2021/10/20 更新

写真a

カワサキ マイコ
川﨑 真依子
KAWASAKI Maiko
所属
教育研究院 医歯学系 歯学系列 准教授
医歯学総合研究科 口腔生命科学専攻 摂食環境制御学 准教授
職名
准教授
外部リンク

学位

  • 博士(歯学) ( 2010年3月   新潟大学 )

研究分野

  • ライフサイエンス / 外科系歯学

  • ライフサイエンス / 成長、発育系歯学

  • ライフサイエンス / 常態系口腔科学

経歴(researchmap)

  • 新潟大学 大学院医歯学総合研究科   Graduate School of Medical and Dental Sciences   准教授

    2019年4月 - 現在

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  • King’s College London   Craniofacial Development and Stem Cell Biology   Research Fellow

    2011年1月 - 2013年5月

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  • 新潟大学 大学院医歯学総合研究科   Graduate School of Medical and Dental Sciences   助教

    2010年4月 - 2019年3月

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経歴

  • 新潟大学   医歯学総合研究科 口腔生命科学専攻 摂食環境制御学   准教授

    2019年4月 - 現在

  • 新潟大学   医歯学総合研究科 口腔生命科学専攻 摂食環境制御学   助教

    2016年4月 - 2019年3月

  • 新潟大学   医歯学総合研究科 口腔生命科学専攻   助教

    2012年11月 - 2016年3月

  • 新潟大学   医歯学総合病院 摂食機能・補綴系歯科 冠・ブリッジ診療科   助教

    2012年11月 - 2016年3月

  • 新潟大学   医歯学総合病院 予防・保存系歯科   助教

    2010年4月 - 2012年11月

 

論文

  • Expression of R-spondins/Lgrs in development of movable craniofacial organs. 査読 国際誌

    Jun Nihara, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Fumiya Meguro, Takehisa Kudo, Supaluk Trakanant, Takahiro Nagai, Isao Saito, Takeyasu Maeda, Atsushi Ohazama

    Gene expression patterns : GEP   41   119195 - 119195   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Wnt signaling plays a critical role in the development of many organs, including the major movable craniofacial organs tongue, lip, and eyelid. Four members of the R-spondin family (Rspo1-4) bind to Lgr4/5/6 to regulate the activation of Wnt signaling. However, it is not fully understood how Rspos/Lgrs regulate Wnt signaling during the development of movable craniofacial organs. To address this question, we examined the expression of Rspos, Lgrs, and Axin2 (major mediator of canonical Wnt signaling) during tongue, lip, and eyelid development. The expression of Axin2, Rspos and Lgrs was observed in many similar regions, suggesting that Rspos likely activate canonical Wnt signaling through the Lgr-dependent pathway in these regions. Lgr expression was not detected in regions where Axin2 and Rspos were expressed, suggesting that Rspos might activate canonical Wnt signaling through the Lgr-independent pathway in these regions. In addition, the expression of Rspos and Lgrs were observed in some other regions where Axin2 was not expressed, suggesting the possibility that Rspos and/or Lgrs are involved in non-canonical Wnt signaling or the Wnt-independent pathway. Thus, we identified a dynamic spatiotemporal expression pattern of Rspos and Lgrs during the development of the eyelid, tongue, and lip.

    DOI: 10.1016/j.gep.2021.119195

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  • MicroRNAs regulate distal region of mandibular development through Hh signaling. 査読 国際誌

    Supaluk Trakanant, Jun Nihara, Takahiro Nagai, Maiko Kawasaki, Katsushige Kawasaki, Yoko Ishida, Fumiya Meguro, Takehisa Kudo, Akane Yamada, Takeyasu Maeda, Isao Saito, Atsushi Ohazama

    Journal of anatomy   238 ( 3 )   711 - 719   2021年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Mandibular anomalies are often seen in various congenital diseases, indicating that mandibular development is under strict molecular control. Therefore, it is crucial to understand the molecular mechanisms involved in mandibular development. MicroRNAs (miRNAs) are noncoding small single-stranded RNAs that play a critical role in regulating the level of gene expression. We found that the mesenchymal conditional deletion of miRNAs arising from a lack of Dicer (an essential molecule for miRNA processing, Dicerfl/fl ;Wnt1Cre), led to an abnormal groove formation at the distal end of developing mandibles. At E10.5, when the region forms, inhibitors of Hh signaling, Ptch1 and Hhip1 showed increased expression at the region in Dicer mutant mandibles, while Gli1 (a major mediator of Hh signaling) was significantly downregulated in mutant mandibles. These suggest that Hh signaling was downregulated at the distal end of Dicer mutant mandibles by increased inhibitors. To understand whether the abnormal groove formation inDicer mutant mandibles was caused by the downregulation of Hh signaling, mice with a mesenchymal deletion of Hh signaling activity arising from a lack of Smo (an essential molecule for Hh signaling activation, Smofl/fl ;Wnt1Cre) were examined. Smofl/fl ;Wnt1Cre mice showed a similar phenotype in the distal region of their mandibles to those in Dicerfl/fl ;Wnt1Cre mice. We also found that approximately 400 miRNAs were expressed in wild-type mandibular mesenchymes at E10.5, and six microRNAs were identified as miRNAs with binding potential against both Ptch1 and Hhip1. Their expressions at the distal end of the mandible were confirmed by in situ hybridization. This indicates that microRNAs regulate the distal part of mandibular formation at an early stage of development by involving Hh signaling activity through controlling its inhibitor expression level.

    DOI: 10.1111/joa.13328

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  • Changes in signalling pathways in the palatal cleft in CL/Fr mice 査読

    Akane Yamada, Takahiro Nagai, Atsushi Kitamura, Maiko Kawasaki, Katsushige Kawasaki, Yasumitsu Kodama, Takeyasu Maeda, Atsushi Ohazama, Ritsuo Takagi

    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   32 ( 5 )   331 - 335   2020年9月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.ajoms.2019.12.001

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  • Overactivation of the NF-κB pathway impairs molar enamel formation. 査読 国際誌

    Akane Yamada, Maiko Kawasaki, Yasuo Miake, Yurie Yamada, James Blackburn, Kataushige Kawasaki, Supaluk Trakanant, Takahiro Nagai, Jun Nihara, Takehisa Kudo, Fumiya Meguro, Ruth Schmidt-Ullrich, Bigang Liu, Yinling Hu, Angustias Page, Ángel Ramírez, Paul T Sharpe, Takeyasu Maeda, Ritsuo Takagi, Atsushi Ohazama

    Oral diseases   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: Hypohidrotic ectodermal dysplasia (HED) is a hereditary disorder characterized by abnormal structures and functions of the ectoderm-derived organs, including teeth. HED patients exhibit a variety of dental symptoms, such as hypodontia. Although disruption of the EDA/EDAR/EDARADD/NF-κB pathway is known to be responsible for HED, it remains unclear whether this pathway is involved in the process of enamel formation. EXPERIMENTAL SUBJECTS AND METHODS: To address this question, we examined the mice overexpressing Ikkβ (an essential component required for the activation of NF-κB pathway) under the keratin 5 promoter (K5-Ikkβ). RESULTS: Upregulation of the NF-κB pathway was confirmed in the ameloblasts of K5-Ikkβ mice. Premature abrasion was observed in the molars of K5-Ikkβ mice, which was accompanied by less mineralized enamel. However, no significant changes were observed in the enamel thickness and the pattern of enamel rods in K5-Ikkβ mice. Klk4 expression was significantly upregulated in the ameloblasts of K5-Ikkβ mice at the maturation stage, and the expression of its substrate, amelogenin, was remarkably reduced. This suggests that abnormal enamel observed in K5-Ikkβ mice was likely due to the compromised degradation of enamel protein at the maturation stage. CONCLUSION: Therefore, we could conclude that the overactivation of the NF-κB pathway impairs the process of amelogenesis.

