Updated on 2025/07/06

写真a

 
OHAZAMA Atsushi
 
Organization
Academic Assembly Institute of Medicine and Dentistry SHIGAKU KEIRETU Professor
Graduate School of Medical and Dental Sciences Oral Life Science Oral Biological Science Professor
Title
Professor
External link

Degree

  • 博士(歯学) ( 1994.3   昭和大学 )

Research Areas

  • Life Science / Developmental biology

Research History (researchmap)

  • 新潟大学 歯学部 教授

    2016

      More details

  • 新潟大学 歯学部 准教授

    2013 - 2015

      More details

  • King’s College London (UK) principal investigator (PI)

    2006 - 2013

      More details

  • King’s College London (UK) Senior research fellow

    2005 - 2006

      More details

Research History

  • Niigata University   Graduate School of Medical and Dental Sciences Oral Life Science Oral Biological Science   Professor

    2016.1

  • Niigata University   Graduate School of Medical and Dental Sciences Oral Life Science Oral Biological Science   Associate Professor

    2013.11 - 2015.12

 

Papers

  • Wnt/β-catenin Promotes Cementum Apposition in Periodontal Regeneration Reviewed

    Y. Ono, M. Kaku, L. Thant, H. Iwama, M. Arai, M. Mizukoshi, A. Dobashi, M. Kitami, M.M. Taketo, A. Ohazama, I. Saito, K. Uoshima

    Journal of Dental Research   2024.11

     More details

    Publishing type:Research paper (scientific journal)   Publisher:SAGE Publications  

    Regeneration of periodontal tissue, particularly the cementum–periodontal ligament (PDL)–bone complex, has long been challenging because the differentiation kinetics of cells and the molecular pathways contributing to the regeneration process are largely unknown. We aimed to evaluate the cell behavior and molecular pathways that contribute to periodontal tissue regeneration in vivo. We analyzed the process of periodontal tissue regeneration through subrenal capsule transplantation of immediately extracted molars in mice. We showed that the regenerated periodontal tissue in the subrenal capsule was morphologically comparable to the intact periodontal tissue, with increased cellular cementum thickness in the apical region. Cell tracing analysis revealed that the cells comprising the regenerated periodontal tissue were derived from transplanted teeth and were indispensable for periodontal tissue regeneration, whereas recipient mouse-derived cells partly contributed to angiogenesis. Bioinformatics analysis based on the gene expression profile in the transplanted teeth indicated that Wnt/β-catenin signaling is involved in periodontal tissue regeneration, which was further confirmed through β-catenin immunohistochemistry. Moreover, the constitutive activation of β-catenin in the cells of transplanted teeth was found to promote accelerated cellular cementum apposition, while the conditional knockout of β-catenin in the cells of transplanted teeth suppressed cellular cementum apposition. Notably, the manipulation of Wnt/β-catenin signaling did not interfere with the bone–PDL–cementum complex, while endogenous osteoclast activity was affected in bone. Our results demonstrated the essential roles of endogenous PDL cells in periodontal tissue regeneration and that Wnt/β-catenin signaling is involved in this process, particularly cellular cementum apposition. Hence, controlling this pathway could promote cementum regeneration, which is a critical process for the regeneration of the cementum–PDL–bone complex. This study provides novel insights into cell behavior and signaling pathways that will advance practical periodontal tissue regeneration.

    DOI: 10.1177/00220345241286490

    researchmap

    Other Link: https://journals.sagepub.com/doi/full-xml/10.1177/00220345241286490

  • Cell-cell interaction determines cell fate of mesoderm-derived cell in tongue development through Hh signaling. Reviewed International journal

    Maiko Kawasaki, Katsushige Kawasaki, Finsa Tisna Sari, Takehisa Kudo, Jun Nihara, Madoka Kitamura, Takahiro Nagai, Vanessa Utama, Yoko Ishida, Fumiya Meguro, Alex Kesuma, Akira Fujita, Takayuki Nishimura, Yuan Kogure, Satoshi Maruyama, Jun-Ichi Tanuma, Yoshito Kakihara, Takeyasu Maeda, Sarah Ghafoor, Roman H Khonsari, Pierre Corre, Paul T Sharpe, Martyn Cobourne, Brunella Franco, Atsushi Ohazama

    eLife   13   2024.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Dysfunction of primary cilia leads to genetic disorder, ciliopathies, which shows various malformations in many vital organs such as brain. Multiple tongue deformities including cleft, hamartoma, and ankyloglossia are also seen in ciliopathies, which yield difficulties in fundamental functions such as mastication and vocalization. Here, we found these tongue anomalies in mice with mutation of ciliary protein. Abnormal cranial neural crest-derived cells (CNCC) failed to evoke Hh signal for differentiation of mesoderm-derived cells into myoblasts, which resulted in abnormal differentiation of mesoderm-derived cells into adipocytes. The ectopic adipose subsequently arrested tongue swelling formation. Ankyloglossia was caused by aberrant cell migration due to lack of non-canonical Wnt signaling. In addition to ciliopathies, these tongue anomalies are often observed as non-familial condition in human. We found that these tongue deformities could be reproduced in wild-type mice by simple mechanical manipulations to disturb cellular processes which were disrupted in mutant mice. Our results provide hints for possible future treatment in ciliopathies.

    DOI: 10.7554/eLife.85042

    PubMed

    researchmap

  • Homozygosity for a Rare Plec Variant Suggests a Contributory Role in Congenital Insensitivity to Pain. Reviewed International journal

    Piranit Kantaputra, Teerada Daroontum, Kantapong Kitiyamas, Panat Piyakhunakorn, Katsushige Kawasaki, Achara Sathienkijkanchai, Pornswan Wasant, Nithiwat Vatanavicharn, Thippawan Yasanga, Massupa Kaewgahya, Sissades Tongsima, Timothy C Cox, Stefan T Arold, Atsushi Ohazama, Chumpol Ngamphiw

    International journal of molecular sciences   25 ( 12 )   2024.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Congenital insensitivity to pain is a rare human condition in which affected individuals do not experience pain throughout their lives. This study aimed to identify the molecular etiology of congenital insensitivity to pain in two Thai patients. Clinical, radiographic, histopathologic, immunohistochemical, and molecular studies were performed. Patients were found to have congenital insensitivity to pain, self-mutilation, acro-osteolysis, cornea scars, reduced temperature sensation, tooth agenesis, root maldevelopment, and underdeveloped maxilla and mandible. The skin biopsies revealed fewer axons, decreased vimentin expression, and absent neurofilament expression, indicating lack of dermal nerves. Whole exome and Sanger sequencing identified a rare homozygous variant c.4039C>T; p.Arg1347Cys in the plakin domain of Plec, a cytolinker protein. This p.Arg1347Cys variant is in the spectrin repeat 9 region of the plakin domain, a region not previously found to harbor pathogenic missense variants in other plectinopathies. The substitution with a cysteine is expected to decrease the stability of the spectrin repeat 9 unit of the plakin domain. Whole mount in situ hybridization and an immunohistochemical study suggested that Plec is important for the development of maxilla and mandible, cornea, and distal phalanges. Additionally, the presence of dental anomalies in these patients further supports the potential involvement of Plec in tooth development. This is the first report showing the association between the Plec variant and congenital insensitivity to pain in humans.

    DOI: 10.3390/ijms25126358

    PubMed

    researchmap

  • Genetic Variants in KCTD1 Are Associated with Isolated Dental Anomalies. Reviewed International journal

    Cholaporn Ruangchan, Chumpol Ngamphiw, Annop Krasaesin, Narin Intarak, Sissades Tongsima, Massupa Kaewgahya, Katsushige Kawasaki, Phitsanu Mahawong, Kullaya Paripurana, Bussaneeya Sookawat, Peeranat Jatooratthawichot, Timothy C Cox, Atsushi Ohazama, James R Ketudat Cairns, Thantrira Porntaveetus, Piranit Kantaputra

    International journal of molecular sciences   25 ( 10 )   2024.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    KCTD1 plays crucial roles in regulating both the SHH and WNT/β-catenin signaling pathways, which are essential for tooth development. The objective of this study was to investigate if genetic variants in KCTD1 might also be associated with isolated dental anomalies. We clinically and radiographically investigated 362 patients affected with isolated dental anomalies. Whole exome sequencing identified two unrelated families with rare (p.Arg241Gln) or novel (p.Pro243Ser) variants in KCTD1. The variants segregated with the dental anomalies in all nine patients from the two families. Clinical findings of the patients included taurodontism, unseparated roots, long roots, tooth agenesis, a supernumerary tooth, torus palatinus, and torus mandibularis. The role of Kctd1 in root development is supported by our immunohistochemical study showing high expression of Kctd1 in Hertwig epithelial root sheath. The KCTD1 variants in our patients are the first variants found to be located in the C-terminal domain, which might disrupt protein-protein interactions and/or SUMOylation and subsequently result in aberrant WNT-SHH-BMP signaling and isolated dental anomalies. Functional studies on the p.Arg241Gln variant are consistent with an impact on β-catenin levels and canonical WNT signaling. This is the first report of the association of KCTD1 variants and isolated dental anomalies.

    DOI: 10.3390/ijms25105179

    PubMed

    researchmap

  • CACNA1S mutation-associated dental anomalies: A calcium channelopathy. Reviewed International journal

    P Kantaputra, A Butali, S Eliason, C Chalkley, S Nakornchai, C Bongkochwilawan, K Kawasaki, A Kumchiang, C Ngamphiw, S Tongsima, J R Ketudat Cairns, B Olsen, W Intachai, A Ohazama, A S Tucker, B A Amendt

    Oral diseases   30 ( 3 )   1350 - 1359   2024.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVES: To identify the molecular etiology of distinct dental anomalies found in eight Thai patients and explore the mutational effects on cellular functions. MATERIALS AND METHODS: Clinical and radiographic examinations were performed for eight patients. Whole exome sequencing, mutant protein modelling, qPCR, western blot analysis, scratch assays, immunofluorescence, confocal analysis, in situ hybridization, and scanning electron micrography of teeth were done. RESULTS: All patients had molars with multiple supernumerary cusps, single-cusped premolars, and a reduction in root number. Mutation analysis highlighted a heterozygous c.865A>G; p.Ile289Val mutation in CACNA1S in the patients. CACNA1S is a component of the slowly inactivating L-type voltage-dependent calcium channel. Mutant protein modeling suggested that the mutation might allow leakage of Ca2+ or other cations, or a tightening, to restrict calcium flow. Immunohistochemistry analysis showed expression of Cacna1s in the developing murine tooth epithelium during stages of crown and root morphogenesis. In cell culture, the mutation resulted in abnormal cell migration of transfected CHO cells compared to wildtype CACNA1S, with changes to the cytoskeleton and markers of focal adhesion. CONCLUSIONS: The malformations observed in our patients suggest a role for calcium signaling in organization of both cusps and roots, affecting cell dynamics within the dental epithelium.

    DOI: 10.1111/odi.14551

    PubMed

    researchmap

  • Loss of Autophagy Disrupts Stemness of Ameloblast-Lineage Cells in Aging. Reviewed International journal

    H Ida-Yonemochi, K Otsu, T Irié, A Ohazama, H Harada, H Ohshima

    Journal of dental research   220345231209931 - 220345231209931   2023.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Autophagy is one of the intracellular degradation pathways and maintains cellular homeostasis, regulating the stress response, cell proliferation, and signal transduction. To elucidate the role of autophagy in the maintenance of dental epithelial stem cells and the subsequent enamel formation, we analyzed autophagy-deficient mice in epithelial cells (Atg7f/f;KRT14-Cre mice), focusing on the influence of aging and stress environments. We also performed in vitro cell and organ culture experiments with an autophagy inhibitor. In young Atg7f/f;KRT14-Cre mice, morphological change was not obvious in maxillary incisors, except for the remarkable cell death in the stratum intermedium of the transitional stage. However, under stress conditions of hyperglycemia, the incisor color changed to white in diabetes Atg7f/f;KRT14-Cre mice. Regarding dental epithelial stem cells, the shape of the apical bud region of the incisor became irregular with age, and odontoma was formed in aged Atg7f/f;KRT14-Cre mice. In addition, the shape of apical bud culture cells of Atg7f/f;KRT14-Cre mice became irregular and enlarged atypically, with epigenetic changes during culture, suggesting that autophagy deficiency may induce tumorigenesis in dental epithelial cells. The epigenetic change and upregulation of p21 expression were induced by autophagy inhibition in vivo and in vitro. These findings suggest that autophagy is important for the regulation of stem cell maintenance, proliferation, and differentiation of ameloblast-lineage cells, and an autophagy disorder may induce tumorigenesis in odontogenic epithelial cells.

    DOI: 10.1177/00220345231209931

    PubMed

    researchmap

  • A Mutation in CACNA1S Is Associated with Multiple Supernumerary Cusps and Root Maldevelopment. Reviewed International journal

    Piranit Kantaputra, Niramol Leelaadisorn, Athiwat Hatsadaloi, Natalina Quarto, Worrachet Intachai, Sissades Tongsima, Katsushige Kawasaki, Atsushi Ohazama, Chumpol Ngamphiw, Paswach Wiriyakijja

    Diagnostics (Basel, Switzerland)   13 ( 5 )   2023.2

     More details

    Language:English  

    BACKGROUND: Enamel knots and Hertwig epithelial root sheath (HERS) regulate the growth and folding of the dental epithelium, which subsequently determines the final form of tooth crown and roots. We would like to investigate the genetic etiology of seven patients affected with unique clinical manifestations, including multiple supernumerary cusps, single prominent premolars, and single-rooted molars. METHODS: Oral and radiographic examination and whole-exome or Sanger sequencing were performed in seven patients. Immunohistochemical study during early tooth development in mice was performed. RESULTS: A heterozygous variant (c. 865A>G; p.Ile289Val) in CACNA1S was identified in all the patients, but not in an unaffected family member and control. Immunohistochemical study showed high expression of Cacna1s in the secondary enamel knot. CONCLUSIONS: This CACNA1S variant seemed to cause impaired dental epithelial folding; too much folding in the molars and less folding in the premolars; and delayed folding (invagination) of HERS, which resulted in single-rooted molars or taurodontism. Our observation suggests that the mutation in CACNA1S might disrupt calcium influx, resulting in impaired dental epithelium folding, and subsequent abnormal crown and root morphology.

