2021/04/21 更新

写真a

カネコ ヨシカツ
金子 佳賢
KANEKO Yoshikatsu
所属
教育研究院 医歯学系 医学系列 講師
医歯学総合研究科 腎研究センター 講師
職名
講師
外部リンク

学位

  • 博士(医学) ( 2001年3月   新潟大学 )

研究分野

  • ライフサイエンス / 内科学一般  / 老年医学

  • ライフサイエンス / 腎臓内科学

  • ライフサイエンス / 免疫学

経歴

  • 新潟大学   医歯学総合研究科 腎研究センター   講師

    2016年4月 - 現在

  • 新潟大学   医歯学総合研究科 生体機能調節医学専攻 内部環境医学   講師

    2015年4月 - 2016年3月

  • 新潟大学   医歯学総合研究科 生体機能調節医学専攻 内部環境医学   助教

    2014年8月 - 2015年3月

  • 新潟大学   医歯学総合病院 腎・膠原病内科   助教

    2012年11月 - 2014年7月

  • 新潟大学   第二内科   助教

    2011年5月 - 2012年11月

  • 新潟大学   医歯学総合研究科   特任助教

    2010年10月 - 2011年4月

▶ 全件表示

取得資格

  • 医師

 

論文

  • Mood Disorder in Systemic Lupus Erythematosus Induced by Antiribosomal P Protein Antibodies Associated with Decreased Serum and Brain Tryptophan 査読

    Takamasa Cho, Hiroe Sato, Ayako Wakamatsu, Riuko Ohashi, Yoichi Ajioka, Toshio Uchiumi, Shin Goto, Ichiei Narita, Yoshikatsu Kaneko

    The Journal of Immunology   206 ( 8 )   1729 - 1739   2021年4月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:The American Association of Immunologists  

    DOI: 10.4049/jimmunol.2000260

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  • Brain‐derived neurotrophic factor is associated with sarcopenia and frailty in Japanese hemodialysis patients 査読

    Satoru Miyazaki, Noriaki Iino, Ryo Koda, Ichiei Narita, Yoshikatsu Kaneko

    Geriatrics & Gerontology International   21 ( 1 )   27 - 33   2021年1月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/ggi.14089

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/ggi.14089

  • Attenuated Macrophage Infiltration in Glomeruli of Aged Mice Resulting in Ameliorated Kidney Injury in Nephrotoxic Serum Nephritis. 査読

    Kaneko Y, Cho T, Sato Y, Goto K, Yamamoto S, Goto S, Madaio MP, Narita I

    The journals of gerontology. Series A, Biological sciences and medical sciences   73 ( 9 )   1178 - 1186   2018年8月

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    担当区分:筆頭著者, 責任著者  

    DOI: 10.1093/gerona/gly019

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  • Kidney morphological parameters measured using noncontrast-enhanced steady-state free precession MRI with spatially selective inversion recovery pulse correlate with eGFR in patients with advanced CKD 査読

    Tadashi Otsuka, Yoshikatsu Kaneko, Yuya Sato, Ryohei Kaseda, Ryuji Aoyagi, Suguru Yamamoto, Shin Goto, Ichiei Narita

    Clinical and Experimental Nephrology   22 ( 1 )   45 - 54   2018年2月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Tokyo  

    Background: It is well known that atrophic renal changes are associated with chronic kidney disease (CKD) progression, but conventional diagnostic imaging methods such as noncontrast-enhanced computed tomography and magnetic resonance imaging (MRI) have been insufficient for precisely assessing kidney function because they cannot clearly distinguish between the medulla and cortex. Hence, here we used noncontrast-enhanced steady-state free precession (SSFP) MRI with a spatially selective inversion recovery (IR) pulse to improve visibility for renal corticomedullary differentiation and evaluated the association between morphological parameters and kidney function in patients with CKD. Methods: Kidney corticomedullary contrast ratio, cortical and medullary areas, and minimal cortical thickness of 107 patients with CKD G1–G5 were measured using SSFP MRI with a spatially selective IR pulse and the association between these morphological parameters and kidney function were evaluated. Results: Corticomedullary contrast ratio was significantly improved on SSFP MRI compared with conventional in-phase T1-weighted gradient-echo MRI and positively correlated with estimated glomerular filtration ratio (eGFR), raw eGFR, and 24-h creatinine clearance. The medullary and cortical areas and minimal cortical thickness also positively correlated with those of kidney functional markers and the age. In patients with CKD and diabetes mellitus (DM), the correlation coefficients between raw eGFR and morphological parameters were higher than those in patients without DM, while minimal cortical thickness was larger in CKD patients with DM with a raw eGFR ≥ 45 mL/min. Conclusion: Kidney morphological parameters measured with SSFP MRI were clearly correlated with kidney function in patients with CKD, including those with advanced kidney dysfunction.

    DOI: 10.1007/s10157-017-1413-x

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  • Prolactin Upregulates Female-Predominant P450 Gene Expressions and Downregulates Male-Predominant Gene Expressions in Mouse Liver 査読

    Yuya Sato, Yoshikatsu Kaneko, Takamasa Cho, Kei Goto, Tadashi Otsuka, Suguru Yamamoto, Shin Goto, Hiroki Maruyama, Ichiei Narita

    DRUG METABOLISM AND DISPOSITION   45 ( 6 )   586 - 592   2017年6月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS  

    Prolactin is a polypeptide hormone with over 300 separate biologic activities. Its serum level is increased during pregnancy and lactation, and it has been reported that pregnancy and lactation affect drug and steroid metabolism in mice and humans. Several studies reported that pregnancy or lactation influences liver cytochrome P450 (P450) expression and its activity, affecting the biosynthesis of steroids and xenobiotics through growth hormone or sex hormones; however, the role of prolactin as the regulator of liver P450 expression has not been elucidated so far. In the present study, we focused on prolactin as the regulator of expression of liver sex-predominant genes, including P450s. To investigate the role of prolactin in the hepatic gene expressions, pCAGGS expression vector containing mouse prolactin cDNA was transfected by hydrodynamic injection into both male and female mice. Hyperprolactinemia phosphorylated signal transducer and activator of transcription 5 in the liver and augmented female mouse liver mRNA expression of Cyp3a16, Cyp3a41, Cyp3a44, Cyp2b9, and prolactin receptor genes, whose expressions were female-predominant in hepatocytes. Moreover, liver expression of male-predominant genes such as Cyp2d9, Cyp7b1, Mup1, and Alas2 were reduced in male mice with hyper-prolactinemia. The serum levels of conventional regulators of hepatic gene expressions, growth hormone, and testosterone were not affected by hyperprolactinemia. We demonstrated that prolactin upregulated female-predominant genes in female mice and downregulated male-predominant genes in male mice. We conjecture that higher concentration of prolactin would alter steroid and xenobiotic metabolisms by modulating hepatic P450 gene expressions during pregnancy and lactation.

    DOI: 10.1124/dmd.116.074658

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  • Extracapillary proliferation and arteriolar hyalinosis are associated with long-term kidney survival in IgA nephropathy 査読

    Yoshikatsu Kaneko, Kazuhiro Yoshita, Emiko Kono, Yumi Ito, Naofumi Imai, Suguru Yamamoto, Shin Goto, Ichiei Narita

    CLINICAL AND EXPERIMENTAL NEPHROLOGY   20 ( 4 )   569 - 577   2016年8月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    The Oxford classification of IgA nephropathy consists of four markers as prognosticators. We retrospectively examined the relevance of extracapillary proliferation involving cellular and fibrocellular crescents (Ex) and arteriolar hyalinosis (A) on the long-term outcome of renal function.
    A total of 314 Japanese patients who were diagnosed with IgA nephropathy, with 12 months or more of follow-up period were included in this study. A total of 186 patients were with UP aeyen 0.5 g/day. Patients with diabetes mellitus or severe kidney injury (eGFR < 30 ml/min/1.73 m(2)) were excluded. The presence of Ex and A were scored 0 in the absence, and 1 in the presence, of each lesion. The end point was determined as a 50 % reduction in initial eGFR or end-stage renal disease defined as eGFR < 15 ml/min/1.73 m(2).
    In univariate analyses, the kidney survival rate was significantly lower in patients with Ex1 and A1 if UP aeyen 0.5 g/day. In the patients with UP < 0.5/day, none of the clinical and pathological parameters was determined as a risk factor. In the multivariate model including pathological parameters, Ex1 and A1 were independent risk factors for renal outcome if UP aeyen 0.5 g/day. In those patients treated with RAS-blocker or treated before introduction of methylprednisolone pulse therapy, Ex was the only independent risk factor. In multivariate analysis including clinical parameters, eGFR alone was a risk factor, due to strong correlation with other parameters.
    Ex and A would be associated with the renal outcome of the patients with UP aeyen 0.5 g/day.

