Updated on 2024/04/19

写真a

 
SHIGA Takashi
 
Organization
Academic Assembly Institute of Humanities and Social Sciences KYOIKUGAKU KEIRETU Associate Professor
Faculty of Education Mathematical and Natural Sciences Associate Professor
Graduate School of Science and Technology Environmental Science and Technology Natural Environmental Science Associate Professor
Title
Associate Professor
External link

Degree

  • Doctor (Science) ( 2007.4   Kobe University )

Research Interests

  • 生物地理学

  • 系統分類学

  • 雑種形成

  • 水生植物

Research Areas

  • Life Science / Biodiversity and systematics

  • Humanities & Social Sciences / Museology

  • Humanities & Social Sciences / Cultural assets study

  • Environmental Science/Agriculture Science / Conservation of biological resources

Research History (researchmap)

  • 新潟大学 自然科学研究科 環境科学専攻   准教授

    2018.4

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  • Niigata University Faculty of Education Chair of Mathematical and Natural Sciences   Associate Professor

    2012.4

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  • Osaka Museum of Natural History   Curator

    2006.4 - 2012.3

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Research History

  • Niigata University   Graduate School of Science and Technology Environmental Science and Technology Natural Environmental Science   Associate Professor

    2018.4

  • Niigata University   Faculty of Education Mathematical and Natural Sciences   Associate Professor

    2012.4

  • Niigata University   Graduate School of Education School Subjects   Associate Professor

    2012.4 - 2018.3

Professional Memberships

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Committee Memberships

  • 日本植物分類学会   評議員  

    2017.1 - 2020.12   

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  • 荒川たんぽの保全・創出検討会   検討委員  

    2016.3 - 2017.3   

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    Committee type:Municipal

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  • 日本植物分類学会   植物分類学の将来の発展と普及に関する委員会 委員  

    2015.12   

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    Committee type:Academic society

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  • 新潟市環境審議会   委員  

    2014.8   

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    Committee type:Municipal

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  • 佐潟周辺自然環境保全連絡協議会   会員  

    2014.8 - 2019.3   

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    Committee type:Municipal

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  • 新潟市里潟学術研究審査会   審査委員  

    2014.6 - 2018.3   

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    Committee type:Municipal

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  • 新潟市立潟環境研究所   客員研究員  

    2014.4 - 2019.3   

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    Committee type:Municipal

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  • ビュー福島潟(新潟市)指定管理者申請者評価会議   委員  

    2013.7 - 2013.10   

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    Committee type:Municipal

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  • 新潟市佐潟等学術研究審査会   審査委員  

    2013.6 - 2014.5   

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    Committee type:Municipal

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  • 日本植物分類学会   絶滅危惧植物第一専門委員会 委員  

    2013.1   

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    Committee type:Academic society

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  • 日本植物分類学会   庶務幹事  

    2013.1 - 2016.12   

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    Committee type:Academic society

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  • 東北植物研究会   編集委員  

    2012.11   

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    Committee type:Academic society

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  • 県下生物・岩石標本展(長岡市)   審査委員  

    2012.10 - 2020.3   

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    Committee type:Municipal

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  • 新名神高速道路大阪府域自然環境保全検討委員会   委員  

    2011.9 - 2018.3   

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    Committee type:Other

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  • 岐阜市   自然環境アドバイザー  

    2010.9   

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    Committee type:Municipal

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  • 大阪府   絶滅危惧植物主任調査員  

    2010.9 - 2012.3   

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    Committee type:Municipal

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  • 近畿植物同好会   顧問  

    2010.4   

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    Committee type:Academic society

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  • 水草研究会   幹事  

    2009.8   

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    Committee type:Academic society

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  • 環境省   希少野生動植物種保存推進員  

    2009.7   

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    Committee type:Government

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Papers

  • Two lineages of Lemna aequinoctialis (Araceae, Lemnoideae) based on physiology, morphology, and phylogeny Reviewed

    Yuri Lee, Syou Kato, Jae Young Kim, Yoshiko Shimono, Takashi Shiga

    Journal of Plant Research   2024.2

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10265-023-01509-w

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    Other Link: https://link.springer.com/article/10.1007/s10265-023-01509-w/fulltext.html

  • Database of aquatic macrophytes in Japanese lakes Reviewed

    Syou Kato, Takashi Shiga, Takashi Yamanouchi, Jun Nishihiro

    Ecological Research   1 - 12   2024.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1440-1703.12449

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  • Distribution of Nymphaea pygmaea and N. tetragona (Nymphaeaceae) in Hokkaido, Sakhalin Island, and Kuril Islands based on herbarium specimen records Reviewed

    Hanaka Naito, Takashi Shiga

    Journal of Asia-Pacific Biodiversity   16   2023.11

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.japb.2023.10.010

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  • A new distribution record of Lemna turionifera (Araceae, Lemnoideae) in Korea Reviewed

    Yuri Lee, Jae Young Kim, Gyu Young Chung, Takashi Shiga

    Journal of Asia-Pacific Biodiversity   16 ( 2 )   261 - 266   2023.6

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.japb.2023.01.001

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  • The discovery of a new locality for Aldrovanda vesiculosa (Droseraceae), a critically endangered free-floating plant in Japan Reviewed

    Shougo Nishihara, Takashi Shiga, Jun Nishihiro

    Journal of Asia-Pacific Biodiversity   16 ( 2 )   227 - 233   2023.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.japb.2023.03.013

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  • Phedimus daeamensis (Crassulaceae), a new species from Mt. Daeam in Korea Reviewed

    Tae-Young Choi, Dong Chan Son, Takashi Shiga, Soo-Rang Lee

    PhytoKeys   212   57 - 71   2022.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Pensoft Publishers  

    Phedimus individuals from Mt. Daeam, once referred to as Phedimus sikokianus, exhibit certain morphological characters that are unique within the genus. Phedimus is one of the most notorious groups for taxonomic problems due to the high morphological variation found in leaf shape, stem numbers, phyllotaxis and seed structure. Taxa in Phedimus also easily hybridize, further leading to taxonomic confusion. To carefully confirm the identity of the putative new species from Mt. Daeam, we examined morphological characters from ~100 herbarium sheets of six closely related Phedimus species. A molecular phylogenetic approach was also employed to delimit the species boundary and infer the phylogenetic relationships among the seven Phedimus species, including the species from Mt. Daeam. Both morphological and molecular phylogenetic results indicated that the species found on Mt. Daeam is a new species that is more closely related to P. middendorffianus and P. takesimensis than to the remaining four Phedimus species. Here, we provided a full description of the new species P. daeamensis as well as an updated key for the seven Phedimus species examined.

    File: PK-212-057_article-82604_en_1.pdf

    DOI: 10.3897/phytokeys.212.82604

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    Other Link: https://phytokeys.pensoft.net/article/82604/download/xml/

  • Obtaining new records of critically endangered Potamogeton praelongus (Potamogetonaceae) depending on groundwater springs in northern coastal areas of Hokkaido, Japan Reviewed

    Kohtaroh Shutoh, Tomoki Hirose, Takashi Shibahara, Mami Yamazaki, Takashi Shiga

    Journal of Asia-Pacific Biodiversity   15 ( 4 )   665 - 670   2022.8

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.japb.2022.07.004

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  • Vascular plant flora of Oze, Japan and its revision Reviewed

    Ohmori Takehiro, Kurosawa Takahide, Shiga Takashi, Usuba Mitsuru, Nemoto Shuichi, Yoshii Hiroshi, Ebihara Atsushi, Tanaka Norihisa, Amano Makoto

    80   175 - 197   2022.3

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  • Comments on the updated list of vascular plants from Oze, Japan Reviewed

    Ohmori Takehiro, Kurosawa Takahide, Shiga Takashi, Usuba Mitsuru, Nemoto Shuichi, Yoshii Hiroshi, Ebihara Atsushi, Tanaka Norihisa, Amano Makoto

    80   199 - 223   2022.3

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  • Aquatic plant flora in Lake Oze-numa, and surrounding wetlands, Japan. Reviewed

    Usuba Mitsuru, Shiga Takashi, Kato Syou, Kurosawa Takahide, Nemoto Shuichi, Midorikawa Shotaro, Yamanouchi Takashi, Ohmori Takehiro

    80   225 - 235   2022.3

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    Language:Japanese  

    A combination of field and herbarium surveys recorded a total of 34 plant species, one variety of aquatic vascular plant, two aquatic moss species and two charophyte species in Lake Ozenuma and the wetlands surrounding the lake. As a result of the surveys, Acorus calamus L., Typha latifolia L., Potamogeton octandrus Poir. and Schoenoplectus nipponicus (Makino) Soják were newly recorded in the area. However, the former two species are presumed to have occurred in the region in the early of the 2000s. Further, four of the species that were previously recorded as being distributed in the region were considered to have been misidentified; i.e. specimens previously identified as P. nipponicus Makino, S. tabernaemontani (C.C.Gmel.) Palla, Myriophyllum spicatum L. and Utricularia intermedia Heyne were identified as P. gramineus L., S. lacustris (L.) Palla, M. verticillatum L. and U. ochroleuca R.W.Hartm in this study, respectively.

    DOI: 10.14943/lowtemsci.80.225

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  • Notes on the taxonomy of Nymphaeaceae and Menyanthaceae in Mongolia Reviewed

    Shukherdorj Baasanmunkh, Batlai Oyuntsetseg, Magsar Urgamal, Joscelyn Norris, Takashi Shiga, Hyeok Jae Choi

    Journal of Asia-Pacific Biodiversity   15 ( 1 )   129 - 137   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.japb.2021.09.011

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  • Sagittaria siamaginashi (Alismataceae): a new species from Thailand and Myanmar Reviewed

    Takashi Shiga, Syou Kato, Katsuyoshi Suzuki, Kazumasa Tsubota, Siriporn Zungsontiporn, Tanchanok Jongrukthai, Ansaya Promma, Akekarat Tanutong, Mu Mu Aung, Norio Tanaka, Qixiang Lu, Kazuyuki Itoh

    Acta Phytotaxonomica et Geobotanica   73 ( 1 )   35 - 47   2022.2

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.18942/apg.202022

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  • A New Record of Carex capricornis (Cyperaceae) from Mongolia Reviewed

    Okihito Yano, Takashi Shiga, Khurelbaatar Khaliunaa, Shukherdorj Baasanmunkh, Batlai Oyuntsetseg, Hyeok Jae Choi

    The Journal of Japanese Botany   96 ( 4 )   238 - 241   2021.8

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  • Taxonomic identity of Landoltia punctata (Araceae, Lemnoideae) in Korea Reviewed

    Yuri Lee, Hyeok Jae Choi, Takashi Shiga

    Journal of Asia-Pacific Biodiversity   13 ( 3 )   494 - 498   2020.9

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.japb.2020.04.003

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  • New Mongolian records of two genera, seven species, and two hybrid nothospecies from Khar-Us Lake and its associated wetlands Reviewed

    Takashi Shiga, Khurelbaatar Khaliunaa, Shukherdorj Baasanmunkh, Batlai Oyuntsetseg, Shotaro Midorikawa, Hyeok Jae Choi

    Journal of Asia-Pacific Biodiversity   13 ( 3 )   443 - 453   2020.9

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.japb.2020.06.008

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  • Evidence for newly discovered albino mutants in a pyroloid: implication for the nutritional mode in the genus Pyrola Reviewed

    Kohtaroh Shutoh, Yuko Tajima, Jun Matsubayashi, Ichiro Tayasu, Syou Kato, Takashi Shiga, Kenji Suetsugu

    American Journal of Botany   107 ( 4 )   650 - 657   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/ajb2.1462

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ajb2.1462

  • An easy method of identifying herbarium specimens of Najas minor and N. oguraensis Reviewed

    Midorikawa S, Shutoh K, T. Shiga

    Acta Phytotaxonomica et Geobotanica   71 ( 1 )   55 - 63   2020.2

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  • A new record of Potamogeton × angustifolius J.Presl (Potamogetonaceae) in Japan Reviewed

    Shutoh K, M. Usuba, H. Yamagishi, Y. Fujii, T. Shiga

    Acta Phytotaxonomica et Geobotanica   71 ( 1 )   33 - 44   2020.2

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  • Contribution to the knowledge on the flora of northern Mongolia Reviewed

