Updated on 2026/09/24
農学 ( 2003.3 東北大学 )
Life Science / Genome biology / トランスポゾン
Environmental Science/Agriculture Science / Science in plant genetics and breeding / 生殖
新潟大学 自然科学系 (農学部) 准教授
2018.10
新潟大学 自然科学系 (農学部) 助教
2014.4 - 2018.9
日本学術振興会特別研究員 (PD) 独立行政法人 農業生物資源研究所
2006.4 - 2008.3
日本学術振興会海外特別研究員 (デンマーク、オーフス大学)
2004.4 - 2006.3
Niigata University Faculty of Agriculture Department of Agriculture Associate Professor
2018.10
Niigata University Faculty of Agriculture Department of Agriculture Assistant Professor
2017.4 - 2018.9
Niigata University Abolition organization Agrobiology Assistant Professor
2014.4 - 2017.3
東北大学大学院 農学研究科 応用生命科学専攻
1998.4 - 2003.3
東北大学 農学部
1994.4 - 1998.3
Differential regulation of heterochromatin and euchromatin by GmDDM1 during seed development ensures seedling viability in soybean. Reviewed International journal
Ahsen Gers, Kana Shiraishi, Kaoru Tonosaki, Satoru Okamoto, Akito Kaga, Ryota Kuroda, Jun-Ichi Matsuoka, Atsushi Toyoda, Taiji Kawakatsu, Chiho Maruko, Kazuki Takahashi, Keiichi Okazaki, Moeko Okada, Eigo Fukai
<i>The Plant Journal</i> 126 ( 4 ) e70921 2026.5
Genetic control of seed iron and zinc concentration in Rwandan common bean population revealed by the Genome Wide Association Study (GWAS). Reviewed
Floride Mukamuhirwa, Kenta Shirasawa, Ken Naito, Edouard Rurangwa, Viateur Ndayizeye, Alphonse Nyombayire, Jean Pierre Muhire, Mahalingam Govindaraj, Norikuni Ohtake, Keiichi Okazaki, Moeko Okada, Eigo Fukai
<i>Breeding Science</i> 75 ( 3 ) 187 - 199 2025.6
Propagation path of a flowering cherry (<i>Cerasus</i> x <i>yedoensis</i>) cultivar 'Somei-Yoshino' traced by somatic mutations Reviewed
Kenta Shirasawa, Tomoya Esumi, Akihiro Itai, Katsunori Hatakeyama, Tadashi Takashina, Takuji Yakuwa, Katsuhiko Sumitomo, Takeshi Kurokura, Eigo Fukai, Keiichi Sato, Takehiko Shimada, Katsuhiro Shiratake, Munetaka Hosokawa, Yuki Monden, Makoto Kusaba, Hidetoshi Ikegami, Sachiko Isobe
<i>DNA Research</i> 31 ( 5 ) dsae025 2024.9
Quantitative trait locus (QTL) analysis and fine-mapping for <i>Fusarium oxysporum</i> disease resistance in <i>Raphanus sativus</i> using GRAS-Di technology. Reviewed
Chukwunonso Sylvanus Austin Ezeah, Juichi Shimazu, Takahiro Kawanabe, Motoki Shimizu, Shinichi Kawashima, Makoto Kaji, Charles Onyemaechi Ezinma, Md Nuruzzaman, Nami Minato, Eigo Fukai, Keiichi Okazaki
<i>Breeding Science</i> 73 ( 5 ) 421 - 434 2023.12
Comparative anatomical and hormonal analyses between kohlrabi and broccoli seedlings: Relevance to kohlrabi stem tuber initiation Reviewed
Md. Nuruzzaman, Mikiko Kojima, Masato Sato, Yumiko Takebayashi, Mozammel Hoque, Satoru Okamoto, Daniel J. Shea, Ryo Fujimoto, Eigo Fukai, Hitoshi Sakakibara, Keiichi Okazaki
<i>Scientia Horticulturae</i> 316 112002 - 112002 2023.6
Comparative transcriptome analysis during tuberous stem formation in Kohlrabi (<i>B. oleracea</i> var. <i>gongylodes</i>) at early growth periods (seedling stages). Reviewed International journal
Md Nuruzzaman, Masato Sato, Satoru Okamoto, Hoque Mozammel, Daniel J Shea, Ryo Fujimoto, Motoki Shimizu, Eigo Fukai, Keiichi Okazaki
<i>Physiologia Plantarum</i> 174 ( 5 ) e13770 2022.8
