Updated on 2022/12/01

写真a

 
IWASAKI Toshisuke
 
Organization
Academic Assembly Institute of Science and Technology CHIKYU SEIBUTSU KAGAKU KEIRETU Associate Professor
Graduate School of Science and Technology Life and Food Sciences Associate Professor
Faculty of Science Department of Science Associate Professor
Title
Associate Professor
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Degree

  • 理学博士 ( 1991.3   大阪大学 )

Research Interests

  • イネ

  • 光応答遺伝子発現

  • インポ-ティンα

  • タンパク質核輸送

  • インポーティンα結合タンパク質

  • タンパク質間相互作用

  • 蛋白質核移行

  • 光環境応答

  • 蛋白質間相互作用

  • TPR配列

  • 核局在化シグナル

  • 核タンパク質

  • T-DNAタグライン

  • インボーティンα結合タンパク質

  • アフィニティークロマトグラフィー

  • 核移行シグナル受容体

  • シロイヌナズナ

Research Areas

  • Life Science / Plant molecular biology and physiology

Research History (researchmap)

  • Niigata University   Faculty of Science   Associate Professor (as old post name)

    1998.1 - 2004.3

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  • Niigata University   Faculty of Science   Research Assistant

    1996.6 - 1997.12

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  • National Institute of Agrobiological Research   COE postdoctoral fellow

    1994.10 - 1996.5

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  • Plant Molecular Biology Laboratory, RIKEN   Special Postdoctoral Researcher

    1992.4 - 1994.9

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  • Osaka University

    1988.4 - 1990.3

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Research History

  • Niigata University   Faculty of Science Department of Science   Associate Professor

    2017.4

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2004.4

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2004.4

  • Niigata University   Faculty of Science Department of Biology   Associate Professor

    2004.4 - 2017.3

  • Niigata University   Associate Professor (as old post name)

    1998.1 - 2004.3

  • Niigata University   Faculty of Science   Research Assistant

    1996.6 - 1997.12

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Education

  • Osaka University   School of Science   生物学科 研究生

    1990.4 - 1992.3

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  • 大阪大学大学院   理学研究科   生理学専攻 博士後期課程

    1985.4 - 1990.3

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  • 大阪大学大学院   理学研究科   生理学専攻 博士前期課程

    1983.4 - 1985.3

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  • Nagoya University   School of Science   生物学科

    1981.4 - 1983.3

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  • Nagoya University   教養部   理学部進学課程

    1979.4 - 1981.3

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  • 高槻高等学校

    1976.4 - 1979.3

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  • 大阪大学大学院   理学研究科   生理学専攻 博士後期課程 修了(理学博士)

    1991.3

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Committee Memberships

  • 独立行政法人 大学入試センター   教科科目第一委員会委員  

    2009.4 - 2011.3   

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    Committee type:Other

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Papers

  • Arabidopsis VIP6/ELF8, the homolog of CTR9 component of the transcriptional complex PAF1, is essential for plant development Reviewed

    Takeshi Shiraya, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Toshisuke Iwasaki

    PLANT BIOTECHNOLOGY   25 ( 5 )   447 - 455   2008

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY  

    The VERNALIZATION INDEPENDENCE 6 (VIP6)/EARLY FLOWERING 8 (ELF8) gene of Arabidopsis thaliana encodes a protein homologous to CYCLIN THREE REQUIRING 9 (CTR9) component of the yeast transcriptional complex, RNA polymerase II-associated factor 1 (PAF1). It has been demonstrated that mutation alleles in the VIP6/ELF8 show early flowering phenotype, as well as other pleiotropic developmental defects. Here, we provide evidence that seeds with vip6/elf8 homozygous mutations were rarely obtained in all three independent lines of T-DNA insertion. Although viable seeds with homozygous mutant alleles were rarely obtained, they showed pleiotropic phenotype like mutants of other PAF1-related genes, and were sterile. These results suggest that the VIP6/ELF8 gene is essential for plant development.

    DOI: 10.5511/plantbiotechnology.25.447

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  • Functional analysis and intracellular localization of rice cryptochromes Reviewed

    N Matsumoto, T Hirano, T Iwasaki, N Yamamoto

    PLANT PHYSIOLOGY   133 ( 4 )   1494 - 1503   2003.12

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    Blue-light-receptor cryptochrome (CRY), which mediates cotyledon expansion, increased accumulation of anthocyanin, and inhibition of hypocotyl elongation, was first identified in Arabidopsis.. Two Arabidopsis cryptochromes (AtCRY1 and AtCRY2) have been reported to be localized to the nucleus. However, there is no information on the cryptochromes in monocotyledons. In this study, we isolated two cryptochrome cDNAs, OsCRY1 and OsCRY2, from rice (Oryza sativa) plants. The deduced amino acid sequences of OsCRY1 and CsCRY2 have a photolyase-like domain in their N termini and are homologous to AtCRY1. To investigate the function of OsCRY1, we overexpressed a green fluorescence protein-OsCRY1 fusion gene in Arabidopsis and assessed the phenotypes of the resulting transgenic plants. When the seedlings were germinated in the dark, no discernible effect was observed. However, light-germinated seedlings showed pronounced inhibition of hypocotyl elongation and increased accumulation of anthocyanin. These phenotypes were induced in a blue-light-dependent manner, indicating that OsCRY1 functions as a blue-light-receptor cryptochrome. We also examined the intracellular localization of green fluorescence protein-OsCRY1 in the transgenic plants. It was localized to both the nucleus and the cytoplasm. We identified two nuclear localization domains in the primary structure of OsCRY1. We discuss the relationship between the function and intracellular localization of rice cryptochromes by using additional data obtained with OsCRY2.