    S. Baasanmunkh, B. Oyuntsetseg, O. Khurelpurev, K. Khaliunaa, U. Magsar, B. Nyam-Osor, T. Shiga, G. Y. Chung, H. J. Choi

    Journal of Asia-Pacific Biodiversity   12 ( 4 )   643 - 660   2019.12

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    DOI: 10.1016/j.japb.2019.08.009

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  • Present status of the local plant societies in Japan : A consideration based on the questionnaire Reviewed

    Kadono Y, H. Ikeda, A. Ebihara, H. Kamiaka, S. Kariyama, T. Kurosawa, D. Sakuma, T. Shiga, K. Suzuki, M. Suzuki, H. Setoguchi, M. Takamiya, A. Takano, S. Fujii, K. Fujikawa, M. Mochida

    The journal of phytogeography and taxonomy   67 ( 2 )   165 - 178   2019.11

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

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  • The aquatic macrophyte flora of a small pond revealing high species richness in the Aomori Prefecture, Japan Reviewed

    Shutoh, Kohtaroh, Yamanouchi, Takashi, Kato, Syou, Yamagishi, Hiroki, Ueno, Yusuke, Hiramatsu, Shiori, Nishihiro, Jun, Shiga, Takashi

    Journal of Asia-Pacific Biodiversity   12 ( 3 )   448 - 458   2019.9

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATL SCIENCE MUSEUM & KOREAN NATL ARBORETUM  

    A small semiartificial pond, revealing high species richness with respect to the aquatic macrophytes, was studied in the Aomori Prefecture, Japan. The pond had been completely formed ca. 10 years previously after excavation into a wet bog. To identify and evaluate its aquatic macrophyte flora during 2017-2018, we listed the species of aquatic vascular plants and charophytes in the pond and compared this list with those from the natural Japanese lakes using a database of aquatic flora in Japan. Two species were identified by molecular analyses because they lacked any reproductive organs, which were necessary for identifying the species level. We found a total of 57 taxa of aquatic macrophytes including 15 Red List species in Japan or Aomori Prefecture. Comparing the flora list with those from the 66 natural Japanese lakes surveyed since 2001, the pond was ranked fourth in the order of diversity (based on species richness) and rarity (based on numbers of Red List species). Therefore, the pond is an important aquatic environment in terms of aquatic macrophyte conservation in Japan. Interestingly, for a pond with such high species diversity, it is relatively small (0.14 km(2)), semiartificial, and relatively recently formed. (C) 2019 National Science Museum of Korea (NSMK) and Korea National Arboretum (KNA), Publishing Services by Elsevier.

    DOI: 10.1016/j.japb.2019.02.006

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  • The Identity of an Alien Utricularia (Lentibulariaceae) Naturalized in Japan Reviewed

    Kadono Y, T.Noda and, K.Tsubota, K.Shutoh, T.Shiga

    Acta Phytotaxonomica et Geobotanica   70 ( 2 )   129 - 134   2019.6

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    DOI: 10.18942/apg.201820

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  • Contribution to the knowledge on the flora of Numrug Strictly Protected Area and some parts of East Mongolia Reviewed

    Baasanmunkh S., T. Shiga, B. Oyuntsetseg, K. Wesche, C. Ritz, K. Khurelbaatar, J. Y. Kim, H. J. Jo, B. Nyam-Osor, G. Y. Chung, H. J. Choi

    Journal of Asia-Pacific Biodiversity   12 ( 2 )   284 - 301   2019.6

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    DOI: 10.1016/j.japb.2019.01.005

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  • Molecular phylogenetic relationships among populations of Sagittaria aginashi Makino (Alismataceae) and endemic Chinese species Reviewed

    Tsubota K, K. Shutoh, S. Kato, H.-J. Choi, T. Shiga

    Journal of Asia-Pacific Biodiversity   12 ( 1 )   106 - 114   2019.3

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    DOI: 10.1016/j.japb.2018.11.007

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  • Distribution of Aquatic Plants, Charalean algae, and Hygrophytes in Lake Kawaguchi, at the northern foot of Mt. Fuji in 2017 Reviewed

    Serisawa Y, S. Nakamura, S. Kato, T. Shiga, T. Yamanouchi, K. Shutoh, K. Tsubota, S. Midorikawa, T. Uejima, R. Watanabe, T. Ito, T. Nakamura, S. Yamamoto, K. Matsuyama-Serisawa

    Mount Fuji Research   13 ( 13 )   17 - 27   2019.3

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.24769/00000066

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  • 尾瀬ヶ原におけるコウホネ属植物の繁殖器官の色彩多型とウリュウコウホネNuphar pumila var. ozeensis f. rubro-ovariaの分類学的な妥当性について

    志賀 隆, 薄葉 満, 山口昌子, 齋藤真香, 黒沢高秀, 大森威宏

    水草研究会誌   ( 107 )   27 - 32   2018.11

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  • A New Record of the Critically Endangered Pondweed, Potamogeton lucens (Potamogetonaceae) from Aomori Prefecture, Japan Reviewed

    Shutoh K, M. Usuba, H. Yamagishi, Y. Fujita, S. Hiramatsu, O. Tsujimura, Y. Ishidoya, M. Kasai, N. Kasai, A. Matsumoto, T. Norita, A. Yokoyama, S. Kaneko, T. Shiga

    The Journal of Japanese botany   93 ( 4 )   240 - 252   2018.8

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    Authorship:Last author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:ツムラ  

    A new locality for Potamogeton lucens L. (Potamogetonaceae) was recorded from a pond in Aomori Pref., Tohoku District, Japan. In Japan, this species is critically endangered, and the sole remaining naturally established population was previously known only from one other locality. In June 2017, we found fragments of a submerged plant species that resembled P. lucens. We conducted fi eld surveys and identifi ed it as P. lucens based on morphological measurements, nuclear and chloroplast molecular analyses, a herbarium specimen survey, and pollen fertility. Although we could not reveal its population size and distribution in the pond, at least an established population exists because many fragments and a plant with developed roots were collected. This new locality of P. lucens represents the new northern limit of the species in Japan, which is over 500 km away from its nearest distributional record. As developments are now in progress around the pond area, urgent protection by a conservation program is needed.

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  • A new record of Stuckenia pectinata( L.) Börner( Potamogetonaceae) in Osaka Prefecture, Japan Reviewed

    Kohtaroh Shutoh, Masashi Yokogawa, Takashi Shiga

    Bulletin of the Osaka Museum of Natural History   72   47 - 51   2018.3

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Stuckenia pectinata( Potamogetonaceae) was newly recorded in Osaka Prefecture, Japan. We collected the species from the Kumeda-ike pond in Kishiwada-shi, in September 2017.

    DOI: 10.20643/00001294

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  • Current status and changes in aquatic macrophyte flora of the Kumeda-ike Pond, in Kishiwada, Osaka Prefecture, Japan

    Masashi YOKOGAWA, Kohtaroh SHUTOH, Miho KAZAMA, Takashi SHIGA

    Bull. Nat. Hist. Mus. Kishiwada   5   1 - 9   2018

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    Language:Japanese  

    We investigated aquatic flora of the Kumeda-ike pond in Kishiwada City, Osaka Prefecture in September, 2017. With the help of this study, 12 species of aquatic vascular plants and one stonewort species were recorded including three endangered species. The most dominant species were Myriophyllum spicatum L., Stuckenia pectinata (L.) Börner, and Potamogeton pusillus L.. Electric conductivity of the Kumeda-ike pond was 2.57 mS/cm. We attempted to reconstruct the past aquatic flora of the Kumeda-ike pond using records of specimens, literature, and personal observation. It was revealed that various aquatic plants were present in the 1950s according to the specimen records. These aquatic plants disappeared perhaps by eutrophication of pond in the 1980s according to the literature record, and an invasive species, Elodea nuttallii (Planch.) St. John, dominated the pond in the early 2000s according to personal observation records. Thereafter, the dominant species has been replaced by M. spicatum L. since around 2007. The aquatic flora of the Kumeda-ike pond has possibly changed with varying water quality.

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  • Distribution records of aquatic plants of Tokai region from 1967 to 2010: the catalog of specimens donated to Osaka Museum of Natural History by Mr. Shigetaka Hamashima.

    Takashi SHIGA, Shigetaka HAMASHIMA

    SHIZENSHI-KENKYU, Occasional papers from the Osaka Museum of Natural History   4 ( 1 )   1 - 16   2017.12

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    Language:Japanese   Publisher:大阪市立自然史博物館  

    Mr. Shigetaka Hamashima donated his aquatic plant specimens to Osaka Museum of Natural History (OSA) on December 2010. This collection consists of 28 families, 129 taxa, and 1028 specimens collected from 1967 to 2010. These specimens were collected at 26 prefectures, mainly in Tokai region : Aichi Prefecture (542 specimens, 52.7%), Gifu Prefecture (203 specimens, 19.7%), and Mie Prefecture (124 specimens, 12.1%). These specimens are important collections for knowledge of wetland natural history in Tokai region. In this paper, we list the aquatic specimens donated to OSA and the related literature of Mr. Hamashima.

    DOI: 10.20643/00001280

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  • Genetic Identification of Sagittaria natans (Alismataceae) from Lake Yonuma (Iwate Prefecture) as a New Locality in Japan Reviewed

    Shiga T, S. Kato, M. Usuba, T. Yamanouchi, T. Kurazono, Y. Hirasawa, M. Yamazaki

    The journal of Japanese botany   92 ( 4 )   184 - 192   2017.8

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ツムラ  

    We identified Sagittaria plants distributed in Lake Yonuma (Iwate Prefecture, Japan) via DNA sequencing and also studied the morphology, distribution, and habitat at Lake Yonuma during 2012 and 2015. In addition, we cultivated plants in an outdoor aquarium. The internal transcribed spacer (ITS) region sequence of these unidentified Sagittaria plants completely matched that of S. natans Pall. from Hokkaido, Heilongjiang, Inner Mongolia, Sakhalin, and other GenBank data. In the field, almost all plants sprouted from vegetative propagules and tubers remaining on the parent plants. Some floating-leaved plants had stolons and new tubers. Although no plants flowered, under natural or cultivated conditions, genetic and morphological characteristics indicate that the plants were S. natans. The population in Lake Yonuma is a remnant population of S. natans in Japan and is located at the southeastern extent of the species distribution worldwide. This population of rare plants requires urgent protection.

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  • Cytogeographical studies on Fallopia japonica and F. sachalinensis in Japan, by means of flow cytometry Reviewed

    Ohsawa H, T. Morita, T. Shiga, K. Okazaki

    The journal of phytogeography and taxonomy   65 ( 1 )   7 - 13   2017.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:植物地理・分類学会  

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  • Genetic diversity and population structure of Nuphar submersa (Nymphaeaceae), a critically endangered aquatic plant endemic to Japan, and implications for its conservation Reviewed

    Takashi Shiga, Masashi Yokogawa, Shingo Kaneko, Yuji Isagi

    JOURNAL OF PLANT RESEARCH   130 ( 1 )   83 - 93   2017.1

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    Nuphar submersa (Nymphaeaceae) is a critically endangered freshwater macrophyte indigenous to central Japan, with only four small extant populations represented across its entire range. We investigated the genotypic and genetic diversity as well as the genetic structure of all extant individuals of N. submersa based on analysis of 15 microsatellite loci. Among 278 individual ramets, 52 multilocus genotypes were detected: 30 genotypes in Nikko City (NIK), 18 in Nasukarasuyama City (NAS), 3 in Mooka City (MOK), and 1 in Sakura City (SAK). The average number of alleles per locus ranged from 1.20 to 1.93, whereas the observed and expected heterozygosities ranged from 0.11 to 0.33 and from 0.10 to 0.24, respectively. With the exception of SAK, all populations contained multiple clones, but our results indicated low levels of within-population genetic diversity. The populations NIK and NAS comprised few large or middle-sized genets and many small genets. The populations NIK and NAS were suggested to comprise large old, old fragmented, and/or young small genets resulting from seedling establishment. All four populations were differentiated, and gene flow between the populations was restricted (average level of gene flow (Nm) = 0.122, G' (ST) = 0.639). Of the total genetic diversity, 67.20 and 9.13% were attributable to inter- and intra-population diversity, respectively. STRUCTURE analysis revealed two or three well-differentiated groups of populations. Cluster I comprised one population (NIK) and cluster II comprised the remaining populations at K = 2. The populations NIK, NAS, and the remaining populations were assigned to clusters I, II, and III, respectively, at K = 3. For conservation practices, we recommend that each cluster be regarded as a different management unit. We further suggest that artificial gene flow among MOK and SAK populations is an appropriate option, whereas NIK should not be reinforced with genotypes from the remaining populations.

