2025/10/14 更新

写真a

ナガシマ ヒロシ
長島 寛
NAGASHIMA Hiroshi
所属
教育研究院 医歯学系 医学系列 准教授
医歯学総合研究科 生体機能調節医学専攻 機能再建医学 准教授
職名
准教授
外部リンク

学位

  • 博士(理学) ( 2005年3月   神戸大学 )

研究キーワード

  • 形態学

  • Embryology

  • 進化発生学

  • 実験発生学

研究分野

  • ライフサイエンス / 解剖学

経歴(researchmap)

  • 新潟大学大学院   医歯学総合研究科 肉眼解剖学分野   准教授

    2015年1月 - 現在

      詳細を見る

  • 新潟大学大学院   医歯学総合研究科, 肉眼解剖学分野   助教

    2011年4月 - 2014年12月

      詳細を見る

  • 独立行政法人 理化学研究所, 発生・再生科学総合研究センター   形態進化研究グループ   研究員

    2006年4月 - 2011年3月

      詳細を見る

  • 独立行政法人 理化学研究所, 発生・再生科学総合研究センター   形態進化研究グループ   テクニカルスタッフ

    2005年4月 - 2006年3月

      詳細を見る

経歴

  • 新潟大学   医歯学総合研究科 生体機能調節医学専攻 機能再建医学   准教授

    2015年1月 - 現在

  • 新潟大学   医学部 医学科   助教

    2011年4月 - 2014年12月

  • 新潟大学   医歯学総合研究科 生体機能調節医学専攻 機能再建医学   助教

    2011年4月 - 2014年12月

所属学協会

 

論文

  • Chondrocranial variation in chicken domestication

    Daniel Núñez‐León, Hiroshi Nagashima, Marcelo R. Sánchez‐Villagra

    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution   338 ( 8 )   505 - 515   2022年10月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Abstract

    The chondrocranium is a key structure of the skull, but our knowledge of its embryonic development is based mostly on investigations of few stages across taxa. Variation of chondrocranial features is known across species, but little is known about intraspecific variation, or its evolution in the context of domestication. Here, we investigated two specific structures of the chondrocranium in three windows of embryonic development. The anatomy of one of these structures was also compared among adult skulls of chickens and their wild ancestor (red junglefowl [RJF]). The proccesus tectalis and the prenasal process, along with the surrounding area of the orbitonasal foramina, presented variation throughout the ontogeny and in the adults. The processus tectalis showed distinct variation from the earliest stage studied to the adult. The numbers of orbitonasal foramina were also found to be variable in the ancestor and breeds studied. Furthermore, during early embryonic development, the prenasal process is similar across breeds and RJF, but later in embryonic development this structure presents variable states. The embryonic and adult variation found herein could be an example of intraspecific variation under domestication, resulting from different types of tissue interrelationship during development.

    DOI: 10.1002/jez.b.23177

    researchmap

    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jez.b.23177

  • Skeletal variation in bird domestication: limb proportions and sternum in chicken, with comparisons to mallard ducks and Muscovy ducks

    Carlos Manuel Herrera-Castillo, Madeleine Geiger, Daniel Núñez-León, Hiroshi Nagashima, Sabine Gebhardt-Henrich, Michael Toscano, Marcelo R. Sanchez-Villagra

    PeerJ   10   e13229 - e13229   2022年4月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:PeerJ  

    Background

    Domestication, including selective breeding, can lead to morphological changes of biomechanical relevance. In birds, limb proportions and sternum characteristics are of great importance and have been studied in the past for their relation with flight, terrestrial locomotion and animal welfare. In this work we studied the effects of domestication and breed formation in limb proportions and sternum characteristics in chicken (Gallus gallus), mallard ducks (Anas plathyrhynchos) and Muscovy ducks (Cairina moschata).

    Methods

    First, we quantified the proportional length of three long bones of the forelimb (humerus, radius, and carpometacarpus) and the hind limb (femur, tibiotarsus, and tarsometatarsus) in domestic chickens, mallard ducks, and Muscovy ducks and their wild counterparts. For this, we took linear measurements of these bones and compared their proportions in the wildvs.the domestic group in each species. In chicken, these comparisons could also be conducted among different breeds. We then evaluated the proportional differences in the context of static and ontogenetic allometry. Further, we compared discrete sternum characteristics in red jungle fowl and chicken breeds. In total, we examined limb bones of 287 specimens and keel bones of 63 specimens.