Widespread and transgenerational retrotransposon activation in inter- and intraspecies recombinant inbred populations of <i>Lotus japonicus</i>. Reviewed International journal
Eigo Fukai, Manabu Yoshikawa, Niraj Shah, Niels Sandal, Akio Miyao, Seijiro Ono, Hideki Hirakawa, Turgut Yigit Akyol, Yosuke Umehara, Ken-Ichi Nonomura, Jens Stougaard, Hirohiko Hirochika, Makoto Hayashi, Shusei Sato, Stig Uggerhøj Andersen, Keiichi Okazaki
<i>The Plant Journal</i> 111 ( 5 ) 1397 - 1410 2022.7
Identification of a Male Sterile Candidate Gene in <i>Lilium</i> x <i>formolongi</i> and Transfer of the Gene to Easter Lily (<i>L. longiflorum</i>) via Hybridization. Reviewed International journal
Takahiro Moriyama, Daniel John Shea, Naoto Yokoi, Seiro Imakiire, Takaaki Saito, Hikaru Ohshima, Hina Saito, Satoru Okamoto, Eigo Fukai, Keiichi Okazaki
<i>Frontiers in Plant Science</i> 13 914671 - 914671 2022.6
A chromosome-level genome sequence of <i>Chrysanthemum seticuspe</i>, a model species for hexaploid cultivated chrysanthemum. Reviewed International journal
Michiharu Nakano, Hideki Hirakawa, Eigo Fukai, Atsushi Toyoda, Rei Kajitani, Yohei Minakuchi, Takehiko Itoh, Yohei Higuchi, Toshiaki Kozuka, Hidemasa Bono, Kenta Shirasawa, Ippei Shiraiwa, Katsuhiko Sumitomo, Tamotsu Hisamatsu, Michio Shibata, Sachiko Isobe, Kenji Taniguchi, Makoto Kusaba
<i>Communications Biology</i> 4 ( 1 ) 1167 - 1167 2021.10
Extreme genetic signatures of local adaptation during <i>Lotus japonicus</i> colonization of Japan. Reviewed International journal
Niraj Shah, Tomomi Wakabayashi, Yasuko Kawamura, Cathrine Kiel Skovbjerg, Ming-Zhuo Wang, Yusdar Mustamin, Yoshiko Isomura, Vikas Gupta, Haojie Jin, Terry Mun, Niels Sandal, Fuyuki Azuma, Eigo Fukai, Ümit Seren, Shohei Kusakabe, Yuki Kikuchi, Shogo Nitanda, Takashi Kumaki, Masatsugu Hashiguchi, Hidenori Tanaka, Atsushi Hayashi, Mads Sønderkær, Kaare Lehmann Nielsen, Korbinian Schneeberger, Bjarni Vilhjalmsson, Ryo Akashi, Jens Stougaard, Shusei Sato, Mikkel Heide Schierup, Stig Uggerhøj Andersen
<i>Nature Communications</i> 11 ( 1 ) 253 - 253 2020.1
Allele specific DNA marker for fusarium resistance gene FocBo1 in Brassica oleracea Reviewed
Maho Sato, Motoki Shimizu, Daniel J. Shea, Mozammel Hoque, Takahiro Kawanabe, Naomi Miyaji, FUJIMOTO RYO, Eigo Fukai, Keiichi Okazaki
<i>Breeding Science</i> 69 ( 2 ) 308 - 315 2019.7
An LTR retrotransposon insertion was the cause of world’s first low erucic acid <i>Brassica rapa</i> oilseed cultivar Reviewed
Eigo Fukai, Md. Masud Karim, Daniel J. Shea, Nazmoon Naher Tonu, Kevin C. Falk, Taketo Funaki, Keiichi Okazaki
<i>Molecular Breeding</i> 39 ( 1 ) 2019.1
The production and characterization of a <i>BoFLC2</i> introgressed <i>Brassica rapa</i> by repeated backcrossing to an F<sub>1</sub>. Reviewed
Shea DJ, Tomaru Y, Itabashi E, Nakamura Y, Miyazaki T, Kakizaki T, Naher TN, Shimizu M, Fujimoto R, Fukai E, Okazaki K
<i>Breeding Science</i> 68 ( 3 ) 316 - 325 2018.6
EAT1 transcription factor, a non-cell-autonomous regulator of pollen production, activates meiotic small RNA biogenesis in rice anther tapetum Reviewed
Seijiro Ono, Hua Liu, Katsutoshi Tsuda, Eigo Fukai, Keisuke Tanaka, Takuji Sasaki, Ken-Ichi Nonomura