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  • Actual distribution and present status of threatend aquatic plant, Sagittaria aginashi (Alismataceae), in Korea Reviewed

    Shiga T, K. Tsubota, H. J. Choi

    Korean Journal of Plant Taxonomy   47 ( 3 )   180 - 188   2017

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    We evaluated the actual distribution, present status, and number of remnant individuals of Korean Sagittaria aginashi (Alismataceae) based on herbarium specimens and field surveys. We also reidentified Korean S. aginashi by analyzing sequences of the internal transcribed spacer (ITS) region. We found nine specimens of S. aginashi in herbaria from four localities housed at the Osaka Museum of Natural History (OSA) and at the Korea National Herbarium (KH). During our field research, we could not confirm the current statuses of two collection localities (Taereung and Ansan-si), though this was not the case for Ulsan-si. In addition, we found two new localities in Ulsan-si and Yangsan-si. These three remnant populations are distributed in wetlands in mountainous areas (elev. 480–740 m). With regard to the number of flowering and immature individuals in the three localities, there were <50 and <2,000, respectively, in total. ITS sequences demonstrated that the sequences of Korean and Japanese S. aginashi are uniform and distinct from other Sagittaria species. These results indicate that S. aginashi is a rare and threatened species in Korea. It should be listed as an endangered species on the Korean Red List and requires urgent protection by conservation programs, including the extensive surveys of other possible natural habitats.

    DOI: 10.11110/kjpt.2017.47.3.180

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  • MICROSATELLITE MARKERS FOR NUPHAR JAPONICA (NYMPHAEACEAE), AN AQUATIC PLANT IN THE AGRICULTURAL ECOSYSTEM OF JAPAN Reviewed

    Toshiaki Kondo, Sonoko Watanabe, Takashi Shiga, Yuji Isagi

    APPLICATIONS IN PLANT SCIENCES   4 ( 12 )   2016.12

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    Premise of the study: Nuphar species (Nymphaeaceae) are representative aquatic plants in irrigation ponds in Japanese agricultural ecosystems. We developed 15 polymorphic microsatellite markers for N. japonica and confirmed their utility for its close relatives N. oguraensis var. akiensis and N. xsaijoensis, which originated from natural hybridization between N. japonica and N. oguraensis.Methods and Results: Genetic variation was characterized in 15 polymorphic loci in three populations of N. japonica. The average number of alleles per locus was 3.47 (range = 2-9; n = 32), and the average expected heterozygosity per locus was 0.84 (range = 0.5-1.0); 11 loci were amplified in N. oguraensis var. akiensis and 15 in N. xsaijoensis.Conclusions: The polymorphic microsatellite markers developed in this study will be useful for investigating the levels of genetic diversity within remnant populations of Nuphar taxa and could provide a valuable tool for conservation genetics of these taxa.

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  • Newly naturalized plant Vallisneria × pseudorosulata, and identification of V. australis

    FUJII Shinji, MAKI Masayuki, SHIGA Takashi

    Bulletin of Water Plant Society   ( 103 )   8 - 12   2016.3

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  • Recovery of genetic diversity in threatened plants through use of germinated seeds from herbarium specimens Reviewed

    Naoyuki Nakahama, Yuki Hirasawa, Tsubasa Minato, Masahiro Hasegawa, Yuji Isagi, Takashi Shiga

    PLANT ECOLOGY   216 ( 12 )   1635 - 1647   2015.12

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    The reintroduction of ex situ conserved individuals is an important approach for conserving threatened plants and reducing extinction risk. In this study, we elucidated the effects on the genetic diversity of wild populations of Vincetoxicum pycnostelma Kitag. [=Cynanchum paniculatum (Bunge) Kitag.] by modelling the genetic consequences of reintroducing plants using the germinated seeds of herbarium specimens. This semi-natural grassland herb is threatened in Japan. First, we tested the germinability of seeds from herbarium specimens collected from nine sites in Kinki and Tokai districts, Japan (one specimen per site, total 206 seeds). Next, we analysed the genetic diversity and structure of germinated seedlings and the current wild individuals using nine polymorphic microsatellite markers. Germination was observed for 38 seeds (18.4 %) from four specimens collected 3-18 years prior to the study. Although the genetic diversity of the specimens' seeds was lower than that of the wild population because of the small sample size, the seedlings from specimens taken from three sites had unique alleles that did not exist in the wild populations. Consequently, viable herbarium specimens' seeds with unique alleles could be useful resources for recovering the genetic diversity in threatened wild plant populations.

    DOI: 10.1007/s11258-015-0547-8

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  • Nuphar saikokuensis (Nymphaeaceae), a new species from central to western Japan Reviewed

    Shiga T, Y. Kadono

    The Journal of Japanese Botany   90 ( 1 )   20 - 26   2015.2

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  • First record of Myriophyllum oguraense Miki (Haloragaceae) in Korea Reviewed

    Choi H. J, J. E. Jang, S. W. Cheong, T. Shiga

    Korean Journal of Plant Taxonomy   44 ( 2 )   77 - 80   2014.2

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    Here we report a previously unrecorded species of Korean Myriophyllum L. (Haloragaceae). This taxon, M. oguraense Miki has been regarded as a Japanese endemic for some 60 years. Myriophyllum oguraense and its closely related M. verticillatum L. share the characteristic of having pectinate emergent leaves that are similar in shape to, but much smaller than, the submerged leaves. However, the primary characteristic that differentiates these taxa is the turion shape (club shaped in M. verticillatum and linear in M. oguraense). The common name, ‘Gin-dong-a-mul-su-sae-mi’ was also newly given considering its characteristic turion shape. Photographs and a key to Korean Myriophyllum species are provided in addition to complete descriptions including information on nomenclatural types, distributions and specimens examined.

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  • Yearly changes of vegetation areas and flowering numbers of Nuphar submersa (Nymphaeaceae)

    SHIGA TAKASHI, IKARASHI ASUKA, ABE TOMOMI, HIRASAWA YUKI, ZUSHI MASATO, SHIBATA YOSHIKO

    Bulletin of Water Plant Society   ( 100 )   51 - 60   2013.11

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  • Genetic identification of traded plants of the endangered macrophytes Nuphar submersa and N. ×fluminalis (Nymphaeaceae) based on genotype data of all remnant individuals growing in the wild Reviewed

    Takashi Shiga, Masashi Yokogawa, Shingo Kaneko, Yuji Isagi

    Japanese Journal of Conservation Ecology   18 ( 1 )   33 - 44   2013.5

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    Nuphar submersa and N. ×fluminalis (Nymphaeaceae) are critically endangered macrophytes found in central Japan, comprising only four populations each. In recent years, gathering for horticultural trade has damaged the remnant populations. We investigated the species and localities involved in trading plants of these species and Nuphar plants named "Nagaba-benikohone" based on morphological research and analysis of 15 microsatellite loci. We bought 10 plants from two garden stores. Our morphological research identified a plant named "N. submersa" sold by Store T (T1) as N. submersa and nine plants sold by Store C (C1-C9) as N. ×fluminalis. In these 10 plants, two multi-locus genotypes were detected. Referring to the genotype data for N. submersa and N. ×fluminalis, the genotype of T1 corresponded to NIK-25 (N. submersa), and the other nine plants (C1-C9) corresponded to SAN-10 (N. ×fluminalis). The low genotype probability (GP) indicated that the traded plants and wild plants were the same genets and gathering had occurred in populations NIK and SAN. Since our preliminary genetic identification of trading in Nuphar was published, there have been no trades on the web sites. Genotype data based on all remnant individuals will be useful for assisting in preventing the gathering and trade of these plants.

    DOI: 10.18960/hozen.18.1_33

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  • A relationships among curator, collector, and user surrounding natural history specimens : toward a better specimen preservation, collection and usage(Museum and ecology (22)) Reviewed

    Shiga T

    JAPANESE JOURNAL OF ECOLOGY   63 ( 3 )   375 - 383   2013

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    DOI: 10.18960/seitai.63.3_375

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  • Development of nuclear microsatellite markers for the critically endangered freshwater macrophyte, Nuphar submersa (Nymphaeaceae), and cross-species amplification in six additional Nuphar taxa Reviewed

    Masashi Yokogawa, Takashi Shiga, Shingo Kaneko, Yuji Isagi

    Conservation Genetics Resources   4 ( 2 )   295 - 298   2012

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    We developed and characterized 13 microsatellite markers for Nuphar submersa, a critically endangered freshwater macrophyte of central Japan. In all the studied populations, ten out of isolated 13 loci showed polymorphism. The 13 loci showed one to five alleles per locus, with observed and expected heterozygosities ranging from 0 to 0.40 and from 0 to 0.67, respectively. Between 5 and 11 of the 13 loci were successfully amplified across the six other Nuphar taxa. The markers described here will be useful for a conservation genetic study of the species and informing the development of effective conservation programs of N. submersa and related species. © 2011 Springer Science+Business Media B.V.

    DOI: 10.1007/s12686-011-9528-y

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  • 全個体遺伝子型解析による絶滅危惧植物の保全 Reviewed

    井鷺裕司, 兼子伸吾, 水谷未耶, 加藤慶子, 伊津野彩子, 高宮正之, 志賀 隆, 増本育子, 大竹邦明

    DNA多型   20   148 - 152   2012

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  • The biological invasion of Sagittaria platyphylla (Enggelm.) J. G. Sm. in Yodogawa river system in Osaka Prefecture and a correct scientific name of exotic Sagittaria named "Nagaba-omodaka"

    Bulletin of Water Plant Society, Japan   ( 93 )   13 - 22   2010.5

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  • Geographic distributions of Veronica undulata and alien species, V. anagallis-aquatica, and their hybrids in Yamatogawa River system, central Japan (Japanese) Reviewed

    Takashi SHIGA, Aquatic Plant, Research Team of, Yamatogawa River, Research Group, Project Y, Osaka Museum of, Natural History

    Bulletin of the Osaka Museum of Natural History   62   65 - 74   2008.3

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  • Genetic relationships of Nuphar in central to western Japan as revealed by allozyme analysis Reviewed

    Takashi Shiga, Yasuro Kadono

    AQUATIC BOTANY   88 ( 2 )   105 - 112   2008.2

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    The genetic relationships of Nuphar japonica, N. oguraensis, N. subintegerrima and two morphologically intermediate plant groups (J-O and J-S) found in central to western Japan were investigated. Allozyme analyses (analysis of allele frequencies and principal coordinate analysis, based on shared allele distance among the 162 multi-locus genotypes) showed the three Nuphar species to be distinguished, and indicated that the intermediate plant groups were genetically intermediate either between two or among three of these species. Natural hybridization and introgression were found to have occurred among these three species in central to western Japan, producing genetically varied intermediate plant groups. Many of the intermediate plants (74.4% of the total numbers of genotypes of the intermediate plants) did not show additive combinations of parental allozymes, which were expected in F-1 hybrids. The absence of characteristic parental bands suggested that sexual reproduction had continued among the hybrids beyond the F-1. (c) 2007 Published by Elsevier B.V.

    DOI: 10.1016/j.aquabot.2007.08.014

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  • Lectotypification of Myriophyllum oguraense (Haloragaceae) Reviewed

    Dong Wang, Zhen-yu Li, Takashi Shiga

    NOVON   18 ( 3 )   395 - 396   2008

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    The lectotype is designated for Myriophyllum oguraense Miki, a species described from japan in 1934. This taxon is listed as all endangered Species in Japan. Among Asian taxa, M. oguraense is distinguished by its monoecious habit and glauucous leaves.