    Results

    We found a lack of significant change in the proportions of limb bones of chicken and Muscovy duck due to domestication, but significant differences in the case of mallard ducks. Variation of evolvability, allometric scaling, and heterochrony may serve to describe some of the patterns of change we report. Flight capacity loss in mallard ducks resulting from domestication may have a relation with the difference in limb proportions. The lack of variation in proportions that could distinguish domestic from wild forms of chicken and Muscovy ducks may reflect no selection for flight capacity during the domestication process in these groups. In chicken, some of the differences identified in the traits discussed are breed-dependent. The study of the sternum revealed that the condition of crooked keel was not unique to domestic chicken, that some sternal characteristics were more frequent in certain chicken breeds than in others, and that overall there were no keel characteristics that are unique for certain chicken breeds. Despite some similar morphological changes identified across species, this study highlights the lack of universal patterns in domestication and breed formation.

    DOI: 10.7717/peerj.13229

    researchmap

    その他リンク: https://peerj.com/articles/13229.xml

  • Development of fin‐innervating motor neurons after peripheral target removal in medaka fish

    Akina Chiba, Kenichi Soma, Keisuke Watanabe, Hiroshi Nagashima, Noboru Sato

    Developmental Neurobiology   81 ( 2 )   110 - 122   2020年12月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Abstract

    Peripheral targets regulate the development and survival of the nerve centers that serve them, because the elimination of the target normally results in massive death of the developing neurons that innervate it. This widely accepted theory appears to be well supported by developing limbs and their innervation in tetrapods, but it is unclear whether this concept applies to primitive vertebrates that have paired appendages. In this study, we examined the development of spinal motor neurons following pectoral fin bud removal (FBR) in medaka fish. After FBR, motor axons initially extended to the plexus region in a morphologically normal pattern. During the period of fin innervation, motor axons in the FBR‐medaka failed to form the normal brachial plexus and elongated ventrally toward the abdominal region. In the ventral horn that would normally innervate the pectoral fin, however, neurons did not undergo cell death following FBR. There were no differences in the numbers of axons in the ventral roots between the FBR and control sides. Motor neuron markers, RALDH2 and FOXP1, that are expressed in limb‐innervating motor neurons in the lateral motor column in tetrapods, were also expressed in the ventral horns of both the control and FBR sides in medaka fish. These results suggest that, although both tetrapod and medaka motor neurons share the same molecular characteristics for innervating paired appendages, the fates of neurons differ following the removal of their peripheral target. Therefore, the relationship between the peripheral target and its nerve center may be altered among vertebrates.

    DOI: 10.1002/dneu.22799

    researchmap

    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/dneu.22799

  • Novel concept for the epaxial/hypaxial boundary based on neuronal development 査読 国際誌

    Hiroshi Nagashima, Daisuke Koga, Satoshi Kusumi, Katsuki Mukaigasa, Hiroyuki Yaginuma, Tatsuo Ushiki, Noboru Sato

    Journal of Anatomy   237 ( 3 )   427 - 438   2020年8月

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Trunk muscles in vertebrates are classified as either dorsal epaxial or ventral hypaxial muscles. Epaxial and hypaxial muscles are defined as muscles innervated by the dorsal and ventral rami of spinal nerves, respectively. Each cluster of spinal motor neurons passing through dorsal rami innervates epaxial muscles, whereas clusters traveling on the ventral rami innervate hypaxial muscles. Herein, we show that some motor neurons exhibiting molecular profiles for epaxial muscles follow a path in the ventral rami. Dorsal deep-shoulder muscles and some body wall muscles are defined as hypaxial due to innervation via the ventral rami, but a part of these ventral rami has the molecular profile of motor neurons that innervate epaxial muscles. Thus, the epaxial and hypaxial boundary cannot be determined simply by the ramification pattern of spinal nerves. We propose that, although muscle innervation occurs via the ventral rami, dorsal deep-shoulder muscles and some body wall muscles represent an intermediate group that lies between epaxial and hypaxial muscles.