<i>PLOS Genetics</i> 14 ( 2 ) e1007238 2018.2
IntroMap: a signal analysis based method for the detection of genomic introgressions Reviewed
Daniel J. Shea, Motoki Shimizu, Namiko Nishida, Eigo Fukai, Takashi Abe, Ryo Fujimoto, Keiichi Okazaki
<i>BMC Genetics</i> 18 2017.12
QTL mapping for tuberous stem formation of kohlrabi (<i>Brassica oleracea</i> var. <i>gongylodes</i> L.) Reviewed
Mozammel Hoque, Daniel J. Shea, Mitsuru Asada, Md. Asad-ud-Doullah, Motoki Shimizu, Ryo Fujimoto, Eigo Fukai, Keiichi Okazaki
<i>Molecular Breeding</i> 37 ( 9 ) 2017.9
The <i>LORE1</i> insertion mutant resource Reviewed
Anna Malolepszy, Terry Mun, Niels Sandal, Vikas Gupta, Manu Dubin, Dorian Urbanski, Niraj Shah, Asger Bachmann, Eigo Fukai, Hideki Hirakawa, Satoshi Tabata, Marcin Nadzieja, Katharina Markmann, Junyi Su, Yosuke Umehara, Takashi Soyano, Akira Miyahara, Shusei Sato, Makoto Hayashi, Jens Stougaard, Stig U. Andersen
<i>The Plant Journal</i> 88 ( 2 ) 306 - 317 2016.10
Marker-assisted selection of low erucic acid quantity in short duration <i>Brassica rapa</i> Reviewed
Md. Masud Karim, Nazmoon Naher Tonu, Mohammad Shakhawat Hossain, Taketo Funaki, M. Bahadur Meah, Delwar M. Hossain, M. Asad ud-Doullah, Eigo Fukai, Keiichi Okazaki
<i>Euphytica</i> 208 ( 3 ) 535 - 544 2016.4
Changes in the proteome of xylem sap in <i>Brassica oleracea</i> in response to <i>Fusarium oxysporum</i> stress Reviewed
Zijing Pu, Yoko Ino, Yayoi Kimura, Asumi Tago, Motoki Shimizu, Satoshi Natsume, Yoshitaka Sano, Ryo Fujimoto, Kentaro Kaneko, Daniel J. Shea, Eigo Fukai, Shin-Ichi Fuji, Hisashi Hirano, Keiichi Okazaki
<i>Frontiers in Plant Science</i> 7 2016.2
Loss of function mutations in the rice chromomethylase OsCMT3a cause a burst of transposition Reviewed
Chaoyang Cheng, Yoshiaki Tarutani, Akio Miyao, Tasuku Ito, Muneo Yamazaki, Hiroaki Sakai, Eigo Fukai, Hirohiko Hirochika
<i>The Plant Journal</i> 83 ( 6 ) 1069 - 1081 2015.9
Map-based cloning of a candidate gene conferring Fusarium yellows resistance in <i>Brassica oleracea</i> Reviewed
Motoki Shimizu, Zi-jing Pu, Takahiro Kawanabe, Hiroyasu Kitashiba, Satoru Matsumoto, Yusuke Ebe, Monari Sano, Taketo Funaki, Eigo Fukai, Ryo Fujimoto, Keiichi Okazaki
<i>Theoretical and Applied Genetics</i> 128 ( 1 ) 119 - 130 2015.1
The SNARE protein SYP71 expressed in vascular tissues Is involved in symbiotic nitrogen fixation in <i>Lotus japonicus</i> nodules Reviewed
Tsuneo Hakoyama, Ryo Oi, Kazuya Hazuma, Eri Suga, Yuka Adachi, Mayumi Kobayashi, Rie Akai, Shusei Sato, Eigo Fukai, Satoshi Tabata, Satoshi Shibata, Guo-Jiang Wu, Yoshihiro Hase, Atsushi Tanaka, Masayoshi Kawaguchi, Hiroshi Kouchi, Yosuke Umehara, Norio Suganuma
<i>Plant Physiology</i> 160 ( 2 ) 897 - 905 2012.10
Establishment of a <i>Lotus japonicus</i> gene tagging population using the exon-targeting endogenous retrotransposon <i>LORE1</i> Reviewed
Eigo Fukai, Takashi Soyano, Yosuke Umehara, Shinobu Nakayama, Hideki Hirakawa, Satoshi Tabata, Shusei Sato, Makoto Hayashi
<i>The Plant Journal</i> 69 ( 4 ) 720 - 730 2012.2
Sequence analysis of the genome of an oil-bearing tree, <i>Jatropha curcas</i> L. Reviewed
