Faculty of Medicine School of Medicine Assistant Professor
Graduate School of Medical and Dental Sciences Molecular and Cellular Medicine Signal Transduction Research Assistant Professor
Updated on 2024/10/21
博士(医学) ( 2013.4 大阪大学 )
神経
リン酸化プロテオミクス
結晶構造解析
発生
細胞生物
Life Science / Neuroscience-general
Life Science / Structural biochemistry
Life Science / Cell biology
Shimane University Assistant Professor
2024.10
Niigata University Faculty of Medicine School of Medicine Assistant Professor
2013.4
Niigata University Graduate School of Medical and Dental Sciences Molecular and Cellular Medicine Signal Transduction Research Assistant Professor
2013.4
Identification of the growth cone as a probe and driver of neuronal migration in the injured brain. International journal
Chikako Nakajima, Masato Sawada, Erika Umeda, Yuma Takagi, Norihiko Nakashima, Kazuya Kuboyama, Naoko Kaneko, Satoaki Yamamoto, Haruno Nakamura, Naoki Shimada, Koichiro Nakamura, Kumiko Matsuno, Shoji Uesugi, Nynke A Vepřek, Florian Küllmer, Veselin Nasufović, Hironobu Uchiyama, Masaru Nakada, Yuji Otsuka, Yasuyuki Ito, Vicente Herranz-Pérez, José Manuel García-Verdugo, Nobuhiko Ohno, Hans-Dieter Arndt, Dirk Trauner, Yasuhiko Tabata, Michihiro Igarashi, Kazunobu Sawamoto
Nature communications 15 ( 1 ) 1877 - 1877 2024.3
Very-long-chain fatty acids are crucial to neuronal polarity by providing sphingolipids to lipid rafts. International journal
Atsuko Honda, Motohiro Nozumi, Yasuyuki Ito, Rie Natsume, Asami Kawasaki, Fubito Nakatsu, Manabu Abe, Haruki Uchino, Natsuki Matsushita, Kazutaka Ikeda, Makoto Arita, Kenji Sakimura, Michihiro Igarashi
Cell reports 113195 - 113195 2023.10
Correction to: JNK1‑Dependent Phosphorylation of GAP‑43 Serine 142 is a Novel Molecular Marker for Axonal Growth. International journal
Masayasu Okada, Yosuke Kawagoe, Toshiyuki Takasugi, Motohiro Nozumi, Yasuyuki Ito, Hayato Fukusumi, Yonehiro Kanemura, Yukihiko Fujii, Michihiro Igarashi
Neurochemical research 47 ( 9 ) 2683 - 2683 2022.9
JNK1-Dependent Phosphorylation of GAP-43 Serine 142 is a Novel Molecular Marker for Axonal Growth. International journal
Masayasu Okada, Yosuke Kawagoe, Toshiyuki Takasugi, Motohiro Nozumi, Yasuyuki Ito, Hayato Fukusumi, Yonehiro Kanemura, Yukihiko Fujii, Michihiro Igarashi
Neurochemical research 47 ( 9 ) 2668 - 2682 2022.3
Phosphorylation sites of microtubule-associated protein 1B (MAP 1B) are involved in axon growth and regeneration. Reviewed International journal
Ishikawa Y, Okada M, Honda A, Ito Y, Tamada A, Endo N, Igarashi M
Molecular brain 12 ( 1 ) 93 - 93 2019.11
Growth Cone Phosphoproteomics Reveals that GAP-43 Phosphorylated by JNK Is a Marker of Axon Growth and Regeneration. Reviewed International journal
Asami Kawasaki, Masayasu Okada, Atsushi Tamada, Shujiro Okuda, Motohiro Nozumi, Yasuyuki Ito, Daiki Kobayashi, Tokiwa Yamasaki, Ryo Yokoyama, Takeshi Shibata, Hiroshi Nishina, Yutaka Yoshida, Yukihiko Fujii, Kosei Takeuchi, Michihiro Igarashi
iScience 4 190 - 203 2018.6
Glycoprotein M6a as a signaling transducer in neuronal lipid rafts. Reviewed
Ito Y, Honda A, Igarashi M
Neuroscience research 128 19 - 24 2018.3
Extracellular Signals Induce Glycoprotein M6a Clustering of Lipid Rafts and Associated Signaling Molecules Reviewed
Atsuko Honda, Yasuyuki Ito, Kazuko Takahashi-Niki, Natsuki Matsushita, Motohiro Nozumi, Hidenori Tabata, Kosei Takeuchi, Michihiro Igarashi
JOURNAL OF NEUROSCIENCE 37 ( 15 ) 4046 - 4064 2017.4
The four-transmembrane protein IP39 of Euglena forms strands by a trimeric unit repeat Reviewed