    DOI: 10.1111/joa.13219

    PubMed

    researchmap

    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/joa.13219

  • Morphological diversity of integumentary traits in fowl domestication: Insights from disparity analysis and embryonic development

    Daniel Núñez‐León, Gabriel Aguirre‐Fernández, Andrea Steiner, Hiroshi Nagashima, Per Jensen, Esther Stoeckli, Richard A. Schneider, Marcelo R. Sánchez‐Villagra

    Developmental Dynamics   248 ( 11 )   1044 - 1058   2019年9月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Abstract

    The domestication of the fowl resulted in a large diversity of integumental structures in chicken breeds. Several integumental traits have been investigated from a developmental genetics perspective. However, their distribution among breeds and their developmental morphology remain unexplored. We constructed a discrete trait‐breed matrix and conducted a disparity analysis to investigate the variation of these structures in chicken breeds; 20 integumental traits of 72 chicken breeds and the red junglefowl were assessed. The analyses resulted in slight groupings of breed types comparable to standard breed classification based on artificial selection and chicken type use. The red junglefowl groups together with bantams and European breeds. We provide new data on the red junglefowl and four chicken breeds, demonstrating where and when variation arises during embryonic development. We document variation in developmental timing of the egg tooth and feather formation, as well as other kinds of developmental patterning as in the anlagen of different type of combs. Changes in epithelial‐mesenchymal signaling interactions may drive the highly diverse integument in chickens. Experimental and comparative work has revealed that the cranial neural crest mesenchyme mediates its interactions with the overlying epithelium and is the likely source of patterning that generates diversity in integumental structures.

    DOI: 10.1002/dvdy.105

    researchmap

    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/dvdy.105

  • Development of the chondrocranium in the domesticated fowl (Gallus gallus f. domestica), with a study on the variation of the of hypoglossal foramina. 査読

    Evelyn Hüppi, Daniel Núñez-León, Hiroshi Nagashima, Marcelo R. Sánchez-Villagra

    Vertebrate Zoology   69   299 - 310   2019年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    researchmap

  • Developmental origin of the clavicle, and its implications for the evolution of the neck and the paired appendages in vertebrates 査読

    Hiroshi Nagashima, Fumiaki Sugahara, Keisuke Watanabe, Masahiro Shibata, Akina Chiba, Noboru Sato

    JOURNAL OF ANATOMY   229 ( 4 )   536 - 548   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/joa.12502

    Web of Science

    PubMed

    researchmap

  • On the homology of the shoulder girdle in turtles. 査読

    Nagashima, H, Sugahara, F, Takechi, M, Sato, N, Kuratani, S

    J. Exp. Zool. B Mol. Dev. Evol.   324 ( 3 )   244 - 254   2015年5月

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The shoulder girdle in turtles is encapsulated in the shell and has a triradiate morphology. Due to its unique configuration among amniotes, many theories have been proposed about the skeletal identities of the projections for the past two centuries. Although the dorsal ramus represents the scapular blade, the ventral two rami remain uncertain. In particular, the ventrorostral process has been compared to a clavicle, an acromion, and a procoracoid based on its morphology, its connectivity to the rest of the skeleton and to muscles, as well as with its ossification center, cell lineage, and gene expression. In making these comparisons, the shoulder girdle skeleton of anurans has often been used as a reference. This review traces the history of the debate on the homology of the shoulder girdle in turtles. And based on the integrative aspects of developmental biology, comparative morphology, and paleontology, we suggest acromion and procoracoid identities for the two ventral processes.