    DOI: 10.1111/odi.13384

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  • Molecular mechanisms in palatal rugae development. 査読 国際誌

    Supaluk Trakanant, Jun Nihara, Maiko Kawasaki, Fumiya Meguro, Akane Yamada, Katsushige Kawasaki, Isao Saito, Maeda Takeyasu, Atsushi Ohazama

    Journal of oral biosciences   62 ( 1 )   30 - 35   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Periodic patterning of iterative structures is diverse across the animal kingdom. Clarifying the molecular mechanisms involved in the formation of these structures helps to elucidate the genetic commonality of developmental processes, as organs with these structures are believed to share the same molecular mechanisms and fundamental processes. Palatal rugae are periodic corrugated structures on the hard palate and are conserved in all mammals. Although the numbers and patterns of the palatal rugae are species specific, they are consistent in each mammalian species, except humans. HIGHLIGHT: Palatal rugae development is thus under strict genetic control in most mammals and is an excellent model to investigate the genetic commonality of developmental processes to form periodic patterning. CONCLUSION: This review highlights the current understanding of the molecular mechanisms of palatal rugae development.

    DOI: 10.1016/j.job.2019.12.002

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  • Ift88 is involved in mandibular development. 査読 国際誌

    Atsushi Kitamura, Maiko Kawasaki, Katsushige Kawasaki, Fumiya Meguro, Akane Yamada, Takahiro Nagai, Yasumitsu Kodama, Supaluk Trakanant, Paul T Sharpe, Takeyasu Maeda, Ritsuo Takagi, Atsushi Ohazama

    Journal of anatomy   236 ( 2 )   317 - 324   2020年2月

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    記述言語:英語  

    The mandible is a crucial organ in both clinical and biological fields due to the high frequency of congenital anomalies and the significant morphological changes during evolution. Primary cilia play a critical role in many biological processes, including the determination of left/right axis patterning, the regulation of signaling pathways, and the formation of bone and cartilage. Perturbations in the function of primary cilia are known to cause a wide spectrum of human diseases: the ciliopathies. Craniofacial dysmorphologies, including mandibular deformity, are often seen in patients with ciliopathies. Mandibular development is characterized by chondrogenesis and osteogenesis; however, the role of primary cilia in mandibular development is not fully understood. To address this question, we generated mice with mesenchymal deletions of the ciliary protein, Ift88 (Ift88fl/fl ;Wnt1Cre). Ift88fl/fl ;Wnt1Cre mice showed ectopic mandibular bone formation, whereas Ift88 mutant mandible was slightly shortened. Meckel's cartilage was modestly expanded in Ift88fl/fl ;Wnt1Cre mice. The downregulation of Hh signaling was found in most of the mesenchyme of Ift88 mutant mandible. However, mice with a mesenchymal deletion of an essential molecule for Hh signaling activity, Smo (Smofl/fl ;Wnt1Cre), showed only ectopic mandibular formation, whereas Smo mutant mandible was significantly shortened. Ift88 is thus involved in chondrogenesis and osteogenesis during mandibular development, partially through regulating Sonic hedgehog (Shh) signaling.

    DOI: 10.1111/joa.13096

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  • Primary cilia in murine palatal rugae development. 査読 国際誌

    Mayuko Nakaniwa, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Fumiya Meguro, Maeda Takeyasu, Atsushi Ohazama

    Gene expression patterns : GEP   34   119062 - 119062   2019年12月

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    記述言語:英語  

    Periodic patterning of iterative structures is a fundamental process during embryonic development, since these structures are diverse across the animal kingdom. Therefore, elucidating the molecular mechanisms in the formation of these structures promotes understanding of the process of organogenesis. Periodically patterned ridges, palatal rugae (situated on the hard palate of mammals), are an excellent experimental model to clarify the molecular mechanisms involved in the formation of periodic patterning of iterative structures. Primary cilia are involved in many biological events, including the regulation of signaling pathways such as Shh and non-canonical Wnt signaling. However, the role of primary cilia in the development of palatal rugae remains unclear. We found that primary cilia were localized to the oral cavity side of the interplacode epithelium of the palatal rugae, whereas restricted localization of primary cilia could not be detected in other regions. Next, we generated mice with a placodal conditional deletion of the primary cilia protein Ift88, using ShhCre mice (Ift88 fl/fl;ShhCre). Highly disorganized palatal rugae were observed in Ift88 fl/fl;ShhCre mice. Furthermore, by comparative in situ hybridization analysis, many Shh and non-canonical Wnt signaling-related molecules showed spatiotemporal expression patterns during palatal rugae development, including restricted expression in the epithelium (placodes and interplacodes) and mesenchyme. Some of these expression were found to be altered in Ift88 fl/fl;ShhCre mice. Primary cilia is thus involved in development of palatal rugae.

    DOI: 10.1016/j.gep.2019.119062

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  • Bmp signaling in molar cusp formation. 査読 国際誌

    Fumiya Meguro, Thantrira Porntaveetus, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Yoshito Kakihara, Makio Saeki, Koichi Tabeta, John A Kessler, Takeyasu Maeda, Atsushi Ohazama

    Gene expression patterns : GEP   32   67 - 71   2019年6月

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    記述言語:英語  

    Tooth cusp is a crucial structure, since the shape of the molar tooth is determined by number, shape, and size of the cusp. Bone morphogenetic protein (Bmp) signaling is known to play a critical role in tooth development, including in initiation. However, it remains unclear whether Bmp signaling is also involved in cusp formation. To address this question, we examined cusp in two different transgenic mouse lines: mice with overexpression of Bmp4 (K14-Bmp4), and those with Bmp inhibitor, Noggin, (K14-Noggin) under keratin14 (K14) promoter. K14-Noggin mice demonstrated extra cusps, whereas reduced number of cusps was observed in K14-Bmp4 mice. To further understand how Bmps are expressed during cusp formation, we performed whole-mount in situ hybridisation analysis of three major Bmps (Bmp2, Bmp4, and Bmp7) in murine maxillary and mandibular molars from E14.5 to P3. The linear expressions of Bmp2 and Bmp4 were observed in both maxillary and mandibular molars at E14.5. The expression patterns of Bmp2 and Bmp4 became significantly different between the maxillary and mandibular molars at E16.5. At P3, all Bmps were expressed in all the cusp regions of the maxillary molar; however, the patterns differed. All Bmps thus exhibited dynamic temporo-spatial expression during the cusp formation. It could therefore be inferred that Bmp signaling is involved in regulating cusp formation.