Sato S, Hirakawa H, Isobe S, Fukai E, Watanabe A, Kato M, Kawashima K, Minami C, Muraki A, Nakazaki N, Takahashi C, Nakayama S, Kishida Y, Kohara M, Yamada M, Tsuruoka H, Sasamoto S, Tabata S, Aizu T, Toyoda A, Shin-I T, Minakuchi Y, Kohara Y, Fujiyama A, Tsuchimoto S, Kajiyama S, Makigano E, Ohmido N, Shibagaki N, Cartagena JA, Wada N, Kohinata T, Atefeh A, Yuasa S, Matsunaga S, Fukui K
<i>DNA Research</i> 18 ( 1 ) 65 - 76 2010.12
Derepression of the plant chromovirus <i>LORE1</i> induces germline transposition in regenerated plants Reviewed
Eigo Fukai, Yosuke Umehara, Shusei Sato, Makoto Endo, Hiroshi Kouchi, Makoto Hayashi, Jens Stougaard, Hirohiko Hirochika
<i>PLOS Genetics</i> 6 ( 3 ) 2010.3
Rearrangement of actin cytoskeleton mediates invasion of <i>Lotus japonicus</i> roots by <i>Mesorhizobium loti</i> Reviewed
Keisuke Yokota, Eigo Fukai, Lene H. Madsen, Anna Jurkiewicz, Paloma Rueda, Simona Radutoiu, Mark Held, Md Shakhawat Hossain, Krzysztof Szczyglowski, Giulia Morieri, Giles E. D. Oldroyd, J. Allan Downie, Mette W. Nielsen, Anna Maria Rusek, Shusei Sato, Satoshi Tabata, Euan K. James, Hiroshi Oyaizu, Niels Sandal, Jens Stougaard
<i>The Plant Cell</i> 21 ( 1 ) 267 - 284 2009.1
A retrotransposon-inserted <i>VvmybA1a</i> allele has been spread among cultivars of <i>Vitis vinifera</i> but not North American or East Asian <i>Vitis</i> species Reviewed
N. MITANI, A. AZUMA, E. FUKAI, H. HIROCHIKA, S. KOBAYASH
<i>Vitis</i> 48 ( 1 ) 55 - 56 2009.1
Transposition of a 600 thousand-year-old LTR retrotransposon in the model legume <i>Lotus japonicus</i> Reviewed
Eigo Fukai, Alicja Dorota Dobrowolska, Lene Heegaard Madsen, Esben Bjorn Madsen, Yosuke Umehara, Hiroshi Kouchi, Hirohiko Hirochika, Jens Stougaard
<i>Plant Molecular Biology</i> 68 ( 6 ) 653 - 663 2008.12
LysM domains mediate lipochitin-oligosaccharide recognition and <i>Nfr</i> genes extend the symbiotic host range Reviewed
Simona Radutoiu, Lene H. Madsen, Esben B. Madsen, Anna Jurkiewicz, Eigo Fukai, Esben M. H. Quistgaard, Anita S. Albrektsen, Euan K. James, Soren Thirup, Jens Stougaard
<i>EMBO Journal</i> 26 ( 17 ) 3923 - 3935 2007.9
Suppression of gene expression of a recessive <i>SP11/SCR</i> allele by an untranscribed <i>SP11/SCR</i> allele in <i>Brassica</i> self-incompatibility Reviewed
Ryo Fujimoto, Tetsu Sugimura, Eigo Fukai, Takeshi Nishio
<i>Plant Molecular Biology</i> 61 ( 4-5 ) 577 - 587 2006.7
Comparison of the genome structure of the self-incompatibility (<i>S</i>) locus in interspecific pairs of <i>S</i> haplotypes Reviewed
Ryo Fujimoto, Keiichi Okazaki, Eigo Fukai, Makoto Kusaba, Takeshi Nishio
<i>Genetics</i> 173 ( 2 ) 1157 - 1167 2006.6
LORE1, an active low-copy-number TY3-<i>gypsy</i> retrotransposon family in the model legume <i>Lotus japonicus</i> Reviewed
LH Madsen, E Fukai, S Radutoiu, CK Yost, N Sandal, L Schauser, J Stougaard
<i>The Plant Journal</i> 44 ( 3 ) 372 - 381 2005.11
Genomic organization of the <i>S</i> core region and the <i>S</i> flanking regions of a class-II <i>S</i> haplotype in <i>Brassica rapa</i>. Reviewed
Fukai E, Fujimoto R, Nishio T
<i>Molecular Genetics and Genomics</i> 269 361 - 369 2003.4