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  • Molecular cloning of a novel importin alpha homologue from rice, by which constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-protein is preferentially nuclear imported Reviewed

    CJ Jiang, K Shoji, R Matsuki, A Baba, N Inagaki, H Ban, T Iwasaki, N Imamoto, Y Yoneda, XW Deng, N Yamamoto

    JOURNAL OF BIOLOGICAL CHEMISTRY   276 ( 12 )   9322 - 9329   2001.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Nuclear import of proteins that contain classical nuclear localization signals (NLS) is initiated by importin alpha, a protein that recognizes and binds to the NLS in the cytoplasm. In this paper, we have cloned a cDNA for a novel importin ct homologue from rice which is in addition to our previously isolated rice importin alpha 1a and alpha2, and we have named it rice importin alpha 1b. In vitro binding and nuclear import assays using recombinant importin alpha 1b protein demonstrate that rice importin alpha 1b functions as a component of the NLS-receptor in plant cells. Analysis of the transcript levels for till three rice importin alpha genes revealed that the genes were not only differentially expressed but that they also responded to dark adaptation in green leaves. Furthermore, we also show that the COP1 protein bears a bipartite-type NLS and its nuclear import is mediated preferentially by the rice importin alb. These data suggest that each of the different; rice importin alpha proteins carry distinct groups of nuclear proteins, such that multiple isoforms of importin alpha contribute to the regulation of plant nuclear protein transport.

    DOI: 10.1074/jbc.M006430200

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  • Purification, characterization and cDNA cloning of soluble carbonic anhydrase from Chlorella sorokiniana grown under ordinary air Reviewed

    Akira Satoh, Toshisuke Iwasaki, Shoji Odani, Yoshihiro Shiraiwa

    Planta   206 ( 4 )   657 - 665   1998.11

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    Soluble carbonic anhydrase (CA, EC 4.2.1.1) inducible by low levels of CO2 was purified from the unicellular green alga Chlorella sorokiniana grown at alkaline pH. The purified CA had a specific activity of 2,300 units (mg protein)-1. The molecular mass of the CA was found to be 100 kDa by non-dissociating (native)-polyacrylamide gel electrophoresis and 50 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 50-kDa subunit was recognized by concanavalin A. These results suggest that the protein has a dimeric form with two 50-kDa subunits that are glycosylated in an asparagine-linked manner. The native CA was revealed by isoelectric focusing to be a very acidic protein with an isoelectric point of 4.2. About 60% of the CA activity was inhibited by 0.5 M NaCl. The enzyme was inactivated over 95% by preincubation with 50 mM dithiothreitol but not with l mM dithiothreitol. After partial amino acid sequence analysis, a cDNA clone of the CA was isolated and characterized. The cloned cDNA fragment encoded a 348-amino-acid polypeptide (36,709 Da) including an NH2-terminal hydrophobic signal peptide composed of 35 amino acids (3,725 Da). Conserved regions of sequences found in animal CAs, in the periplasmic (pCA) and the intracellular CAs of Chlamydomonas, and in the plasmamembrane-bound CA of Dunaliella (Dca) were also found in this Chlorella CA. The signal sequence was significantly homologous to the pCA and the Dca. The internal signal sequence between the large and the small subunits reported for pCA was not found in this Chlorella CA. The soluble CA of this alga was an αtype CA with salt-sensitive, periplasm-locating and acidic properties and very different from pCA and Dca with their salt-sensitive/neutral and salt-resistant/acidic properties, respectively.

    DOI: 10.1007/s004250050444

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  • Isolation and characterization of two importin-beta genes from rice Reviewed

    R Matsuki, T Iwasaki, K Shoji, CJ Jiang, N Yamamoto

    PLANT AND CELL PHYSIOLOGY   39 ( 8 )   879 - 884   1998.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOC PLANT PHYSIOLOGISTS  

    The nuclear transport of proteins is mediated by the complex of importin-alpha and importin-beta. We isolated two cDNAs encoding importin-beta from rice. A rice importin-beta was demonstrated to interact with rice GST-importin-alpha fusion proteins. The presence of two importin-beta genes was shown for the first time among a variety of eukaryotes.

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  • Evolutionary origin of two genes for chloroplast small heat shock protein of tobacco Reviewed

    BH Lee, Y Tanaka, T Iwasaki, N Yamamoto, T Kayano, M Miyao

    PLANT MOLECULAR BIOLOGY   37 ( 6 )   1035 - 1043   1998.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:KLUWER ACADEMIC PUBL  

    Two different cDNA clones for the chloroplast small heat shock protein (smHSP) were isolated from tobacco (Nicotiana tabacum cv. Petit Havana SR1). One of the cDNAs (type I) has a full-length open reading frame (ORF) of the smHSP of 26.6 kDa. By contrast, the other one (type II) contains an additional nucleotide that causes the frame shift inside a putative ORF for the smHSP. If this nucleotide is neglected, type II cDNA encodes the smHSP that is 89% identical to that encoded by type I cDNA. Southern blot and polymerase chain reaction (PCR) analyses with genomic DNA indicated that tobacco has two different smHSP genes while two ancestors of tobacco, N. sylvestris and N. tomentosiformis, have a single gene that each corresponds to one of the two genes of tobacco. It was also found that one of the tobacco genes has an ORF for the smHSP disrupted by nucleotide insertion in the same way as type II cDNA, while both ancestor genes have a functional ORE These results suggest that the two smHSP genes of tobacco had been derived from the two ancestor species, and that one of the two genes had been disrupted by nucleotide insertion during the course of the evolution of tobacco. Northern blot and reverse transcription (RT)-PCR analyses demonstrated that both the tobacco genes are expressed upon heat stress, exhibiting different dependences on temperature.