    DOI: 10.3417/2006152

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  • 絶滅危惧種シモツケコウホネ(スイレン科)の生活史-根茎成長と開花フェノロジーに注目して-

    志賀 隆

    環境科学総合研究所年報   27   1 - 11   2008

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  • Natural hybridization of the two Nuphar species in northern Japan: Homoploid hybrid speciation in progress? Reviewed

    Takashi Shiga, Yasuro Kadono

    AQUATIC BOTANY   86 ( 2 )   123 - 131   2007.2

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    We investigated morphological and allozyme variation and pollen viability in 22 populations of Nuphar japonica, N. pumila, and unidentified intermediate plants in Hokkaido, Japan, to assess hybridization between the two species and the potential existence of homoploid hybrid speciation. A phenogram based on cluster analysis using 15 morphological characters revealed three cluster groups. Groups 1 and 3 had distinctive morphological characteristics and corresponded to the published descriptions of N. japonica and N. pumila, respectively, whereas Group 2 showed intermediate values in most characteristics. In our allozyme study, many morphologically intermediate plants showed additive combinations of species-specific alleles from the two species. Pollen viability was significantly lower in percentage in intermediate populations than in the two species. Intermediate populations were found within the area of overlap between the ranges of the two species. On this evidence. we concluded that intermediate plants represent hybrids between the two species. Four hybrid populations showed different relationships between morphology and pollen viability. Hybrids in artificially disturbed populations had discontinuously intermediate morphology between their parental species and were completely fertile in some cases. It is probable that ecological selection has contributed to the discontinuous morphological patterns. As a result, homoploid hybrid speciation may be occurring in these Nuphar hybrids. (c) 2006 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.aquabot.2006.09.008

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  • A systematic study of Nuphar(Nymphaeaceae) in Japan with special reference to the role of hybridization

    SHIGA Takashi

    Thesis of doctoral degree,Kobe University   2007.2

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  • Nuphar xfluminalis, a new hybrid from central Japan Reviewed

    Shiga T, Y. Kadono

    Acta Phytotaxonomica et Geobotanica   58 ( 1 )   43 - 50   2007

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    The morphology, allozyme variation and pollen viability in nine populations of Nuphar japonica, N. submersa, and unidentified intermediate plants were examined. A phenogram based on cluster analysis using 10 morphological characters revealed three cluster groups. Groups 1 and 3 had distinctive morphological characteristics and corresponded to the published descriptions of TV. japonica and N. submersa, respectively, whereas Group 2 showed intermediate values in most characteristics between the two species. In the allozyme study, many morphologically intermediate plants showed additive combinations of species-specific alleles in two loci (lapl and mdh3) from the two species. Pollen viability was significantly lower in intermediate plants than in the two species. Based on the evidence, we concluded that the intermediate plants were of hybrid origin between N. japonica and N. submersa and described them as N.×fluminalis.

    DOI: 10.18942/apg.KJ00004609391

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  • Nuphar submersa (Nymphaeaceae), a new species from central Japan Reviewed

    Shiga T, J. Ishii, Y. Isagi, Y. Kadono

    Acta Phytotaxonomica et Geobotanica   57 ( 2 )   113 - 122   2006

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    A new species of Nuphar (Nymphaeaceae), N. submersa, from Tochigi Prefecture, central Japan, is described. Nuphar submersa is characterized by its submerged habit and narrowly oblong-triangular leaves that lack a sinus. It differs from both N. japonica and N. oguraensis in the color of the anthers and fruits. A phylogenetic study based on Amplified Fragment Length Polymorphism (AFLP) fragments showed that both N. submersa and N. oguraensis were sister taxa, but they were well separated phylogenetically.

    DOI: 10.18942/apg.KJ00004622856

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  • Classification of Nuphar subintegerrima (Casp.) Makino sensu lato (Nymphaeaceae) and its present status Reviewed

    Shiga Takashi, Kadono Yasuro

    Bunrui   5 ( 2 )   113 - 122   2005

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:日本植物分類学会  

    DOI: 10.18942/bunrui.KJ00004651874

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  • 外来植物オオカワヂシャの生育環境と種子生態

    志賀 隆, 鎌倉 久美, 草竹 啓之, 廣本 貴稔, 佐々木 靖弘, 野田 大介, 古薗 保英

    水草研究会誌   ( 80 )   11 - 19   2004.4

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  • Morphological variation and classification of Nuphar with special reference to the populations in central to western Japan Reviewed

    Shiga T, Y. Kadono

    Acta Phytotaxonomica et Geobotanica   55 ( 2 )   107 - 117   2004

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    Morphological variation in 52 populations of Nuphar japonica DC., N. subintegerrima (Casp.) Makino, N. oguraensis Miki and unidentified intermediate plants were investigated during a revision of Nuphar (Nymphaeaceae) in Japan. The phenogram of cluster analysis based on 15 morphological characters revealed five cluster groups (A〜E). Clusters A, C and E had some remarkable characters and corresponded well to the description of N. japonica, N. oguraensis and N. subintegerrima sensu stricto, respectively. In contrast, Clusters B and D showed intermediate values in most of their characters. It was concluded that the intermediate plants, which usually had been identified as N. subintegerrima, were not conspecific with N. subintegerrima s. s. and that there were two different intermediate groups in central to western Japan. Morphological relationships suggested that the two intermediate groups were of hybrid origin between N. japonica and N. oguraensis and between N. japonica and N. subintegerrima, respectively. Results from the present study proved that populations of N. subintegerrima s. s. are limited in number and distribution, and that the species is one of the most endangered plants in Japan.

    DOI: 10.18942/apg.KJ00004622816

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  • 新潟県頸城湖沼群の水生・湿生植物相

    志賀 隆, 石澤 進

    水草研究会会報   ( 74 )   1 - 22   2002.2

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Books

  • Illustrated Flora of Mongolia

    Batlai Oyuntsetseg, Shukherdorj Baasanmunkh, Nyamjantsan Nyambayar, Takashi Shiga, Okihito Yano, Andrei I. Pyak, Kae Sun Chang, Jaesang Chung, Cheul-Ho Lee, Gyu Young Chung, Hyeokjae Choi( Role: Joint author ,  Aquatic Plants)

    Korea National Arboretum of the Korea Forest Service  2023.4  ( ISBN:9791192743059

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  • A database of plants surveyed in Japanese ponds (2003)

    Takashi Shiga, Syou Kato, Ken-ichi Yokoi, Yasuro Kadono( Role: Edit)

    Wetlands International Japan  2021.1 

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  • 水生植物相調査のガイドライン(ver. 1.00)

    加藤将, 首藤光太郎, 志賀隆( Role: Joint author)

    新潟大学教育学部  2020.3 

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    Total pages:70  

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  • A checklist of wetland plants in Japan ver. 1.00

    Shutoh, K, Yamanouchi, T, Yamaguchi, M, Kato, S, Shiga, T( Role: Joint author)

    Faculty of Education, Niigata University,  2019.3 

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  • A checklist of Japanese plant names

    Yamanouchi, T, Shutoh, K, Osawa, T, Yonekura, K, Kato, S, Shiga, T

    Japan Node of Global Biodiversity Information Facility  2019.3 

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  • 越後平野における新たな地域学 みんなの潟学

    新潟市潟環境研( Role: Contributor ,  Ⅱ 生き物を育む潟13-17,Ⅳ 未来へ向けて 潟と人とのこれから43)

    新潟市潟環境研究所  2018.11  ( ISBN:9784991047107

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    Total pages:127   Responsible for pages:34-43,60-61,102-103,   Language:Japanese Book type:Scholarly book

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  • ミニガイド No31 関西の水草

    志賀 隆, 首藤光太郎, 横川昌史, 加藤 将, 稗田真也, 倉園知広, 山ノ内崇志( Role: Joint author)

    大阪市立自然史博物館(大阪)  2018.7 

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    Total pages:64   Language:Japanese Book type:Dictionary, encyclopedia

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  • 水草ハンドブック

    志賀 隆, 首藤光太郎, 横川昌史, 加藤 将, 稗田真也, 倉園知広, 山ノ内崇志( Role: Joint author)

    新潟大学教育学部  2018.7 

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    Total pages:64   Language:Japanese Book type:Dictionary, encyclopedia

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  • 改訂新版 日本の野生植物2

    大橋広好, 門田裕一, 邑田仁, 米倉浩司( Role: Contributor ,  アリノトウグサ科)

    平凡社(東京)  2016.3  ( ISBN:9784582535327

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    Total pages:640   Responsible for pages:231-232   Language:Japanese Book type:Dictionary, encyclopedia

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  • 改訂新版 日本の野生植物1

    大橋広好, 門田裕一, 邑田仁, 米倉浩司( Role: Contributor ,  スイレン科)

    平凡社(東京)  2015.12  ( ISBN:9784582535310

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    Total pages:666   Responsible for pages:46-48   Language:Japanese Book type:Dictionary, encyclopedia

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  • 第46回特別展「たまごとたね」解説書『動かないタマゴと動くタネのひみつ』

    大阪市立自然史博( Role: Contributor)

    大阪市立自然史博物館  2015.7 

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    Total pages:92   Language:Japanese Book type:Scholarly book

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  • 絶滅危惧植物図鑑レッドデータプランツ増補改訂版

    矢原徹一, 藤井伸二, 伊藤元己, 海老原淳, 永田芳男( Role: Contributor)

    山と渓谷社(東京)  2015.3  ( ISBN:9784635090452

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    Total pages:784   Language:Japanese Book type:Dictionary, encyclopedia

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  • 第45回特別展「ネコと見つける都市の自然ー家の中から公園さんぽー」解説書『都市の自然2014』

    大阪市立自然史博( Role: Contributor)

    大阪市立自然史博物館(大阪)  2014 

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  • 新しい植物分類学Ⅱ

    戸部 博, 田村 実( Role: Contributor ,  コウホネ属(スイレン科)水生植物の分類学的再検討と種間交雑)

    講談社サイエンティフィック(東京)  2012.8  ( ISBN:9784061534490

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    Total pages:335   Responsible for pages:142-157   Language:Japanese Book type:Scholarly book

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  • 第5展示室 生き物のくらし(大阪市立自然史博物館展示解説第14集)

    大阪市立自然史博( Role: Contributor)

    大阪市立自然史博物館  2011 

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  • 第42回特別展解説書「みんなでつくる淀川大図鑑ー山と海をつなぐ生物多様性」

    大阪市立自然史博( Role: Contributor)

    大阪市立自然史博物館(大阪)  2010.7 

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    Total pages:112   Language:Japanese Book type:Scholarly book

    http://omnh-shop.ocnk.net/product/1283

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  • 三木茂博士寄贈水草さく葉標本目録【附三木茂水草コレクション目録・タイプ標本画像CD】(大阪市立自然史博物館収蔵資料目録第41集)

    志賀 隆, 藤井伸二, 瀬戸 剛( Role: Contributor)

    大阪市立自然史博物館  2009 

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    Responsible for pages:42   Language:Japanese Book type:Dictionary, encyclopedia

    http://omnh-shop.ocnk.net/product/1265

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  • 干潟を考える 干潟で遊ぶ(大阪市立自然史博物館叢書3)

    大阪市立自然史博( Role: Contributor)

    東海大学出版会(秦野)  2008.5  ( ISBN:9784486017813

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    Total pages:182   Language:Japanese Book type:Scholarly book

    http://www.press.tokai.ac.jp/bookdetail.jsp?isbn_code=ISBN978-4-486-01781-3

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  • 標本の作り方(大阪市立自然史博物館叢書2)

    大阪市立自然史博( Role: Contributor)

    東海大学出版会(秦野)  2007.7  ( ISBN:9784486017691

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    Total pages:208   Language:Japanese Book type:Scholarly book

    http://www.press.tokai.ac.jp/bookdetail.jsp?isbn_code=ISBN978-4-486-01769-1

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  • 大和川の自然(大阪市立自然史博物館叢書1)

    大阪市立自然史博( Role: Contributor)

    東海大学出版会(秦野)  2007.6  ( ISBN:9784486017677

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    Total pages:156   Language:Japanese Book type:Scholarly book

    http://www.press.tokai.ac.jp/bookdetail.jsp?isbn_code=ISBN978-4-486-01767-7

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  • 第35回特別展ガイドブック「大和川の自然ーきたない川?にもこんなんいるで」

    大阪市立自然史博( Role: Contributor)

    大阪市立自然史博物館  2006 

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    http://omnh-shop.ocnk.net/product/951

