研究者詳細 - 内許　智博

写真a

ウチモト　トモヒロ

内許　智博

TOMOHIRO UCHIMOTO

所属

医歯学総合研究科特任助教

連絡先

プロフィール
（学内登録）

質量分析機器（MS）ベースの腎臓病プロテオミクス及び生体液バイオマーカー発見における研究者。

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外部リンク

学位

博士（医学）（ 2005年3月新潟大学）

研究キーワード

バイオマーカー
応用情報
腎臓病
糖尿病性腎症

研究分野

ライフサイエンス / 腎臓内科学

経歴

新潟大学医歯学総合研究科特任助教

2024年10月 - 現在
新潟大学医歯学総合研究科特任助教

2018年4月 - 2024年3月
新潟大学地域創生推進機構特任助教

2016年4月 - 2017年3月
新潟大学産学地域連携推進機構特任助教

2014年4月 - 2016年3月

留学歴

スイス連邦工科大学チューリッヒ校頭脳循環を活性化する若手研究者海外派遣プログラム

2012年3月 - 2014年3月

論文

Utilization of the Proteome Data Deposited in SRMAtlas for Validating the Existence of the Human Missing Proteins in GPM

Amr ELGUOSHY, Yoshitoshi HIRAO, Keiko YAMAMOTO, Bo XU, Naohiko KINOSHITA, Toshiaki MITSUI, Tadashi YAMAMOTO

Journal of proteome research 18 ( 12 ) 4197 - 4205 2019年10月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1021/acs.jproteome.9b00355

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The Optimized Workflow for Sample Preparation in LC-MS/MS-Based Urine Proteomics

Suguru Saito, Yoshitoshi Hirao, Ali F. Quadery, Bo Xu, Amr Elguoshy, Hidehiko Fujinaka, Shohei Koma, Keiko Yamamoto, Tadashi Yamamoto

Methods and Protocols 2 ( 2 ) 46 - 46 2019年6月

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掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

The sample condition is an important factor in urine proteomics with stability and accuracy. However, a general protocol of urine protein preparation in mass spectrometry analysis has not yet been established. Here, we proposed a workflow for optimized sample preparation based on methanol/chloroform (M/C) precipitation and in-solution trypsin digestion in LC-MS/MS-based urine proteomics. The urine proteins prepared by M/C precipitation showed around 80% of the protein recovery rate. The samples showed the largest number of identified proteins, which were over 1000 on average compared with other precipitation methods in LC-MS/MS-based urine proteomics. For further improvement of the workflow, the essences were arranged in protein dissolving and trypsin digestion step for the extraction of urine proteins. Addition of Ethylene diamine tetraacetic acid (EDTA) dramatically enhanced the dissolution of protein and promoted the trypsin activity in the digestion step because the treatment increased the number of identified proteins with less missed cleavage sites. Eventually, an optimized workflow was established by a well-organized strategy for daily use in the LC-MS/MS-based urine proteomics. The workflow will be of great help for several aims based on urine proteomics approaches, such as diagnosis and biomarker discovery.

DOI： 10.3390/mps2020046

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Proteomics Analysis of Urine to Examine Physical Effects of Warm Nano Mist Sauna Bathing

Yoshitoshi Hirao, Naohiko Kinoshita, Bo Xu, Suguru Saito, Ali F. Quadery, Amr Elguoshy, Keiko Yamamoto, Tadashi Yamamoto

Healthcare 7 ( 2 ) 71 - 71 2019年5月

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掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Conventional sauna bathing may have some health benefits as it facilitates relaxing, detoxing and promoting metabolism. However, conventional sauna bathing at a high temperature may be harmful for the body by increasing the risk of heart failure. The nano-mist sauna has been developed to provide nano-size water particles at a lower temperature. Hence, nano-mist sauna bathing is expected to be useful for health without the risks that arise at high temperatures. In this study, we performed a comprehensive proteomics analysis of urine samples obtained from healthy volunteers before and after they had taken a sauna bath with nano-mist (n = 10) or with conventional mist (n = 10) daily for two weeks (4 groups). The average numbers of urine proteins identified by liquid chromatography-linked mass chromatography in each group varied from 678 to 753. Interestingly, the protein number was increased after sauna bathing both with nano-mist or with conventional mist. Quantitative analysis indicated that considerable numbers of proteins were obviously up-regulated, with more than two-folds in urine samples after the nano-mist sauna bathing. The KEGG pathway analysis showed significant stimulation of the lysosome pathway (p = 5.89E−6) after the nano-mist bathing, which may indicate the nano-mist sauna bathing promotes metabolism related to the lysosome pathway more efficiently than conventional mist sauna bathing and may provide more health benefits.