Hiroshi Suzuki, Yasuyuki Ito, Yuji Yamazaki, Katsuhiko Mineta, Masami Uji, Kazuhiro Abe, Kazutoshi Tani, Yoshinori Fujiyoshi, Sachiko Tsukita
NATURE COMMUNICATIONS 4 1766 2013.4
Phosphodiesterase isozymes involved in regulating acid secretion in the isolated mouse stomach. Reviewed
S. Okuda, M. Honda, Y. Ito, E. Aihara, S. Kato, S. Mitsufuji, T. Yoshikawa, K. Takeuchi
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 60 Suppl 7 183 - 190 2009.12
Role of endothelial nitric oxide synthase in aggravation of indomethacin-induced gastric damage in adjuvant arthritic rats Reviewed
Shinichi Kato, F. Ohkawa, Y. Ito, K. Amagase, K. Takeuchi
Journal of Physiology and Pharmacology 60 ( 4 ) 147 - 155 2009.12
Dual role of nitric oxide in gastric hypersecretion in the distended stomach: Inhibition of acid secretion and stimulation of pepsinongen secretion Reviewed
Yasuyuki Ito, Sayaka Okuda, Fumikazu Ohkawa, Shinichi Kato, Shoji Mitsufuji, Toshikazu Yoshikawa, Koji Takeuchi
LIFE SCIENCES 83 ( 25-26 ) 886 - 892 2008.12
Increased susceptibility of small intestine to NSAID-provoked ulceration in rats with adjuvant-induced arthritis: Involvement of enhanced expression of TLR4 Reviewed
Shinichi Kato, Yasuyuki Ito, Hikaru Nishio, Yoko Aoi, Kikuko Amagase, Koji Takeuchi
LIFE SCIENCES 81 ( 16 ) 1309 - 1316 2007.9
定量リン酸化プロテオーム解析が明らかにした末梢神経再生時のGAP-43の新規リン酸化部位
岡田 正康, 河嵜 麻実, 伊藤 泰行, 吉田 豊, 藤井 幸彦, 五十嵐 道弘
JSBMS Letters 42 ( Suppl. ) 88 - 88 2017.8
Electron Crystallography of Euglenoid Four-Transmembrane Protein Revealed the Linear Polymerization by a Combination of Three-Ways of Intermolecular Interaction
Hiroshi Suzuki, Yasuyuki Ito, Kazutoshi Tani, Yuji Yamazaki, Masami Uji, Katsuhiko Mineta, Sachiko Tsukita, Yoshinori Fujiyoshi
BIOPHYSICAL JOURNAL 104 ( 2 ) 42A - 42A 2013.1
Pathogenic Importance of Endothelial Isozyme of Nitric Oxide Synthase (eNOS) in Aggravation of Gastric Lesions Induced By Indomethacin and Cold-Restraint Stress in Adjuvant-Induced Arthritic Rats
Shinichi Kato, Yasuyuki Ito, Fumikazu Ohkawa, Koji Takeuchi
GASTROENTEROLOGY 136 ( 5 ) A691 - A691 2009.5
Detrimental role of endothelial nitric oxide synthase (eNOS/NNOS3) in aggravation of indomethacin-induced gastric damage in adjuvant arthritis rats
Shinichi Kato, Yasuyuki Ito, Fumikazu Ohkawa, Kikuko Amagase, Koji Takeuchi
GASTROENTEROLOGY 134 ( 4 ) A220 - A220 2008.4
胎生期の神経幹細胞分裂におけるリン酸化タウの役割
Grant number:23K05975
2023.4 - 2026.3
System name:科学研究費助成事業
Research category:基盤研究(C)
Awarding organization:日本学術振興会
伊藤 泰行
Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )
内因性リン酸化タウを認識する極小分子プローブの開発
2022.8 - 2023.3
System name:令和4年度 新潟大学U-go グラント
伊藤 泰行
Authorship:Principal investigator
脳発生過程で最も高頻度にチロシンリン酸化されるMAP1Bの機能解明
2021.9 - 2024.4
System name:医学系研究奨励
Awarding organization:武田科学振興財団
伊藤 泰行
Authorship:Principal investigator
極長鎖脂肪酸産生による神経軸索成長の制御機構の解明
Grant number:21K06390
2021.4 - 2024.3
System name:科学研究費助成事業
Research category:基盤研究(C)
Awarding organization:日本学術振興会
本多 敦子, 野住 素広, 伊藤 泰行
Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )
申請者はこれまでに、神経成長円錐の形質膜に脂質マイクロドメイン(脂質ラフト)が多く存在し、そこに集積するシグナル分子の集積・活性化により軸索成長が制御されることを示している。脂質ラフトの形成や機能には極長鎖脂肪酸産生が関与しており、成長円錐には極長鎖脂肪酸産生酵素が多く局在するが、両者の関係性は不明である。そこで申請者は、神経成長円錐での極長鎖脂肪酸産生の、脂質ラフト形成や軸索成長における生理的役割を解明するため、成長円錐に豊富な極長鎖脂肪酸産生酵素 GPSN2に着目し、その欠損マウスを作製・解析を行っている。
オミクス解析により、GPSN2ノックアウトマウス胎仔脳の成長円錐における脂質(アシル鎖長別)産生・代謝の変化をノンターゲット解析したところ、ノックアウトマウスにおいて極長鎖脂肪酸を含む特定の脂質合成が阻害されていた。
極長鎖脂肪酸産生阻害による成長円錐の形態・動態変化を解析するため、超解像度顕微鏡および共焦点顕微鏡を用いて、GPSN2ヘテロマウス胎仔脳神経細胞や、脂肪酸伸長酵素shRNAノックダウン神経細胞の成長円錐のイメージング解析を行ったところ、極長鎖脂肪酸産生阻害による成長円錐の形態・動態異常が認められた。同標本における、成長円錐における脂質ラフトの形成や分布の変化を、蛍光標識脂質ラフトマーカーを用いて解析した。
