Takahiro Nagai, Maiko Kawasaki, Katsushige Kawasaki, Yoko Ishida, Fumiya Meguro, Takehisa Kudo, Akane Yamada, Takeyasu Maeda, Isao Saito, Atsushi Ohazama

    Journal of anatomy   238 ( 3 )   711 - 719   2021.3

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    Mandibular anomalies are often seen in various congenital diseases, indicating that mandibular development is under strict molecular control. Therefore, it is crucial to understand the molecular mechanisms involved in mandibular development. MicroRNAs (miRNAs) are noncoding small single-stranded RNAs that play a critical role in regulating the level of gene expression. We found that the mesenchymal conditional deletion of miRNAs arising from a lack of Dicer (an essential molecule for miRNA processing, Dicerfl/fl ;Wnt1Cre), led to an abnormal groove formation at the distal end of developing mandibles. At E10.5, when the region forms, inhibitors of Hh signaling, Ptch1 and Hhip1 showed increased expression at the region in Dicer mutant mandibles, while Gli1 (a major mediator of Hh signaling) was significantly downregulated in mutant mandibles. These suggest that Hh signaling was downregulated at the distal end of Dicer mutant mandibles by increased inhibitors. To understand whether the abnormal groove formation inDicer mutant mandibles was caused by the downregulation of Hh signaling, mice with a mesenchymal deletion of Hh signaling activity arising from a lack of Smo (an essential molecule for Hh signaling activation, Smofl/fl ;Wnt1Cre) were examined. Smofl/fl ;Wnt1Cre mice showed a similar phenotype in the distal region of their mandibles to those in Dicerfl/fl ;Wnt1Cre mice. We also found that approximately 400 miRNAs were expressed in wild-type mandibular mesenchymes at E10.5, and six microRNAs were identified as miRNAs with binding potential against both Ptch1 and Hhip1. Their expressions at the distal end of the mandible were confirmed by in situ hybridization. This indicates that microRNAs regulate the distal part of mandibular formation at an early stage of development by involving Hh signaling activity through controlling its inhibitor expression level.

    DOI: 10.1111/joa.13328

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  • Changes in signalling pathways in the palatal cleft in CL/Fr mice Reviewed

    Akane Yamada, Takahiro Nagai, Atsushi Kitamura, Maiko Kawasaki, Katsushige Kawasaki, Yasumitsu Kodama, Takeyasu Maeda, Atsushi Ohazama, Ritsuo Takagi

    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   32 ( 5 )   331 - 335   2020.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    Cleft palate is one of the most common congenital diseases. Therefore, elucidating the molecular mechanisms of palate development is crucial for basic science and the clinical field. Cleft palate in mouse with targeted gene mutation is an excellent experimental model for clarifying the mechanisms driving palate development. Cleft palate occurs in two forms: a cleft between the primary and secondary palates (primary cleft palate) and a cleft between the secondary palates (secondary cleft palate). It remains unclear whether primary palate development is under similar molecular control as secondary palate formation, since most of studies have focused on secondary palate development using mutant mice. CL/Fraser (CL/Fr) is a mouse strain that often exhibits spontaneous primary cleft palate; however, the molecular changes in primary cleft palate in CL/Fr mice are not fully understood. Several signaling pathways, including Shh, Fgf, Bmp, and Wnt signaling, have been shown to play key roles in secondary palate development. In the present study, we found that Shh and Wnt signaling pathways were downregulated in the primary cleft palate region in CL/Fr mice.

    DOI: 10.1016/j.ajoms.2019.12.001

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  • Overactivation of the NF-κB pathway impairs molar enamel formation. Reviewed International journal

    Akane Yamada, Maiko Kawasaki, Yasuo Miake, Yurie Yamada, James Blackburn, Kataushige Kawasaki, Supaluk Trakanant, Takahiro Nagai, Jun Nihara, Takehisa Kudo, Fumiya Meguro, Ruth Schmidt-Ullrich, Bigang Liu, Yinling Hu, Angustias Page, Ángel Ramírez, Paul T Sharpe, Takeyasu Maeda, Ritsuo Takagi, Atsushi Ohazama

    Oral diseases   26 ( 7 )   1513 - 1522   2020.5

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    OBJECTIVE: Hypohidrotic ectodermal dysplasia (HED) is a hereditary disorder characterized by abnormal structures and functions of the ectoderm-derived organs, including teeth. HED patients exhibit a variety of dental symptoms, such as hypodontia. Although disruption of the EDA/EDAR/EDARADD/NF-κB pathway is known to be responsible for HED, it remains unclear whether this pathway is involved in the process of enamel formation. EXPERIMENTAL SUBJECTS AND METHODS: To address this question, we examined the mice overexpressing Ikkβ (an essential component required for the activation of NF-κB pathway) under the keratin 5 promoter (K5-Ikkβ). RESULTS: Upregulation of the NF-κB pathway was confirmed in the ameloblasts of K5-Ikkβ mice. Premature abrasion was observed in the molars of K5-Ikkβ mice, which was accompanied by less mineralized enamel. However, no significant changes were observed in the enamel thickness and the pattern of enamel rods in K5-Ikkβ mice. Klk4 expression was significantly upregulated in the ameloblasts of K5-Ikkβ mice at the maturation stage, and the expression of its substrate, amelogenin, was remarkably reduced. This suggests that abnormal enamel observed in K5-Ikkβ mice was likely due to the compromised degradation of enamel protein at the maturation stage. CONCLUSION: Therefore, we could conclude that the overactivation of the NF-κB pathway impairs the process of amelogenesis.

    DOI: 10.1111/odi.13384

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  • Molecular mechanisms in palatal rugae development. Reviewed International journal

    Supaluk Trakanant, Jun Nihara, Maiko Kawasaki, Fumiya Meguro, Akane Yamada, Katsushige Kawasaki, Isao Saito, Maeda Takeyasu, Atsushi Ohazama

    Journal of oral biosciences   62 ( 1 )   30 - 35   2020.3

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    BACKGROUND: Periodic patterning of iterative structures is diverse across the animal kingdom. Clarifying the molecular mechanisms involved in the formation of these structures helps to elucidate the genetic commonality of developmental processes, as organs with these structures are believed to share the same molecular mechanisms and fundamental processes. Palatal rugae are periodic corrugated structures on the hard palate and are conserved in all mammals. Although the numbers and patterns of the palatal rugae are species specific, they are consistent in each mammalian species, except humans. HIGHLIGHT: Palatal rugae development is thus under strict genetic control in most mammals and is an excellent model to investigate the genetic commonality of developmental processes to form periodic patterning. CONCLUSION: This review highlights the current understanding of the molecular mechanisms of palatal rugae development.

    DOI: 10.1016/j.job.2019.12.002

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  • Ift88 is involved in mandibular development. Reviewed International journal

    Atsushi Kitamura, Maiko Kawasaki, Katsushige Kawasaki, Fumiya Meguro, Akane Yamada, Takahiro Nagai, Yasumitsu Kodama, Supaluk Trakanant, Paul T Sharpe, Takeyasu Maeda, Ritsuo Takagi, Atsushi Ohazama

    Journal of anatomy   236 ( 2 )   317 - 324   2020.2

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    The mandible is a crucial organ in both clinical and biological fields due to the high frequency of congenital anomalies and the significant morphological changes during evolution. Primary cilia play a critical role in many biological processes, including the determination of left/right axis patterning, the regulation of signaling pathways, and the formation of bone and cartilage. Perturbations in the function of primary cilia are known to cause a wide spectrum of human diseases: the ciliopathies. Craniofacial dysmorphologies, including mandibular deformity, are often seen in patients with ciliopathies. Mandibular development is characterized by chondrogenesis and osteogenesis; however, the role of primary cilia in mandibular development is not fully understood. To address this question, we generated mice with mesenchymal deletions of the ciliary protein, Ift88 (Ift88fl/fl ;Wnt1Cre). Ift88fl/fl ;Wnt1Cre mice showed ectopic mandibular bone formation, whereas Ift88 mutant mandible was slightly shortened. Meckel's cartilage was modestly expanded in Ift88fl/fl ;Wnt1Cre mice. The downregulation of Hh signaling was found in most of the mesenchyme of Ift88 mutant mandible. However, mice with a mesenchymal deletion of an essential molecule for Hh signaling activity, Smo (Smofl/fl ;Wnt1Cre), showed only ectopic mandibular formation, whereas Smo mutant mandible was significantly shortened. Ift88 is thus involved in chondrogenesis and osteogenesis during mandibular development, partially through regulating Sonic hedgehog (Shh) signaling.