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  • 内湖からのメッセージ

    西野麻知子, 浜端悦治( Role: Contributor ,  琵琶湖周辺に生育する希少植物の遺伝的性質)

    サンライズ出版(彦根)  2005.12  ( ISBN:9784883252695

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    Total pages:256   Responsible for pages:92-101   Language:Japanese Book type:Scholarly book

    http://www.sunrise-pub.co.jp/isbn978-4-88325-269-5/

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▶ display all

MISC

  • Germination potential of herbarium specimen spores of three green-spored fern species: Equisetum arvense, Osmunda japonica and Matteuccia struthiopteris

    Kodai Ishiguro, Takuro Oba, Takashi Shiga

    Bulletin of the Faculty of Education, Natural Sciences   16 ( 2 )   2024.3

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  • Contents of the exhibition "Messages from botanical specimens: History and collections of the Niigata University Herbarium" and future issues for herbarium exhibitions

    Takashi Shiga, Hanaka Naito, Syou Kato

    Bulletin of the Faculty of Education, Natural Sciences   16 ( 1 )   1 - 15   2023.10

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    File: Bulletin_16(1)_shiga_2023_l.pdf

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  • Aquatic plant specimens collected in Kurotori, Nishi-ku, Niigata City, Niigata Prefecture, Japan, during the 1900s–1920s

    Takashi Shiga

    Bulletin of Water Plant Society, Japan   ( 114 )   11 - 19   2023.1

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  • Habitat status of Podostemaceae species in 2022 in five rivers in Kagoshima Prefecture, Japan

    Takashi Yamanouchi, Hanaka Naito, Takashi Shiga, Eriko Mashima, Syou Kato

    Bulletin of Water Plant Society, Japan   ( 114 )   21 - 27   2023.1

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  • Introduction to the herbarium of Niigata University (NGU)

    Takashi Shiga

    Bulletin of Water Plant Society, Japan   ( 113 )   63 - 64   2022.8

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  • A method for preparing submerged aquatic plant specimens and its potential for use in the science education

    Syou Kato, Hanaka Naito, Tomohiro Suzui, Takashi Shiga

    14 ( 2 )   123 - 133   2022.2

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    File: 14(2)_123-133.pdf

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  • Introduction to the herbarium of Niigata University(NGU) : History, specimen collections, and their value for science education

    Takashi Shiga, Syou Kato

    Bulletin of the Faculty of Education, Natural Sciences   14 ( 2 )   111 - 122   2022.2

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    File: 14(2)_111-122.pdf

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  • A new locality of Blyxa alternifolia (Miq.) Hartog in Gifu Prefecture

    Yuri Lee, Hanaka Naito, Takashi Shiga, Syou Kato

    Bulletin of Water Plant Society, Japan   ( 111 )   37 - 39   2021.7

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  • Forty years of Water Plant Society, Japan in terms of statistical data

    Takashi Yamanouchi, Takashi Shiga

    Bulletin of Water Plant Society, Japan   ( 111 )   97 - 104   2021.7

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  • The featured articles of the 40th anniversary of Water Plant Society, Japan

    Takashi Shiga

    Bulletin of Water Plant Society, Japan   ( 111 )   53 - 54   2021.7

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  • Disappearance of Nymphaea tetragona Georgi var. erythrostigmatica Koji Ito in its type locality, Lake Kimoma-numa, Japan

    Hanaka Naito, Takashi Shiga

    Bulletin of Water Plant Society, Japan   ( 111 )   41 - 44   2021.7

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  • An introduction and supplemental information of the floristic research of aquatic macrophytes in an unnamed pond in Tsugaru, Aomori Prefecture, Japan

    Kohtaroh Shutoh, Hiroki Yamagishi, Takashi Shiga

    Bulletin of Water Plant Society, Japan   ( 110 )   21 - 31   2020.7

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  • Aquatic macrophyte flora of Numrug Strictly Protected Area and some parts of eastern Mongolia

    Takashi Shiga, Baasanmunkh Shukherdorj, Oyuntsetseg Batlai, Khaliunaa Khurelbaatar, Syou Kato, Hyeok Jae Choi

    Bulletin of Water Plant Society, Japan   ( 109 )   31 - 45   2020.2

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  • 新潟市西蒲区鎧潟干拓地における散布体バンクの分布と堆積年代の推定

    丸山紗知, 志賀隆

    平成29年度新潟市潟環境研究所研究成果報告書   15 - 25   2019.3

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  • ホザキノフサモ

    志賀 隆

    Nature Study   64 ( 9 )   1,12   2018.9

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  • 新編集担当より:水草研究会誌の新たな挑戦

    志賀 隆

    水草研究会誌   ( 107 )   1 - 2   2018

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  • 岸和田市の久米田池でオオバナミズキンバイ(広義)を確認

    横川昌史, 首藤光太郎, 志賀 隆

    Nature Study   64 ( 4 )   6,16   2018

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  • 植物標本のタネは生きている!?

    志賀 隆

    自然保護   ( 560 )   11   2017.11

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  • 博物館標本の種子は生きている! : 発芽および利用可能性と標本作製・管理方法の検討 (特集 博物館の標本情報を活用するミュゼオミクス : 過去の生物多様性を観る・測る)

    志賀 隆, 平澤 優輝, 中濱 直之, 長谷川 匡弘

    遺伝 : 生物の科学   71 ( 5 )   460 - 465   2017.9

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  • 新潟市域湖沼における水生・湿生植物相

    金田風花, 志賀 隆

    平成28年度新潟市潟環境研究所研究成果報告書   31 - 57   2017.6

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    潟環境研究所,新潟

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  • オグラノフサモとフサモの推定雑種ハリマノフサモの識別方法

    志賀 隆

    石澤進(編):新津植物資料室年報2016   2017

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    積雪地域植物研究所(新津植物資料室),新潟

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  • 幼稚園や小学校における身近な動植物を利用した活動

    志賀 隆, 工藤起来

    教育学部幼児教育研究集録   28 - 34   2017

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    新潟大学教育学部,新潟

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  • 新潟市西蒲区鎧潟干拓地の水生植物相

    丸山紗知, 志賀 隆

    平成27年度新潟市潟環境研究所研究成果報告書   36 - 44   2016.6

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    潟環境研究所,新潟

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  • Introduction to the Symposium : Future herbarium in Japan

    KUROSAWA Takahide, SHIGA Takashi

    Bunrui   16 ( 1 )   15 - 28   2016.2

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    DOI: 10.18942/bunrui.KJ00010238508

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  • Evaluation of germinability of herbarium specimens' seeds and effect on seed viability due to specimen preparation and management methods

    HIRASAWA Yuki, MINATO Tsubasa, HASEGAWA Masahiro, SHIGA Takashi

    Bunrui   16 ( 1 )   39 - 46   2016.2

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    DOI: 10.18942/bunrui.KJ00010238510

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  • 植物さく葉標本室を作ろう

    黒沢高秀, 志賀隆

    分類   16   17 - 30   2016.2

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  • 掘削地の植物相調査と土壌撒きだし試験による福島潟の埋土種子集団の解明

    志賀 隆, 渡辺雄大

    平成26年度新潟市潟環境研究所研究成果報告書   35 - 56   2015.6

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    潟環境研究所,新潟

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  • 未に似ている?-日本の水辺を彩るヒツジグサ

    志賀 隆

    Nature Study   61 ( 1 )   1 - 2,12   2015.1

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  • 松浜の池(新潟市)で確認された外来水草:オオサンショウモ、アマゾントチカガミ、ウチワゼニグサ

    志賀 隆

    新津植物資料室年報2014   231 - 232   2015

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    積雪地域植物研究所(新津植物資料室),新潟

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  • Project-Y : let's research nature of Yodogawa river system in Osaka Prefecture

    SHIGA Takashi, Plant Research Team of, Yodogawa River, Research Group(Project, Y Osaka Museum of, Natural History

    Bunrui   13 ( 1 )   21 - 29   2013

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    DOI: 10.18942/bunrui.KJ00008580346

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  • 全個体遺伝子型決定で絶滅危惧種を守る:シモツケコウホネを例にして

    志賀 隆, 横川昌史, 兼子伸吾, 井鷺裕司

    Nature Study   58 ( 3 )   2 - 4,12   2012.3

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  • 北摂でシロミノヤブムラサキの分布を確認

    志賀 隆, 菅 久

    Nature Study   58 ( 2 )   8,12   2012.2

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  • プロジェクトU都市の自然調査レポート・イヌノフグリの分布情報大募集!

    志賀 隆

    Nature Study   58 ( 2 )   16   2012.2

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  • 博物館の研究機械の紹介:DNAシーケンサー

    志賀 隆

    Nature Study   58 ( 1 )   7   2012.1

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  • 70年ぶりに大阪府でヤマサギソウを確認

    志賀 隆, 長谷川匡弘

    Nature Study   57 ( 11 )   2 - 4,12   2011.11

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  • オガルカヤ

    志賀 隆

    Nature Study   57 ( 11 )   1,12   2011.11

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    表紙 -オガルカヤ-<br />
    秋の草原はイネ科の植物で彩られます。今月の表紙の植物、オガルカヤは明るい草地に生える多年草です。花序は二つが対になって葉腋につきます。この姿がスズメが飛んでいる姿に似ていることからスズメカルカヤの別名もあります。ちなみにハーブとして使用されるレモングラスもオガルカヤ属です。吹田市にて2011年10月21日撮影。

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  • 植物の新しい分類体系:APG分類体系ってなに?

    志賀 隆

    Nature Study   57 ( 9 )   2 - 3   2011.9

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  • ハマヒルガオ

    志賀 隆

    Nature Study   57 ( 6 )   1,16   2011.6

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    表紙 - ハマヒルガオ -<br />
    海岸の砂地に生えるヒルガオ科の多年草。初夏に咲き、砂浜一面を薄ピンクに染めあげます。海からの塩分に対応するために葉は厚くなっています。2011年5月9日、大阪府阪南市貝掛海岸にて撮影。

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  • トンボに魅せられた昆虫少年が、植物の学芸員に

    志賀 隆

    県下生物標本展示会第60回記念文集 羽ばたきー私たちの県下生物標本展示会ー   60 - 66   2011

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  • The memories of the 32nd annual meeting of Water Plant Society of Japan in Osaka

    SHIGA Takashi

    Bulletin of Water Plant Society, Japan   ( 94 )   48 - 51   2010.11

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  • オグラコウホネ

    志賀 隆

    Nature Study   56 ( 8 )   1,16   2010.8

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    表紙 オクラコウホネ<br />
    河川や湖沼、ため池に生える浮葉植物。自然史博物館友の会の前身、自然科学研究会の会長をされた、三木茂博士によって発見され名付けられました。名前の由来は、干拓で消えてしまった巨椋池(おぐらいけ)から来ています。<br />
    撮影:2004年7月、兵庫県三田市。

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  • ネイチャーサロン みんなで作った「淀川大図鑑」

    志賀 隆

    Nature Study   56 ( 8 )   6   2010.8

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  • 水生植物コウホネ属(スイレン科)における「種」のあり方について

    志賀 隆

    近畿植物同好会会報   ( 109 )   13 - 15   2010.8

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  • ホナガカワヂシャ(ゴマノハグサ科)の越冬記録

    志賀 隆

    Nature Study   56 ( 7 )   4   2010.7

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  • プロジェクトY 淀川水系歩いて採って考えて(その4・植物班の巻) 淀川の水草の今を調べる

    志賀 隆

    Nature Study   56 ( 2 )   10 - 11   2010.2

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  • An introduction of water plant specimens collected by Dr. Shigeru Miki

    SHIGA Takashi

    Bulletin of Water Plant Society, Japan   ( 92 )   22 - 27   2009.12

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  • ナンキンハゼ

    志賀 隆

    Nature Study   55 ( 12 )   1,12   2009.12

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    表紙 -ナンキンハゼー<br />
    ナンキンハゼの果実は、割れると中から白い蝋物質(外種皮)に包まれた種子が3つ顔を出します(詳しくはNature Study53巻11月号の塚腰学芸員の記事をご覧ください)。皆さんのお手元にこのNature Studyが届くころにはナンキンハゼの種子はまだ残っているでしょうか?<br />
    2006年11月22日、長居植物園にて撮影。