    DOI: 10.1023/A:1006067817058

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  • Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves Reviewed

    K Shoji, T Iwasaki, R Matsuki, M Miyao, N Yamamoto

    GENE   212 ( 2 )   279 - 286   1998.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    The import of nuclear proteins into nuclei begins with recognition of nuclear localization signal-harboring proteins and binding to a nuclear pore targeting complex. A cDNA for an importin-alpha protein, a subunit of the complex, was isolated from rice plants. The amino acid sequence deduced from the nucleotide sequence of the cDNA exhibited a high homology to those of importin-alpha proteins from many organisms such as Arabidopsis thaliana, Saccharomyces cerevisiae, human, mouse, Xenopus laevis and Drosophila melanogaster. Down-regulation of the transcription by light was shown in the leaves of light- and dark-grown seedlings by RNA blot analysis. The down-regulation was specific to leaves, whereas no light effect was observed in root tissues or calli, in which higher levels of the transcript were detected. (C) 1998 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-1119(98)00175-9

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  • A novel importin alpha from rice, a component involved in the process of nuclear protein transport Reviewed

    T Iwasaki, R Matsuki, K Shoji, K Sanmiya, M Miyao, N Yamamoto

    FEBS LETTERS   428 ( 3 )   259 - 262   1998.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    In cukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin alpha. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin alpha. Although the encoded protein had only 23-27% amino acid identity to the importin alpha s from various organisms including plants, the fusion protein,vith glutathione S-transferase showed a specific binding activity to the NLS of SV40 T-antigen, These results suggest that the rice #61L protein is a novel importin alpha in plants. (C) 1998 Federation of European Biochemical Societies.

    DOI: 10.1016/S0014-5793(98)00540-7

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  • The dehydration-inducible rd17 (cor47) gene and its promoter region in Arabidopsis thaliana (Accession No. AB004872). (PGR97-156) Reviewed

    T. Iwasaki, T. Kiyosue, K. Yamaguchi-Shinozaki, K. Shinozaki

    Plant Physiol.   115 ( 3 )   1287 - 1287   1997.11

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  • Role of Arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression Reviewed

    H Abe, K YamaguchiShinozaki, T Urao, T Iwasaki, D Hosokawa, K Shinozaki

    PLANT CELL   9 ( 10 )   1859 - 1868   1997.10

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    In Arabidopsis, the induction of a dehydration-responsive gene, rd22, is mediated by abscisic acid (ABA) and requires protein biosynthesis for ABA-dependent gene expression. Previous experiments established that a 67-bp DNA fragment of the rd22 promoter is sufficient for dehydration- and ABA-induced gene expression and that this DNA fragment contains two closely located putative recognition sites for the basic helix-loop-helix protein MYC and one putative recognition site for MYB. We have carefully analyzed the 67-bp region of the rd22 promoter in transgenic tobacco plants and found that both the first MYC site and the MYB recognition site function as cis-acting elements in the dehydration-induced expression of the rd22 gene. A cDNA encoding a MYC-related DNA binding protein was isolated by DNA-ligand binding screening, using the 67-bp region as a probe, and designated rd22BP1. The rd22BP1 cDNA encodes a 68-kD protein that has a typical DNA binding domain of a basic region helix-loop-helix leucine zipper motif in MYC-related transcription factors. The rd22BP1 protein binds specifically to the first MYC recognition site in the 67-bp fragment. RNA gel blot analysis revealed that transcription of the rd22BP1 gene is induced by dehydration stress and ABA treatment, and its induction precedes that of rd22. We have reported a drought-and ABA-inducible gene that encodes the MYB-related protein ATMYB2. In a transient transactivation experiment using Arabidopsis leaf protoplasts, we demonstrated that both the rd22BP1 and ATMYB2 proteins activate transcription of the rd22 promoter fused to the p-glucuronidase reporter gene. These results indicate that both the rd22BP1 (MYC) and ATMYB2 (MYB) proteins function as transcriptional activators in the dehydration- and ABA-inducible expression of the rd22 gene.

    DOI: 10.1105/tpc.9.10.1859

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  • Cloning of a cDNA that encodes farnesyl diphosphate synthase and the blue-light-induced expression of the corresponding gene in the leaves of rice plants Reviewed

    K Sanmiya, T Iwasaki, M Matsuoka, M Miyao, N Yamamoto

    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION   1350 ( 3 )   240 - 246   1997.2

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    A cDNA encoding farnesyl diphosphate synthase (FPPS), a key enzyme in isoprenoid biosynthesis, was isolated from a cDNA library constructed from mRNA that had been prepared from etiolated rice (Oriza sativa L. variety Nipponbare) seedlings after three hours of illumination by a subtraction method. The putative polypeptide deduced from the 1289 bp nucleotide sequence consisted of 353 amino acids and had a molecular mass of 40676 Da. The predicted amino acid sequence exhibited high homology to those of FPPS from Arabidopsis (73% to type 1, 72% to type 2) and white lupin (74%). Southern blot analysis showed that the rice genome might contain only one gene for FPPS. The highest level of expression of the gene was demonstrated in leaves by RNA blot analysis. Moreover, light, in particular blue light, effectively enhanced expression of the gene.