    DOI: 10.3390/diagnostics13050895

    PubMed

    researchmap

  • Ift88 regulates enamel formation via involving Shh signaling. Reviewed International journal

    Takehisa Kudo, Maiko Kawasaki, Katsushige Kawasaki, Fumiya Meguro, Jun Nihara, Izumi Honda, Madoka Kitamura, Akira Fujita, Kazuaki Osawa, Kaya Ichikawa, Takahiro Nagai, Yoko Ishida, Paul T Sharpe, Takeyasu Maeda, Isao Saito, Atsushi Ohazama

    Oral diseases   29 ( 4 )   1622 - 1631   2022.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVES: The ciliopathies are a wide spectrum of human diseases, which are caused by perturbations in the function of primary cilia. Tooth enamel anomalies are often seen in ciliopathy patients; however, the role of primary cilia in enamel formation remains unclear. MATERIALS AND METHODS: We examined mice with epithelial conditional deletion of the ciliary protein, Ift88, (Ift88fl / fl ;K14Cre). RESULTS: Ift88fl / fl ;K14Cre mice showed premature abrasion in molars. A pattern of enamel rods which is determined at secretory stage, was disorganized in Ift88 mutant molars. Many amelogenesis-related molecules expressing at the secretory stage, including amelogenin and ameloblastin, enamelin, showed significant downregulation in Ift88 mutant molar tooth germs. Shh signaling is essential for amelogenesis, which was found to be downregulated in Ift88 mutant molar at the secretory stage. Application of Shh signaling agonist at the secretory stage partially rescued enamel anomalies in Ift88 mutant mice. CONCLUSION: Findings in the present study indicate that the function of the primary cilia via Ift88 is critical for the secretory stage of amelogenesis through involving Shh signaling.

    DOI: 10.1111/odi.14162

    PubMed

    researchmap

  • Expression of R-spondins/Lgrs in development of movable craniofacial organs. Reviewed International journal

    Jun Nihara, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Fumiya Meguro, Takehisa Kudo, Supaluk Trakanant, Takahiro Nagai, Isao Saito, Takeyasu Maeda, Atsushi Ohazama

    Gene expression patterns : GEP   41   119195 - 119195   2021.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Wnt signaling plays a critical role in the development of many organs, including the major movable craniofacial organs tongue, lip, and eyelid. Four members of the R-spondin family (Rspo1-4) bind to Lgr4/5/6 to regulate the activation of Wnt signaling. However, it is not fully understood how Rspos/Lgrs regulate Wnt signaling during the development of movable craniofacial organs. To address this question, we examined the expression of Rspos, Lgrs, and Axin2 (major mediator of canonical Wnt signaling) during tongue, lip, and eyelid development. The expression of Axin2, Rspos and Lgrs was observed in many similar regions, suggesting that Rspos likely activate canonical Wnt signaling through the Lgr-dependent pathway in these regions. Lgr expression was not detected in regions where Axin2 and Rspos were expressed, suggesting that Rspos might activate canonical Wnt signaling through the Lgr-independent pathway in these regions. In addition, the expression of Rspos and Lgrs were observed in some other regions where Axin2 was not expressed, suggesting the possibility that Rspos and/or Lgrs are involved in non-canonical Wnt signaling or the Wnt-independent pathway. Thus, we identified a dynamic spatiotemporal expression pattern of Rspos and Lgrs during the development of the eyelid, tongue, and lip.

    DOI: 10.1016/j.gep.2021.119195

    PubMed

    researchmap

  • Perivascular Hedgehog responsive cells play a critical role in peripheral nerve regeneration via controlling angiogenesis. Reviewed International journal

    Yurie Yamada, Jun Nihara, Supaluk Trakanant, Takehisa Kudo, Kenji Seo, Izumi Iida, Kenji Izumi, Masayuki Kurose, Yutaka Shimomura, Miho Terunuma, Takeyasu Maeda, Atsushi Ohazama

    Neuroscience research   173   62 - 70   2021.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Hh signaling has been shown to be activated in intact and injured peripheral nerve. However, the role of Hh signaling in peripheral nerve is not fully understood. In the present study, we observed that Hh signaling responsive cells [Gli1(+) cells] in both the perineurium and endoneurium. In the endoneurium, Gli1(+) cells were classified as blood vessel associated or non-associated. After injury, Gli1(+) cells around blood vessels mainly proliferated to then accumulate into the injury site along with endothelial cells. Hh signaling activity was retained in Gli1(+) cells during nerve regeneration. To understand the role of Hedgehog signaling in Gli1(+) cells during nerve regeneration, we examined mice with Gli1(+) cells-specific inactivation of Hh signaling (Smo cKO). After injury, Smo cKO mice showed significantly reduced numbers of accumulated Gli1(+) cells along with disorganized vascularization at an early stage of nerve regeneration, which subsequently led to an abnormal extension of the axon. Thus, Hh signaling in Gli1(+) cells appears to be involved in nerve regeneration through controlling new blood vessel formation at an early stage.

    DOI: 10.1016/j.neures.2021.06.003

    PubMed

    researchmap

  • MicroRNAs regulate distal region of mandibular development through Hh signaling Reviewed

    Supaluk Trakanant, Jun Nihara, Takahiro Nagai, Maiko Kawasaki, Katsushige Kawasaki, Yoko Ishida, Fumiya Meguro, Takehisa Kudo, Akane Yamada, Takeyasu Maeda, Isao Saito, Atsushi Ohazama

    JOURNAL OF ANATOMY   238 ( 3 )   711 - 719   2021.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/joa.13328

    Web of Science

    researchmap

  • A novel P3H1 mutation is associated with osteogenesis imperfecta type VIII and dental anomalies. Reviewed International journal

    Piranit Nik Kantaputra, Prapai Dejkhamron, Worrachet Intachai, Chumpol Ngamphiw, James R Ketudat Cairns, Katsushige Kawasaki, Atsushi Ohazama, Bjorn Olsen, Sissades Tongsima, Salita Angkurawaranon

    Oral surgery, oral medicine, oral pathology and oral radiology   132 ( 6 )   4564 - 4564   2021.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: Our objective was to investigate the molecular etiology of osteogenesis imperfecta type VIII and dental anomalies in 4 siblings of a Karen tribe family. MATERIALS AND METHODS: Four patients and their unaffected parents were studied by clinical and radiographic examination. In situ hybridization of P3h1 during early murine tooth development, whole-exome sequencing, and Sanger direct sequencing were performed. RESULTS: A novel homozygous missense P3H1 mutation (NM_001243246.1; c.2141A>G; NP_001230175.1; p.Lys714Arg) was identified in all patients. Their unaffected parents were heterozygous for the mutation. The mutation is hypothesized to belong to isoform c of P3H1. Mutations in P3H1 are associated with autosomal recessive osteogenesis imperfecta type VIII. Hypodontia, a mesiodens, and single-rooted permanent second molars found in our patients have never been reported in patients with P3H1 mutations. Single-rooted second permanent molars or failure to form multiple roots implies effects of the P3H1 mutation on root development. CONCLUSIONS: We report a novel P3H1 mutation as the underlying cause of osteogenesis imperfecta type VIII with dental anomalies. Our study suggests that isoform c of P3H1 is also a functional isoform of P3H1. We report, for the first time, to our knowledge, the association of P3H1 mutation and osteogenesis imperfecta type VIII with dental anomalies.

    DOI: 10.1016/j.oooo.2021.01.023

    PubMed

    researchmap

  • R2TP/PAQosome as a promising chemotherapeutic target in cancer. Reviewed International journal

    Yoshito Kakihara, Tetsuo Kiguchi, Atsushi Ohazama, Makio Saeki

    The Japanese dental science review   56 ( 1 )   38 - 42   2020.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    R2TP/PAQosome (particle for arrangement of quaternary structure) is a novel multisubunit chaperone specialized in the assembly/maturation of protein complexes that are involved in essential cellular processes such as PIKKs (phosphatidylinositol 3-kinase-like kinases) signaling, snoRNP (small nucleolar ribonucleoprotein) biogenesis, and RNAP II (RNA polymerase II) complex formation. In this review article, we describe the current understanding of R2TP/PAQosome functions and characteristics as well as how the chaperone complex is involved in oncogenesis, highlighting DNA damage response, mTOR (mammalian target of rapamycin) pathway as well as snoRNP biogenesis. Also, we discuss its possible involvement in HNSCC (head and neck squamous cell carcinoma) including OSCC (oral squamous cell carcinoma). Finally, we provide an overview of current anti-cancer drug development efforts targeting R2TP/PAQosome.

    DOI: 10.1016/j.jdsr.2019.08.001

    PubMed

    researchmap

  • Changes in signalling pathways in the palatal cleft in CL/Fr mice Reviewed

    Akane Yamada, Takahiro Nagai, Atsushi Kitamura, Maiko Kawasaki, Katsushige Kawasaki, Yasumitsu Kodama, Takeyasu Maeda, Atsushi Ohazama, Ritsuo Takagi

    JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY MEDICINE AND PATHOLOGY   32 ( 5 )   331 - 335   2020.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ajoms.2019.12.001

    Web of Science

    researchmap

  • Overactivation of the NF-κB pathway impairs molar enamel formation. Reviewed International journal

    Akane Yamada, Maiko Kawasaki, Yasuo Miake, Yurie Yamada, James Blackburn, Kataushige Kawasaki, Supaluk Trakanant, Takahiro Nagai, Jun Nihara, Takehisa Kudo, Fumiya Meguro, Ruth Schmidt-Ullrich, Bigang Liu, Yinling Hu, Angustias Page, Ángel Ramírez, Paul T Sharpe, Takeyasu Maeda, Ritsuo Takagi, Atsushi Ohazama

    Oral diseases   26 ( 7 )   1513 - 1522   2020.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: Hypohidrotic ectodermal dysplasia (HED) is a hereditary disorder characterized by abnormal structures and functions of the ectoderm-derived organs, including teeth. HED patients exhibit a variety of dental symptoms, such as hypodontia. Although disruption of the EDA/EDAR/EDARADD/NF-κB pathway is known to be responsible for HED, it remains unclear whether this pathway is involved in the process of enamel formation. EXPERIMENTAL SUBJECTS AND METHODS: To address this question, we examined the mice overexpressing Ikkβ (an essential component required for the activation of NF-κB pathway) under the keratin 5 promoter (K5-Ikkβ). RESULTS: Upregulation of the NF-κB pathway was confirmed in the ameloblasts of K5-Ikkβ mice. Premature abrasion was observed in the molars of K5-Ikkβ mice, which was accompanied by less mineralized enamel. However, no significant changes were observed in the enamel thickness and the pattern of enamel rods in K5-Ikkβ mice. Klk4 expression was significantly upregulated in the ameloblasts of K5-Ikkβ mice at the maturation stage, and the expression of its substrate, amelogenin, was remarkably reduced. This suggests that abnormal enamel observed in K5-Ikkβ mice was likely due to the compromised degradation of enamel protein at the maturation stage. CONCLUSION: Therefore, we could conclude that the overactivation of the NF-κB pathway impairs the process of amelogenesis.

    DOI: 10.1111/odi.13384

    PubMed

    researchmap

  • Gli3 is a Key Factor in the Schwann Cells from Both Intact and Injured Peripheral Nerves. Reviewed International journal

    Yurie Yamada, Supaluk Trakanant, Jun Nihara, Takehisa Kudo, Kenji Seo, Makio Saeki, Masayuki Kurose, Daisuke Matsumaru, Takeyasu Maeda, Atsushi Ohazama

    Neuroscience   432   229 - 239   2020.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Hedgehog (Hh) signaling has been shown to be involved in regulating both intact and injured peripheral nerves. Therefore, it is critical to understand how Hh signaling is regulated in the peripheral nerve. One of the transcription factors of the Hh signaling pathway, Gli3, functions as both a repressor and an activator of Hh signaling activity. However, it remains unclear whether Gli3 is involved in controlling the intact and/or injured peripheral nerves. We found that Gli3 act as a repressor in the Schwann cells (SCs) of intact sciatic nerves. Although Dhh and Ptch1 expression were present, Hh signaling was not activated in these SCs. Moreover, heterozygous Gli3 mutation (Gli3-/+) induced ectopic Hh signaling activity in SCs. Hh signaling was thus suppressed by Gli3 in the SCs of intact sciatic nerves. Minor morphological changes were observed in the intact nerves from Gli3-/+ mice. Gli3 expression was significantly decreased following injury and ligand expression switched from Dhh to Shh, which activated Hh signaling in SCs from wild-type mice. Changes of these ligands was found to be important for nerve regeneration in which the downregulation of Gli3 was also involved. In fact, Gli3-/+ mice exhibited accelerated ligand switching and subsequent nerve regeneration. Both suppression of Hh signaling with Gli3 in the intact nerves and activation of Hh signaling without Gli3 in the injured nerve were observed in the SCs in an autocrine manner. Thus, Gli3 is a key factor in the control of intact peripheral nerve homeostasis and nerve regeneration.

    DOI: 10.1016/j.neuroscience.2020.02.036

    PubMed

    researchmap

  • Juberg-Hayward syndrome is a cohesinopathy, caused by mutation in ESCO2. Reviewed International journal

    Piranit Nik Kantaputra, Prapai Dejkhamron, Worrachet Intachai, Chumpol Ngamphiw, Katsushige Kawasaki, Atsushi Ohazama, Suttichai Krisanaprakornkit, Bjorn Olsen, Sissades Tongsima, Jame R Ketudat Cairns

    European journal of orthodontics   43 ( 1 )   45 - 50   2020.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Juberg-Hayward syndrome (JHS; MIM 216100) is a rare autosomal recessive malformation syndrome, characterized by cleft lip/palate, microcephaly, ptosis, short stature, hypoplasia or aplasia of thumbs, and dislocation of radial head and fusion of humerus and radius leading to elbow restriction. OBJECTIVE: To report for the first time the molecular aetiology of JHS. PATIENT AND METHODS: Clinical and radiographic examination, whole exome sequencing, Sanger sequencing, mutant protein model construction, and in situ hybridization of Esco2 expression in mouse embryos were performed. RESULTS: Clinical findings of the patient consisted of repaired cleft lip/palate, microcephaly, ptosis, short stature, delayed bone age, hypoplastic fingers and thumbs, clinodactyly of the fifth fingers, and humeroradial synostosis leading to elbow restriction. Intelligence is normal. Whole exome sequencing of the whole family showed a novel homozygous base substitution c.1654C>T in ESCO2 of the proband. The sister was homozygous for the wildtype variant. Parents were heterozygous for the mutation. The mutation is predicted to cause premature stop codon p.Arg552Ter. Mutations in ESCO2, a gene involved in cohesin complex formation, are known to cause Roberts/SC phocomelia syndrome. Roberts/SC phocomelia syndrome and JHS share similar clinical findings, including autosomal recessive inheritance, short stature, cleft lip/palate, severe upper limb anomalies, and hypoplastic digits. Esco2 expression during the early development of lip, palate, eyelid, digits, upper limb, and lower limb and truncated protein model are consistent with the defect. CONCLUSIONS: Our study showed that Roberts/SC phocomelia syndrome and JHS are allelic and distinct entities. This is the first report demonstrating that mutation in ESCO2 causes JHS, a cohesinopathy.