DOI： 10.3390/healthcare7020071

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Proteome Profiling of Diabetic Mellitus Patient Urine for Discovery of Biomarkers by Comprehensive MS-Based Proteomics 査読

Yoshitoshi Hirao, Suguru Saito, Hidehiko Fujinaka, Shigeru Miyazaki, Bo Xu, Ali F. Quadery, Amr Elguoshy, Keiko Yamamoto, Tadashi Yamamoto

Proteomes, 6, 9 6 ( 1 ) 9 - 9 2018年

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掲載種別：研究論文（学術雑誌）

DOI： 10.3390/proteomes6010009

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Identification and Validation of Human Missing Proteins and Peptides in Public Proteome Databases: Data Mining Strategy

Amr Elguoshy, Yoshitoshi Hirao, Bo Xu, Suguru Saito, Ali F. Quadery, Keiko Yamamoto, Toshiaki Mitsui, Tadashi Yamamoto

JOURNAL OF PROTEOME RESEARCH 16 ( 12 ) 4403 - 4414 2017年12月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1021/acs.jproteome.7b00423

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Proteomic and histopathological characterization of the interface between oral squamous cell carcinoma invasion fronts and non-cancerous epithelia 査読

Tatsuya Abe, Satoshi Maruyama, Manabu Yamazaki, Bo Xu, Hamzah Babkair, Yoshimasa Sumita, Jun Cheng, Tadashi Yamamoto, Takashi Saku

EXPERIMENTAL AND MOLECULAR PATHOLOGY 102 ( 2 ) 327 - 336 2017年4月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.yexmp.2017.02.018

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PubMed

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Quantitative Urine Proteomics by SWATH-MS Reflects Intricate Metaboric Processing in Cirrhosis Patients 査読

Bo Xu, Yoshitoshi Hirao, Keiko Yamamoto, Ichiei Narita, Tadashi Yamamoto

American Society of Nephrology, TH-PO1139, P397 2017年

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Proteomics of glomeruli with IgA nephropathy reveals the concomitant abnormalities of cytoskeleton in the podocytes 査読

Hiroki Yamaguchi, Shin Goto, Yoshitoshi Hirao, Bo Xu, Keiko Yamamoto, Tadashi Yamamoto, Ichiei Narita

American Society of Nephrology, SA-PO115, P708 2017年

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Why are they missing? : Bioinformatics characterization of missing human proteins

Amr Elguoshy, Sameh Magdeldin, Bo Xu, Yoshitoshi Hirao, Ying Zhang, Naohiko Kinoshita, Yusuke Takisawa, Masaaki Nameta, Keiko Yamamoto, Ali El-Refy, Fawzy El-Fiky, Tadashi Yamamoto

Journal of Proteomics 149 7 - 14 2016年10月

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掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier BV

DOI： 10.1016/j.jprot.2016.08.005

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Avian Podocytes, Which Lack Nephrin, Use Adherens Junction Proteins at Intercellular Junctions. 国際誌

Eishin Yaoita, Hiroko Nishimura, Masaaki Nameta, Yutaka Yoshida, Hiroki Takimoto, Hidehiko Fujinaka, Hiroshi Kawachi, Sameh Magdeldin, Ying Zhang, Bo Xu, Tomizo Oyama, Fujio Nakamura, Tadashi Yamamoto

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 64 ( 1 ) 67 - 76 2016年1月

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記述言語：英語掲載種別：研究論文（学術雑誌）

Nephrin, a major intercellular junction (ICJ) molecule of mammalian podocytes in the renal glomerulus, is absent in the avian genome. We hypothesized that birds use ICJ molecules other than nephrin in their podocytes. Therefore, in the present study, we examined the possible involvement of adherens junction (AJ) proteins in the ICJs of avian podocytes. We found the AJ proteins N-cadherin and α- and β-catenins in podocytes of quail and chickens but not in those of rats, pigs or humans. The AJ proteins were prominent in avian glomerulus-rich fractions in immunoblot analyses, and in immunofluorescence microscopy analyses, they were localized along glomerular capillary walls appearing in at least two staining patterns: weakly diffuse and distinctly granular. Immunoelectron microscopy demonstrated that the significant accumulation of immunogold particles for the AJ proteins were especially evident in avian slit diaphragms and AJs. Furthermore, N-cadherin was found to be expressed in all nephron cells in the early developmental stage but became confined to podocytes during maturation. These results indicate that avian slit diaphragms clearly express AJ proteins as compared with that in the mammal-where AJ proteins are suppressed to an extremely low level-and that avian podocytes are interconnected by AJs per se in addition to slit diaphragms.