    DOI: 10.1111/joa.13096

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  • Primary cilia in murine palatal rugae development. Reviewed International journal

    Mayuko Nakaniwa, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Fumiya Meguro, Maeda Takeyasu, Atsushi Ohazama

    Gene expression patterns : GEP   34   119062 - 119062   2019.12

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    Periodic patterning of iterative structures is a fundamental process during embryonic development, since these structures are diverse across the animal kingdom. Therefore, elucidating the molecular mechanisms in the formation of these structures promotes understanding of the process of organogenesis. Periodically patterned ridges, palatal rugae (situated on the hard palate of mammals), are an excellent experimental model to clarify the molecular mechanisms involved in the formation of periodic patterning of iterative structures. Primary cilia are involved in many biological events, including the regulation of signaling pathways such as Shh and non-canonical Wnt signaling. However, the role of primary cilia in the development of palatal rugae remains unclear. We found that primary cilia were localized to the oral cavity side of the interplacode epithelium of the palatal rugae, whereas restricted localization of primary cilia could not be detected in other regions. Next, we generated mice with a placodal conditional deletion of the primary cilia protein Ift88, using ShhCre mice (Ift88 fl/fl;ShhCre). Highly disorganized palatal rugae were observed in Ift88 fl/fl;ShhCre mice. Furthermore, by comparative in situ hybridization analysis, many Shh and non-canonical Wnt signaling-related molecules showed spatiotemporal expression patterns during palatal rugae development, including restricted expression in the epithelium (placodes and interplacodes) and mesenchyme. Some of these expression were found to be altered in Ift88 fl/fl;ShhCre mice. Primary cilia is thus involved in development of palatal rugae.

    DOI: 10.1016/j.gep.2019.119062

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  • Bmp signaling in molar cusp formation. Reviewed International journal

    Fumiya Meguro, Thantrira Porntaveetus, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Yoshito Kakihara, Makio Saeki, Koichi Tabeta, John A Kessler, Takeyasu Maeda, Atsushi Ohazama

    Gene expression patterns : GEP   32   67 - 71   2019.6

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    Tooth cusp is a crucial structure, since the shape of the molar tooth is determined by number, shape, and size of the cusp. Bone morphogenetic protein (Bmp) signaling is known to play a critical role in tooth development, including in initiation. However, it remains unclear whether Bmp signaling is also involved in cusp formation. To address this question, we examined cusp in two different transgenic mouse lines: mice with overexpression of Bmp4 (K14-Bmp4), and those with Bmp inhibitor, Noggin, (K14-Noggin) under keratin14 (K14) promoter. K14-Noggin mice demonstrated extra cusps, whereas reduced number of cusps was observed in K14-Bmp4 mice. To further understand how Bmps are expressed during cusp formation, we performed whole-mount in situ hybridisation analysis of three major Bmps (Bmp2, Bmp4, and Bmp7) in murine maxillary and mandibular molars from E14.5 to P3. The linear expressions of Bmp2 and Bmp4 were observed in both maxillary and mandibular molars at E14.5. The expression patterns of Bmp2 and Bmp4 became significantly different between the maxillary and mandibular molars at E16.5. At P3, all Bmps were expressed in all the cusp regions of the maxillary molar; however, the patterns differed. All Bmps thus exhibited dynamic temporo-spatial expression during the cusp formation. It could therefore be inferred that Bmp signaling is involved in regulating cusp formation.

    DOI: 10.1016/j.gep.2019.04.002

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  • Ift88 limits bone formation in maxillary process through suppressing apoptosis. Reviewed International journal

    Momoko Watanabe, Maiko Kawasaki, Katsushige Kawasaki, Atsushi Kitamura, Takahiro Nagai, Yasumitsu Kodama, Fumiya Meguro, Akane Yamada, Paul T Sharpe, Takeyasu Maeda, Ritsuo Takagi, Atsushi Ohazama

    Archives of oral biology   101   43 - 50   2019.5

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    OBJECTIVE: The development of the maxillary bone is under strict molecular control because of its complicated structure. Primary cilia play a critical role in craniofacial development, since defects in primary cilia are known to cause congenital craniofacial dysmorphologies as a wide spectrum of human diseases: the ciliopathies. The primary cilia also are known to regulate bone formation. However, the role of the primary cilia in maxillary bone development is not fully understood. DESIGN: To address this question, we generated mice with a mesenchymal conditional deletion ofIft88 using the Wnt1Cre mice (Ift88fl/fl;Wnt1Cre). The gene Ift88 encodes a protein that is required for the function and formation of primary cilia. RESULTS: It has been shown thatIft88fl/fl;Wnt1Cre mice exhibit cleft palate. Here, we additionally observed excess bone formation in the Ift88 mutant maxillary process. We also found ectopic apoptosis in the Ift88 mutant maxillary process at an early stage of development. To investigate whether the ectopic apoptosis is related to the Ift88 mouse maxillary phenotypes, we generated Ift88fl/fl;Wnt1Cre;p53-/- mutants to reduce apoptosis. The Ift88fl/fl;Wnt1Cre;p53-/- mice showed no excess bone formation, suggesting that the cells evading apoptosis by the presence of Ift88 in wild-type mice limit bone formation in maxillary development. On the other hand, the palatal cleft was retained in the Ift88fl/fl;Wnt1Cre;p53-/- mice, indicating that the excess bone formation or abnormal apoptosis was independent of the cleft palate phenotype in Ift88 mutant mice. CONCLUSIONS: Ift88 limits bone formation in the maxillary process by suppressing apoptosis.

    DOI: 10.1016/j.archoralbio.2019.02.017

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  • MicroRNAs control eyelid development through regulating Wnt signaling. Reviewed International journal

    Takahiro Nagai, Supaluk Trakanant, Maiko Kawasaki, Katsushige Kawasaki, Yurie Yamada, Momoko Watanabe, James Blackburn, Yoko Otsuka-Tanaka, Mitsue Hishinuma, Atsushi Kitatmura, Fumiya Meguro, Akane Yamada, Yasumitsu Kodama, Takeyasu Maeda, Qiliang Zhou, Yasuo Saijo, Akihiro Yasue, Paul T Sharpe, Robert Hindges, Ritsuo Takagi, Atsushi Ohazama

    Developmental dynamics : an official publication of the American Association of Anatomists   248 ( 3 )   201 - 210   2019.3

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    BACKGROUND: The timing, location, and level of gene expression are crucial for normal organ development, because morphogenesis requires strict genetic control. MicroRNAs (miRNAs) are noncoding small single-stranded RNAs that play a critical role in regulating gene expression level. Although miRNAs are known to be involved in many biological events, the role of miRNAs in organogenesis is not fully understood. Mammalian eyelids fuse and separate during development and growth. In mice, failure of this process results in the eye-open at birth (EOB) phenotype. RESULTS: It has been shown that conditional deletion of mesenchymal Dicer (an essential protein for miRNA processing; Dicer fl/fl ;Wnt1Cre) leads to the EOB phenotype with full penetrance. Here, we identified that the up-regulation of Wnt signaling resulted in the EOB phenotype in Dicer mutants. Down-regulation of Fgf signaling observed in Dicer mutants was caused by an inverse relationship between Fgf and Wnt signaling. Shh and Bmp signaling were down-regulated as the secondary effects in Dicer fl/fl ;Wnt1Cre mice. Wnt, Shh, and Fgf signaling were also found to mediate the epithelial-mesenchymal interactions in eyelid development. CONCLUSIONS: miRNAs control eyelid development through Wnt. Developmental Dynamics 248:201-210, 2019. © 2019 Wiley Periodicals, Inc.