    DOI: 10.1007/s10157-015-1185-0

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  • Leptin deficiency down-regulates IL-23 production in glomerular podocytes resulting in an attenuated immune response in nephrotoxic serum nephritis 査読

    Kei Goto, Yoshikatsu Kaneko, Yuya Sato, Tadashi Otsuka, Suguru Yamamoto, Shin Goto, Keiko Yamamoto, Tadashi Yamamoto, Hiroshi Kawachi, Michael P. Madaio, Ichiei Narita

    INTERNATIONAL IMMUNOLOGY   28 ( 4 )   197 - 208   2016年4月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Leptin and IL-23 production in NTS nephritis.Leptin, one of the typical adipokines, is reported to promote T(h)17 cell responses and to enhance production of proinflammatory cytokines. To clarify the role of leptin in the regulation of the IL-23/IL-17 axis and the development of kidney disease, we used a murine model of nephrotoxic serum (NTS) nephritis (NTN). Sheep NTS was administered in wild-type C57BL/6J mice and food-restricted, leptin-deficient C57BL/6J-ob/ob (FR-ob/ob) mice after preimmunization with sheep IgG. The profile of mRNA expression relevant to T helper lymphocytes in the kidneys was analyzed by quantitative real-time PCR (qRT-PCR). Cultured murine glomerular podocytes and peritoneal exudate macrophages (PEMs) were used to investigate the direct effect of leptin on IL-23 or MCP-1 production by qRT-PCR. Kidney injury and macrophage infiltration were significantly attenuated in FR-ob/ob mice 7 days after NTS injection. The T(h)17-dependent secondary immune response against deposited NTS in the glomeruli was totally impaired in FR-ob/ob mice because of deteriorated IL-17 and proinflammatory cytokine production including IL-23 and MCP-1 in the kidney. IL-23 was produced in glomerular podocytes in NTN mice and cultured murine glomerular podocytes produced IL-23 under leptin stimulation. MCP-1 production in PEMs was also promoted by leptin. Induction of MCP-1 expression was observed in PEMs regardless of Ob-Rb, and the leptin signal was transduced without STAT3 phosphorylation in PEMs. Leptin deficiency impairs the secondary immune response against NTS and down-regulates IL-23 production and T(h)17 responses in the NTN kidney, which is accompanied by decreased MCP-1 production and macrophage infiltration in the NTN kidney.

    DOI: 10.1093/intimm/dxv067

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  • Upregulation of prolactin receptor in proximal tubular cells was induced in cardiac dysfunction model mice 査読

    Yohei Tsuchida, Yoshikatsu Kaneko, Tadashi Otsuka, Kei Goto, Akihiko Saito, Keiko Yamamoto, Tadashi Yamamoto, Ichiei Narita

    CLINICAL AND EXPERIMENTAL NEPHROLOGY   18 ( 1 )   65 - 74   2014年2月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    In order to clarify the interaction between cardiac dysfunction and sodium homeostasis in the kidney, we used a murine model of cardiac dysfunction and investigated the effect on sodium transporters in renal tubular cells.
    Cardiac function was deteriorated by abdominal aortic banding, and the gene expression of sodium transporters in the kidneys was evaluated by real-time RT-PCR and compared with that in the kidneys of control mice.
    Gene expression of all three variants of the murine prolactin receptor was enhanced by aortic banding. Upregulated prolactin receptor was distributed in the proximal tubular cells of the pars recta in the deep inner cortex and the outer stripe of the outer medulla. Prolactin has been reported to be a natriuretic hormone that inhibits proximal tubular Na+/K+-ATPase activity, resulting in reduced sodium reabsorption and the acceleration of natriuresis. Inhibition of endogenous prolactin secretion by bromocriptine administration decreased the urine sodium excretion in both aortic banding and control mice. On the other hand, excess exogenous prolactin administration enhanced urine potassium excretion in aortic banding mice. Furthermore, a high-sodium diet accelerated urinary sodium excretion, which was also significantly decreased by inhibition of endogenous prolactin secretion in aortic banding mice.
    We reported that the prolactin receptor was upregulated by aortic banding treatment. Prolactin-prolactin receptor interaction in the proximal tubular cells of the pars recta should involve a different mechanism of kaliuresis other than inhibition of Na+/K+-ATPase.

    DOI: 10.1007/s10157-013-0820-x

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  • Integrin α1/β1 and α2/β1 as a receptor for IgA1 in human glomerular mesangial cells in IgA nephropathy. 査読

    Kaneko Y, Otsuka T, Tsuchida Y, Gejyo F, Narita I

    International immunology   24 ( 4 )   219 - 232   2012年4月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/intimm/dxr125

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  • Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation 査読

    Yoshikatsu Kaneko, Falk Nimmerjahn, Jeffrey V. Ravetch

    SCIENCE   313 ( 5787 )   670 - 673   2006年8月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC ADVANCEMENT SCIENCE  

    Immunoglobulin G (IgG) mediates pro- and anti-inflammatory activities through the engagement of its Fc fragment (Fc) with distinct Fc gamma receptors (Fc gamma Rs). One class of Fc-Fc gamma R interactions generates pro-inflammatory effects of immune complexes and cytotoxic antibodies. In contrast, therapeutic intravenous gamma globulin and its Fc fragments are anti-inflammatory. We show here that these distinct properties of the IgG Fc result from differential sialylation of the Fc core polysaccharide. IgG acquires anti-inflammatory properties upon Fc sialylation, which is reduced upon the induction of an antigen-specific immune response. This differential sialylation may provide a switch from innate anti-inflammatory activity in the steady state to generating adaptive pro-inflammatory effects upon antigenic challenge.

    DOI: 10.1126/science.1129594

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  • Pathology and protection in nephrotoxic nephritis is determined by selective engagement of specific Fc receptors 査読

    Y Kaneko, F Nimmerjahn, MP Madaio, JV Ravetch

    JOURNAL OF EXPERIMENTAL MEDICINE   203 ( 3 )   789 - 797   2006年3月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ROCKEFELLER UNIV PRESS  

    Introduction of heterologous anti-glomerular basement membrane antiserum (nephrotoxic serum, NTS) into presensitized mice triggers the production of IgG anti-NTS antibodies that are predominantly IgG2b and the glomerular deposition of pathogenic immune complexes, leading to accelerated renal disease. The pathology observed in this model is determined by the effector cell activation threshold that is established by the coexpression on infiltrating macrophages of the IgG2a/2b restricted activation receptor Fc gamma RIV and its inhibitory receptor counterpart, Fc gamma RIIB. Blocking Fc gamma RIV with a specific monoclonal antibody thereby preventing IgG2b engagement or treatment with high dose intravenous gamma-globulin (IVIG) to down-regulate Fc gamma RIV while up-regulating Fc gamma RIIB, protects mice from fatal disease. In the absence of Fc gamma RIIB, IVIG is not protective; this indicates that reduced Fc gamma RIV expression alone is insufficient to protect animals from pathogenic IgG2b immune complexes. These results establish the significance of specific IgG subclasses and their cognate FcR gamma s in renal disease.