DOI： 10.1369/0022155415611708

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Comprehensive data analysis of human ureter proteome 査読

Sameh Magdeldin, Yoshitoshi Hirao, Amr Elguoshy, Bo Xu, Ying Zhang, Hidehiko Fujinaka, Keiko Yamamoto, John R. Yates III, Tadashi Yamamoto

Data Brief. Mar; 6:853-857 6 853 - 857 2016年

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掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.dib.2016.01.050

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A proteomics glimpse into human ureter proteome 査読

Sameh Magdeldin, Yoshitoshi Hirao, Amr Elguoshy, Bo Xu, Ying Zhang, Hidehiko Fujinaka, Keiko Yamamoto, John R. Yates III, Tadashi Yamamoto

Proteomics. 16(1), 80-84 16 ( 1 ) 80 - 84 2016年

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掲載種別：研究論文（学術雑誌）

DOI： 10.1002/pmic.201500214

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その他リンク： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/pmic.201500214
Datasets from label-free quantitative proteomic analysis of human glomeruli with sclerotic lesions. 国際誌

Ying Zhang, Bo Xu, Naohiko Kinoshita, Yutaka Yoshida, Masayuki Tasaki, Hidehiko Fujinaka, Sameh Magdeldin, Eishin Yaoita, Tadashi Yamamoto

Data in brief 4 180 - 5 2015年9月

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記述言語：英語掲載種別：研究論文（学術雑誌）

Human glomeruli with intermediate (i-GS) and advanced (GS) sclerotic lesions as well as the normal control (Nor) were captured from laser microdissection, digested by trypsin and subjected to shotgun LC-MS/MS analysis (LTQ-Orbitrap XL). The label-free quantification was performed using the Normalized Spectral Index (SI N ) to assess the relative molar concentration of each protein identified in a sample. All the experimental data are shown in this article. The data is associated to the research article submitted to Journal of Proteomics [1].

DOI： 10.1016/j.dib.2015.05.013

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Unrestricted modification search reveals lysine methylation as major modification induced by tissue formalin fixation and paraffin embedding

Ying Zhang, Markus Muller, Bo Xu, Yutaka Yoshida, Oliver Horlacher, Frederic Nikitin, Samuel Garessus, Sameh Magdeldin, Naohiko Kinoshita, Hidehiko Fujinaka, Eishin Yaoita, Miki Hasegawa, Frederique Lisacek, Tadashi Yamamoto

Proteomics 15 ( 15 ) 2568 - 2579 2015年8月

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掲載種別：研究論文（学術雑誌）

DOI： 10.1002/pmic.201400454

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Label-free quantitative proteomic analysis reveals strong involvement of complement alternative and terminal pathways in human glomerular sclerotic lesions. 国際誌

Ying Zhang, Bo Xu, Naohiko Kinoshita, Yutaka Yoshida, Masayuki Tasaki, Hidehiko Fujinaka, Sameh Magdeldin, Eishin Yaoita, Tadashi Yamamoto