    DOI: 10.1002/dvdy.10

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  • Lrp4/Wise regulates palatal rugae development through Turing-type reaction-diffusion mechanisms. Reviewed International journal

    Maiko Kawasaki, Katsushige Kawasaki, Fumiya Meguro, Akane Yamada, Ryuichi Ishikawa, Thantrira Porntaveetus, James Blackburn, Yoko Otsuka-Tanaka, Naoaki Saito, Masato S Ota, Paul T Sharpe, John A Kessler, Joachim Herz, Martyn T Cobourne, Takeyasu Maeda, Atsushi Ohazama

    PloS one   13 ( 9 )   e0204126   2018

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    Periodic patterning of iterative structures is diverse across the animal kingdom. Clarifying the molecular mechanisms involved in the formation of these structure helps to elucidate the process of organogenesis. Turing-type reaction-diffusion mechanisms have been shown to play a critical role in regulating periodic patterning in organogenesis. Palatal rugae are periodically patterned ridges situated on the hard palate of mammals. We have previously shown that the palatal rugae develop by a Turing-type reaction-diffusion mechanism, which is reliant upon Shh (as an inhibitor) and Fgf (as an activator) signaling for appropriate organization of these structures. The disturbance of Shh and Fgf signaling lead to disorganized palatal rugae. However, the mechanism itself is not fully understood. Here we found that Lrp4 (transmembrane protein) was expressed in a complementary pattern to Wise (a secreted BMP antagonist and Wnt modulator) expression in palatal rugae development, representing Lrp4 expression in developing rugae and Wise in the inter-rugal epithelium. Highly disorganized palatal rugae was observed in both Wise and Lrp4 mutant mice, and these mutants also showed the downregulation of Shh signaling, which was accompanied with upregulation of Fgf signaling. Wise and Lrp4 are thus likely to control palatal rugae development by regulating reaction-diffusion mechanisms through Shh and Fgf signaling. We also found that Bmp and Wnt signaling were partially involved in this mechanism.

    DOI: 10.1371/journal.pone.0204126

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  • Expanding the Oro-Dental and Mutational Spectra of Kabuki Syndrome and Expression of KMT2D and KDM6A in Human Tooth Germs. Reviewed International journal

    Thantrira Porntaveetus, Mushriq F Abid, Thanakorn Theerapanon, Chalurmpon Srichomthong, Atsushi Ohazama, Katsushige Kawasaki, Maiko Kawasaki, Kanya Suphapeetiporn, Paul T Sharpe, Vorasuk Shotelersuk

    International journal of biological sciences   14 ( 4 )   381 - 389   2018

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    Kabuki syndrome is a rare genetic disorder characterized by distinct dysmorphic facial features, intellectual disability, and multiple developmental abnormalities. Despite more than 350 documented cases, the oro-dental spectrum associated with kabuki syndrome and expression of KMT2D (histone-lysine N-methyltransferase 2D) or KDM6A (lysine-specific demethylase 6A) genes in tooth development have not been well defined. Here, we report seven unrelated Thai patients with Kabuki syndrome having congenital absence of teeth, malocclusion, high-arched palate, micrognathia, and deviated tooth shape and size. Exome sequencing successfully identified that six patients were heterozygous for mutations in KMT2D, and one in KDM6A. Six were novel mutations, of which five were in KMT2D and one in KDM6A. They were truncating mutations including four frameshift deletions and two nonsense mutations. The predicted non-functional KMT2D and KDM6A proteins are expected to cause disease by haploinsufficiency. Our study expands oro-dental, medical, and mutational spectra associated with Kabuki syndrome. We also demonstrate for the first time that KMT2D and KDM6A are expressed in the dental epithelium of human tooth germs.

    DOI: 10.7150/ijbs.23517

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  • Sox Genes Show Spatiotemporal Expression during Murine Tongue and Eyelid Development. Reviewed International journal

    Ryuichi Ishikawa, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Supaluk Trakanant, Fumiya Meguro, Atsushi Kitamura, Takehisa Kudo, Takeyasu Maeda, Atsushi Ohazama

    International journal of dentistry   2018   1601363 - 1601363   2018

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    The tongue is a critical organ, involved in functions such as speaking, swallowing, mastication, and degustation. Although Sox genes are known to play critical roles in many biological processes, including organogenesis, the expression of the Sox family members during tongue development remains unclear. We therefore performed a comparative in situ hybridization analysis of 17 Sox genes (Sox1-14, 17, 18, and 21) during murine tongue development. Sox2, 4, 6, 8, 9, 10, 11, 12, and 21 were found to be expressed in the tongue epithelium, whereas Sox2, 4-6, 8-11, 13, and 21 showed expression in the mesenchyme of the developing tongue. Expression of Sox1, 4, 6, 8-12, and 21 were observed in the developing tongue muscle. Sox5 and 13 showed expression only at E12, while Sox1 expression was observed only on E18. Sox6, 8, 9, and 12 showed expression at several stages. Although the expression of Sox2, 4, 10, 11, and 21 was detected during all the four stages of tongue development, their expression patterns differed among the stages. We thus identified a dynamic spatiotemporal expression pattern of the Sox genes during murine tongue development. To understand whether Sox genes are involved in the development of other craniofacial organs through similar roles to those in tongue development, we also examined the expression of Sox genes in eyelid primordia, which also contain epithelium, mesenchyme, and muscle. However, expression patterns and timing of Sox genes differed between tongue and eyelid development. Sox genes are thus related to organogenesis through different functions in each craniofacial organ.

    DOI: 10.1155/2018/1601363

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  • Regional Regulation of Filiform Tongue Papillae Development by Ikk alpha/Irf6 Reviewed

    Maiko Kawasaki, Katsushige Kawasaki, Shelly Oommen, James Blackburn, Momoko Watanabe, Takahiro Nagai, Atsushi Kitamura, Takeyasu Maeda, Bigang Liu, Ruth Schmidt-Ullrich, Taishin Akiyama, Jun-Ichiro Inoue, Nigel L. Hammond, Paul T. Sharpe, Atsushi Ohazama

    DEVELOPMENTAL DYNAMICS   245 ( 9 )   937 - 946   2016.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    Background: Non-gustatory filiform papillae play critical roles in helping to grip food, drawing food to the esophagus, cleaning the mouth, and spreading saliva. The molecular mechanisms of filiform tongue papillae development however are not fully understood. Results: We found Ikk alpha and Irf6 expression in developing tongue epithelium, and describe here specific tongue abnormalities in mice with mutation of these genes, indicating a role for Ikk alpha and Irf6 in filiform papillae development. Ikk alpha and Irf6 mutant tongues showed ectopic vertical epithelium at the midline, while lateral sides of mutant tongues adhered to the oral mucosa. Both the ectopic median vertical epithelium and adhered epithelium exhibited the presence of filiform tongue papillae, whereas epithelium between the median vertical epithelium and adhered tongue showed a loss of filiform tongue papillae. Timing of filiform papillae development was found to be slightly different between the midline and lateral regions of the wild-type tongue. Conclusions: Filiform papillae thus develop through distinct molecular mechanisms between the regions of tongue dorsum in the medio-lateral axis, with some filiform papillae developing under the control of Ikk alpha and Irf6. Developmental Dynamics 245: 937-946, 2016. (C) 2016 Wiley Periodicals, Inc.