    DOI: 10.1093/ejo/cjaa023

    PubMed

    researchmap

  • Clouston syndrome with pili canaliculi, pili torti, overgrown hyponychium, onycholysis, taurodontism and absence of palmoplantar keratoderma. Reviewed International journal

    Piranit Kantaputra, Worrachet Intachai, Katsushige Kawasaki, Atsushi Ohazama, Bruce Carlson, Natalina Quarto, Chulabhorn Pruksachatkun, Mati Chuamanochan

    The Journal of dermatology   47 ( 6 )   e230-e232   2020.3

     More details

  • Molecular mechanisms in palatal rugae development. Reviewed International journal

    Supaluk Trakanant, Jun Nihara, Maiko Kawasaki, Fumiya Meguro, Akane Yamada, Katsushige Kawasaki, Isao Saito, Maeda Takeyasu, Atsushi Ohazama

    Journal of oral biosciences   62 ( 1 )   30 - 35   2020.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Periodic patterning of iterative structures is diverse across the animal kingdom. Clarifying the molecular mechanisms involved in the formation of these structures helps to elucidate the genetic commonality of developmental processes, as organs with these structures are believed to share the same molecular mechanisms and fundamental processes. Palatal rugae are periodic corrugated structures on the hard palate and are conserved in all mammals. Although the numbers and patterns of the palatal rugae are species specific, they are consistent in each mammalian species, except humans. HIGHLIGHT: Palatal rugae development is thus under strict genetic control in most mammals and is an excellent model to investigate the genetic commonality of developmental processes to form periodic patterning. CONCLUSION: This review highlights the current understanding of the molecular mechanisms of palatal rugae development.

    DOI: 10.1016/j.job.2019.12.002

    PubMed

    researchmap

  • Ift88 is involved in mandibular development. Reviewed International journal

    Atsushi Kitamura, Maiko Kawasaki, Katsushige Kawasaki, Fumiya Meguro, Akane Yamada, Takahiro Nagai, Yasumitsu Kodama, Supaluk Trakanant, Paul T Sharpe, Takeyasu Maeda, Ritsuo Takagi, Atsushi Ohazama

    Journal of anatomy   236 ( 2 )   317 - 324   2020.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The mandible is a crucial organ in both clinical and biological fields due to the high frequency of congenital anomalies and the significant morphological changes during evolution. Primary cilia play a critical role in many biological processes, including the determination of left/right axis patterning, the regulation of signaling pathways, and the formation of bone and cartilage. Perturbations in the function of primary cilia are known to cause a wide spectrum of human diseases: the ciliopathies. Craniofacial dysmorphologies, including mandibular deformity, are often seen in patients with ciliopathies. Mandibular development is characterized by chondrogenesis and osteogenesis; however, the role of primary cilia in mandibular development is not fully understood. To address this question, we generated mice with mesenchymal deletions of the ciliary protein, Ift88 (Ift88fl/fl ;Wnt1Cre). Ift88fl/fl ;Wnt1Cre mice showed ectopic mandibular bone formation, whereas Ift88 mutant mandible was slightly shortened. Meckel's cartilage was modestly expanded in Ift88fl/fl ;Wnt1Cre mice. The downregulation of Hh signaling was found in most of the mesenchyme of Ift88 mutant mandible. However, mice with a mesenchymal deletion of an essential molecule for Hh signaling activity, Smo (Smofl/fl ;Wnt1Cre), showed only ectopic mandibular formation, whereas Smo mutant mandible was significantly shortened. Ift88 is thus involved in chondrogenesis and osteogenesis during mandibular development, partially through regulating Sonic hedgehog (Shh) signaling.

    DOI: 10.1111/joa.13096

    PubMed

    researchmap

  • Primary cilia in murine palatal rugae development. Reviewed International journal

    Mayuko Nakaniwa, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Fumiya Meguro, Maeda Takeyasu, Atsushi Ohazama

    Gene expression patterns : GEP   34   119062 - 119062   2019.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Periodic patterning of iterative structures is a fundamental process during embryonic development, since these structures are diverse across the animal kingdom. Therefore, elucidating the molecular mechanisms in the formation of these structures promotes understanding of the process of organogenesis. Periodically patterned ridges, palatal rugae (situated on the hard palate of mammals), are an excellent experimental model to clarify the molecular mechanisms involved in the formation of periodic patterning of iterative structures. Primary cilia are involved in many biological events, including the regulation of signaling pathways such as Shh and non-canonical Wnt signaling. However, the role of primary cilia in the development of palatal rugae remains unclear. We found that primary cilia were localized to the oral cavity side of the interplacode epithelium of the palatal rugae, whereas restricted localization of primary cilia could not be detected in other regions. Next, we generated mice with a placodal conditional deletion of the primary cilia protein Ift88, using ShhCre mice (Ift88 fl/fl;ShhCre). Highly disorganized palatal rugae were observed in Ift88 fl/fl;ShhCre mice. Furthermore, by comparative in situ hybridization analysis, many Shh and non-canonical Wnt signaling-related molecules showed spatiotemporal expression patterns during palatal rugae development, including restricted expression in the epithelium (placodes and interplacodes) and mesenchyme. Some of these expression were found to be altered in Ift88 fl/fl;ShhCre mice. Primary cilia is thus involved in development of palatal rugae.

    DOI: 10.1016/j.gep.2019.119062

    PubMed

    researchmap

  • Bmp signaling in molar cusp formation. Reviewed International journal

    Fumiya Meguro, Thantrira Porntaveetus, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Yoshito Kakihara, Makio Saeki, Koichi Tabeta, John A Kessler, Takeyasu Maeda, Atsushi Ohazama

    Gene expression patterns : GEP   32   67 - 71   2019.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Tooth cusp is a crucial structure, since the shape of the molar tooth is determined by number, shape, and size of the cusp. Bone morphogenetic protein (Bmp) signaling is known to play a critical role in tooth development, including in initiation. However, it remains unclear whether Bmp signaling is also involved in cusp formation. To address this question, we examined cusp in two different transgenic mouse lines: mice with overexpression of Bmp4 (K14-Bmp4), and those with Bmp inhibitor, Noggin, (K14-Noggin) under keratin14 (K14) promoter. K14-Noggin mice demonstrated extra cusps, whereas reduced number of cusps was observed in K14-Bmp4 mice. To further understand how Bmps are expressed during cusp formation, we performed whole-mount in situ hybridisation analysis of three major Bmps (Bmp2, Bmp4, and Bmp7) in murine maxillary and mandibular molars from E14.5 to P3. The linear expressions of Bmp2 and Bmp4 were observed in both maxillary and mandibular molars at E14.5. The expression patterns of Bmp2 and Bmp4 became significantly different between the maxillary and mandibular molars at E16.5. At P3, all Bmps were expressed in all the cusp regions of the maxillary molar; however, the patterns differed. All Bmps thus exhibited dynamic temporo-spatial expression during the cusp formation. It could therefore be inferred that Bmp signaling is involved in regulating cusp formation.

    DOI: 10.1016/j.gep.2019.04.002

    PubMed

    researchmap

  • Ift88 limits bone formation in maxillary process through suppressing apoptosis. Reviewed International journal

    Momoko Watanabe, Maiko Kawasaki, Katsushige Kawasaki, Atsushi Kitamura, Takahiro Nagai, Yasumitsu Kodama, Fumiya Meguro, Akane Yamada, Paul T Sharpe, Takeyasu Maeda, Ritsuo Takagi, Atsushi Ohazama

    Archives of oral biology   101   43 - 50   2019.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: The development of the maxillary bone is under strict molecular control because of its complicated structure. Primary cilia play a critical role in craniofacial development, since defects in primary cilia are known to cause congenital craniofacial dysmorphologies as a wide spectrum of human diseases: the ciliopathies. The primary cilia also are known to regulate bone formation. However, the role of the primary cilia in maxillary bone development is not fully understood. DESIGN: To address this question, we generated mice with a mesenchymal conditional deletion ofIft88 using the Wnt1Cre mice (Ift88fl/fl;Wnt1Cre). The gene Ift88 encodes a protein that is required for the function and formation of primary cilia. RESULTS: It has been shown thatIft88fl/fl;Wnt1Cre mice exhibit cleft palate. Here, we additionally observed excess bone formation in the Ift88 mutant maxillary process. We also found ectopic apoptosis in the Ift88 mutant maxillary process at an early stage of development. To investigate whether the ectopic apoptosis is related to the Ift88 mouse maxillary phenotypes, we generated Ift88fl/fl;Wnt1Cre;p53-/- mutants to reduce apoptosis. The Ift88fl/fl;Wnt1Cre;p53-/- mice showed no excess bone formation, suggesting that the cells evading apoptosis by the presence of Ift88 in wild-type mice limit bone formation in maxillary development. On the other hand, the palatal cleft was retained in the Ift88fl/fl;Wnt1Cre;p53-/- mice, indicating that the excess bone formation or abnormal apoptosis was independent of the cleft palate phenotype in Ift88 mutant mice. CONCLUSIONS: Ift88 limits bone formation in the maxillary process by suppressing apoptosis.

    DOI: 10.1016/j.archoralbio.2019.02.017

    PubMed

    researchmap

  • ADAMTSL1 and mandibular prognathism. Reviewed International journal

    Piranit N Kantaputra, Apitchaya Pruksametanan, Nattapol Phondee, Athiwat Hutsadaloi, Worrachet Intachai, Katsushig Kawasaki, Atsushi Ohazama, Chumpol Ngamphiw, Sissades Tongsima, James R Ketudat Cairns, Polbhat Tripuwabhrut

    Clinical genetics   95 ( 4 )   507 - 515   2019.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Mandibular prognathism is characterized by a prognathic or prominent mandible. The objective of this study was to find the gene responsible for mandibular prognathism. Whole exome sequencing analysis of a Thai family (family 1) identified the ADAMTSL1 c.176C>A variant as the potential defect. We cross-checked our exome data of 215 people for rare variants in ADAMTSL1 and found that the c.670C>G variant was associated with mandibular prognathism in families 2 and 4. Mutation analysis of ADAMTSL1 in 79 unrelated patients revealed the c.670C>G variant was also found in family 3. We hypothesize that mutations in ADAMTSL1 cause failure to cleave aggrecan in the condylar cartilage, and that leads to overgrowth of the mandible. Adamtsl1 is strongly expressed in the condensed mesenchymal cells of the mouse condyle, but not at the cartilage of the long bones. This explains why the patients with ADAMTSL1 mutations had abnormal mandibles but normal long bones. This is the first report that mutations in ADAMTSL1 are responsible for the pathogenesis of mandibular prognathism.

    DOI: 10.1111/cge.13519

    PubMed

    researchmap

  • Semaphorin 3A Inhibits Nerve Regeneration During Early Stage after Inferior Alveolar Nerve Transection. Reviewed International journal

    Hiroko Kanemaru, Yurie Yamada, Atsushi Ohazama, Takeyasu Maeda, Kenji Seo

    Scientific reports   9 ( 1 )   4245 - 4245   2019.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Neuroma formation at sites of injury can impair peripheral nerve regeneration. Although the involvement of semaphorin 3A has been suggested in neuroma formation, this detailed process after injury is not fully understood. This study was therefore undertaken to examine the effects of semaphorin 3A on peripheral nerve regeneration during the early stage after injury. Immunohistochemistry for semaphorin 3A and PGP9.5, a general neuronal marker, was carried out for clarify chronological changes in their expressions after transection of the mouse inferior alveolar nerve thorough postoperative days 1 to 7. At postoperative day 1, the proximal stump of the damaged IAN exhibited semaphorin 3A, while the distal stump lacked any immunoreactivity. From this day on, its expression lessened, ultimately disappearing completely in all regions of the transected inferior alveolar nerve. A local administration of an antibody to semaphorin 3A into the nerve transection site at postoperative day 3 inhibited axon sprouting at the injury site. This antibody injection increased the number of trigeminal ganglion neurons labeled with DiI (paired t-test, p < 0.05). Immunoreactivity of the semaphorin 3A receptor, neuropilin-1, was also detected at the proximal stump at postoperative day 1. These results suggest that nerve injury initiates semaphorin 3A production in ganglion neurons, which is then delivered through the nerve fibers to the proximal end, thereby contributes to the inhibition of axonal sprouting from the proximal region of injured nerves in the distal direction. To our knowledge, this is the first report to reveal the involvement of Sema3A in the nerve regeneration process at its early stage.

    DOI: 10.1038/s41598-018-37819-6

    PubMed

    researchmap

  • MicroRNAs control eyelid development through regulating Wnt signaling. Reviewed International journal

    Takahiro Nagai, Supaluk Trakanant, Maiko Kawasaki, Katsushige Kawasaki, Yurie Yamada, Momoko Watanabe, James Blackburn, Yoko Otsuka-Tanaka, Mitsue Hishinuma, Atsushi Kitatmura, Fumiya Meguro, Akane Yamada, Yasumitsu Kodama, Takeyasu Maeda, Qiliang Zhou, Yasuo Saijo, Akihiro Yasue, Paul T Sharpe, Robert Hindges, Ritsuo Takagi, Atsushi Ohazama

    Developmental dynamics : an official publication of the American Association of Anatomists   248 ( 3 )   201 - 210   2019.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: The timing, location, and level of gene expression are crucial for normal organ development, because morphogenesis requires strict genetic control. MicroRNAs (miRNAs) are noncoding small single-stranded RNAs that play a critical role in regulating gene expression level. Although miRNAs are known to be involved in many biological events, the role of miRNAs in organogenesis is not fully understood. Mammalian eyelids fuse and separate during development and growth. In mice, failure of this process results in the eye-open at birth (EOB) phenotype. RESULTS: It has been shown that conditional deletion of mesenchymal Dicer (an essential protein for miRNA processing; Dicer fl/fl ;Wnt1Cre) leads to the EOB phenotype with full penetrance. Here, we identified that the up-regulation of Wnt signaling resulted in the EOB phenotype in Dicer mutants. Down-regulation of Fgf signaling observed in Dicer mutants was caused by an inverse relationship between Fgf and Wnt signaling. Shh and Bmp signaling were down-regulated as the secondary effects in Dicer fl/fl ;Wnt1Cre mice. Wnt, Shh, and Fgf signaling were also found to mediate the epithelial-mesenchymal interactions in eyelid development. CONCLUSIONS: miRNAs control eyelid development through Wnt. Developmental Dynamics 248:201-210, 2019. © 2019 Wiley Periodicals, Inc.

