researchmap

Language：English   Publisher：ELSEVIER SCIENCE BV  

Anthers of the Lilium x 'Enchantment', excised at the uninucleate microspore stage, were cultured on MS media containing 6% sucrose with auxin and cytokinin. When anthers were cultured on the medium with 2 mg l(-1) picloram and zeatin, 12-86% of them formed nodular calli. Anthers excised from greenhouse- and field-grown plants showed different responses: anthers of greenhouse-grown plants had a significantly higher capability to form callus and regenerate bulblets than those of field-grown plants. In anthers from greenhouse-grown plants, bulblet formation was dependent on the time at which anthers were excised from donor plants: anthers collected from early forced mother-plants had a higher capability of forming bulblet than others. All regenerated plantlets were diploid, which was substantiated by histological observation showing that the anther-derived calli originated from anther wall tissues. Virus tests by ELISA were made for 49 plantlets selected randomly at transplanting: 20 plantlets. (41%) were virus-free, and the rest showed positive reactions for lily symptomless virus, cucumber mosaic virus and/or tulip breaking virus. (C) 2001 Elsevier Science B.V. All rights reserved.

DOI： 10.1016/S0304-4238(01)00223-0

Web of Science

researchmap

Language：English   Publisher：ELSEVIER SCIENCE BV  

Anthers of the Lilium x 'Enchantment', excised at the uninucleate microspore stage, were cultured on MS media containing 6% sucrose with auxin and cytokinin. When anthers were cultured on the medium with 2 mg l(-1) picloram and zeatin, 12-86% of them formed nodular calli. Anthers excised from greenhouse- and field-grown plants showed different responses: anthers of greenhouse-grown plants had a significantly higher capability to form callus and regenerate bulblets than those of field-grown plants. In anthers from greenhouse-grown plants, bulblet formation was dependent on the time at which anthers were excised from donor plants: anthers collected from early forced mother-plants had a higher capability of forming bulblet than others. All regenerated plantlets were diploid, which was substantiated by histological observation showing that the anther-derived calli originated from anther wall tissues. Virus tests by ELISA were made for 49 plantlets selected randomly at transplanting: 20 plantlets. (41%) were virus-free, and the rest showed positive reactions for lily symptomless virus, cucumber mosaic virus and/or tulip breaking virus. (C) 2001 Elsevier Science B.V. All rights reserved.

DOI： 10.1016/S0304-4238(01)00223-0

Web of Science

researchmap

Language：English   Publisher：KLUWER ACADEMIC PUBL  

A haploid callus line from anther cultures of the Asiatic hybrid lily 'Connecticut King' was maintained for a long term. The survival and growth of the haploid calluses were affected by auxins of picloram, alpha-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) and temperatures of 25, 15 and 7 degrees C during culture. Picloram was more suitable for maintenance of the haploid calluses, whereas NAA and 2,4-D led to root and shoot formation from the haploid calluses. The best temperature for maintenance was 25 degrees C. About 90% of cells in calluses were maintained in haploid level during 60 weeks of subculture, and about 80% of cells were haploid in the calluses maintained over 2 years with the MS medium containing 4 mu M picloram in the dark at 25 degrees C.

DOI： 10.1023/A:1006425307530

Web of Science

CiNii Article

researchmap

researchmap

researchmap

Language：English  

A haploid callus line from anther cultures of the Asiatic hybrid lily 'Connecticut King' was maintained for a long term. The survival and growth of the haploid calluses were affected by auxins of picloram, α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) and temperatures of 25, 15 and 7 °C during culture. Picloram was more suitable for maintenance of the haploid calluses, whereas NAA and 2,4-D led to root and shoot formation from the haploid calluses. The best temperature for maintenance was 25 °C. About 90% of cells in calluses were maintained in haploid level during 60 weeks of subculture, and about 80% of cells were haploid in the calluses maintained over 2 years with the MS medium containing 4 μM picloram in the dark at 25°C.

DOI： 10.1023/A:1006425307530

Scopus

CiNii Article

researchmap

DOI： 10.2503/jjshs.69.52

researchmap

DOI： 10.2503/jjshs.68.979

researchmap

DOI： 10.2503/jjshs.68.979

researchmap

Language：English   Publisher：Kluwer Academic Publishers  

A system for producing haploid plants from anther cultures was developed for the Asiatic hybrid lily 'Connecticut King'. Anthers containing microspores at the mid- to late-uninucleate stages were cultured on MS media supplemented with various plant growth regulators. Microspores containing 3 or 4 vegetative-like nuclei were observed 2 to 3 weeks later, and yellowish nodular calluses appeared within dehisced anthers 2 to 3 months after culture. Picloram was Superior to 2,4-D for inducing nodular calluses. Anthers from greenhouse-grown plants required higher concentrations of both picloram and cytokinins than those from field-grown plants and most frequently produced nodular calluses (17.6%) on MS medium containing 2 mg l-1 picloram and 2 mg l-1 zeatin. The nodular calluses regenerated many bulblets following transfer to MS medium supplemented with 0.1 or 0.5 mg l-1 picloram and 0.01 mg l-1 BA, and the bulblets developed into plantlets (bulblets with scaly leaves and roots) after transfer to MS medium containing 0.1 mg l-1 NAA. Chromosome counts of root-tip cells of 11 plantlets revealed that five were haploids (2n = 12), two diploids (2n = 24), and four mixoploid. This result suggests that at least some plantlets were of gametophytic origin.

DOI： 10.1007/BF02318951

Scopus

CiNii Article

researchmap

DOI： 10.1007/BF02318951

researchmap

researchmap

researchmap

researchmap

researchmap

researchmap

researchmap

researchmap