    DOI: 10.1016/j.gep.2019.04.002

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  • Ift88 limits bone formation in maxillary process through suppressing apoptosis. 査読 国際誌

    Momoko Watanabe, Maiko Kawasaki, Katsushige Kawasaki, Atsushi Kitamura, Takahiro Nagai, Yasumitsu Kodama, Fumiya Meguro, Akane Yamada, Paul T Sharpe, Takeyasu Maeda, Ritsuo Takagi, Atsushi Ohazama

    Archives of oral biology   101   43 - 50   2019年5月

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    記述言語:英語  

    OBJECTIVE: The development of the maxillary bone is under strict molecular control because of its complicated structure. Primary cilia play a critical role in craniofacial development, since defects in primary cilia are known to cause congenital craniofacial dysmorphologies as a wide spectrum of human diseases: the ciliopathies. The primary cilia also are known to regulate bone formation. However, the role of the primary cilia in maxillary bone development is not fully understood. DESIGN: To address this question, we generated mice with a mesenchymal conditional deletion ofIft88 using the Wnt1Cre mice (Ift88fl/fl;Wnt1Cre). The gene Ift88 encodes a protein that is required for the function and formation of primary cilia. RESULTS: It has been shown thatIft88fl/fl;Wnt1Cre mice exhibit cleft palate. Here, we additionally observed excess bone formation in the Ift88 mutant maxillary process. We also found ectopic apoptosis in the Ift88 mutant maxillary process at an early stage of development. To investigate whether the ectopic apoptosis is related to the Ift88 mouse maxillary phenotypes, we generated Ift88fl/fl;Wnt1Cre;p53-/- mutants to reduce apoptosis. The Ift88fl/fl;Wnt1Cre;p53-/- mice showed no excess bone formation, suggesting that the cells evading apoptosis by the presence of Ift88 in wild-type mice limit bone formation in maxillary development. On the other hand, the palatal cleft was retained in the Ift88fl/fl;Wnt1Cre;p53-/- mice, indicating that the excess bone formation or abnormal apoptosis was independent of the cleft palate phenotype in Ift88 mutant mice. CONCLUSIONS: Ift88 limits bone formation in the maxillary process by suppressing apoptosis.

    DOI: 10.1016/j.archoralbio.2019.02.017

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  • MicroRNAs control eyelid development through regulating Wnt signaling. 査読 国際誌

    Takahiro Nagai, Supaluk Trakanant, Maiko Kawasaki, Katsushige Kawasaki, Yurie Yamada, Momoko Watanabe, James Blackburn, Yoko Otsuka-Tanaka, Mitsue Hishinuma, Atsushi Kitatmura, Fumiya Meguro, Akane Yamada, Yasumitsu Kodama, Takeyasu Maeda, Qiliang Zhou, Yasuo Saijo, Akihiro Yasue, Paul T Sharpe, Robert Hindges, Ritsuo Takagi, Atsushi Ohazama

    Developmental dynamics : an official publication of the American Association of Anatomists   248 ( 3 )   201 - 210   2019年3月

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    記述言語:英語  

    BACKGROUND: The timing, location, and level of gene expression are crucial for normal organ development, because morphogenesis requires strict genetic control. MicroRNAs (miRNAs) are noncoding small single-stranded RNAs that play a critical role in regulating gene expression level. Although miRNAs are known to be involved in many biological events, the role of miRNAs in organogenesis is not fully understood. Mammalian eyelids fuse and separate during development and growth. In mice, failure of this process results in the eye-open at birth (EOB) phenotype. RESULTS: It has been shown that conditional deletion of mesenchymal Dicer (an essential protein for miRNA processing; Dicer fl/fl ;Wnt1Cre) leads to the EOB phenotype with full penetrance. Here, we identified that the up-regulation of Wnt signaling resulted in the EOB phenotype in Dicer mutants. Down-regulation of Fgf signaling observed in Dicer mutants was caused by an inverse relationship between Fgf and Wnt signaling. Shh and Bmp signaling were down-regulated as the secondary effects in Dicer fl/fl ;Wnt1Cre mice. Wnt, Shh, and Fgf signaling were also found to mediate the epithelial-mesenchymal interactions in eyelid development. CONCLUSIONS: miRNAs control eyelid development through Wnt. Developmental Dynamics 248:201-210, 2019. © 2019 Wiley Periodicals, Inc.

    DOI: 10.1002/dvdy.10

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  • Lrp4/Wise regulates palatal rugae development through Turing-type reaction-diffusion mechanisms. 査読 国際誌

    Maiko Kawasaki, Katsushige Kawasaki, Fumiya Meguro, Akane Yamada, Ryuichi Ishikawa, Thantrira Porntaveetus, James Blackburn, Yoko Otsuka-Tanaka, Naoaki Saito, Masato S Ota, Paul T Sharpe, John A Kessler, Joachim Herz, Martyn T Cobourne, Takeyasu Maeda, Atsushi Ohazama

    PloS one   13 ( 9 )   e0204126   2018年

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    記述言語:英語  

    Periodic patterning of iterative structures is diverse across the animal kingdom. Clarifying the molecular mechanisms involved in the formation of these structure helps to elucidate the process of organogenesis. Turing-type reaction-diffusion mechanisms have been shown to play a critical role in regulating periodic patterning in organogenesis. Palatal rugae are periodically patterned ridges situated on the hard palate of mammals. We have previously shown that the palatal rugae develop by a Turing-type reaction-diffusion mechanism, which is reliant upon Shh (as an inhibitor) and Fgf (as an activator) signaling for appropriate organization of these structures. The disturbance of Shh and Fgf signaling lead to disorganized palatal rugae. However, the mechanism itself is not fully understood. Here we found that Lrp4 (transmembrane protein) was expressed in a complementary pattern to Wise (a secreted BMP antagonist and Wnt modulator) expression in palatal rugae development, representing Lrp4 expression in developing rugae and Wise in the inter-rugal epithelium. Highly disorganized palatal rugae was observed in both Wise and Lrp4 mutant mice, and these mutants also showed the downregulation of Shh signaling, which was accompanied with upregulation of Fgf signaling. Wise and Lrp4 are thus likely to control palatal rugae development by regulating reaction-diffusion mechanisms through Shh and Fgf signaling. We also found that Bmp and Wnt signaling were partially involved in this mechanism.