    DOI: 10.1002/jez.b.22584

    PubMed

    researchmap

  • On the Homology of the Shoulder Girdle in Turtles 査読

    Hiroshi Nagashima, Fumiaki Sugahara, Masaki Takechi, Noboru Sato, Shigeru Kuratani

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION   324 ( 3 )   244 - 254   2015年5月

     詳細を見る

  • Comparative study of the shell development of hard- and soft-shelled turtles 査読

    Hiroshi Nagashima, Masahiro Shibata, Mari Taniguchi, Shintaro Ueno, Naoki Kamezaki, Noboru Sato

    JOURNAL OF ANATOMY   225 ( 1 )   60 - 70   2014年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/joa.12189

    Web of Science

    PubMed

    researchmap

  • Origin of the unique morphology of the shoulder girdle in turtles 査読

    Hiroshi Nagashima, Tatsuya Hirasawa, Fumiaki Sugahara, Masaki Takechi, Ryo Usuda, Noboru Sato, Shigeru Kuratani

    JOURNAL OF ANATOMY   223 ( 6 )   547 - 556   2013年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/joa.12116

    Web of Science

    PubMed

    researchmap

  • The turtle evolution: A conundrum in vertebrate evo-devo 招待 査読

    Naoki Irie, Hiroshi Nagashima, Shigeru Kuratani

    New Principles in Developmental Processes   303 - 314   2013年11月

     詳細を見る

    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer Japan  

    DOI: 10.1007/978-4-431-54634-4_23

    Scopus

    researchmap

  • The endoskeletal origin of the turtle carapace 査読

    Tatsuya Hirasawa, Hiroshi Nagashima, Shigeru Kuratani

    NATURE COMMUNICATIONS   4   2107   2013年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/ncomms3107

    Web of Science

    PubMed

    researchmap

  • Origin of the turtle body plan: The folding theory to illustrate turtle-specific developmental repatterning 招待 査読

    Hiroshi Nagashima, Shigehiro Kuraku, Katsuhisa Uchida, Yoshie Kawashima-Ohya, Yuichi Narita, Shigeru Kuratani

    Vertebrate Paleobiology and Paleoanthropology   ( 9789400743083 )   37 - 50   2013年

     詳細を見る

    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer  

    DOI: 10.1007/978-94-007-4309-0_4

    Scopus

    researchmap

  • Body plan of turtles: an anatomical, developmental and evolutionary perspective. 査読

    Hiroshi Nagashima, Shigehiro Kuraku, Katsuhisa Uchida, Yoshie Kawashima-Ohya, Yuichi Narita, Shigeru Kuratani

    Anatomical science international   87 ( 1 )   1 - 13   2012年3月

     詳細を見る

    記述言語:英語  

    The evolution of the turtle shell has long been one of the central debates in comparative anatomy. The turtle shell consists of dorsal and ventral parts: the carapace and plastron, respectively. The basic structure of the carapace comprises vertebrae and ribs. The pectoral girdle of turtles sits inside the carapace or the rib cage, in striking contrast to the body plan of other tetrapods. Due to this topological change in the arrangement of skeletal elements, the carapace has been regarded as an example of evolutionary novelty that violates the ancestral body plan of tetrapods. Comparing the spatial relationships of anatomical structures in the embryos of turtles and other amniotes, we have shown that the topology of the musculoskeletal system is largely conserved even in turtles. The positional changes seen in the ribs and pectoral girdle can be ascribed to turtle-specific folding of the lateral body wall in the late developmental stages. Whereas the ribs of other amniotes grow from the axial domain to the lateral body wall, turtle ribs remain arrested axially. Marginal growth of the axial domain in turtle embryos brings the morphologically short ribs in to cover the scapula dorsocaudally. This concentric growth appears to be induced by the margin of the carapace, which involves an ancestral gene expression cascade in a new location. These comparative developmental data allow us to hypothesize the gradual evolution of turtles, which is consistent with the recent finding of a transitional fossil animal, Odontochelys, which did not have the carapace but already possessed the plastron.