    DOI: 10.1002/DVDY.24427

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  • Spatio-temporal expression of Sox genes in murine palatogenesis Reviewed

    Momoko Watanabe, Katsushige Kawasaki, Maiko Kawasaki, Thantrira Portaveetus, Shelly Oommen, James Blackburn, Takahiro Nagai, Atsushi Kitamura, Atsushi Nishikawa, Yasumitsu Kodama, Ritsuo Takagi, Takeyasu Maeda, Paul T. Sharpe, Atsushi Ohazama

    GENE EXPRESSION PATTERNS   21 ( 2 )   111 - 118   2016.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    Members of the Sox gene family play critical roles in many biological processes including organogenesis. We carried out comparative in situ hybridisation analysis of seventeen Sox genes (Sox1-14, 17, 18 and 21) during murine palatogenesis from initiation to fusion of the palatal shelves above the dorsal side of the tongue. At palatal shelf initiation (E12.5), the localized expression of six Sox genes (Sox2, 5, 6, 9,12 and 13) was observed in the shelves, whereas Sox4 and Sox11 showed ubiquitious expression. During the down growth of palatal shelves (E13.5), Sox4, Sox5, and Sox9 exhibited restricted expression to the interior side of the palatal shelves facing the tongue. Following elevation of the palatal shelves (E14.5), Sox2, Sox11 and Sox21 expression was present in the midline epithelial seam. We thus identify dynamic spatio-temporal expression of Sox gene family during the process of palatogenesis. (C) 2016 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.gep.2016.05.002

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  • Excess NF-kappa B Induces Ectopic Odontogenesis in Embryonic Incisor Epithelium Reviewed

    J. Blackburn, K. Kawasaki, T. Porntaveetus, M. Kawasaki, Y. Otsuka-Tanaka, Y. Miake, M. S. Ota, M. Watanabe, M. Hishinuma, T. Nomoto, S. Oommen, S. Ghafoor, F. Harada, K. Nozawa-Inoue, T. Maeda, R. Peterkova, H. Lesot, J. Inoue, T. Akiyama, R. Schmidt-Ullrich, B. Liu, Y. Hu, A. Page, A. Ramirez, P. T. Sharpe, A. Ohazama

    JOURNAL OF DENTAL RESEARCH   94 ( 1 )   121 - 128   2015.1

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    Nuclear factor kappa B (NF-kappa B) signaling plays critical roles in many physiological and pathological processes, including regulating organogenesis. Down-regulation of NF-kappa B signaling during development results in hypohidrotic ectodermal dysplasia. The roles of NF-kappa B signaling in tooth development, however, are not fully understood. We examined mice overexpressing IKK beta, an essential component of the NF-kappa B pathway, under keratin 5 promoter (K5-Ikk beta). K5-Ikk beta mice showed supernumerary incisors whose formation was accompanied by up-regulation of canonical Wnt signaling. Apoptosis that is normally observed in wild-type incisor epithelium was reduced in K5-Ikk beta mice. The supernumerary incisors in K5-Ikk beta mice were found to phenocopy extra incisors in mice with mutations of Wnt inhibitor, Wise. Excess NF-kappa B activity thus induces an ectopic odontogenesis program that is usually suppressed under physiological conditions.

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  • Expression of Sox genes in tooth development Reviewed

    Katsushige Kawasaki, Maiko Kawasaki, Momoko Watanabe, Erik Idrus, Takahiro Nagai, Shelly Oommen, Takeyasu Maeda, Nobuko Hagiwara, Jianwen Que, Paul T. Sharpe, Atsushi Ohazama

    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY   59 ( 10-12 )   471 - 478   2015

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    Members of the Sox gene family play roles in many biological processes including organogenesis. We carried out comparative in situ hybridization analysis of seventeen sox genes (Sox 1-14, 17, 18, 21) during murine odontogenesis from the epithelial thickening to the cytodifferentiation stages. Localized expression of five Sox genes (Sox6, 9, 13, 14 and 21) was observed in tooth bud epithelium. Sox13 showed restricted expression in the primary enamel knots. At the early bell stage, three Sox genes (Sox8, 11, 17 and 21) were expressed in pre-ameloblasts, whereas two others (Sox5 and 18) showed expression in odontoblasts. Sox genes thus showed a dynamic spatio-temporal expression during tooth development.

    DOI: 10.1387/ijdb.150192ao

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  • Analysis of Oral Moisturizing Effects of Trehalose Tablets. Reviewed

    Kawasaki M, Suzuki A, Sakai Y, Sohda M, Igarashi A, Watanabe T

    NIIGATA DENTAL JOURNAL   45 ( 2 )   13 - 20   2015

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  • R-spondins/Lgrs expression in tooth development Reviewed

    Maiko Kawasaki, Thantrira Porntaveetus, Katsushige Kawasaki, Shelly Oommen, Yoko Otsuka-Tanaka, Mitsue Hishinuma, Takato Nomoto, Takeyasu Maeda, Keiyo Takubo, Toshio Suda, Paul T. Sharpe, Atsushi Ohazama

    DEVELOPMENTAL DYNAMICS   243 ( 6 )   844 - 851   2014.6

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    Background: Tooth development is highly regulated in mammals and it is regulated by networks of signaling pathways (e. g. Tnf, Wnt, Shh, Fgf and Bmp) whose activities are controlled by the balance between ligands, activators, inhibitors and receptors. The members of the R-spondin family are known as activators of Wnt signaling, and Lgr4, Lgr5, and Lgr6 have been identified as receptors for R-spondins. The role of R-spondin/Lgr signaling in tooth development, however, remains unclear. Results: We first carried out comparative in situ hybridization analysis of R-spondins and Lgrs, and identified their dynamic spatio-temporal expression in murine odontogenesis. R-spondin2 expression was found both in tooth germs and the tooth-less region, the diastema. We further examined tooth development in R-spondin2 mutant mice, and although molars and incisors exhibited no significant abnormalities, supernumerary teeth were observed in the diastema. Conclusions: R-spondin/Lgr signaling is thus involved in tooth development. Developmental Dynamics 243:844-851, 2014. (c) 2014 Wiley Periodicals, Inc.

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  • PKA regulatory subunit expression in tooth development Reviewed

    Silvia Ferreira de Sousa, Katsushige Kawasaki, Maiko Kawasaki, Ana Angelova Volponi, Ricardo Santiago Gomez, Carolina Cavalieri Gomes, Paul T. Sharpe, Atsushi Ohazama

    GENE EXPRESSION PATTERNS   15 ( 1 )   46 - 51   2014.5

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    Protein kinase A (PK A) plays critical roles in many biological processes including cell proliferation, cell differentiation, cellular metabolism and gene regulation. Mutation in PKA regulatory subunit, PRKAR1A has previously been identified in odontogenic myxomas, but it is unclear whether PKA is involved in tooth development. The aim of the present study was to assess the expression of alpha isoforms of PICA regulatory subunit (Prkar1a and Prkar2a) in mouse and human odontogenesis by in situ hybridization. PRKAR1A and PRKAR2A mRNA transcription was further confirmed in a human deciduous germ by qRT-PCR. Mouse Prkar1a and human PRKAR2A exhibited a dynamic spatio-temporal expression in tooth development, whereas neither human PRKAR1A nor mouse Prkar2a showed their expression in odontogenesis. These isoforms thus showed different expression pattern between human and mouse tooth germs. (C) 2014 Elsevier B.V. All rights reserved.

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  • Multiple postnatal craniofacial anomalies are characterized by conditional loss of polycystic kidney disease 2 (Pkd2) Reviewed

    Roman H. Khonsari, Atsushi Ohazama, Ramin Raouf, Maiko Kawasaki, Katsushige Kawasaki, Thantrira Porntaveetus, Sarah Ghafoor, Peter Hammond, Michael Suttie, Guillaume A. Odri, Richard N. Sandford, John N. Wood, Paul T. Sharpe

    HUMAN MOLECULAR GENETICS   22 ( 9 )   1873 - 1885   2013.5

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    Polycystin 2 (Pkd2), which belongs to the transient receptor potential family, plays a critical role in development. Pkd2 is mainly localized in the primary cilia, which also function as mechanoreceptors in many cells that influence multiple biological processes including Ca-2 influx, chemical activity and signalling pathways. Mutations in many cilia proteins result in craniofacial abnormalities. Orofacial tissues constantly receive mechanical forces and are known to develop and grow through intricate signalling pathways. Here we investigate the role of Pkd2, whose role remains unclear in craniofacial development and growth. In order to determine the role of Pkd2 in craniofacial development, we located expression in craniofacial tissues and analysed mice with conditional deletion of Pkd2 in neural crest-derived cells, using Wnt1Cre mice. Pkd2 mutants showed many signs of mechanical trauma such as fractured molar roots, distorted incisors, alveolar bone loss and compressed temporomandibular joints, in addition to abnormal skull shapes. Significantly, mutants showed no indication of any of these phenotypes at embryonic stages when heads perceive no significant mechanical stress in utero. The results suggest that Pkd2 is likely to play a critical role in craniofacial growth as a mechanoreceptor. Pkd2 is also identified as one of the genes responsible for autosomal dominant polycystic kidney disease (ADPKD). Since facial anomalies have never been identified in ADPKD patients, we carried out three-dimensional photography of patient faces and analysed these using dense surface modelling. This analysis revealed specific characteristics of ADPKD patient faces, some of which correlated with those of the mutant mice.