さらに神経細胞の軸索成長や神経回路形成における変化を細胞、組織レベルで解析するため、成長円錐の形態・動態に変化が見られた細胞における軸索成長の異常をライブイメージングにより解析した。
神経発生過程で最も高頻度にチロシンリン酸化される微小管結合タンパク質 MAP1B の役割
2020.7 - 2021.6
System name:塚田医学奨学会助成金
Awarding organization:新潟大学塚田医学奨学基金
伊藤泰行
Authorship:Principal investigator
高頻度チロシンリン酸化MAP1Bによる神経成長円錐のアクチン骨格制御機構の解明
Grant number:20K15897
2020.4 - 2023.3
System name:科学研究費助成事業 若手研究
Research category:若手研究
Awarding organization:日本学術振興会
伊藤 泰行
Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )
高頻度チロシンリン酸化MAP1Bが担う神経成長円錐のアクチン骨格制御機構の解明
2019.11 - 2020.11
System name:基礎科学研究助成
Awarding organization:住友財団
伊藤 泰行
Authorship:Principal investigator Grant type:Competitive
脳発生過程におけるMAP1Bの高頻度チロシンリン酸化部位の機能解析
2019.4 - 2021.9
System name:藤井節郎記念大阪基礎医学研究奨励会助成金
Awarding organization:藤井節郎記念 大阪基礎医学研究奨励会
伊藤 泰行
Authorship:Principal investigator Grant type:Competitive
高頻度チロシンリン酸化MAP1Bが担う神経成長円錐のアクチン骨格制御機構の解明(生体内機能解析)
2019.4 - 2020.3
System name:日本人若手研究者研究助成金
Awarding organization:中島記念国際交流財団
伊藤 泰行
Authorship:Principal investigator Grant type:Competitive
Roles of the signal transduction via lipid rafts in axon formation and regeneration
Grant number:18K06480
2018.4 - 2021.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
HONDA ATSUKO
Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )
To clarify regulatory mechanisms of the signals for axon formation, we examined the activation system of the signal transduction through the lipid rafts by a membrane protein, M6a in the neuronal growth cones (GC). We analyzed the molecules interacting with M6a on plasma membrane, or the proteins localized in GC lipid rafts via M6a, using proteomic analysis. Our proteomic data showed that M6a interacts with adhesion molecules, e.g. Integrin, the receptors and endoplasmic reticulum on plasma membrane, and recruits some determinants of the neuronal polarity, some kinases, G-proteins and some lipid synthases to lipid rafts. These data indicated that the extra-intra cellular signal transductions and the lipid synthesis in the lipid rafts were intensified by M6a in the GC.
Actin-dependent endocytosis at the leading edge of the growth cone
Grant number:18K06459
2018.4 - 2021.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Nozumi Motohiro
Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )
Growth cones are essential for proper neural circuit formation and axon regeneration. At the leading edge of the growth cone, the plasma membrane is pushed by the elongation of the actin cytoskeleton, which generates the driving force for axon outgrowth. In this study, we found non-adhesive filopodia extending vertically from the surface of the growth cones by three-dimensional observations using super-resolution microscopy, 3D-SIM. The filopodia showed lipid raft-dependent accumulation of neuropilin-1, an axon guidance receptor, and repeated elongation and retraction within a minute. The growth cone could actively recruit receptors toward the extracellular space through the filopodia elongation, suggesting that they efficiently capture soluble ligands.
高頻度チロシンリン酸化MAP1B の神経発生における役割
2018.1 - 2019.4
System name:研究奨励金
Awarding organization:上原記念生命科学財団
伊藤 泰行
Authorship:Principal investigator Grant type:Competitive
脳発生過程に関与するチロシンリン酸化修飾の網羅的解析と意義解明
2016.9 - 2019.5
System name:医学系研究奨励
Awarding organization:武田科学振興財団
伊藤 泰行
Authorship:Principal investigator Grant type:Competitive
Claudin類似4回膜貫通タンパク質Euglena-IP39の構造および機能解析
2011.4 - 2013.3
System name:特別研究員奨励費
Awarding organization:日本学術振興会
伊藤 泰行
Authorship:Principal investigator Grant type:Competitive
分子生物学
生体内物質と代謝