    DOI: 10.1007/s12565-011-0121-y

    PubMed

    researchmap

  • Hepatocyte growth factor is crucial for development of the carapace in turtles 査読

    Yoshie Kawashima-Ohya, Yuichi Narita, Hiroshi Nagashima, Ryo Usuda, Shigeru Kuratani

    EVOLUTION & DEVELOPMENT   13 ( 3 )   260 - 268   2011年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1525-142X.2011.00474.x

    Web of Science

    PubMed

    researchmap

  • Evolutionary developmental perspective for the origin of turtles: the folding theory for the shell based on the developmental nature of the carapacial ridge 査読

    Kuratani, Shigeru, Kuraku, Shigehiro, Nagashima, Hiroshi

    Evolution & Development   13 ( 1 )   1 - 14   2011年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1525-142X.2010.00451.x

    Web of Science

    PubMed

    researchmap

  • Skeletal Development in the Chinese Soft-Shelled Turtle Pelodiscus sinensis (Testudines: Trionychidae) 査読

    Marcelo R. Sanchez-Villagra, Hendrik Mueller, Christopher A. Sheil, Torsten M. Scheyer, Hiroshi Nagashima, Shigeru Kuratani

    JOURNAL OF MORPHOLOGY   270 ( 11 )   1381 - 1399   2009年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/jmor.10766

    Web of Science

    researchmap

  • Evolution of the Turtle Body Plan by the Folding and Creation of New Muscle Connections 査読

    Hiroshi Nagashima, Fumiaki Sugahara, Masaki Takechi, Rolf Ericsson, Yoshie Kawashima-Ohya, Yuichi Narita, Shigeru Kuratani

    SCIENCE   325 ( 5937 )   193 - 196   2009年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1126/science.1173826

    Web of Science

    PubMed

    researchmap

  • A developmental basis for innovative evolution of the turtle shell 招待 査読

    Shigeru Kuratani, Hiroshi Nagashima

    From Clone to Bone: The Synergy of Morphological and Molecular Tools in Palaeobiology   279 - 300   2009年1月

     詳細を見る

    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Cambridge University Press  

    DOI: 10.1017/CBO9780511760174.011

    Scopus

    researchmap

  • On the carapacial ridge in turtle embryos: its developmental origin, function and the chelonian body plan. 査読 国際誌

    Hiroshi Nagashima, Shigehiro Kuraku, Katsuhisa Uchida, Yoshie Kawashima Ohya, Yuichi Narita, Shigeru Kuratani

    Development (Cambridge, England)   134 ( 12 )   2219 - 26   2007年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The chelonian carapace is composed of dorsolaterally expanded ribs; an evolutionary change in the rib-patterning program is assumed to be related to this novelty. Turtle embryos exhibit a longitudinal ridge called the carapacial ridge (CR) on the flank, and its histological resemblance to the apical ectodermal ridge of the limb bud implies its inductive activity in the unique patterning of the ribs. We studied the Chinese soft-shelled turtle, Pelodiscus sinensis, and confirmed by labeling with a lipophilic dye, DiI, that the CR contains the somite-derived dermis and that it is a unique structure among amniotes. Using electroporation of a dominant-negative form of LEF-1, the CR-specific gene, we showed that CR-specific genes function in the growth and maintenance of the CR. Microcauterization or implantation of the CR did not change the dorsoventral pattern of the ribs, and only their fan-shaped pattern was arrested by CR removal. We conclude that the CR is a true embryonic novelty among amniotes and, because of the specific expression of regulatory genes, it functions in the marginal growth of the carapacial primordium, thereby inducing the fan-shaped arrangement of the ribs.

    DOI: 10.1242/dev.002618

    PubMed

    researchmap

  • Autopodial development in the sea turtles Chelonia mydas and Caretta caretta 査読

    Marcelo R. Sanchez-Villagra, Christian Mitgutsch, Hiroshi Nagashima, Shigeru Kuratani

    ZOOLOGICAL SCIENCE   24 ( 3 )   257 - 263   2007年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2108/zsj.24.257

    Web of Science

    researchmap

  • Developmental background for the origin of turtles 査読

    Kuratani, Shigeru, Kuraku, Shigehiro, Nagashima, Hiroshi

    Journal of Morphology   268 ( 12 )   1096 - 1096   2007年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    Web of Science

    researchmap

  • Unique features of Myf-5 in turtles: nucleotide deletion, alternative splicing, and unusual expression pattern 査読

    Kawashima Ohya, Yoshie, Usuda, Ryo, Kuraku, Shigehiro, Nagashima, Hiroshi, Kuratani, Shigeru

