Updated on 2025/05/06

写真a

 
TOMOHIRO UCHIMOTO
 
Organization
Graduate School of Medical and Dental Sciences Specially Appointed Assistant Professor
Title
Specially Appointed Assistant Professor
Profile
(campus)
Specialist of MS-base proteomics of kidney disease and bio-fluid biomarker discovery.
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Degree

  • PhD (Medicine) ( 2005.3   Niigata University )

Research Interests

  • biomarker discovery

  • 応用情報

  • renal diseases

  • 糖尿病性腎症

Research Areas

  • Life Science / Nephrology

Research History

  • Niigata University   Graduate School of Medical and Dental Sciences   Specially Appointed Assistant Professor

    2024.10

  • Niigata University   Graduate School of Medical and Dental Sciences   Specially Appointed Assistant Professor

    2018.4 - 2024.3

  • Niigata University   Institute for Social Innovation and Cooperation   Specially Appointed Assistant Professor

    2016.4 - 2017.3

  • Niigata University   Institute for Research Collaboration and Promotion   Specially Appointed Assistant Professor

    2014.4 - 2016.3

Studying abroad experiences

  • Swiss Federal Institute of Technology in Zurich   Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation

    2012.3 - 2014.3

 

Papers

  • Utilization of the Proteome Data Deposited in SRMAtlas for Validating the Existence of the Human Missing Proteins in GPM

    Amr ELGUOSHY, Yoshitoshi HIRAO, Keiko YAMAMOTO, Bo XU, Naohiko KINOSHITA, Toshiaki MITSUI, Tadashi YAMAMOTO

    Journal of proteome research   2019.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.jproteome.9b00355

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  • Proteome Profiling of Diabetic Mellitus Patient Urine for Discovery of Biomarkers by Comprehensive MS-Based Proteomics Reviewed

    Yoshitoshi Hirao, Suguru Saito, Hidehiko Fujinaka, Shigeru Miyazaki, Bo Xu, Ali F. Quadery, Amr Elguoshy, Keiko Yamamoto, Tadashi Yamamoto

    Proteomes, 6, 9   2018

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  • Proteomic and histopathological characterization of the interface between oral squamous cell carcinoma invasion fronts and non-cancerous epithelia Reviewed

    Tatsuya Abe, Satoshi Maruyama, Manabu Yamazaki, Bo Xu, Hamzah Babkair, Yoshimasa Sumita, Jun Cheng, Tadashi Yamamoto, Takashi Saku

    EXPERIMENTAL AND MOLECULAR PATHOLOGY   102 ( 2 )   327 - 336   2017.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Oral squamous cell carcinomas (SCCs) are frequently associated with pre-invasive lesions including carcinoma in-situ (CIS), and CISs further form lateral interfaces against surrounding normal or dysplastic epithelia (ND). At the interface where keratin (K) 17 positive (+) SCC/CIS cells are in contact with K13 + ND cells, "cell competition" must be evoked between two such different cell types. Thus, the aim of this study was to characterize the histopathology of the SCC/CIS-ND interface and to determine protein profiles around the interface by proteomics. A total of 112 lateral interfaces were collected from 55 CIS and 57 SCC foci, and they were investigated by immunohistochemistry and liquid chromatography-tandem mass spectrometry. The interfaces were morphologically classified into three types: vertical, oblique, and convex. There were several cellular changes characteristic to the interface, including apoptosis and hyaline bodies, which were more emphasized in SCC/CIS sides. The results suggested that ND cells were winners of cell competition against SCC/CIS cells. Then, the interfaces were divided into four vertical segments, and each segment was separately laser-microdissected from tissue sections with immunostaining for K13 or K17; the four segments included SCC/CIS away from(#1) or adjacent to (#2) the interface, and ND adjacent to (#3) or away from (#4) the interface. Proteome analyses revealed approximately 4000 proteins from SCC/CIS sides [#1 and #2] and 2800 proteins from ND sides [#3 and #4]. We quantitatively selected the top 25 proteins including ladinin-1 or interleukin-1 receptor antagonist protein, which were most contrastively increased or decreased in SCC/CIS or ND sides, respectively, and their specific immunohistochemical expression modes were confirmed in tissue sections as well as in cultured SCC cells. These molecules should be involved in the cellular crosstalk toward cell competition at the lateral interface of oral SCC/CIS and would be new candidates for histopathological distinction of oral malignancies. (C) 2017 Elsevier Inc. All rights reserved.

