2021/12/01 更新

写真a

イトウ ヤスユキ
伊藤 泰行
ITO Yasuyuki
所属
教育研究院 医歯学系 医学系列 助教
医学部 医学科 助教
医歯学総合研究科 分子細胞医学専攻 シグナル伝達 助教
職名
助教
外部リンク

学位

  • 博士(医学) ( 2013年4月   大阪大学 )

研究キーワード

  • 神経

  • リン酸化プロテオミクス

  • 結晶構造解析

  • 発生

  • 細胞生物

研究分野

  • ライフサイエンス / 神経科学一般

  • ライフサイエンス / 構造生物化学

  • ライフサイエンス / 細胞生物学

経歴

  • 新潟大学   医学部 医学科   助教

    2013年4月 - 現在

  • 新潟大学   医歯学総合研究科 分子細胞医学専攻 シグナル伝達   助教

    2013年4月 - 現在

 

論文

  • Phosphorylation sites of microtubule-associated protein 1B (MAP 1B) are involved in axon growth and regeneration. 査読

    Ishikawa Y, Okada M, Honda A, Ito Y, Tamada A, Endo N, Igarashi M

    Molecular brain   12 ( 1 )   93   2019年11月

  • Growth Cone Phosphoproteomics Reveals that GAP-43 Phosphorylated by JNK Is a Marker of Axon Growth and Regeneration. 査読 国際誌

    Asami Kawasaki, Masayasu Okada, Atsushi Tamada, Shujiro Okuda, Motohiro Nozumi, Yasuyuki Ito, Daiki Kobayashi, Tokiwa Yamasaki, Ryo Yokoyama, Takeshi Shibata, Hiroshi Nishina, Yutaka Yoshida, Yukihiko Fujii, Kosei Takeuchi, Michihiro Igarashi

    iScience   4   190 - 203   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Neuronal growth cones are essential for nerve growth and regeneration, as well as for the formation and rearrangement of the neural network. To elucidate phosphorylation-dependent signaling pathways and establish useful molecular markers for axon growth and regeneration, we performed a phosphoproteomics study of mammalian growth cones, which identified >30,000 phosphopeptides of ∼1,200 proteins. The phosphorylation sites were highly proline directed and primarily MAPK dependent, owing to the activation of JNK, suggesting that proteins that undergo proline-directed phosphorylation mediate nerve growth in the mammalian brain. Bioinformatics analysis revealed that phosphoproteins were enriched in microtubules and the cortical cytoskeleton. The most frequently phosphorylated site was S96 of GAP-43 (growth-associated protein 43-kDa), a vertebrate-specific protein involved in axon growth. This previously uncharacterized phosphorylation site was JNK dependent. S96 phosphorylation was specifically detected in growing and regenerating axons as the most frequent target of JNK signaling; thus it represents a promising new molecular marker for mammalian axonal growth and regeneration.

    DOI: 10.1016/j.isci.2018.05.019

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  • Glycoprotein M6a as a signaling transducer in neuronal lipid rafts. 査読

    Ito Y, Honda A, Igarashi M

    Neuroscience research   128   19 - 24   2018年3月

  • Extracellular Signals Induce Glycoprotein M6a Clustering of Lipid Rafts and Associated Signaling Molecules 査読

    Atsuko Honda, Yasuyuki Ito, Kazuko Takahashi-Niki, Natsuki Matsushita, Motohiro Nozumi, Hidenori Tabata, Kosei Takeuchi, Michihiro Igarashi

    JOURNAL OF NEUROSCIENCE   37 ( 15 )   4046 - 4064   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC NEUROSCIENCE  

    Lipid raft domains, where sphingolipids and cholesterol are enriched, concentrate signaling molecules. Toexaminehowsignaling protein complexes are clustered in rafts, we focused on the functions of glycoprotein M6a (GPM6a), which is expressed at a high concentration in developing mouse neurons. Using imaging of lipid rafts, we found that GPM6a congregated in rafts in a GPM6a palmitoylation-dependent manner, thereby contributing to lipid raft clustering. In addition, we found that signaling proteins downstream of GPM6a, such as Rufy3, Rap2, and Tiam2/STEF, accumulated in lipid rafts in a GPM6a-dependent manner and were essential for laminin-dependent polarity during neurite formation in neuronal development. In utero RNAi targeting of GPM6a resulted in abnormally polarized neurons with multiple neurites. These results demonstrate that GPM6a induces the clustering of lipid rafts, which supports the raft aggregation of its associated downstream molecules for acceleration of neuronal polarity determination. Therefore, GPM6a acts as a signal transducer that responds to extracellular signals.