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  • 青森で自然観察を楽しんできました!~「西津軽合宿」報告~

    志賀 隆, 寺田 玲, 松浦宜弘, 松崎妙子

    Nature Study   55 ( 10 )   7,8,16   2009.10

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  • ハスにまつわるエトセトラ

    志賀 隆

    Nature Study   55 ( 6 )   83 - 84   2009.6

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  • 新植物図鑑5 シモツケコウホネ

    藤井聖子, 志賀 隆

    趣味の山野草   30 ( 5 )   1 - 5   2009.5

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  • 越冬するボタンウキクサ‐京都市木幡池での観察‐

    志賀 隆, 武林周一郎

    Nature Study   55 ( 1 )   2 - 4,12   2009.1

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  • 淀川本流沿いでアサザを発見

    志賀 隆, 武林周一郎

    Nature Study   54 ( 12 )   164 - 165   2008.12

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  • Invasion and present status of Gymnocoronis spilanthoides (Compositae) in Lake Biwa and some notes on its eradication

    FUJII SHINJI, SHIGA TAKASHI, KANEKO YUKO, KURIBAYASHI MINORU, NOMA NAOHIKO

    Bulletin of Water Plant Society, Japan   ( 89 )   9 - 21   2008.3

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  • 水草通信 淀川下流のボタンウキクサ

    志賀 隆

    植物分類学会ニュースレター   ( 29 )   22   2008

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  • 緑の侵略者、ボタンウキクサはどこからやってくるのか?~水草の分布情報を大募集!!~

    志賀 隆

    Nature Study   53 ( 11 )   150   2007.11

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  • オギとススキ

    志賀 隆

    Nature Study   53 ( 10 )   129 - 130   2007.10

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  • 植物の雑種形成:コウホネの場合

    志賀 隆

    Nature Study   53 ( 1 )   2 - 3   2007.1

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  • 日本と外国のカワヂシャ、そしてそのこども

    志賀 隆

    Nature Study   52 ( 6 )   65 - 66   2006.6

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  • 沈水性のコウホネ属新変種の発見とその消滅の危機

    志賀 隆, 石井 潤, 角野康郎

    水草研究会誌   ( 82 )   47 - 48   2005.4

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  • 阿賀野川床固工改設に伴う陸水生物相の変化

    伊藤 章, 岡 夙男, 安部信之, 帆苅 信, 田中啓太, 志賀 隆, 本間義治

    新潟県生物教育研究会誌   38   35 - 45   2003

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    Ⅰ.藻類・動物プランクトンと河畔植物

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  • 松浜の池(阿賀野川河口右岸)における底生動物と底質

    志賀 隆, 川島慎一, 間島絵里子, 宮下直子, 田中啓太

    新潟県生物教育研究会誌   36   15 - 22   2001

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  • 新潟市松浜の池(阿賀野川河口右岸)の植物相

    志賀 隆, 川島慎一, 間島絵里子, 宮下直子, 田中啓太

    じねんじょ   23   45 - 53   2001

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  • 新潟県中頚城湖沼群の絶滅が危惧される貴重な植物

    志賀 隆

    新潟県植物保護   29   1 - 2   2001

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Presentations

  • 環境省事業「モニタリング1000」により作成された水生植物相調査マニュアルの評価

    平松 栞, 首藤光太郎, 加藤 将, 志賀 隆

    日本生態学会第65回大会  2018.3  日本生態学会

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    Venue:札幌  

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  • 日本産維管束植物の湿地依存度評価に向けて

    首藤光太郎, 山ノ内崇志, 加藤 将, 志賀 隆

    日本生態学会第65回大会  2018.3  日本生態学会

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    Venue:札幌  

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  • Herbarium specimens' seeds are still alive?:availability for regional plant conservation Invited International conference

    SHIGA Takashi

    International Symposium of Baekdudaegan National Arboretum  2017.10 

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    Venue:Chunyang  

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  • 植物標本の収集・保存・利用のこれから

    志賀 隆

    日本植物分類学会2017年度学会賞受賞記念講演  2017.3  日本植物分類学会

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    Venue:京都  

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  • 博物館標本の種子は生きている!:発芽可能性と標本作製・管理方法の検討

    志賀 隆, 平澤優樹, 中浜直之, 長谷川匡弘

    日本生態学会第64回大会  2017.3  日本生態学会

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    Venue:東京  

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  • Herbarium specimens' seeds still alive:germinability tests of herbarium seeds International conference

    Hirasawa Y, M.Hasegawa, T.Shiga

    East Asian Plant Diversity and Conservation 2016  2016.8  East Asian Plant Diversity and Conservation

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    Venue:Tokyo  

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  • Conservation and management of critically endangered species,Nuphar submersa,based on genotype data of all remnant individuals growing in the wild International conference

    Shiga T, M.Yokogawa, S.Kaneko, Y.Isagi

    East Asian Plant Diversity and Conservation 2016  2016.8  East Asian Plant Diversity and Conservation

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    Venue:Tokyo  

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  • 博物館に収蔵されているカヤツリグサ科植物種子の発芽可能性

    志賀 隆, 平澤優輝, 長谷川匡広

    すげの会第27回全国大会  2016.6  すげの会

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    Venue:阿賀  

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  • 標本作製・保存方法による種子生存率の経年変化

    平澤優輝, 港 翼, 志賀 隆

    日本植物分類学会第15回大会  2016.3  日本植物分類学会

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    Venue:富山  

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  • 絶滅危惧水生植物オグラコウホネの系統地理学的研究

    小林美雨, 厨子雅人, 光田重幸, Choi H, 志賀 隆

    日本植物分類学会第15回大会  2016.3  日本植物分類学会

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    Venue:富山  

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  • 重要生態系監視地域モニタリング推進事業「モニタリングサイト1000」における水生植物調査の始動

    加藤 将, 横井謙一, 角野康郎, 國井秀伸, 志賀 隆, 西廣 淳, 山ノ内崇志, 高村典子, 川越久史, 最上祥成, 宮田 亮

    水草研究会全国集会  2016  水草研究会

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    Venue:高知  

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  • 夜沼(岩手県八幡平市)におけるカラフトグワイの発見:生育環境と近隣地域集団との遺伝的関係

    志賀 隆, 加藤 将, 薄葉 満, 山ノ内崇志, 倉園知広, 平澤優輝, 山崎真実

    水草研究会全国集会  2016  水草研究会

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    Venue:高知  

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  • Genetic identification of traded endangered plants based on genotype data of all remnant individuals glowing in the wild International conference

    Shiga T, M.Yokogawa, T.Kaneko, Y.Isagi

    The 7th EAFES International Congress  2016  EAFES

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    Venue:Daegu  

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  • 絶滅危惧種シモツケコウホネに対して取り組まれた保全事業の評価

    矢口隼人, 志賀 隆

    日本陸水学会甲信越支部会第41回大会  2015.11  日本陸水学会甲信越支部

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    Venue:新発田  

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  • 標本作製・保存方法の違いによる種子生存率の変化

    平澤優輝, 港 翼, 志賀 隆

    日本陸水学会甲信越支部会第41回大会  2015.11  日本陸水学会甲信越支部

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    Venue:新発田  

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  • 絶滅危惧水生植物オグラコウホネの系統地理学的研究

    小林美雨, 厨子雅人, 光田重幸, Choi H, 志賀 隆

    日本陸水学会甲信越支部第41回大会  2015.11  日本陸水学会甲信越支部

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    Venue:新発田  

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  • 標本作製・保存方法・時間経過が種子生存に与える影響

    平澤優輝, 港 翼, 志賀 隆

    日本植物分類学会第14回大会  2015.3  日本植物分類学会

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    Venue:福島  

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  • その標本のタネ、生きてますよ!:標本種子を用いた絶滅集団復元の試み

    志賀 隆

    日本植物分類学会第14回大会公開シンポジウム  2015.3  日本植物分類学会

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    Venue:福島  

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  • 日本にトゲホザキノフサモは生育するのか?

    志賀 隆

    水草研究会全国集会  2015  水草研究会

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    Venue:福島  

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  • 押し花標本のタネから芽が出る?

    志賀 隆

    企画展「めがはじまり~樹木の実生・命のものがたり~」関連講座  2015  長岡市立科学博物館

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    Venue:長岡  

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  • 日本の水草を調べる・分類する・守る

    志賀 隆

    東北植物研究会2015年度講演会  2015  東北植物研究会

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    Venue:盛岡  

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  • 時間経過にともなう標本種子の生存率の変化:標本作製・管理方法に注目して

    平澤優輝, 港 翼, 志賀 隆

    日本陸水学会甲信越支部会第40回大会  2014.11  日本陸水学会甲信越支部

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    Venue:安曇野  

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  • 福島潟における埋土種子集団の構成:現地調査と撒き出し試験から

    渡辺雄大, 志賀 隆

    日本陸水学会甲信越支部会第40回大会  2014.11  日本陸水学会甲信越支部

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    Venue:安曇野  

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  • 標本の種子は長生きか?

    平澤優輝, 港 翼, 長谷川匡弘, 志賀 隆

    日本植物学会第78回大会  2014.9  日本植物学会

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    Venue:川崎  

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  • 身近な植物をどのように守るか~シモツケコウホネを例にしてから Invited

    志賀 隆

    うじいえ自然に親しむ会記念講演会  2014.5  うじいえ自然に親しむ会

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    Venue:さくら  

    さくら市ミュージアム講座室で定期総会の後に行われました。講師はシモツケコウホネの名付け親である、志賀隆新潟大学教育学部准教授でした。日光市小代のシモツケコウホネを例にして、これからどのようにして、保全するか私達に宿題が出されました。
    参加者は、さくら市、日光市、宇都宮市、小山市、那須烏山市、塩谷町、高根沢町などから55名でした。

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  • 時を超えて発芽する博物館標本のタネ~標本種子を用いた絶滅植物の復元~

    志賀 隆

    大阪市立自然史博物館自然史オープンセミナー  2014.3  大阪市立自然史博物館

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    Venue:大阪  

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  • 種子の生存率が高い標本作製・管理方法の検討

    平澤優輝, 港 翼, 志賀 隆

    日本植物分類学会第13回大会  2014.3  日本植物分類学会

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    Venue:熊本  

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  • タネツケバナ属ナガエジャニンジンの分類学的再検討

    阿部知美, 工藤 洋, 志賀 隆

    日本植物分類学会第13回大会  2014.3  日本植物分類学会

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    Venue:熊本  

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  • シモツケコウホネの生育地の現状:生育環境と形質の関係

    志賀 隆, 五十嵐あすか, 柴田由子

    水草研究会全国集会  2014  水草研究会

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    Venue:島根  

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  • First record of Myriophyllum oguraense Miki (Haloragaceae) in Korea International conference

    Choi H.J, Jang J, T.Shiga

    The 46th Annual Meeting of Korean Society of Plant Taxonomists  2014  Korean Society of Plant Taxonomists

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    Venue:Daejeon  

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  • 博物館標本種子を用いて絶滅危惧種野生集団の遺伝的多様性を回復させることは可能か?

    中浜直之, 平澤優輝, 港 翼, 井鷺裕司, 志賀 隆

    第45回種生物学シンポジウム  2013.11  種生物学会

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    Venue:別府  

    ポスター発表賞受賞

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  • ジャニンジンとナガエジャニンジンは別種か?-形態形質と分子データに基づく検討ー

    阿部知美, 志賀 隆

    日本陸水学会甲信越支部会第39回研究発表会  2013.11  日本陸水学会甲信越支部

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    Venue:北杜  

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  • 標本種子の発芽・生存率と標本作製・管理方法の関係

    平澤優輝, 港 翼, 長谷川匡弘, 志賀 隆

    日本陸水学会甲信越支部会第39回研究発表会  2013.11  日本陸水学会甲信越支部

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    Venue:北杜  

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  • 絶滅危惧水生植物シモツケコウホネの生育環境と群落衰退要因の解明

    五十嵐あすか, 志賀 隆

    日本陸水学会甲信越支部会第39回研究発表会  2013.11  日本陸水学会甲信越支部

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    Venue:北杜  

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  • どのような博物館標本の種子が生存しているのか?