    DOI: 10.1371/journal.pone.0204126

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  • Expanding the Oro-Dental and Mutational Spectra of Kabuki Syndrome and Expression of KMT2D and KDM6A in Human Tooth Germs. 査読 国際誌

    Thantrira Porntaveetus, Mushriq F Abid, Thanakorn Theerapanon, Chalurmpon Srichomthong, Atsushi Ohazama, Katsushige Kawasaki, Maiko Kawasaki, Kanya Suphapeetiporn, Paul T Sharpe, Vorasuk Shotelersuk

    International journal of biological sciences   14 ( 4 )   381 - 389   2018年

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    記述言語:英語  

    Kabuki syndrome is a rare genetic disorder characterized by distinct dysmorphic facial features, intellectual disability, and multiple developmental abnormalities. Despite more than 350 documented cases, the oro-dental spectrum associated with kabuki syndrome and expression of KMT2D (histone-lysine N-methyltransferase 2D) or KDM6A (lysine-specific demethylase 6A) genes in tooth development have not been well defined. Here, we report seven unrelated Thai patients with Kabuki syndrome having congenital absence of teeth, malocclusion, high-arched palate, micrognathia, and deviated tooth shape and size. Exome sequencing successfully identified that six patients were heterozygous for mutations in KMT2D, and one in KDM6A. Six were novel mutations, of which five were in KMT2D and one in KDM6A. They were truncating mutations including four frameshift deletions and two nonsense mutations. The predicted non-functional KMT2D and KDM6A proteins are expected to cause disease by haploinsufficiency. Our study expands oro-dental, medical, and mutational spectra associated with Kabuki syndrome. We also demonstrate for the first time that KMT2D and KDM6A are expressed in the dental epithelium of human tooth germs.

    DOI: 10.7150/ijbs.23517

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  • Sox Genes Show Spatiotemporal Expression during Murine Tongue and Eyelid Development. 査読 国際誌

    Ryuichi Ishikawa, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Supaluk Trakanant, Fumiya Meguro, Atsushi Kitamura, Takehisa Kudo, Takeyasu Maeda, Atsushi Ohazama

    International journal of dentistry   2018   1601363 - 1601363   2018年

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    記述言語:英語  

    The tongue is a critical organ, involved in functions such as speaking, swallowing, mastication, and degustation. Although Sox genes are known to play critical roles in many biological processes, including organogenesis, the expression of the Sox family members during tongue development remains unclear. We therefore performed a comparative in situ hybridization analysis of 17 Sox genes (Sox1-14, 17, 18, and 21) during murine tongue development. Sox2, 4, 6, 8, 9, 10, 11, 12, and 21 were found to be expressed in the tongue epithelium, whereas Sox2, 4-6, 8-11, 13, and 21 showed expression in the mesenchyme of the developing tongue. Expression of Sox1, 4, 6, 8-12, and 21 were observed in the developing tongue muscle. Sox5 and 13 showed expression only at E12, while Sox1 expression was observed only on E18. Sox6, 8, 9, and 12 showed expression at several stages. Although the expression of Sox2, 4, 10, 11, and 21 was detected during all the four stages of tongue development, their expression patterns differed among the stages. We thus identified a dynamic spatiotemporal expression pattern of the Sox genes during murine tongue development. To understand whether Sox genes are involved in the development of other craniofacial organs through similar roles to those in tongue development, we also examined the expression of Sox genes in eyelid primordia, which also contain epithelium, mesenchyme, and muscle. However, expression patterns and timing of Sox genes differed between tongue and eyelid development. Sox genes are thus related to organogenesis through different functions in each craniofacial organ.

    DOI: 10.1155/2018/1601363

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  • Regional regulation of Filiform tongue papillae development by Ikkα/Irf6. 査読

    Kawasaki M, Kawasaki K, Oommen S, Blackburn J, Watanabe M, Nagai T, Kitamura A, Maeda T, Liu B, Schmidt-Ullrich R, Akiyama T, Inoue J, Hammond NL, Sharpe PT, Ohazama A

    Developmental dynamics : an official publication of the American Association of Anatomists   245 ( 9 )   937 - 946   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/DVDY.24427

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  • Spatio-temporal expression of Sox genes in murine palatogenesis 査読

    Momoko Watanabe, Katsushige Kawasaki, Maiko Kawasaki, Thantrira Portaveetus, Shelly Oommen, James Blackburn, Takahiro Nagai, Atsushi Kitamura, Atsushi Nishikawa, Yasumitsu Kodama, Ritsuo Takagi, Takeyasu Maeda, Paul T. Sharpe, Atsushi Ohazama

    GENE EXPRESSION PATTERNS   21 ( 2 )   111 - 118   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Members of the Sox gene family play critical roles in many biological processes including organogenesis. We carried out comparative in situ hybridisation analysis of seventeen Sox genes (Sox1-14, 17, 18 and 21) during murine palatogenesis from initiation to fusion of the palatal shelves above the dorsal side of the tongue. At palatal shelf initiation (E12.5), the localized expression of six Sox genes (Sox2, 5, 6, 9,12 and 13) was observed in the shelves, whereas Sox4 and Sox11 showed ubiquitious expression. During the down growth of palatal shelves (E13.5), Sox4, Sox5, and Sox9 exhibited restricted expression to the interior side of the palatal shelves facing the tongue. Following elevation of the palatal shelves (E14.5), Sox2, Sox11 and Sox21 expression was present in the midline epithelial seam. We thus identify dynamic spatio-temporal expression of Sox gene family during the process of palatogenesis. (C) 2016 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.gep.2016.05.002

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  • Excess NF-κB induces ectopic odontogenesis in embryonic incisor epithelium. 査読

    Blackburn J, Kawasaki K, Porntaveetus T, Kawasaki M, Otsuka-Tanaka Y, Miake Y, Ota MS, Watanabe M, Hishinuma M, Nomoto T, Oommen S, Ghafoor S, Harada F, Nozawa-Inoue K, Maeda T, Peterková R, Lesot H, Inoue J, Akiyama T, Schmidt-Ullrich R, Liu B, Hu Y, Page A, Ramírez Á, Sharpe PT, Ohazama A

    J Dent Res.   94 ( 1 )   121 - 128   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/0022034514556707

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  • Expression of Sox genes in tooth development 査読

    Katsushige Kawasaki, Maiko Kawasaki, Momoko Watanabe, Erik Idrus, Takahiro Nagai, Shelly Oommen, Takeyasu Maeda, Nobuko Hagiwara, Jianwen Que, Paul T. Sharpe, Atsushi Ohazama

    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY   59 ( 10-12 )   471 - 478   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:UNIV BASQUE COUNTRY UPV-EHU PRESS  

    Members of the Sox gene family play roles in many biological processes including organogenesis. We carried out comparative in situ hybridization analysis of seventeen sox genes (Sox 1-14, 17, 18, 21) during murine odontogenesis from the epithelial thickening to the cytodifferentiation stages. Localized expression of five Sox genes (Sox6, 9, 13, 14 and 21) was observed in tooth bud epithelium. Sox13 showed restricted expression in the primary enamel knots. At the early bell stage, three Sox genes (Sox8, 11, 17 and 21) were expressed in pre-ameloblasts, whereas two others (Sox5 and 18) showed expression in odontoblasts. Sox genes thus showed a dynamic spatio-temporal expression during tooth development.