Journal of proteomics 123 89 - 100 2015年6月

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記述言語：英語掲載種別：研究論文（学術雑誌）

UNLABELLED: Since glomerular sclerosis frequently accompanies various glomerular diseases at the end stages, it is challenging to differentiate ubiquitous biological processes underlying this pathology from those critically involved in specific diseases. Furthermore, in-depth proteomic profile of human glomerular sclerosis remains limited. In this study, human glomeruli with intermediate (i-GS) and advanced (GS) sclerotic lesions, which were excluded from specific renal diseases and assumed to be aging-related, were laser captured from macroscopically normal cortex distant from urological carcinoma, and subjected to label-free quantitative proteomic analysis. We explicate an evident increase of membrane attack complex in i-GS and GS with an up-going tendency, which is accompanied by increasing of inhibitory regulators of alternative and terminal pathways. GO annotation and IPA pathway analysis agree to these results. Proteomic findings are validated by immunohistochemical studies which indicate that alternative and terminal pathways are positively involved in the glomerular sclerosis seen in distinct renal diseases. Furthermore, proteomic analysis also demonstrates remarkable increases of complement factor B in GS and TGF-ß1 in both GS and i-GS. Identification of complement factor B implicates that on-site activation of alternative pathway may occur in injured glomeruli and stepwise increase of TGF-ß1 suggests its contribution to the progression of glomerulosclerosis. BIOLOGICAL SIGNIFICANCE: This study provides in-depth quantitative proteomic profiles of human glomeruli with intermediate and advanced sclerotic lesions. It reveals that the over-expression of alternative and terminal pathway components is significantly involved in human glomerulosclerosis seen in distinct renal diseases. Proteomic identification of the increased TGF-ß1 provides supporting evidence for the role of podocyte apoptosis leading to human glomerulosclerosis.

DOI： 10.1016/j.jprot.2015.03.024

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Complementary Protein and Peptide OFFGEL Fractionation for High-Throughput Proteomics Analysis 査読

Sameh Magdeldin, Amr Elguoshy, Yutaka Yoshida, Yoshitoshi Hirao, Bo Xu, Ying Zhang, Keiko Yamamoto, Hiroki Takimoto, Hidehiko Fujinaka, Naohiko Kinoshita, Tadashi Yamamoto

Anal, Chem. 87(16), 8481-8488 87 ( 16 ) 8481 - 8488 2015年

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掲載種別：研究論文（学術雑誌）

DOI： 10.1021/acs.analchem.5b01911

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Decreased urinary calbindin 1 levels in proteinuric rats and humans with distal nephron segment injuries 査読

Tomoko Iida, Hidehiko Fujinaka, Bo Xu, Ying Zhang, Sameh Magdeldin, Masaaki Nameta, Zan Liu, Yutaka Yoshida, Eishin Yaoita, Shuichi Tomizawa, Akihiko Saito, Tadashi Yamamoto

CLINICAL AND EXPERIMENTAL NEPHROLOGY 18 ( 3 ) 432 - 443 2014年6月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1007/s10157-013-0835-3

Web of Science

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Heparin increasing podocyte-specific gene expressions 査読

Eishin Yaoita, Yutaka Yoshida, Masaaki Nameta, Ying Zhang, Hidehiko Fujinaka, Sameh Magdeldin, Bo Xu, Tadashi Yamamoto

NEPHROLOGY 19 ( 4 ) 195 - 201 2014年4月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1111/nep.12207

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Deep Proteome Mapping of Mouse Kidney Based on OFFGel Prefractionation Reveals Remarkable Protein Post- Translational Modifications

Sameh Magdeldin, Keiko Yamamoto, Yutaka Yoshida, Bo Xu, Ying Zhang, Hidehiko Fujinaka, Eishin Yaoita, John R. Yates, Tadashi Yamamoto

Journal of Proteome Research 13 ( 3 ) 1636 - 1646 2014年3月

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掲載種別：研究論文（学術雑誌）出版者・発行元：American Chemical Society (ACS)

DOI： 10.1021/pr401122m

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Erratum to: Decreased urinary calbindin 1 levels in proteinuric rats and humans with distal nephron segment injuries (Clinical and Experimental Nephrology DOI: 10.1007/s10157-013-0835-3) 査読

Tomoko Iida, Hidehiko Fujinaka, Bo Xu, Ying Zhang, Sameh Magdeldin, Masaaki Nameta, Zan Liu, Yutaka Yoshida, Eishin Yaoita, Shuichi Tomizawa, Akihiko Saito, Tadashi Yamamoto

Clinical and Experimental Nephrology 18 ( 3 ) 444 2014年

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記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Springer-Verlag Tokyo

DOI： 10.1007/s10157-013-0846-0

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Profiling of kidney vascular endothelial cell plasma membrane proteins by liquid chromatography-tandem mass spectrometry.