    DOI: 10.1084/jem.20051900

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  • Monocyte chemoattractant protein-1 A-2518G gene polymorphism and renal survival of Japanese patients with immunoglobulin A nephropathy 査読

    Honami Mori, Yoshikatsu Kaneko, Ichiei Narita, Shin Goto, Noriko Saito, Daisuke Kondo, Fuminori Sato, Junya Ajiro, Daisuke Saga, Asa Ogawa, Minoru Sakatsume, Mitsuhiro Ueno, Kaoru Tabei, Fumitake Gejyo

    Clinical and Experimental Nephrology   9 ( 4 )   297 - 303   2005年12月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background. Monocyte chemoattractant protein (MCP)-1 is closely related to the pathogenesis of the progression of various chronic renal diseases, including IgA nephropathy (IgAN), through its chemoattractant effect on macrophages. However, the correlation of MCP-1 gene polymorphism with the long-term prognosis of Japanese patients with IgAN has not been clearly determined yet. Methods. We investigated 277 Japanese patients diagnosed with IgAN based on renal biopsy to clarify the association between the progression of IgAN and MCP-1 gene polymorphism at position A-2518G, which regulates the transcription of the MCP-1 gene. Results. The incidence of endstage renal disease was significantly higher in patients with the AA genotype (47.1%) compared to those with the AG (24.1%) or GG (27.4%) genotype (P = 0.024). Moreover, Kaplan-Meier analysis revealed that the AA genotype significantly facilitated the progression of renal disease (log rank
    P = 0.0029), and Cox proportional hazards regression model analysis showed that the AA genotype represented a 2.058-fold risk for the progression of renal disease (P = 0.026) compared to the AG/GG genotype. However, when the patients were treated with angiotensin-converting enzyme inhibitor and/or angiotensin receptor blocker, or corticosteroid, homozygosity for the -2518A allele was not associated with a higher rate of incidence of endstage renal disease. Serum MCP-1 levels were higher although not significantly so, in the patients with IgAN possessing the AA genotype. Conclusions. The AA genotype at MCP-1 -2518 was an independent risk factor for the progression of renal disease in Japanese patients with IgAN, and was closely associated with renal survival. © Japanese Society of Nephrology 2005.

    DOI: 10.1007/s10157-005-0375-6

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  • Natural saline-flush is sufficient to maintain patency of immobilized-urokinase double-lumen catheter used to provide temporary blood access for hemodialysis 査読

    Y Kaneko, M Iwano, H Yoshida, M Kosuge, S Ito, Narita, I, F Gejyo, M Suzuki

    BLOOD PURIFICATION   22 ( 5 )   473 - 479   2004年

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    Background: Thrombotic occlusion is a frequent complication of central venous catheters used to provide temporary blood access on hemodialysis therapy. Heparin-lock is conventionally used to maintain patency of the catheter, but the necessity of heparin-lock has not been determined yet. Methods: After the immobilized-urokinase double-lumen central venous catheter was inserted into 48 Japanese hemodialysis patients, 22 patients randomized to the heparin group received a 20-ml saline-flush, followed by 2 ml of 1,000 U/ml heparin-lock, and 26 patients randomized to the saline group received only the 20-ml saline-flush once a day for each lumen. Results: Thrombotic occlusion was observed in only 1 out of 22 patients in the heparin group and 1 out of 26 patients in the saline group. No significant difference of the catheter survival was observed between the two groups (p = 0.8599). Conclusions: Natural saline-flush is sufficient for maintaining the patency of an immobilized-urokinase double-lumen central venous catheter. Copyright (C) 2004 S. Karger AG, Basel.

    DOI: 10.1159/000081811

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  • Transferrin saturation versus reticulocyte hemoglobin content for iron deficiency in Japanese hemodialysis patients 査読

    Y Kaneko, S Miyazaki, Y Hirasawa, F Gejyo, M Suzuki

    KIDNEY INTERNATIONAL   63 ( 3 )   1086 - 1093   2003年3月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING INC  

    Background. Iron deficiency is a frequent cause of recombinant human erythropoietin (rhEPO)-resistant anemia in hemodialysis patients. Both reticulocyte hemoglobin content (CHr) and transferrin saturation (TSAT) have been proposed as markers of iron deficiency, but it is unclear which parameter is superior.
    Methods. To compare the efficacy of CHr and TSAT as an indicator for treatment of iron deficiency, we conducted a single-center, open-label, prospective, randomized, controlled trial at the Kidney Center in Shinraku-en Hospital of 197 Japanese patients on chronic hemodialysis. After 4 weeks of run-in period during which iron supplementation was suspended, 100 patients who were randomized to the CHr group received 240 mg iron colloid intravenously over 2 weeks when CHr less than 32.5 pg, and 97 patients who were randomized to the TSAT group received the same doses of iron colloid when TSAT less than 20%. We measured the rhEPO dose needed to maintain prestudy hematocrit levels, hematocrit, CHr, TSAT, serum ferritin, percentage of hypochromic red blood cells, and total iron administered.
    Results. Sixteen weeks later, 94 patients in the CHr group and 89 patients in the TSAT group finished the study. The doses of rhEPO required decreased by 35.8% (4081 to 2629 U/week, P < group (4121 to 3606 U/week). Although CHr increased promptly after the iron administration in both groups, TSAT increased only in the TSAT group.
    Conclusions. Although CHr reflects the iron status more sensitively, TSAT is a better clinical marker for iron supplementation therapy.

    DOI: 10.1046/j.1523-1755.2003.00826.x

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  • Macrophage metalloelastase as a major factor for glomerular injury in anti-glomerular basement membrane nephritis 査読

    Y Kaneko, M Sakatsume, YS Xie, T Kuroda, M Igashima, Narita, I, F Gejyo

    JOURNAL OF IMMUNOLOGY   170 ( 6 )   3377 - 3385   2003年3月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC IMMUNOLOGISTS  

    Rat anti-glomerular basement membrane (GBM) nephritis is a model of crescentic glomerulonephritis induced by injection of anti-GBM antiserum. To elucidate the mechanism of glomerular injury, we analyzed the gene expression patterns in the kidneys of anti-GBM nephritis rats using DNA arrays, and found that macrophage metalloelastase/matrix metalloproteinase (MMP)-12 was one of the highly expressed genes in the kidneys on days 3 and 7 after the injection of anti-GBM antiserum. Enhancement of MMP-12 mRNA expression was confirmed by Northern blot analysis, and in situ hybridization revealed that MMP-12 mRNA was expressed in ED-1-positive macrophages and multinuclear giant cells in the glomeruli with crescent. Moreover, these cells were positive with anti-rat rMMP-12 Ab on the section of the kidneys of anti-GBM nephritis rats on day 7. To clarify the role of MMP-12, we conducted a neutralization experiment using anti-rat rMMP-12 Ab, which had an ability to inhibit rMMP-12 activity of degrading natural substrate such as bovine, elastin or human fibronectin in vitro. Anti-rat rMMP-12 Ab or control Ig was injected in each of six rats on days 0, 2, 4, and 6 after the injection of anti-GBM antiserum. Consequently, crescent formation and macrophage infiltration in the glomeruli were significantly reduced in the rats treated with anti-rat rMMP-12 Ab, and the amount of urine protein was also decreased. These results disclosed that MMP-12 played an important role in glomerular injury in a crescentic glomerulonephritis model, and inhibition of MMP-12 may lead to a new therapeutic strategy for this disease.

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  • Augmentation of Vα14 NKT cell-mediated cytotoxicity by interleukin 4 in an autocrine mechanism resulting in the development of concanavalin A-induced hepatitis. 査読

    Yoshikatsu Kaneko, Michishige Harada, Tetsu Kawano, Masakatsu Yamashita, Youichi Shibata, Fumitake Gejyo, Toshinori Nakayama, Masaru Taniguchi

    Journal of Experimental Medicine   191 ( 1 )   105 - 114   2000年1月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1084/jem.191.1.105

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  • Antitumor cytotoxicity mediated by ligand-activated human Vα24 NKT cells. 査読

    Tetsu Kawano, Toshinori Nakayama, Noriaki Kamata, Yoshikatsu Kaneko, Michishige Harada, Nobutaka Ogura, Yasunori Akutsu, Shinichiro Motohashi, Toshihiko Iizasa, Hideharu Endo, Takehiko Fujisawa, Hiroshi Shinkai, Masaru Taniguchi

    Cancer Research   59 ( 20 )   5102 - 5105   1999年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • A novel recognition motif of human NKT antigen receptor for a glycolipid ligand 査読

    T Kawano, Y Tanaka, E Shimizu, Y Kaneko, N Kamata, H Sato, H Osada, S Sekiya, T Nakayama, M Taniguchi

    INTERNATIONAL IMMUNOLOGY   11 ( 6 )   881 - 887   1999年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Murine NKT cells can recognize a-galactosylceramide (alpha-GalCer) in the context of a class Ib CD1d molecule. Here we show that alpha-GalCer can selectively activate freshly isolated human V(alpha)24(+)V(beta)11 (+) cells, functionally defining the human NKT cells. The naive human NKT cell repertoire consisted of cells expressing an invariant V(alpha)24J(alpha)Q chain and a diverse array of beta chains derived from a single V beta 11 gene segment. Stimulation with a-GalCer expanded a polyclonal subset of the human NKT cell repertoire carrying a novel complementarity-determining region (CDR) 3 beta consensus motif that may directly interact with the sugar moiety of a-GalCer, Our data suggest that certain redundancy is allowed for CDR3 beta of NKT antigen receptor to interact with the ligand and provide a first clue to understand the novel protein-carbohydrate interaction mechanisms.