    Evolution & Development   8 ( 5 )   415 - 423   2006年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1525-142X.2006.00115.x

    Web of Science

    researchmap

  • Turtle-chicken chimera: an experimental approach to understanding evolutionary innovation in the turtle. 査読 国際誌

    Hiroshi Nagashima, Katsuhisa Uchida, Keiko Yamamoto, Shigehiro Kuraku, Ryo Usuda, Shigeru Kuratani

    Developmental dynamics : an official publication of the American Association of Anatomists   232 ( 1 )   149 - 61   2005年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Turtles have a body plan unique among vertebrates in that their ribs have shifted topographically to a superficial layer of the body and the trunk muscles are greatly reduced. Identifying the developmental factors that cause this pattern would further our understanding of the evolutionary origin of the turtles. As the first step in addressing this question, we replaced newly developed epithelial somites of the chicken at the thoracic level with those of the Chinese soft-shelled turtle Pelodiscus sinensis (P. sinensis somites into a chicken host) and observed the developmental patterning of the grafted somites in the chimera. The P. sinensis somites differentiated normally in the chicken embryonic environment into sclerotomes and dermomyotomes, and the myotomes differentiated further into the epaxial and hypaxial muscles with histological morphology similar to that of normal P. sinensis embryos and not to that of the chicken. Epaxial dermis also arose from the graft. Skeletal components, however, did not differentiate from the P. sinensis sclerotome, except for small fragments of cartilage associated with the host centrum and neural arches. We conclude that chicken and P. sinensis share the developmental programs necessary for the early differentiation of somites and that turtle-specific traits in muscle patterning arise mainly through a cell-autonomous developmental process in the somites per se. However, the mechanism for turtle-specific cartilage patterning, including that of the ribs, is not supported by the chicken embryonic environment.

    DOI: 10.1002/dvdy.20235

    PubMed

    researchmap

  • Poly Aromatic hydrocarbon concentration and 7-ethoxyresorufin O-deethylase activity in environment. 査読

    Nagashima H, Ono Y, Sekiguchi M

    Environmental Sciences   10   37 - 50   2003年

     詳細を見る

    記述言語:英語  

    researchmap

  • Evaluation of aquatic environment by polyaromatic hydrocarbon concentration and induction of EROD activity in HepG2 cells 査読

    H Nagashima, Y Ono, M Sekiguchi

    CHEMISTRY LETTERS   31 ( 10 )   1070 - 1071   2002年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1246/cl.2002.1070

    Web of Science

    researchmap

  • 最終処分場浸出水へのバイオテストの適用とモニタリング評価. 査読

    毛利紫乃, 長島 寛, 宮原康明, 小野芳朗, 河原長美, 国本 学, 宗宮 功

    廃棄物学会論文誌   13 ( 5 )   289 - 297   2002年

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Society of Material Cycles and Waste Management  

    廃棄物最終処分場が周辺水環境に与える影響の評価を行うため, 本研究では3種のバイオアッセイ (遺伝子毒性試験, ヒト乳癌由来細胞 (MCF-7) 増殖活性試験, 培養細胞毒性試験) を用い, 6ヶ月間にわたって廃棄物処分場試料, 周辺環境水の毒性監視を行った。また, 遺伝子毒性試験<I>umu</I>試験について環境監視に適した試料前処理法と新たな毒性単位を検討, 提唱した。その結果, 各試料の遺伝子毒性に季節特異的な変動は見られず, 浸出水に検出された毒性は生物処理によって周辺環境水程度に低減していた。浸出水のMCF-7増殖活性の平均値は低く, 放流先河川への影響はほとんどないと考えられた。細胞毒性試験において濃縮試料も細胞の生存率を50%以上低下させる毒性を示さず, 濃縮試料で検出された細胞毒性も, その由来は浸透圧であると推測された。この処理後放流水の細胞毒性の最大値は放流先上流の約9倍であり, 放流先河川に全く影響を与えないためには, 10倍の希釈が必要であった。

    DOI: 10.3985/jswme.13.289

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: https://jlc.jst.go.jp/DN/JALC/00387603535?from=CiNii