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  • Adult Human Gingival Epithelial Cells as a Source for Whole-tooth Bioengineering Reviewed

    A. Angelova Volponi, M. Kawasaki, P. T. Sharpe

    JOURNAL OF DENTAL RESEARCH   92 ( 4 )   329 - 334   2013.4

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    Teeth develop from interactions between embryonic oral epithelium and neural-crest-derived mesenchyme. These cells can be separated into single-cell populations and recombined to form normal teeth, providing a basis for bioengineering new teeth if suitable, non-embryonic cell sources can be identified. We show here that cells can be isolated from adult human gingival tissue that can be expanded in vitro and, when combined with mouse embryonic tooth mesenchyme cells, form teeth. Teeth with developing roots can be produced from this cell combination following transplantation into renal capsules. These bioengineered teeth contain dentin and enamel with ameloblast-like cells and rests of Malassez of human origin.

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  • The buccohypophyseal canal is an ancestral vertebrate trait maintained by modulation in sonic hedgehog signaling Reviewed

    Roman H. Khonsari, Maisa Seppala, Alan Pradel, Hugo Dutel, Gael Clement, Oleg Lebedev, Sarah Ghafoor, Michaela Rothova, Abigael Tucker, John G. Maisey, Chen-Ming Fan, Maiko Kawasaki, Atsushi Ohazama, Paul Tafforeau, Brunella Franco, Jill Helms, Courtney J. Haycraft, Albert David, Philippe Janvier, Martyn T. Cobourne, Paul T. Sharpe

    BMC BIOLOGY   11   2013.3

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    Background: The pituitary gland is formed by the juxtaposition of two tissues: neuroectoderm arising from the basal diencephalon, and oral epithelium, which invaginates towards the central nervous system from the roof of the mouth. The oral invagination that reaches the brain from the mouth is referred to as Rathke's pouch, with the tip forming the adenohypophysis and the stalk disappearing after the earliest stages of development. In tetrapods, formation of the cranial base establishes a definitive barrier between the pituitary and oral cavity; however, numerous extinct and extant vertebrate species retain an open buccohypophyseal canal in adulthood, a vestige of the stalk of Rathke's pouch. Little is currently known about the formation and function of this structure. Here we have investigated molecular mechanisms driving the formation of the buccohypophyseal canal and their evolutionary significance.
    Results: We show that Rathke's pouch is located at a boundary region delineated by endoderm, neural crest-derived oral mesenchyme and the anterior limit of the notochord, using CD1, R26R-Sox17-Cre and R26R-Wnt1-Cre mouse lines. As revealed by synchrotron X-ray microtomography after iodine staining in mouse embryos, the pouch has a lobulated three-dimensional structure that embraces the descending diencephalon during pituitary formation. Polaris(fl/fl); Wnt1-Cre, Ofd1(-/-) and Kif3a(-/-) primary cilia mouse mutants have abnormal sonic hedgehog (Shh) signaling and all present with malformations of the anterior pituitary gland and midline structures of the anterior cranial base. Changes in the expressions of Shh downstream genes are confirmed in Gas1(-/-) mice. From an evolutionary perspective, persistence of the buccohypophyseal canal is a basal character for all vertebrates and its maintenance in several groups is related to a specific morphology of the midline that can be related to modulation in Shh signaling.
    Conclusion: These results provide insight into a poorly understood ancestral vertebrate structure. It appears that the opening of the buccohypophyseal canal depends upon Shh signaling and that modulation in this pathway most probably accounts for its persistence in phylogeny.

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  • The buccohypophyseal canal is an ancestral vertebrate trait maintained by modulation in sonic hedgehog signaling. Reviewed

    Khonsari RH, Seppala M, Pradel A, Dutel H, Clément G, Lebedev O, Ghafoor S, Rothova M, Tucker A, Maisey JG, Fan CM, Kawasaki M, Ohazama A, Tafforeau P, Franco B, Helms J, Haycraft CJ, David A, Janvier P, Cobourne MT, Sharpe PT

    BMC biology   11   27   2013.3

  • Oral Lining Mucosa Development Depends on Mesenchymal microRNAs Reviewed

    Otsuka-Tanaka Y, Oommen S, Kawasaki M, Kawasaki K, Imam N, Jalani-Ghazani F, Hindges R, Sharpe PT, Ohazama A

    J Dent Res.   92 ( (3) )   229 - 234   2012.12

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  • Distinct roles of MicroRNAs in epithelium and mesenchyme during tooth development Reviewed

    Shelly Oommen, Yoko Otsuka-Tanaka, Najam Imam, Maiko Kawasaki, Katsushige Kawasaki, Farnoosh Jalani-Ghazani, Angela Anderegg, Rajeshwar Awatramani, Robert Hindges, Paul T. Sharpe, Atsushi Ohazama

    DEVELOPMENTAL DYNAMICS   241 ( 9 )   1465 - 1472   2012.9

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    Background: Tooth development is known to be mediated by the cross-talk between signaling pathways, including Shh, Fgf, Bmp, and Wnt. MicroRNAs (miRNAs) are 19- to 25-nt noncoding small single-stranded RNAs that negatively regulate gene expression by binding target mRNAs, which is believed to be important for the fine-tuning signaling pathways in development. To investigate the role of miRNAs in tooth development, we examined mice with either mesenchymal (Wnt1Cre/Dicerfl/fl) or epithelial (ShhCre/Dicerfl/fl) conditional deletion of Dicer, which is essential for miRNA processing. Results: By using a CD1 genetic background for Wnt1Cre/Dicerfl/fl, we were able to examine tooth development, because the mutants retained mandible and maxilla primordia. Wnt1Cre/Dicerfl/fl mice showed an arrest or absence of teeth development, which varied in frequency between incisors and molars. Extra incisor tooth formation was found in ShhCre/Dicerfl/fl mice, whereas molars showed no significant anomalies. Microarray and in situ hybridization analysis identified several miRNAs that showed differential expression between incisors and molars. Conclusion: In tooth development, miRNAs thus play different roles in epithelium and mesenchyme, and in incisors and molars. Developmental Dynamics 241:1465-1472, 2012. (c) 2012 Wiley Periodicals, Inc.

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  • Cytoplasmic Plaque Formation in Hemidesmosome Development Is Dependent on SoxF Transcription Factor Function Reviewed

    Shelly Oommen, Mathias Francois, Maiko Kawasaki, Melanie Murrell, Katsushige Kawasaki, Thantrira Porntaveetus, Sarah Ghafoor, Neville J. Young, Yoshimasa Okamatsu, John McGrath, Peter Koopman, Paul T. Sharpe, Atsushi Ohazama

    PLOS ONE   7 ( 9 )   e43857   2012.9

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    Hemidesmosomes are composed of intricate networks of proteins, that are an essential attachment apparatus for the integrity of epithelial tissue. Disruption leads to blistering diseases such as epidermolysis bullosa. Members of the Sox gene family show dynamic and diverse expression patterns during development and mutation analyses in humans and mice provide evidence that they play a remarkable variety of roles in development and human disease. Previous studies have established that the mouse mutant ragged-opossum (Ra-op) expresses a dominant-negative form of the SOX18 transcription factor that interferes with the function of wild type SOX18 and of the related SOXF-subgroup proteins SOX7 and -17. Here we show that skin and oral mucosa in homozygous Ra-op mice display extensive detachment of epithelium from the underlying mesenchymal tissue, caused by tearing of epithelial cells just above the plasma membrane due to hemidesmosome disruption. In addition, several hemidesmosome proteins expression were found to be dysregulated in the Ra-op mice. Our data suggest that SOXF transcription factors play a role in regulating formation of cytoplasmic plaque protein assembly, and that disrupted SOXF function results in epidermolysis bullosa-like skin phenotypes.