    DOI: 10.1002/dvdy.10

    PubMed

    researchmap

  • The Sonic Hedgehog signaling pathway regulates inferior alveolar nerve regeneration. Reviewed International journal

    Yurie Yamada, Atsushi Ohazama, Takeyasu Maeda, Kenji Seo

    Neuroscience letters   671   114 - 119   2018.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.neulet.2017.12.051

    Web of Science

    PubMed

    researchmap

  • Application of Hypoxic Exposure Combined with Osteogenic Induction for the Enhancement of Multiple Osteoinductive Capabilities in Rat Mesenchymal Cells Reviewed

    Naoaki Saito, Hiroko Kato, Yosuke Akiba, Yuko Hara, Taku Kojima, Michiko Yoshizawa, Atsushi Ohazama, Takeyasu Maeda, Tadaharu Kobayashi, Kenji Izumi

    Open Journal of Stomatology   8 ( 2 )   53 - 69   2018.2

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.4236/ojst.2018.82005

    researchmap

  • Expanding the Oro-Dental and Mutational Spectra of Kabuki Syndrome and Expression of KMT2D and KDM6A in Human Tooth Germs. Reviewed International journal

    Thantrira Porntaveetus, Mushriq F Abid, Thanakorn Theerapanon, Chalurmpon Srichomthong, Atsushi Ohazama, Katsushige Kawasaki, Maiko Kawasaki, Kanya Suphapeetiporn, Paul T Sharpe, Vorasuk Shotelersuk

    International journal of biological sciences   14 ( 4 )   381 - 389   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.7150/ijbs.23517

    Web of Science

    PubMed

    researchmap

  • Lrp4/Wise regulates palatal rugae development through Turing-type reaction-diffusion mechanisms. Reviewed International journal

    Maiko Kawasaki, Katsushige Kawasaki, Fumiya Meguro, Akane Yamada, Ryuichi Ishikawa, Thantrira Porntaveetus, James Blackburn, Yoko Otsuka-Tanaka, Naoaki Saito, Masato S Ota, Paul T Sharpe, John A Kessler, Joachim Herz, Martyn T Cobourne, Takeyasu Maeda, Atsushi Ohazama

    PloS one   13 ( 9 )   e0204126   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Periodic patterning of iterative structures is diverse across the animal kingdom. Clarifying the molecular mechanisms involved in the formation of these structure helps to elucidate the process of organogenesis. Turing-type reaction-diffusion mechanisms have been shown to play a critical role in regulating periodic patterning in organogenesis. Palatal rugae are periodically patterned ridges situated on the hard palate of mammals. We have previously shown that the palatal rugae develop by a Turing-type reaction-diffusion mechanism, which is reliant upon Shh (as an inhibitor) and Fgf (as an activator) signaling for appropriate organization of these structures. The disturbance of Shh and Fgf signaling lead to disorganized palatal rugae. However, the mechanism itself is not fully understood. Here we found that Lrp4 (transmembrane protein) was expressed in a complementary pattern to Wise (a secreted BMP antagonist and Wnt modulator) expression in palatal rugae development, representing Lrp4 expression in developing rugae and Wise in the inter-rugal epithelium. Highly disorganized palatal rugae was observed in both Wise and Lrp4 mutant mice, and these mutants also showed the downregulation of Shh signaling, which was accompanied with upregulation of Fgf signaling. Wise and Lrp4 are thus likely to control palatal rugae development by regulating reaction-diffusion mechanisms through Shh and Fgf signaling. We also found that Bmp and Wnt signaling were partially involved in this mechanism.

    DOI: 10.1371/journal.pone.0204126

    PubMed

    researchmap

  • Sox Genes Show Spatiotemporal Expression during Murine Tongue and Eyelid Development. Reviewed International journal

    Ryuichi Ishikawa, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Supaluk Trakanant, Fumiya Meguro, Atsushi Kitamura, Takehisa Kudo, Takeyasu Maeda, Atsushi Ohazama

    International journal of dentistry   2018 ( 10 )   1601363 - 1601363   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The tongue is a critical organ, involved in functions such as speaking, swallowing, mastication, and degustation. Although Sox genes are known to play critical roles in many biological processes, including organogenesis, the expression of the Sox family members during tongue development remains unclear. We therefore performed a comparative in situ hybridization analysis of 17 Sox genes (Sox1-14, 17, 18, and 21) during murine tongue development. Sox2, 4, 6, 8, 9, 10, 11, 12, and 21 were found to be expressed in the tongue epithelium, whereas Sox2, 4-6, 8-11, 13, and 21 showed expression in the mesenchyme of the developing tongue. Expression of Sox1, 4, 6, 8-12, and 21 were observed in the developing tongue muscle. Sox5 and 13 showed expression only at E12, while Sox1 expression was observed only on E18. Sox6, 8, 9, and 12 showed expression at several stages. Although the expression of Sox2, 4, 10, 11, and 21 was detected during all the four stages of tongue development, their expression patterns differed among the stages. We thus identified a dynamic spatiotemporal expression pattern of the Sox genes during murine tongue development. To understand whether Sox genes are involved in the development of other craniofacial organs through similar roles to those in tongue development, we also examined the expression of Sox genes in eyelid primordia, which also contain epithelium, mesenchyme, and muscle. However, expression patterns and timing of Sox genes differed between tongue and eyelid development. Sox genes are thus related to organogenesis through different functions in each craniofacial organ.

    DOI: 10.1155/2018/1601363

    PubMed

    researchmap

  • Vascularization via activation of VEGF-VEGFR signaling is essential for peripheral nerve regeneration. Reviewed

    Yohei Nishida, Yurie Yamada, Hiroko Kanemaru, Atsushi Ohazama, Takeyasu Maeda, Kenji Seo

    Biomedical research (Tokyo, Japan)   39 ( 6 )   287 - 294   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Peripheral nerve injury has been suggested to up-regulate mRNA for the vascular endothelial growth factor (VEGF) which enhances nerve regeneration. VEGF is known to regulate angiogenesis by binding with a specific receptor, the vascular endothelial growth factor receptor (VEGFR). However, little is known about the involvement of VEGF-VEGFR signaling in the nerve regeneration at early stages though previous studies contained a lengthy observation. The present study examined that relationship between angiogenesis and peripheral nerve regeneration at the early stage after nerve transection by focusing on the chronological changes in the expression patterns of VEGF-VEGFR signaling. This study used our previously reported experimental model for nerve regeneration following the transection of the inferior alveolar nerve (IAN) in mice. In a double staining of PGP9.5 and CD31, respective markers for the nerve fibers and endothelial cells, CD31 immunoreactions first appeared in the injury site on postoperative (PO) day 2 when the transected nerve fibers had not been re-connected. The most intense immunoreaction for CD31 was found around the regenerating nerve fibers extending from the proximal stump on PO day 3, but it gradually lessened to disappear by PO day 7. The expression patterns of VEGFR1 and VEGFR2 showed similar chronological changes through the observation periods, with most intense immunoreaction found on PO day 3. Western blotting of total protein extracted from the injury site demonstrated the clear bands for VEGF-A and VEGF-B on PO day 2, indicating a time lag for the expression of ligands and receptors. A local administration of antibody to VEGF-A inhibited the elongation of the nerve fibers from the proximal stump. Furthermore, this administration of VEGF-A antibody inhibited the expression of CD31 in the gap between proximal and distal stumps. These results indicated that a nerve injury initiates productions in VEGF-A and VEFG-B, followed with the expression of VEGFR1 and VEGFR2 at early stages after the nerve injury. Taken these findings together, it is reasonable to postulate that immediate response of VEGF-VEGFR signaling to nerve injury plays a crucial role in local angiogenesis, resulting in a trigger for the regeneration of the nerve fibers in mouse IAN.

    DOI: 10.2220/biomedres.39.287

    PubMed

    researchmap

  • Al-Awadi-Raas-Rothschild syndrome with dental anomalies and a novel WNT7A mutation. Reviewed International journal

    Piranit Nik Kantaputra, Seema Kapoor, Prashant Verma, Massupa Kaewgahya, Katsushige Kawasaki, Atsushi Ohazama, James R Ketudat Cairns

    European journal of medical genetics   60 ( 12 )   695 - 700   2017.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ejmg.2017.09.005

    Web of Science

    PubMed

    researchmap

  • The effect of bone mass and architecture on mandibular condyle after mandibular distraction. Reviewed International journal

    Daisuke Suda, Atsushi Ohazama, Takeyasu Maeda, Tadaharu Kobayashi

    Oral surgery, oral medicine, oral pathology and oral radiology   124 ( 4 )   339 - 347   2017.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.oooo.2017.05.472

    Web of Science

    PubMed

    researchmap

  • A novel GJA1 mutation in oculodentodigital dysplasia with extensive loss of enamel Reviewed

    T. Porntaveetus, C. Srichomthong, A. Ohazama, K. Suphapeetiporn, V. Shotelersuk

    ORAL DISEASES   23 ( 6 )   795 - 800   2017.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/odi.12663

    Web of Science

    PubMed

    researchmap

  • TFAP2B mutation and dental anomalies. Reviewed International journal

    Natchaya Tanasubsinn, Rekwan Sittiwangkul, Yupada Pongprot, Katsushige Kawasaki, Atsushi Ohazama, Thanapat Sastraruji, Massupa Kaewgahya, Piranit Nik Kantaputra

    Journal of human genetics   62 ( 8 )   769 - 775   2017.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/jhg.2017.37

    Web of Science

    PubMed

    researchmap

  • Regional Regulation of Filiform Tongue Papillae Development by Ikk alpha/Irf6 Reviewed

    Maiko Kawasaki, Katsushige Kawasaki, Shelly Oommen, James Blackburn, Momoko Watanabe, Takahiro Nagai, Atsushi Kitamura, Takeyasu Maeda, Bigang Liu, Ruth Schmidt-Ullrich, Taishin Akiyama, Jun-Ichiro Inoue, Nigel L. Hammond, Paul T. Sharpe, Atsushi Ohazama

    DEVELOPMENTAL DYNAMICS   245 ( 9 )   937 - 946   2016.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/DVDY.24427

    Web of Science

    PubMed

    researchmap

  • Spatio-temporal expression of Sox genes in murine palatogenesis Reviewed

    Momoko Watanabe, Katsushige Kawasaki, Maiko Kawasaki, Thantrira Portaveetus, Shelly Oommen, James Blackburn, Takahiro Nagai, Atsushi Kitamura, Atsushi Nishikawa, Yasumitsu Kodama, Ritsuo Takagi, Takeyasu Maeda, Paul T. Sharpe, Atsushi Ohazama

    GENE EXPRESSION PATTERNS   21 ( 2 )   111 - 118   2016.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.gep.2016.05.002

    Web of Science

    PubMed

    researchmap

  • Disrupted mitochondrial function in the Opa3(L122P) mouse model for Costeff Syndrome impairs skeletal integrity Reviewed

    Alice E. Navein, Esther J. Cooke, Jennifer R. Davies, Terence G. Smith, Lois H. M. Wells, Atsushi Ohazama, Christopher Healy, Paul T. Sharpe, Sam L. Evans, Bronwen A. J. Evans, Marcela Votruba, Timothy Wells

    HUMAN MOLECULAR GENETICS   25 ( 12 )   2404 - 2416   2016.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/hmg/ddw107

    Web of Science

    PubMed

    researchmap

  • Contribution of synovial lining cells to synovial vascularization of the rat temporomandibular joint Reviewed

    Kayoko Nozawa-Inoue, Fumiko Harada, Jin Magara, Atsushi Ohazama, Takeyasu Maeda

    JOURNAL OF ANATOMY   228 ( 3 )   520 - 529   2016.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/joa.12426

    Web of Science

    PubMed

    researchmap

  • GREMLIN 2 Mutations and Dental Anomalies Reviewed

    P. N. Kantaputra, M. Kaewgahya, A. Hatsadaloi, P. Vogel, K. Kawasaki, A. Ohazama, J. R. Ketudat Cairns

    JOURNAL OF DENTAL RESEARCH   94 ( 12 )   1646 - 1652   2015.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0022034515608168

    Web of Science

    PubMed

    researchmap

  • Craniofacial Development and Growth in Polycystic Kidney Disease Reviewed

    Atsushi Ohazama, Paul T. Sharpe

    2015.11

     More details

    Language:English   Publisher:Codon Publications  

    Autosomal dominant polycystic kidney disease (ADPKD) is an inherited disorder characterized by the presence of multiple cysts in kidneys. ADPKD has been shown to be caused by mutations in the genes of PKD1 and PKD2, encoding polycystin-1 (PC1) and polycystin-2 (PC2), respectively. Polycystins are localized in primary cilia that play roles in multiple biological processes including mechanoreception, Ca2+ influx and cell signalling pathways. Primary cilia are known to play important roles in regulating craniofacial development and growth. In this chapter, we summarize the function of Pkd1 and Pkd2 in controlling mouse craniofacial development and growth, and discuss PKD-associated molecular mechanisms.