    DOI: 10.1016/S0167-4781(96)00231-X

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  • Cloning of cDNA encoding the rice 22 kDa protein of Photosystem II (PSII-S) and analysis of light-induced expression of the gene Reviewed

    T Iwasaki, Y Saito, E Harada, M Kasai, K Shoji, M Miyao, N Yamamoto

    GENE   185 ( 2 )   223 - 229   1997.2

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    Cloning of rice cDNA encoding the chlorophyll-binding 22 kDa protein of Photosystem II (PSII-S) and the light-induced expression of the gene are reported. One of the light-responsive cDNA clones, isolated by screening with a light-specific subtracted cDNA probe, was shown to encode PSII-S of rice. Genomic Southern analysis suggested that the PSII-S gene, psbS, is a single-copy gene in rice. A brief exposure to red light induced a severalfold increase in the steady state level of PSII-S transcripts in etiolated seedlings. The red light effect was reversed by far-red light, suggesting involvement of phytochrome in the PSII-S gene regulation. Prolonged exposure (3 h) to blue light, however, revealed a much stronger effect than red light on the accumulation of PSII-S transcripts in the etiolated seedlings. In dark-adapted green plants, prolonged exposure to blue light induced re-accumulation of transcripts encoding PSII-S, whereas red light had little effect.

    DOI: 10.1016/S0378-1119(96)00646-4

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  • IDENTIFICATION OF A CIS-REGULATORY REGION OF A GENE IN ARABIDOPSIS-THALIANA WHOSE INDUCTION BY DEHYDRATION IS MEDIATED BY ABSCISIC-ACID AND REQUIRES PROTEIN-SYNTHESIS Reviewed

    T IWASAKI, K YAMAGUCHISHINOZAKI, K SHINOZAKI

    MOLECULAR AND GENERAL GENETICS   247 ( 4 )   391 - 398   1995.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    In Arabidopsis thaliana, the induction of a dehydration-responsive gene, rd22, is mediated by abscisic acid (ABA) but the gene does not include any sequence corresponding to the consensus ABA-responsive element (ABRE), RYACGTGGYR, in its promoter region. The cis-regulatory region of the rd22 promoter was identified by monitoring the expression of beta-glucuronidase (GUS) activity in leaves of transgenic tobacco plants transformed with chimeric gene fusions constructed between 5'-deleted promoters of rd22 and the coding region of the GUS reporter gene. A 67-bp nucleotide fragment corresponding to positions - 207 to - 141 of the rd22 promoter conferred responsiveness to dehydration and ABA on a non-responsive promoter. The 67-bp fragment contains the sequences of the recognition: sites for some transcription factors, such as MYC, MYB, and GT-1. The fact that accumulation of rd22 mRNA requires protein synthesis raises the possibility that the expression of rd22 might be regulated by one of these trans-acting protein factors whose de novo synthesis is induced by dehydration or ABA. Although the structure of the RD22 protein is very similar to that of a non-storage seed protein, USP, of Vicia faba, the expression of the GUS gene driven by the rd22 promoter in non-stressed transgenic Arabidopsis plants was found mainly in flowers and bolted stems rather than in seeds.

    DOI: 10.1007/BF00293139

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  • Function and regulation of genes that are induced by drought stress in Arabidopsis thaliana Reviewed

    K. Yamaguchi-Shinozaki, T. Urao, T. Iwasaki, T. Kiyosue, K. Shinozaki

    JIRCAS Journal   1   69 - 79   1994.11

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  • A drought-inducible myb-related gene in plants Reviewed

    T. Urao, T. Iwasaki, K. Yamaguchi-Shinozaki, K. Shinozaki

    RIKEN Review   6   23 - 24   1994.7

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  • CHARACTERIZATION OF 2 CDNAS THAT ENCODE MAP KINASE HOMOLOGS IN ARABIDOPSIS-THALIANA AND ANALYSIS OF THE POSSIBLE ROLE OF AUXIN IN ACTIVATING SUCH KINASE-ACTIVITIES IN CULTURED-CELLS Reviewed

    T MIZOGUCHI, Y GOTOH, E NISHIDA, K YAMAGUCHISHINOZAKI, N HAYASHIDA, T IWASAKI, H KAMADA, K SHINOZAKI

    PLANT JOURNAL   5 ( 1 )   111 - 122   1994.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BLACKWELL SCIENCE LTD  

    Two cDNA clones, cATMPK1 and cATMPK2, encoding MAP kinases (mitogen-activated protein kinases) have been cloned from Arabidopsis thaliana and their nucleotide sequences have been determined. Putative proteins encoded by ATMPK1 and ATMPK2 genes, designated ATMPK1 and ATMPK2, contain 370 and 376 amino acid residues, respectively, and are 88.7% identical at the amino acid sequence level. ATMPK1 and ATMPK2 exhibit significant similarity to rat ERK2 (49%) and Xenopus MAP kinase (50%). The amino acid residues corresponding to the sites of phosphorylation (Thr-Glu-Tyr) that are involved in the activation of MAP kinases are conserved in ATMPK1 and ATMPK2. Northern blot analysis indicates that the ATMPK1 and ATMPK2 mRNAs are significantly present in all the organs except seeds. Genomic Southern blot analysis suggests that there are a few additional genes that are related to ATMPK1 and ATMPK2 in the Arabidopsis genome. Purified Xenopus MAP kinase kinase (MAPK kinase) phosphorylates ATMPK1 and ATMPK2 proteins that have been expressed in Escherichia coli, activating these enzymes. A rapid and transient activation of 46-kDa protein kinase activity that phosphorylated myelin basic protein (MBP) was detected when auxinstarved tobacco BY-2 cells were treated with synthetic auxin, 2,4-dichlorophenoxyacetic acid (2,4-D). Protein kinase activities which phosphorylated the recombinant ATMPK2 protein also increased rapidly after auxin treatment in the auxin-starved BY-2 cells. These results suggest that auxin may function as an activator of plant MAP kinase homologues, as do various mitogens in animal systems.