    DOI： 10.1016/j.yexmp.2017.02.018

    Web of Science

    PubMed

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  • Quantitative Urine Proteomics by SWATH-MS Reflects Intricate Metaboric Processing in Cirrhosis Patients Reviewed

    Bo Xu, Yoshitoshi Hirao, Keiko Yamamoto, Ichiei Narita, Tadashi Yamamoto

    American Society of Nephrology, TH-PO1139, P397   2017

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  • Proteomics of glomeruli with IgA nephropathy reveals the concomitant abnormalities of cytoskeleton in the podocytes Reviewed

    Hiroki Yamaguchi, Shin Goto, Yoshitoshi Hirao, Bo Xu, Keiko Yamamoto, Tadashi Yamamoto, Ichiei Narita

    American Society of Nephrology, SA-PO115, P708   2017

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  • Comprehensive data analysis of human ureter proteome Reviewed

    Sameh Magdeldin, Yoshitoshi Hirao, Amr Elguoshy, Bo Xu, Ying Zhang, Hidehiko Fujinaka, Keiko Yamamoto, John R. Yates III, Tadashi Yamamoto

    Data Brief. Mar; 6:853-857   2016

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  • A proteomics glimpse into human ureter proteome Reviewed

    Sameh Magdeldin, Yoshitoshi Hirao, Amr Elguoshy, Bo Xu, Ying Zhang, Hidehiko Fujinaka, Keiko Yamamoto, John R. Yates III, Tadashi Yamamoto

    Proteomics. 16(1), 80-84   2016

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  • Complementary Protein and Peptide OFFGEL Fractionation for High-Throughput Proteomics Analysis Reviewed

    Sameh Magdeldin, Amr Elguoshy, Yutaka Yoshida, Yoshitoshi Hirao, Bo Xu, Ying Zhang, Keiko Yamamoto, Hiroki Takimoto, Hidehiko Fujinaka, Naohiko Kinoshita, Tadashi Yamamoto

    Anal, Chem. 87(16), 8481-8488   2015

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MISC

  • 口腔表在性癌と非癌部粘膜上皮との界面におけるタンパク質動態解析

    阿部 達也, 丸山 智, 山崎 学, 許 波, 隅田 賢正, 程 クン, 山本 格, 朔 敬

    日本病理学会会誌   106 ( 1 )   408 - 408   2017.3

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    Language：Japanese   Publisher：(一社)日本病理学会  

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  • 口腔表在性癌と非癌部粘膜上皮との界面における細胞競合現象の解析

    阿部 達也, 丸山 智, 山崎 学, 許 波, Babkair Hamzah, 隅田 賢正, 程 君, 山本 格, 朔 敬

    日本病理学会会誌   105 ( 1 )   421 - 421   2016.4

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    Language：Japanese   Publisher：(一社)日本病理学会  

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  • 非アルコール性脂肪肝炎におけるDJ-1発現の有用性の検討

    高村 昌昭, 許 波, 山際 訓, 松田 康伸, 野本 実, 若井 俊文, 山本 格, 青柳 豊

    肝臓   55 ( Suppl.2 )   A628 - A628   2014.9

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    Language：Japanese   Publisher：(一社)日本肝臓学会  

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  • 非アルコール性脂肪性肝疾患モデルマウス肝組織のプロテオーム解析による新規バイオマーカーの探索

    高村 昌昭, 許 波, 山際 訓, 松田 康伸, 野本 実, 山本 格, 青柳 豊

    日本臨床分子医学会学術総会プログラム・抄録集   50回   64 - 64   2013.4

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    Language：Japanese   Publisher：日本臨床分子医学会  

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  • 実験的NASHモデルマウス肝組織のプロテオーム解析

    高村 昌昭, 許 波, 嶋田 修一郎, 張 瑩, 吉田 豊, 矢尾板 永信, 山際 訓, 松田 康伸, 野本 実, 山本 格, 青柳 豊

    日本消化器病学会雑誌   109 ( 臨増総会 )   A325 - A325   2012.3

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    Language：Japanese   Publisher：(一財)日本消化器病学会  

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