Zan Liu, Bo Xu, Masaaki Nameta, Ying Zhang, Sameh Magdeldin, Yutaka Yoshida, Keiko Yamamoto, Hidehiko Fujinaka, Eishin Yaoita, Masayuki Tasaki, Yuki Nakagawa, Kazuhide Saito, Kota Takahashi, Tadashi Yamamoto

Clinical and experimental nephrology 17 ( 3 ) 327 - 37 2013年6月

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記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: Vascular endothelial cells (VECs) play crucial roles in physiological and pathologic conditions in tissues and organs. Most of these roles are related to VEC plasma membrane proteins. In the kidney, VECs are closely associated with structures and functions; however, plasma membrane proteins in kidney VECs remain to be fully elucidated. METHODS: Rat kidneys were perfused with cationic colloidal silica nanoparticles (CCSN) to label the VEC plasma membrane. The CCSN-labeled plasma membrane fraction was collected by gradient ultracentrifugation. The VEC plasma membrane or whole-kidney lysate proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and digested with trypsin in gels for liquid chromatography-tandem mass spectrometry. Enrichment analysis was then performed. RESULTS: The VEC plasma membrane proteins were purified by the CCSN method with high yield (approximately 20 μg from 1 g of rat kidney). By Mascot search, 582 proteins were identified in the VEC plasma membrane fraction, and 1,205 proteins were identified in the kidney lysate. In addition to 16 VEC marker proteins such as integrin beta-1 and intercellular adhesion molecule-2 (ICAM-2), 8 novel proteins such as Deltex 3-like protein and phosphatidylinositol binding clathrin assembly protein (PICALM) were identified. As expected, many key functions of plasma membranes in general and of endothelial cells in particular (i.e., leukocyte adhesion) were significantly overrepresented in the proteome of CCSN-labeled kidney VEC fraction. CONCLUSIONS: The CCSN method is a reliable technique for isolation of VEC plasma membrane from the kidney, and proteomic analysis followed by bioinformatics revealed the characteristics of in vivo VECs in the kidney.

DOI： 10.1007/s10157-012-0708-1

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Extracellular heat shock protein A9 is a novel interaction partner of podoplanin in oral squamous cell carcinoma cells 査読

Masayuki Tsuneki, Satoshi Maruyama, Manabu Yamazaki, Bo Xu, Ahmed Essa, Tatsuya Abé, Hamzah Babkair, Jun Cheng, Tadashi Yamamoto, Takashi Saku

Biochemical and Biophysical Research Communications 434 ( 1 ) 124 - 130 2013年4月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.bbrc.2013.03.057

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Murine colon proteome and characterization of the protein pathways 査読

Sameh Magdeldin, Yutaka Yoshida, Huiping Li, Yoshitaka Maeda, Munesuke Yokoyama, Shymaa Enany, Ying Zhang, Bo Xu, Hidehiko Fujinaka, Eishin Yaoita, Sei Sasaki, Tadashi Yamamoto

BioData Mining 5 ( 1 ) 2012年

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1186/1756-0381-5-11

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Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehensive database

Ying Zhang, Yutaka Yoshida, Bo Xu, Sameh Magdeldin, Hidehiko Fujinaka, Zan Liu, Masahito Miyamoto, Eishin Yaoita, Tadashi Yamamoto

Proteome Science 9 2011年8月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1186/1477-5956-9-47

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Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis 査読

Bo Xu, Ying Zhang, Zongjiang Zhao, Yutaka Yoshida, Sameh Magdeldin, Hidehiko Fujinaka, Tamer Ahmed Ismail, Eishin Yaoita, Tadashi Yamamoto

JOURNAL OF PROTEOMICS 74 ( 7 ) 1022 - 1029 2011年6月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.jprot.2011.03.001

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Novel expression of claudin-5 in glomerular podocytes

Ryo Koda, Linning Zhao, Eishin Yaoita, Yutaka Yoshida, Sachiko Tsukita, Atsushi Tamura, Masaaki Nameta, Ying Zhang, Hidehiko Fujinaka, Sameh Magdeldin, Bo Xu, Ichiei Narita, Tadashi Yamamoto

Cell and Tissue Research 343 ( 3 ) 637 - 648 2011年3月

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掲載種別：研究論文（学術雑誌）出版者・発行元：Springer Science and Business Media LLC

DOI： 10.1007/s00441-010-1117-y

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その他リンク： http://link.springer.com/article/10.1007/s00441-010-1117-y/fulltext.html
"All and None" refining strategy; Fishing your correct protein from proteomics ocean 査読