    DOI: 10.1523/JNEUROSCI.3319-16.2017

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  • The four-transmembrane protein IP39 of Euglena forms strands by a trimeric unit repeat 査読

    Hiroshi Suzuki, Yasuyuki Ito, Yuji Yamazaki, Katsuhiko Mineta, Masami Uji, Kazuhiro Abe, Kazutoshi Tani, Yoshinori Fujiyoshi, Sachiko Tsukita

    NATURE COMMUNICATIONS   4   1766   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Euglenoid flagellates have striped surface structures comprising pellicles, which allow the cell shape to vary from rigid to flexible during the characteristic movement of the flagellates. In Euglena gracilis, the pellicular strip membranes are covered with paracrystalline arrays of a major integral membrane protein, IP39, a putative four-membrane-spanning protein with the conserved sequence motif of the PMP-22/EMP/MP20/Claudin superfamily. Here we report the three-dimensional structure of Euglena IP39 determined by electron crystallography. Two-dimensional crystals of IP39 appear to form a striated pattern of antiparallel double-rows in which trimeric IP39 units are longitudinally polymerised, resulting in continuously extending zigzag-shaped lines. Structural analysis revealed an asymmetric molecular arrangement in the trimer, and suggested that at least four different interactions between neighbouring protomers are involved. A combination of such multiple interactions would be important for linear strand formation of membrane proteins in a lipid bilayer.

    DOI: 10.1038/ncomms2731

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  • Phosphodiesterase isozymes involved in regulating acid secretion in the isolated mouse stomach. 査読

    S. Okuda, M. Honda, Y. Ito, E. Aihara, S. Kato, S. Mitsufuji, T. Yoshikawa, K. Takeuchi

    Journal of physiology and pharmacology : an official journal of the Polish Physiological Society   60 Suppl 7   183 - 190   2009年12月

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    掲載種別:研究論文(学術雑誌)  

    The effect of subtype-selective phosphodiesterase (PDE) inhibitors on acid secretion was examined in mouse stomachs to investigate which PDE isozymes are involved in the local regulation of this secretion. Male DDY mice were used after 18 h fasting. An isolated stomach was incubated in an organ bath containing buffered solution gassed with 95% O(2)/5% CO(2), while the lumen was perfused with unbuffered solution gassed with 100% O(2). Acid secretion was measured at pH 5.4 using a pH-stat method. Histamine or pituitary adenylate cyclase activating polypeptide (PACAP) was added to the serosal solution. PDE inhibitors were added to the serosal solution 30 min before histamine or PACAP. The secretion of acid in the isolated stomach was increased by histamine or PACAP, and these responses were totally inhibited by famotidine. IBMX alone increased basal acid secretion and significantly enhanced the acid responses to histamine and PACAP. Among the PDE inhibitors tested, only rolipram (PDE4 inhibitor) significantly increased basal acid secretion and potentiated the acid responses to histamine and PACAP. The latter peptide increased histamine release into the medium, and this response was also enhanced by rolipram. Furthermore, rolipram significantly increased cAMP production induced in the isolated stomach by histamine and PACAP. These results suggest that PDE4 is involved in the local regulation of gastric acid secretion via the degradation of cAMP and that the PDE4 inhibitor rolipram increases the secretion of acid by potentiating acid production in parietal cells and enhancing histamine release from enterochromaffin-like cells.