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  • Analysis of Oral Moisturizing Effects of Trehalose Tablets. 査読

    Kawasaki M, Suzuki A, Sakai Y, Sohda M, Igarashi A, Watanabe T

    NIIGATA DENTAL JOURNAL   45 ( 2 )   13 - 20   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • R-spondins/Lgrs expression in tooth development 査読

    Maiko Kawasaki, Thantrira Porntaveetus, Katsushige Kawasaki, Shelly Oommen, Yoko Otsuka-Tanaka, Mitsue Hishinuma, Takato Nomoto, Takeyasu Maeda, Keiyo Takubo, Toshio Suda, Paul T. Sharpe, Atsushi Ohazama

    DEVELOPMENTAL DYNAMICS   243 ( 6 )   844 - 851   2014年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Background: Tooth development is highly regulated in mammals and it is regulated by networks of signaling pathways (e. g. Tnf, Wnt, Shh, Fgf and Bmp) whose activities are controlled by the balance between ligands, activators, inhibitors and receptors. The members of the R-spondin family are known as activators of Wnt signaling, and Lgr4, Lgr5, and Lgr6 have been identified as receptors for R-spondins. The role of R-spondin/Lgr signaling in tooth development, however, remains unclear. Results: We first carried out comparative in situ hybridization analysis of R-spondins and Lgrs, and identified their dynamic spatio-temporal expression in murine odontogenesis. R-spondin2 expression was found both in tooth germs and the tooth-less region, the diastema. We further examined tooth development in R-spondin2 mutant mice, and although molars and incisors exhibited no significant abnormalities, supernumerary teeth were observed in the diastema. Conclusions: R-spondin/Lgr signaling is thus involved in tooth development. Developmental Dynamics 243:844-851, 2014. (c) 2014 Wiley Periodicals, Inc.

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  • PKA regulatory subunit expression in tooth development 査読

    Silvia Ferreira de Sousa, Katsushige Kawasaki, Maiko Kawasaki, Ana Angelova Volponi, Ricardo Santiago Gomez, Carolina Cavalieri Gomes, Paul T. Sharpe, Atsushi Ohazama

    GENE EXPRESSION PATTERNS   15 ( 1 )   46 - 51   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Protein kinase A (PK A) plays critical roles in many biological processes including cell proliferation, cell differentiation, cellular metabolism and gene regulation. Mutation in PKA regulatory subunit, PRKAR1A has previously been identified in odontogenic myxomas, but it is unclear whether PKA is involved in tooth development. The aim of the present study was to assess the expression of alpha isoforms of PICA regulatory subunit (Prkar1a and Prkar2a) in mouse and human odontogenesis by in situ hybridization. PRKAR1A and PRKAR2A mRNA transcription was further confirmed in a human deciduous germ by qRT-PCR. Mouse Prkar1a and human PRKAR2A exhibited a dynamic spatio-temporal expression in tooth development, whereas neither human PRKAR1A nor mouse Prkar2a showed their expression in odontogenesis. These isoforms thus showed different expression pattern between human and mouse tooth germs. (C) 2014 Elsevier B.V. All rights reserved.

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  • Multiple postnatal craniofacial anomalies are characterized by conditional loss of polycystic kidney disease 2 (Pkd2) 査読

    Roman H. Khonsari, Atsushi Ohazama, Ramin Raouf, Maiko Kawasaki, Katsushige Kawasaki, Thantrira Porntaveetus, Sarah Ghafoor, Peter Hammond, Michael Suttie, Guillaume A. Odri, Richard N. Sandford, John N. Wood, Paul T. Sharpe

    HUMAN MOLECULAR GENETICS   22 ( 9 )   1873 - 1885   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Polycystin 2 (Pkd2), which belongs to the transient receptor potential family, plays a critical role in development. Pkd2 is mainly localized in the primary cilia, which also function as mechanoreceptors in many cells that influence multiple biological processes including Ca-2 influx, chemical activity and signalling pathways. Mutations in many cilia proteins result in craniofacial abnormalities. Orofacial tissues constantly receive mechanical forces and are known to develop and grow through intricate signalling pathways. Here we investigate the role of Pkd2, whose role remains unclear in craniofacial development and growth. In order to determine the role of Pkd2 in craniofacial development, we located expression in craniofacial tissues and analysed mice with conditional deletion of Pkd2 in neural crest-derived cells, using Wnt1Cre mice. Pkd2 mutants showed many signs of mechanical trauma such as fractured molar roots, distorted incisors, alveolar bone loss and compressed temporomandibular joints, in addition to abnormal skull shapes. Significantly, mutants showed no indication of any of these phenotypes at embryonic stages when heads perceive no significant mechanical stress in utero. The results suggest that Pkd2 is likely to play a critical role in craniofacial growth as a mechanoreceptor. Pkd2 is also identified as one of the genes responsible for autosomal dominant polycystic kidney disease (ADPKD). Since facial anomalies have never been identified in ADPKD patients, we carried out three-dimensional photography of patient faces and analysed these using dense surface modelling. This analysis revealed specific characteristics of ADPKD patient faces, some of which correlated with those of the mutant mice.

    DOI: 10.1093/hmg/ddt041

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  • Adult Human Gingival Epithelial Cells as a Source for Whole-tooth Bioengineering 査読

    A. Angelova Volponi, M. Kawasaki, P. T. Sharpe

    JOURNAL OF DENTAL RESEARCH   92 ( 4 )   329 - 334   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    Teeth develop from interactions between embryonic oral epithelium and neural-crest-derived mesenchyme. These cells can be separated into single-cell populations and recombined to form normal teeth, providing a basis for bioengineering new teeth if suitable, non-embryonic cell sources can be identified. We show here that cells can be isolated from adult human gingival tissue that can be expanded in vitro and, when combined with mouse embryonic tooth mesenchyme cells, form teeth. Teeth with developing roots can be produced from this cell combination following transplantation into renal capsules. These bioengineered teeth contain dentin and enamel with ameloblast-like cells and rests of Malassez of human origin.