Sameh Magdeldin, Yutaka Yoshida, Ying Zhang, Bo Xu, Eishin Yaoita, Tadashi Yamamoto

Journal of Proteomics and Bioinformatics 4 ( 6 ) 123 - 124 2011年

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.4172/jpb.1000178

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Differential Proteomic Shotgun Analysis Elucidates Involvement of Water Channel Aquaporin 8 in Presence of alpha-Amylase in the Colon 査読

Sameh Magdeldin, Huiping Li, Yutaka Yoshida, Ichiro Satokata, Yoshitaka Maeda, Munesuke Yokoyama, Shymaa Enany, Ying Zhang, Bo Xu, Hidehiko Fujinaka, Eishin Yaoita, Tadashi Yamamoto

JOURNAL OF PROTEOME RESEARCH 9 ( 12 ) 6635 - 6646 2010年12月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1021/pr100789v

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Comparison of two dimensional electrophoresis mouse colon proteomes before and after knocking out Aquaporin 8 査読

Sameh Magdeldin, Huiping Li, Yutaka Yoshida, Shymaa Enany, Ying Zhang, Bo Xu, Hidehiko Fujinaka, Eishin Yaoita, Tadashi Yamamoto

Journal of Proteomics 73 ( 10 ) 2031 - 2040 2010年9月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.jprot.2010.06.010

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Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney 査読

Ying Zhang, Yutaka Yoshida, Masaaki Nameta, Bo Xu, Izumi Taguchi, Takako Ikeda, Hidehiko Fujinaka, Sameh Magdeldin Mohamed, Hiroyasu Tsukaguchi, Yutaka Harita, Eishin Yaoita, Tadashi Yamamoto

NEPHROLOGY DIALYSIS TRANSPLANTATION 25 ( 6 ) 1785 - 1795 2010年6月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1093/ndt/gfp697

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Dietary supplementation with arachidonic acid but not eicosapentaenoic or docosahexaenoic acids alter lipids metabolism in C57BL/6J mice

Sameh Magdeldin, Yaser Elewa, Takako Ikeda, Junko Ikei, Ying Zhang, Bo Xu, Masaaki Nameta, Hidehiko Fujinaka, Yutaka Yoshida, Eishin Yaoita, Tadashi Yamamoto

GENERAL PHYSIOLOGY AND BIOPHYSICS 28 ( 3 ) 266 - 275 2009年9月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.4149/gpb_2009_03_266

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Identification and characterization of major proteins carrying ABO blood group antigens in the human kidney. 国際誌

Masayuki Tasaki, Yutaka Yoshida, Masahito Miyamoto, Masaaki Nameta, Lino M Cuellar, Bo Xu, Ying Zhang, Eishin Yaoita, Yuki Nakagawa, Kazuhide Saito, Tadashi Yamamoto, Kota Takahashi

Transplantation 87 ( 8 ) 1125 - 33 2009年4月

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記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: It is generally admitted that ABO(H) blood group antigens are linked to lipids and proteins. Although glycolipids carrying ABO antigens have been well characterized in human kidneys, glycoproteins carrying ABO antigens are largely unknown, and their molecular properties remain to be elucidated. METHODS: All the blood group A antigen-linked proteins in human kidney could be solubilized and captured on immobilized Helix pomatia lectin that recognizes A antigens. These proteins were separated on SDS-PAGE gels. The gel pieces containing protein bands immunoreactive with anti-A antibody were excised, in-gel digested with trypsin, and analyzed by nanoLC tandem mass spectrometer. Protein candidates that carry ABO antigens were confirmed by immunoprecipitation and double-labeled immunofluorescense microscopy. RESULTS: All the glycoproteins carrying ABO antigens were found to be Asn-linked glycoproteins, and presented as multiple bands on SDS-PAGE with molecular masses ranging from 60 to 270 kDa. The protein bands were subjected for mass spectrometric analysis, which identified 121 distinct proteins with high confidence. Of the identified proteins, 55 N-glycosylated, membrane proteins were selected as glycoprotein candidates that carry ABO antigens. Among them, most abundantly expressed proteins as estimated by the number of peptide matches in the MS spectrometric analysis, such as platelet endothelial cell adhesion molecule 1, plasmalemmal vesicle-associated protein, and von Willebrand factor, were further characterized. CONCLUSIONS: Several glycoproteins were identified that represented major glycoproteins carrying ABO antigens in the human kidney, which exhibited distinct features in localization to most of vascular endothelial cells.