    港 翼, 長谷川匡弘, 志賀 隆

    日本植物分類学会第12回大会  2013.3  日本植物分類学会

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    Venue:千葉  

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  • どのような種や状態の博物館標本の種子が生きているのか?

    志賀 隆, 港 翼, 長谷川匡弘

    日本生態学会第60回大会  2013.3  日本生態学会

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    Venue:静岡  

    博物館の標本庫には100年以上前のものから現在に至るまで、数多くの植物標本が収められており、都市化などにより現在では失われてしまった集団の標本も残されている。このような標本から種子や胞子を採集し、撒きだすことによって失われた集団を復元したり、遺伝的に劣化した集団の遺多様性を回復させたりすることができる可能性がある。そこで、本研究では標本の種子から集団を復元することが可能な種をリストし、その状態を明らかにするために、発芽試験と種子の染色試験を行った。
    調査対象種は絶滅危惧植物を中心に選び、大阪市立自然史博物館(OSA)において81種255点の標本から種子・胞子を得た。発芽試験は段階温度法を用いて行い、未発芽種子はテトラゾリウム1%溶液に浸けて胚の酵素活性の有無を確認した。
    発芽試験の結果、18種33点の標本種子からの発芽が確認できた(1番古いものは1989年採集のミヤコグサ)。また、テトラゾリウム染色試験によって未発芽種子の胚が赤色に呈色し、酵素活性があった標本は47種74点であった(1番古いものは1925年採集のヒメヒゴタイ)。未発芽の生存種子は発芽条件を変えたり、植物ホルモンを添加したり、生存組織を培養することにより、発芽実生や成長した植物体を得ることができる可能性がある。発芽や呈色反応が確認された標本は採集年からの時間経過とともに減少した。発芽もしくは呈色反応が確認され、種子が生存していると判断できたものは合計53種(全種の65%)、107点(全標本の42%)であった。
    以上のことより、標本種子を用いて失われた集団を復元することは種によっては可能であり、標本庫に収められている植物標本は野生植物のシードバンクとして重要な役割を果たせると結論した。なお、現在も発芽試験を継続しており、これらの結果についても報告する予定である。

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  • 博物館標本の種子を用いた植物集団の復元は可能か?

    港 翼, 長谷川匡弘, 志賀 隆

    日本陸水学会甲信越支部会第38回研究発表会  2012.12  日本陸水学会甲信越支部

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    Venue:茅野  

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  • 博物館標本の種子は生きている?:標本種子を用いた絶滅集団復元の試み

    志賀 隆

    日本植物分類学会第11回大会  2012.3  日本植物分類学会

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    Venue:大阪  

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  • 絶滅危惧水生植物オグラコウホネ Nuphar oguraensisの遺伝的変異

    厨子雅人, 志賀 隆, 光田重幸

    日本植物分類学会第11回大会  2012.3  日本植物分類学会

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    Venue:大阪  

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  • プロジェクトY~みんなで自然を調べよう 淀川・大和川自然調査プロジェクト9年間の軌跡~

    志賀 隆

    日本植物分類学会第11回大会公開シンポジウム  2012.3  日本植物分類学会

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    Venue:大阪  

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  • 標本の利用について:特に種子の利用に注目して

    志賀 隆

    大阪市立自然史博物館自然史オープンセミナー  2012  大阪市立自然史博物館

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    Venue:大阪  

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  • 全個体遺伝子型解析による絶滅危惧植物の保全

    井鷺裕司, 兼子信吾, 水谷未那, 加藤慶子, 伊津野彩子, 高宮正之, 志賀 隆, 増本育子, 大竹邦明

    日本DNA多型学会第20回学術集会  2011.12  日本DNA多型学会

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    Venue:横浜  

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  • Conservation of critically endangered plant species based oninformation obtained by the ubiquitous genotyping International conference

    Isagi Y, S.Kaneko, K.Kato, A.Izuno, M.Mizutani, T.Shiga, I.Masumoto, K.Ohtake

    18th International Botanical Congress  2011.7  International Botanical Congress

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    Venue:Melbourne  

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  • Genetic diversity and population structure of Nuphar submersa(Nymphaeaceae),an endangered aquatic plant endemic to Japan,and its conservation implications International conference

    Shiga T, M.Yokogawa, S.Kaneko, Y.Isagi

    East Asian Botany,International Symposium 2011 and 10th Annual Meeting of the Japanese Society of Plant Systematics  2011.3  East Asian Botany

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    Venue:Tsukuba  

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  • 地域固有絶滅危惧種シモツケコウホネを守る:ユビキタスジェノタイピングから見えてきたこと

    志賀 隆, 横川昌史, 兼子信吾, 井鷺裕司

    日本生態学会第58回大会  2011.3  日本生態学会

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    Venue:札幌  

    シモツケコウホネNuphar submersa Shiga & Kadono(スイレン科)は2006年に新種として発表された栃木県固有の絶滅危惧水生植物である(絶滅危惧ⅠA類)。栃木県中部の河川や水路に生育していたが、圃場整備などによってそのほとんどは絶滅し、現在では3地域(日光市、那須烏山市、真岡市)に群落面積にして合計60㎡残っているに過ぎない。そのような中、生育面積が最も広い日光市では、2009年に移植による系統保存と現状維持地区を設け、保全に配慮した形で圃場整備が実施されることになった。そこで発表者らは有効な保全策の提案を目的に、現存する株の網羅的な遺伝子型決定(ユビキタスジェノタイピング)を行い、各集団の個体数や遺伝的多様性を評価した。
    サンプルは、0.5m間隔で採集し(日光市202株、那須烏山市58株、真岡市7株)、マイクロサテライトマーカー12遺伝子座を用いて遺伝子型を決定した。その結果、3集団とも遺伝子座あたりの対立遺伝子数1.6~1.9、ヘテロ接合度0.11~0.42と低い遺伝的多様性を示したものの、日光市27個体、那須烏山市15個体、真岡市3個体の合計45個体を識別することができ、遺伝子型多様性は集団内に維持されていることが明らかになった。また、各集団はそれぞれ同程度、遺伝的に分化しており(Da=0.38~0.44)、集団サイズが小さく個体数が少ない真岡市の集団も系統的に重要であることが明らかになった。
    日光市での圃場整備を遺伝的多様性の面から評価すると、現状維持地区を設けたことにより20個体を残すことができた反面、残念ながら移植によって複数個体が失われていた。このようにユビキタスジェノタイピングは保全事業のチェック方法としても有効であった。この他にも現地での保全活動について紹介し、ユビキタスジェノタイピングに基づく保全活動の有効性を議論したい。

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  • ムラサキトキンソウ(仮称)の新産地と生育状況

    赤井賢成, 武浪秀子, 沢和 浩, 杉山多喜子, 葛西英明, 久米 修, 志賀 隆, 小川 誠, 矢野興一, 池田 博, 吉岡俊人

    日本雑草学会第50回大会  2011  日本雑草学会

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    Venue:東京  

    発表延期

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  • しぜんと友だちになりたくて Invited

    志賀 隆

    県下生物標本展示会第60回記念公演会  2011  長岡市立科学博物館

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    Venue:長岡  

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  • 遺伝子解析から見いだされたコウホネ属の絶滅危惧種~見逃されてきた絶滅危惧種とその保全~

    志賀 隆, 横川昌史, 兼子信吾, 井鷺裕司

    日本植物学会第74回大会  2010.9  日本植物学会

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    Venue:春日井  

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  • 淀川水系における外来水草ボタンウキクサの生活史

    武林周一郎, 志賀 隆, 淀川水系調査グループ, プロジェクトY植物班

    水草研究会全国集会第32回大会  2010.8  水草研究会

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    Venue:大阪  

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  • 大阪北部におけるカワヂシャと外来植物オオカワヂシャおよび雑種の分布

    志賀 隆, 淀川水系調査グループ, プロジェクトY植物班

    水草研究会全国集会第32回大会  2010.8  水草研究会

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    Venue:大阪  

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  • 水生植物ナガレコウホネ(スイレン科)の携帯変異と遺伝的変異

    志賀 隆, 杉田勇治, 横川昌史, 兼子信吾, 井鷺裕司

    日本植物分類学会第9回大会  2010.3  日本植物分類学会

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    Venue:刈谷  

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  • 淀川水系における水生植物の分布状況

    志賀 隆, 大阪市立自然史博物館淀川水系調査グループ植物班

    関西自然保護機構2010年度大会  2010.3  関西自然保護機構

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    Venue:大阪  

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  • 水生植物コウホネ属(スイレン科)における「種」のあり方について Invited

    志賀 隆

    近畿植物同好会総会  2010  近畿植物同好会

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    Venue:大阪  

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  • シモツケコウホネはどのような水草なのか.シモツケコウホネを知る・守る Invited

    志賀 隆

    栃木県立博物館・京都大学農学研究科・環境省の三者による共催シンポジウム  2010 

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    Venue:宇都宮  

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  • 淀川水系における外来水生植物の分布状況

    志賀 隆, 大阪市立自然史博物館淀川水系調査グループ植物班

    水草研究会全国集会第31回大会  2009  水草研究会

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    Venue:山形  

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  • コウホネ属(スイレン科)の自然史 Invited

    志賀 隆

    水草研究会全国集会第31回大会  2009  水草研究会

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    Venue:山形  

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  • 琵琶湖におけるミズヒマワリの侵入とその現状について

    藤井伸二, 志賀 隆, 金子有子, 栗林 実, 野間直彦

    水草研究会全国集会第30回大会  2008  水草研究会

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    Venue:千葉  

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  • 水生植物コウホネ(スイレン科)の系統地理および形態的変異

    志賀 隆, 井鷺裕司, 角野康郎

    日本植物分類学会第6回大会  2007  日本植物分類学会

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    Venue:新潟  

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  • 日本のコウホネ属(スイレン科)は分類できるのか?

    志賀 隆

    平成18年度日本植物分類学会講演会  2006  日本植物分類学会

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    Venue:大阪  

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  • シモツケコウホネはなぜ新種なのかー新種発見に至るまでーコウホネの里

    志賀 隆

    連続・地域資源講座(第3回)  2006 

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    Venue:日光  

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  • コウホネ属新変種シモツケコウホネの発見:産地の現状と保全の重要性

    志賀 隆, 石井 潤, 角野康郎

    水草研究会全国集会第27回大会  2005  水草研究会

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    Venue:米子  

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  • 水生植物コウホネ属の種間交雑:コウホネとネムロコウホネの場合

    志賀 隆, 井鷺裕司, 角野康郎

    日本植物分類学会第4回大会  2005  日本植物分類学会

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    Venue:高知  

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  • 立地条件が規定するヨシ群落の遺伝構造ー群落の遺伝的多様性維持に関わる小規模水系の機能

    井鷺裕司, 近藤俊明, 志賀 隆, 金子有子

    日本生態学会第51回大会  2004.8  日本生態学会

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    Venue:釧路  

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  • 日本産コウホネ属の浸透性交雑とヒメコウホネ(狭義)の現状

    志賀 隆, 井鷺裕司, 角野康郎

    日本植物分類学会第3回大会  2004  日本植物分類学会

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    Venue:東広島  

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  • 日本産コウホネ属の分類学的問題点:本当に分類できるのか?

    志賀 隆, 角野康郎

    水草研究会全国大会第26回大会  2004  水草研究会

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    Venue:秋田  

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  • 表土を戻すことは畦畔植生の保全と復元にどこまで有効か?

    武林周一郎, 志賀 隆, 角野康郎

    日本生態学会第50回大会  2003.3  日本生態学会

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    Venue:つくば  

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  • ヒメコウホネおよび近縁種の分類学的再検討

    志賀 隆, 角野康郎

    日本植物分類学会第2回大会  2003  日本植物分類学会

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    Venue:神戸  

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  • 日本産コウホネ属の形態変異と遺伝的変異

    志賀 隆

    第35回種生物学シンポジウム  2003  種生物学会

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    Venue:神戸  

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  • 日本産コウホネ類の分類は可能か?-兵庫県産コウホネ類を中心にー

    志賀 隆

    水辺ネットワーク2003年度交流会  2003  水辺ネットワーク

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    Venue:姫路  

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  • ヒメコウホネ2型の分類学的再検討

    志賀 隆, 角野康郎

    日本植物分類学会第1回大会  2002  日本植物分類学会

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    Venue:東京  

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  • 新潟市松浜の地(阿賀野川河口)の底生動物概観

    志賀 隆, 田中啓太, 川島慎一, 帆苅信夫, 富樫繁春, 安部信之, 伊藤 章, 伊藤正一, 松本史郎, 馬場吉弘, 本間義治

    日本陸水学会甲信越支部会第26回研究発表会  2000  日本陸水学会甲信越支部会

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    Venue:松本  

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Works

Research Projects

  • 淡水藻類シャジクモの生態的2型における生態的種分化の実証的研究

    Grant number:23K05903

    2023.4 - 2026.3

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    加藤 将, 坂山 英俊, 志賀 隆

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

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  • Comprehensive evaluation of germinabilities of seeds of herbarium specimens based on multi storage facilities and exhaustive taxon sampling.