    DOI: 10.1093/intimm/11.6.881

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  • Natural killer-like nonspecific tumor cell lysis mediated by specific ligand-activated Vα14 NKT cells. 査読

    Tetsu Kawano, Junquing Cui, Yasuhiko Koezuka, Isao Toura, Yoshikatsu Kaneko, Hiroshi Sato, Eisuke Kondo, Michishige Harada, Haruhiko Koseki, Toshinori Nakayama, Yujiro Tanaka, Masaru Taniguchi

    Proceedings of the National Academy of Sciences of the United States of America   95 ( 10 )   5690 - 5693   1998年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1073/pnas.95.10.5690

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  • Requirement for Vα14 NKT cells in IL-12-mediated rejection of tumors. 査読

    Junquing Cui, Tahiro Shin, Tetsu Kawano, Hiroshi Sato, Eisuke Kondo, Isao Toura, Yoshikatsu Kaneko, Haruhiko Koseki, Masamoto Kanno, Masaru Taniguchi

    Science   278 ( 5343 )   1623 - 1626   1997年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1126/science.278.5343.1623

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  • CD1d-restricted and TCR-mediated activation of Vα14 NKT cells by glycosylceramides. 査読

    Tetsu Kawano, Junquing Cui, Yasuhiko Koezuka, Isao Toura, Yoshikatsu Kaneko, Kazuhiro Motoki, Hitomi Ueno, Ryusuke Nakagawa, Hiroshi Sato, Eisuke Kondo, Haruhiko Koseki, Masaru Taniguchi

    Science   278 ( 5343 )   1626 - 1629   1997年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1126/science.278.5343.1626

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  • Association of coexisting anti-ribosomal P and anti-dsDNA antibodies with histology and renal prognosis in lupus nephritis patients 査読

    Ayako Wakamatsu, Hiroe Sato, Yoshikatsu Kaneko, Takamasa Cho, Yumi Ito, Yoichi Kurosawa, Eriko Hasegawa, Daisuke Kobayashi, Takeshi Nakatsue, Takeshi Kuroda, Yoshiki Suzuki, Toshio Uchiumi, Ichiei Narita

    Lupus   096120332098390 - 096120332098390   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    <sec><title>Objectives</title> Anti-ribosomal P protein autoantibodies (anti-P) specifically develop in patients with systemic lupus erythematosus. Associations of anti-P with lupus nephritis (LN) histological subclass and renal outcome remain inconclusive. We sought to determine the association of anti-P and anti-double-stranded DNA antibody (anti-dsDNA) with renal histology and prognosis in LN patients.

    </sec><sec><title>Methods</title> Thirty-four patients with LN, having undergone kidney biopsy, were included. The 2018 revised ISN/RPS classification system was used for pathophysiological evaluation. Chronic kidney disease (CKD) was defined as an estimated glomerular filtration rate &lt; 60 mL/min/1.73 m<sup>2</sup> for &gt; 3 months.

    </sec><sec><title>Results</title> Six patients (17.6%) were positive for anti-P and 26 (76.5%) for anti-dsDNA. Among the six patients with anti-P, one did not have anti-dsDNA, but did have anti-Sm antibody, and showed a histological subtype of class V. This patient maintained good renal function for over 14 years. The remaining five patients, who had both anti-P and anti-dsDNA, exhibited proliferative nephritis and were associated with prolonged hypocomplementemia, and the incidence of CKD did not differ from patients without anti-P.

    </sec><sec><title>Conclusion</title> Although this study included a small number of patients, the results indicated that histology class and renal prognosis associated with anti-P depend on the coexistence of anti-dsDNA. Further studies with a large number of patients are required to confirm this conclusion.

    </sec>

    DOI: 10.1177/0961203320983906

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    その他リンク: http://journals.sagepub.com/doi/full-xml/10.1177/0961203320983906

  • Aberrant mucosal immunoreaction to tonsillar microbiota in immunoglobulin A nephropathy 査読

    Hiroki Yamaguchi, Shin Goto, Nao Takahashi, Masafumi Tsuchida, Hirofumi Watanabe, Suguru Yamamoto, Yoshikatsu Kaneko, Koichi Higashi, Hiroshi Mori, Yukio Nakamura, Arata Horii, Ken Kurokawa, Ichiei Narita

    Nephrology Dialysis Transplantation   36 ( 1 )   75 - 86   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    <title>Abstract</title>
    <sec>
    <title>Background</title>
    Immunoglobulin A nephropathy (IgAN) is the most common glomerulonephritis worldwide, characterized by mesangial polymeric IgA1 deposition. IgAN is believed to develop owing to aberrant mucosal immunoreaction against commensals in the tonsils. However, the exact interrelation between pathogenic IgA and mucosal microbiota in IgAN patients is unclear.


    </sec>
    <sec>
    <title>Methods</title>
    Biopsy-proven IgAN or recurrent tonsillitis (RT) patients who had undergone tonsillectomy were enrolled. We used 16S ribosomal RNA gene amplicon sequencing with a flow cytometry-based bacterial cell sorting technique) and immunoglobulin repertoire sequencing of the IgA heavy chain to characterize IgA-coated bacteria of the tonsillar microbiota (IgA-SEQ) and their corresponding IgA repertoire. Furthermore, we fractionated patient serum using gel-filtration chromatography and performed flow cytometry-based analysis of IgA binding to bacteria cultured from incised tonsils.


    </sec>
    <sec>
    <title>Results</title>
    Tonsillar proliferation-inducing ligand and B-cell activating factor levels were significantly higher in IgAN than in RT patients. IgA-SEQ for tonsillar microbiota revealed the preferential binding ability of IgA to Bacteroidetes in IgAN tonsils compared with those from RT patients. Expression of immunoglobulin heavy (IGH) constant alpha 1 with IGH variable 3–30 was significantly higher in IgAN than that in RT, and positively correlated with the IgA-coated enrichment score of Bacteroidetes. Serum polymeric IgA, comprising high levels of GdIgA1, exhibited considerable binding to Bacteroidetes strains cultured from the tonsils of IgAN patients.


    </sec>
    <sec>
    <title>Conclusions</title>
    These findings provide evidence that aberrant mucosal immune responses to tonsillar anaerobic microbiota, primarily consisting of members of the phylum Bacteroidetes, are involved in IgAN pathophysiology.