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  • Dual role of nitric oxide in gastric hypersecretion in the distended stomach: Inhibition of acid secretion and stimulation of pepsinongen secretion 査読

    Yasuyuki Ito, Sayaka Okuda, Fumikazu Ohkawa, Shinichi Kato, Shoji Mitsufuji, Toshikazu Yoshikawa, Koji Takeuchi

    LIFE SCIENCES   83 ( 25-26 )   886 - 892   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Aims: We investigate the role of nitric oxide (NO) in the hypersecretion of acid and pepsinogen induced by stomach distension.
    Main method: The rat stomach was distended by instillation of saline through an acute fistula under urethane anesthesia.
    Key findings: Both secretions of acid and pepsinogen were increased by the distension depending on the volume of saline introduced, and responses were attenuated by bilateral cervical vagotomy or prior administration of atropine. N(G)-nitro-L-arginine methyl ester (L-NAME) had a dual effect on these responses, causing an increase in the acid response and a decrease in the pepsin response, both in an L-arginine-sensitive manner. Distension of the stomach increased the luminal NO release; this response was suppressed by vagotomy and L-NAME. Intragastric application of FK409, a NO donor, dose-dependently increased pepsinogen secretion while decreasing acid secretion in the stomach without distension. However, serosal application of both FK409 and 8-bromo-guanosine cyclic 3', 5'-monophosphate (8-Br-cGMP) stimulated the secretion of pepsinogen in isolated mouse stomachs in vitro. The stimulatory effect of FK409 on pepsinogen secretion was totally abolished by LY83583, a guanylate cyclase inhibitor.
    Significance: Distension of the stomach increases both acid and pepsinogen secretion through a vagalcholinergic pathway in addition to the luminal release of NO, and NO affects these responses in opposite ways, suppressing the acid response while enhancing the pepsin response, both mediated by a guanylate cyclase/cGMP pathway. (C) 2008 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.lfs.2008.10.010

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  • Increased susceptibility of small intestine to NSAID-provoked ulceration in rats with adjuvant-induced arthritis: Involvement of enhanced expression of TLR4 査読

    Shinichi Kato, Yasuyuki Ito, Hikaru Nishio, Yoko Aoi, Kikuko Amagase, Koji Takeuchi

    LIFE SCIENCES   81 ( 16 )   1309 - 1316   2007年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    NSAIDs damage the small intestine as well as the stomach as adverse effects. We previously reported that the gastric ulcerogenic response to NSAIDs was markedly increased in arthritic rats. The present study was designed to examine the intestinal ulcerogenic property of indomethacin in adjuvant-induced arthritic rats in comparison with normal animals. Arthritis was induced in male Dark Agouti rats by injection of Freund's complete adjuvant into the right hindfoot. Two weeks later, indomethacin was given orally and the intestine was examined for lesions at several time points after indomethacin. Indomethacin produced intestinal lesions in both normal and arthritic rats, but in the latter, the ulcerogenic response occurred much earlier and the severity was markedly enhanced. Amino, guanidine, an inhibitor of iNOS, significantly suppressed the damage, yet the efficacy differed in normal and arthritic rats, depending on the dose schedule; the effect of post-administration (6 h after) was greater than that of pre-administration (0.5 h before) in normal rats, whereas that of post-administration was less than that of pre-administration in arthritic rats. The expression of iNOS and TLR4 in the intestine was enhanced in arthritic rats as compared with normal rats. These results suggest that the intestinal ulcerogenic response to indomethacin is markedly aggravated in arthritic rats. Notably, the onset of the ulceration was much earlier in arthritic rats than normal rats. These phenomena may be accounted for by the upregulation of iNOS/NO through the increased expression of TLR4 in the small intestine of arthritic rats. (c) 2007 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.lfs.2007.08.036

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MISC

  • 定量リン酸化プロテオーム解析が明らかにした末梢神経再生時のGAP-43の新規リン酸化部位

    岡田 正康, 河嵜 麻実, 伊藤 泰行, 吉田 豊, 藤井 幸彦, 五十嵐 道弘

    JSBMS Letters   42 ( Suppl. )   88 - 88   2017年8月

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    記述言語:日本語   出版者・発行元:(一社)日本医用マススペクトル学会  

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  • 神経極性形成における4回膜貫通タンパク質-Glycoprotein M6a-のin vivo機能解析

    伊藤泰行, 本多敦子, 本多敦子, 武内恒成, 松下夏樹, 五十嵐道弘, 五十嵐道弘

    日本分子生物学会年会プログラム・要旨集(Web)   39th   2016年

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  • Electron Crystallography of Euglenoid Four-Transmembrane Protein Revealed the Linear Polymerization by a Combination of Three-Ways of Intermolecular Interaction