    Grant number:21H00620

    2021.4 - 2024.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

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    Grant amount:\17420000 ( Direct Cost: \13400000 、 Indirect Cost:\4020000 )

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  • 博物館標本胞子を用いた絶滅集団の復元:簡易生存識別法と標本管理法の開発

    Grant number:20K20715

    2020.7 - 2023.3

    System name:科学研究費助成事業 挑戦的研究(萌芽)

    Research category:挑戦的研究(萌芽)

    Awarding organization:日本学術振興会

    志賀 隆, 加藤 将

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    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    シダ植物の博物館標本胞子の生存可能性を評価するために、昨年度標本胞子の発芽試験を行った種に加えて、新たにスギナ(トクサ科)、オオハナワラビ(ハナワラビ科)の2種を研究対象種に加えた。2021年度は、大阪市立自然史博物館と新潟大学の植物標本庫に収蔵されている標本よりそれぞれ胞子(以下、標本胞子と呼ぶ)を新たに得た。
    また、胞子の生理活性を評価するために、オオハナワラビ20標本、スギナ18標本、ゼンマイ19標本に標本胞子に対して、MTTを用いたテトラゾリウム染色試験を行った。オオハナワラビは、標本作成からの経過時間が183ヶ月(15年)の胞子の生存率が85.5±1.7%と最も高く、446ヶ月(38年)経過した胞子の生存率が37.9±4.2%と2番目に高かった。それ以外の年代の胞子は生存率が10%を下回っており、6標本においては0%であった。スギナやゼンマイでは、標本作成からの経過時間が30年から40年にかけて生存率は緩やかに減少した。しかし、各種の幾つかの古い標本では過剰に染色される胞子も確認され、胞子の生存を過剰評価している可能性が考えられた。
    車軸藻類については、昨年度に続き野外採集を行い発芽試験のための標本を増強するとともに、昨年度と今年度作製したシャジクモの標本(群馬県水田産1集団、新潟県ため池産2集団)より卵胞子を採取し、発芽試験(標本卵胞子およびコントロールデータのための非標本卵胞子を各50粒播種、6反復実施)を行った。その結果、新潟県産2集団の標本卵胞子において発芽が認められた(0.3±0.82%~2.7±0.01%)。これまでの研究で卵胞子に乾燥耐性があることは一部の種で示されていたが、標本の卵胞子に着目して発芽を確認したのは本研究が初めてである。今後、車軸藻類においても博物館標本の利用可能性を検討する価値があることを示すデータが得られた。

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  • 湿地の多面的価値評価軸の開発と広域評価に向けた情報基盤形成

    2017.4 - 2020.3

    System name:環境研究総合推進費

    Awarding organization:環境省

    西廣 淳

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    Grant type:Competitive

    サブグループリーダー

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  • 新潟市域の湖沼に生育する水生植物の遺伝的特徴と埋土種子集団の構成

    2017.4 - 2018.3

    System name:受託研究

    Awarding organization:新潟県新潟市

    志賀 隆

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  • 新潟市域の湖沼における水生湿生植物相と埋土種子集団の構成

    2015.4 - 2017.3

    System name:受託研究

    Awarding organization:新潟県新潟市

    志賀 隆

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  • 頸城湖沼群の水生植物相調査

    2015.4 - 2016.3

    System name:寄付金

    Awarding organization:日本国際湿地保全連合

    志賀 隆

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  • 博物館植物標本の生存組織を用いた絶滅集団の復元:組織培養法の確立と普及

    2014.4 - 2017.3

    System name:科学研究費補助金(基盤C)

    Awarding organization:文部科学省

    志賀 隆

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4810 ( Direct Cost: \3700 、 Indirect Cost:\1110 )

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  • 福島潟の植生の現状と埋土種子集団の構成

    2014.4 - 2015.3

    System name:受託研究

    Awarding organization:新潟県新潟市

    志賀 隆

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  • 「シモツケコウホネ」保全調査研究

    2012.9 - 2020.3

    System name:受託研究

    Awarding organization:栃木県日光市

    志賀 隆

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  • Creation of support basis for amateur-mycology through natural history museum as information provider and trial piece of local excicata with genetic reference.

    Grant number:23300333

    2011.4 - 2015.3

    System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    SAKUMA Daisuke, DEGAWA Yousuke, HASHIYA Makoto, SHIGA Takashi

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    Grant amount:\20150000 ( Direct Cost: \15500000 、 Indirect Cost:\4650000 )

    On mycology, research activities of citizen scientists have great importance. We tried to enhance the function of natural history museums as support basis of their activities. 1. We made database of Dr. Hongo's mycological collections, and digitized their images, for utilize them for educational tools and materials for modern studies of both taxonomy and ecology. We have got some important results by our research partners in Boletaceae and Amanitaceae. 2. We conducted many scientific seminars for citizen scientists, and we supported series of mycological exhibitions of local museums. 3. We have got good results in mycological DNA barcoding from modern samples and, partly from old Hongo's collection. From these activities, we summarized and reviewed the relation ship between citizen scientists and museums, with special reference to importance of curatorship and open database .

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  • Restoration of extinct plant population using the seed of herbarium specimens and development of specimen management method

    Grant number:23701024

    2011.4 - 2014.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Young Scientists (B)

    Awarding organization:Japan Society for the Promotion of Science

    SHIGA Takashi

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\3640 ( Direct Cost: \2800 、 Indirect Cost:\840 )

    I investigated the enzyme activity of the embryo and germination test of herbarium specimens's seed of endangered plant of 555 specimens of 117 species. As a result, the seeds of 235 specimens (42%) of 73 species (62%) were alive. The oldest survival specimen seed was Saussurea pulchella (Fisch. ex Hornem.) Fisch. that has been collected 87 years ago.
    I alos examined the effect of specimen preparation method has on the viability of the seed. Specimen seeds subjected to drying treatment at a temperature lower than 40 degrres C showed a high survival rate.
    And I compared the genetic diversity of seedling populations of specimens of Vincetoxicum pycnostelma Kitag. and the wild populations from which the specimens were collected. Genetic diversity of the seedlings were significantly lower than that of wild populations, but the alleles which were lost from the wild population were confirmed.

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  • 日本産絶滅危惧水生植物の現状、特に情報の不足する種の実態解明

    2011.4 - 2012.3

    System name:研究助成

    Awarding organization:PRO NATURA FUND

    角野康郎

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    Grant type:Competitive

    研究代表代行者

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  • Evolutionary process of life form and heterophylly in Nuphar Sm. (Nymphaeaceae)

    Grant number:20770074

    2008.4 - 2011.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Young Scientists (B)

    Awarding organization:Japan Society for the Promotion of Science

    SHIGA Takashi

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\3900 ( Direct Cost: \3000 、 Indirect Cost:\900 )

    I investigated morphological, ecological, and genetical features of Nuphar japonica DC., N. oguraensis Miki, and N. submersa Shiga & Kadono to assess evolutionary process of leaf morphology, life form, and heterophylly in the genus Nuphar Sm. (Nymphaeaceae). In crossing experiment, these species were cross compatible, and I created artificial F1 hybrids with each species for the future ecogenomics study.

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  • 淀川水系における水生・湿生植物の分布調査

    2008.4 - 2010.3

    System name:助成金(調査研究開発)

    Awarding organization:花博記念協会

    志賀 隆

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    Authorship:Principal investigator  Grant type:Competitive

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  • Research of natural history in the Yodo River system by citizenry participation and the museum academic meanings

    Grant number:20605021

    2008.4 - 2010.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    NAKAJO Takeshi, ISHIDA So, HATOOKA Kiyotaka, SHIGA Takashi, MASUDA Harue, WADA Takeshi, SHIYAKE Shigehiko, OKADE Tomoko, OHISHI Hisashi

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    Grant type:Competitive

    Grant amount:\4550 ( Direct Cost: \3500 、 Indirect Cost:\1050 )

    We performed environmental researches of the Yodo River system in Osaka in cooperation with civic membership and Friends of the Osaka Museum of Natural History. These actions were promoted the raising awareness of environmental issues and nature conservation for citizen. We accomplished environmental researches and museology achievements through this work among understanding of water quality and fauna in the Yodo River system, environmental protection, promote citizens involved in voluntary environmental problems, the sample data aggregation to the museum, holding of special exhibitions and creating the book, and the results over the Internet publication.

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  • 水生植物コウホネ属(スイレン科)の系統地理学的研究

    2007.4 - 2008.3

    System name:研究助成

    Awarding organization:昭和聖徳記念財団

    志賀 隆

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    Authorship:Principal investigator  Grant type:Competitive

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  • 水生植物シモツケコウホネ(スイレン科)の保全生物学的研究

    2007.4 - 2008.3

    System name:研究奨励金

    Awarding organization:環境科学総合研究所

    志賀 隆

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    Authorship:Principal investigator  Grant type:Competitive

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Teaching Experience

  • 中等教育実習I(事前・事後指導)

    2023
    Institution name:新潟大学

  • 初等教育実習I(事前・事後指導)

    2023
    Institution name:新潟大学

  • 初等教育実習II(事前・事後指導)

    2023
    Institution name:新潟大学

  • 自然環境科学 II

    2022
    Institution name:新潟大学

  • 保育内容指導法(環境)

    2021
    Institution name:新潟大学

  • 保育内容の研究(環境)

    2021
    -
    2022
    Institution name:新潟大学

  • 自然科学総論Ⅴ

    2020
    Institution name:新潟大学

  • 生活

    2015
    -
    2017
    Institution name:新潟大学

  • 保全生物学特論Ⅰ

    2014
    Institution name:新潟大学

  • 生態学要論

    2014
    Institution name:新潟大学

  • 保育内容の研究(環境)

    2014
    -
    2022
    Institution name:新潟大学

  • スタディ・スキルズI

    2013
    Institution name:新潟大学

  • 水界生態学特論

    2013
    Institution name:新潟大学

  • 生物学演習B

    2012
    Institution name:新潟大学

  • 植物学

    2012
    Institution name:新潟大学

  • 卒業研究

    2012
    Institution name:新潟大学

  • 基礎生物学実験

    2012
    Institution name:新潟大学

  • 生物学実験II

    2012
    Institution name:新潟大学

  • 基礎生物学A

    2012
    Institution name:新潟大学

  • 生物学演習A

    2012
    Institution name:新潟大学

  • 植物野外実習

    2012
    Institution name:新潟大学

  • 生物学実験I

    2012
    Institution name:新潟大学

  • 生態学演習Ⅰ

    2012
    -
    2017
    Institution name:新潟大学

  • 生物学課題研究III

    2012
    -
    2015
    Institution name:新潟大学

  • 生物学課題研究IV

    2012
    -
    2015
    Institution name:新潟大学

  • 生態学演習Ⅱ

    2012
    -
    2014
    Institution name:新潟大学

  • 生物学課題研究I

    2012
    -
    2014
    Institution name:新潟大学

  • 生物学課題研究II

    2012
    -
    2014
    Institution name:新潟大学

  • 生態学特論Ⅰ

    2012
    -
    2014
    Institution name:新潟大学

  • 水界生態学演習I

    2012
    -
    2013
    Institution name:新潟大学

  • 水界生態学演習II

    2012
    -
    2013
    Institution name:新潟大学

  • 生活環境生物学実習

    2012
    -
    2013
    Institution name:新潟大学

  • 理科教育法(中等)IV

    2012
    Institution name:新潟大学

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Social Activities

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