    </sec>

    DOI: 10.1093/ndt/gfaa223

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  • Inorganic polyphosphate potentiates lipopolysaccharide-induced macrophage inflammatory response. 査読 国際誌

    Toru Ito, Suguru Yamamoto, Keiichi Yamaguchi, Mami Sato, Yoshikatsu Kaneko, Shin Goto, Yuji Goto, Ichiei Narita

    The Journal of biological chemistry   295 ( 12 )   4014 - 4023   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Inorganic polyphosphate (polyP) is a linear polymer of orthophosphate units that are linked by phosphoanhydride bonds and is involved in various pathophysiological processes. However, the role of polyP in immune cell dysfunction is not well-understood. In this study, using several biochemical and cell biology approaches, including cytokine assays, immunofluorescence microscopy, receptor-binding assays with quartz crystal microbalance, and dynamic light scanning, we investigated the effect of polyP on in vitro lipopolysaccharide (LPS)-induced macrophage inflammatory response. PolyP up-regulated LPS-induced production of the inflammatory cytokines, such as tumor necrosis factor α, interleukin-1β, and interleukin-6, in macrophages, and the effect was polyP dose- and chain length-dependent. However, orthophosphate did not exhibit this effect. PolyP enhanced the LPS-induced intracellular macrophage inflammatory signals. Affinity analysis revealed that polyP interacts with LPS, inducing formation of small micelles, and the polyP-LPS complex enhanced the binding affinity of LPS to Toll-like receptor 4 (TLR4) on macrophages. These results suggest that inorganic polyP plays a critical role in promoting inflammatory response by enhancing the interaction between LPS and TLR4 in macrophages.

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  • Indoxyl Sulfate Promotes Macrophage IL-1β Production by Activating Aryl Hydrocarbon Receptor/NF-κ/MAPK Cascades, but the NLRP3 inflammasome Was Not Activated. 査読

    Wakamatsu T, Yamamoto S, Ito T, Sato Y, Matsuo K, Takahashi Y, Kaneko Y, Goto S, Kazama JJ, Gejyo F, Narita I

    Toxins   10 ( 3 )   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/toxins10030124

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  • Adsorption of Protein-Bound Uremic Toxins Through Direct Hemoperfusion With Hexadecyl-Immobilized Cellulose Beads in Patients Undergoing Hemodialysis 査読

    Suguru Yamamoto, Mami Sato, Yoko Sato, Takuya Wakamatsu, Yoshimitsu Takahashi, Akira Iguchi, Kentaro Omori, Yasushi Suzuki, Isei Ei, Yoshikatsu Kaneko, Shin Goto, Junichiro J. Kazama, Fumitake Gejyo, Ichiei Narita

    Artificial Organs   42 ( 1 )   88 - 93   2018年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/aor.12961

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  • Comparison of methods of steroid administration combined with tonsillectomy for IgA nephropathy patients 査読

    Hirofumi Watanabe, Shin Goto, Daisuke Kondo, Takuma Takata, Hajime Yamazaki, Michihiro Hosojima, Suguru Yamamoto, Yoshikatsu Kaneko, Ryuji Aoyagi, Ichiei Narita

    CLINICAL AND EXPERIMENTAL NEPHROLOGY   21 ( 2 )   257 - 265   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    IgA nephropathy (IgAN) is a chronic glomerular disease that causes end-stage renal disease in 20-40 % of patients within 20 years. The efficacy of tonsillectomy combined with steroid pulse (SP) administration (TSP) for clinical remission of IgAN has been reported. Particularly in Japan, TSP has been performed widely. However, the optimum method for steroid administration in TSP has not been established.
    We retrospectively compared clinical remission in IgAN patients treated with tonsillectomy combined with two different steroid administration methods: (1) three courses of SP therapy and oral prednisolone administered on alternate days (group 3A; n = 25); and (2) one course of SP therapy and oral prednisolone administered on consecutive days (group 1C; n = 22).
    There was no significant difference in the clinical remission rates between the two groups at 12 (48.0 vs. 40.9 %, P = 0.77) and 24 months after starting treatment (68.0 vs. 72.7 %, P = 0.76) and at the final observation (76.0 vs. 81.8 %, P = 0.73). The mean period from starting treatment to remission of hematuria in group 3A was significantly shorter than that in group 1C (5.7 +/- 4.4 vs. 9.9 +/- 5.9 months, P = 0.03). Dyslipidemic patients treated for the first time with statin after the SP therapy were more present in group 3A at 24 months (P = 0.02).
    In IgAN patients, treatment of group 3A may be effective for inducing rapid remission of hematuria. Further studies are needed to establish an appropriate protocol for TSP.

    DOI: 10.1007/s10157-016-1282-8

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  • Hemodiafiltration for hepatic encephalopathy induced by Budd-Chiari syndrome in a patient with end-stage kidney disease. 査読

    Wakamatsu T, Yamamoto S, Kamimura K, Nakatsue T, Iino N, Iguchi S, Kaneko Y, Goto S, Kazama JJ, Narita I

    CEN case reports   5 ( 2 )   125 - 130   2016年11月

  • Is IgA nephropathy (IgAN) a familial or sporadic disease? 査読

    Ichiei Narita, Yoshikatsu Kaneko, Yumi Itoh, Yuichi Sakamaki, Seitaro Iguchi, Suguru Yamamoto, Minako Wakasugi, Junichiro J. Kazama, Shin Goto

    Pathogenesis and Treatment in IgA Nephropathy: An International Comparison   43 - 51   2016年3月

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer Japan  

    There have been several lines of evidences for familial aggregation of IgA nephropathy (IgAN), as well as mesangial deposition of IgA, suggesting that the susceptibility to this disease is genetically controlled. In our institute, family histories of hematuria, end-stage kidney disease, and glomerulonephritis are observed in about 10 % of cases with IgAN, even in those without any significant hereditary nephritis or kidney diseases. Recent large-scale genome-wide association studies (GWAS) of sporadic IgAN have identified multiple susceptibility loci, providing an insight into the genetic architecture of this disease, although each of their individual impact to the development of the disease is still not enough. It has been recognized that most of these loci are either directly associated with risk of inflammatory bowel disease (IBD) or maintenance of the intestinal epithelial barrier and response to mucosal pathogens. Further elucidation of the role of genetic variants underlying IgAN, and hologenetic views of gene variants and environmental factors, would be necessary to understand the precise pathogenic mechanism of IgAN in more detail.

    DOI: 10.1007/978-4-431-55588-9_3

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  • [112th Scientific Meeting of the Japanese Society of Internal Medicine: Educational Lecture: The Pathophysiology of IgA Nephropathy: Recent Advancement and Perspectives]. 査読

    Narita I, Goto S, Kaneko Y

    Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine   104 ( 9 )   1930 - 1936   2015年9月

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  • Increased Proinflammatory Cytokine Production and Decreased Cholesterol Efflux Due to Downregulation of ABCG1 in Macrophages Exposed to Indoxyl Sulfate 査読

    Koji Matsuo, Suguru Yamamoto, Takuya Wakamatsu, Yoshimitsu Takahashi, Kazuko Kawamura, Yoshikatsu Kaneko, Shin Goto, Junichiro J. Kazama, Ichiei Narita

    TOXINS   7 ( 8 )   3155 - 3166   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    One of the possible causes of enhanced atherosclerosis in patients with chronic kidney disease (CKD) is the accumulation of uremic toxins. Since macrophage foam cell formation is a hallmark of atherosclerosis, we examined the direct effect of indoxyl sulfate (IS), a representative uremic toxin, on macrophage function. Macrophages differentiated from THP-1 cells were exposed to IS in vitro. IS decreased the cell viability of THP-1 derived macrophages but promoted the production of inflammatory cytokines (IL-1 beta, IS 1.0 mM: 101.8 +/- 21.8 pg/mL vs. 0 mM: 7.0 +/- 0.3 pg/mL, TNF-alpha, IS 1.0 mM: 96.6 +/- 11.0 pg/mL vs. 0 mM: 15.1 +/- 3.1 pg/mL) and reactive oxygen species. IS reduced macrophage cholesterol efflux (IS 0.5 mM: 30.3% +/- 7.3% vs. 0 mM: 43.5% +/- 1.6%) and decreased ATP-binding cassette transporter G1 expression. However, lipid uptake into cells was not enhanced. A liver X receptor (LXR) agonist, T0901317, improved IS-induced production of inflammatory cytokines as well as reduced cholesterol efflux. In conclusion, IS induced inflammatory reactions and reduced cholesterol efflux in macrophages. Both effects of IS were improved with activation of LXR. Direct interactions of uremic toxins with macrophages may be a major cause of atherosclerosis acceleration in patients with CKD.