    DOI: 10.1177/0022034513481041

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  • The buccohypophyseal canal is an ancestral vertebrate trait maintained by modulation in sonic hedgehog signaling 査読

    Roman H. Khonsari, Maisa Seppala, Alan Pradel, Hugo Dutel, Gael Clement, Oleg Lebedev, Sarah Ghafoor, Michaela Rothova, Abigael Tucker, John G. Maisey, Chen-Ming Fan, Maiko Kawasaki, Atsushi Ohazama, Paul Tafforeau, Brunella Franco, Jill Helms, Courtney J. Haycraft, Albert David, Philippe Janvier, Martyn T. Cobourne, Paul T. Sharpe

    BMC BIOLOGY   11   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: The pituitary gland is formed by the juxtaposition of two tissues: neuroectoderm arising from the basal diencephalon, and oral epithelium, which invaginates towards the central nervous system from the roof of the mouth. The oral invagination that reaches the brain from the mouth is referred to as Rathke's pouch, with the tip forming the adenohypophysis and the stalk disappearing after the earliest stages of development. In tetrapods, formation of the cranial base establishes a definitive barrier between the pituitary and oral cavity; however, numerous extinct and extant vertebrate species retain an open buccohypophyseal canal in adulthood, a vestige of the stalk of Rathke's pouch. Little is currently known about the formation and function of this structure. Here we have investigated molecular mechanisms driving the formation of the buccohypophyseal canal and their evolutionary significance.
    Results: We show that Rathke's pouch is located at a boundary region delineated by endoderm, neural crest-derived oral mesenchyme and the anterior limit of the notochord, using CD1, R26R-Sox17-Cre and R26R-Wnt1-Cre mouse lines. As revealed by synchrotron X-ray microtomography after iodine staining in mouse embryos, the pouch has a lobulated three-dimensional structure that embraces the descending diencephalon during pituitary formation. Polaris(fl/fl); Wnt1-Cre, Ofd1(-/-) and Kif3a(-/-) primary cilia mouse mutants have abnormal sonic hedgehog (Shh) signaling and all present with malformations of the anterior pituitary gland and midline structures of the anterior cranial base. Changes in the expressions of Shh downstream genes are confirmed in Gas1(-/-) mice. From an evolutionary perspective, persistence of the buccohypophyseal canal is a basal character for all vertebrates and its maintenance in several groups is related to a specific morphology of the midline that can be related to modulation in Shh signaling.
    Conclusion: These results provide insight into a poorly understood ancestral vertebrate structure. It appears that the opening of the buccohypophyseal canal depends upon Shh signaling and that modulation in this pathway most probably accounts for its persistence in phylogeny.

    DOI: 10.1186/1741-7007-11-27

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  • The buccohypophyseal canal is an ancestral vertebrate trait maintained by modulation in sonic hedgehog signaling. 査読

    Khonsari RH, Seppala M, Pradel A, Dutel H, Clément G, Lebedev O, Ghafoor S, Rothova M, Tucker A, Maisey JG, Fan CM, Kawasaki M, Ohazama A, Tafforeau P, Franco B, Helms J, Haycraft CJ, David A, Janvier P, Cobourne MT, Sharpe PT

    BMC biology   11   27   2013年3月

  • Oral Lining Mucosa Development Depends on Mesenchymal microRNAs 査読

    Otsuka-Tanaka Y, Oommen S, Kawasaki M, Kawasaki K, Imam N, Jalani-Ghazani F, Hindges R, Sharpe PT, Ohazama A

    J Dent Res.   92 ( (3) )   229 - 234   2012年12月

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  • Cytoplasmic Plaque Formation in Hemidesmosome Development Is Dependent on SoxF Transcription Factor Function 査読

    Shelly Oommen, Mathias Francois, Maiko Kawasaki, Melanie Murrell, Katsushige Kawasaki, Thantrira Porntaveetus, Sarah Ghafoor, Neville J. Young, Yoshimasa Okamatsu, John McGrath, Peter Koopman, Paul T. Sharpe, Atsushi Ohazama

    PLOS ONE   7 ( 9 )   e43857   2012年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Hemidesmosomes are composed of intricate networks of proteins, that are an essential attachment apparatus for the integrity of epithelial tissue. Disruption leads to blistering diseases such as epidermolysis bullosa. Members of the Sox gene family show dynamic and diverse expression patterns during development and mutation analyses in humans and mice provide evidence that they play a remarkable variety of roles in development and human disease. Previous studies have established that the mouse mutant ragged-opossum (Ra-op) expresses a dominant-negative form of the SOX18 transcription factor that interferes with the function of wild type SOX18 and of the related SOXF-subgroup proteins SOX7 and -17. Here we show that skin and oral mucosa in homozygous Ra-op mice display extensive detachment of epithelium from the underlying mesenchymal tissue, caused by tearing of epithelial cells just above the plasma membrane due to hemidesmosome disruption. In addition, several hemidesmosome proteins expression were found to be dysregulated in the Ra-op mice. Our data suggest that SOXF transcription factors play a role in regulating formation of cytoplasmic plaque protein assembly, and that disrupted SOXF function results in epidermolysis bullosa-like skin phenotypes.

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  • Distinct roles of MicroRNAs in epithelium and mesenchyme during tooth development 査読

    Shelly Oommen, Yoko Otsuka-Tanaka, Najam Imam, Maiko Kawasaki, Katsushige Kawasaki, Farnoosh Jalani-Ghazani, Angela Anderegg, Rajeshwar Awatramani, Robert Hindges, Paul T. Sharpe, Atsushi Ohazama

    DEVELOPMENTAL DYNAMICS   241 ( 9 )   1465 - 1472   2012年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Background: Tooth development is known to be mediated by the cross-talk between signaling pathways, including Shh, Fgf, Bmp, and Wnt. MicroRNAs (miRNAs) are 19- to 25-nt noncoding small single-stranded RNAs that negatively regulate gene expression by binding target mRNAs, which is believed to be important for the fine-tuning signaling pathways in development. To investigate the role of miRNAs in tooth development, we examined mice with either mesenchymal (Wnt1Cre/Dicerfl/fl) or epithelial (ShhCre/Dicerfl/fl) conditional deletion of Dicer, which is essential for miRNA processing. Results: By using a CD1 genetic background for Wnt1Cre/Dicerfl/fl, we were able to examine tooth development, because the mutants retained mandible and maxilla primordia. Wnt1Cre/Dicerfl/fl mice showed an arrest or absence of teeth development, which varied in frequency between incisors and molars. Extra incisor tooth formation was found in ShhCre/Dicerfl/fl mice, whereas molars showed no significant anomalies. Microarray and in situ hybridization analysis identified several miRNAs that showed differential expression between incisors and molars. Conclusion: In tooth development, miRNAs thus play different roles in epithelium and mesenchyme, and in incisors and molars. Developmental Dynamics 241:1465-1472, 2012. (c) 2012 Wiley Periodicals, Inc.

    DOI: 10.1002/dvdy.23828

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  • Evaluation of newly developed devices for denture placement and removal in the dependent elderly 査読

    Maiko Kawasaki, Shuichi Nomura, Naoto Okada, Akiko Nomura, Katsumi Uoshima

    GERODONTOLOGY   29 ( 2 )   E703 - E709   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Objective: The purpose of this study was to subjectively evaluate the utility of newly developed denture placement and removal devices. Objective observations were also made to support the evidence.
    Materials and method: Twenty-one subjects were instructed to place and remove their dentures with and without the devices. We evaluated the device based on a questionnaire. Objective observations were based on a 2-D image analysis. We analysed three factors: the time, the area and the circumference required to insert and remove the dentures.
    Results: Image analysis showed that the effectiveness and ease with which the subject used the device significantly improved with practice. The questionnaire data showed that a majority of the subjects appreciated the device after the first and second time. While there was no significant decrease in time required to place and remove dentures even with the device, the area and circumference of the movement on 2-D images were significantly reduced.
    Conclusion: In this study, the utility of denture placement and removal devices was evaluated both subjectively and objectively. Our data reveal that the device is effective in the elderly. Further minor improvement in the device might be required to increase its effectiveness.