  • バイオアッセイによる排水試料の有害性評価に伴う問題点の解析. 査読

    長島 寛, 藤原朋広, 国本 学

    水環境学会誌   24 ( 2 )   110 - 114   2001年

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Society on Water Environment  

    To evaluate the toxicity existing in effluent water, three different bioassays, <i>in vitro</i> cytotoxicity tests using three human cell lines, bioluminescene inhibition test using luminescent bacteria and daphnia acute immobilization test, were applied to effluent water samples collected from solid-waste incineration plants. Results obtained from bioassays were compared with the results from chemical and physical analysis.<br>Toxicity observed with bioassays were not necessarily correlated with individual indices from chemical analysis, such as AOX (adsorbable organic halides), dioxins, heavy metal contents and pH. However, the toxicity to human cell lines and daphnia were well correlated with the osmolarity of the samples. Moreover the dose-response curves coincided well with those of standard osmolarity solutions made of sodium chloride or glucose, suggesting that the osmolarity plays a significant role in the toxic effects of these effluent water samples.<br>Therefore, for the application of bioassays to effluent water samples, especially in case of <i>in vitro</i> cytotoxicity tests and daphnia test, consideration of the osmolarity of the samples is essential for accurate assessment of their toxicity.

    DOI: 10.2965/jswe.24.110

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: https://jlc.jst.go.jp/DN/JALC/00090034565?from=CiNii

  • ヒト由来培養細胞系を用いた簡易バイオアッセイの高感度化と湖水・河川水への適用 査読

    藤原朋広, 長島 寛, 杉浦則夫, 国本 学

    水環境学会誌   24   63 - 58   2001年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2965/jswe.24.58

    researchmap

▶ 全件表示

書籍等出版物

  • このは, 8巻, 52-53

    長島 寛( 範囲: カメの不思議な体)

    文一総合出版  2014年 

     詳細を見る

  • 進化学事典

    長島 寛( 担当: 分担執筆 ,  範囲: 12.3 甲羅の発生とカメの起源)

    共立出版  2012年 

     詳細を見る

  • 遺伝. 64巻2号, 35-40頁

    長島 寛, 倉谷 滋( 範囲: カメはどうやって甲羅を作ったのか?)

    エヌ•ティー•エス  2010年 

     詳細を見る

  • 科学. 79巻11号, 1177-1180頁

    倉谷 滋, 長島 寛( 担当: 分担執筆 ,  範囲: カメの謎 甲羅はどのようにして獲得されたのか)

    岩波書店  2009年 

     詳細を見る

MISC

受賞

  • Anatomical Society Journal of Anatomy Best Paper Prize 2020

    2021年5月  

     詳細を見る

  • Journal of Anatomy Best Paper Prize 2016

    2017年5月   Anatomical Society  

    長島 寛

     詳細を見る

  • Journal of Anatomy Runner-Up Best Paper Prize 2013

    2014年5月   Anatomical Society, UK  

    長島 寛

     詳細を見る

  • 奨励賞

    2011年3月   日本解剖学会  

    長島 寛

     詳細を見る

  • 理化学研究所 研究奨励賞

    2011年3月  

     詳細を見る

  • 第147回学術集会 大会長賞

    2009年4月   日本獣医学会  

    長島 寛

     詳細を見る

  • 平成14年大会優秀発表賞

    2002年3月   日本水環境学会  

    長島 寛

     詳細を見る

▶ 全件表示

共同研究・競争的資金等の研究

  • カメの甲羅の発生的由来から考える骨格形成の進化

    研究課題/領域番号:24K09995

    2024年4月 - 2027年3月

    制度名:科学研究費助成事業

    研究種目:基盤研究(C)

    提供機関:日本学術振興会

    長島 寛

      詳細を見る

    配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

    researchmap

  • 哺乳類特異的構造、横隔膜を進化させた発生機構の解明

    研究課題/領域番号:21K06751

    2021年4月 - 2024年3月

    制度名:科学研究費助成事業

    研究種目:基盤研究(C)

    提供機関:日本学術振興会

    長島 寛

      詳細を見る

    配分額:4290000円 ( 直接経費:3300000円 、 間接経費:990000円 )