    DOI: 10.15586/codon.pkd.2015

    PubMed

    researchmap

  • Excess NF-kappa B Induces Ectopic Odontogenesis in Embryonic Incisor Epithelium Reviewed

    J. Blackburn, K. Kawasaki, T. Porntaveetus, M. Kawasaki, Y. Otsuka-Tanaka, Y. Miake, M. S. Ota, M. Watanabe, M. Hishinuma, T. Nomoto, S. Oommen, S. Ghafoor, F. Harada, K. Nozawa-Inoue, T. Maeda, R. Peterkova, H. Lesot, J. Inoue, T. Akiyama, R. Schmidt-Ullrich, B. Liu, Y. Hu, A. Page, A. Ramirez, P. T. Sharpe, A. Ohazama

    JOURNAL OF DENTAL RESEARCH   94 ( 1 )   121 - 128   2015.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0022034514556707

    Web of Science

    PubMed

    researchmap

  • Expression of Sox genes in tooth development Reviewed

    Katsushige Kawasaki, Maiko Kawasaki, Momoko Watanabe, Erik Idrus, Takahiro Nagai, Shelly Oommen, Takeyasu Maeda, Nobuko Hagiwara, Jianwen Que, Paul T. Sharpe, Atsushi Ohazama

    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY   59 ( 10-12 )   471 - 478   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1387/ijdb.150192ao

    Web of Science

    PubMed

    researchmap

  • Root dentin anomaly and a PLG mutation Reviewed

    Napaporn Tananuvat, Pimlak Charoenkwan, Atsushi Ohazama, James R. Ketuda Cairns, Massupa Kaewgahya, Piranit Nik Kantaputra

    EUROPEAN JOURNAL OF MEDICAL GENETICS   57 ( 11-12 )   630 - 635   2014.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ejmg.2014.09.006

    Web of Science

    PubMed

    researchmap

  • Submicron Imaging of Soft-Tissues Using Low-Dose Phase-Contrast X-Ray Synchrotron Microtomography with an Iodine Contrast Agent Reviewed

    R. H. Khonsari, C. Healy, A. Ohazama, P. T. Sharpe, R. H. Khonsari, H. Dutel, H. Dutel, C. Charles, L. Viriot, P. Tafforeau

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY   297 ( 10 )   1803 - 1807   2014.10

     More details

  • ラット顎関節滑膜におけるデスミン免疫陽性B型およびRECA-1免疫陽性A型表層細胞の血管形成への関与

    野澤 佳世子[井上], 真柄 仁, 河野 芳朗, 大峡 淳, 前田 健康

    Journal of Oral Biosciences Supplement   2014   193 - 193   2014.9

     More details

    Language:Japanese   Publisher:(一社)歯科基礎医学会  

    researchmap

  • Enamel-Renal-Gingival Syndrome, Hypodontia, and a Novel FAM20A Mutation Reviewed

    Piranit Nik Kantaputra, Chotika Bongkochwilawan, Massupa Kaewgahya, Atsushi Ohazama, Hulya Kayserili, Arzu Pinar Erdem, Oya Aktoren, Yeliz Guven

    AMERICAN JOURNAL OF MEDICAL GENETICS PART A   164 ( 8 )   2124 - 2128   2014.8

     More details

  • R-spondins/Lgrs expression in tooth development Reviewed

    Maiko Kawasaki, Thantrira Porntaveetus, Katsushige Kawasaki, Shelly Oommen, Yoko Otsuka-Tanaka, Mitsue Hishinuma, Takato Nomoto, Takeyasu Maeda, Keiyo Takubo, Toshio Suda, Paul T. Sharpe, Atsushi Ohazama

    DEVELOPMENTAL DYNAMICS   243 ( 6 )   844 - 851   2014.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dvdy.24124

    Web of Science

    PubMed

    researchmap

  • PKA regulatory subunit expression in tooth development Reviewed

    Silvia Ferreira de Sousa, Katsushige Kawasaki, Maiko Kawasaki, Ana Angelova Volponi, Ricardo Santiago Gomez, Carolina Cavalieri Gomes, Paul T. Sharpe, Atsushi Ohazama

    GENE EXPRESSION PATTERNS   15 ( 1 )   46 - 51   2014.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.gep.2014.04.002

    Web of Science

    PubMed

    researchmap

  • Shape and Volume of Craniofacial Cavities in Intentional Skull Deformations Reviewed

    R. H. Khonsari, M. Friess, J. Nysjo, G. Odri, F. Malmberg, I. Nystrom, E. Messo, J. M. Hirsch, E. A. M. Cabanis, K. H. Kunzelmann, J. M. Salagnac, P. Corre, A. Ohazama, P. T. Sharpe, P. Charlier, R. Olszewski

    AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY   151 ( 1 )   110 - 119   2013.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/ajpa.22263

    Web of Science

    PubMed

    researchmap

  • A mathematical model for mechanotransduction at the early steps of suture formation Reviewed

    R. H. Khonsari, J. Olivier, P. Vigneaux, S. Sanchez, P. Tafforeau, P. E. Ahlberg, F. Di Rocco, D. Bresch, P. Corre, A. Ohazama, P. T. Sharpe, V. Calvez

    PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES   280 ( 1759 )   20122670   2013.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1098/rspb.2012.2670

    Web of Science

    PubMed

    researchmap

  • Multiple postnatal craniofacial anomalies are characterized by conditional loss of polycystic kidney disease 2 (Pkd2) Reviewed

    Roman H. Khonsari, Atsushi Ohazama, Ramin Raouf, Maiko Kawasaki, Katsushige Kawasaki, Thantrira Porntaveetus, Sarah Ghafoor, Peter Hammond, Michael Suttie, Guillaume A. Odri, Richard N. Sandford, John N. Wood, Paul T. Sharpe

    HUMAN MOLECULAR GENETICS   22 ( 9 )   1873 - 1885   2013.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/hmg/ddt041

    Web of Science

    PubMed

    researchmap

  • The buccohypophyseal canal is an ancestral vertebrate trait maintained by modulation in sonic hedgehog signaling Reviewed

    Roman H. Khonsari, Maisa Seppala, Alan Pradel, Hugo Dutel, Gael Clement, Oleg Lebedev, Sarah Ghafoor, Michaela Rothova, Abigael Tucker, John G. Maisey, Chen-Ming Fan, Maiko Kawasaki, Atsushi Ohazama, Paul Tafforeau, Brunella Franco, Jill Helms, Courtney J. Haycraft, Albert David, Philippe Janvier, Martyn T. Cobourne, Paul T. Sharpe

    BMC BIOLOGY   11   27   2013.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/1741-7007-11-27

    Web of Science

    PubMed

    researchmap

  • Oral lining mucosa development depends on mesenchymal microRNAs Reviewed

    Y. Otsuka-Tanaka, S. Oommen, M. Kawasaki, K. Kawasaki, N. Imam, F. Jalani-Ghazani, R. Hindges, P. T. Sharpe, A. Ohazama

    Journal of Dental Research   92 ( 3 )   229 - 234   2013.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0022034512470830

    Scopus

    PubMed

    researchmap

  • The origin of the stapes and relationship to the otic capsule and oval window Reviewed

    Hannah Thompson, Atsushi Ohazama, Paul T. Sharpe, Abigail S. Tucker

    DEVELOPMENTAL DYNAMICS   241 ( 9 )   1396 - +   2012.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dvdy.23831

    Web of Science

    PubMed

    researchmap

  • Dyschromatosis Symmetrica Hereditaria With Long Hair on the Forearms, Hypo/Hyperpigmented Hair, and Dental Anomalies: Report of a Novel ADAR1 Mutation Reviewed

    Piranit Nik Kantaputra, Wannapa Chinadet, Atsushi Ohazama, Michihiro Kono

    AMERICAN JOURNAL OF MEDICAL GENETICS PART A   158A ( 9 )   2258 - 2265   2012.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/ajmg.a.35488

    Web of Science

    PubMed

    researchmap

  • Cytoplasmic Plaque Formation in Hemidesmosome Development Is Dependent on SoxF Transcription Factor Function Reviewed

    Shelly Oommen, Mathias Francois, Maiko Kawasaki, Melanie Murrell, Katsushige Kawasaki, Thantrira Porntaveetus, Sarah Ghafoor, Neville J. Young, Yoshimasa Okamatsu, John McGrath, Peter Koopman, Paul T. Sharpe, Atsushi Ohazama

    PLOS ONE   7 ( 9 )   e43857   2012.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0043857

    Web of Science

    PubMed

    researchmap

  • Distinct roles of MicroRNAs in epithelium and mesenchyme during tooth development Reviewed

    Shelly Oommen, Yoko Otsuka-Tanaka, Najam Imam, Maiko Kawasaki, Katsushige Kawasaki, Farnoosh Jalani-Ghazani, Angela Anderegg, Rajeshwar Awatramani, Robert Hindges, Paul T. Sharpe, Atsushi Ohazama

    DEVELOPMENTAL DYNAMICS   241 ( 9 )   1465 - 1472   2012.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dvdy.23828

    Web of Science

    PubMed

    researchmap

  • Wnt signaling in the murine diastema Reviewed

    Thantrira Porntaveetus, Atsushi Ohazama, Hong Y. Choi, Joachim Herz, Paul T. Sharpe

    EUROPEAN JOURNAL OF ORTHODONTICS   34 ( 4 )   518 - 524   2012.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/ejo/cjr049

    Web of Science

    PubMed

    researchmap

  • Bmp signalling in filiform tongue papillae development Reviewed

    Katsushige Kawasaki, Thantrira Porntaveetus, Shelly Oommen, Sarah Ghafoor, Maiko Kawasaki, Yoko Otsuka-Tanaka, James Blackburn, John A. Kessler, Paul T. Sharpe, Atsushi Ohazama

    ARCHIVES OF ORAL BIOLOGY   57 ( 6 )   805 - 813   2012.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.archoralbio.2011.11.014

    Web of Science

    PubMed

    researchmap

  • The role of Irf6 in tooth epithelial invagination Reviewed

    James Blackburn, Atsushi Ohazama, Katsushige Kawasaki, Yoko Otsuka-Tanaka, Bigang Liu, Kenya Honda, Ryan B. Rountree, Yinling Hu, Maiko Kawasaki, Walter Birchmeier, Ruth Schmidt-Ullrich, Akira Kinoshita, Brian C. Schutte, Nigel L. Hammond, Michael J. Dixon, Paul T. Sharpe

    DEVELOPMENTAL BIOLOGY   365 ( 1 )   61 - 70   2012.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ydbio.2012.02.009

    Web of Science

    PubMed

    researchmap

  • Long-Term Survival in Infantile Malignant Autosomal Recessive Osteopetrosis Secondary to Homozygous p.Arg526Gln Mutation in CLCN7 Reviewed

    Piranit Nik Kantaputra, Saranya Thawanaphong, Witchapong Issarangporn, Phennapha Klangsinsirikul, Atsushi Ohazama, Paul Sharpe, Chayarop Supanchart

    AMERICAN JOURNAL OF MEDICAL GENETICS PART A   158A ( 4 )   909 - 916   2012.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/ajmg.a.35264

    Web of Science

    PubMed

    researchmap

  • Periodic stripe formation by a Turing mechanism operating at growth zones in the mammalian palate Reviewed

    Andrew D. Economou, Atsushi Ohazama, Thantrira Porntaveetus, Paul T. Sharpe, Shigeru Kondo, M. Albert Basson, Amel Gritli-Linde, Martyn T. Cobourne, Jeremy B. A. Green

    NATURE GENETICS   44 ( 3 )   348 - U163   2012.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/ng.1090

    Web of Science

    PubMed

    researchmap

  • Histological analysis of the embryonic and adult tooth. Reviewed

    Ohazama A

    Methods in molecular biology (Clifton, N.J.)   887   1 - 13   2012

  • Developmental stalling and organ-autonomous regulation of morphogenesis Reviewed

    Isabelle Miletich, Wei-Yuan Yu, Ruofang Zhang, Kai Yang, Simone Caixeta de Andrade, Silvia Fontes do A. Pereira, Atsushi Ohazama, Orin B. Mock, Georg Buchner, Jane Sealby, Zoe Webster, Minglian Zhao, Marianna Bei, Paul T. Sharpe

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   108 ( 48 )   19270 - 19275   2011.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1112801108

    Web of Science

    PubMed

    researchmap

  • Podoplanin Is Regulated by AP-1 and Promotes Platelet Aggregation and Cell Migration in Osteosarcoma Reviewed

    Akiko Kunita, Takeshi G. Kashima, Atsushi Ohazama, Agamemnon E. Grigoriadis, Masashi Fukayama

    AMERICAN JOURNAL OF PATHOLOGY   179 ( 2 )   1041 - 1049   2011.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ajpath.2011.04.027

    Web of Science

    PubMed

    researchmap

  • The Smallest Teeth in the World are Caused by Mutations in the PCNT Gene Reviewed

    Piranit Kantaputra, Pranoot Tanpaiboon, Thantrira Porntaveetus, Atsushi Ohazama, Paul Sharpe, Anita Rauch, Atiwat Hussadaloy, Christian T. Thiel

    AMERICAN JOURNAL OF MEDICAL GENETICS PART A   155A ( 6 )   1398 - 1403   2011.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/ajmg.a.33984

    Web of Science

    PubMed

    researchmap

  • Expression of fibroblast growth factors (Fgfs) in murine tooth development Reviewed

    Thantrira Porntaveetus, Yoko Otsuka-Tanaka, M. Albert Basson, Anne M. Moon, Paul T. Sharpe, Atsushi Ohazama

    JOURNAL OF ANATOMY   218 ( 5 )   534 - 543   2011.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1469-7580.2011.01352.x

    Web of Science

    PubMed

    researchmap

  • Delayed Re-Epithelialization in Periostin-Deficient Mice during Cutaneous Wound Healing Reviewed

    Takashi Nishiyama, Isao Kii, Takeshi G. Kashima, Yoshinao Kikuchi, Atsushi Ohazama, Masashi Shimazaki, Masashi Fukayama, Akira Kudo

    PLOS ONE   6 ( 4 )   e18410   2011.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0018410

    Web of Science

    PubMed

    researchmap

  • Lrp4: A Novel Modulator of Extracellular Signaling in Craniofacial Organogenesis Reviewed

    Atsushi Ohazama, Thantrira Porntaveetus, Masato S. Ota, Joachim Herz, Paul T. Sharpe

    AMERICAN JOURNAL OF MEDICAL GENETICS PART A   152A ( 12 )   2974 - 2983   2010.12

     More details

  • Ectoderm, Endoderm, and the Evolution of Heterodont Dentitions Reviewed

    Atsushi Ohazama, Kim E. Haworth, Masato S. Ota, Roman H. Khonsari, Paul T. Sharpe

    GENESIS   48 ( 6 )   382 - 389   2010.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dvg.20634

    Web of Science

    PubMed

    researchmap

  • Expression of Fgf signalling pathway related genes during palatal rugae development in the mouse Reviewed

    Thantrira Porntaveetus, Shelly Oommen, Paul T. Sharpe, Atsushi Ohazama

    GENE EXPRESSION PATTERNS   10 ( 4-5 )   193 - 198   2010.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.gep.2010.03.004

    Web of Science

    PubMed

    researchmap

  • A role for suppressed incisor cuspal morphogenesis in the evolution of mammalian heterodont dentition Reviewed

    Atsushi Ohazama, James Blackburn, Thantrira Porntaveetus, Masato S. Ota, Hong Y. Choi, Eric B. Johnson, Philip Myers, Shelly Oommen, Kazuhiro Eto, John A. Kessler, Takashi Kondo, Gareth J. Fraser, Todd Streelman, Ulyses F. J. Pardinas, Abigail S. Tucker, Pablo E. Ortiz, Cyril Charles, Laurent Viriot, Joachim Herz, Paul T. Sharpe

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   107 ( 1 )   92 - 97   2010.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.0907236107