    DOI: 10.1111/j.1741-2358.2011.00547.x

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  • Bmp signalling in filiform tongue papillae development 査読

    Katsushige Kawasaki, Thantrira Porntaveetus, Shelly Oommen, Sarah Ghafoor, Maiko Kawasaki, Yoko Otsuka-Tanaka, James Blackburn, John A. Kessler, Paul T. Sharpe, Atsushi Ohazama

    ARCHIVES OF ORAL BIOLOGY   57 ( 6 )   805 - 813   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: Tongue papillae are critical organs in mastication. There are four different types of tongue papillae; fungiform, circumvallate, foliate, and filiform papillae. Unlike the other three taste papillae, non-gustatory papillae, filiform papillae cover the entire dorsal surface of the tongue and are important structures for the mechanical stress of sucking. Filiform papillae are further classified into two subtypes with different morphologies, depending on their location on the dorsum of the tongue. The filiform papillae at the intermolar eminence have pointed tips, whereas filiforrn papillae with rounded tips are found in other regions (anterior tongue). It remains unknown how the shape of each type of filiform papillae are determined during their development. Bmp signalling pathway has been known to regulate mechanisms that determine the shapes of many ectodermal organs. The aim of this study was to investigate the role of Bmp signalling in filiform papillae development.
    Design: Comparative in situ hybridization analysis of six Bmps (Bmp2-Bmp7) and two Bmpr genes (Bmpr1a and Bmpr1b) were carried out in filiform papillae development. We further examined tongue papillae in mice over-expressing Noggin under the keratin14 promoter (K14-Noggin).
    Results: We identified a dynamic temporo-spatial expression of Bmps in filiform papillae development. The K14-Noggin mice showed pointed filiform papillae in regions of the tongue normally occupied by the rounded type.
    Conclusions: Bmp signalling thus regulates the shape of filiform papillae. (C) 2011 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.archoralbio.2011.11.014

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  • The role of Irf6 in tooth epithelial invagination 査読

    James Blackburn, Atsushi Ohazama, Katsushige Kawasaki, Yoko Otsuka-Tanaka, Bigang Liu, Kenya Honda, Ryan B. Rountree, Yinling Hu, Maiko Kawasaki, Walter Birchmeier, Ruth Schmidt-Ullrich, Akira Kinoshita, Brian C. Schutte, Nigel L. Hammond, Michael J. Dixon, Paul T. Sharpe

    DEVELOPMENTAL BIOLOGY   365 ( 1 )   61 - 70   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Thickening and the subsequent invagination of the epithelium are an important initial step in Ectodermal organ development. Ikk alpha has been shown to play a critical role in controlling epithelial growth, since Ikk alpha mutant mice show protrusions (evaginations) of incisor tooth, whisker and hair follicle epithelium rather than invagination. We show here that mutation of the Interferon regulatory factor (11) family, Irf6 also results in evagination of incisor epithelium. In common with Ikk alpha mutants, Irf6 mutant evagination occurs in a NF-kappa B-independent manner and shows the same molecular changes as those in Ikk alpha mutants. Irf6 thus also plays a critical role in regulating epithelial invagination. In addition, we also found that canonical Wnt signaling is upregulated in evaginated incisor epithelium of both Ikk alpha and Irf6 mutant embryos. (C) 2012 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ydbio.2012.02.009

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  • Sensory and Motor Responses of Normal Young Adults During Swallowing of Foods with Different Properties and Volumes 査読

    Atsuko Igarashi, Maiko Kawasaki, Shu-ichi Nomura, Yuji Sakai, Mayumi Ueno, Ichiro Ashida, Yozo Miyaoka

    DYSPHAGIA   25 ( 3 )   198 - 206   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    We examined the influence of rheological/textural properties and volumes of test foods on the sensory and motor aspects of swallowing in healthy young adults. Three test foods differing in thickening agent concentration (0.0, 1.5, and 3.0%) were prepared and delivered in different volumes (similar to 3, similar to 5, and similar to 7 ml) to subjects seated on a chair. Viscosity analyses of the 1.5 and 3.0% test foods revealed that they behaved as non-Newtonian fluids and were thixotropic. The 1.5% test food differed from the 3.0% test food in its textural properties (hardness, cohesiveness, and adhesiveness). As determined by a linear model equation method, the thickening agent concentration affected the scores of all six sensory evaluation questions that were answered by the subjects, which suggests that the concentration affected the food properties being evaluated. Consistent with previous reports, thickening agent concentration and test food volume also affected some durational parameters of laryngeal (recorded by a piezoelectric sensor) and suprahyoid muscle (recorded on an electromyogram) motor activity. However, thickening agent concentration and test food volume did not affect the single amplitude parameter of the electromyogram that was measured. The thixotropic property of foods can affect the motor aspect of oropharyngeal swallowing as well as the sensory aspect.

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  • Effects of Hardness, Taste and Amount of Low-Gel-Strength Ager on Swallowing in Young and Elderly Subjects. 査読

    Sakai Y, Sugita K, Kawasaki M, Nomura S, Igarashi A

    J. Home Econ Jpn.   60 ( 2 )   133 - 138   2009年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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書籍等出版物

  • Innovative Research on Biosis-Abiosis Intelligent Interface

    Kawasaki M, Kawasaki K, Blackburn J, Ohazama A, Sasaki K, Suzuki O, Takahashi N( 範囲: Molecular mechanisms regulating tooth number)

    Springer Singapore  2016年 

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MISC

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講演・口頭発表等

  • The Role of Primary Cilia in Mandibular Development. 国際会議

    Kawasaki M, Kawasaki K, Ohazama A

    International Niigata-Taiwan Universitues collaborative dental research symposium  2019年 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • The Role of Primary Cilia in Craniofacial Development. 国際会議

    Maiko Kawasaki

    International Collaborative Symposium on Development of Human Resources in Practical Oral Health and treatment  2017年 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • The Role of Primary Cilia in Tongue Development. 国際会議

    Kawasaki M, Kawasaki K, Ohazama A

    Niigata-Taiwan University Dental Research Symposium  2017年 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • The Role of Primary Cilia in Tooth Development. 国際会議

    Kawasaki M, Ohazama A

    InternationalSymposium on Oral Health Education and Research  2011年 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • Comparison between implant prosthesis and removable partial denture by the mastication efficiency test and QOL survey

    Kawasaki M, Yoshida K, Kaku M, Akiba Y, Nagasawa M, Fujii N, J.M.Rosales Rocabado, Al-amin MD.Bhuiyan, Rashid MD Mamunur, Uoshima K

    Scientific Meeting of Japan Prosthodontic Society  2010年 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • Evaluation of implant prothesis by the mastication efficiency test and QOL survey.