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  • Evaluation of newly developed devices for denture placement and removal in the dependent elderly Reviewed

    Maiko Kawasaki, Shuichi Nomura, Naoto Okada, Akiko Nomura, Katsumi Uoshima

    GERODONTOLOGY   29 ( 2 )   E703 - E709   2012.6

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    Objective: The purpose of this study was to subjectively evaluate the utility of newly developed denture placement and removal devices. Objective observations were also made to support the evidence.
    Materials and method: Twenty-one subjects were instructed to place and remove their dentures with and without the devices. We evaluated the device based on a questionnaire. Objective observations were based on a 2-D image analysis. We analysed three factors: the time, the area and the circumference required to insert and remove the dentures.
    Results: Image analysis showed that the effectiveness and ease with which the subject used the device significantly improved with practice. The questionnaire data showed that a majority of the subjects appreciated the device after the first and second time. While there was no significant decrease in time required to place and remove dentures even with the device, the area and circumference of the movement on 2-D images were significantly reduced.
    Conclusion: In this study, the utility of denture placement and removal devices was evaluated both subjectively and objectively. Our data reveal that the device is effective in the elderly. Further minor improvement in the device might be required to increase its effectiveness.

    DOI: 10.1111/j.1741-2358.2011.00547.x

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  • Bmp signalling in filiform tongue papillae development Reviewed

    Katsushige Kawasaki, Thantrira Porntaveetus, Shelly Oommen, Sarah Ghafoor, Maiko Kawasaki, Yoko Otsuka-Tanaka, James Blackburn, John A. Kessler, Paul T. Sharpe, Atsushi Ohazama

    ARCHIVES OF ORAL BIOLOGY   57 ( 6 )   805 - 813   2012.6

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    Objective: Tongue papillae are critical organs in mastication. There are four different types of tongue papillae; fungiform, circumvallate, foliate, and filiform papillae. Unlike the other three taste papillae, non-gustatory papillae, filiform papillae cover the entire dorsal surface of the tongue and are important structures for the mechanical stress of sucking. Filiform papillae are further classified into two subtypes with different morphologies, depending on their location on the dorsum of the tongue. The filiform papillae at the intermolar eminence have pointed tips, whereas filiforrn papillae with rounded tips are found in other regions (anterior tongue). It remains unknown how the shape of each type of filiform papillae are determined during their development. Bmp signalling pathway has been known to regulate mechanisms that determine the shapes of many ectodermal organs. The aim of this study was to investigate the role of Bmp signalling in filiform papillae development.
    Design: Comparative in situ hybridization analysis of six Bmps (Bmp2-Bmp7) and two Bmpr genes (Bmpr1a and Bmpr1b) were carried out in filiform papillae development. We further examined tongue papillae in mice over-expressing Noggin under the keratin14 promoter (K14-Noggin).
    Results: We identified a dynamic temporo-spatial expression of Bmps in filiform papillae development. The K14-Noggin mice showed pointed filiform papillae in regions of the tongue normally occupied by the rounded type.
    Conclusions: Bmp signalling thus regulates the shape of filiform papillae. (C) 2011 Elsevier Ltd. All rights reserved.

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  • The role of Irf6 in tooth epithelial invagination Reviewed

    James Blackburn, Atsushi Ohazama, Katsushige Kawasaki, Yoko Otsuka-Tanaka, Bigang Liu, Kenya Honda, Ryan B. Rountree, Yinling Hu, Maiko Kawasaki, Walter Birchmeier, Ruth Schmidt-Ullrich, Akira Kinoshita, Brian C. Schutte, Nigel L. Hammond, Michael J. Dixon, Paul T. Sharpe

    DEVELOPMENTAL BIOLOGY   365 ( 1 )   61 - 70   2012.5

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    Thickening and the subsequent invagination of the epithelium are an important initial step in Ectodermal organ development. Ikk alpha has been shown to play a critical role in controlling epithelial growth, since Ikk alpha mutant mice show protrusions (evaginations) of incisor tooth, whisker and hair follicle epithelium rather than invagination. We show here that mutation of the Interferon regulatory factor (11) family, Irf6 also results in evagination of incisor epithelium. In common with Ikk alpha mutants, Irf6 mutant evagination occurs in a NF-kappa B-independent manner and shows the same molecular changes as those in Ikk alpha mutants. Irf6 thus also plays a critical role in regulating epithelial invagination. In addition, we also found that canonical Wnt signaling is upregulated in evaginated incisor epithelium of both Ikk alpha and Irf6 mutant embryos. (C) 2012 Elsevier Inc. All rights reserved.

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  • Sensory and Motor Responses of Normal Young Adults During Swallowing of Foods with Different Properties and Volumes Reviewed

    Atsuko Igarashi, Maiko Kawasaki, Shu-ichi Nomura, Yuji Sakai, Mayumi Ueno, Ichiro Ashida, Yozo Miyaoka

    DYSPHAGIA   25 ( 3 )   198 - 206   2010.9

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    We examined the influence of rheological/textural properties and volumes of test foods on the sensory and motor aspects of swallowing in healthy young adults. Three test foods differing in thickening agent concentration (0.0, 1.5, and 3.0%) were prepared and delivered in different volumes (similar to 3, similar to 5, and similar to 7 ml) to subjects seated on a chair. Viscosity analyses of the 1.5 and 3.0% test foods revealed that they behaved as non-Newtonian fluids and were thixotropic. The 1.5% test food differed from the 3.0% test food in its textural properties (hardness, cohesiveness, and adhesiveness). As determined by a linear model equation method, the thickening agent concentration affected the scores of all six sensory evaluation questions that were answered by the subjects, which suggests that the concentration affected the food properties being evaluated. Consistent with previous reports, thickening agent concentration and test food volume also affected some durational parameters of laryngeal (recorded by a piezoelectric sensor) and suprahyoid muscle (recorded on an electromyogram) motor activity. However, thickening agent concentration and test food volume did not affect the single amplitude parameter of the electromyogram that was measured. The thixotropic property of foods can affect the motor aspect of oropharyngeal swallowing as well as the sensory aspect.

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  • Effects of Hardness, Taste and Amount of Low-Gel-Strength Ager on Swallowing in Young and Elderly Subjects. Reviewed

    Sakai Y, Sugita K, Kawasaki M, Nomura S, Igarashi A

    J. Home Econ Jpn.   60 ( 2 )   133 - 138   2009

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    Low-gel-strength agar (LGSA), which has recently been developed as a material close to gelatin in its physical properties, was used in this study to prepare samples for evaluating the factors facilitating swallowing. The subjects were assigned to two groups of 20 healthy young persons (22.1±1.7 years) and 12 healthy elderly persons (72.2±3.3 years). The physical properties of each sample were measured with a creep meter. The hardness of the tasty samples [sweet (saccharose), salty (NaCl), and sour (citric acid)] was a little higher than the samples without taste. The sensory evaluation at swallowing was assessed in respect of the LGSA concentration (hardness), taste and amount of the sample. The healthy young subjects reported that a 1.1% LGSA concentration, sweet taste, and 5.0g amount, produced a sample that was pleasant in the oral cavity, facilitating swallowing. The healthy elderly subjects evaluated only a sweet taste, as facilitating swallowing. The results of the sensory evaluation and physical properties suggest that relative hardness (a 1.1% concentration of LGSA), sweet taste, and 5g amount resulted in samples that were pleasant in the oral cavity, thus facilitating swallowing.