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  • BRASSINOSTEROIDS ACT AS REGULATORS OF TRACHEARY-ELEMENT DIFFERENTIATION IN ISOLATED ZINNIA MESOPHYLL-CELLS Reviewed

    T IWASAKI, H SHIBAOKA

    PLANT AND CELL PHYSIOLOGY   32 ( 7 )   1007 - 1014   1991.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOC PLANT PHYSIOLOGISTS  

    Uniconazole [S-3307; (E)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol], a synthetic plant-growth retardant, inhibited the differentiation of isolated mesophyll cells of Zinnia elegans L. into tracheary elements (TEs) but had no effect on cell division when it was added to the culture medium at a concentration of 3.4-mu-M. In the presence of uniconazole, none of the cytological events characteristic of the processes of TE differentiation, such as aggregation of actin filaments, bundling of microtubules or localized thickening and lignification of secondary walls, was observed. Uniconazole was effective when it was added to the medium within 36 h after the start of culture. Brassinosteroids (0.2 nM brassinolide or 2-mu-M homobrassinolide), but not gibberellin A3, counteracted the inhibitory effect of uniconazole on TE differentiation. Brassinosteroids were most effective when they were added to cultures between 24 and 30 h after the start of culture. Exogenously applied brassinosteroids promoted TE differentiation. It is suggested that the synthesis of brassinosteroids is essential for the differentiation of the cells into TEs and that uniconazole inhibits this differentiation through its inhibitory effect on the biosynthesis of brassinosteroids.

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  • RELATIONSHIP BETWEEN DNA-SYNTHESIS AND THE INCREASE IN THE LEVEL OF TUBULIN DURING DEDIFFERENTIATION OF ISOLATED ZINNIA MESOPHYLL-CELLS Reviewed

    T IWASAKI, H FUKUDA, H SHIBAOKA

    PROTOPLASMA   143 ( 2-3 )   130 - 138   1988

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  • INHIBITION OF CELL-DIVISION AND DNA-SYNTHESIS BY GIBBERELLIN IN ISOLATED ZINNIA MESOPHYLL-CELLS Reviewed

    T IWASAKI, H FUKUDA, H SHIBAOKA

    PLANT AND CELL PHYSIOLOGY   27 ( 4 )   717 - 724   1986.6

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MISC

  • 重力刺激によるイネ葉身傾斜誘導は葉身関節における局所的なスーパーオキシドラジカルの発生を伴う

    齋藤智史, 吉田翔馬, 桂野敦史, 武田勇生, 岩崎俊介

    日本植物学会第73回大会 研究発表記録   215   2009.9

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    イネ葉身傾斜は、ラミナジョイント(葉身関節)に限局的な偏差成長によって、葉鞘の軸に対して葉身が傾く成長運動であり、外生のブラシノステロイド(BR)やオーキシンによって促進されることはよく知られている。イネ葉身傾斜を誘導する環境要因としては、連続青色光や横向きの重力刺激が知られているが、これらの刺激がどのような過程を経て葉身傾斜をもたらすかは不明である。今回私たちは、イネ葉身傾斜誘導に活性酸素種が関与する可能性を検討し、重力刺激による葉身傾斜誘導にスーパーオキシドラジカル(O2-)発生が関与することを示唆する結果を得たので報告する。第三葉抽出前のイネ黄化芽ばえ全体、あるいはそれから切り取った第二葉葉片に対して、暗所で横向きの重力刺激を与えることによって葉身傾斜を誘導し、NBTによる組織化学染色を行ったところ、葉身傾斜に伴って葉身関節でO2-が発生することが確認された。葉身関節におけるO2-の発生部位は、時間経過とともに葉の外縁部から向軸側に広がっていくように見えた。また、O2-スカベンジャーであるTiron、O2-を発生させるNADPHオキシダーゼの阻害剤であるDPI、オーキシン輸送阻害剤であるNPAは、いずれも葉身関節におけるO2-発生とともに葉身傾斜を阻害した。以上の結果は、葉身関節におけるO2-の発生が葉身傾斜の誘導要因として働く可能性を示唆している。

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  • シロイヌナズナvip6/elf8は有性生殖に必須の遺伝子である

    白矢 武士, 佐藤 修正, 加藤 友彦, 田畑 哲之, 岩崎 俊介

    日本植物生理学会年会およびシンポジウム 講演要旨集   649   2008

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    DOI: 10.14841/jspp.2008.0.0649.0

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  • Light-responsive changes in intracellular localization of rice homolog of CTR9, a component of yeast PAF1 complex

    Takeshi Shiraya, Aya Kitajima, Maho Tozawa, Changhong Guo, Hiroshi Ban, Naoki Yamamoto, Toshiaki Mitsui, Toshisuke Iwasaki

    2007.12

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  • Light-responsive changes in intracellular localization of rice homolog of CTR9, a component of yeast PAF1 complex

    Takeshi Shiraya, Aya Kitajima, Maho Tozawa, Changhong Guo, Hiroshi Ban, Naoki Yamamoto, Toshiaki Mitsui, Toshisuke Iwasaki

    The 5th International Symposium of Rice Functional Genomics   PO-033   2007.10

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    We isolated cDNA for a novel protein IABP4 that binds specifically to a particular importin a protein whose expression is down-regulated by light in rice plants. IABP4 consists of a long N-terminal tetratricopeptide repeat (TPR) domain, the presence of which implies activity to form a protein complex, and a short C-terminal domain that contains three putative nuclear localization signals (NLSs). The deduced amino acid sequence of IABP4 was homologous to that of a yeast protein CTR9, which is known to function, as a subunit of the nuclear complex called PAF1, in transcriptional initiation and elongation, histone modification and control of cell cycle. Therefore, we hypothesized that rice IABP4 also functions by forming a nuclear complex, and that its nuclear localization is regulated by light. In this study, we investigated the intracellular localization of IABP4 and the effect of light on it. First, we examined the intracellular localization of green fluorescent protein (GFP) fusions with full length or various fragments of IABP4 in leaf epidermal cells of onion or Tradescantia virginiana. As a result, we identified one NLS and one NES as functional, respectively. However, the fusion protein with the full length IABP4 was localized both in the nucleus and the cytoplasm. These results suggested that intracellular localization of native IABP4 would change by the balance between nuclear import and export in response to the environmental conditions such as light. So, next we examined whether or not IABP4 will change its intracellular localization in response to light. When the epidermis was incubated under continuous light after particle-bombardment, the nuclear-to-cytoplasmic ratio for the intensity of the fluorescence was decreased, whereas it did not change when the epidermis was incubated in the dark. These results support the idea that IABP4 plays some important roles in light-response of plants throughits light-dependent shuttling between the nucleus and the cytoplasm.