    Kawasaki M, Uoshima K, Yoshida K, Nagasawa M, J.M. ROSALES, Al-amin B

    Scientific Meeting of Japan Prosthodontic Society  2009年 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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受賞

  • デンツプライ賞

    2010年5月   日本補綴歯科学会   咀嚼能率検査とQOLアンケートによる固定性インプラント義歯と部分床義歯の比較

    川崎真依子

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共同研究・競争的資金等の研究

  • 顎顔面の発生過程における一次繊毛の機能解明:シグナル経路のクロストークの観点から

    研究課題/領域番号:20K10092  2020年4月 - 2023年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    川崎 真依子, 前田 健康, 大峡 淳, 川崎 勝盛

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    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

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  • 意図的細胞誘導による新規エナメル上皮腫治療法開発に向けた試み

    研究課題/領域番号:18K19639  2018年6月 - 2021年3月

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    前田 健康, 大峡 淳, 川崎 勝盛, 川崎 真依子

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    配分額:6370000円 ( 直接経費:4900000円 、 間接経費:1470000円 )

    エナメル上皮腫は、歯原性腫瘍の中で最も頻度の高い疾患である。外科的処置しか選択肢がないことに加え、術後の機能障害が強くなる。罹患部位によっては著しい審美障害も伴う。また、その多くが人生を大きく左右する重要な時期である10代~20代で発症する。それは、その後の全ての生涯という長きにわたる機能的・審美的障害をも意味する。このようにエナメル上皮腫は、歯科において、克服すべき課題の極めて大きい腫瘍の一つといえる。エナメル上皮腫は歯胚上皮もしくは歯胚様細胞へと異常分化した細胞が由来となる。歯堤、星状網、エナメル上皮などの歯胚構成上皮は、最終的に非常に扁平な退縮エナメル上皮に分化後、ほとんどの活性を失い、萌出とともに体外に排出される。エナメル上皮腫細胞を退縮エナメル上皮に類似した細胞に分化誘導することによって、エナメル上皮腫の進行が抑制される可能性がある。しかしながら、これまでのエナメル芽細胞の研究は、エナメル質形成期間にのみ焦点が当てられており、退縮エナメル上皮という消失していく細胞への分化メカニズム研究は全く行われておらず、退縮エナメル上皮研究が必須となる。そこで、退縮エナメル上皮への分化誘導が制御される「成熟期エナメル芽細胞から退縮エナメル上皮までの分子変化」を解明する。Bmp one morphogenetic protein)は歯の発生に重要な役割を担うことが知られている。各種Bmpのリガンドの発現解析を行い、各リガンド特異的な発現が把握できた。

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  • 顎関節形成の包括的分子機構の解明

    研究課題/領域番号:18K09762  2018年4月 - 2021年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    川崎 勝盛, 前田 健康, 大峡 淳, 川崎 真依子

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    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

    顎関節は、顎運動の中心となる器官の一つである。その機能不全は齲蝕、歯周病に次ぐ主要な歯科領域疾病である顎関節症を引き起こす。哺乳類の顎関節は二次関節で、また歯と同一の骨にあり、動物界の中では非常に特殊なものであるため、生物学的、進化学的にも極めて意義の高い構造体である。しかし、顎関節の形成メカニズムの多くは謎のままである。一次繊毛は、長年機能の不明な細胞器官であったが、近年、この一次繊毛が様々な生命現象に関与する事が明らかとなってきた。我々は、この一次繊毛内に存在するタンパクであるIft88の神経堤由来細胞特異的欠損マウスに、過剰な顎関節形成という表現系を発見し、一次線毛を介した分子メカニズムが顎関節形成に必須であることを明らかにした。前年度、Shhシグナルの間葉組織における減少が確認されたが、そのことがIft88神経堤由来細胞特異的欠損マウスに認められた過剰な顎関節形成の原因であるか検索するために、Shhシグナルの神経堤由来細胞特異的欠損マウスを作成し観察を行ったところ、Ift88欠損マウスと同様に、顎関節の過剰な形成が確認され、Ift88欠損マウスでの顎関節の異常はShhシグナルの減少によって引き起こったことが示された。

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  • 顎関節形成の包括的分子機構の解明

    2018年 - 2020年

    日本学術振興会  基盤研究(C) 

    川崎 勝盛

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    資金種別:競争的資金

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  • 意図的細胞誘導による新規エナメル上皮腫治療法開発に向けた試み

    2018年 - 2020年

    日本学術振興会  挑戦的研究(萌芽) 

    前田健康

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    資金種別:競争的資金

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  • 口蓋突起誘導メカニズムの解明

    2017年 - 2019年

    日本学術振興会  基盤(C) 

    川崎真依子

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    担当区分:研究代表者  資金種別:競争的資金

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  • 分化機構解明による幹細胞の意図的誘導法の開発

    2017年 - 2019年

    日本学術振興会  基盤(A) 

    大峡 淳

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    資金種別:競争的資金

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  • 「生体完結型再生療法」開発への挑戦

    2017年 - 2019年

    日本学術振興会  挑戦的研究(開拓) 

    大峡 淳

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    資金種別:競争的資金

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  • 歯の再生療法に向けた幹細胞分化制御機構の解明~毛との相同性、異同性に着目して~

    2016年 - 2018年

    日本学術振興会  基盤(B) 

    前田健康

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    資金種別:競争的資金

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  • 遺伝子改変マウスを用いた歯誘導メカニズムの網羅的解析

    2016年 - 2017年

    日本学術振興会  基盤(B) 

    原田史子

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    資金種別:競争的資金

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  • エピジェネティックスから探る口唇・口蓋の発生分子機構

    2013年 - 2015年

    日本学術振興会  若手研究(B) 

    川崎真依子

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    担当区分:研究代表者  資金種別:競争的資金

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  • 抗アポトーシスタンパクHSP27の細胞内導入法を用いた効果的な骨造成法の開発

    2011年 - 2013年

    日本学術振興会  若手(B) 

    川崎真依子

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    担当区分:研究代表者  資金種別:競争的資金

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  • 抗アポトーシスタンパクHSP27の細胞内導入法を用いた効果的な骨造成法の開発

    2010年 - 2011年

    日本学術振興会  研究活動スタート支援 

    川崎真依子

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    担当区分:研究代表者  資金種別:競争的資金

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▶ 全件表示

 

担当経験のある授業科目(researchmap)

担当経験のある授業科目

  • 人体のしくみ

    2019年
    -
    現在
    機関名:新潟大学

  • 人体発生学

    2017年
    -
    現在
    機関名:新潟大学

  • 歯学研究演習

    2017年
    -
    現在
    機関名:新潟大学

  • 組織学各論

    2017年
    -
    現在
    機関名:新潟大学

  • 口腔組織発生学

    2017年
    -
    現在
    機関名:新潟大学

  • 組織学総論

    2017年
    -
    現在
    機関名:新潟大学

  • 早期臨床実習Ⅱ

    2016年
    機関名:新潟大学

  • 選択実習Ⅱ

    2010年
    機関名:新潟大学

▶ 全件表示