Molecular genetic analysis of the candidate gene for <i>MOD</i>, a locus required for self-incompatibility in <i>Brassica rapa</i>. Reviewed
Fukai E, Nishio T, Nasrallah ME
<i>Molecular Genetics and Genomics</i> 265 519 - 526 2001.5
Somatic transposition of transposable elements and its significance in horticultural breeding Reviewed
Eigo Fukai, Ahsen Gers
<i>The Horticulture Journal</i> 2026.9
The role of <i>FLOWERING LOCUS C</i> in vernalization of <i>Brassica</i>: The importance of vernalization research in the face of climate change Reviewed
Daniel J. Shea, Etsuko Itabashi, Satoko Takada, Eigo Fukai, Tomohiro Kakizaki, Ryo Fujimoto, Keiichi Okazaki
<i>Crop and Pasture Science</i> 69 ( 1 ) 30 - 39 2018.1
Transposon-mediated mutagenesis and its application to plant breeding
Kanazawa Akira, Fukai Eigo, Miyao Akio, Saze Hidetoshi, Tsukiyama Takuji
Breeding Research 17 ( 2 ) 77 - 87 2015.7
Forward and reverse genetics: The <i>LORE1</i> retrotransposon insertion mutants
Eigo Fukai, Anna Małolepszy, Niels Sandal, Makoto Hayashi, Stig U. Andersen
<i>Compendium of Plant Genomes, The Lotus japonicus Genome</i> 221 - 227 2014.9
Activation of an endogenous retrotransposon associated with epigenetic changes in <i>Lotus japonicus</i>: A tool for functional genomics in legumes Reviewed
Eigo Fukai, Jens Stougaard, Makoto Hayashi
<i>PLANT GENOME</i> 6 ( 3 ) 2013.11
細胞工学別冊 植物細胞工学シリーズ 24 植物のエピジェネティクス 第2章-1 トランスポゾンの制御と進化
深井 英吾, 程 朝陽, 廣近 洋彦
2008.3
ミヤコグサ由来のレトロトランスポゾンＬＯＲＥ１を用いた変異体植物の作製方法
廣近 洋彦, 深井 英吾, 河内 宏, 梅原 洋佐
根こぶ病抵抗性を付与した岩手県ブランドキャベツ品種「CR 春さやか」のスピードブリーディング
2024.7 - 2028.3
System name：オープンイノべーション研究・実用化推進事業（開発研究ステージ 現場課題解決タイプ）
Awarding organization：農業・食品産業技術総合研究機構 生物系特定産業技術研究支援センター
Hybridization-induced epigenetic repression of plant transposable elements
Grant number：23K05163
2023.4 - 2026.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Scientific Research (C)
Awarding organization：Japan Society for the Promotion of Science
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
ブラシカ属作物の春化機構における種内変異，種間変異の遺伝解析とエピゲノム解析
Grant number：21H02163
2021.4 - 2024.3
System name：科学研究費助成事業
Research category：基盤研究(B)
Awarding organization：日本学術振興会
岡崎 桂一, 深井 英吾, 藤本 龍, 板橋 悦子
Grant amount：\17420000 （ Direct Cost: \13400000 、 Indirect Cost：\4020000 ）
A study of mechanisms controlling transposable elements in plant gametophytes
Grant number：19K05967
2019.4 - 2022.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Scientific Research (C)
Awarding organization：Japan Society for the Promotion of Science
Fukai Eigo
Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）
A large proportion of flowering plant genomes is composed of transposable elements (TEs). However, in normally growing plants, their transpositions rarely occur. This is because epigenetic silencing established on TEs is maintained throughout the life cycle of the flowering plant. TEs should be silenced during sexual reproduction processes to prevent their germinal transpositions. On the other hand, it is not well understood how TEs overcome the silencing to be transposed. In this study, we analyzed the silencing mechanisms of TEs using two legume species, Lotus japonicus and soybean.