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  • A case of membranoproliferative glomerulonephritis developed over twenty years with three different findings of renal pathology. 査読

    Kaneko Y, Yoshita K, Kabasawa H, Imai N, Ito Y, Ueno M, Nishi S, Narita I

    CEN case reports   2 ( 1 )   76 - 83   2013年5月

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    担当区分:筆頭著者, 責任著者  

    DOI: 10.1007/s13730-012-0042-1

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  • [Nephritis and nephrotic syndrome]. 査読

    Kaneko Y, Narita I

    Nihon Jinzo Gakkai shi   55 ( 1 )   35 - 41   2013年

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    担当区分:筆頭著者, 責任著者  

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  • Three cases of gastric antral vascular ectasia in chronic renal failure. 査読

    Iguchi A, James Kazama J, Komatsu M, Kaneko Y, Iino N, Goto S, Narita I

    Case reports in nephrology and urology   1 ( 1 )   15 - 19   2011年7月

  • [Membranoproliferative glomerulonephritis]. 査読

    Kaneko Y, Narita I

    Nihon Jinzo Gakkai shi   52 ( 7 )   899 - 902   2010年

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    担当区分:筆頭著者, 責任著者  

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  • Egg on the Table 査読

    Junichiro J. Kazama, Yoshikatsu Kaneko

    CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY   4 ( 1 )   14 - 15   2009年1月

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    記述言語:英語   出版者・発行元:AMER SOC NEPHROLOGY  

    DOI: 10.2215/CJN.05871108

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  • A case of nephrotic syndrome 11 yr post-kidney transplantation 査読

    S. Nishi, N. Imai, G. Nakamura, M. Ueno, K. Kawamura, Y. Kaneko, S. Goto, B. Alchi, K. Saito, K. Takahashi, F. Gejyo

    Clinical Transplantation   21 ( 18 )   31 - 35   2007年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We present here a male patient who had developed nephrotic syndrome 11 yr after kidney transplantation. Nephrotic syndrome suddenly occurred after severe sunburn he got in Hawaii. Such a clinical course seemed very rare in kidney transplantation, and multifactorial etiology was suspected. The histological findings of graft biopsy were intricate. On light microscopy, there were mesangial expansion and endocapillary proliferation with duplication of GBM and crescent formation. Deposits of IgM in mesangium were the most prominent finding on immunofluorescence microscopy, but IgA and C3 deposits were also detected. Electron microscopy revealed paramesangial and a few subepithelial dense deposits. Additionally, small organized deposits were found in the subepithelial space. Based on these findings, a provisional diagnosis of IgA nephropathy superimposed on chronic transplant glomerulopathy was made. However, hepatitis C virus related nephropathy could not be excluded. © 2007 Blackwell Munksgaard.

    DOI: 10.1111/j.1399-0012.2007.00715.x

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  • [Clinical nephrology]. 査読

    Kaneko Y, Narita I, Gejyo F

    Nihon Jinzo Gakkai shi   49 ( 1 )   19 - 24   2007年

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    担当区分:筆頭著者, 責任著者  

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  • Expression of allograft inflammatory factor-1 in kidneys: A novel molecular component of podocyte. 査読

    Tsubata Y, Sakatsume M, Ogawa A, Alchi B, Kaneko Y, Kuroda T, Kawachi H, Narita I, Yamamoto T, Gejyo F

    Kidney Int   70   1948 - 1954   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/sj.ki.5001941

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  • Bezafibrate suppresses rat antiglomerular basement membrane crescentic glomerulonephritis 査読

    D Saga, M Sakatsume, A Ogawa, Y Tsubata, Y Kaneko, T Kuroda, F Sato, J Ajiro, D Kondo, T Miida, Narita, I, F Gejyo

    KIDNEY INTERNATIONAL   67 ( 5 )   1821 - 1829   2005年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING INC  

    Background. The immunoregulatory activity of ligands for peroxisome proliferator-activated receptors (PPARs) has been recently paid attention. The regulatory effect of bezafibrate (BZF), a ligand for PPAR alpha on glomerulonephritis was investigated using a rat anti-glomerular basement membrane (GBM) glomerulonephritis model.
    Methods. The effect on development of anti-GBM glomerulonephritis was examined by treatment with BZF from day -7 to day 7 after intravenous injection of rabbit anti-GBM serum into Wistar Kyoto (WKY) rats. The therapeutic efficacy after onset of the glomerulonephritis was also checked by treatment with BZF from day 3 to 7. On day 7, the condition was evaluated histologically. The expression of a tissue injury molecule, macrophage metalloesterase (MME), was measured by Northern blot analysis. The suppressive effect on immune cells was assessed by proliferation assay with mitogen-stimulated rat spleen cells.
    Results. Histopathologic changes induced by anti-GBM in rats treated with BZF (day -7 to day 7) were markedly suppressed in a dose-dependent fashion. Infiltration of ED-1+ macrophages in glomeruli, proteinuria, and mRNA expression of MME in kidneys were diminished in parallel with histologic improvement. Moreover, the disease activity was also attenuated even by the treatment after onset of the glomerulonephritis (day 3 to 7). The mitogen-induced proliferation of spleen cells was down-regulated at concentrations of BZF, which were equivalent to those in sera of BZF-treated rats.
    Conclusion. BZF markedly suppresses the activity of rat anti-GBM crescentic glomerulonephritis. Fibrates might serve as a therapeutic option for crescentic glomerulonephritis.

    DOI: 10.1111/j.1523-1755.2005.00280.x

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  • Osteopontin expression in acute renal allograft rejection 査読

    B Alchi, S Nishi, D Kondo, Y Kaneko, A Matsuki, N Imai, M Ueno, S Iguchi, M Sakatsume, Narita, I, T Yamamoto, F Gejyo

    KIDNEY INTERNATIONAL   67 ( 3 )   886 - 896   2005年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING INC  

    Background. Osteopontin (OPN) is a potent chemoattractant for mononuclear cells that is up-regulated in various inflammatory states of the kidney. The role of OPN and its expression in human renal allograft rejection are unknown.
    Methods. We examined by immunohistochemistry and in situ hybridization, renal biopsies from patients with acute rejection (N = 22), protocol biopsies without rejection (N = 9), and perioperative donor biopsies (N = 35) for intrarenal expression of OPN, and its correlation with clinical, laboratory, and histopathologic parameters. In the rejection biopsies, interstitial monocyte/macrophage infiltration, tubulointerstitial cell proliferation/regeneration and apoptosis were investigated.
    Results. In the majority of rejection biopsies, OPN expression by proximal tubular epithelium was widespread, and tended to be enhanced in the tubules surrounded by numerous inflammatory cells. Conversely, in patients that did not experience episodes of rejection and in donor biopsies, OPN expression by proximal tubules was nil or weak. OPN mRNA was colocalized with its translated protein in the renal tubular epithelium. OPN expression positively correlated with the degree of interstitial inflammation (P &lt; 0.05), CD68+ monocyte infiltration (P &lt; 0.01), Ki-67+ regenerating tubular and interstitial cells (P &lt; 0.05 and P &lt; 0.005, respectively), but not with terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL)-positive apoptotic tubular cells.
    Conclusion. These data suggest that inducible expression of OPN in the tubular epithelium may have a pathogenic role in acute renal allograft rejection by mediating interstitial monocyte infiltration and possibly tubular regeneration.

    DOI: 10.1111/j.1523-1755.2005.00153.x

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  • Transforming growth factor-beta 1 gene polymorphism modifies the histological and clinical manifestations in Japanese patients with IgA nephropathy 査読

    F Sato, Narita, I, S Goto, D Kondo, N Saito, J Ajiro, D Saga, A Ogawa, M Kadomura, F Akiyama, Y Kaneko, M Ueno, M Sakatsume, F Gejyo

    TISSUE ANTIGENS   64 ( 1 )   35 - 42   2004年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Transforming growth factor (TGF)-beta1, a multifunctional cytokine, which regulates proliferation and differentiation of a variety of cell types, has the central role in the development and progression of renal injury in both animal models and human. Although it has been suggested that genetic variations in the TGF-beta1 gene are associated with the activity of the gene product, their clinical significance in glomerular disease is unknown. We investigated whether the polymorphisms of C-509T and T869C in TGF-beta1 account for interindividual variation in manifestations of IgA nephropathy (IgAN) using 626 Japanese subjects including 329 patients with histologically proven IgAN and 297 healthy controls with normal urinalysis. The frequencies of genotypes, alleles, and major haplotypes were similar between the patients and controls. The C-509T and T869C polymorphisms were in tight linkage disequilibrium, and the major haplotypes were C-C and T-T, which accounted for more than 95% of the total. In patients with -509CC and in those with the 869CC, urinary protein excretion was higher than in those with other genotypes, whereas no difference in other clinical manifestations was noted. Moreover, patients with -509CC and those with 869CC genotypes presented with a significant higher score of mesangial cell proliferation than in those with other genotypes. These results suggest that TGF-beta1 gene polymorphisms are specifically associated with heavy proteinuria and mesangial cell proliferation in Japanese patients with IgAN, although they do not confer susceptibility to this disease.