    ヒトの呼吸は胸式呼吸と腹式呼吸がある。肺自体には筋がないから、肺を容れるカゴである胸郭の容積を変えて、間接的に肺を膨らませたり縮ませたりしている。呼気は主に膨らんだ胸郭や肺が元に戻る復元力である弾性収縮力によって行われるが、吸気は胸郭の容積を拡大して行われる。胸式呼吸では肋骨を引き上げて胸郭を広げ、腹式呼吸では胸郭の底に張っている横隔膜を緊張させて底を下げ胸郭を拡大している。安静時には主に腹式呼吸が働いている。この横隔膜は哺乳類で進化した形質であるが、それがどのような発生基盤を元にして進化したのか不明である。横隔膜は頸部で作られるが、まずコントロールとして、横隔膜のないニワトリの頸神経の発生の特徴を明らかにしようと試みた。ニワトリの前肢、後肢の間に肢芽を移植すると、形成された過剰肢には筋と胸神経が侵入するが、頸部に肢芽を移植しても筋は侵入するものの、頸神経は侵入しないことが知られている。この要因を探るため、肢芽と共に様々な組織を頸部に移植したところ、肢芽に隣接する体節を移植した時に過剰肢に頸神経が侵入することが分かった。さらに頸神経の伸長に肢芽領域体節からの栄養因子が必要なのか、あるいは頸神経の伸長を頸部体節が阻害しているのかを確かめるため、頸部に肢芽を移植し同時に頸部体節を除去したところ、過剰肢に頸神経が侵入した。よって頸部体節が過剰肢への軸索侵入を阻害していたのである。しかしながら、前肢部の体節を頸部体節へ入れ替えても前肢に神経線維が入ったので、頸部体節による軸索伸長阻害は頸神経に対してだけ有効なようである。これは正常発生においては頸神経が前肢に誤って侵入するのを頸部体節が防いでいる、換言すれば腕神経叢の頭側端の位置を頸部体節が決定している可能性を示唆していると考えられた。

    researchmap

  • 比較・実験発生学からさぐる頸部の筋、神経系の進化

    2018年4月 - 2020年3月

    制度名:科学研究費補助金, 基盤研究(C)

    提供機関:日本学術振興会

    長島 寛

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    researchmap

  • 頸部およびそれに関わる解剖学的諸構造の比較、実験発生学的解析

    2015年4月 - 2018年3月

    制度名:科学研究費補助金, 基盤研究(C)

    提供機関:日本学術振興会

    長島 寛

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    researchmap

  • 脊椎動物頸部の比較解剖学的、比較発生学的解析

    2012年4月 - 2015年3月

    制度名:科学研究費補助金, 基盤研究(C)

    提供機関:日本学術振興会

    長島 寛

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    researchmap

  • 四肢に対する新たな考え方の提案

    2012年 - 2015年

    制度名:医学系研究奨励(基礎)

    提供機関:武田科学振興財団

    長島 寛

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    researchmap

  • 頸は頭か胴体か? -発生、進化から頸部の解剖学的理解を求めて-

    2010年4月 - 2012年3月

    制度名:研究費補助金, 若手研究(B)

    提供機関:日本学術振興会

    長島 寛

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    researchmap

  • カメの比較発生解剖学から分かること -肩帯、胸部形態を中心に-

    2008年4月 - 2010年3月

    制度名:科学研究費補助金, 若手研究(B)

    提供機関:日本学術振興会

    長島 寛

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    researchmap

▶ 全件表示

 

担当経験のある授業科目

  • 人体の構造と機能I(肉眼解剖学)

    2024年
    -
    現在
    機関名:新潟大学

  • 人体の構造と機能II(神経の構造)

    2024年
    -
    現在
    機関名:新潟大学

  • 人体の構造と機能II(組織学各論)

    2024年
    -
    現在
    機関名:新潟大学

  • 人体の構造と機能I(組織学総論)

    2024年
    -
    現在
    機関名:新潟大学

  • 人体の構造と機能I(解剖総論)

    2024年
    -
    現在
    機関名:新潟大学