DOI： 10.1097/TP.0b013e31819e0054

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Mesangial Cells Connected by the N-Cadherin-Catenin System in the Rat Kidney 査読

Masaaki Nameta, Eishin Yaoita, Nobutaka Kato, Linning Zhao, Ying Zhang, Hidehiko Fujinaka, Bo Xu, Yutaka Yoshida, Tadashi Yamamoto

NEPHRON EXPERIMENTAL NEPHROLOGY 112 ( 4 ) E92 - E98 2009年

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1159/000224797

Web of Science

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Claudin-6 localized in tight junctions of rat podocytes 査読

Linning Zhao, Eishin Yaoita, Masaaki Nameta, Ying Zhang, Lino Munoz Cuellar, Hidehiko Fujinaka, Bo Xu, Yutaka Yoshida, Katsuyoshi Hatakeyama, Tadashi Yamamoto

AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY 294 ( 6 ) R1856 - R1862 2008年6月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1152/ajpregu.00862.2007

Web of Science

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In-depth proteomic profiling of the normal human kidney glomerulus using two-dimensional protein prefractionation in combination with liquid chromatography-tandem mass spectrometry. 国際誌

Masahito Miyamoto, Yutaka Yoshida, Izumi Taguchi, Yoshimi Nagasaka, Masayuki Tasaki, Ying Zhang, Bo Xu, Masaaki Nameta, Hiroshi Sezaki, Lino M Cuellar, Tetsuo Osawa, Hideo Morishita, Shigeki Sekiyama, Eishin Yaoita, Kenjiro Kimura, Tadashi Yamamoto

Journal of proteome research 6 ( 9 ) 3680 - 90 2007年9月

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記述言語：英語掲載種別：研究論文（学術雑誌）

The kidney glomerulus plays a pivotal role in ultrafiltration of plasma into urine and also is the locus of kidney disease progressing to chronic renal failure. We have focused proteomic analysis on the glomerulus that is most proximal to the disease locus. In the present study, we aimed to provide a confident, in-depth profiling of the glomerulus proteome. The glomeruli were highly purified from the kidney cortex from a male, 68-year-old patient who underwent nephroureterectomy due to ureter carcinoma. The patient was normal in clinical examinations including serum creatinine and urea levels and liver function, and did not receive any chemotherapy and radiotherapy. The cortical tissue was histologically normal, and no significant deposition of immunoglobulins and complement C3 was observed. We employed a novel strategy of protein separation using 1D (SDS-PAGE) and 2D (solution-phase IEF in combination with SDS-PAGE) prefractionation prior to the shotgun analysis with LC-MS/MS. The protein prefractionation produced 90 fractions, and eventually provided a confident set of identified proteins consisting of 6686 unique proteins (3679 proteins with two or more peptide matches and 3007 proteins with one peptide match), representing 2966 distinct genes. All the identified proteins were annotated and classified in terms of molecular function and biological process, compiled into 1D and 2D protein arrays, consisting of 15 and 75 sections, corresponding to the protein fractions which were defined by MW and pI range, and deposited on a Web-based database (http://www.hkupp.org). The most remarkable feature of the glomerulus proteome was a high incidence of identification of cytoskeleton-related proteins, presumably reflecting the well-developed, cytoskeletal organization of glomerular cells related to their physiological functions.

PubMed

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Localization of tyrosine-phosphorylated proteins in the normal rat kidney

Ying Zhang, Yutaka Yoshida, Bo Xu, Masaaki Nameta, Masahito Miyamoto, Eishin Yaoita, Tadashi Yamamoto

Acta Medica et Biologica 55 ( 1 ) 1 - 7 2007年3月

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記述言語：英語掲載種別：研究論文（学術雑誌）

Scopus

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Two-dimensional electrophoretic profiling of normal human kidney glomerulus proteome and construction of an extensible markup language (XML)-based database

Yutaka Yoshida, Kenji Miyazaki, Junichi Kamiie, Masao Sato, Seiji Okuizumi, Akihisa Kenmochi, Ken'ichi Kamijo, Takuji Nabetani, Akira Tsugita, Bo Xu, Ying Zhang, Eishin Yaoita, Tetsuo Osawa, Tadashi Yamamoto