    DOI: 10.1111/j.1399-0039.2004.00256.x

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  • Impaired IFN-γ production of Vα24 NKT cells in non-remitting sarcoidosis. 査読

    Kobayashi S, Kaneko Y, Seino K, Yamada Y, Motohashi S, Koike J, Sugaya K, Kuriyama T, Asano S, Tsuda T, Wakao H, Harada M, Kojo S, Nakayama T, Taniguchi M

    International immunology   16 ( 2 )   215 - 222   2004年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/intimm/dxh020

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  • Progression of T cell lineage restriction in the earliest subpopulation of murine adult thymus visualized by the expression of lck proximal promoter activity 査読

    C Shimizu, H Kawamoto, M Yamashita, M Kimura, E Kondou, Y Kaneko, S Okada, T Tokuhisa, M Yokoyama, M Taniguchi, Y Katsura, T Nakayama

    INTERNATIONAL IMMUNOLOGY   13 ( 1 )   105 - 117   2001年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The proximal promoter of Ick directs gene expression exclusively in T cells. To investigate the developmental regulation of the Ick proximal promoter activity and its relationship to T cell lineage commitment, a green fluorescence protein (GFP) transgenic (Tg) mouse in which the GFP expression is under the control of the proximal promoter of Ick was created. In the adult GFP-Tg mice, &gt;90% of CD4(+)CD8(+) and CD4(+)CD8(-) thymocytes, and the majority of CD4(+)CD8(-) and CD4(-)CD8(-) [double-negative (DN)] thymocytes were highly positive for GFP, Slightly lower but substantial levels of expression of GFP was also observed in mature splenic T cells. No GFP(+) cells was detected in non-T lineage subsets, including mature and immature a cells, CD5(+) a cells, and NK cells, indicating a preserved tissue specificity of the promoter. The earliest GFP(+) cells detected were found in the CD44(+)CD25(-) DN thymocyte subpopulation, The developmental potential of GFP(-) and GFP(+) cells in the CD44(+)CD25(-) DN fraction was examined using in vitro culture systems. The generation of substantial numbers of ap and gamma delta T cells as well as NK cells was demonstrated from both GFP(-) and GFP(+) cells. However, no development of B cells or dendritic cells was detected from GFP(+) CD44(+)CD25(-) DN thymocytes. These results suggest that the progenitors expressing Ick proximal promoter activity in the CD44(+)CD25(-) DN thymocyte subset have lost most of the progenitor potential for the a and dendritic cell lineage, Thus, progression of T cell lineage restriction in the earliest thymic population can be visualized by Ick proximal promoter activity, suggesting a potential role of Lck in the T cell lineage commitment.

    DOI: 10.1093/intimm/13.1.105

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  • The role of alpha-galactosylceramide-activated V alpha 14 natural killer T cells in the regulation of Th2 cell differentiation 査読

    Nakayama T, Yamashita M, Kawano T, Shimizu C, Shibata Y, Kamata T, Kaneko Y, Kobayashi S, Takeda U, Motohashi S, Cui J. Q, Taniguchi M

    International Archives of Allergy and Immunology   124 ( 1-3 )   38 - 42   2001年

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MISC

  • 患者さんとご家族のためのCKD療養ガイド2018

    岡田 浩一, 安田 宜成, 柏原 直樹, 成田 一衛, 若杉三奈子, 金子 佳賢, 蒲澤 秀門

    患者さんとご家族のためのCKD療養ガイド2018   2018年12月

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  • 腎尿細管におけるホルモン再吸収機構

    金子佳賢, 斎藤亮彦

    月刊腎臓内科・泌尿器科   8 ( 2 )   172‐176 - 176   2018年8月

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    記述言語:日本語   出版者・発行元:科学評論社  

    CiNii Article

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  • エビデンスに基づくCKD診療ガイドライン2018

    成田 一衛, 若杉三奈子, 川村 和子, 金子 佳賢, 蒲澤 秀門, 細島 康宏, 岡田 浩一, 安田 宜成

    エビデンスに基づくCKD診療ガイドライン2018   2018年6月

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  • IgA腎症における口蓋扁桃局所での免疫応答と細菌群集の関連

    山口浩毅, 後藤眞, 土田雅史, 渡辺博文, 山本卓, 金子佳賢, 森宙史, 黒川顕, 成田一衞

    IgA腎症研究会学術集会抄録   41st   5   2018年

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    記述言語:日本語  

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  • 自記式食事歴質問票(DHQ)を用いた高齢CKD患者における食事性酸負荷の評価

    鳥羽 宏司, 細島 康宏, 蒲澤 秀門, 忰田 亮平, 渡邊 令子, 田邊 直仁, 金子 佳賢, 鈴木 芳樹, 成田 一衛, 斎藤 亮彦

    日本老年医学会雑誌   54 ( 4 )   634 - 634   2017年10月

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    記述言語:日本語   出版者・発行元:(一社)日本老年医学会  

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  • 高齢者における遺伝子組み換えヒトトロンボモジュリンの使用経験

    小泉 健, 近 幸吉, 田邊 嘉也, 井口 清太郎, 長谷川 隆志, 鈴木 栄一, 菊地 利明, 金子 佳賢, 成田 一衛

    日本老年医学会雑誌   54 ( 4 )   636 - 637   2017年10月

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    記述言語:日本語  

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  • インスリンに週1回GLP-1受容体作動薬デュラグルチドを併用し血糖変動を抑制できた経管栄養中高齢2型糖尿病患者の1例

    蒲澤 秀門, 土田 雅史, 飯田 倫理, 細島 康宏, 忰田 亮平, 保坂 聖子, 金子 佳賢, 鈴木 芳樹, 斎藤 亮彦, 成田 一衛

    日本老年医学会雑誌   54 ( 4 )   634 - 634   2017年10月

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    記述言語:日本語   出版者・発行元:(一社)日本老年医学会  

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  • 高齢者および若年者IgA腎症の病理組織所見と腎予後の比較

    金子佳賢, 吉田一浩, 河野恵美子, 伊藤由美, 今井直史, 酒巻裕一, 山本卓, 後藤眞, 成田一衛

    日本内科学会雑誌   105 ( Suppl. )   174 - 174   2016年2月

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    記述言語:日本語   出版者・発行元:(一社)日本内科学会  

    J-GLOBAL

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  • Klinefelter症候群が疑われ高度なインスリン抵抗性を示した糖尿病の1例

    矢田雄介, 細島康宏, 金子佳賢, 風間順一郎, 鈴木芳樹, 成田一衛, 斎藤亮彦

    新潟医学会雑誌   129 ( 8 )   481   2015年8月

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    記述言語:日本語  

    J-GLOBAL

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  • RENAL DIFFERETIATION OF PATIENT SPECIFIC HUMAN INDUCED PLURIPOTENT STEM CELLS; A NOVEL APPROACH TO STUDY MECHANISMS OF RENAL COLOBOMA SYNDROME

    Otsuka Tadashi, Koyama Kyuutaro, Kaneko Yoshikatsu, Narita Ichiei

    NEPHROLOGY   19   84 - 85   2014年5月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:WILEY-BLACKWELL  

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  • 抗リン脂質抗体症候群,Budd‐Chiari症候群に続発し,意識障害・腎障害を示した1例

    山本卓, 金子佳賢, 成田一衛

    臨床透析   29 ( 12 )   1767 - 1773   2013年11月

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    記述言語:日本語   出版者・発行元:(株)日本メディカルセンター  