    Web of Science

    PubMed

    researchmap

  • Primary cilia regulate Shh activity in the control of molar tooth number Reviewed

    Atsushi Ohazama, Courtney J. Haycraft, Maisa Seppala, James Blackburn, Sarah Ghafoor, Martyn Cobourne, David C. Martinelli, Chen-Ming Fan, Renata Peterkova, Herve Lesot, Bradley K. Yoder, Paul T. Sharpe

    DEVELOPMENT   136 ( 6 )   897 - 903   2009.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/dev.027979

    Web of Science

    PubMed

    researchmap

  • Patterning of molar tooth roots in mammals Reviewed

    Masato S. Ota, Taka Nakahara, Yoriaki Kanri, Yukishige Kozawa, Atsushi Ohazama, Takaaki Aoba, Takashi Kondo, Sachiko Iseki

    Journal of Oral Biosciences   51 ( 4 )   193 - 198   2009

     More details

    Language:English   Publisher:Japanese Association for Oral Biology  

    DOI: 10.2330/joralbiosci.51.193

    Scopus

    researchmap

  • MAMMALIAN INCISORS RETAIN A CUSPAL MORPHOGENESIS PROGRAMME Reviewed

    James Blackburn, Atsushi Ohazama, Thantrira Porntaveetus, Joachim Herz, Paul Sharpe

    JOURNAL OF VERTEBRATE PALEONTOLOGY   29   65A - 65A   2009

     More details

    Language:English  

    Web of Science

    researchmap

  • Lrp4 Modulates Extracellular Integration of Cell Signaling Pathways in Development Reviewed

    Atsushi Ohazama, Eric B. Johnson, Masato S. Ota, Hong J. Choi, Thantrira Porntaveetus, Shelly Oommen, Nobuyuki Itoh, Kazuhiro Eto, Amel Gritli-Linde, Joachim Herz, Paul T. Sharpe

    PLOS ONE   3 ( 12 )   e4092   2008.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0004092

    Web of Science

    PubMed

    researchmap

  • TFII-I gene family during tooth development: Candidate genes for tooth anomalies in Williams syndrome Reviewed

    Atsushi Ohazama, Paul T. Sharpe

    DEVELOPMENTAL DYNAMICS   236 ( 10 )   2884 - 2888   2007.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dvdy.21311

    Web of Science

    PubMed

    researchmap

  • Expression of claudins in murine tooth development Reviewed

    Atsushi Ohazama, Paul T. Sharpe

    DEVELOPMENTAL DYNAMICS   236 ( 1 )   290 - 294   2007.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dvdy.21001

    Web of Science

    PubMed

    researchmap

  • Effects of combined treatment with porous bovine inorganic bone grafts and bilayer porcine collagen membrane on refractory one-wall intrabony defects Reviewed

    J Sakata, H Abe, A Ohazama, K Okubo, C Nagashima, M Suzuki, K Hasegawa

    INTERNATIONAL JOURNAL OF PERIODONTICS & RESTORATIVE DENTISTRY   26 ( 2 )   161 - 169   2006.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.11607/prd.00.0683

    Web of Science

    PubMed

    researchmap

  • Organized tooth-specific cellular differentiation stimulated by BMP4 Reviewed

    A Ohazama, A Tucker, PT Sharpe

    JOURNAL OF DENTAL RESEARCH   84 ( 7 )   603 - 606   2005.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/154405910508400704

    Web of Science

    PubMed

    researchmap

  • [The possibility of tooth regenerative therapy]. Reviewed

    Ohazama A

    Clinical Calcium   15 ( 7 )   81 - 85   2005.7

  • TNF signalling in tooth development Reviewed

    A Ohazama, PT Sharpe

    CURRENT OPINION IN GENETICS & DEVELOPMENT   14 ( 5 )   513 - 519   2004.10

  • Stem-cell-based tissue engineering of murine teeth Reviewed

    A Ohazama, SAC Modino, Miletich, I, PT Sharpe

    JOURNAL OF DENTAL RESEARCH   83 ( 7 )   518 - 522   2004.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/154405910408300702

    Web of Science

    PubMed

    researchmap

  • Opg, rank, and rankl in tooth development: Co-ordination of Odontogenesis and Osteogenesis Reviewed

    A Ohazama, JM Courtney, PT Sharpe

    JOURNAL OF DENTAL RESEARCH   83 ( 3 )   241 - 244   2004.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/154405910408300311

    Web of Science

    PubMed

    researchmap

  • A dual role for lkk alpha in tooth development Reviewed

    A Ohazama, YL Hu, R Schmidt-Ullrich, YX Cao, C Scheidereit, M Karin, PT Sharpe

    DEVELOPMENTAL CELL   6 ( 2 )   219 - 227   2004.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S1534-5807(04)00024-3

    Web of Science

    PubMed

    researchmap

  • Traf6 is essential for murine tooth cusp morphogenesis Reviewed

    A Ohazama, JM Courtney, AS Tucker, A Naito, S Tanaka, JI Inoue, PT Sharpe

    DEVELOPMENTAL DYNAMICS   229 ( 1 )   131 - 135   2004.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dvdy.10400

    Web of Science

    PubMed

    researchmap

  • Retention of allogenic calvarial periosteum as soft tissue around an allogenic transplanted tooth in alveolar bone Reviewed

    Kawasaki H, Okamatsu Y, Ohazama A

    Oral Bioscience & Medicine   1   123 - 134   2004

     More details

  • Overgrowth: Clinical Assessment of 15 Cases. Reviewed

    Takano T, Ohazama A, Ohtake T, Hasegawa K

    Journal of Showa University Dental Society   24   39 - 46   2004

  • Expression of TNF-receptor-associated factor genes in murine tooth development Reviewed

    A Ohazama, JM Courtney, PT Sharpe

    GENE EXPRESSION PATTERNS   3 ( 2 )   127 - 129   2003.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S1567-133X(03)00028-0

    Web of Science

    PubMed

    researchmap

  • Participation of endogenous IGF-I and TGF-beta 1 with enamel matrix derivative-stimulated cell growth in human periodontal ligament cells Reviewed

    K Okubo, M Kobayashi, T Takiguchi, T Takada, A Ohazama, Y Okamatsu, K Hasegawa

    JOURNAL OF PERIODONTAL RESEARCH   38 ( 1 )   1 - 9   2003.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1034/j.1600-0765.2003.01607.x

    Web of Science

    PubMed

    researchmap

  • Tooth development is independent of a Hox patterning programme Reviewed

    CT James, A Ohazama, AS Tucker, PT Sharpe

    DEVELOPMENTAL DYNAMICS   225 ( 3 )   332 - 335   2002.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dvdy.10168

    Web of Science

    PubMed

    researchmap

  • Participation of periodontal ligament cells with regeneration of alveolar bone Reviewed

    J Isaka, A Ohazama, M Kobayashi, C Nagashima, T Takiguchi, H Kawasaki, T Tachikawa, K Hasegawa

    JOURNAL OF PERIODONTOLOGY   72 ( 3 )   314 - 323   2001.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1902/jop.2001.72.3.314

    Web of Science

    PubMed

    researchmap

  • Periodontal tissue regeneration using fibrin tissue adhesive material. Reviewed

    Ohazama A, Hatayama J, Okamatsu Y, Isatsu K, Tachikawa T, Hasegawa K

    Periodontal Clinical Investigations   18   26 - 38   1996

▶ display all

MISC

  • ラット間葉系細胞の多面的骨誘導能に対する低酸素処理の効果に関する検討

    齋藤 直朗, 泉 健次, 秋葉 陽介, 加藤 寛子, 原 夕子, 小島 拓, 芳澤 享子, 小林 正治, 大峡 淳, 前田 健康

    新潟歯学会雑誌   45 ( 2 )   106 - 106   2015.12

     More details

    Language:Japanese   Publisher:新潟歯学会  

    researchmap

  • the buccohypophyseal canal is an ancestral vertebrate trait maintained by modulation in sonic hedgehog signaling (vol 11, 27, 2013)

    Roman H. Khonsari, Maisa Seppala, Alan Pradel, Hugo Dutel, Gael Clement, Oleg Lebedev, Sarah Ghafoor, Michaela Rothova, Abigael Tucker, John G. Maisey, Chen-Ming Fan, Atsushi Ohazama, Paul Tafforeau, Brunella Franco, Jill Helms, Courtney J. Haycraft, Albert David, Philippe Janvier, Martyn T. Cobourne, Paul T. Sharpe

    BMC BIOLOGY   11   2013.6

     More details

  • A dual role for IKK alpha tooth development

    P. T. Sharpe, K. E. Haworth, A. Ohazama

    JOURNAL OF DENTAL RESEARCH   82   B305 - B305   2003.6

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Is bone regeneration affected by presence of periodontal ligament?

    C Nagashima, A Ohazama, T Takiguchi, H Kawasaki, M Kobayashi, T Tachikawa, K Hasegawa

    JOURNAL OF DENTAL RESEARCH   77 ( 5 )   1305 - 1305   1998.5

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

Research Projects

  • Studying for the role of primary cilium length in tooth development

    Grant number:25K13048

    2025.4 - 2028.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

      More details

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    researchmap

  • Investigation for X-inactivation in palate formation

    Grant number:23K18354

    2023.6 - 2025.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Challenging Research (Exploratory)

    Awarding organization:Japan Society for the Promotion of Science

      More details

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    researchmap

  • 歯の形成におけるDNA損傷/修復のメカニズムの解明

    Grant number:23K09434

    2023.4 - 2026.3

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    川崎 真依子, 大峡 淳, 川崎 勝盛

      More details

    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

    researchmap

  • Study for investigating mechanisms of mandibular development

    Grant number:21K10088

    2021.4 - 2024.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

      More details

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    researchmap

  • Study for investigating mechanisms of mesiodens formation

    Grant number:21K10182

    2021.4 - 2024.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

      More details

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    researchmap

  • 皮膚の付属器官発生における老化活性の機能解明

    Grant number:21H03122

    2021 - 2024

    System name:基盤研究(B)

    Research category:基盤研究(B)

    Awarding organization:日本学術振興会

    大峡 淳(代表者)

      More details

    Grant amount:\17550000 ( Direct Cost: \13500000 、 Indirect Cost:\4050000 )

    歯の再生は、21世紀で成し遂げるべき医療であり、その実現には幹細胞の利用が欠かせない。しかし、幹細胞の歯胚細胞への正しい分化誘導という最も重要な部分の解決が未だなされていない。一般に、器官発生は、胎生幹細胞が少しずつ、より細かな方向へ運命決定をしていくことで達成される。歯は、毛髪などと同じ皮膚の付属器官に属する。全ての皮膚の付属器官が先天的に欠如する遺伝性疾患の存在は、胎生幹細胞がダイレクトに歯胚細胞へ分化するのではなく、皮膚の付属器官への運命決定を行った後に、歯胚細胞への分化を決定している事を示している。胎生幹細胞を、どの付属器官へ分化させるかを決定するメカニズムは、歯の再生における幹細胞の歯胚細胞への分化誘導と同義であるが、未だ明らかでない。胎生期の形成途中の歯胚と毛包に老化シグナルを見出した。さらに老齢マウスの前歯に、異所性の毛髪の形成を認めた。

    researchmap

  • 代生歯堤の形成・維持メカニズムの解明

    2021 - 2022

    System name:挑戦的研究(萌芽)

    大峡 淳(代表者)

      More details

    Authorship:Principal investigator 

    researchmap

  • 顎顔面の発生過程における一次繊毛の機能解明:シグナル経路のクロストークの観点から

    Grant number:20K10092

    2020.4 - 2023.3

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    川崎 真依子, 前田 健康, 大峡 淳, 川崎 勝盛

      More details

    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    一次繊毛 primary ciliaは発生過程に関わる主要なシグナルの制御に関わり、その機能不全は顎顔面形成異常を伴う繊毛病を引き起こす。しかし、顎顔面形成における一次繊毛内での各種シグナル間の相互作用や、複数シグナルによる器官形成制御については未解明のままである。その原因の1つとして複数のシグナルを同時にターゲットとする研究がなされていないことがあげられる。本研究課題は、SHHとWNTシグナル経路を同時に変化させることによって引き起こる形態学的、分子的手法変化を解析することにより、顎顔面発生における一次繊毛の複数シグナル制御の機能を明らかにすることである。今年度は、SHHシグナルを間葉の組織特異的に欠損させ、WNTシグナルを間葉の組織特異的に過剰発現させたマウスの作成とその表現型の解析を行った。SHHシグナルを間葉の組織特異的に欠損させると同時にWNTシグナルを間葉の組織特異的に過剰発現させたマウスは、SHHシグナルを間葉の組織特異的に欠損させたマウスと一部異なる顎顔面領域の異常を示した。顔面の幅径の増加、および歯の形成遅延、眼瞼の形成異常などが挙げられ、これは、WNTシグナルが間葉で過剰発現していることが影響している可能性が示唆された。

    researchmap

  • 舌誘導メカニズムの解明

    Grant number:20K10156

    2020.4 - 2023.3

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    丹原 惇, 大峡 淳, 齊藤 一誠

      More details

    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    舌は、歯科領域で最も癌が発生する部位であり、口腔内で手術によって最も除去される器官とも言える。そのため、舌の再生療法の確立が期待されている。しかし、再生療法確立に必須となる幹細胞の舌への誘導メカニズムが解明されていない。それは、幹細胞誘導に必要な舌の発生メカニズムが明らかになっていないことに起因しており、舌の発生研究の進展が望まれている。器官の発生メカニズムの解明には、目的の器官に異常の存在する実験動物が必須であるものの、舌に異常を持つ遺伝子欠損マウスの報告は多くない。我々は神経堤由来細胞特異的にmicroRNAが欠損したマウスにおいて、舌がほとんど形成されていないことを見出した。本研究は、microRNA欠損マウスの解析から、舌の発生メカニズムを解明することを目的としている。著しく減形成したmicroRNA欠損マウスの舌に筋肉が存在するか、筋細胞マーカーであるMyf5で確認した。野生型マウスでは、Myf5発現細胞と、Myf5非発現細胞が、特定の配列で認められたのに対し、microRNA欠損マウスでは減形成舌の表層にMyf5非発現細胞が、その下方にMyf5発現細胞が認められ、野生型とは異なる配列を示した。

    researchmap

  • A new function of Shh signaling to control epithelial actomyosin contractility during tooth morphogenesis

    Grant number:19K10047

    2019.4 - 2023.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

      More details

    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    researchmap

  • Elucidation of mechanism on congenital abnormality in the maxillofacial region focusing on DNA repair mechanism

    Grant number:19H03849

    2019.4 - 2022.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    MAEDA TAKEYASU