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  • 花成関連タンパク質VIP6/ELF8は胚発生に必須であるか?

    白矢 武士, 佐藤 修正, 加藤 友彦, 田畑 哲之, 岩崎 俊介

    日本植物生理学会年会およびシンポジウム 講演要旨集   693   2007

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  • イネのTPR含有核タンパク質の核局在化シグナルの同定

    白矢 武士, 戸沢 真穂, 郭 長虹, 伴 浩志, 山本 直樹, 岩崎 俊介

    日本植物生理学会年会およびシンポジウム 講演要旨集   551   2007

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    DOI: 10.14841/jspp.2007.0.551.0

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  • Identification of the functional nuclear localization signal of a rice tetratricopeptide repeat-containing nuclear protein

    Takeshi Shiraya, Maho Tozawa, Changhong Gu, Hiroshi Ban, Naoki Yamamoto, Toshisuke Iwasaki

    PLANT AND CELL PHYSIOLOGY   48   S154 - S154   2007

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  • Is the flowering-associated protein VIP6/ELF8 essential for embryogenesis?

    Takeshi Shiraya, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Toshisuke Iwasaki

    PLANT AND CELL PHYSIOLOGY   48   S190 - S190   2007

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  • イネブラシノステロイド生合成遺伝子の発現に対する青色光の影響

    福田 泰規, 澤田 義昭, 岩崎 俊介

    日本植物生理学会年会およびシンポジウム 講演要旨集   668   2005

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    DOI: 10.14841/jspp.2005.0.668.0

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  • Brassinosteroids in blue light-induced lamina inclination in rice

    Iwasaki Toshisuke

    Japanese Journal of Crop Science   71 ( 1 )   306 - 307   2002.4

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  • importin α との相互作用によるイネ新奇核タンパク質の同定

    郭長虹, 伴浩志, 本間美智紀, 山本直樹, 岩崎俊介

    日本植物生理学会年会およびシンポジウム 講演要旨集   2002

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    importin αは,真核生物の細胞質においてタンパク質の核局在化シグナル(NLS)を認識してimportin βと結びつけ,核輸送複合体を形成する。イネのimportin α1aは,葉において負の光応答発現を示すことから,暗条件下で核に蓄積するタンパク質の核移行に関与する可能性が考えられる。そのような新規核タンパク質の単離・同定を目的として,α1aをプローブとするFar Western法によりイネの黄化葉cDNAライブラリーをスクリーニングした結果,新規な122 kDaタンパク質をコードするcDNAクローンが単離された。この新規遺伝子も負の光応答発現を示すこと、さらに推定NLSを含むC末端断片はin vitroでα1aに特異的に結合することが示された。

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  • Intracellular localization and functional analysis of rice cryptochromes

    N Matsumoto, T Iwasaki, N Yamamoto

    PLANT AND CELL PHYSIOLOGY   43   S230 - S230   2002

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  • MOLECULAR CLONING AND FUNCTIONAL ANALYSIS OF A NOVEL RICE IMPORTIN α HOMOLOGUE

    JIANG Chang-Jie, MATSUKI Rikyu, SHOJI Kazuhiro, BAN Hiroshi, IWASAKI Toshisuke, YAMAMOTO Naoki

    40   s67 - s67   1999.3

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  • cDNA CLONING AND CHARACTERIZATION OF LOW-CO_2-INDUCIBLE SOLUBLE CARBONIC ANHYDRASE IN Chlorella sorokiniana

    SATOH Akira, IWASAKI Toshisuke, SHIRAIWA Yoshihiro

    39   S27 - S27   1998.5

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  • CLONING OF A PROTEIN THAT INTERACTS WITH α SUBUNIT OF THE NUCLEAR PROTEIN TRANSPORT COMPLEX IN RICE

    BAN Hiroshi, MATSUKI Rikyu, IWASAKI Toshisuke, YAMAMOTO Naoki

    39   S130 - S130   1998.5

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  • イネ核輸送タンパク質Importin βのcDNAの単離と発現解析

    松木 吏弓, 岩崎 俊介, 庄子 和博, 山本 直樹

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   61   316 - 316   1997.9

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  • Light regulation of a gene encoding NLS receptor protein in rice.

    N Yamamoto, K Shoji, R Matsuki, T Iwasaki, H Uchimiya, M Miyao

    PLANT PHYSIOLOGY   114 ( 3 )   1279 - 1279   1997.7

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  • ANALYSES OF THE rd22BP1 GENE ENCODING MYC-RELATED PROTEIN THAT SPECIFICALY BINDS TO A CIS-ACTING ELEMENT INVOLVED IN DEHYDRATION-RESPONSIVE EXPRESSION OF THE rd22 GENE IN ARABIDOPSIS

    ABE Hiroshi, URAO Takeshi, YAMAGUCHI-SHINOZAKI Kazuko, IWASAKI Toshisuke, HOSOKAWA Daijiro, SHINOZAKI Kazuo

    38   s59   1997.3

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  • イネ光応答性SRP1/importin様蛋白質の核局在化配列への結合

    岩崎 俊介, 徳富(西尾) 光恵, 山本 直樹

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   60   278 - 278   1996.10

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  • Isolation of farnesyl pyrophosphoric acid synthase (FPPS) cDNA of rice plant and light response expression.