    DOI: 10.11428/jhej.60.133

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  • Innovative Research on Biosis-Abiosis Intelligent Interface

    Kawasaki M, Kawasaki K, Blackburn J, Ohazama A, Sasaki K, Suzuki O, Takahashi N(Molecular mechanisms regulating tooth number)

    Springer Singapore  2016 

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  • Reptinは胎仔上皮におけるDNA損傷応答を介して器官形成を制御する

    目黒 史也, 柿原 嘉人, 川崎 真依子, 川崎 勝盛, 丹原 惇, トゥラカナン・スッパラック, 工藤 武久, 山田 茜, 前田 健康, 多部田 康一, 佐伯 万騎男, 大峡 淳

    新潟歯学会雑誌   50 ( 2 )   115 - 116   2020.12

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  • 下顎発生における一次繊毛の機能について

    北村厚, 北村厚, 川崎勝盛, 川崎真依子, 児玉泰光, 前田健康, 高木律男, 大峡淳

    新潟歯学会雑誌   47 ( 2 )   121‐122 - 122   2017.12

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  • 間葉のMicroRNAは,眼瞼発生を制御する

    永井孝宏, 永井孝宏, 渡部桃子, 渡部桃子, 川崎真依子, 川崎勝盛, 川崎勝盛, 北村厚, 北村厚, 児玉泰光, 高木律男, 前田健康, 大峡淳

    新潟歯学会雑誌   46 ( 2 )   114‐115 - 115   2016.12

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  • Ofd1は口蓋突起の下方伸長に必須である

    渡部桃子, 渡部桃子, 川崎勝盛, 川崎真依子, 永井孝宏, 永井孝宏, 北村厚, 北村厚, 児玉泰光, 前田健康, 高木律男, 大峡淳

    新潟歯学会雑誌   46 ( 2 )   108‐109 - 109   2016.12

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  • 口蓋発生におけるOfd1の役割について

    渡部桃子, 渡部桃子, 川崎真依子, 高木律男, 前田健康, 大峡淳

    Journal of Oral Biosciences Supplement (Web)   2015   ROMBUNNO.O2‐G2 (WEB ONLY)   2015

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  • Evaluation of implant prothesis by the mastication efficiency test and QOL survey

    KAWASAKI M, UOSHIMA K, YOSHIDA K, NAGASAWA M, MARCELO R, AL-AMIN B

    1 ( 118 )   181 - 181   2010.8

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  • The Establishment of Implant Occlusion Model Using Rat

    NAGASAWA M, UOSHIMA K, YOSHIDA K, KAWASAKI M, ROSALES M, AL-AMIN B

    1 ( 118 )   192 - 192   2010.8

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  • ラット咬合モデルを用いた咬合力に対するインプラント周囲骨変化の組織学的観察

    長澤麻沙子, 高野遼平, 吉田恵子, 川崎真依子, 前田健康, 魚島勝美

    日本補綴歯科学会学術大会プログラム・抄録集   119th   106   2010.5

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  • 咀嚼能率検査とQOLアンケートによる固定性インプラント義歯と部分床義歯の比較

    川崎真依子, 吉田恵子, 加来賢, 秋葉陽介, 長澤麻沙子, 藤井規孝, ROSALES M J.M., AL-AMIN MD.B, RASHID MD.M, 魚島勝美

    日本補綴歯科学会学術大会プログラム・抄録集   119th   2010

  • 間葉系幹細胞の骨形成系細胞誘導におけるヒストン脱アセチル化阻害剤の影響について

    秋葉陽介, アルアミン ブイアン, 川崎真依子, 加来賢, 加来賢, 魚島勝美, 魚島勝美

    日本補綴歯科学会学術大会プログラム・抄録集   119th   2010

  • コラーゲン修飾酵素の特異的発現が機械的刺激による歯根膜組織の安定化を制御する

    加来賢, 加来賢, 川崎真依子, 秋葉陽介, ROSALES M, RASHID M, 魚島勝美, 魚島勝美

    日本補綴歯科学会学術大会プログラム・抄録集   119th   2010

  • インプラント咬合動物実験モデルにおける骨の組織学的観察

    長澤麻沙子, 加来賢, 秋葉陽介, 吉田恵子, 川崎真依子, ROSALES Marcelo, AL‐AMIN Buiyan, 魚島勝美, 前田健康

    J Oral Biosci   51 ( Suppl. )   101 - 101   2009.8

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  • 新潟大学医歯学総合病院顎関節治療部における高齢者顎関節症患者に関する臨床統計的検討

    安島 久雄, 高木 律男, 荒井 良明, 池田 順行, 嵐山 貴徳, 庭野 将広, 西山 秀昌, 福井 忠雄, 高田 佳之, 桜井 直樹, 川崎 真依子

    日本顎関節学会雑誌   21 ( Suppl. )   93 - 93   2009.7

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Presentations

  • The role of the Ift88 in amelogenesis.

    Kudo T, Kawasaki M, Kawasaki K, Saito I, Ohazama A

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    Presentation type:Oral presentation (general)  

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  • MicroRNAs are involved in midfacial development

    Trakanant S, Kawasaki M, Kawasaki K, Saito I, Ohazama A

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    Event date: 2019.11

    Presentation type:Oral presentation (general)  

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  • Reptin regulates tooth development via suppressing apoptosis in tooth epithelium.

    Meguro F, Kakihara Y, Kawasaki K, Kawasaki M, Maeda T, Tabeta K, Saeki M, Ohazama A

    Tooth Morphogenesis & Differentiation  2019.1 

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    Event date: 2019.9

    Language:English   Presentation type:Oral presentation (general)  

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  • Overexpression of Ikkβ impairs enamel formation

    Yamada A, Meguro F, Kawasaki K, Kawasaki M, Ramírez A, Miake Y, Sharpe PT, Takagi R, Ohazama A

    Tooth Morphogenesis & Differentiation 

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    Event date: 2019.9

    Language:English   Presentation type:Oral presentation (general)  

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  • Bmp signaling regulates cusp formation Invited

    Meguro F, Kakihara Y, Kawasaki M, Kawasaki M, Maeda T, Tabeta K, Saeki M, Ohazama A

    International Niigata-Taiwan Universitues collaborative dental research symposium  2019.3 

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    Event date: 2019.3

    Language:English   Presentation type:Oral presentation (general)  

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  • Mesenchymal microRNAs regulate the development of the first branchial arch.

    Trakanant S, Kawasaki M, Kawasaki K, Saito I, Maeda T, Ohazama A

    International Niigata-Taiwan Universitues collaborative dental research symposium 

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    Event date: 2019.3

    Language:English   Presentation type:Oral presentation (general)  

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  • The role of primary cilia in ossicles development.

    Kawasaki K, Kawasaki M, Maeda M, Ohazama A

    International Niigata-Taiwan Universitues collaborative dental research symposium 

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    Event date: 2019.3

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  • The role of microRNAs in murine mandibular development

    Trakanant S, Kawasaki M, Kawasaki K, Saito I, Ohazama A

    2019.11 

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    Event date: 2019

    Presentation type:Oral presentation (general)  

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  • Reptinは胎仔上皮における DNA 損傷応答を介して器 官形成を制御する.

    目黒史也, 柿原嘉人, 川崎真依子, 川崎勝盛, 丹原, 淳、トゥラカナン スッパラック, 工藤武久, 山田 茜, 前田健康, 多部田康一, 佐伯万騎男, 大峽淳

    令和2年度新潟歯学会総会およ び第1回例会  2020.11 

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  • エナメル形成における Ift88 の機能解析

    工藤武久, 丹原惇, 川崎真依子, 川崎勝盛, Supaluk Trakanant, 目黒史也, 山田茜, 前田健, 康, 齋藤功, 大峡淳

    令和2年度新潟歯学会総会およ び第1回例会  2020.11 

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  • エナメル形成における NF-κB の機能解析

    山田 茜, 川﨑真依子, 川﨑勝盛, 工藤武久, 目黒, 史也, 高木律男, 大峡 淳

    令和2年度新潟歯学会総会およ び第1回例会  2020.7 

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  • マウス口蓋皺 壁発生における一次線毛の役割

    中庭まゆこ, 川崎真依子, 川崎勝盛, 目黒史也, 山田 茜, 前田健康, 大峡 淳

    令和元年度新潟歯学会第 2回例会  2019.11 

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  • The Role of Primary Cilia in Mandibular Development. International conference

    Kawasaki M, Kawasaki K, Ohazama A

    International Niigata-Taiwan Universitues collaborative dental research symposium  2019 

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  • The Role of Primary Cilia in Craniofacial Development. International conference

    Maiko Kawasaki

    International Collaborative Symposium on Development of Human Resources in Practical Oral Health and treatment  2017 

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  • The Role of Primary Cilia in Tongue Development. International conference

    Kawasaki M, Kawasaki K, Ohazama A

    Niigata-Taiwan University Dental Research Symposium  2017 

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  • The Role of Primary Cilia in Tooth Development. International conference

    Kawasaki M, Ohazama A

    InternationalSymposium on Oral Health Education and Research  2011 

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  • Comparison between implant prosthesis and removable partial denture by the mastication efficiency test and QOL survey

    Kawasaki M, Yoshida K, Kaku M, Akiba Y, Nagasawa M, Fujii N, J.M.Rosales Rocabado, Al-amin MD.Bhuiyan, Rashid MD Mamunur, Uoshima K

    Scientific Meeting of Japan Prosthodontic Society  2010 

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  • Evaluation of implant prothesis by the mastication efficiency test and QOL survey.