      More details

    Grant amount:\17550000 ( Direct Cost: \13500000 、 Indirect Cost:\4050000 )

    DNA repair is essential process for cell survive. It remains unclear whether DNA repair system is required for development. We generated mice with epithelial conditional deletions of DNA repair-related molecule, Reptin. Reptin cKO showed the arrest of epidermis development. The anomalies of epidermis were found to be caused by DNA damages and subsequent inhibition of cell proliferation due to aberrant activation of p53/p21 signals. We found that oxidative stress damage DNA during normal skin development. DNA damage caused by oxidative stress is repaired by Reptin, which lead to normal skin development.

    researchmap

  • Trail toward development of new treatment for ameloblastoma by intentional cell differentiation

    Grant number:18K19639

    2018.6 - 2021.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Challenging Research (Exploratory)

    Awarding organization:Japan Society for the Promotion of Science

    MAEDA TAKEYASU

      More details

    Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )

    At post-maturation stage in normal tooth development, ameloblasts differentiate into reduced enamel epithelial cells which exhibit low biological activity. These reduced enamel epithelial cells are fallen out during tooth eruption. It is believed that ameloblastoma is caused by tumorigenesis of enamel forming cells. It is possible that ameloblastoma become harmless when ameloblastoma differentiate into reduced enamel epithelial cells. However, the molecular mechanisms inducing reduced enamel epithelial cells are remained unclear. We found that reduced enamel epithelium decreased their biological activity by activating senescence.

    researchmap

  • analysis of the comprehensive molecular mechanism of temporomandibular joint formation

    Grant number:18K09762

    2018.4 - 2021.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    kawasaki katsushige

      More details

    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    Abnormalities of temporomandibular joint are seldom occurred in mice with targeted gene mutation. Therefore, the molecular mechanisms of temporomandibular joint formation remain unclear. We generated mice with mesenchymal deletions of the ciliary protein, Ift88 (Ift88fl/fl;Wnt1Cre), and found aberrant temporomandibular joint. The abnormal temporomandibular joint was found to be caused by aberrant mandibular formation. In Ift88fl/fl;Wnt1Cre mice, downregulation of Hh signaling led to the abnormal mandibular formation, which induced aberrant temporomandibular joint formation.

    researchmap

  • Study of the involvement of epigenetics in cleft palate

    Grant number:17K11954

    2017.4 - 2020.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    ISHIDA Yoko

      More details

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

    Non - syndromic cleft lip with or without cleft palate is one of the most common birth defects in humans. Dicer plays a critical role in the biogenesis of multiple classes of small RNAs. We generated mesenchyme-specific mutations of Dicer using the Cre/loxP-recombination system, and analyzed the role of miRNA in Non - syndromic cleft lip with or without cleft palate.
    The cKO mice showed lower lip cleft. Real-time PCR analysis and in situ hybridization showed that Gli1 mRNA levels decreased significantly in midline in lower lip of cKO mice. We suggested that the lower lip cleft on the Dicer cKO mice associated with Shh pathway.

    researchmap

  • The Mechanism of Palatal Shelve Induction

    Grant number:17K11829

    2017.4 - 2020.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    Kawasaki Maiko

      More details

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

    The role of the primary cilia in maxillary bone development is not fully understood. We generated mice with a mesenchymal conditional deletion of Ift88 and Ofd1 using the Wnt1Cre mice. These proteins involved in the function and formation of primary cilia. It has been shown that Ift88 and Ofd1 KO mice exhibit cleft palate and ectopic bone. We also found ectopic apoptosis in the both mutants maxillary process at an early stage of development. To investigate whether the ectopic apoptosis is related to the both maxillary phenotypes, we generated Ift88 and p53 WKO mutants to reduce apoptosis. These mice showed no excess bone formation, suggesting that the cells evading apoptosis by the presence of Ift88 in wild-type mice limit bone formation in maxillary development. On the other hand, the palatal cleft was retained in this mice, indicating that the excess bone formation or abnormal apoptosis was independent of the cleft palate phenotype in Ift88 mutant mice.

    researchmap

  • Understanding of tooth morphogenesis by studying supernumerary teeth

    Grant number:17K11957

    2017.4 - 2020.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    Saito Yoko

      More details

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    The feature of this study is to analyze human supernumerary teeth by both a combination of the molecular biological technique and the morphological technique.
    First we succeeded to separate only a chamber of the dental pulp and then pick out and analyze it by micro CT as morphological approach.On the other hand, an analysis result by molecular biological technique suggested the possibility that cusp number of a human supernumerary tooth was controlled by different gene in mouse.

    researchmap

  • 分化機構解明による幹細胞の意図的誘導法の開発

    2017 - 2019

    System name:基盤研究(A)

    大峡 淳(代表者)

      More details

    Authorship:Principal investigator  Grant type:Competitive

    researchmap

  • 「生体完結型再生療法」開発への挑戦

    2017 - 2019

    System name:挑戦的研究(開拓)

    大峡 淳(代表者)

      More details

    Authorship:Principal investigator  Grant type:Competitive

    researchmap

  • Analysis of cleft palate mechanism using p53 gene-deficient consomic mice

    Grant number:16H05539

    2016.4 - 2020.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    Takagi Ritsuo

      More details

    Grant amount:\12610000 ( Direct Cost: \9700000 、 Indirect Cost:\2910000 )

    CL / Fr strain mice, which are inbred mice, include mice having both a primary cleft palate and a secondary cleft palate. This time, using CL / Fr strain mice, we analyzed whether there is a difference at the molecular level between primary cleft palate and secondary cleft palate. No change was observed in those signals in the secondary cleft palate region.

    researchmap

  • Elucidation of control mechanism on stem cell differentiation for tooth regeneration

    Grant number:16H05532

    2016.4 - 2019.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    Maeda Takeyasu

      More details

    Grant amount:\17160000 ( Direct Cost: \13200000 、 Indirect Cost:\3960000 )

    Analysis using Ikkβ-K5 mice with overexpression of NF-kB showed a rapture of ameloblasts, ectopic calcification in dental pulp and morphological changes in cervical loop, a niche of stem cells. Furthermore, we found multiple formation of cervical loop, which is due to an increase in cells of stem cell line as confirmed by an elevation of Sox2. Thereafter, ectopic hair growth and enamel formation as well as supernumerary teeth simultaneously ocurred. These findings indicated that an overexpression of NF-kB induces an increase in stem cell niche and a differentiation of individual stem cell into different tissues. In addition, an elevation of NF-kB around stem cell niche suggests that the differentiation and proliferation of stem cells are controlled by these surrounding stem cells.

    researchmap

  • 遺伝子改変マウスを用いた歯誘導メカニズムの網羅的解析

    Grant number:16K11783

    2016.4 - 2018.3

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    原田 史子, 前田 健康, 大峡 淳, 川崎 真依子

      More details

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    本研究の目的は,歯数を決定するメカニズムを解明するため,歯数の制御に関わる歯の発生開始誘導機構を解明することにある.口腔内で歯の欠損と過剰歯が同時に生じるヒト口顔指症候群Ⅰの原因遺伝子Ofd1に着目し,その欠損マウスを作成,解析を行った.
    Cre-LoxPシステムを用い Ofd1を上皮および間葉特異的に欠損させたマウスを作成した. Ofd1上皮特異的欠損マウスでは, 歯数の異常は認められなかった.一方,ホモ型のOfd1間葉特異的欠損マウスでは, 上下顎前歯歯胚が蕾状期で停止し, 上顎臼歯の舌側に過剰歯を認めた.ヘテロ型のOfd1間葉特異的欠損マウスでは, 正常な前歯, 前歯歯胚の停止,上下顎臼歯部の舌側の過剰歯が混在した.これらにより間葉のOfd1が,歯数制御に必須であることが明らかとなった.マイクロアレイやin situ hybridizationを行った結果,ホモ型のOfd1間葉特異的欠損マウス間葉にShhシグナルの著しい低下が確認された.ヘテロ型のOfd1間葉特異的欠損マウスの間葉では, Shhシグナルの活性部位と活性欠如部位がモザイク状に混在した.そこで,Shhシグナル活性に不可欠なSmoの間葉特異的欠損マウスを作成,解析した.その結果,Smo間葉特異的欠損マウスでは, 下顎前歯歯胚は蕾状期で停止し,下顎前歯部に 蕾状期で停止した過剰歯を認め,臼歯部の舌側に過剰歯を認めた.上顎前歯は存在したが,上顎臼歯は存在せず,臼歯部頬側に過剰歯が確認された.
    以上のことから,Ofd1間葉特異的欠損マウスではShhシグナルの低下にも関わらず, ホモ型およびヘテロ型のOfd1間葉特異的欠損マウスと,Smo間葉特異的欠損マウスの歯の表現系は一部のみでしか一致しなかった.このことは,Ofd1による歯数制御はShhシグナルのみではなく複数の分子を介して行われる可能性を示唆する.

    researchmap

  • Elucidation of developmetal mechanism on vermillion by molecular analysis -exploratory study for regeneratuion of vermillion -

    Grant number:16K15773

    2016.4 - 2018.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Challenging Exploratory Research

    Awarding organization:Japan Society for the Promotion of Science

    MAEDA TAKEYASU

      More details

    Grant amount:\3510000 ( Direct Cost: \2700000 、 Indirect Cost:\810000 )

    Vermillion is a human-specific tissue whose reconstruction is esthetically important. However, due to its unique nature, it is impossible to clarify its specificity using animal models. In order to elucidate the tissue specificity of the vermillion, this project examined which molecules are expressed in the adult vermillion. The vermillion expressed long noncoding RNA (lncRNA) and small nuclear RNA (snRNA) more than in the lip mucosa, in addition to PAX gene with the homeobox. Many genes including PPDPF were detected as molecules which never expressed in the mucosa but strongly only in the vermillion. These data suggest that the specificity of the vermillion is controlled by different molecular mechanism from the mucosa.

    researchmap

  • Activation of hedgehog signal pathway induces peripheral nerve regeneration

    Grant number:15H05041

    2015.4 - 2019.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    Seo Kenji

      More details

    Grant amount:\16900000 ( Direct Cost: \13000000 、 Indirect Cost:\3900000 )

    Peripheral nerve injury induces expressions of a morphogen Sonic Hedgehog (Shh) and its transcriptional regulator Gli1 in the lesion. Blockade of Shh signaling pathway after peripheral nerve injury leads to abnormal axon growth in random directions, resulting in a disturbance of axonal regeneration. This also increases the number of immature Schwann cell in the medial site of the lesion, but it decreases that of macrophage in the distal site. During the regeneration period, the expression of Shh in the lesion elevates immediately after the injury and attenuates later. In contrast, another homologues Hedgehog family Desert hedgehog (Dhh) exhibits the reverse process. This implicates that nerve injury induces switching the expression of Shh to Dhh and these factors contribute to peripheral nerve regeneration.

    researchmap

  • Analysis of the cleft lip/palate development mechanism using the p53 knockout mouse with Artificial insemination frozen embryos

    Grant number:15K11237

    2015.4 - 2018.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    Kodama Yasumitsu

      More details

    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

    We regarded p53 gene known as tumor suppressor gene as "a guardian of the teratogenesis". It was intended to search effect of p53 on cleft lip and palate formation in the CL/Fr mouse (cleft lip and palate sensitive strain). We analyzed to use the p53 knockout CL/Fr mouse artificial insemination frozen embryos.
    Cleft lip in the CL/Frp53(-/-) mouse was suggested to be caused by specific malformation without the report so far. The cleft palate in the p53(-/-) mouse was almost the clinical condition of so-called complete cleft palate, and the palatine process was found. The size of the palatine process was very small, and most of the growth to a horizontal direction was proved not to occur.

    researchmap

  • Tissue engineering of Temporomandibular joint organoids

    Grant number:15K15674

    2015.4 - 2017.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Challenging Exploratory Research

    Awarding organization:Japan Society for the Promotion of Science

    NOZAWA-INOUE KAYOKO

      More details

    Grant amount:\3640000 ( Direct Cost: \2800000 、 Indirect Cost:\840000 )

    Understanding the molecular mechanisms in regulating the temporomandibular joint (TMJ) development is essential for generating TMJ tissue engineering. However, even the phase and place of TMJ initiation that are necessary to analysis of molecular mechanisms are not established yet. We could define the suitable embryos in this study which were selected by their body weight, not by the fatal date. Runx2 and Sox9 were expressed in the presumptive region of the TMJ. Furthermore, 3D analysis for these gene expressions indicated that the condylar anlage was connected with the periosteum of the developing mandibular ramus. We also used organ culture techniques, but morphologically and genetically normal TMJ was not obtained by the modification of the ordinary methods. These results suggested that the completely new method of organ culture was required for the future study of TMJ tissue engineering.

    researchmap

  • Reprogramming of palatal mucosa into tooth-forming epithelium

    Grant number:26670848

    2014.4 - 2016.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Challenging Exploratory Research

    Awarding organization:Japan Society for the Promotion of Science

    MAEDA TAKEYASU, OHAZAMA Atsushi, INOUE Kayoko

      More details

    Grant amount:\3640000 ( Direct Cost: \2800000 、 Indirect Cost:\840000 )

    We investigated the molecular differences between tooth and palatal rugae development in wild-type mice. In common with tooth germ cells, mesenchymal cells of the developing palatal rugae were found to be derived from the neural crest. Many tooth-related genes such as Bmp4 and Lrp4 showed expression in the palatal rugae, and signaling pathways including Bmp and Shh (which are known to regulate tooth development) were also activated during palatal rugae formation. However, expression of several tooth-related molecules including Msx1 could not be detected within the developing palatal rugae. Transgenic mice with altered expression of the molecules found in the developing rugae exhibited highly disorganized palatal rugae.

    researchmap

  • 幹細胞の分化制御機構の解明の基盤研究 〜NF-kBからの解析〜

    2014 - 2016

    System name:基盤研究(B)

    大峡 淳(代表者)

      More details

    Authorship:Principal investigator  Grant type:Competitive

    researchmap

  • 歯の発生における時間軸変更への挑戦

    2014 - 2015

    System name:挑戦的萌芽研究

    大峡 淳(代表者)

      More details

    Authorship:Principal investigator  Grant type:Competitive

    researchmap

  • Roles of new ion channel involved in the regeneration/development of the periodontal Ruffini endings

    Grant number:23390418

    2011.4 - 2014.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    MAEDA Takeyasu, IZUMI Kenji, INOUE Kayoko, KAWANO Yoshiro, OHZAMA Atsushi, SUZUKI Akiko, HARADA Fumiko, YOSHII Tomoko, IHYO Chika