    三宮一宰, 岩崎俊介, 松岡信, 宮尾光恵, 山本直樹

    日本植物生理学会年会要旨集   36th(1996)   140   1996.3

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  • CLONING OF A LIGHT-RESPONSIVE cDNA FROM RICE WHICH ENCODES A SRP1/IMPORTIN-LIKE PROTEIN

    IWASAKI Toshisuke, MIYAO Mitsue, YAMAMOTO Naoki

    37   64 - 64   1996.3

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  • Cloning of a rice cDNA coding famesyl pyrophosphate synthase and thelight-induced expression in etiolated leaves.

    三宮一宰, 岩崎俊介, 松岡信, 宮尾光恵, 山本直樹

    日本分子生物学会年会プログラム・講演要旨集   18th   326   1995.11

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  • 針葉樹カラマツの暗所芽生えにおける光非依存型クロロフィル合成遺伝子(chlB)の役割

    笠井 誠, 山田 恭司, 岩崎 俊介, 徳富(宮尾) 光恵, 山本 直樹

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   59   222 - 222   1995.9

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  • イネ黄化芽ばえの光応答型遺伝子群の解析

    岩崎 俊介, 笠井 誠, 斎藤 陽子, 徳富(宮尾) 光恵, 山本 直樹

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   59   305 - 305   1995.9

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  • THE PRESENCE OF LIGHT-INDEPENDENTCHLOROPHYLL SYNTHESIS-RELATED GENE (chlL) AND ETIOLATION IN LARCH

    KASAI Makoto, YAMADA Kyoji, IWASAKI Toshisuke, MIYAO Mitue, YAMAMOTO Naoki

    36   S96   1995.3

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  • A Variety of Function of MYB-Related Transcription Factors in Plants

    URAO Takeshi, IWASAKI Toshisuke, SHINOZAKI Kazuko, SHINOZAKI Kazuo

    33 ( 1 )   22 - 28   1995.1

  • 植物プロテインキナーゼの多様な機能

    溝口剛, 林田信明, 平山隆志, 浦尾剛, 岩崎俊介, 篠崎一雄

    蛋白質核酸酵素   39 ( 12 )   2131 - 2149   1994.9

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  • 植物ホルモンとストレス応答

    岩崎俊介, 篠崎和子, 篠崎一雄

    植物細胞工学   6 ( 3 )   191 - 198   1994.5

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  • CHARACTERIZATION OF GENES RESPONSIVE TO DESICCATION AND THEIR EXPRESSION IN ARABIDOPSIS-THALIANA

    K SHINOZAKI, K YAMAGUCHISHINOZAKI, T KIYOSUE, T IWASAKI, S URAO

    PHOTOSYNTHESIS RESEARCH   34 ( 1 )   217 - 217   1992.10

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  • 2Ba-4 陽イオン荷電ビーズを用いたタバコ原形質膜の単離

    大橋 祐子, 岩崎 俊介, 建部 到

    日本植物生理学会年会とシンポジウム講演要旨集   25   175 - 175   1985.3

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Research Projects

  • 植物単離核を用いた試験管内核タンパク質輸送実験系の確立

    2022.6 - 2023.3

    公益財団法人 内田エネルギー科学振興財団  試験研究費助成  生物系

    岩崎俊介

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  • イネ葉身角度を制御する重力刺激受容メカニズムの解析

    2005

    内田エネルギー科学振興財団  試験研究助成 

    岩崎 俊介

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\500000 ( Indirect Cost:\500000 )

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  • イネ葉身角度を制御する重力刺激受容メカニズムの解析

    2004

    内田エネルギー科学振興財団  試験研究助成 

    岩崎 俊介

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\500000 ( Indirect Cost:\500000 )

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  • Light-regulated gene expression of rice brassinosteroid-biosynthetic genes

    2001 - 2003

    The Sumitomo Foundation  Grant for Basic Science Research Projects 

    Toshisuke Iwasaki

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1000000 ( Direct Cost: \1000000 )

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  • Functional analysis of a rice importin-α binding protein

    Grant number:13640643  2001 - 2003

    Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research(基盤研究(C))  基盤研究(C)

    Toshisuke IWASAKI

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    Grant amount:\3600000 ( Direct Cost: \3600000 )

    To identify novel nuclear proteins that are involved in plants' responses to light environment, the author analyzed the function of a candidate protein, named IABP4, that had been isolated by its binding to rice importin-α1a(IMPα1a), a component of nuclear transport machinery, whose expression is strong in the dark and is down-regulated by light. The obtained results are as follows.1.Gene expression and the effect of light : Similar to rice IMPα1a gene, the expression of IABP4 was down-regulated by light both in etiolated-and green-seedlings of rice and Arabidopsis. The result suggests that...

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  • Functional analysis of rice proteins which bind to the α subunit of nuclear transport complex.

    Grant number:11640645  1999 - 2000

    Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research(基盤研究(C))  基盤研究(C)

    Toshisuke IWASAKI

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    Grant amount:\3600000 ( Direct Cost: \3600000 )

    The purpose of this research is to identify a novel nuclear protein which might be involved in the light-response in rice plants, by isolating proteins that bind to rice importin α 1a (IMP α 1a) whose expression is down-regulated by light. Below are the results.1. Analysis of an IMP α 1a-binding protein, IABP4, which has been isolated by cDNA screening by Far western method.(1) Determination of the full cDNA sequence : The IABP4 cDNA contained the entire coding sequence for a novel protein of predicted molecular mass of 122 kDa, and the homologous gene was found on the chromosome 2 of Arabi...