Proteomics 5 ( 4 ) 1083 - 1096 2005年3月

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記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）

DOI： 10.1002/pmic.200401075

Scopus

PubMed

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MISC

口腔表在性癌と非癌部粘膜上皮との界面におけるタンパク質動態解析

阿部達也, 丸山智, 山崎学, 許波, 隅田賢正, 程クン, 山本格, 朔敬

日本病理学会会誌 106 ( 1 ) 408 - 408 2017年3月

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記述言語：日本語出版者・発行元：(一社)日本病理学会

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口腔表在性癌と非癌部粘膜上皮との界面における細胞競合現象の解析

阿部達也, 丸山智, 山崎学, 許波, Babkair Hamzah, 隅田賢正, 程君, 山本格, 朔敬

日本病理学会会誌 105 ( 1 ) 421 - 421 2016年4月

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記述言語：日本語出版者・発行元：(一社)日本病理学会

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非アルコール性脂肪肝炎におけるDJ-1発現の有用性の検討

高村昌昭, 許波, 山際訓, 松田康伸, 野本実, 若井俊文, 山本格, 青柳豊

肝臓 55 ( Suppl.2 ) A628 - A628 2014年9月

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記述言語：日本語出版者・発行元：(一社)日本肝臓学会

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Basics and recent advances of two dimensional-polyacrylamide gel electrophoresis

Sameh Magdeldin, Shymaa Enany, Yutaka Yoshida, Bo Xu, Ying Zhang, Zam Zureena, Ilambarthi Lokamani, Eishin Yaoita, Tadashi Yamamoto

Clinical Proteomics 11 ( 1 ) 2014年

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記述言語：英語掲載種別：書評論文，書評，文献紹介等出版者・発行元：BioMed Central Ltd.

DOI： 10.1186/1559-0275-11-16

Scopus

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非アルコール性脂肪性肝疾患モデルマウス肝組織のプロテオーム解析による新規バイオマーカーの探索

高村昌昭, 許波, 山際訓, 松田康伸, 野本実, 山本格, 青柳豊

日本臨床分子医学会学術総会プログラム・抄録集 50回 64 - 64 2013年4月

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記述言語：日本語出版者・発行元：日本臨床分子医学会

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Comparative proteomic analysis of the liver in a murine model of non-alcoholic steatohepatitis

Masaaki Takamura, Bo Xu, Satoshi Yamagiwa, Yasunobu Matsuda, Shuichiro Shimada, Ying Zhang, Yutaka Yoshida, Eishin Yaoita, Minoru Nomoto, Tadashi Yamamoto, Yutaka Aoyagi

HEPATOLOGY 56 855A - 855A 2012年10月

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記述言語：英語掲載種別：研究発表ペーパー・要旨（国際会議）

Web of Science

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Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney (vol 25, pg 1785, 2010)

Ying Zhang, Yutaka Yoshida, Masaaki Nameta, Bo Xu, Izumi Taguchi, Takako Ikeda, Hidehiko Fujinaka, Sameh Magdeldin, Hiroyasu Tsukaguchi, Yutaka Harita, Eishin Yaoita, Tadashi Yamamoto

NEPHROLOGY DIALYSIS TRANSPLANTATION 27 ( 6 ) 2606 - 2606 2012年6月

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記述言語：英語

DOI： 10.1093/ndt/gfs155

Web of Science

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実験的NASHモデルマウス肝組織のプロテオーム解析

高村昌昭, 許波, 嶋田修一郎, 張瑩, 吉田豊, 矢尾板永信, 山際訓, 松田康伸, 野本実, 山本格, 青柳豊

日本消化器病学会雑誌 109 ( 臨増総会 ) A325 - A325 2012年3月

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記述言語：日本語出版者・発行元：(一財)日本消化器病学会

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Human kidney glomerulus proteome and biomarker discovery of kidney diseases

Yutaka Yoshida, Masahito Miyamoto, Izumi Taguchi, Bo Xu, Ying Zhang, Eishin Yaoita, Hidehiko Fujinaka, Tadashi Yamamoto

PROTEOMICS CLINICAL APPLICATIONS 2 ( 3 ) 420 - 427 2008年3月

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記述言語：英語掲載種別：書評論文，書評，文献紹介等

DOI： 10.1002/prca.200780016

Web of Science

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