      More details

    Grant amount:\18850000 ( Direct Cost: \14500000 、 Indirect Cost:\4350000 )

    This study examined the regeneration process of the periodontal Ruffini ending, an essential mecahnoreceptor, based on the changes in the expression pattern of acid-sensing ion channel-3 (ASIC3) at the periodontal ligament and trigeminal ganglion. The findings obtained from the nerve injury model (resection of the inferior alveoar nerve at one side) indicate that this molecule is not direcly involved in the axonal regeneration of the periodontal Ruffini endings but rather in neuron-glial interactions such as control of neuronal activity in the trigeminal ganglion.

    researchmap

  • The mechanisms of NF-kB in ectodermal appendages

    Grant number:16592080

    2004 - 2005

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    OHAZAMA Atsushi

      More details

    Grant amount:\3600000 ( Direct Cost: \3600000 )

    The NF-κB pathway plays a major role in many physiological and pathological processes. It has been shown that NF-κB and Ikkα are involved in tooth development. Evagination was observed in presumptive incisor region of Ikkα mutant mice. The downregulation of Notch have been found in Ikkα mutant incisor tooth. In wild-type, the expression of Notch were observed in the stellate reticulum (SR) of tooth bud epithelium while they were absent from the basal epithelial cells. The downregulation of Notch expression in Ikkα mutant incisor tooth germ reveal the possibility that the failure of the SR formation led to the loss of Notch expression in Ikkα mutant incisor. That prompt us to investigate whether the SR are presented in Ikkα mutant incisor or not. The SR marker, CD44 and SLUG expression were detected in the incisor epithelium of Ikkα mutant mice. The SR have been reported to play a critical role in enamel formation through Msx2 signalling. Msx2 and normal enamel deposition were detected in Ikkα mutant incisor tooth. These suggested that the SR were existed in Ikkα mutant incisor tooth. On the other hand, there have been no reports that describe the relationship between Ikkα and Notch. Ikkα mutant mice also showed cleft palate that were caused by unelevated palatal shelves and fusion between tongue and palatal shelves. Same phenotypes also have been found in Notch ligand, Jagged2 mutant mice. The data should be another evidence of the relationship between Ikkα and Notch during development. Ikkα/Notch are essential as kinase independent pathway in tooth development.

    researchmap

  • A study of the role of chemokine in regulation of human osteoclast differentiation

    Grant number:16592077

    2004 - 2005

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    OKAMATSU Yoshimasa, KOBAYASHI Makoto, MIYAZAWA Yasushi, OHAZAMA Atsushi, TAKIGUCHI Takashi

      More details

    Grant amount:\3600000 ( Direct Cost: \3600000 )

    Osteoclasts (OC) are bone resorbing multinucleated giant cells that are derived from hemopoietic precursors of the monocyte-macrophage lineage. Osteoclastic bone resorption consists of multiple steps, including the differentiation of osteoclast precursors, the fusion of mononuclear cells to form mature OC, the activation of OC to resorb bone and the survival of activated. RANKL (Receptor Activator of NF-κB Ligand) is member of the TNF family and is one of key molecules that regulates both osteoclastogenesis and bone resorption. RANKL expression by osteoblasts as well as by activated T cells has been shown to regulate these processes. However, the participation of additional factors induced by RANKL stimulation is less well characterized.
    Chemokines play an important role in immune and inflammatory responses by inducing migration and adhesion of leukocytes, and have also been reported to participate in the regulation of osteoclast (OC) differentiation from hematopoietic precursor cells of the monocyte-macrophage lineage. However, the effect of these chemokines as coupling factor between osteoclast and osteoblasts remain unclear. In this study, we can get these results until now.
    1)RANKL-induced mature osteoclast from mouse bone marrow up-regulated the expression of MIP (Macrophage inflammatory protein)-1 gamma gene and CCR1 gene.
    2)The signal tranceduction of MIP-1 gamma indicated via NF-kB.
    3)RANKL-induced mature osteoclast from mouse bone marrow also up-regulated the expression of integrin beta7 gene and MIP-1 gamma related to expression of integrin beta7 gene in osteoclasts.
    4)RANKL and M-CSF stimulated-CD14 positive human monocyte differentiated into mature osteoclasts, and these cells released MIP-1 alpha.
    5)IL-1-stimulated osteoblasts from mouse calvaria produced RANTES and MIP-1 alpha.
    These results suggest that many chemokines related osteoclast differentiation.

    researchmap

  • Determination of the specific phenotype markers of ex vivo expanded human peridontal ligament cells

    Grant number:16592078

    2004 - 2005

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    KOBAYASHI Makoto, MURAKAMI Shinya, MIYAZAWA Yasushi, OHAZAMA Atsushi, OKAMATSU Yoshimasa, TAKIGUCHI Takashi

      More details

    Grant amount:\3600000 ( Direct Cost: \3600000 )

    The transplantation of cultured human periodontal ligament (HPL) cells with scaffolds into periodontal defects appears to be a powerful strategy to promote periodontal regeneration. To clarify the specific phenotype markers of the cultured HPL cells, we investigated here the frequency of HPL cells expressed marker antigens of various cell types and the frequency of three types of mesenchymal progenitor cells existed within HPL cells, compared with human mesenchymal stem cells (HMSCs).
    Frequency of HPL cells expressed markers of various cell types
    In HPL cells, Ratio of cells expressed MSC markers (CD105,CD44,CD29) or integrins (CD49c, CD49e) were high, and those of cells expressed a hematopoietic stem cell marker (CD117), hemotopoietic cell markers (CD34,CD45) or a neuronal stem cell marker (AC133) were low levels.
    Frequency of three types of mesenchymal progenitor cells existed within cultured HPL cells
    The expression profile of MSC markers ; CD45 negative (CD45^-),CD90 positive (CD90^+),CD105^+,CD 146^+ or STRO-1^+, in HPL cells was similar to that in HMSCs, suggesting that many constituent cells of HPL cell cultures possess MSC phenotype. However, the potential of multilineage differentiation in HPL cells was different from that in HMSCs. HMSCs highly differentiated into three mesenchymal cell types, osteoblasts, adopocytes and chondrocytes under each lineage-specific culture condition. Also in HPL cells, the increase of ALP^+ cell number, mineralized nodule formation and expression of bone-specific genes was detected. Whereas, the number of adpocytes induced in HPL cells cultures was a few, and the development of cartilage containing type II collagen were not detected in HPL cell pellet cultures. These results suggest that the cultured HPL cells contain many osteoblast lineage cells and small number of adipocyte precursors, while chondrocyte precursors might exist at slight levels or not within the cells. Further, ALP^- cell fraction isolated from HPL cells contained relatively more adipocyte precursors.

    researchmap

  • 歯根膜の組織特異性に関する基礎的研究

    Grant number:09771646

    1997 - 1998

    System name:科学研究費助成事業

    Research category:奨励研究(A)

    Awarding organization:日本学術振興会

    大峡 淳

      More details

    Grant amount:\1900000 ( Direct Cost: \1900000 )

    歯根膜の組織特異性の有無を、外骨膜との比較により検索した。
    外骨膜、歯根膜、骨組織それぞれから得た細胞間でALPase活性やPTHによるcAMP産生などに違いは認められなかった。
    歯根膜を除去し、外骨膜をラッピングした歯根の顎骨肉への移植では、移植歯根表面への新生セメント質の形成は認められなかったものの、移舶歯根の骨性癒着もあまり観察されなかった。また外骨膜から得た細胞を培養した象牙質片の顎骨内への移植でも、同様の結果が認められた。
    また外骨膜および歯根膜それぞれを培養した象牙質片の移植後に発現する蛋白の検索においても、両者間に違いは認められなかった。
    今回の結果では、外骨膜には多数の骨芽細胞様細胞が含まれるものの、いずれの外骨膜応用群でも新生セメント貿は観察されなかった。一方、そのような多数の骨芽細胞様細胞が含まれるにもかかわらず、骨性癒着はほとんど観察されなかった。以上のことから、歯根膜という非石灰化組織の維持は歯根膜以外の組織でも可能であるのに対し、セメント質形成は歯根膜にのみ存在する特異的能力である可能性が高い事が示唆された。今後例数を増やし、さらに検索していく予定である。
    またこの石灰化抑制を検討する目的で、mineralized bone nodule形成能を利用した検索を、現在行っている。

    researchmap

  • 歯槽骨再生に及ぼす歯根膜の関与に関する基礎的研究

    Grant number:08771732

    1996

    System name:科学研究費助成事業

    Research category:奨励研究(A)

    Awarding organization:日本学術振興会

    大峡 淳

      More details

    Grant amount:\900000 ( Direct Cost: \900000 )

    歯周病により破壊された骨欠損にGTR法を応用した場合、新生セメント質を伴う結合組織性付着の獲得はみられるものの、骨の再生は非常に少ないことが分かってきた。これに対して、インプラント周囲の骨欠損や歯槽堤形成術においてGBR法を応用した場合には、十分な骨再生がみられることが報告されている。両者間でこのような骨再生の違いかなる理由により生じるかについては、骨が再生するスペースや、骨欠損形態が関与するのではないかという報告があるがいまだ明確な答は得られていない。そこで我々は、歯周組織再生過程で歯根膜の存在の有無が歯槽骨の再生に影響を与えているのではないかという仮説を立て、その仮説を明確にすることを目的とした実験を考案して検討を進めてきた。
    その結果、犬を用いて歯根膜存在群と歯根膜除去群とのそれぞれの歯槽骨再生に対する影響を観察すると臨床所見並びにエックス線所見においては、一部の標本で歯根膜除去群が歯根膜存在群に比べて骨形成量の多い傾向が認められたが、ほとんどの標本では両群間で差は認められなかった。組織学的所見においては、いずれの犬においても歯根膜存在群では新生骨骨頂まで歯根膜の再生が認められたのに対し、歯根膜除去群では著名な歯根吸収ならびに歯根と歯槽骨とのアンキロ-シスが認められた。両群ともに分割歯根の歯冠側端まで新生骨の形成が認められ、両群間で差は認められなかった。また、免疫組織化学的検索においても両群間で差は認められなかった。
    歯根膜存在群、歯根膜除去群から新生肉芽組織を採取し、それらの生化学的検索およびDiffusion Chamberを用いたそれらの石灰化形成能の検索については、現在組織切片等の作成中である。
    現在までの結果より考察すると、歯根膜の存在は歯槽骨再生にあまり影響しない可能性のある事が考えられるが、進行中の実験も含め今後更なる検討を加える予定である。

    researchmap

  • 歯根膜細胞の有無が歯槽骨再生に及ぼす影響についての基礎的研究

    Grant number:07771783

    1995

    System name:科学研究費助成事業

    Research category:奨励研究(A)

    Awarding organization:日本学術振興会

    大峡 淳

      More details

    Grant amount:\1000000 ( Direct Cost: \1000000 )

    歯周病の治癒に対する理想的な治癒形態は、歯周組織の再生であり、歯根膜組織由来及び歯槽骨由来の細胞が重要な役割を演じている。そこで近年それらを選択的に誘導するGTR法が考案されたが、骨の再生は非常に少ないことが明らかとなり、それに比べインプラントにおいてGTR法を応用した場合では十分な骨の再生が得られることが報告されている。これらの違いに対する明確な答は得られていないが、歯根膜由来細胞が骨芽細胞様細胞であるとする報告や歯根膜組織が正常な場合石灰化抑制因子を産生しているとの報告などがある。そこで今回、この歯根膜の存在の有無が、歯周組織再生特に歯槽骨の再生にどのような影響を及ぼすか、またその要因は何かを明確にすることを目的として実験を行った。
    成犬の前臼歯を抜歯し治癒させた後、他の前臼歯を歯根膜有り群、歯根膜無し群に分け、その抜歯窩治癒部に移植し、その頬側に骨欠損を作製した。その結果、歯根膜有り群と無し群との間で、骨欠損内でのセメント質及び歯槽骨の再生量に有為な差は認められなかった。その考察として、移植歯であってもsubmergeでGTR法を行った場合、今回採用した程度の大きさの骨欠損では両群とも良好に治癒し、歯根膜の有無の骨形成への影響が確認できなかったものと考えられた。そのため大幅に骨欠損を拡大し同様の実験を行ったところ両群間に差が認められたので、現在例数を増やしその再現性を確認している。それに並行して、その両群の骨欠損内に形成された新生肉芽組織の生化学的検索を行ったが、アルカリフォスファターゼ活性やtypeIコラーゲン量に若干の差が認められ、現在その再現性の確認を行っている。またその新生肉芽組織のラット膜腔内への移植を開始した。今後、両群からの新生肉芽組織の器官培養における培養上清中のPGE_2やMMP-1などの測定や、out growthさせた細胞のmineralized bone nodule形成能を検討していく予定である。

    researchmap

▶ display all

 

Teaching Experience

  • 口腔保健福祉学研究論I

    2024
    Institution name:新潟大学

  • 顎口腔解剖学演習IA

    2021
    Institution name:新潟大学

  • 基礎歯学コースワーク(ベーシック形態解析コースII)

    2021
    Institution name:新潟大学

  • 顎口腔解剖学演習IB

    2021
    Institution name:新潟大学

  • 顎口腔解剖学演習IIB

    2021
    Institution name:新潟大学

  • 顎口腔解剖学演習IIA

    2021
    Institution name:新潟大学

  • 基礎歯学コースワーク(ベーシック形態解析コースI)

    2021
    Institution name:新潟大学

  • ネットワーク型先端歯学研究

    2021
    Institution name:新潟大学

  • 基礎歯学コースワーク(ベーシック形態解析コースⅡ)

    2020
    Institution name:新潟大学

  • 基礎歯学コースワーク(ベーシック形態解析コースⅠ)

    2017
    -
    2018
    Institution name:新潟大学

  • 顎口腔解剖学演習ⅠA

    2017
    -
    2018
    Institution name:新潟大学

  • 顎口腔解剖学演習ⅠB

    2017
    Institution name:新潟大学

  • 人体発生学

    2016
    Institution name:新潟大学

  • 統合科目Ⅰ

    2016
    -
    2019
    Institution name:新潟大学

  • 人体のしくみ

    2015
    Institution name:新潟大学

  • 早期臨床実習Ⅱ

    2015
    -
    2020
    Institution name:新潟大学

  • 組織学総論

    2014
    Institution name:新潟大学

  • 組織学各論

    2014
    Institution name:新潟大学

  • 口腔組織発生学

    2014
    Institution name:新潟大学

  • 基礎科学演習

    2014
    -
    2016
    Institution name:新潟大学

▶ display all