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  • イネの光応答発現を示す核移行シグナル受容体蛋白質のリガンドの探索

    Grant number:09740593  1997 - 1998

    文部科学省  科学研究費補助金(奨励研究(A))  奨励研究(A)

    岩崎俊介

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    Grant amount:\2100000 ( Direct Cost: \2100000 )

    本研究は、イネの負の光応答発現を示す核移行シグナル受容体蛋白質(AD752)に特異的に結合する蛋白質を単離することにより、光応答の制御に関わる可能性のある、未知の核蛋白質を同定することを目的としている。今年度に実施した研究実績の概要は下記のとおりである。1. イネの2種類の核移行シグナル受容体の蛋白質レベルでの発現の解析前年度は、それぞれの蛋白質に対する抗体を用いて、様々な組織由来のイネ粗抽出液についてウエスタンブロットを行ったが、いずれの抗体によっても非特異的だと思われる複数のバンドが検出され、抗体の特異性に問題があると考えられた。今年度は、抗体をアフィニティ-クロマトグラフィ-などにより精製し、この問題を解決する予定であったが、下記の実験に力を注いだため、新たな進展は得られなかった。2. 負の光応答発現を示す核移行シグナル受容体(AD752)に特異的に結合するイネ蛋白質のcDNAの単離前年度、蛋白質間相互作用を利用したスクリ-ニング法によって、イネ緑葉由来のcDNAライブラリ-から得られたcDNAクロ-ンは逆鎖が翻訳されたア-ティファクトであることが判明した。今年度は、黄化葉由来のライブラリ-から、新たに約4kbのサイズのcDNAクロ-ンを得た。その部分塩基配列から、マウスの核局在リン酸化蛋白質であるTSP(TPR-containing,SH2-binding phosp...

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  • ヒャクニチソウ単離葉肉細胞におけるDNA合成の制御

    1988 - 1989

    日本学術振興会  奨励研究(A) 

    岩崎 俊介

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Teaching Experience

  • 理学スタディ・スキルズ

    2022
    Institution name:新潟大学

  • 生物学実習I

    2022
    Institution name:新潟大学

  • 生物学基礎実習b

    2021
    Institution name:新潟大学

  • 生物学基礎演習

    2021
    Institution name:新潟大学

  • 台湾スプリングセミナー I

    2021
    Institution name:新潟大学

  • 台湾スプリングセミナー II

    2021
    Institution name:新潟大学

  • 植物機能制御論III

    2021
    Institution name:新潟大学

  • 生物学実験

    2021
    Institution name:新潟大学

  • 英語論文作成・発表演習

    2020
    Institution name:新潟大学

  • 課題研究II(生物学)

    2018
    Institution name:新潟大学

  • 自然科学基礎実験

    2018
    Institution name:新潟大学

  • 理学スタディ・スキルズ

    2018
    -
    2020
    Institution name:新潟大学

  • 台湾スプリングセミナー II

    2018
    Institution name:新潟大学

  • 台湾スプリングセミナー I

    2018
    Institution name:新潟大学

  • 課題研究I(生物学)

    2017
    Institution name:新潟大学

  • 生物学基礎実習a

    2017
    Institution name:新潟大学

  • 生物化学II(理)

    2017
    Institution name:新潟大学

  • 生物学総合演習

    2017
    Institution name:新潟大学

  • 細胞・遺伝学実習

    2017
    Institution name:新潟大学

  • 専門力アクティブ・ラーニング

    2017
    -
    2018
    Institution name:新潟大学

  • 生物学基礎演習

    2017
    Institution name:新潟大学

  • 科学・技術と社会

    2016
    -
    2018
    Institution name:新潟大学

  • 自然科学総論Ⅳ

    2015
    Institution name:新潟大学

  • 基礎生物科学実習I

    2014
    -
    2021
    Institution name:新潟大学

  • 生物化学II

    2013
    -
    2016
    Institution name:新潟大学

  • 生命科学への招待(生物学学習法)

    2012
    Institution name:新潟大学

  • 生物学特論Ⅳ

    2012
    -
    2016
    Institution name:新潟大学

  • 課題研究I(生物学科)

    2012
    -
    2016
    Institution name:新潟大学

  • 生物化学I

    2012
    Institution name:新潟大学

  • 農と食の博士セミナー

    2010
    -
    2011
    Institution name:新潟大学

  • 植物生理学特論Ⅲ

    2008
    Institution name:新潟大学

  • 植物機能制御論Ⅲ

    2008
    -
    2018
    Institution name:新潟大学

  • 日本事情自然系A

    2008
    -
    2012
    Institution name:新潟大学

  • 生物学基礎A

    2007
    Institution name:新潟大学

  • 植物生理学II

    2007
    Institution name:新潟大学

  • 植物生理学演習

    2007
    Institution name:新潟大学

  • 基礎生物科学実習II

    2007
    -
    2021
    Institution name:新潟大学

  • 生物学実験 I

    2007
    -
    2019
    Institution name:新潟大学

  • 植物分子生理学実習

    2007
    -
    2016
    Institution name:新潟大学

  • 分子生物学実習

    2007
    -
    2016
    Institution name:新潟大学

  • 課題研究II

    2007
    -
    2014
    Institution name:新潟大学

  • 植物分子生理学

    2007
    -
    2011
    Institution name:新潟大学

  • 課題研究I

    2007
    -
    2011
    Institution name:新潟大学

  • 植物機能制御論III

    2007
    Institution name:新潟大学

  • 基礎植物学

    2007
    Institution name:新潟大学

  • 植物生理学特論III

    2007
    Institution name:新潟大学

▶ display all