Study on vernalization mechanism of Brassica crops using newly developed Intromap, a bioinformatic tool to identify alien genomic introgression regions
Grant number：18H02173
2018.4 - 2021.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Scientific Research (B)
Awarding organization：Japan Society for the Promotion of Science
Okazaki Keiichi
Grant amount：\17420000 （ Direct Cost: \13400000 、 Indirect Cost：\4020000 ）
In order to elucidate the vernalization mechanism of the genus Brassica, we did the following studies. We produced C-genome chromosome addition lines using BoFLC2-introgressed B. rapa as a recurrent parent and a cabbage as a c-genome chromosome donor, and characterized those lines with respect to the parental cultivars. In Chinese cabbage, the epigenetic regulation of gene expression at the FLC locus was clarified by RNA-seq, lncRNA analysis, and chromatin immunoprecipitation analysis. In addition, transcriptome analysis was performed on the leaves of the cabbage DH strain with/without low temperature treatment. QTL analysis was performed to determine vernalization characteristics of rapeseed cultivars using F2 populations derived from the crossing between seed vernalization typed rapeseed x green plant vernalization typed rapeseed.
A study of a germline specific plant retrotransposon
Grant number：16K07551
2016.4 - 2019.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Scientific Research (C)
Awarding organization：Japan Society for the Promotion of Science
Fukai Eigo
Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）
Epigenetic regulatory systems preventing the activities of transposable elements (TEs) in flowering plants have been intensively studied. However, it is not well understood how TEs overcome the silencing and transpose. In this study we tried to tackle the question by studying a retrotransposon in the model legume Lotus japonicus called LORE1a, which is active in germline, especially in pollen. The data suggested that LORE1a is active in the postmeiotic pollen grains and that small RNA mediated machinery could be involved in the silencing.
Study on the mechanism of seed- and green plant-vernalization and development of green plant-vernalization type Chinese cabbage
Grant number：15H04433
2015.4 - 2018.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Scientific Research (B)
Awarding organization：Japan Society for the Promotion of Science
Keiichi Okazaki
Grant amount：\16770000 （ Direct Cost: \12900000 、 Indirect Cost：\3870000 ）
We characterized vernalization requirement of 49 strains of oilseed rape, and developed the F2 population of Isuzu-natane (low vernalization requirement) × synthetic B. napus (strong vernalization requirement ). In addition, we developed a line that was introduced the region containing BoFLC 2 region derived from cabbage into the top of chromosome 2 of Chinese cabbage. We showed that the differences of expression level of BrFLC 1/2/3 in cabbage and Chinese cabbage may be related to the diversity of vernalization requirement in cabbage and Chinese cabbage cultivars. The RNA-seq analysis revealed that long non coding RNA was expressed on BoFLC2 locus by low temperature treatment. Our developed Chinese cabbage line showed delay of flowering in the winter cropping pattern, compared with ordinary Chinese cabbage cultivars, indicating that it can be used as a breeding material.
Epigenetic changes in gene expression profiles induced by species crosses.
Grant number：23780011
2011.4 - 2013.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Young Scientists (B)
Awarding organization：Japan Society for the Promotion of Science
FUKAI Eigo
Grant amount：\4030000 （ Direct Cost: \3100000 、 Indirect Cost：\930000 ）
Hybridization between distantly related species has been regarded as a stimulus inducing epigenetic changes in gene expression profiles in the progeny. This study identified gene expression changes in the progeny of crosses between different Lotus species.