    Kawasaki M, Uoshima K, Yoshida K, Nagasawa M, J.M. ROSALES, Al-amin B

    Scientific Meeting of Japan Prosthodontic Society  2009 

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Awards

  • デンツプライ賞

    2010.5   日本補綴歯科学会   咀嚼能率検査とQOLアンケートによる固定性インプラント義歯と部分床義歯の比較

    川崎真依子

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Research Projects

  • Investigation for X-inactivation in palate formation

    Grant number:23K18354

    2023.6 - 2025.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Challenging Research (Exploratory)

    Awarding organization:Japan Society for the Promotion of Science

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    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

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  • 歯の形成におけるDNA損傷/修復のメカニズムの解明

    Grant number:23K09434

    2023.4 - 2026.3

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    川崎 真依子, 大峡 淳, 川崎 勝盛

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    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

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  • Mechanisms of the formation and maintenance of successional dental lamina

    Grant number:21K19591

    2021.7 - 2023.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Challenging Research (Exploratory)

    Awarding organization:Japan Society for the Promotion of Science

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    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

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  • Studying for mechanism of senescence activity in skin appendage development

    Grant number:21H03122

    2021.4 - 2024.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

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    Grant amount:\17550000 ( Direct Cost: \13500000 、 Indirect Cost:\4050000 )

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  • 顎顔面の発生過程における一次繊毛の機能解明:シグナル経路のクロストークの観点から

    Grant number:20K10092

    2020.4 - 2023.3

    System name:科学研究費助成事業 基盤研究(C)

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    川崎 真依子, 前田 健康, 大峡 淳, 川崎 勝盛

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    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    一次繊毛 primary ciliaは発生過程に関わる主要なシグナルの制御に関わり、その機能不全は顎顔面形成異常を伴う繊毛病を引き起こす。しかし、顎顔面形成における一次繊毛内での各種シグナル間の相互作用や、複数シグナルによる器官形成制御については未解明のままである。その原因の1つとして複数のシグナルを同時にターゲットとする研究がなされていないことがあげられる。本研究課題は、SHHとWNTシグナル経路を同時に変化させることによって引き起こる形態学的、分子的手法変化を解析することにより、顎顔面発生における一次繊毛の複数シグナル制御の機能を明らかにすることである。今年度は、SHHシグナルを間葉の組織特異的に欠損させ、WNTシグナルを間葉の組織特異的に過剰発現させたマウスの作成とその表現型の解析を行った。SHHシグナルを間葉の組織特異的に欠損させると同時にWNTシグナルを間葉の組織特異的に過剰発現させたマウスは、SHHシグナルを間葉の組織特異的に欠損させたマウスと一部異なる顎顔面領域の異常を示した。顔面の幅径の増加、および歯の形成遅延、眼瞼の形成異常などが挙げられ、これは、WNTシグナルが間葉で過剰発現していることが影響している可能性が示唆された。

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  • Trail toward development of new treatment for ameloblastoma by intentional cell differentiation

    Grant number:18K19639

    2018.6 - 2021.3

    System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Research category:Grant-in-Aid for Challenging Research (Exploratory)

    Awarding organization:Japan Society for the Promotion of Science

    MAEDA TAKEYASU

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    Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )

    At post-maturation stage in normal tooth development, ameloblasts differentiate into reduced enamel epithelial cells which exhibit low biological activity. These reduced enamel epithelial cells are fallen out during tooth eruption. It is believed that ameloblastoma is caused by tumorigenesis of enamel forming cells. It is possible that ameloblastoma become harmless when ameloblastoma differentiate into reduced enamel epithelial cells. However, the molecular mechanisms inducing reduced enamel epithelial cells are remained unclear. We found that reduced enamel epithelium decreased their biological activity by activating senescence.

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  • analysis of the comprehensive molecular mechanism of temporomandibular joint formation

    Grant number:18K09762

    2018.4 - 2021.3

    System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    kawasaki katsushige

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    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    Abnormalities of temporomandibular joint are seldom occurred in mice with targeted gene mutation. Therefore, the molecular mechanisms of temporomandibular joint formation remain unclear. We generated mice with mesenchymal deletions of the ciliary protein, Ift88 (Ift88fl/fl;Wnt1Cre), and found aberrant temporomandibular joint. The abnormal temporomandibular joint was found to be caused by aberrant mandibular formation. In Ift88fl/fl;Wnt1Cre mice, downregulation of Hh signaling led to the abnormal mandibular formation, which induced aberrant temporomandibular joint formation.

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  • 顎関節形成の包括的分子機構の解明

    2018 - 2020

    System name:基盤研究(C)

    Awarding organization:日本学術振興会

    川崎 勝盛

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    Grant type:Competitive

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  • 意図的細胞誘導による新規エナメル上皮腫治療法開発に向けた試み

    2018 - 2020

    System name:挑戦的研究(萌芽)

    Awarding organization:日本学術振興会

    前田健康

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    Grant type:Competitive

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  • 口蓋突起誘導メカニズムの解明

    2017 - 2019

    System name:基盤(C)

    Awarding organization:日本学術振興会

    川崎真依子

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  • 分化機構解明による幹細胞の意図的誘導法の開発

    2017 - 2019

    System name:基盤(A)

    Awarding organization:日本学術振興会

    大峡 淳

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    Grant type:Competitive

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  • 「生体完結型再生療法」開発への挑戦

    2017 - 2019

    System name:挑戦的研究(開拓)

    Awarding organization:日本学術振興会

    大峡 淳

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    Grant type:Competitive

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  • 歯の再生療法に向けた幹細胞分化制御機構の解明~毛との相同性、異同性に着目して~

    2016 - 2018

    System name:基盤(B)

    Awarding organization:日本学術振興会

    前田健康

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  • 遺伝子改変マウスを用いた歯誘導メカニズムの網羅的解析

    2016 - 2017

    System name:基盤(B)

    Awarding organization:日本学術振興会

    原田史子

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    Grant type:Competitive

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  • エピジェネティックスから探る口唇・口蓋の発生分子機構

    2013 - 2015

    System name:若手研究(B)

    Awarding organization:日本学術振興会

    川崎真依子

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  • 抗アポトーシスタンパクHSP27の細胞内導入法を用いた効果的な骨造成法の開発

    2011 - 2013

    System name:若手(B)

    Awarding organization:日本学術振興会

    川崎真依子

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    Authorship:Principal investigator  Grant type:Competitive

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  • 抗アポトーシスタンパクHSP27の細胞内導入法を用いた効果的な骨造成法の開発

    2010 - 2011

    System name:研究活動スタート支援

    Awarding organization:日本学術振興会

    川崎真依子

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Teaching Experience (researchmap)

Teaching Experience

  • 顎口腔解剖学演習IA

    2021
    Institution name:新潟大学

  • 基礎歯学コースワーク(ベーシック形態解析コースII)

    2021
    Institution name:新潟大学

  • 顎口腔解剖学演習IB

    2021
    Institution name:新潟大学

  • 顎口腔解剖学演習IIB

    2021
    Institution name:新潟大学

  • 顎口腔解剖学演習IIA

    2021
    Institution name:新潟大学

  • 基礎歯学コースワーク(ベーシック形態解析コースI)

    2021
    Institution name:新潟大学

  • 人体のしくみ

    2019
    Institution name:新潟大学

  • 歯学研究演習

    2017
    Institution name:新潟大学

  • 組織学各論

    2017
    Institution name:新潟大学

  • 口腔組織発生学

    2017
    Institution name:新潟大学

  • 組織学総論

    2017
    Institution name:新潟大学

  • 人体発生学

    2017
    Institution name:新潟大学

  • 早期臨床実習Ⅱ

    2016
    Institution name:新潟大学

  • 選択実習Ⅱ

    2010
    Institution name:新潟大学

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