    DOI: 10.19020/J01864.2014064176

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  • シタグリプチンとグリメピリドの隔日内服中にCGMにより血糖変動を確認できた1例

    石川友美, 細島康宏, 吉田一浩, 金子佳賢, 風間順一郎, 鈴木芳樹, 成田一衛, 斎藤亮彦

    糖尿病   56 ( 7 )   447   2013年7月

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    記述言語:日本語  

    J-GLOBAL

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  • 心不全モデルマウスにおける近位尿細管プロラクチン受容体の発現増加

    土田陽平, 金子佳賢, 大塚忠司, 後藤慧, 斎藤亮彦, 山本格, 成田一衛

    日本腎臓学会誌   55 ( 3 )   314   2013年4月

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    記述言語:日本語  

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  • DHQを用いた慢性腎臓病患者における摂取たんぱく質の種類と量の検討

    細島康宏, 山本卓, 金子佳賢, 後藤眞, 村松芳多子, 渡邊令子, 成田一衛, 鈴木芳樹, 斎藤亮彦

    日本腎臓学会誌   55 ( 3 )   420   2013年4月

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    記述言語:日本語  

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  • 市民公開CKDセミナーの開催と成果

    小出真希子, 金子佳賢, 山本卓, 原正則, 島田久基, 菊地博, 近藤大介, 小柳やよい, 成田一衛, 丸山弘樹

    日本透析医学会雑誌   45 ( Supplement 1 )   670 - 670   2012年5月

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    記述言語:日本語   出版者・発行元:(一社)日本透析医学会  

    J-GLOBAL

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  • 心腎症候群モデルマウスと関連遺伝子の検索

    土田陽平, 金子佳賢, 斎藤亮彦, 山本格, 成田一衛

    日本腎臓学会誌   53 ( 3 )   464   2011年5月

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    記述言語:日本語  

    J-GLOBAL

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  • The genetic susceptibility to IgA nephropathy: A novel functional candidate gene for incomplete O-glycosylation of IgA1

    I. Narita, Y. Kaneko, D. Kondo, S. Goto, M. Sakatsume, F. Gejyo

    KIDNEY INTERNATIONAL   71 ( 5 )   379 - 381   2007年3月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Incompleteness of O-glycosylation in the IgA1 hinge has been implicated as a central mechanism in the development of IgA nephropathy. Although underglycosylation was reported to be an acquired abnormality, genes for enzymes of O-glycosylation, such as C1GALT1, may be responsible for susceptibility to IgA nephropathy.

    DOI: 10.1038/sj.ki.5002139

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  • THE ANALYSIS OF SM22 alpha EXPRESSION IN RAT ANTI-GLOMERULAR BASEMENT MEMBRANE NEPHRITIS

    Asa Ogawa, Minoru Sakatsume, Daisuke Saga, Yutaka Tsubata, Yoshikatsu Kaneko, Ichiei Narita, Fumitake Gejyo

    NEPHROLOGY   10   A41 - A41   2005年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:WILEY-BLACKWELL  

    Web of Science

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  • L-Selectin(CD62L) in urine: As a marker of cell-mediated inflammation in kidneys

    M Sakatsume, D Saga, Y Kaneko, F Sato, J Ajiro, D Kondo, Narita, I, F Gejyo

    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY   14   150A - 150A   2003年11月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Web of Science

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  • サルコイドーシスにおけるNKT細胞の関与

    山田 嘉仁, 金子 佳賢, 小林 誠一郎, 巽 浩一郎, 山口 哲生, 栗山 喬之, 中山 俊憲, 谷口 克

    日本呼吸器学会雑誌   39 ( 増刊 )   262 - 262   2001年3月

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    記述言語:日本語   出版者・発行元:(一社)日本呼吸器学会  

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  • ConA誘導性肝炎におけるVα14NKT細胞の役割

    金子佳賢, 原田通成, 河野鉄, 柴田陽一, 山下政克, 中山俊憲, 谷口克

    日本免疫学会総会・学術集会記録   29   59   1999年10月

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    記述言語:日本語  

    J-GLOBAL

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産業財産権

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受賞

  • 平成27年度学術奨励賞

    2015年   新潟県医師会   免疫学的アプローチによる腎炎発症の分子生化学的機序の解明と治療応用

    金子佳賢

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  • International Immunology Outstanding Merit Award 2012

    2013年   日本免疫学会   Integrin α1/β1 and α2/β1 as a receptor for IgA1 in human glomerular mesangial cells in IgA nephropathy.

    金子佳賢

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  • 第4回腎疾患と高血圧研究会奨励賞

    2012年   腎疾患と高血圧研究会   心腎連関モデルマウスにおける近位尿細管でのプロラクチン受容体発現上昇とその役割

    金子佳賢

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共同研究・競争的資金等の研究

  • ループス腎炎の発症病態における抗リボソームP抗体の関与

    2018年4月 - 2021年3月

    日本学術振興会  科学研究費 基盤研究 (C) 

    佐藤弘惠

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    資金種別:競争的資金

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  • 腎糸球体メサンギウム細胞とIgA1の相互作用および関連分子による修飾機構

    2016年4月 - 2019年3月

    日本学術振興会  科学研究費 基盤研究 (C) 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • 腎臓病モデルマウスにおける加齢の影響とプロラクチンの尿蛋白減少作用

    2015年4月 - 2016年3月

    ノバルティスファーマ  ノバルティス老化および老年医学研究基金研究助成 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • ホロゲノム解析によるIgA腎症の病態解析と治療ターゲット探索

    2014年4月 - 2018年3月

    日本学術振興会  科学研究費 基盤研究 (B) 

    成田一衛

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    資金種別:競争的資金

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  • 腎臓病患者におけるiPS細胞由来腎糸球体上皮細胞の機能解析と病態解明

    2014年4月 - 2017年3月

    日本学術振興会  科学研究費 挑戦的萌芽研究 

    成田一衛

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    資金種別:競争的資金

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  • 肥満ホルモンと免疫細胞の相互作用による腎疾患への関与

    2014年4月 - 2015年3月

    鈴木謙三記念医科学応用研究財団  平成26年度調査研究助成金 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • 腎糸球体上皮細胞におけるプロラクチン受容体の腎疾患における役割の解明

    2012年4月 - 2015年3月

    日本学術振興会  科学研究費 基盤研究 (C) 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • IgA腎症におけるメサンギウム細胞へのIgA沈着メカニズムの解明と治療への応用

    2009年4月 - 2012年3月

    日本学術振興会  科学研究費 若手研究 (B) 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • 糖鎖不全IgAに対する新規受容体の同定と解析によるIgA腎症発症機序の解明

    2008年4月 - 2011年3月

    日本学術振興会  科学研究費 基盤研究 (B) 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • 糖鎖不全IgAに対するメサンギウム細胞新規受容体の同定とIgA腎症発症メカニズムの解明

    2008年4月 - 2009年3月

    かなえ医薬振興財団  第37回かなえ医薬振興財団研究助成金 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • ガラクトース欠損IgAと結合するメサンギウム細胞新規受容体の同定

    2007年4月 - 2008年3月

    IgA腎症研究会  2007年度IgA腎症研究会研究助成金 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • 免疫グロブリンによる免疫抑制機序の解明

    2005年4月 - 2006年3月

    上原記念生命科学財団  平成16年度上原記念生命科学財団海外留学助成リサーチフェローシップ 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • マクロファージ抑制性レセプターFcγRIIBの発現調節機構の解明と疾患モデルへの臨床応用

    2004年4月 - 2005年3月

    内藤記念科学振興財団  第20回内藤記念科学振興財団海外研究留学助成金 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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  • マクロファージ抑制性レセプターFcγRIIBの発現調節機構の解明と疾患モデルへの臨床応用

    2004年4月 - 2005年3月

    かなえ医薬振興財団  第32回かなえ医薬振興財団海外留学助成金 

    金子佳賢

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    担当区分:研究代表者  資金種別:競争的資金

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担当経験のある授業科目

  • 内科学2

    2011年
    -
    現在
    機関名:新潟大学