    Hiroshi Suzuki, Yasuyuki Ito, Kazutoshi Tani, Yuji Yamazaki, Masami Uji, Katsuhiko Mineta, Sachiko Tsukita, Yoshinori Fujiyoshi

    BIOPHYSICAL JOURNAL   104 ( 2 )   42A - 42A   2013年1月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:CELL PRESS  

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  • Pathogenic Importance of Endothelial Isozyme of Nitric Oxide Synthase (eNOS) in Aggravation of Gastric Lesions Induced By Indomethacin and Cold-Restraint Stress in Adjuvant-Induced Arthritic Rats

    Shinichi Kato, Yasuyuki Ito, Fumikazu Ohkawa, Koji Takeuchi

    GASTROENTEROLOGY   136 ( 5 )   A691 - A691   2009年5月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:W B SAUNDERS CO-ELSEVIER INC  

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  • Phosphodiesterase isozymes involved in regulatory mechanism of acid secretion in isolated mouse stomachs

    Yasuyuki Ito, Eitaro Aihara, Sayaka Okuda, Shinichi Kato, Koji Takeuchi

    GASTROENTEROLOGY   134 ( 4 )   A125 - A125   2008年4月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:W B SAUNDERS CO-ELSEVIER INC  

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  • Detrimental role of endothelial nitric oxide synthase (eNOS/NNOS3) in aggravation of indomethacin-induced gastric damage in adjuvant arthritis rats

    Shinichi Kato, Yasuyuki Ito, Fumikazu Ohkawa, Kikuko Amagase, Koji Takeuchi

    GASTROENTEROLOGY   134 ( 4 )   A220 - A220   2008年4月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:W B SAUNDERS CO-ELSEVIER INC  

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共同研究・競争的資金等の研究

  • 脳発生過程で最も高頻度にチロシンリン酸化されるMAP1Bの機能解明

    2021年9月 - 2024年4月

    武田科学振興財団  医学系研究奨励 

    伊藤 泰行

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    担当区分:研究代表者 

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  • 神経発生過程で最も高頻度にチロシンリン酸化される微小管結合タンパク質 MAP1B の役割

    2020年7月 - 2021年6月

    新潟大学塚田医学奨学基金  塚田医学奨学会助成金 

    伊藤泰行

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    担当区分:研究代表者 

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  • 高頻度チロシンリン酸化MAP1Bによる神経成長円錐のアクチン骨格制御機構の解明

    研究課題/領域番号:20K15897  2020年4月 - 2023年3月

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    伊藤 泰行

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    配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

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  • 高頻度チロシンリン酸化MAP1Bが担う神経成長円錐のアクチン骨格制御機構の解明

    2019年11月 - 2020年11月

    住友財団  基礎科学研究助成 

    伊藤 泰行

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    担当区分:研究代表者  資金種別:競争的資金

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  • 脳発生過程におけるMAP1Bの高頻度チロシンリン酸化部位の機能解析

    2019年4月 - 2021年9月

    藤井節郎記念 大阪基礎医学研究奨励会  藤井節郎記念大阪基礎医学研究奨励会助成金 

    伊藤 泰行

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    担当区分:研究代表者  資金種別:競争的資金

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  • 高頻度チロシンリン酸化MAP1Bが担う神経成長円錐のアクチン骨格制御機構の解明(生体内機能解析)

    2019年4月 - 2020年3月

    中島記念国際交流財団  日本人若手研究者研究助成金 

    伊藤 泰行

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    担当区分:研究代表者  資金種別:競争的資金

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  • 高頻度チロシンリン酸化MAP1B の神経発生における役割

    2018年1月 - 2019年4月

    上原記念生命科学財団  研究奨励金 

    伊藤 泰行

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    担当区分:研究代表者  資金種別:競争的資金

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  • 脳発生過程に関与するチロシンリン酸化修飾の網羅的解析と意義解明

    2016年9月 - 2019年5月

    武田科学振興財団  医学系研究奨励 

    伊藤 泰行

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    担当区分:研究代表者  資金種別:競争的資金

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  • Claudin類似4回膜貫通タンパク質Euglena-IP39の構造および機能解析

    2011年4月 - 2013年3月

    日本学術振興会  特別研究員奨励費 

    伊藤 泰行

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    担当区分:研究代表者  資金種別:競争的資金

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