内在性レトロトランスポゾンを用いたミヤコグサの遺伝子タギング系の開発
Grant number：06J00900
2006.4 - 2008.3
System name：科学研究費助成事業
Research category：特別研究員奨励費
Awarding organization：日本学術振興会
深井 英吾
Grant amount：\3400000 （ Direct Cost: \3400000 ）
本研究ではミヤコグサ内在のレトロトランスポゾンLORE1,2を用い、ミヤコグサで遺伝子タギング系を確立する事を目的としている。
昨年度に、DNAのシトシンメチル化を制御する遺伝子の発現を低下させたミヤコグサの形質転換体においてLORE1が転移していたことを受け、更なる解析を行った。その結果、組織培養を経て再分化させた植物体の後代の集団において、LORE1の活性化が確率論的に生じていることがが分かった。つまり、LORE1の活性化は組織培養を介して誘導でき、活性化に外来遺伝子の導入は必須ではなく、非形質転換体においてもLORE1の転移を誘導できる事が分かった。
活性化されたLORE1の転移様式を解析した。再分化当代個体のゲノムにおけるLORE1のコピー数増加はこれまでに認められておらず、したがって培養期間中にはLORE1の転移は生じないか、その頻度はきわめて低いと考えられた。一方で、LORE1の新規転移コピーは再分化当代の自殖後代において高頻度に検出された。この事から、LORE1が転移する場は植物体であることが分かった。転移は配偶体的に起きており、この事は、独立の転移挿入数を多く蓄積する必要がある遺伝子タギング系の確立にとって、LORE1が非常に適している事を示す。
以上、人為的なLORE1の誘導に成功した事により、LORE1を利用したミヤコグサの遺伝子タギング系確立が実現可能となった。ミヤコグサ遺伝子タギング系は、植物の新しい分子生物学的研究基盤として、またマメ科植物の農学的研究を加速するツールとして、その利用が期待できる。
アブラナ科自家不和合性制御遺伝子MODの単離と機能解析
Grant number：02J10562
2002.3 - 2004.4
System name：科学研究費助成事業
Research category：特別研究員奨励費
Awarding organization：日本学術振興会
深井 英吾
Grant amount：\2000000 （ Direct Cost: \2000000 ）
本研究では、アブラナ科自家不和合性制御遺伝子MODを単離することを目的としている。今年度は以下の実験を行った。
1.昨年度作成したBrassica rapaの高分子ゲノムDNAライブラリーから、MODと連鎖しているアクアポリン遺伝子MLMを起点に、両方向にゲノムウォーキングを行い、約600kbをカバーするコンティグを得た。
2.得た高分子ゲノムクローンから、MODと連鎖するDNAマーカーを新たに作成した。これらのマーカーを用い、自家不和合性の小国カブS8系統(S8,MOD/MOD)と自家和合性のイエローサルソン(YS,mod/mod)の雑種後代約1500個体について解析を行い、MODとMLMとの相対的な位置関係を明らかにした。この情報に基づき、さらにゲノムウォーキングを行い、新たなマーカーを作成した。最終的にMODの極めて近傍に位置するマーカーを見い出した。
一方、既存のB.rapaのゲノム地図にMODを位置付けた結果、MODの近傍に位置するSSRマーカーを見い出した。このマーカーが座乗する高分子ゲノムクローンを単離し、新たにDNAマーカーを得た。新たに得たマーカーはMODと約2cMの距離に存在した。このマーカーと、これまでのゲノムウォーキングより得ていたマーカーとの間にMODを挟むことができた。
3.昨年度開発した、高分子ゲノムクローン上に存在する遺伝子のcDNA単離法を、MODに最も近傍なDNAマーカーを含む高分子ゲノムクローンに対して行い、柱頭、葯、細葉のそれぞれの組織に由来するcDNAを単離した。これらのうち、MODの発現場所と考えられる柱頭特異的発現を示すものについて、塩基配列を自家不和合性/和合性系統間で比較した。
資源植物生産学概論
フィールド科学インターンシップ
地域交流ｻﾃﾗｲﾄ実習
スタディ・スキルズAIa
植物育種学
スタディ・スキルズAIIa
作物ゲノム学
生物資源科学演習I
科学英語演習
卒業論文II
学科インターンシップ
卒業論文I
生物資源科学演習II
自然科学総論IV
植物生産学演習I
植物エピジェネティクス概論
植物生産学実験実習Ⅳ
植物生産学実験実習Ⅲ
農学入門Ⅱ
スタディ・スキルズA a
生物資源科学演習Ⅰ
農学入門Ⅰ
生物資源科学演習Ⅱ
植物生産実地見学
学科インターンシップ
作物ゲノム学
植物エピジェネティクス概論
農業資源を知る
基礎動植物生産学実験
植物遺伝学
農業生産科学科インターンシップ
基礎生物学
生物資源科学概論
植物分子育種学
生物資源論
植物生産学実験実習Ⅱ
植物生産学実験実習Ⅰ
植物生産学演習Ⅱ
実地見学
植物生産学演習Ⅰ
植物分子遺伝学
